CN104663730A - Microbial herbicide for aquatic plant - Google Patents
Microbial herbicide for aquatic plant Download PDFInfo
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- CN104663730A CN104663730A CN201310639825.3A CN201310639825A CN104663730A CN 104663730 A CN104663730 A CN 104663730A CN 201310639825 A CN201310639825 A CN 201310639825A CN 104663730 A CN104663730 A CN 104663730A
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- bacterial classification
- water plants
- microbial herbicide
- fermentation
- plants microbial
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a microbial herbicide for aquatic plant. A bacterial strain is prepared from diphtheria bacillus, curvularia lunata, streptomyces scabies and lactobacillus plantarum.
Description
Technical field
The present invention relates to a kind of water plants microbial herbicide containing microorganism and preparation method thereof, specifically, relate between different strain and carry out by synergistic function a kind of microorganism organic agricultural chemicals that effective compound produces, belong to agricultural intensive production technology.
Background technology
Sulfonylurea herbicide is the class weed killer herbicide that usage amount is maximum in the world at present, it studies successfully by du pont company in 20 century 70s, and it is grand to develop cornfield herbicidal chlorine sulphur first in nineteen eighty-two, develop again many efficient, wide spectrum, low toxicity, high selectivity sulfonylurea herbicide afterwards.But the most distinct issues of sulfonylurea herbicide are the long residual drug injury problems to succession crop, because the migration leaching of such weed killer herbicide is more weak, when it is residual reach doses after will cause poisoning to succession crop.The field of some Long-Time Service long residual herbicides have also appeared the situation of herbicide residue amount accumulation, has a strong impact on the growth of succession crop, defines the phenomenon in " cancer field ".And there is the agricultural chemicals of Hazardous, also change soil, make alkalization of soils, pollute environment, cause serious life too uneven, cause a lot of small animal and birds extinction, its residual harmful substance also drastically influence the healthy of the mankind and multiplies with the species of the boundless universe.
And biopesticide is a kind of class pesticidal preparations utilizing biological living or its metabolite to prevent and treat pests such as insect, germ, weeds, nematode, muroids, biopesticide comprises four classes such as microbial pesticide, botanical pesticide, animal pesticide and genetically modified organism agricultural chemicals, biopesticide has following advantage compared with chemical pesticide: 1) selectivity is strong, to people and animals and various beneficial organism safer; 2) little to eco-environmental impact.
Summary of the invention
Water plants microbial herbicide that the object of the present invention is to provide a kind of organic composite and preparation method thereof.Microbial herbicide provided by the invention is with the bacterial classification with strong adaptability, good stability, the not easily feature such as aging extracted at occurring in nature, and the synergy between Appropriate application microorganism, by producing the high-quality organic agriculture microbial herbicide that fermentation technique is produced.
For achieving the above object, the present invention by the following technical solutions:
Water plants microbial herbicide, adopts corynebacterium diphtheriae (diphtheria bacillus), new long-radius elbow spore (Curvularia lunata), shot hole streptomycete (Streptomyces scabie) and Lactobacillus plantarum (Lactobacillus plantarum) to prepare bacterial classification.
Water plants microbial herbicide provided by the invention, the mass percent of described bacterial classification is: corynebacterium diphtheriae 10 ~ 25%, new long-radius elbow spore 25 ~ 30%, shot hole streptomycete 15 ~ 40%, Lactobacillus plantarum 5 ~ 15%.
Water plants microbial herbicide provided by the invention, preferably, the mass percent of described bacterial classification is: corynebacterium diphtheriae 20%, new long-radius elbow spore 28%, shot hole streptomycete 38%, Lactobacillus plantarum 14%.
Present invention also offers the preparation method of above-mentioned water plants microbial herbicide, it is characterized in that:
1) inoculate: the solid culture medium of preparation bacterial classification, strictly carry out sterile working after sterilizing, be forwarded to flat board from inclined-plane, cultivate 1 day at temperature 28 DEG C;
2) Spawn incubation: the dull and stereotyped picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of liquid nutrient medium, sterile working, 28 DEG C, 130r/min cultivates 36 ~ 72h;
3) strain fermentation: by cultured strain inoculation in the 500mL conical flask filling liquid nutrient medium, sterile working, 28 DEG C, 130r/min cultivates 36 ~ 72h;
4) mixed culture fermentation: the mass percent of each bacterial classification by described bacterial classification is mixed, being inoculated in by mixed bacterium liquid fills in the fermentation tank of fermentation medium, pH6.0 ~ 8.0, and inoculum concentration is 1 ~ 5%, cultivate 4 ~ 7 days at 28 DEG C, make bacterium number in zymotic fluid reach 10 ~ 15 × 10
8individual/mL, namely obtains described water plants microbial herbicide.
The preparation method of water plants microbial herbicide provided by the invention, preferably, consisting of of described fermentation medium: glucose 5 ~ 8g/L, honey 20 ~ 80g/L, MC peptone 0.5 ~ 3g/L, beef extract 1 ~ 5g/L, KH
2pO
41 ~ 5g/L, urea 1 ~ 5g/L, K
2hPO
45 ~ 20g/L, MgSO
47H
2o5 ~ 20g/L, NaCl5 ~ 20g/L, (NH
4)
2sO
45 ~ 20g/L, all the other are distilled water.
Compared to prior art, organic composite water plants provided by the invention microbial herbicide adopts corynebacterium diphtheriae, new long-radius elbow spore, shot hole streptomycete and Lactobacillus plantarum mixed culture fermentation to be prepared from.Above bacterial classification is respectively through Spawn incubation, and two-stage fermentation, makes bacterium number in zymotic fluid reach 10 ~ 15 × 10
8individual/mL, obtains composite farm microbial herbicide.Product provided by the invention is through verifying on the spot for a long time, and rationally, stable effect, effectively can remove the weeds near water plants to formula, have efficient, stablize, safety, the advantage such as environmental protection.
Embodiment
The following examples can make the present invention of those of ordinary skill in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1
Water plants microbial herbicide, the mass percent of described bacterial classification is:
Embodiment 2
Water plants microbial herbicide, the mass percent of described bacterial classification is:
Embodiment 3
Water plants microbial herbicide, the mass percent of described bacterial classification is:
Embodiment 4
The preparation method of the water plants microbial herbicide that embodiment 1 ~ 3 provides, carries out according to the following steps:
1) inoculate: the solid culture medium of preparation bacterial classification, strictly carry out sterile working after sterilizing, be forwarded to flat board from inclined-plane, cultivate 1 day at temperature 28 DEG C;
2) Spawn incubation: the dull and stereotyped picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of liquid nutrient medium, sterile working, 28 DEG C, 130r/min cultivates 72h;
3) strain fermentation: by cultured strain inoculation in the 500mL conical flask filling liquid nutrient medium, sterile working, 28 DEG C, 130r/min cultivates 72h;
4) mixed culture fermentation: the mass percent of each bacterial classification by described bacterial classification mixed, be inoculated in by mixed bacterium liquid and fill in the fermentation tank of fermentation medium, pH6.0 ~ 8.0, inoculum concentration is cultivate 6 days at 5%, 28 DEG C, makes bacterium number in zymotic fluid reach 10 ~ 15 × 10
8individual/mL, namely obtains described water plants microbial herbicide.
Consisting of of described fermentation medium: glucose 5 ~ 8g/L, honey 20 ~ 80g/L, MC peptone 0.5 ~ 3g/L, beef extract 1 ~ 5g/L, KH
2pO
41 ~ 5g/L, urea 1 ~ 5g/L, K
2hPO
45 ~ 20g/L, MgSO
47H
2o5 ~ 20g/L, NaCl5 ~ 20g/L, (NH
4)
2sO
45 ~ 20g/L, all the other are distilled water.
Claims (5)
1. water plants microbial herbicide, is characterized in that: adopt corynebacterium diphtheriae (diphtheria bacillus), new long-radius elbow spore (Curvularia lunata), shot hole streptomycete (Streptomyces scabie) and Lactobacillus plantarum (Lactobacillus plantarum) to prepare bacterial classification.
2. water plants microbial herbicide according to claim 1, is characterized in that the mass percent of described bacterial classification is:
3. water plants microbial herbicide according to claim 2, is characterized in that: the mass percent of described bacterial classification is: corynebacterium diphtheriae 20%, new long-radius elbow spore 28%, shot hole streptomycete 38%, Lactobacillus plantarum 14%.
4. the preparation method of water plants microbial herbicide according to claim 1, is characterized in that:
1) inoculate: the solid culture medium of preparation bacterial classification, strictly carry out sterile working after sterilizing, be forwarded to flat board from inclined-plane, cultivate 1 day at temperature 28 DEG C;
2) Spawn incubation: the dull and stereotyped picking list bacterium colony grown is forwarded to and is equipped with in the 50mL conical flask of liquid nutrient medium, sterile working, 28 DEG C, 130r/min cultivates 36 ~ 72h;
3) strain fermentation: by cultured strain inoculation in the 500mL conical flask filling liquid nutrient medium, sterile working, 28 DEG C, 130r/min cultivates 36 ~ 72h;
4) mixed culture fermentation: the mass percent of each bacterial classification by described bacterial classification is mixed, being inoculated in by mixed bacterium liquid fills in the fermentation tank of fermentation medium, pH6.0 ~ 8.0, and inoculum concentration is 1 ~ 5%, cultivate 4 ~ 7 days at 28 DEG C, make bacterium number in zymotic fluid reach 10 ~ 15 × 10
8individual/mL, namely obtains described water plants microbial herbicide.
5. the preparation method of water plants microbial herbicide according to claim 4, is characterized in that: consisting of of described fermentation medium: glucose 5 ~ 8g/L, honey 20 ~ 80g/L, MC peptone 0.5 ~ 3g/L, beef extract 1 ~ 5g/L, KH
2pO
41 ~ 5g/L, urea 1 ~ 5g/L, K
2hPO
45 ~ 20g/L, MgSO
47H
2o5 ~ 20g/L, NaCl5 ~ 20g/L, (NH
4)
2sO
45 ~ 20g/L, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310639825.3A CN104663730A (en) | 2013-11-29 | 2013-11-29 | Microbial herbicide for aquatic plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310639825.3A CN104663730A (en) | 2013-11-29 | 2013-11-29 | Microbial herbicide for aquatic plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104663730A true CN104663730A (en) | 2015-06-03 |
Family
ID=53299736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310639825.3A Pending CN104663730A (en) | 2013-11-29 | 2013-11-29 | Microbial herbicide for aquatic plant |
Country Status (1)
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| CN (1) | CN104663730A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105594767A (en) * | 2016-03-07 | 2016-05-25 | 鞍山禾瑞生物科技有限公司 | Liquid state composite biological multivariable control paddy herbicide and preparation method thereof |
| CN106396853A (en) * | 2016-08-26 | 2017-02-15 | 山东胜伟园林科技有限公司 | Modifying fertilizer suitable for saline and alkaline land, and production method thereof |
-
2013
- 2013-11-29 CN CN201310639825.3A patent/CN104663730A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105594767A (en) * | 2016-03-07 | 2016-05-25 | 鞍山禾瑞生物科技有限公司 | Liquid state composite biological multivariable control paddy herbicide and preparation method thereof |
| CN106396853A (en) * | 2016-08-26 | 2017-02-15 | 山东胜伟园林科技有限公司 | Modifying fertilizer suitable for saline and alkaline land, and production method thereof |
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Application publication date: 20150603 |