CN104651040A - Method for degumming fragrant rap oil through enzymic process - Google Patents

Method for degumming fragrant rap oil through enzymic process Download PDF

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Publication number
CN104651040A
CN104651040A CN201510049752.1A CN201510049752A CN104651040A CN 104651040 A CN104651040 A CN 104651040A CN 201510049752 A CN201510049752 A CN 201510049752A CN 104651040 A CN104651040 A CN 104651040A
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China
Prior art keywords
oil
phospholipase
giving
fragrant
strong fragrance
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510049752.1A
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Chinese (zh)
Inventor
龚任
周俊梅
李维琳
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State's Grain Wuhan Co Ltd Of Scientific Research Designing Institute
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State's Grain Wuhan Co Ltd Of Scientific Research Designing Institute
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Priority to CN201510049752.1A priority Critical patent/CN104651040A/en
Publication of CN104651040A publication Critical patent/CN104651040A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C11B3/04—Refining fats or fatty oils by chemical reaction with acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Fats And Perfumes (AREA)
  • Edible Oils And Fats (AREA)

Abstract

The invention discloses a method for degumming fragrant rap oil through an enzymic process. The method is characterized by comprising the following steps: heating the squeezed fragrant crude rap oil without impurities to about 40 DEG C, adding a 50% citric acid aqueous solution according to 0.1% by weight of the oil, mixing the 50% citric acid aqueous solution with the fragrant crude rap oil, mixing and stirring at a rate of 50-60r/min, and stirring for one hour; adding soft water according to 1.8% by weight of the oil, adding a phospholipase A, phospholipase B or phospholipase C solution diluted to 100 times according to 0.13% of the total mass of oil and water, simultaneously stirring and reacting for 60 minutes at a rate of 40-50r/min under the temperature of 30-50 DEG C, filtering or centrifugally separating, and drying to obtain the finished product of the fragrant rap oil.

Description

A kind of method of giving off a strong fragrance rapeseed oil enzymatic degumming
Technical field
The present invention relates to the method for giving off a strong fragrance degumming of rape oil, specifically one phospholipase A 1or phospholipase A 2remove the method for phospholipid colloid in giving off a strong fragrance rapeseed oil.
Background technology
The existing method preparing giving off a strong fragrance rapeseed oil for raw material comes unstuck with giving off a strong fragrance crude rapeseed oil adopts hydration degum and acid system to come unstuck.It is the wetting ability utilizing the colloids such as phosphatide that water law is come unstuck, and under agitation adds in oil by hot water, and phosphatide water-swelling is condensed, other colloids in the grease energy absorb oil after expansion, and the solubleness of colloid in oil is reduced, through natural subsidence and separating of oil.It is first heated by crude oil that acid system comes unstuck, and then fully mixes rear reaction with phosphoric acid or citric acid solution, then adds water and be then separated.
Hydration degum can only remove hydratability phosphatide, and non-aqueous phosphatide is difficult to remove, and the grease after hydration degum is generally still containing a certain amount of phosphorus.No matter be that hydration degum or acid system come unstuck, all well can not remove non-hydratable phospholipid, affect the effect of coming unstuck.If degumming effect is bad, the burden of follow-up decolouring, depickling, deodorization will be increased the weight of, increase the consumption of oil and fat refining loss and subsidiary material.Cause filtration difficulty, equipment coking, Clay consumption amount increases, refining yield is low, and phosphorus content is high often along with more metal ion, causes oil easily to become sour and color reversion phenomenon, affects oil quality and economic benefit., easily have a negative impact to equipment, as equipment coking affects heat-transfer effect, filter screen easily blocks and affects filtration velocity and cause decolouring difficulty, the increase of carclazyte consumption etc. meanwhile.
Summary of the invention
The object of this invention is to provide a kind of technological process short, the method for giving off a strong fragrance vegetable seed degummed oil is prepared in low the coming unstuck with Phospholipid hydrolase of production cost.
The present invention is realized by some technical measures:
1, a method for giving off a strong fragrance rapeseed oil enzymatic degumming, is characterized in that, comprises the following steps:
(1) by through squeezing, filter, giving off a strong fragrance crude rapeseed oil that removal of impurities is obtained at 40 DEG C time, the concentration adding weight of oil 0.4% is the aqueous citric acid solution of 50%, with 50 ~ 60r/min speed mix and blend 60min, obtains the crude oil being mixed with acid solution;
(2) crude oil being mixed with acid solution is added soft water mixing in the ratio of weight of oil 1.8%, add the phospholipase A of dilution 100 times or phospholipase B or Phospholipase C in oil and the ratio of water total mass 0.13% simultaneously, and with 40 ~ 50r/min speed stirring reaction 60mi minute n at 30 DEG C ~ 50 DEG C temperature, after filtration or centrifugation remove colloid, then drying obtains giving off a strong fragrance rapeseed oil finished product.
Below describe the present invention.
A method for giving off a strong fragrance rapeseed oil enzymatic degumming, comprises the following steps:
(1) by through frying seed---the giving off a strong fragrance crude rapeseed oil that obtains of squeezing oil-producing technique carries out pre-treatment removal of impurities;
(2) with acidic solution, the rapeseed oil after removal of impurities is uniformly mixed, is fully mixed with the crude oil of acid solution;
(3) to add water and enzyme comes unstuck to crude rapeseed oil, stir mixed after carry out standing separation or centrifugation, the fluid removed after colloid is degummed oil product.
Above-mentioned crude rapeseed oil leaves standstill and filters out wherein after impurities, be warming up to 40 DEG C about-45 DEG C in weight of oil 0.4% ratio to add concentration be that 50% aqueous citric acid solution mixes with giving off a strong fragrance crude rapeseed oil, mix and blend speed is 50-60r/min, and churning time is 1 hour.After adding soft water mixing in the ratio of weight of oil 1.8% again, add the phospholipase A of dilution 100 times or phospholipase B or Phospholipase C in oil and the ratio of water total mass 0.013% simultaneously, and with 40-50r/min speed stirring reaction 60 minutes at 30 DEG C of-50 DEG C of temperature.
Vegetable seed giving off a strong fragrance oil production obtained above after filtration or centrifugation, obtain giving off a strong fragrance rapeseed oil products after drying.
The beneficial effect that the present invention has is:
The present invention compared with prior art, effect more better than traditional Degumming method technique can be obtained: adopt enzyme method technique to come unstuck aborning, oil sample is through thermal test, oil colours obviously shoals, without precipitate, phosphatide (hydrated phospholipid and non-hydratable phospholipid) residual minim is described, is enough to the condition meeting physical refining process requirement; Enzymatic degumming eliminates washing step, thus avoids because washing the oily loss caused; After adopting enzymatic degumming, reduce the loss of alkali refining; Adopt enzymatic degumming technique, the oil foot amount after centrifugation reduces greatly, and the butt oleaginousness of oil foot is very low; Adopt enzymatic degumming technique, the cost of raw materials is lower than chemical degumming technique.
The present invention not only has above-mentioned advantage, and when adopting Luzhou-flavor crude rapeseed oil as raw material, can prepare strong fragrance and meeting the giving off a strong fragrance rapeseed oil products of people to local flavor demand.
Accompanying drawing explanation
Accompanying drawing is present invention process schematic flow sheet.
Embodiment
The present invention is further illustrated below in conjunction with drawings and Examples:
Embodiment 1:
1. will fry seed through cleaning, squeezing oil-producing technique obtains giving off a strong fragrance crude rapeseed oil and leave standstill centrifugation or mechanical filter after 24 hours and go out wherein contained most of impurity, impurity elimination crude oil is heated to 40 DEG C-45 DEG C through interchanger in enzymatic vessel.
2. citric acid solid is dissolved in soft water be made into mass concentration be 50% citric acid solution for subsequent use in sour tank.
3. add the citric acid solution of 50% in the ratio of weight of oil 0.4%, carry out stirring and mix, stirring mixed speed is 50r/min, and the time is 60 minutes.
4. the oil in acid-respons tank and sour mixed solution are proceeded in enzyme reaction tank, add soft water, soft water water temperature is higher than oil temperature 5 DEG C, and soft water add-on is about 1.8% of oil mass, stir and be fully mixed evenly, then add the phospholipase A solution of dilution 100 times in the ratio of 0.13 % of total mass.Enzyme-added liquid limit, limit is stirred, and stirring velocity is 40r/min, and after 60 minutes stirring reaction time, standing sedimentation isolates colloid, obtains the vegetable seed giving off a strong fragrance oil that comes unstuck after drying.
The product index of the giving off a strong fragrance vegetable seed degummed oil obtained is:
Moisture≤0.2%
Acid value≤2mg/g
Phosphorus content≤20ppm.

Claims (1)

1. a method for giving off a strong fragrance rapeseed oil enzymatic degumming, is characterized in that, comprises the following steps:
(1) by through squeezing, filter, giving off a strong fragrance crude rapeseed oil that removal of impurities is obtained at 40 DEG C time, the concentration adding weight of oil 0.4% is the aqueous citric acid solution of 50%, with 50 ~ 60r/min speed mix and blend 60min, obtains the crude oil being mixed with acid solution;
(2) crude oil being mixed with acid solution is added soft water mixing in the ratio of weight of oil 1.8%, add the phospholipase A of dilution 100 times or phospholipase B or Phospholipase C in oil and the ratio of water total mass 0.13% simultaneously, and with 40 ~ 50r/min speed stirring reaction 60mi minute at 30 DEG C ~ 50 DEG C temperature, after filtration or centrifugation remove colloid, then drying obtains giving off a strong fragrance rapeseed oil finished product.
CN201510049752.1A 2015-01-30 2015-01-30 Method for degumming fragrant rap oil through enzymic process Pending CN104651040A (en)

Priority Applications (1)

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CN201510049752.1A CN104651040A (en) 2015-01-30 2015-01-30 Method for degumming fragrant rap oil through enzymic process

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154222A (en) * 2015-07-31 2015-12-16 崇州市白头甘泉工业油脂厂 Phosphatide removal method for grease
CN105462684A (en) * 2015-11-26 2016-04-06 中粮粮油工业(黄冈)有限公司 Strong-flavor oil enzymatic-process degumming process
CN105670768A (en) * 2016-01-20 2016-06-15 和县绿源油脂有限公司 Aromatic compound bean oil and production process thereof
CN105950289A (en) * 2016-07-12 2016-09-21 中纺粮油（东莞）有限公司 Enzymatic degumming method for soybean crude oil
CN106190538A (en) * 2016-08-31 2016-12-07 东台市苏北油脂有限公司 The processing technique of Luzhou-flavor Oleum Brassicae campestris
CN108085131A (en) * 2017-12-25 2018-05-29 无锡德合食品科技有限公司 Cryogenic technique integrates the method for preparing high-quality rapeseed oil
CN111349514A (en) * 2020-03-30 2020-06-30 益海嘉里(岳阳)粮油工业有限公司 Method for degumming and refining rapeseed oil based on deep acid method
CN112899074A (en) * 2021-01-21 2021-06-04 宿迁市江南大学产业技术研究院 Method for degumming sesame oil
CN114369499A (en) * 2021-12-15 2022-04-19 郑州远洋油脂工程技术有限公司 Deep enzymatic degumming device and process
CN114574281A (en) * 2021-12-06 2022-06-03 中粮营养健康研究院有限公司 Enzymatic degumming method for strong-flavor oil

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CN101323815A (en) * 2008-07-25 2008-12-17 国家粮食储备局西安油脂科学研究设计院 Novel method for degumming of rape oil by using mixing enzyme preparation
CN102399627A (en) * 2011-09-26 2012-04-04 华南理工大学 Improved vegetable oil enzymatic degumming method
CN102504946A (en) * 2011-11-04 2012-06-20 福建春辉生物工程有限公司 Method for refining camellia seed crude oil beneficial to individuals with hypertension, hyperglycemia, hyperlipidemia
CN102586011A (en) * 2011-12-01 2012-07-18 华中农业大学 Method for degumming vegetable fat by using phosphatidase A2
CN102776066A (en) * 2012-07-24 2012-11-14 东北农业大学 Degumming method through immobilized phospholipase A2

Patent Citations (5)

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CN101323815A (en) * 2008-07-25 2008-12-17 国家粮食储备局西安油脂科学研究设计院 Novel method for degumming of rape oil by using mixing enzyme preparation
CN102399627A (en) * 2011-09-26 2012-04-04 华南理工大学 Improved vegetable oil enzymatic degumming method
CN102504946A (en) * 2011-11-04 2012-06-20 福建春辉生物工程有限公司 Method for refining camellia seed crude oil beneficial to individuals with hypertension, hyperglycemia, hyperlipidemia
CN102586011A (en) * 2011-12-01 2012-07-18 华中农业大学 Method for degumming vegetable fat by using phosphatidase A2
CN102776066A (en) * 2012-07-24 2012-11-14 东北农业大学 Degumming method through immobilized phospholipase A2

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154222A (en) * 2015-07-31 2015-12-16 崇州市白头甘泉工业油脂厂 Phosphatide removal method for grease
CN105462684A (en) * 2015-11-26 2016-04-06 中粮粮油工业(黄冈)有限公司 Strong-flavor oil enzymatic-process degumming process
CN105670768A (en) * 2016-01-20 2016-06-15 和县绿源油脂有限公司 Aromatic compound bean oil and production process thereof
CN105950289A (en) * 2016-07-12 2016-09-21 中纺粮油（东莞）有限公司 Enzymatic degumming method for soybean crude oil
CN106190538A (en) * 2016-08-31 2016-12-07 东台市苏北油脂有限公司 The processing technique of Luzhou-flavor Oleum Brassicae campestris
CN108085131A (en) * 2017-12-25 2018-05-29 无锡德合食品科技有限公司 Cryogenic technique integrates the method for preparing high-quality rapeseed oil
CN111349514A (en) * 2020-03-30 2020-06-30 益海嘉里(岳阳)粮油工业有限公司 Method for degumming and refining rapeseed oil based on deep acid method
CN112899074A (en) * 2021-01-21 2021-06-04 宿迁市江南大学产业技术研究院 Method for degumming sesame oil
CN114574281A (en) * 2021-12-06 2022-06-03 中粮营养健康研究院有限公司 Enzymatic degumming method for strong-flavor oil
CN114369499A (en) * 2021-12-15 2022-04-19 郑州远洋油脂工程技术有限公司 Deep enzymatic degumming device and process

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Application publication date: 20150527