CN104644611A - Method for preparing microcapsules - Google Patents

Abstract

The invention discloses a method for preparing microcapsules, belonging to the field of food and medicament capsule wall materials. Provided is a method for preparing microcapsules by adopting dry yeast cells as a capsule wall material without special culture. The method is used for preparing microcapsules by using dry yeast cells as a capsule wall material and using clove oil as a capsule core. By adopting the method for preparing the microcapsules, a special culture method is not needed; the adopted yeast does not have bioactivity, so that waste yeast coming from various sources and having no activity can be used as a raw material; and the prepared microcapsules have an antibacterial effect and certain sustained-release effect.

Description

A kind of microcapsule preparation method

Technical field

The invention belongs to food and medicine cyst material preparation field.

Background technology

Microcapsule technology room utilizes macromolecular compound that is natural or synthesis to prepare microcapsule as filmogen.The material being enclosed in microcapsule inside is called capsule-core, and the outside film formed by filmogen is called cyst wall.By micro encapsulation, core material can be made from the impact of external environment condition, reduce volatility, improve stability, and micro encapsulation also have shielding abnormal flavour, reduce the advantage such as toxicity and Co ntrolled release.Microcapsule technology brings breakthrough progress to food industry and medical industry, and the preparation of microcapsule adopts chemical method, physical method and physico-chemical process three class usually.But generally there is preparation process complexity in these methods, controlled condition precision prescribed is high, cost is high, constant product quality is poor, particularly suitable cyst material select the limitation such as difficulty.20 century 70s, technical staff develops and utilizes yeast cells to prepare the technology of microcapsule as natural cyst material, due to yeast cells feature, is desirable cyst material.

Compared with traditional microcapsule preparation method, be that cyst material is prepared microcapsule and had following advantage with yeast cells: in preparation process, do not need or seldom introduce other chemical reagent, the problem not having dissolvent residual or remove, being applicable to the coated of medicine and food additive; Yeast raw material sources are extensive, cheap; The microcapsule product size obtained is homogeneous, nontoxic, biocompatibility is good, easily biological-degradable.But adopt and prepare microcapsule in this way, generally have particular/special requirement to used yeast: as yeast need make fat content in cell reach more than 40% through specific culture condition; In microcapsule preparation process, yeast cells need keep biological activity, and normally use with the form of wet thallus, cell water content is high, bond between yeast cells in bulk, bad dispersibility, the hydration shell of cell surface can affect fat-soluble capsule-core and permeate in yeast cells, and follow-up drying, granulation operations are also more difficult.

Flos Caryophylli is a kind of rare spice, the Oleum Caryophylli extracted from Flos Caryophylli alabastrum, main component is that eugenol, β-Dianthus chinensis are rare etc., stronger inhibitory action is had to the polluted bacteria that food is common, therefore can as antiseptics for natural food, but Oleum Caryophylli volatility is comparatively strong, be subject to air, illumination effect and oxidation deterioration.

Summary of the invention

The object of this invention is to provide one not need through specific culture, adopt dry yeast cell to prepare the method for microcapsule as cyst material.

The present invention is achieved by the following technical programs: a kind of microcapsule preparation method, comprises preparation two step of the preparation of Oleum Caryophylli, clove oil microcapsules;

The preparation process of described Oleum Caryophylli is, using 40 ~ 60 order Flos Caryophylli alabastrum powder as raw material, at 70 DEG C, static extracting 30min, then dynamic extraction under same temperature, pressure under 10MPa, and CO ₂flow is 1.5L/min, CO ₂total consumption is 120L, and the Oleum Caryophylli repeatedly extracted is merged mixing;

The preparation process of described clove oil microcapsules is, precision takes the dry yeast of appropriate deactivation, puts into ground triangular flask, by the core of 1:1 than the Oleum Caryophylli adding a certain amount of preparation, thermostatic water bath vibrator is put into after adding a cover, after 70 DEG C of constant temperature constant speed concussion 9h, obtain microcapsule, incline and, vacuum filtration also reclaims the quintessence oil do not wrapped into, absolute ethanol washing, 30 DEG C of vacuum drying 20min, obtain microcapsule.

The present invention has following beneficial effect:

Adopt the method to prepare microcapsule, do not need through specific culture method, used yeast does not have biological activity, and what be convenient to utilize various source does not have activated waste yeast as raw material, and the microcapsule of preparation has fungistatic effect, and has certain slow release effect.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention will be further described.

Specific embodiment: preparation process of the present invention comprises the preparation of Oleum Caryophylli, preparation two step of clove oil microcapsules;

The preparation process of described Oleum Caryophylli is, using 40 ~ 60 order Flos Caryophylli alabastrum powder as raw material, at 70 DEG C, static extracting 30min, then dynamic extraction under same temperature, pressure under 10MPa, and CO ₂flow is 1.5L/min, CO ₂total consumption is 120L, and the Oleum Caryophylli repeatedly extracted is merged mixing;

The preparation process of described clove oil microcapsules is, precision takes the dry yeast of appropriate deactivation, puts into ground triangular flask, by the core of 1:1 than the Oleum Caryophylli adding a certain amount of preparation, thermostatic water bath vibrator is put into after adding a cover, after 70 DEG C of constant temperature constant speed concussion 9h, obtain microcapsule, incline and, vacuum filtration also reclaims the quintessence oil do not wrapped into, absolute ethanol washing, 30 DEG C of vacuum drying 20min, obtain microcapsule.

By microscope and electron microscopic observation, clove oil microcapsules is spherical in rule, particle diameter is between 2.O ~ 4.0 μm, the existence of glittering oil droplet can be observed in Cytoplasm, in microcapsule, Oleum Caryophylli embedding rate can reach 41.26%, heat 20h under 100 DEG C of high temperature after, Oleum Caryophylli volatility is 58.29%, and residual mass only has 41.71%; Oleum Caryophylli volatility after micro encapsulation only has 15.04%, declines 43.25%, well below the rate of volatilization of Oleum Caryophylli, therefore micro encapsulation significantly can reduce the rate of volatilization of Oleum Caryophylli, more be conducive to its effect of permanent performance, fungistatic effect higher than Oleum Caryophylli, and has slow release effect.

Above content is the further description done the present invention in conjunction with concrete embodiment, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.

Claims (1)

1. a microcapsule preparation method, is characterized in that: preparation two step comprising the preparation of Oleum Caryophylli, clove oil microcapsules;

The preparation process of described Oleum Caryophylli is, using 40 ~ 60 order Flos Caryophylli alabastrum powder as raw material, at 70 DEG C, static extracting 30min, then dynamic extraction under same temperature, pressure under 10MPa, and CO ₂flow is 1.5L/min, CO ₂total consumption is 120L, and the Oleum Caryophylli repeatedly extracted is merged mixing;

The preparation process of described clove oil microcapsules is, precision takes the dry yeast of appropriate deactivation, puts into ground triangular flask, by the core of 1:1 than the Oleum Caryophylli adding a certain amount of preparation, thermostatic water bath vibrator is put into after adding a cover, after 70 DEG C of constant temperature constant speed concussion 9h, obtain microcapsule, incline and, vacuum filtration also reclaims the quintessence oil do not wrapped into, absolute ethanol washing, 30 DEG C of vacuum drying 20min, obtain microcapsule.