CN104622931A - Application of eight-component pill in preparation of medicine for preventing or treating cerebral ischemia and its complication - Google Patents
Application of eight-component pill in preparation of medicine for preventing or treating cerebral ischemia and its complication Download PDFInfo
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- CN104622931A CN104622931A CN201510019151.6A CN201510019151A CN104622931A CN 104622931 A CN104622931 A CN 104622931A CN 201510019151 A CN201510019151 A CN 201510019151A CN 104622931 A CN104622931 A CN 104622931A
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Abstract
The invention belongs to the field of medicines, and in particular relates to application of an eight-component pill in preparation of a medicine for preventing or treating cerebral ischemia and its complication. The complication of cerebral ischemia includes cerebral infarction or memory impairment caused by cerebral ischemia. The eight-component pill is mainly used for reducing peroxide content of nitric oxide and nitric oxide synthase in cerebral hippocampus and protecting nerve cell of cerebral hippocampus, so as to achieve the effect of treating cerebral ischemia; the MDA content in blood serum is decreased, and thus the damage of free radical to brain is reduced, the area of cerebral infarction is reduced, and as a result, the effect of treating cerebral infarction caused by cerebral ischemia is achieved; the pharmacological experiment shows that the eight-component pill has the effect of preventing memory impairment caused by cerebral ischemia. The eight-component pill has an obvious effect of preventing or treating cerebral ischemia, cerebral infarction or memory impairment caused by cerebral ischemia, is free of side effects, and has obvious clinical popularization value.
Description
Technical field
The invention belongs to field of medicaments, particularly relate to the purposes of pill of Eight Treasures in preparation prevention or treatment cerebral ischemia and complication medicine thereof.
Background technology
Cerebral ischemia is medically called apoplexy, refers to the blood flow interrupting or reduce in the tremulous pulse of supporting brain.Research in recent years shows, cerebral ischemia not only occurs neuronal cell death. Late-onset neuronal death simultaneously, and Delayed onset or selecting cell death are mainly realized by apoptosis, full brain or local cerebral infarction all can active cell apoptotic processes.Cerebral ischemia is harm humans life and healthy frequently-occurring disease, has the advantages that sickness rate is high, disability rate is high, case fatality rate is high and relapse rate is high.At present the several key factors close with relationship with apoptosis are mainly concentrated on to the study mechanism of the medicine for the treatment of cerebral ischemia, as the impact of gene expression after the activation of oxygen-derived free radicals, nitric oxide, toxicity of excitatory amino acid, calcium overload factor, transcription signal, ischemia, but not yet work out effective medicine at present, therefore, finding novel effective drugs for cerebral ischemia therapy is current study hotspot.
Pill of Eight Treasures is the preparation be made up of Calculus Bovis, Fel Serpentis, Cornu Saigae Tataricae, Margarita, Radix Notoginseng, Moschus etc., and have antiinflammatory, refreshing and detoxicating, reducing swelling and alleviating pain generates heat caused by damp-heat accumulation, jaundice, yellowish or reddish urine, nausea and vomiting, indigestion and loss of appetite, hypochondriac pain abdominal distention
, yellowish fur or thick greasy extra dry white wine, or urethra scorching hot twinge, lower abdominal distention pain caused by damp invasion of lower energizer, and spreading venereal diseases toxicity hepatitis,
acutethe therapeutical effect such as cholecystitis, the infection of acute urinary system, curing mainly function is heat-clearing and toxic substances removing, cooling blood and removing stasis, reducing swelling and alleviating pain.For acute and chronic hepatitis, carbuncle furuncle, innominate toxic swelling, traumatic injury and various inflammation caused by pyretic toxicity blood stasis.Show through pharmacological research, pill of Eight Treasures has the effects such as antiinflammatory, refreshing and detoxicating, reducing swelling and alleviating pain, to the disease such as pain, heating that acute hepatitis, ophthalmia, otitis and all inflammation cause, has good curative effect; In addition, obvious inhibitory action is had to digestion system cancer cell.At present, the aspects such as pill of Eight Treasures cerebral ischemia, the cerebral infarction caused due to cerebral ischemia, the impaired memory function that causes due to cerebral ischemia yet there are no relevant report.
Summary of the invention
In order to solve prior art treatment cerebral ischemia and complication there is no the defect of effective medicine, the object of the invention is that the purposes of open pill of Eight Treasures in preparation prevention or treatment cerebral ischemia and complication medicine thereof is to solve the above problems.
Pill of Eight Treasures has the effects such as antiinflammatory, refreshing and detoxicating, reducing swelling and alleviating pain, has good curative effect to the disease such as pain, heating that acute hepatitis, ophthalmia, otitis and all inflammation cause; In addition, obvious inhibitory action is had to digestion system cancer cell.The invention discloses the purposes of pill of Eight Treasures in preparation prevention or treatment cerebral ischemia and complication medicine thereof, described complication is the impaired memory function that the cerebral infarction that causes of cerebral ischemia and cerebral ischemia cause, pill of Eight Treasures is mainly by reducing the peroxide content of nitric oxide in cerebral hippocampus and nitricoxide synthase, protection hippocampal neurons in mouse, reach the effect of prevention or treatment cerebral ischemia, pill of Eight Treasures is by reducing Content of MDA, reduce the damage of radical pair brain, thus reduce the area of cerebral infarction plug, reach the effect of the cerebral infarction preventing or treat cerebral ischemia to cause, realize confirming through drug effect simultaneously, pill of Eight Treasures has the effect of the impaired memory function that protection cerebral ischemia causes.Pill of Eight Treasures is oral formulations, and its people is with dosage 1 mg/kgd-500 mg/kgd, and preferred people's dosage is 10mg/kgd-400 mg/kgd.In the impaired memory function that pill of Eight Treasures of the present invention causes in the cerebral infarction preparing prevention and therapy cerebral ischemia, cerebral ischemia causes, cerebral ischemia, therapeutic effect is remarkable, has no side effect, has significant clinical generalization value.
Relative to prior art, the invention has the advantages that: in the impaired memory function that pill of Eight Treasures of the present invention causes in the cerebral infarction preparing prevention and therapy cerebral ischemia, cerebral ischemia causes, cerebral ischemia, therapeutic effect is remarkable, have no side effect, effectively alleviate the misery of patient, there is significant clinical generalization value.
Specific embodiment
embodiment 1, pill of Eight Treasures are on the impact of cerebral ischemic model rat hippocampus nitric oxide and nitricoxide synthase
This test adopts nimodipine as positive control medicine, and it is a kind of Ca
2+channel blocker, by effectively stoping Ca
2+enter in cell, suppress smooth muscle contraction, reach the object removing vasospasm.Zoopery proves, nimodipine is many by force far beyond the effect of other position tremulous pulsies of whole body to arteriocerebral effect, and because it has very high fat-soluble feature, easily through blood brain barrier.In addition, optionally act on cerebrovascular smooth muscle, expansion of cerebral vascular, increase cerebral blood flow, obviously reduce the ischemic brain injury that vasospasm causes.
1, materials and methods
1.1 animal groupings
The healthy male SD rat 72 of SPF level, body weight 280 ~ 320 g, is provided by Guangdong Medical Lab Animal Center.Modeling is after 6 weeks, and SD rat is divided into 6 groups at random, Normal group, model group, nimodipine group, pill of Eight Treasures one group, pill of Eight Treasures two groups and pill of Eight Treasures three groups, often organizes 12.After modeling, the 7th week Normal group and model group SD rat give isopyknic solvent gavage respectively, nimodipine group SD rat gives 10mg/ (kgd), pill of Eight Treasures one group of SD rat dosage 50mg/ (kgd) (gauge according to pill of Eight Treasures powder), pill of Eight Treasures two groups of SD rat dosages 75 mg/ (kgd) (gauge according to pill of Eight Treasures powder), pill of Eight Treasures three groups of SD rat dosages are 100mg/ (kgd) (gauge according to pill of Eight Treasures powder), successive administration 3 weeks.
1.2 reagent and instrument
The pill of Eight Treasures capsule (the accurate word Z10940006 of traditional Chinese medicines) that pill of Eight Treasures adopts the Xiamen pharmaceutical factory of traditional Chinese medicine to produce;
Nimodipine: purchased from Kangyuan Pharmaceutical Co., Ltd., Jiangsu Prov;
NO, NOS test kit: build up Bioengineering Research Institute purchased from Nanjing;
Primary antibodie nNOS, iNOS, eNOS and t3 actin antibodies: Beijing Bo Aosen Bioisystech Co., Ltd; Goat-anti rabbit Ig-G: Beijing Ding Guo Bioisystech Co., Ltd.
DYCZ-24D type vertical slab electrophoresis groove, Liuyi Instruments Plant, Beijing;
JS-250 electrophresis apparatus, Shanghai Peiqing Science Co., Ltd;
TS-2000A decolorization swinging table, its woods Bel instrument manufacturing company limited of Jiangsu;
MDF-U333 cryogenic refrigerator, SANY0; Precisa XS125 electronic balance;
UV2550 spectrophotometer, Japanese Shimadzu.
The preparation of 1.3 cerebral ischemia SD rat models
SD rat pre-operative anxiety 12 h, taboo water 4 h, with 10% chloral hydrate (0.30 ml/100 g) intraperitoneal injection of anesthesia, lie on the back and be fixed on wooden operating-table.Throat portion preserved skin, after ethanol, iodine disinfection, along veutro neck medisection, blunt separation subcutaneous fat, sternohyoid and sternocleidomastoid muscle successively, be separated respectively again and expose left and right side common carotid artery and trachea, cut off from centre after proximal part and the dual ligation of distal end, then layer-by-layer suture muscle, skin.Normal group SD rat, with method row throat incision of skin, is separated subcutaneous fat.
The mensuration of 1.4 NO content and NOS activity
After modeling the 10th week, each group SD rat broken end got brain, removes olfactory bulb, cerebellum, takes out cerebral hippocampus and weigh, add 9 times of cold salines, electric homogenate machine grinds, and makes 10% homogenate, 3000 r/min, centrifugal 10 min, get supernatant, measure NO content and NOS activity by test kit description.
The albumen getting equivalent after each group of SD rat fresh brain tissue Hippocampus extract proteins carries out SDS-PAGE separation, electrotransfer is to nitrocellulose filter, room temperature closes 30 min, nNOS, iNOS, eNOS and t3 actin primary antibodie 500 is doubly diluted in confining liquid, 4 DEG C of reactions spend the night after incubated at room 2 h.After abundant rinsing, two of horseradish peroxidase-labeled anti-goat anti-rabbit iggs 1500 are doubly diluted in confining liquid, incubated at room 1 h.Film is placed in DAB nitrite ion, development, fixing, exposure.Utilize the gray value of Quantity one software analysis band.
1.5 statistical method
Adopt SPSS 12.0 statistical software, data represent with x ± s, and group is asked and compared employing variance analysis, and the neat person of variance adopts LSD to check, and heterogeneity of variance person adopts Tamhane ' S to check, and P<0.05 is that difference has statistical significance.
2 results
The comparison of 2.1 each group SD rat hippocampus NO content and NOS activity
As shown in table 1, compare with Normal group, model group SD rat hippocampus NO content and the active obviously rising (P<0.01) of NOS; Compare with model group, nimodipine group and pill of Eight Treasures each dosage group SD rat hippocampus NO content and NOS is active obviously reduces (P<0.05); Compared with positive control drug nimodipine, pill of Eight Treasures each dosage group SD rat hippocampus NO content and NOS activity all significantly reduce (P<0.05), and wherein pill of Eight Treasures two groups has pole significant difference (P<0.01) compared with Treated with Nimodipine group.
Table 1 each administration group SD rat hippocampus NO content and NOS expression activitiy
| Group | Sample size (n) | NO（μmol/g） | NOS（U/mg） |
| Normal group | 12 | 2.38±1.00 | 1.36±0.32 |
| Model group | 12 | 5.70±0.90 ** | 3.46±0.42 ** |
| Nimodipine group | 12 | 4.82±0.98 ￥ | 2.62±0.52 ￥ |
| Pill of Eight Treasures one group | 12 | 2.98±0.76 ￥￥# | 1.84±0.48 ￥￥# |
| Pill of Eight Treasures two groups | 12 | 2.52±0.86 ￥￥## | 1.48±0.42 ￥￥## |
| Pill of Eight Treasures three groups | 12 | 3.18±0.96 ￥￥# | 2.08±0.44 ￥￥# |
Compared with Normal group,
*p < 0.01; Compared with model group,
$p < 0.05,
$$p < 0.01;
Compared with nimodipine group,
#p < 0.05,
##p < 0.01.
The comparison of 2.2 each group SD rat hippocampus, 3 kinds of NOS subtype expression
With shown in table 2, compare with Normal group, model group SD rat hippocampus nNOS and iNOS expresses and obviously raises, and eNOS expresses and obviously reduces (P<0.01); Compare with model group, nimodipine group and pill of Eight Treasures two groups of SD rat hippocampus nNOS and iNOS express and obviously reduce, and eNOS expresses and obviously raises (P<0.05); Compared with positive control drug nimodipine, pill of Eight Treasures each administration group SD rat hippocampus nNOS and iNOS expresses and obviously reduces, eNOS expresses and obviously raises (P<0.05), and wherein pill of Eight Treasures two groups has pole significant difference (P<0.01) compared with Treated with Nimodipine group.
The comparison of table 2 each administration group SD rat hippocampus NOS 3 kinds of subtype expression
| Group | Sample size (n) | nNOS（mol/g） | iNOS（U/mg） | eNOS |
| Normal group | 12 | 0.48±0.12 | 1.06±0.32 | 1.90±0.28 |
| Model group | 12 | 1.40±0.32 ** | 3.30±0.38 ** | 1.02±0.36 ** |
| Nimodipine group | 12 | 0.90±0.24 ￥ | 2.40±0.54 ￥ | 1.20±0.32 ￥ |
| Pill of Eight Treasures one group | 12 | 0.60±0.28 ￥￥# | 1.90±0.44 ￥￥# | 1.50±0.18 ￥￥# |
| Pill of Eight Treasures two groups | 12 | 0.58±0.22 ￥￥## | 1.48±0.38 ￥￥## | 1.66±0.18 ￥￥## |
| Pill of Eight Treasures three groups | 12 | 0.74±0.26 ￥# | 2.20±0.42 ￥# | 1.28±0.28 ￥# |
Compared with Normal group,
*p < 0.01; Compared with model group,
$p < 0.05,
$$p < 0.01;
Compared with nimodipine group,
#p < 0.05,
##p < 0.01.
embodiment 2,pill of Eight Treasures is to the therapeutical effect of the cerebral infarction caused due to cerebral ischemia
1. experiment material
The laboratory animal adopted in this experiment is male SD rat, and body weight is 280-320g, raises under room temperature under 12h circadian rhythm, free diet.Thered is provided by Guangdong Medical Lab Animal Center.The pill of Eight Treasures capsule (the accurate word Z10940006 of traditional Chinese medicines) that test sample in this experiment adopts the Xiamen pharmaceutical factory of traditional Chinese medicine to produce; Reference substance is pure water.
The associated materials that this experiment uses and reagent as follows: MDA test kit (purchased from Shanghai history is auspicious can bio tech ltd), 20% chloral hydrate, povidone iodine, cresyl viollet, hydrogen peroxide, ethanol, dimethylbenzene, 4% paraformaldehyde, normal saline are laboratory common agents.This experiment key instrument and equipment: rat head holder, electrocoagulator, 721 type visible spectrophotometers, pathological tissue float and dry processing instrument, paraffin slicing machine, microscope digital camera are pharmacological evaluation room common instrument.
2. experimental technique
2.1 animal groupings: laboratory animal is divided into 4 groups at random: model group, sham operated rats, pill of Eight Treasures one group of SD rat dosage 50mg/ (kgd) (gauge according to pill of Eight Treasures powder), pill of Eight Treasures two groups of SD rat dosages 75 mg/ (kgd) (gauge according to pill of Eight Treasures powder), pill of Eight Treasures three groups of SD rat dosages are 100mg/ (kgd) (gauge according to pill of Eight Treasures powder), often organize 12.
The foundation of 2.2 rat cerebral ischemia models: laboratory animal, with after 20% chloral hydrate (300-350 mg/kg) intraperitoneal injection of anesthesia, is separated bilateral common carotid arteries and coagulation vertebral artery.Perform the operation second day animal ligation bilateral common carotid arteries under waking state, ischemia is decontroled bulldog clamp after 15 minutes and is filled with.Keep its rectal temperature during ischemia between 36.5-37.5 DEG C.The reliability of ischemia model is judged with the performance of ischemic animal sign.Rat cerebral ischemia model standard of perfection is as follows: before ischemia, rat is in complete waking state, and its vital sign is normal.After ischemia, in 30-60 second, namely rat loses consciousness and mobility, and righting reflex loss, presents opisthotonus, simultaneously analgesis, bilateral pupil dilation, stimulates close eyelid areflexia, eyeball color greyish white (being normally cerise) to high light.Sham operated rats process is with each administration group of pill of Eight Treasures, but not ligation bilateral common carotid arteries.The rat cerebral ischemia model modeling success rate adopting the method to prepare in this experiment reaches 94.5%.
2.3 route of administration and time: pill of Eight Treasures one group, pill of Eight Treasures two groups and pill of Eight Treasures three groups all after ischemia/reperfusion the 8th hour by the dosed administration of specifying once, first administration after 16 hours with same dose administration again.Sham operated rats and model group give the distilled water of same volume, and dosage regimen is with pill of Eight Treasures administration group.
, laboratory observation and index determining
3.1 Content of MDA measure: put to death rat after pill of Eight Treasures each administration group and last 1 the administration 8h of model group, get blood in the quick chambers of the heart, centrifuging and taking serum ,-20 DEG C frozen; Adopt thiobarbituricacidα-(TBA) colorimetric method for determining; The strict by specification of operational approach carries out.
3.2 cerebral infarction volumes are measured: through the brain sheet of TTC dyeing, take pictures with digital camera, and application high-definition color pathology picture and text report analytical system measures each district brain infarction area.According to formula: Infarction volume=［ left side hemisphere volume-(right side hemisphere volume-pale district volume) ］/full brain volume, calculates Infarction volume percentage ratio.
3.3 Histological methods: rat is after lumbar injection chloral hydrate anesthesia, open left breast and expose heart, syringe needle is inserted aorta from the apex of the heart through left ventricle, first be about the quick lavation of 100ml with about 38 DEG C normal saline, then the paraformaldehyde 250-300ml of ice-cold 4% is poured into again, filled with in 30 minutes, the rat broken end after perfusion is got brain and it is directly fixed 12 hours with after the paraformaldehyde of 4%.Running water, the washing time same set time.Then 70%(4h), 80% (4h), 90% (4-12h), 95% (2h × 2 time), 100% (2h × 2 time) gradient alcohol dehydration, dimethylbenzene transparent (1.5h), 60 DEG C of waxdips (1h × 3 time).Take out embedding, 4 DEG C of preservations.Paraffin slicing machine is cut into slices, thick 5 μm.In 45 DEG C of warm water, paster is on the microscope slide of gelatin process, and 40 DEG C of bakings are spent the night.Cut into slices through three dimethylbenzene dewaxings, 100%, 90%, 70% graded ethanol immersion, dye 0.1% cresyl viollet (Cresyl violet) 10-20 minute, according to a conventional method 70%, 80%, 95%, 100% graded ethanol color separation, light Microscopic observation neuron purple and background substantially colourless till.Gradient alcohol dehydration, dimethylbenzene is transparent, after resinene mounting, om observation.At survival and the apoptosis of high power Microscopic observation neuronal cell.There is clearly nucleus, painted the counting and survive neuronal cell well of cell space; Core shrinkage, deformed is the neuronal cell of apoptosis.
date processing:
Experimental result application SPSS12.0 software is analyzed, and each Sets of Measurement Data represents with mean ± standard deviation (mean ± SD).Between each group, the significance of data compares employing t inspection; Assay judges, is that difference has significance with p<0.05.
experimental result
5.1 Content of MDA measurement results: as shown in table 3, model group MDA content is significantly higher than sham operated rats, shows that before and after cerebral ischemia modeling, MDA content has significant difference.Pill of Eight Treasures one group, pill of Eight Treasures two groups and pill of Eight Treasures three groups, all there is significant difference (P<0.05) with model group MDA content in its Content of MDA, wherein pill of Eight Treasures two groups and model group exist pole significant difference (P<0.01).
5.2 cerebral infarction volume measurement results: as shown in table 3, sham operated rats is without cerebral infarction.Pill of Eight Treasures one group, pill of Eight Treasures two groups have significant difference (P<0.05) with the cerebral infarction volume of pill of Eight Treasures three groups compared with model group cerebral infarction volume, and wherein pill of Eight Treasures two groups has pole significant difference (P<0.01) compared with model group.
Between table 3 rat each administration group, Content of MDA and cerebral infarction volume compare
| Group | Content of MDA | Cerebral infarction volume |
| Sham operated rats | 2.56±3.0 | —— |
| Model group | 8.12±0.64 | 18.02±2.28 |
| Pill of Eight Treasures one group | 5.48±0.54 * | 15.22±2.30 * |
| Pill of Eight Treasures two groups | 4.78±0.62 ** | 13.98±1.92 ** |
| Pill of Eight Treasures three groups | 5.52±0.44 * | 15.72±2.26 * |
Compare with model group,
*p<0.05,
*p<0.01.
The experimental result display of this experiment, pill of Eight Treasures not only significantly can reduce the Content of MDA of rat cerebral ischemia model, reduce the damage of radical pair rat brain, and the cerebral infarct size of rats with cerebral ischemia can be reduced, visible pill of Eight Treasures has significant therapeutic effect to the ischemic brain injury disease cerebral infarction that particularly cerebral ischemia and cerebral ischemia cause.
embodiment 3,pill of Eight Treasures is to the therapeutical effect of the impaired memory function that cerebral ischemia causes:
1 material
The pill of Eight Treasures capsule (the accurate word Z10940006 of traditional Chinese medicines) that pill of Eight Treasures adopts the Xiamen pharmaceutical factory of traditional Chinese medicine to produce, the laboratory animal adopted in this experiment is SD rat, and keeping away dark auto testing instrument is Chengdu TME Technology Co., Ltd.'s product.
2 experimental techniques
2.1 the foundation of experimental group: get SD rat and be divided into following drug treatment group at random, often organize 12.Each treatment group gives following medicine respectively:
Normal group: gavage gives the normal saline of same volume;
Model group: gavage gives the normal saline of same volume;
Pill of Eight Treasures one group: gavage gives pill of Eight Treasures capsule 50mg/ (kgd) (gauge according to pill of Eight Treasures powder);
Pill of Eight Treasures two groups: gavage gives pill of Eight Treasures capsule 75 mg/ (kgd) (gauge according to pill of Eight Treasures powder);
Pill of Eight Treasures three groups: gavage gives pill of Eight Treasures capsule 100mg/ (kgd) (gauge according to pill of Eight Treasures powder);
2.2 step-through test behavioristicss are detected
Keep away active box point light and shade two Room of dark auto testing instrument, have a hole between two Room, at the bottom of case, pass to copper grid.Before formal experiment, each group of SD rat is trained, SD rat head is carried Fang Renming room, hole. first conform 2min, and lead to 36V electric current then to darkroom copper grid, SD rat is subject to electric shock and namely runs away to bright room after entering darkroom, copper grid continue energising 5min, and this is training process.Carry out the test of memory of SD rat after 24h, record SD rat enters the time (keeping away dark incubation period) in darkroom for the first time, if enter darkroom not yet in SD rat 5min.Its incubation period, 300s made by meter.
3 statistical methods
Experimental data represents with ± s, carries out statistical analysis with SPSS11.5 software kit, adopts ANOVA and LSD ' S posthoc test to carry out statistical analysis, indicates significant difference with P<0.05.
4 experimental results
The impact of pill of Eight Treasures each administration group on SD rat step-through test is as shown in table 3.
Table 4 pill of Eight Treasures keeps away dark preclinical impact (± s) to SD rat
| Group | n | Keep away dark incubation period (s) |
| Normal group | 12 | 23.02±5.52 |
| Model group | 12 | 107.02±9.08 ## |
| Pill of Eight Treasures one group | 12 | 52.32±8.20* |
| Pill of Eight Treasures two groups | 12 | 47.22±6.40** |
| Pill of Eight Treasures three groups | 12 | 63.02±8.58* |
Compared with normal group,
##p < 0.01; Compared with model group, * P < 0.05, * * P < 0.01;
As can be seen from Table 4, pill of Eight Treasures each administration group keeps away dark significant prolongation incubation period (P < 0.01) relative to SD rat, shows that the SD rat of the impaired memory function that pill of Eight Treasures each administration group causes relative to cerebral ischemia has significant prevention and therapy effect.Be embodied in:
1) the SD rat of pill of Eight Treasures each administration group is kept away and has significant difference compared with model group dark incubation period, all significantly shorten SD rat and keep away dark incubation period, there is significant difference, pill of Eight Treasures has significant therapeutic effect to the impaired memory function that cerebral ischemia causes.
2) the SD rat escape latency of pill of Eight Treasures each administration group is variant, best by the effect of the known pill of Eight Treasures of table 4 data two groups, this shows that the treatment of the pill of Eight Treasures of variable concentrations to the impaired memory function that cerebral ischemia causes has diversity, and wherein the impaired memory function therapeutic effect that causes of the cerebral ischemia of pill of Eight Treasures to SD rat of 75 mg/kg/d is best.
Claims (5)
1. the purposes of pill of Eight Treasures in preparation prevention or treatment cerebral ischemia and complication medicine thereof.
2. purposes as claimed in claim 1, it is characterized in that, described complication is the cerebral infarction that cerebral ischemia causes.
3. purposes as claimed in claim 1, it is characterized in that, described complication is the impaired memory function that cerebral ischemia causes.
4. purposes as claimed in claim 1, it is characterized in that, pill of Eight Treasures is oral formulations, and its people is with dosage 1 mg/kgd-500 mg/kgd.
5. purposes as claimed in claim 4, it is characterized in that, pill of Eight Treasures is oral formulations, and its people's dosage is 10mg/kgd-400mg/kgd.
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