CN104622759A - Extracting method and application for adenium obesum leaf cell extract - Google Patents
Extracting method and application for adenium obesum leaf cell extract Download PDFInfo
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- CN104622759A CN104622759A CN201510074759.9A CN201510074759A CN104622759A CN 104622759 A CN104622759 A CN 104622759A CN 201510074759 A CN201510074759 A CN 201510074759A CN 104622759 A CN104622759 A CN 104622759A
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- rosae multiflorae
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Abstract
The invention discloses an extracting method for an adenium obesum leaf cell extract. The extracting method comprises the following steps: taking fresh adenium obesum leaves and crushing the fresh adenium obesum leaves; feeding raw material powder into a supercritical extraction kettle to perform supercritical CO2 extraction; taking and feeding an extracting mixture into a supercritical separation kettle to perform separation; adding separation products into distilled water to distill, and condensing and recycling steam to obtain the adenium obesum leaf cell extract. The extract is applied to a skin care product. Compared with the prior art, the extracting method can quickly extract much adenium obesum leaf cell extract on the premise of not damaging natural active ingredients of the adenium obesum, so that the yield is high, the impurities are few, solvent residues are avoided, and the natural characteristics of the extract are kept.
Description
Technical field
The present invention relates to technical field of plant extraction, be specifically related to desert Folium rosae multiflorae cell extract extracting method and by the application of this extract in skin care item.
Background technology
Desert Flos Rosae Multiflorae, has another name called Tian Bao flower, belongs to Apocynaceae, originates in south, East Africa to the Arabia Peninsula, is distributed in the north African countries such as Tunisia, Algeria, Morocco.
Desert Flos Rosae Multiflorae happiness high temperature drying and sunny environment, heat-resistant, do not resist cold, drought-resistant, avoid water temperature.Therefore, the sandy loam that its planting environment is calcareous with richness, draining is good is advisable.Desert rosaceous is Herba Cladoniae verticillatae seemingly, and rose is very gorgeous.Umbel 3 fifty percent clumps, magnificent like brocade, perpetual bloom is continuous.Because original producton location is close to desert and red desert Flos Rosae Multiflorae of gaining the name as Flos Rosae Rugosae.The leaf of desert Flos Rosae Multiflorae is single leaf alternate, obovate, the anxious point in top, long 8 centimetres ~ 10 centimetres, wide 2 centimetres ~ 4 centimetres, and keratin is glossy, outside of belly bottle green, back side celadon, Quan Yuan.
Show according to research; the flower elite of desert Flos Rosae Multiflorae has magical sun-proof function; include the trehalose composition of high concentration; ventilative water adipose membrane can be formed on skin cell surface; the water storage function of available protecting cell membrane and skin; skin is attacked from free radical, reduces tanned and generation that is skin ageing phenomenon.Have article to point out, the compound quintessence extracted from the leaf of desert Flos Rosae Multiflorae can help skin deeply lasting moisturizing, and the moisturizing potential exciting skin natural, resists the impact of ambient pressure and aging.
Seldom, active component contained in the Folium rosae multiflorae cell extract of desert has skin-moisturizing, skin, anti-ageing beauty functions of waiting for a long time for the current extraction for desert Folium rosae multiflorae cell extract and application.Therefore, set up effective extracting method, the research of its bioactive ingredients in anti-ageing beauty treatment of waiting for a long time can be promoted.
The method that prior art extracts desert Folium rosae multiflorae cell extract is steam distillation, specifically: pluck fresh, pure desert Folium rosae multiflorae, they are ground into particle diameter and are about 45 object material powders, therefrom get 500g material powder, join in 2000 ~ 3500ml distilled water, soak 30min ~ 40min, then thermal distillation is added with straight fire, steam containing material powder solvent components is steamed, what obtain after condensing reflux pipework condensation is Folium rosae multiflorae cell extract, condistillation 5 ~ 7 hours, the desert Folium rosae multiflorae cell extract finally obtained is about 0.15g, yield only only has 3/10000ths.Although above-mentioned technology has, equipment is simple, cost is low, advantage to environment and mankind's nonhazardous, there is the shortcomings such as yield is low, impurity content is higher.
Because the extractive content of current extracting method gained is extremely low, be thus difficult to desert Folium rosae multiflorae cell extract to be applied, the extracting method working out a kind of high yield pulp1 becomes the difficult problem applied desert Folium rosae multiflorae cell extract and must solve.
Summary of the invention
The object of the invention is to the defect overcoming above-mentioned present technology, the extracting method of a kind of desert Folium rosae multiflorae cell extract is provided, the method is under the prerequisite of active skull cap components not destroying desert Flos Rosae Multiflorae, a large amount of desert Folium rosae multiflorae cell extracts can be extracted rapidly, not only yield is high, and impurity is few, there is no dissolvent residual, keep the natural characteristic of extract.
Technical scheme of the present invention is: choose fresh desert Flos Rosae Multiflorae leaf, carry out supercritical CO after pulverizing
2extraction Bound moisture steam distillation, extracts desert Folium rosae multiflorae cell extract.Supercritical CO
2extraction has active component in no solvent residue, natural plants and to be not easily decomposed the advantage destroyed, and retains the characteristic of natural plants, combines the yield turn improving extract with vapor distillation.
The concrete technical scheme of the present invention is as follows:
An extracting method for desert Folium rosae multiflorae cell extract, is characterized in that: the leaf getting fresh desert Flos Rosae Multiflorae, pulverizes; Material powder is sent in supercritical extraction reactor and carries out supercritical CO
2extraction; Get in extraction mixture feeding supercritical separating still and be separated; Separated product adds in distilled water and distills, and steam condensing reclaims and obtains desert Folium rosae multiflorae cell extract.
Preferably, physical pulverization desert Flos Rosae Multiflorae leaf is to particle diameter≤40 object material powder.
Preferably, supercritical CO
2during extraction, CO
2flow velocity is 20 ~ 25kg/h, and extracting pressure is 20 ~ 25MPa, extraction temperature is extract at 35 DEG C, extraction time 1 ~ 3 hour.
Preferably, separating pressure when supercritical is separated is 8 ~ 12MPa, temperature is 40 DEG C, is separated 1 hour.
Preferably, the product that supercritical is separated is 1:(40 ~ 60 in solid-liquid ratio) ratio of g/L is added to the water and distills.
Preferably, the extracting method concrete steps of desert of the present invention Folium rosae multiflorae cell extract are as follows:
(1) select fresh, the complete and pure desert Flos Rosae Multiflorae leaf of high-quality, put into reducing mechanism after cleaning up and be ground into particle diameter≤40 object material powder;
(2) material powder is transported in supercritical extraction reactor, input CO in supercritical extraction reactor
2fluid, CO
2flow velocity is 20 ~ 25kg/h, extracting pressure is adjusted to 20 ~ 25MPa, extraction temperature is extract at 35 DEG C, extract 2 hours;
(3) take out extraction mixture from supercritical extraction reactor outlet, mixture is input in supercritical separating still, separating pressure is adjusted to 8 ~ 12MPa, temperature is 40 DEG C, be separated 1 hour;
(4) be that 1:50g/L is added to the water above separated product by solid-liquid ratio, straight fire adds thermal distillation 2 hours, and steam condensing reclaims and obtains desert Folium rosae multiflorae cell extract.
Inventor finds after deliberation, above-mentioned desert Folium rosae multiflorae cell extract is to no skin irritation, spread upon skin can make skin more add water profit, compact, there is skin-moisturizing, skin, speckle dispelling, antidotal effect, therefore, present invention also offers the application of desert Folium rosae multiflorae cell extract in skin care item.
Described skin care item are protective skin cream, facial film, facial cream, facial milk cleanser, cosmetic water, emulsion, essence, eye cream.
Described skin care item are spot-eliminating skin care product, Skin whitening care cosmetics, Moisturizer.
Compared with prior art, the invention has the beneficial effects as follows:
1, supercritical CO
2extraction has active component in no solvent residue, natural plants and is not easily decomposed and destroys and keep the advantage of its natural characteristic, and the present invention, on the basis of steam distillation, incorporates supercritical CO
2extraction, two kinds of methods combining get up, and not only yield is high to make the desert Folium rosae multiflorae cell extract that extracts, and impurity is few, and energy-conservation, quick;
2, the present invention extracts the desert Folium rosae multiflorae cell extract obtained and there is not heavy-metal residual problem, product safety;
3, the present invention adds to the desert Folium rosae multiflorae cell extract extracted in cosmetics, and dialogue rabbit skin carries out irritant test, finds this cosmetics nonirritant, and has fine freckle removing and whitening, the anti-aging beauty-care effects such as wrinkle removal skin care.
Detailed description of the invention
Below by specific embodiment, the present invention is described in further details, understands the claimed technical scheme of the present invention so that clear.
The extracting method of embodiment 1 desert Folium rosae multiflorae cell extract
An extracting method for desert Folium rosae multiflorae cell extract, concrete steps are as follows:
(1) select fresh, the complete and pure desert Flos Rosae Multiflorae leaf of high-quality, put into reducing mechanism after cleaning up and be ground into particle diameter≤40 object material powder;
(2) material powder is transported in supercritical extraction reactor, input CO in supercritical extraction reactor
2fluid, CO
2flow velocity is 20 ~ 25kg/h, extracting pressure is adjusted to 20 ~ 25MPa, extraction temperature is extract at 35 DEG C, extract 2 hours;
(3) take out extraction mixture from supercritical extraction reactor outlet, mixture is input in supercritical separating still, separating pressure is adjusted to 8 ~ 12MPa, temperature is 40 DEG C, be separated 1 hour;
(4) be that 1:50g/L is added to the water above separated product by solid-liquid ratio, straight fire adds thermal distillation 2 hours, and steam condensing reclaims and obtains desert Folium rosae multiflorae cell extract.
The application of embodiment 2 desert Folium rosae multiflorae cell extract in skin care item
Desert Folium rosae multiflorae cell extract is added in the Cosmetic Manufacture techniques such as protective skin cream.This prescription for skin cream contains following component: stearic acid 5%, isopropyl palmitate 4%, Span-602%, lanoline 3%, hexadecanol 2%, methyl hydroxybenzoate 0.2%, propylparaben 0.1%, monoglyceride 2%, white mineral oil 9%, essence 0.2%, desert Folium rosae multiflorae cell extract 4%, glycerol 4%, Tween-601.5%, DP-3000.1%, potassium hydroxide 0.2%, allantoin 0.3%, water 62.4%.
The irritation test of embodiment 3 desert Folium rosae multiflorae cell extract
Desert Folium rosae multiflorae cell extract is added in the production process of the cosmetics such as protective skin cream, and acute skin irritation test is carried out to white rabbit.
1. select the white rabbit that three Individual Sizes are similar and healthy, the hair of their spinal column both sides, back is cut 2 cm x 2 centimetres.
2. getting 0.3 gram of protective skin cream that with the addition of desert Folium rosae multiflorae cell extract directly spreads upon on the skin on the right side of white rabbit, covers on it with clean gauze, then with non-irritating immobilization with adhesive tape 2 hours.Side is as negative control in addition.
3., after removing protective skin cream, observe white rabbit contact site dermoreaction and carry out respective record, wherein the observation period is 1 hour, 6 hours, 12 hours, 48 hours, and result as shown in Table 1.
Table one: desert Folium rosae multiflorae cell extract protective skin cream carries out acute skin irritation result of the test to white rabbit
Can reach a conclusion thus: under this experiment condition, desert Folium rosae multiflorae cell extract protective skin cream is to white rabbit acute skin nonirritant.And through observing, find that there is skin that desert Folium rosae multiflorae cell extract protective skin cream smeared and more to add water profit, skin more compacts.Therefore desert Folium rosae multiflorae cell extract protective skin cream has and better has skin-moisturizing, skin, speckle dispelling, anti-ageing beauty functions of waiting for a long time.
The announcement of book and instruction according to the above description, those skilled in the art in the invention can also change above-mentioned embodiment and revise.Therefore, the present invention is not limited to detailed description of the invention disclosed and described above, also should fall in the protection domain of claim of the present invention modifications and changes more of the present invention.In addition, although employ some specific terms in this description, these terms just for convenience of description, do not form any restriction to the present invention.
Claims (9)
1. an extracting method for desert Folium rosae multiflorae cell extract, is characterized in that: the leaf getting fresh desert Flos Rosae Multiflorae, pulverizes; Material powder is sent in supercritical extraction reactor and carries out supercritical CO
2extraction; Get in extraction mixture feeding supercritical separating still and be separated; Separated product adds in distilled water and distills, and steam condensing reclaims and obtains desert Folium rosae multiflorae cell extract.
2. the extracting method of desert as claimed in claim 1 Folium rosae multiflorae cell extract, is characterized in that: physical pulverization desert Flos Rosae Multiflorae leaf is to particle diameter≤40 object material powder.
3. the extracting method of desert as claimed in claim 1 Folium rosae multiflorae cell extract, is characterized in that: supercritical CO
2during extraction, CO
2flow velocity is 20 ~ 25kg/h, and extracting pressure is 20 ~ 25MPa, extraction temperature is extract at 35 DEG C, extraction time 1 ~ 3 hour.
4. the extracting method of desert as claimed in claim 1 Folium rosae multiflorae cell extract, is characterized in that: separating pressure when supercritical is separated is 8 ~ 12MPa, temperature is 40 DEG C, is separated 1 hour.
5. the extracting method of desert as claimed in claim 1 Folium rosae multiflorae cell extract, is characterized in that: the product that supercritical is separated is 1:(40 ~ 60 in solid-liquid ratio) ratio of g/L is added to the water and distills.
6. the extracting method of desert as claimed in claim 1 Folium rosae multiflorae cell extract, is characterized in that concrete steps are as follows:
(1) select fresh, the complete and pure desert Flos Rosae Multiflorae leaf of high-quality, put into reducing mechanism after cleaning up and be ground into particle diameter≤40 object material powder;
(2) material powder is transported in supercritical extraction reactor, input CO in supercritical extraction reactor
2fluid, CO
2flow velocity is 20 ~ 25kg/h, extracting pressure is adjusted to 20 ~ 25MPa, extraction temperature is extract at 35 DEG C, extract 2 hours;
(3) take out extraction mixture from supercritical extraction reactor outlet, mixture is input in supercritical separating still, separating pressure is adjusted to 8 ~ 12MPa, temperature is 40 DEG C, be separated 1 hour;
(4) be that 1:50g/L is added to the water above separated product by solid-liquid ratio, straight fire adds thermal distillation 2 hours, and steam condensing reclaims and obtains desert Folium rosae multiflorae cell extract.
7. the application of desert Folium rosae multiflorae cell extract in skin care item described in claim 1 to 6 any one.
8. apply as claimed in claim 7, it is characterized in that: described skin care item are protective skin cream, facial film, facial cream, facial milk cleanser, cosmetic water, emulsion, essence, eye cream.
9. apply as claimed in claim 7, it is characterized in that: described skin care item are spot-eliminating skin care product, Skin whitening care cosmetics, Moisturizer.
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| CN201510074759.9A CN104622759A (en) | 2015-02-11 | 2015-02-11 | Extracting method and application for adenium obesum leaf cell extract |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105878131A (en) * | 2016-06-08 | 2016-08-24 | 广州市创美时美容化妆品有限公司 | Anti-aging cosmetic composition with plant-cell active substances and polypeptide |
| CN107233262A (en) * | 2017-07-27 | 2017-10-10 | 广州赛莱拉干细胞科技股份有限公司 | A kind of moisture-keeping composition and preparation method thereof |
| CN108498427A (en) * | 2018-06-11 | 2018-09-07 | 广东芭薇生物科技股份有限公司 | A kind of high osmosis moisturizing emulsion and preparation method thereof |
| CN109568210A (en) * | 2019-01-10 | 2019-04-05 | 广州睿森生物科技有限公司 | A kind of moisturizing remediation composition and its application |
| CN110013463A (en) * | 2019-04-29 | 2019-07-16 | 深圳津梁生活科技有限公司 | Whitening and softening skin freeze-drying facial mask containing desert rose essence and preparation method thereof |
| CN110585091A (en) * | 2019-10-25 | 2019-12-20 | 广州市拉凯尔干细胞研究所 | Preparation method of skin care product raw material containing desert rose leaf cell extract |
| CN110613650A (en) * | 2019-10-25 | 2019-12-27 | 上海伽誉生物科技有限公司 | Essence containing okra hydrolyzed glycoprotein SILKY-WSP and preparation method thereof |
| CN112322390A (en) * | 2020-10-20 | 2021-02-05 | 安徽省泰林生态农业科技发展有限公司 | Method for extracting essential oil of sequoia zhongshanensis |
| CN115589946A (en) * | 2022-10-18 | 2023-01-13 | 安赛搏（重庆）生物技术有限公司（Cn） | Preparation method and application of desert rose leaf callus extract |
| CN117180163A (en) * | 2023-11-08 | 2023-12-08 | 北京尧景基因技术有限公司 | Extraction method of desert rose callus vesicles and application of desert rose callus vesicles in preparation of skin whitening products |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105878131A (en) * | 2016-06-08 | 2016-08-24 | 广州市创美时美容化妆品有限公司 | Anti-aging cosmetic composition with plant-cell active substances and polypeptide |
| CN105878131B (en) * | 2016-06-08 | 2019-06-11 | 佛山市奥姿美生物科技有限公司 | A kind of anti-aging cosmetics composition containing plant cell active matter and polypeptide |
| CN107233262A (en) * | 2017-07-27 | 2017-10-10 | 广州赛莱拉干细胞科技股份有限公司 | A kind of moisture-keeping composition and preparation method thereof |
| CN108498427B (en) * | 2018-06-11 | 2021-03-16 | 广东芭薇生物科技股份有限公司 | High-permeability moisturizing emulsion and preparation method thereof |
| CN108498427A (en) * | 2018-06-11 | 2018-09-07 | 广东芭薇生物科技股份有限公司 | A kind of high osmosis moisturizing emulsion and preparation method thereof |
| CN109568210A (en) * | 2019-01-10 | 2019-04-05 | 广州睿森生物科技有限公司 | A kind of moisturizing remediation composition and its application |
| CN110013463A (en) * | 2019-04-29 | 2019-07-16 | 深圳津梁生活科技有限公司 | Whitening and softening skin freeze-drying facial mask containing desert rose essence and preparation method thereof |
| CN110585091A (en) * | 2019-10-25 | 2019-12-20 | 广州市拉凯尔干细胞研究所 | Preparation method of skin care product raw material containing desert rose leaf cell extract |
| CN110613650A (en) * | 2019-10-25 | 2019-12-27 | 上海伽誉生物科技有限公司 | Essence containing okra hydrolyzed glycoprotein SILKY-WSP and preparation method thereof |
| CN112322390A (en) * | 2020-10-20 | 2021-02-05 | 安徽省泰林生态农业科技发展有限公司 | Method for extracting essential oil of sequoia zhongshanensis |
| CN115589946A (en) * | 2022-10-18 | 2023-01-13 | 安赛搏（重庆）生物技术有限公司（Cn） | Preparation method and application of desert rose leaf callus extract |
| CN117180163A (en) * | 2023-11-08 | 2023-12-08 | 北京尧景基因技术有限公司 | Extraction method of desert rose callus vesicles and application of desert rose callus vesicles in preparation of skin whitening products |
| CN117180163B (en) * | 2023-11-08 | 2024-01-30 | 北京尧景基因技术有限公司 | Extraction method of desert rose callus vesicles and application of desert rose callus vesicles in preparation of skin whitening products |
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