CN104620832B - Ancient Cacumen Platycladi cuttage breeding method - Google Patents
Ancient Cacumen Platycladi cuttage breeding method Download PDFInfo
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Abstract
The invention discloses ancient Cacumen Platycladi cuttage breeding method.The invention provides a kind of Gu Cacumen Platycladi cuttage breeding method, comprise the steps：Cutting from ancient Cacumen Platycladi is used successively potassium permanganate, sodium lignate and HORMONE TREATMENT, the cutting after being processed；By the cutting cuttage in substrate after described process, cultivate, realize ancient Cacumen Platycladi cutting propagation.The experiment proves that, the method for the present invention, improves the survival rate of Cacumen Platycladi ancient tree clonal propagation, shortens Cacumen Platycladi ancient tree rootage duration, the research of other ancient tree clonal propagations is offered reference simultaneously.
Description
Technical field
The present invention relates to biological technical field, more particularly, to ancient Cacumen Platycladi cuttage breeding method.
Background technology
Cacumen Platycladi is long in China's cultivation history, and the life-span is up to the ancient Cacumen Platycladi of centuries, even thousand of years on the much ground in the whole nation
Qu Jun is distributed, and increases, with the ancient tree age, the stress that its inside and outside is subject to and is gradually increased, Cacumen Platycladi in thousand especially 3000 with
On Cacumen Platycladi entered high age, how effectively tree vigo(u)r is very weak, clonal propagation advanced age Cacumen Platycladi, be allowed to life forever continue,
Value shows, becomes global Chinese's question of common concern, also becomes the problem of local forest department urgent need to resolve.The age of tree is got over
Greatly, take root more difficulty from the point of view of domestic and international progress, at present some researchs existing, example in terms of the cutting propagation of centuries ancient tree
If 600 years Semen Ginkgo cutting plantation are 8%-25%.The age of tree is 300 years, the Ramulus et folium taxi cuspidatae of 500 years and 600 years is material
Carry out discovery during cuttage, with the cutting elite stand age of tree, the increase at branch age, rooting percent assumes reduction trend.In addition right
1300 about Semen Ginkgo and the cutting propagation of Sophora japonica L. also have been reported that, but ancient Sophora japonica L. cuttage does not survive.According to the U.S.《During New York
Report》Report, fox-brush pine (Bristlecone pine) from 4600 years old is cut after fringe is planted and is not but survived, the side more than 1000
The successful cutting propagation of Bai Gushu is still blank.
The age of tree is bigger, more difficult to take root, and the endogenous cause of ill of impact cutting propagation has：Age effect, position effect, colony and individuality are again
Difference of raw ability etc., the exopathogenic factor of impact cuttage expanding propagation mainly has growth regulator species and its concentration, substrate, illumination, warm and humid
Degree etc..
Content of the invention
It is an object of the present invention to provide a kind of Gu Cacumen Platycladi cuttage breeding method.
The method that the present invention provides, comprises the steps A：
Cutting from ancient Cacumen Platycladi is used potassium permanganate, sodium lignate and HORMONE TREATMENT successively, after being processed
Cutting；Cultivate the cutting cuttage in substrate after described process to taking root, realize ancient Cacumen Platycladi cutting propagation.
Ancient Cacumen Platycladi is more than the Cacumen Platycladi of 100 years for the age of tree.
In said method, described the cutting from ancient Cacumen Platycladi is used successively potassium permanganate, at sodium lignate and hormone
The method of reason comprises the steps：
1) cutting from ancient Cacumen Platycladi is soaked in potassium permanganate solution, obtain cutting after potassium permanganate is processed；
2) cutting after the process of described potassium permanganate is soaked in sodium lignate aqueous solution, obtain sodium lignate and process
Cutting afterwards；
3) cutting after the process of described sodium lignate is soaked in hormone solution, the cutting after being processed.
In said method, described hormone is NAA and IBA；
Described hormone solution is made up of solute and solvent, and described solute is NAA and IBA.
Described hormone solution preparation method：After hormone is dissolved with a small amount of ethanol, then it is mixed to get hormone solution with water.
In said method, the potassium permanganate weight/mass percentage composition in described potassium permanganate solution is 0.05%；
Lignin acid concentration in described sodium lignate aqueous solution is 500-2000mg.L-1；
In described hormone solution, NAA and IBA concentration is 600-4000mg.L-1.
In said method, described Gu Cacumen Platycladi and its corresponding sodium lignate and following 1) -4 of hormone concentration) in any one：
1) age of tree of described Gu Cacumen Platycladi is 100 years or 300 years or 3000, from the described cutting from ancient Cacumen Platycladi
Lignin acid concentration in corresponding described sodium lignate aqueous solution is 2000mg.L-1；From described from ancient Cacumen Platycladi
The corresponding described hormone solution of cutting in NAA and IBA concentration be 1000mg.L-1；
2) age of tree of described Gu Cacumen Platycladi is 700, from the corresponding described lignin of the described cutting from ancient Cacumen Platycladi
Lignin acid concentration in acid sodium aqueous solution is 500mg.L-1；Corresponding described sharp from the described cutting from ancient Cacumen Platycladi
In plain solution, NAA and IBA concentration is 600mg.L-1.
In said method, step 2) in, from the age of tree be 100 years or 300 years or 3000 ancient Cacumen Platycladis cutting in wood
Quality acid concentration is 2000mg.L-1Sodium lignate aqueous solution in soak 1min,
From the age of tree be 700 years ancient Cacumen Platycladis cutting in 500mg.L-1Sodium lignate aqueous solution in soak 2h,
Step 3) in, the cutting being 100 years or 300 years or 3000 ancient Cacumen Platycladis from the age of tree is equal in NAA and IBA concentration
For 1000mg.L-1Hormone solution in soak 1min；
From the age of tree be 700 years ancient Cacumen Platycladis cutting in 600mg.L-1Solute be NAA and IBA hormone solution in soak
Bubble 1h.
In said method, the described cutting from ancient Cacumen Platycladi is described Gu Cacumen Platycladi tree crown sunny side top (tree crown top two
3/1 mono- to four positions) 1-3 life branch.
In said method, described cultivate in described cutting is carried out disinfection, described sterilization adopt biocide be methyl-
1H-2- benzimidazole carbamate, thiophanate-methyl, Mancozeb, Bordeaux mixture, thiophanate-methyl MnZn and ziram
At least two in this 6 kinds.
Above-mentioned disinfection way is that 6 kinds of rotations use.
It is a further object to provide a kind of method cultivating ancient Cacumen Platycladi cutting seedling.
The method that the present invention provides, including described step A in above-mentioned cottage method
The age of tree of ancient Cacumen Platycladi is 100 years or 300 years or 700 years or 3000.
The experiment proves that, this research makes full use of the Cacumen Platycladi of all ages and classes, carries out cutting propagation phase by comprehensive
Close technical research, explored through nearly 2 years, tentatively captured a Technique on Cuttage Propagation difficult problem, groped the cuttage of a set of Cacumen Platycladi ancient tree
Breeding system, and obtain Cacumen Platycladi cuttage in 3000 11 plants of Seedling of clone.This research is not only protection and clonal propagation Cacumen Platycladi ancient tree
Lay a good foundation, provide direction for other ancient tree asexual propagation.
Ancient tree clonal propagation systems are to the preservation of ancient tree excellent genes, cultivation technique results transferring, technical advice and training etc.
Aspect will create very big economic benefit.The ancient tree excellent genes remaining be used for the plant such as jujube tree, Camellia sinensis breeding and
Genetic improvement and popularization can bring huge economic benefit to self-employed tree cultivator's industry again.Ancient tree suffers from the background of deep culture, such as yellow
The Xuanyuan cypress on Supreme Being mound is referred to as " father of world Cupressaceae " by Englishman, and ancient tree clonal propagation can preserve the complete genome of ancient tree, it is to avoid
Forest Culture and tourist culture legacy disappear, significant.
Brief description
Fig. 1 is different parts rooting percent.
Fig. 2 is cutting Callus induction rate after mortifier removing.
Fig. 3 is rooting percent after mortifier removing.
Specific embodiment
Experimental technique used in following embodiments if no special instructions, is conventional method.
Material used, reagent etc. in following embodiments, if no special instructions, all commercially obtain.
In following embodiments, the method for cuttage is as follows：
(1) cutting is taken
2013-2014 clip with 5 years raw Cacumen Platycladis be comparison, take the Temple of Heaven 100 years, 300 years, the Cacumen Platycladi of 700 years
On the 3 years raw branches in raw Cacumen Platycladi (Huang Di Mausoleum Xuanyuan, Shaanxi cypress) tree crown south middle and lower part in about 3000 first round mitogenetic, selection
Robust growth, the branch (for cutting) of no disease and pests harm, encase otch, spray water with l Water Paper, bubble chamber is taken back.
(2) cutting preparation and process
By the branch taken back, each is cut into 20 about cutting (each 5～10cm), lower cut from bud 1～1.5cm, tiltedly
Cut, otch should smooth, with blade rip cutting 2～3 knife at cutting base portion 1～1.5cm, as deep as xylem, every 20 one after shearing
Bundle.The carbendazim solution that cutting is immediately placed in 1 ‰ soaks 15min to sterilize, and is then cleaned with clear water.Soaked from hormone.Cuttage
Depth 8～10cm.It is compacted substrate after inserting, and sprayed water 1 time with watering can immediately.
(3) substrate prepares
Peat：Perlite=3：7
(4) slotting machine prepares
In Chinese forest-science academy, be provided with the circular slotting machine that radius is 6m, slotting machine is provided with intermittent spraying device, front thin after
Close, often hold and put 25, shower nozzle.Before cuttage, 1d is carried out disinfection to cutting medium with 3 ‰ potassium permanganate, and cuttage same day clear water pours
Thoroughly.
Embodiment 1, the cuttage fringe part of ancient Cacumen Platycladi are groped
First, hormon processes different age of tree Gu Cacumen Platycladi rooting percents
Auxin has the obvious effect promoting adventitious root and lateral root generation and growth, because different growth hormone are to slotting
The suitability that fringe is taken root is inconsiderate, and different plant is also different to the adaptability of different growth hormone, therefore, selects suitable species
Growth hormone is particularly important to Cacumen Platycladi ancient tree cuttage.Hormone select cuttage experiment in more common species ABT1, NAA and
NAA:IBA=1:1 this 3 kinds of hormones, concentration design is divided into high concentration, middle concentration and low concentration.
ABT1 solution, NAA solution and NI solution (NAA:IBA=1:1) prepare hormon solution according to different concentration,
Concentration design is divided into low concentration (200-600mg.L-1；Shown in table 1), middle concentration (1000-3000mg.L-1；Shown in table 2) and
High concentration (4000-10000mg.L-1；Shown in table 3).
With 5 years raw Cacumen Platycladis for comparison, take the Temple of Heaven 100 years, 300 years, the Cacumen Platycladi of 700 years tested as follows：
Soak 1- by after the Gu Cacumen Platycladi cutting sterilization of the different age of trees in the aqueous solution containing hormon (shown in table 1-3)
2h, then cuttage in substrate, cultivate.
After cultivating 45d, the amount/cuttage cutting of wound healing rate=generation calluss cutting of detection different age of tree Gu Cacumen Platycladi
Total amount；
After cultivating 90d, the total amount of the amount/cuttage cutting of rooting rate=cutting of taking root of detection different age of tree Gu Cacumen Platycladi.
Result is as follows：
1st, the impact to Cacumen Platycladi ancient tree cuttage root-taking for the low concentration hormone
When using low concentration HORMONE TREATMENT cutting shown in table 1, result such as 1,5 years raw treelet 600mg.L of table-1ABT1
Solution processes 1h and 400mg.L-1NI solution (NAA:IBA=1:1) process 1h and all can reach preferable effect, its wound healing induces
Rate and rooting rate are 100%, and raw Cacumen Platycladi is with 200mg.L within 100 years-1ABT1 solution processes 2h can have the maximum Callus induction rate to be
18%, final rooting rate is 12%.And 300 years and 700 years Cacumen Platycladi cutting are with 200mg.L-1NI solution (NAA:IBA=1:1) place
Reason 4h, Callus induction rate is 23% and 32% respectively, and rooting rate is 14% and 15%.
Table 1. low concentration hormone solution processes all ages and classes Cacumen Platycladi Callus induction rate, rooting rate
Note：A represents ABT1, and N represents NAA, and NI represents NAA:IBA=1:1, A200 represents that hormone concentration is 200mg.L-1's
ABT1, similarly hereinafter, NI200 represents that concentration in NI solution for NAA and IBA is 200mg.L-1.
2nd, the impact to Cacumen Platycladi ancient tree cuttage root-taking for the middle concentration hormone
When using middle concentration HORMONE TREATMENT cutting shown in table 2, result such as 2,5 years raw treelet Callus induction rate of table all exist
More than 50%, wherein use 1000mg.L-1ABT1 solution processes 1min and 1000mg.L-1NI solution processes 30s and all can reach relatively
Good rooting efficiency, its rooting rate is 88%, and the latter has higher Callus induction rate after treatment, is 100%.Life in 100 years
Cacumen Platycladi is with 2000mg.L-1NI solution processes 3min and 3000mg.L-1NI solution processes 1min Callus induction rate and all reaches 33%, and
Rooting rate difference less, is 19% and 18% respectively.Cacumen Platycladi cutting is with 1000mg.L within 300 years-1After NI solution processes 30s, wound healing
Inductivity is 48%, and rooting rate is up to 24%.And Cacumen Platycladi cutting was with 1000mg.L in 700 years-1ABT1 solution processes 1min effect
Good, there is 20% cutting can take root, 40% cutting can break up calluss.
Concentration HORMONE TREATMENT all ages and classes Cacumen Platycladi Callus induction rate, rooting rate in table 2
Note：A represents ABT1, and N represents NAA, and NI represents NAA:IBA=1:1, A200 represents that hormone concentration is 200mg.L-1's
ABT1, similarly hereinafter.
3rd, the impact to Cacumen Platycladi ancient tree cuttage root-taking for the High concentration auxin
High concentration auxin using table 3 processes cutting, result as shown in table 3,5 years raw treelets and 100 years Cacumen Platycladi cutting
Use 8000mg.L-1Callus induction rate highest when ABT1 solution processes 5s, respectively 99% and 67%, finally take root, have substantially
Difference, raw treelet has 83% to take root within 5 years, and then only has within 100 years 18% and take root.300 years and 700 years Cacumen Platycladi cutting then with
4000mg.L-1When NI solution processes 5s, effect is preferable.
Table 3 High concentration auxin processes all ages and classes Cacumen Platycladi Callus induction rate, rooting rate
Note：A represents ABT1, and N represents NAA, and NI represents NAA:IBA=1:1, A200 represents that hormone concentration is 200mg.L-1's
ABT1, similarly hereinafter.
Just all in all, using High concentration auxin process cutting when, its rooting efficiency do not use in concentration HORMONE TREATMENT
Cutting good.
After hormones recipe determination, according to the experimental result tentatively drawing, be conducive to improving advanced age side
The optimal root-inducing promoter of cypress (age of trees of especially 300 years and 700 years) cuttage rooting ability is NAA:IBA=1:1, further
This combination formula is refined, by setting variable concentrations and process time, is matched out optimal scheme further.
2nd, the impact to cuttage rooting for the NI different disposal
With 5 years raw Cacumen Platycladis for comparison, take the Temple of Heaven 100 years, 300 years, the Cacumen Platycladi of 700 years tested as follows：
In variable concentrations NI solution (NAA after different age of trees Gu Cacumen Platycladi cutting is sterilized:IBA=1:1；Table 4) middle immersion 4h-
1min time, then cuttage in substrate, cultivate.
Wound healing rate and rooting rate, the result such as table 4 of different age of tree Gu Cacumen Platycladis is detected, all ages and classes cutting institute is right after four months
The optimal HORMONE TREATMENT concentration answered is different.Wherein 5 years raw treelets and 100 years Cacumen Platycladi cutting are with 1000mg.L-1NI solution is processed
2min Callus induction rate is respectively 90% and 67%, and rooting rate is 79% and 25%.300 years Cacumen Platycladi cutting be then with
1000mg.L-1NI solution processes 1min and reaches higher rooting rate, is 20%, and the Callus induction rate of 700 years Cacumen Platycladi cutting reaches
53%, the hormone being used is 600mg.L-1NI solution processes 1h, and highest rooting rate reaches 16%.
Table 4 variable concentrations NI solution processes all ages and classes rooting percent
As can be seen that 1000mg.L-1(NAA and IBA is 1000mg.L to NI solution-1), be 100 years, 300 Cacumen Platycladis optimal
NI concentration, for 700 years, NAA:IBA concentration is 600mg.L-1, for optimal NI concentration.
Consider process time, then select NAA:IBA is 1000mg.L-1Optimum N I concentration as ancient Cacumen Platycladi carries out following reality
Test and grope.
3rd, adopt the impact to cuttage root-taking for the bar position
Inserted part on elite stand for the cutting is different, and cell division capacity is different, take root promotion material and rooting inhibition of cuttings
Also difference, the impact generation of calluss and the formation of adventitious root of the content of material, leads to rootability different.One
As think, the cutting taken from tree crown bottom, the obstruction material by being formed is few, and nutrition content is higher, so taking root
Rate is high.But also it is believed that being in optical position on tree crown, branch flushes, due to being covered, branch is often given birth to for bottom
Length is bad, and therefore branch rootability in bottom is not so good as top.
Take that Beijing the Temple of Heaven 100 years Cacumen Platycladis east is upper, under east, Nan Shang, go down south, west is upper, western under, go up north, northern lower 8 sides
The cutting of position, and select concentration 1000mg.L after sterilizing-1(NAA and IBA is 1000mg.L to NI solution-1) process 1min, then
Cuttage in substrate, cultivates (table 5).
After cuttage four months, the cuttage experiment that difference is adopted with bar position carries out wound healing rate and rooting rate statistics.Result is as schemed
1, being easier of the cutting lower part cutting positioned at tree crown top is taken root, and is located at elite stand cutting in the south compared with other orientation cutting
Callus induction rate high, up to 47%, and rooting rate is up to 42%.Therefore, when choosing cutting, (to lean on positioned at top in the south
Upper 1/2 to 3/4 tree crown) cutting is optimal.
Table 5 is to adopt the impact to cuttage root-taking for the bar position
4th, endogenous Rooting is removed
Cutting is more difficult to take root, may be relevant with there are some obstruction materials of taking root in vivo.In general, the content of inhibitor
Can increase with the increase of parental age, accordingly raise with the increase of shoot maturation degree.On same tree crown, bottom presses down
The content of preparation can be less than top inhibitor content.Inhibitor hinders the supposition taken root of cutting to have two kinds：One is to weaken or hinder
The only effect of auxin；Two is by being trapped in cutting cut surface, such as resin or tannin, thus affecting the suction of cutting base portion
Water.
Substantial amounts of experiment shows, the endogenous Rooting of impact plant cuttage root-taking is mainly phenols, flavonoid, tannin
And resin etc., therefore mortifier removes experiment, carries out mainly for this several material, and combines Measure choice used in document
Reset mode.
HORMONE TREATMENT selects cuttage root-taking effect preferable concentration 1000mg.L-1NI solution, and (NAA and IBA is
1000mg.L-1) cutting is processed, the different impact to Cacumen Platycladi cuttage for the mortifier reset mode of research, experimental design such as table
6, specific as follows：
With 5 years raw Cacumen Platycladis for comparison, take the Temple of Heaven 100 years, 300 years, the top in the south of the Cacumen Platycladi tree crown of 700 years
Cutting, after sterilization, be separately immersed in 0.1% silver nitrate aqueous solution, 0.1% aqueous acetic acid, 2% ethanol water, water, wash clean
Essence, 0.05% potassium permanganate solution different time, then (NAA, IBA and water mix, and obtain with concentration 1000mg.L-1NI solution
Aqueous solution, makes the final concentration of NAA and IBA be 1000mg.L-1) process 1min, then cuttage in substrate, cultivate.
After cuttage four months, cuttage rooting situation is investigated, its result such as Fig. 2 and Fig. 3, Fig. 2 are Callus induction rate
As a result, Fig. 3 removes result for mortifier.As can be seen that process 5 years raw treelets with 0.1% silver nitrate inserting
Fringe, can make its Callus induction rate reach 90%, rooting rate reaches 62%.And 100 years, 300 years and 700 years Cacumen Platycladis are inserted
For fringe, use 0.05%KMnO4Process then can reach preferable rooting efficiency, Callus induction rate be respectively 37%, 43% and
36%, rooting rate is respectively 20%, 17% and 12%.
Result shows, all ages and classes, different mortifier reset modes have larger difference to the impact of Cacumen Platycladi cuttage rooting.Always
For body, with KMnO4Process most useful for more than the Cacumen Platycladi cutting calli induction of 100 years age of trees and cuttage rooting, and
0.1% acetic acid processes and is removed without obvious effect to cutting mortifier.
Table 6 mortifier removes cuttage experiment
5th, the impact to all ages and classes Cacumen Platycladi cuttage root-taking for the plant growth regulator
Certain nutrient substance, mainly carbohydrate and nitrogen compound is needed during cuttage rooting.Water-soluble with sucrose
Liquid processes cutting, and hestening rooting effect is more preferable.Additionally, sodium lignate (ASL), as plant growth regulators, has rush
Enter plant wound heeling, cutting root of hair, improve percentage of seedgermination and accelerate the effect such as seedling growth, and forest seedling can be improved
Resistance.Presoak cutting with certain density ASL, in certain concentration range, have obvious facilitation to taking root.Cause
This, contrived experiment probes into the optimal ASL concentration of suitable Cacumen Platycladi cutting propagation, and with the feelings of taking root after aqueous sucrose solution process
Condition, specific as follows：
With 5 years raw Cacumen Platycladis for comparison, take the Temple of Heaven 100 years, 300 years, the inserting of Cacumen Platycladi tree crown the south top of 700 years
Fringe, after sterilization, is immersed in 0.05% potassium permanganate solution 15min time, then the sodium lignate using variable concentrations respectively
(ASL) aqueous solution and 10% aqueous sucrose solution soak different time, then use concentration 1000mg.L-1NI solution processes 1min, then
Cuttage in substrate, cultivates.
After four months, cuttage rooting situation is counted, its result such as table 7.
Table 7 variable concentrations ASL processes Cacumen Platycladi Callus induction rate, rooting rate
As can be seen from Table 7, with certain density ASL and saccharose treatment Cacumen Platycladi cutting, its rooting efficiency is had one be set for
With.All ages and classes desired concn is different.Raw treelet is with 200mg.L within 5 years-1Process 4h and can form more calluss, rooting rate is high
Reach 93%.100 years Cacumen Platycladi 1000mg.L-1ASL aqueous solution processes 3min and 2000mg.L-1ASL aqueous solution processes 1min and obtains
It is 45% to identical rooting rate, the Callus induction rate of the latter is higher.Variable concentrations ASL processes 300 years cutting, shadow that it is taken root
Ring less.And for 700 years Cacumen Platycladi cutting, the long-time immersion of low concentration is more conducive to it and takes root.The shadow to cuttage rooting for the sucrose
Sound also has certain age effect, but generally facilitates taking root of cutting.
Consider matter of time, 100 years and 300 years Cacumen Platycladis select 2000mg.L-1ASL aqueous solution soaking 1min, 700 years Cacumen Platycladis
From 500mg.L-1ASL aqueous solution soaking 2h.
6th, biocide screening
After cuttage, cutting is vulnerable to virus infection and direct sunlight excessively leads to temperature too high and burn seedlings dehydration death, because
Sterilization rooting treatment is periodically done after this cuttage.Biocide includes：Carbendazim, thiophanate methyl (thiophanate-methyl), Mancozeb,
Bordeaux mixture, thiophanate-methyl MnZn, good fortune good fortune zinc etc., specific as follows：
Take the cutting on the Temple of Heaven Cacumen Platycladi tree crown of 100 years the south top, after sterilization, be immersed in 0.05% potassium permanganate
The aqueous solution 15min time, then use 2000mg.L-1ASL aqueous solution soaking 1min, then processed with concentration 1000mg.L-1NI solution
1min, then cuttage in substrate, cultivate.Nurturing period, on every Mondays, four pairs of nursery stocks and seedbed carry out pasteurised completely sterilizing, directly
To cuttage root-taking (rootage duration is to start to growing first adventitious root time only from cuttage, and rootage duration is 3 months), disappear
Malicious mode is following 1) or 2)：
1) carbendazim：
Zhejiang spring scenery biochemistry company limited；Product registration number：LS95505
Carbendazim (methyl isophthalic acid H-2- benzimidazole carbamate) dilutes 500 times, and 2g converts 1kg water, on every Mondays, four respectively sprays
Spill once, every square metre of sprinkling about 1kg solution.
2) 6 kinds of biocide wheels are used
Carbendazim (methyl isophthalic acid H-2- benzimidazole carbamate), thiophanate methyl (thiophanate-methyl), Mancozeb,
Bordeaux mixture, first frost MnZn and this 6 kinds of biocide wheels of Fu Fu zinc (PZ) are used, on every Mondays,
Once, the biocide spraying every time is different, and the order adopting in the present embodiment is for four each sprinklings：Monday sprays thiophanate methyl
(thiophanate-methyl), Thursday spray Mancozeb, second Monday spray Bordeaux mixture, second Thursday spray Bordeaux mixture, the 3rd
Monday sprays thiophanate-methyl, the 3rd Thursday spray MnZn and ziram (PZ)
Thiophanate-methyl (thiophanate methyl SC)：Longdeng Chemical Co Ltd, Jiangsu；Product registration number：PD20080138
Thiophanate methyl dilutes 500 times, and 2g converts 1kg water, every square metre of sprinkling about 1kg solution.
Mancozeb：Limin Chemical Co., Ltd.；Product registration number：PD20040011
Mancozeb dilutes 500 times, and 2g converts 1kg water, every square metre of sprinkling about 1-2kg solution.
Bordeaux mixture：Ying Sui Guang Nongzhensheng Chemical Co., Ltd. of Guangdong Province；Product registration number：LS20003F030332
Bordeaux mixture dilutes 1000 times, and 1g converts 1kg water, every square metre of sprinkling about 1-2kg solution.
First frost MnZn：Zhejiang Prov HeBen pesticide Chemical Co., Ltd；Product registration number：PD20086169
First frost MnZn dilutes 500 times, and 2g converts 1kg water, every square metre of sprinkling about 1-2kg solution.
Good fortune good fortune zinc (PZ)：Hebei Zan Feng biological engineering company limited；
Good fortune good fortune zinc dilutes 250 times, and 4g converts 1kg water, every square metre of sprinkling about 1-2kg solution.
Result as shown in table 8, draws, multiple biocides are in turn using the effect obtaining after carrying out a large amount of biocide screenings
Most preferably.
Table 8 is greenery rate after raw Cacumen Platycladi sterilizing in 100 years
Embodiment Cacumen Platycladi Technique on Cuttage Propagation system in 2,3000
Each processes 20.It is repeated 3 times.
Cacumen Platycladi in 3000 is larger due to the age of tree, and cutting propagation is more difficult, using the choosing of all ages and classes Cacumen Platycladi cutting propagation
On the basis of allotting preferable hormone combination and cutting process, inquire into the Technique on Cuttage Propagation system of Xuanyuan cypress further, to
Make its reproductive success using cuttage mode.Preferably hormone ABT1 and NAA in the above results is selected during cuttage:IBA(1:1)
Cuttage is carried out to Xuanyuan cypress.During cuttage, cutting growing state is observed, with rooting rate quantity as index, specifically such as
Under：
Take within 2013 3000 of different months Cacumen Platycladi (the Shaanxi Xuanyuan cypress of Baidupedia or《In tree, the elderly person for whom a birthday celebration is being held knows
How much》、《The many wonders of China ancient tree》) tree crown the south top cutting, after sterilization, be immersed in 0.05% potassium permanganate solution
The 15min time, then use 2000mg.L-1ASL aqueous solution soaking 1min, then variable concentrations hormon solution is processed in table 9 respectively,
Cuttage in substrate again, cultivates.Nurturing period, on every Mondays, four pairs of nursery stocks and seedbed carry out pasteurised completely sterilizing, until cuttage
Take root, disinfection way is that several biocide wheels that in embodiment 1, table 8 adopts are used.Result such as table 9 and table 10.
9 3000 years Cacumen Platycladi rooting rates of table
10 3000 years Cacumen Platycladi cuttage system for handling of table
Experimentation can be seen, the system being conducive to Cacumen Platycladi cutting survival in 3000 is：June takes 3000
The raw then stalwartness coppice shoot NAA of the southern middle and upper part of Cacumen Platycladi (Huang Di Mausoleum Xuanyuan cypress):IBA(1:1)1000mg.L-1Cacumen Platycladi in 3000
The rooting rate processing 1min is up to 2.5%, in the meantime, by being periodically cutting sterilization, and carries out moisture, temperature
Control management.Under above-mentioned system condition, in November, 2013, existing 11 cutting are successfully taken root and well-grown (does altogether
1000 cutting), 6 plants of seedling of survival, average height 16.75cm at present, plant height is respectively：27cm、23cm、19.5cm、14cm、
11cm、6cm.
Claims (6)
1. a kind of Gu Cacumen Platycladi cuttage breeding method, comprises the steps A：
Cutting from ancient Cacumen Platycladi is used successively potassium permanganate, sodium lignate and HORMONE TREATMENT, the cutting after being processed；
Cultivate the cutting cuttage in substrate after described process to taking root, realize ancient Cacumen Platycladi cutting propagation；
Described the cutting from ancient Cacumen Platycladi is included with potassium permanganate, sodium lignate and hormone-treated method successively as follows
Step：
1) cutting from ancient Cacumen Platycladi is soaked in potassium permanganate solution, obtain cutting after potassium permanganate is processed；
2) cutting after the process of described potassium permanganate is soaked in sodium lignate aqueous solution, obtain inserting after sodium lignate is processed
Fringe；
3) cutting after the process of described sodium lignate is soaked in hormone solution, the cutting after being processed；
Described hormone solution is made up of solute and solvent, and described solute is NAA and IBA；
Potassium permanganate weight/mass percentage composition in described potassium permanganate solution is 0.05%；
Lignin acid concentration in described sodium lignate aqueous solution is 500-2000mg.L-1；
In described hormone solution, NAA and IBA concentration is 600-4000mg.L-1.
2. method according to claim 1 it is characterised in that：
Described Gu Cacumen Platycladi and its corresponding sodium lignate and following 1) of hormone concentration or 2)：
1) age of tree of described Gu Cacumen Platycladi is 100 years or 300 years or 3000, corresponding described from the cutting of described Gu Cacumen Platycladi
Lignin acid concentration in sodium lignate aqueous solution is 2000mg.L-1；Corresponding described from the cutting of described Gu Cacumen Platycladi
In hormone solution, NAA and IBA concentration is 1000mg.L-1；
2) age of tree of described Gu Cacumen Platycladi is 700 years, water-soluble from the corresponding described sodium lignate of cutting of described Gu Cacumen Platycladi
Lignin acid concentration in liquid is 500mg.L-1；From described Gu Cacumen Platycladi the corresponding described hormone solution of cutting in NAA and
IBA concentration is 600mg.L-1.
3. method according to claim 1 and 2 it is characterised in that：
Step 2) in, the cutting being 100 years or 300 years or 3000 ancient Cacumen Platycladis from the age of tree in lignin acid concentration is
2000mg.L-1Sodium lignate aqueous solution in soak 1min,
From the age of tree be 700 years ancient Cacumen Platycladis cutting in 500mg.L-1Sodium lignate aqueous solution in soak 2h,
Step 3) in, the cutting being 100 years or 300 years or 3000 ancient Cacumen Platycladis from the age of tree is in NAA and IBA concentration
1000mg.L-1Hormone solution in soak 1min；
From the age of tree be 700 years ancient Cacumen Platycladis cutting in 600mg.L-1Solute be NAA and IBA hormone solution in soak
1h.
4. method according to claim 1 and 2 it is characterised in that：The described cutting from ancient Cacumen Platycladi is described Gu side
Cupressaceae hat sunny side top 1-3 gives birth to branch.
5. method according to claim 1 and 2 it is characterised in that：In described cultivation, described cutting is carried out disinfection, described
Sterilization adopt biocide be methyl isophthalic acid H-2- benzimidazole carbamate, thiophanate-methyl, Mancozeb, Bordeaux mixture,
At least two in thiophanate-methyl MnZn and this 6 kinds of Fu Fu zinc.
6. a kind of method cultivating ancient Cacumen Platycladi cutting seedling, including described step A in any one of claim 1-5.
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| CN108093904A (en) * | 2017-12-18 | 2018-06-01 | 山东省林业科学研究院 | A kind of method using red beech perennial branch cutting propagation |
| CN108990572A (en) * | 2018-08-18 | 2018-12-14 | 西南林业大学 | A kind of Gu raising seedlings by cuttage for Poplars |
| CN109220270A (en) * | 2018-11-06 | 2019-01-18 | 北京林业大学 | A kind of Magnolia wufengensis softwood cutting propagation method based on optimal hormone combination |
| CN110800486B (en) * | 2019-10-21 | 2022-02-22 | 中国林业科学研究院林业研究所 | Method for improving ancient tree cuttage survival rate |
| CN112369229B (en) * | 2020-09-09 | 2022-10-04 | 北京市农林科学院 | Cuttage propagation method for platycladus orientalis through electric heating hotbed in winter |
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| CN115136801A (en) * | 2022-07-18 | 2022-10-04 | 中国林业科学研究院林业研究所 | Full-illumination spraying cuttage method for ancient arborvitae |
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