CN104614513A - 一种基于磁分离的弛豫时间免疫传感分析方法 - Google Patents
一种基于磁分离的弛豫时间免疫传感分析方法 Download PDFInfo
- Publication number
- CN104614513A CN104614513A CN201510038729.2A CN201510038729A CN104614513A CN 104614513 A CN104614513 A CN 104614513A CN 201510038729 A CN201510038729 A CN 201510038729A CN 104614513 A CN104614513 A CN 104614513A
- Authority
- CN
- China
- Prior art keywords
- magnetic
- magnetic bead
- antibody
- particle diameter
- magneto separate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510038729.2A CN104614513B (zh) | 2015-01-26 | 2015-01-26 | 一种基于磁分离的弛豫时间免疫传感分析方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510038729.2A CN104614513B (zh) | 2015-01-26 | 2015-01-26 | 一种基于磁分离的弛豫时间免疫传感分析方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104614513A true CN104614513A (zh) | 2015-05-13 |
CN104614513B CN104614513B (zh) | 2016-05-25 |
Family
ID=53149053
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510038729.2A Active CN104614513B (zh) | 2015-01-26 | 2015-01-26 | 一种基于磁分离的弛豫时间免疫传感分析方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104614513B (zh) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104871026A (zh) * | 2012-12-17 | 2015-08-26 | 通用电气公司 | 靶物质的体外磁共振检测 |
CN109550531A (zh) * | 2019-01-28 | 2019-04-02 | 武汉纺织大学 | 一种磁性尺寸依赖的微流控芯片 |
CN110187104A (zh) * | 2019-06-13 | 2019-08-30 | 华中农业大学 | 基于生物正交反应的横向弛豫时间免疫传感器的制备方法、传感器及其应用 |
CN110455848A (zh) * | 2018-05-08 | 2019-11-15 | 国家纳米科学中心 | 基于络合反应放大信号的铁离子纵向弛豫时间传感器及其构建方法、用途 |
CN110470688A (zh) * | 2019-07-29 | 2019-11-19 | 华中农业大学 | 一种纳米螯合筛介导的低场核磁共振免疫传感器及其应用 |
CN110499234A (zh) * | 2019-09-03 | 2019-11-26 | 浙江省人民医院 | 念珠菌检测设备及检测方法 |
CN110726841A (zh) * | 2019-10-21 | 2020-01-24 | 华中农业大学 | 一种基于酶促点击反应信号放大磁弛豫时间免疫传感器检测兽药残留的方法 |
CN111198267A (zh) * | 2020-01-09 | 2020-05-26 | 华中农业大学 | 一种磁弛豫时间免疫传感器磁信号探针及其应用 |
CN111208287A (zh) * | 2020-01-16 | 2020-05-29 | 长沙理工大学 | 一种磁共振传感器的构建方法 |
CN111351812A (zh) * | 2018-12-20 | 2020-06-30 | 中国农业大学 | 一种基于低场核磁共振成像的铜绿假单胞菌检测方法 |
CN112611868A (zh) * | 2020-12-18 | 2021-04-06 | 厦门大学 | 一种用于磁共振分析方法检测新型冠状病毒的探针及其制备方法 |
CN112964868A (zh) * | 2021-02-05 | 2021-06-15 | 华中农业大学 | 一种基于磁分离同时检测多种目标物的生化分析方法 |
CN114966012A (zh) * | 2022-04-07 | 2022-08-30 | 宁波大学 | 基于超顺磁性二维材料的低场核磁共振均相免疫检测食源性致病菌的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1176001A (zh) * | 1995-01-27 | 1998-03-11 | 舍林股份公司 | 磁力弛豫检测分析物的方法和化合物以及它们的用途 |
WO2012044387A2 (en) * | 2010-07-06 | 2012-04-05 | T2 Biosystems, Inc. | Methods and compositions for detection of analytes |
CN103278521A (zh) * | 2012-11-30 | 2013-09-04 | 中国检验检疫科学研究院 | 一种检测生物大分子的磁共振免疫传感方法 |
CN103364428A (zh) * | 2013-01-19 | 2013-10-23 | 南昌大学 | 一种基于γ-Fe2O3的NMR纳米探针食源性致病菌的快速检测方法 |
CN103865752A (zh) * | 2014-03-07 | 2014-06-18 | 复旦大学附属中山医院 | 循环肿瘤细胞捕获和分类磁性微流控芯片及其制造和使用 |
CN103954775A (zh) * | 2014-05-12 | 2014-07-30 | 国家纳米科学中心 | 一种检测生物大分子或微生物体的方法 |
-
2015
- 2015-01-26 CN CN201510038729.2A patent/CN104614513B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1176001A (zh) * | 1995-01-27 | 1998-03-11 | 舍林股份公司 | 磁力弛豫检测分析物的方法和化合物以及它们的用途 |
WO2012044387A2 (en) * | 2010-07-06 | 2012-04-05 | T2 Biosystems, Inc. | Methods and compositions for detection of analytes |
CN103278521A (zh) * | 2012-11-30 | 2013-09-04 | 中国检验检疫科学研究院 | 一种检测生物大分子的磁共振免疫传感方法 |
CN103364428A (zh) * | 2013-01-19 | 2013-10-23 | 南昌大学 | 一种基于γ-Fe2O3的NMR纳米探针食源性致病菌的快速检测方法 |
CN103865752A (zh) * | 2014-03-07 | 2014-06-18 | 复旦大学附属中山医院 | 循环肿瘤细胞捕获和分类磁性微流控芯片及其制造和使用 |
CN103954775A (zh) * | 2014-05-12 | 2014-07-30 | 国家纳米科学中心 | 一种检测生物大分子或微生物体的方法 |
Non-Patent Citations (1)
Title |
---|
YI PING CHEN: "An immunosensorbased on magnetic relaxation switch and polystyrenemicroparticle-induced immune multivalency enrichment system for the detection of Pantoea stewartii subsp.Stewartii", 《BIOSENSORS AND BIOELECTRONICS》 * |
Cited By (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10156567B2 (en) | 2012-12-17 | 2018-12-18 | General Electric Company | In-vitro magnetic resonance detection of a target substance without separating bound magnetic nanoparticles from unbound magnetic nanoparticles |
CN104871026A (zh) * | 2012-12-17 | 2015-08-26 | 通用电气公司 | 靶物质的体外磁共振检测 |
CN104871026B (zh) * | 2012-12-17 | 2020-01-10 | 通用电气公司 | 靶物质的体外磁共振检测 |
CN110455848A (zh) * | 2018-05-08 | 2019-11-15 | 国家纳米科学中心 | 基于络合反应放大信号的铁离子纵向弛豫时间传感器及其构建方法、用途 |
CN110455848B (zh) * | 2018-05-08 | 2024-02-23 | 国家纳米科学中心 | 基于络合反应放大信号的铁离子纵向弛豫时间传感器及其构建方法、用途 |
CN111351812A (zh) * | 2018-12-20 | 2020-06-30 | 中国农业大学 | 一种基于低场核磁共振成像的铜绿假单胞菌检测方法 |
CN109550531A (zh) * | 2019-01-28 | 2019-04-02 | 武汉纺织大学 | 一种磁性尺寸依赖的微流控芯片 |
CN109550531B (zh) * | 2019-01-28 | 2021-09-07 | 武汉纺织大学 | 一种磁性尺寸依赖的微流控芯片 |
CN110187104A (zh) * | 2019-06-13 | 2019-08-30 | 华中农业大学 | 基于生物正交反应的横向弛豫时间免疫传感器的制备方法、传感器及其应用 |
CN110187104B (zh) * | 2019-06-13 | 2021-09-14 | 华中农业大学 | 基于生物正交反应的横向弛豫时间免疫传感器的制备方法、传感器及其应用 |
CN110470688A (zh) * | 2019-07-29 | 2019-11-19 | 华中农业大学 | 一种纳米螯合筛介导的低场核磁共振免疫传感器及其应用 |
CN110499234A (zh) * | 2019-09-03 | 2019-11-26 | 浙江省人民医院 | 念珠菌检测设备及检测方法 |
CN110726841A (zh) * | 2019-10-21 | 2020-01-24 | 华中农业大学 | 一种基于酶促点击反应信号放大磁弛豫时间免疫传感器检测兽药残留的方法 |
CN111198267B (zh) * | 2020-01-09 | 2021-02-09 | 华中农业大学 | 一种磁弛豫时间免疫传感器磁信号探针及其应用 |
CN111198267A (zh) * | 2020-01-09 | 2020-05-26 | 华中农业大学 | 一种磁弛豫时间免疫传感器磁信号探针及其应用 |
CN111208287A (zh) * | 2020-01-16 | 2020-05-29 | 长沙理工大学 | 一种磁共振传感器的构建方法 |
CN111208287B (zh) * | 2020-01-16 | 2022-07-26 | 长沙理工大学 | 一种磁共振传感器的构建方法 |
CN112611868A (zh) * | 2020-12-18 | 2021-04-06 | 厦门大学 | 一种用于磁共振分析方法检测新型冠状病毒的探针及其制备方法 |
CN112964868A (zh) * | 2021-02-05 | 2021-06-15 | 华中农业大学 | 一种基于磁分离同时检测多种目标物的生化分析方法 |
CN112964868B (zh) * | 2021-02-05 | 2022-06-28 | 华中农业大学 | 一种基于磁分离同时检测多种目标物的生化分析方法 |
CN114966012A (zh) * | 2022-04-07 | 2022-08-30 | 宁波大学 | 基于超顺磁性二维材料的低场核磁共振均相免疫检测食源性致病菌的方法 |
CN114966012B (zh) * | 2022-04-07 | 2024-01-19 | 深圳万知达科技有限公司 | 基于超顺磁性二维材料的低场核磁共振均相免疫检测食源性致病菌的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN104614513B (zh) | 2016-05-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104614513B (zh) | 一种基于磁分离的弛豫时间免疫传感分析方法 | |
CN105308458B (zh) | 用于进行过敏症和自身免疫性疾病的诊断测定的自动化免疫分析*** | |
CN105181680B (zh) | 一种三聚氰胺的磁珠分离化学发光免疫检测方法 | |
CN103543260A (zh) | 一种基于磁分离-量子点免疫荧光传感检测生物大分子的方法及其试剂制备方法 | |
Chen et al. | One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads | |
CN110187104B (zh) | 基于生物正交反应的横向弛豫时间免疫传感器的制备方法、传感器及其应用 | |
CN103278521A (zh) | 一种检测生物大分子的磁共振免疫传感方法 | |
CN107389919A (zh) | 一种免标记荧光适配体传感器及其制备方法和应用 | |
CN108593641A (zh) | 一种定量检测全血样品中待测物质的试剂盒及方法 | |
ping Chen et al. | An immunosensor based on magnetic relaxation switch and polystyrene microparticle-induced immune multivalency enrichment system for the detection of Pantoea stewartii subsp. Stewartii | |
Sun et al. | A nonenzymatic optical immunoassay strategy for detection of Salmonella infection based on blue silica nanoparticles | |
CN104122285B (zh) | 一种基于磁性微珠的低场nmr稀有细胞检测方法 | |
CN102128934A (zh) | 流式微球检测人心肌钙蛋白t的方法 | |
CN102183666B (zh) | 高通量检测血清样本中十二种病原抗体的液相芯片及其制备方法和使用方法 | |
CN104122284A (zh) | 一种基于低场nmr检测磁珠浓度的方法及其应用 | |
JPS61128168A (ja) | 免疫分析方法 | |
CN101533021A (zh) | 一种新的检测血清样本中结核抗体的方法和产品 | |
CN101963616B (zh) | 一种检测血清样本中土拉抗体的蛋白悬浮芯片及其制备方法和使用方法 | |
CN202024999U (zh) | 一种检测东方马脑炎抗体的蛋白悬浮芯片*** | |
CN202024995U (zh) | 一种检测q热立克次体抗体的蛋白悬浮芯片*** | |
CN203133082U (zh) | 一种同步检测血清样本中多种抗体的蛋白悬浮芯片及*** | |
CN203133084U (zh) | 一种检测血清样本中蜱传脑炎抗体的蛋白悬浮芯片*** | |
CN104122286B (zh) | 一种基于低场nmr实时检测稀有细胞个数的方法 | |
CN104048953A (zh) | 一种痕量内毒素的快速检测方法和试剂盒 | |
CN203133085U (zh) | 一种检测血清中土拉抗体的蛋白悬浮芯片*** |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Sheng Xingyu Inventor after: Chen Yiping Inventor after: Cao Fengjing Inventor after: Zhang Xiaoqing Inventor after: Wu Jing Inventor after: Niu Yajing Inventor before: Sheng Xingyu Inventor before: Chen Yiping Inventor before: Cao Fengjing Inventor before: Zhang Xiaoqing Inventor before: Wu Jing Inventor before: Wang Zhuo Inventor before: Niu Yajing |
|
COR | Change of bibliographic data | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant |