CN104610049A - Method for extracting hydroxyl citric acid from garcinia cambogia - Google Patents
Method for extracting hydroxyl citric acid from garcinia cambogia Download PDFInfo
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- CN104610049A CN104610049A CN201510073788.3A CN201510073788A CN104610049A CN 104610049 A CN104610049 A CN 104610049A CN 201510073788 A CN201510073788 A CN 201510073788A CN 104610049 A CN104610049 A CN 104610049A
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- hydroxycitric acid
- gamboge
- citric acid
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- moving bed
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- ZMJBYMUCKBYSCP-UHFFFAOYSA-N Hydroxycitric acid Chemical compound OC(=O)C(O)C(O)(C(O)=O)CC(O)=O ZMJBYMUCKBYSCP-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 18
- 235000000885 Garcinia xanthochymus Nutrition 0.000 title abstract description 5
- 244000119461 Garcinia xanthochymus Species 0.000 title abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 13
- 238000009835 boiling Methods 0.000 claims abstract description 9
- 239000002904 solvent Substances 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims abstract description 5
- 239000001814 pectin Substances 0.000 claims abstract description 3
- 235000010987 pectin Nutrition 0.000 claims abstract description 3
- 229920001277 pectin Polymers 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 34
- 229940089491 hydroxycitric acid Drugs 0.000 claims description 34
- 239000011347 resin Substances 0.000 claims description 16
- 229920005989 resin Polymers 0.000 claims description 16
- 241000598860 Garcinia hanburyi Species 0.000 claims description 13
- 229940117709 gamboge Drugs 0.000 claims description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 238000000605 extraction Methods 0.000 claims description 9
- 238000010521 absorption reaction Methods 0.000 claims description 7
- 239000002594 sorbent Substances 0.000 claims description 7
- 239000003463 adsorbent Substances 0.000 claims description 5
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 238000003795 desorption Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000008213 purified water Substances 0.000 claims description 5
- 238000011084 recovery Methods 0.000 claims description 5
- 238000001179 sorption measurement Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 11
- 239000008367 deionised water Substances 0.000 description 6
- 229910021641 deionized water Inorganic materials 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 241000593508 Garcinia Species 0.000 description 5
- 239000000706 filtrate Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000003809 water extraction Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 102000011759 adducin Human genes 0.000 description 3
- 108010076723 adducin Proteins 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 2
- 108010036824 Citrate (pro-3S)-lyase Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- VBODVLQKFNEDRN-UHFFFAOYSA-N 1,2-dihydroxypropane-1,2,3-tricarboxylic acid;sodium Chemical compound [Na].OC(=O)C(O)C(O)(C(O)=O)CC(O)=O VBODVLQKFNEDRN-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 244000245602 Garcinia atroviridis Species 0.000 description 1
- 241000173371 Garcinia indica Species 0.000 description 1
- 241000134253 Lanka Species 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000005961 cardioprotection Effects 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000010829 isocratic elution Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 239000006225 natural substrate Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention aims to provide a method for extracting hydroxyl citric acid from garcinia cambogia. The method comprises the following steps: soaking arcinia cambogia into boiling water, extracting and concentrating the arcinia cambogia into extract A; concentrating to obtain a concentrated solution B after dissolving and filtering out pectin; separating and purifying through simulated moving bed chromatography to obtain a component C rich in hydroxyl citric acid; concentrating and recycling a solvent to obtain the hydroxyl citric acid with purity higher than 90%. According to the method for extracting hydroxyl citric acid disclosed by the invention, a simulated moving bed is adopted to separate and purify the hydroxyl citric acid, so that relevant separating parameters are determined, the steps are simple, and the product purity is relatively high. The hydroxyl citric acid obtained by the technical scheme disclosed by the invention has purity higher than 90%, and yield higher than 7%.
Description
Technical field
The present invention relates to a kind of method extracting Hydroxycitric acid from Fructus Resina garciniae.
Background technology
Hydroxycitric acid (hydroxycitric acid, HCA) be a kind of natural organic acids, be mainly present in abound with in the Indian subcontinent and Sri Lanka western part garcinia plant Garcinia cambogia, Garcinia indica, Garcinia atroviridis fruit shell in.Hydroxycitric acid is soluble in water, is slightly soluble in methyl alcohol, is insoluble in chlorine, sherwood oil and benzene.Hydroxycitric acid is more a lot of by force than natural substrate citric acid to the avidity of citrate lyase, can preferentially be combined with enzyme, suppresses the activity of citrate lyase, reduces body fat synthesis.Therefore, Hydroxycitric acid often by as the main functional component in natural slimming health product, for losing weight, reducing blood fat, depress appetite etc.Field of medicaments, the medicine that Hydroxycitric acid is also applied to preparing cardioprotection, improves the effects such as endurance.
About in prior art to the report of Hydroxycitric acid and derivative thereof, a kind of method of water extraction HCA from gamboge pericarp is disclosed in US5656316, extract is after anionite-exchange resin absorption, and through NaOH/KOH wash-out, elutriant obtains free acid through cation exchange resin column.Deacidite is reported to the separation condition of Hydroxycitric acid crude extract and effect in " ion exchange resin is to the separation and purification of Hydroxycitric acid in Fructus Resina garciniae " (Yi Xinru etc., " food and machinery ", in May, 2014, Vol.30, No.3).A kind of method preparing hydroxycitrate hydrochlorate is disclosed in US6147228.The method is that poach, filtration, filtrate in fresh or dry garcinia plant pericarp, with the process of 80 DEG C of NaOH aqueous solution, are obtained Hydroxycitric acid sodium.
Simulated moving bed chromatography technology introduces in liquid phantom preparing chromatogram by simulation moving-bed design philosophy, both the consumption having maintained liquid phantom preparing chromatogram is little, separation purity is high, and the advantage such as temperature-changeable operation, overcoming again usual liquid phantom preparing chromatogram can not the shortcoming of operate continuously, make it have separating power strong, equipment volume is little, and cost of investment is low, and is particularly conducive to the features such as the system of the high and difficult separation of heat of dissociation sensitivity.
Summary of the invention
The present invention aims to provide a kind of method can extracting Hydroxycitric acid efficiently from gamboge shell.
The involved in the present invention method extracting Hydroxycitric acid from gamboge shell, comprises the following steps:
(1) by the gamboge shell chip of drying, soak extraction in boiling water, extracting solution simmer down to extractum A;
(2) in medicinal extract, add ethanol or methyl alcohol dissolves, filter out pectin, vacuum concentration is concentrated solution B;
(3) concentrated solution B is carried out separating-purifying through simulated moving bed chromatography, obtain the component C being rich in Hydroxycitric acid;
(4) by component C concentration and recovery solvent, the Hydroxycitric acid of purity more than 90% is obtained.
In step of the present invention (1), extraction step can repeat to extract 3-5 time, and united extraction liquid, reconcentration is extractum A.
In step of the present invention (2), preferably add ethanol and dissolve, the mass concentration of concentrated solution B is 3.5g/L.
In step of the present invention (3), the sorbent material that simulated moving bed chromatography is filled is low-pole macroporous adsorbent resin, and water wash zone is purified water, and strippant is 50 ~ 80% ethanol, and its consumption is 1 ~ 3 times of resin volume; Resin absorption regenerated solvent is 95% ethanol or 2 ~ 4% aqueous sodium hydroxide solutions; Adsorption zone flow velocity 1 ~ 2BV/h; Water wash zone flow velocity 1 ~ 3BV/h; Desorption zone flow velocity 1 ~ 3BV/h; Breeding blanket flow velocity 2 ~ 3BV/h; Switching time is 700 ~ 800s; Temperature controls at 40 DEG C ~ 60 DEG C; Pressure-controlling is at 0.2MPa ~ 0.6MPa.
Further, in step of the present invention (3), switching time of simulated moving bed chromatography preferably 700.
Further, in step of the present invention (3), the sorbent material that simulated moving bed chromatography is filled is AB-8.
Although the separating-purifying that simulated moving bed technology can be applied to natural product or medicine has common recognition in the art, but because simulation moving-bed large constructional feature all concentrates into system by key elements such as continuous, the adverse current in chemical industry, rectifying, backflows, it is a multiple degree of freedom, dynamically, be mutually related non-linear system.Therefore, prediction, optimization and control for it are more complicated problems, and especially for a certain natural product kind, the prediction of separation parameter is a very complicated problem.Hydroxycitric acid extracting method of the present invention, adopts and simulation moving-bedly carries out separating-purifying to Hydroxycitric acid, determine relevant separation parameter, step is simple, product purity is higher, and the Hydroxycitric acid purity obtained with technical scheme of the present invention reaches more than 90%, and yield reaches more than 7%.
Embodiment
Embodiment 1
By the gamboge shell (Garcinia cambogia) of 500g drying 700mL boiling deionized water extraction 30min, then repeat extraction 2 times with 400mL boiling deionized water, merge whole extracting solution, simmer down to extractum A.In extractum A, add 95% ethanol dissolve, filter, filtrate enters simulated moving bed chromatography and is separated.Sorbent material is AB-8 macroporous adsorbent resin, and strippant is 50% aqueous ethanolic solution, and its consumption is 3 times of resin volume, water wash zone is purified water, resin absorption regenerated solvent is 95% ethanol, adsorption zone flow velocity 2BV/h, desorption zone flow velocity 3BV/h, water wash zone flow velocity 3BV/h, breeding blanket flow velocity 3BV/h, switching time is 700s, temperature 50 C, pressure 0.5MPa, obtains the component C being rich in Hydroxycitric acid.By component C concentration and recovery ethanol, obtain the Hydroxycitric acid 39g that purity is 91.3%, yield 7.8%.
Embodiment 2
By the gamboge shell (Garcinia cambogia) of 1kg drying 1500mL boiling deionized water extraction 40min, then repeat extraction 3 times with 800mL boiling deionized water, merge whole extracting solution, simmer down to extractum A.In extractum A, add 95% ethanol dissolve, filter, filtrate enters simulated moving bed chromatography and is separated.Sorbent material is AB-8 macroporous adsorbent resin, and strippant is 50% aqueous ethanolic solution, and its consumption is 3 times of resin volume, water wash zone is purified water, resin absorption regenerated solvent is 95% ethanol, adsorption zone flow velocity 2BV/h, desorption zone flow velocity 3BV/h, water wash zone flow velocity 3BV/h, breeding blanket flow velocity 3BV/h, switching time is 700s, temperature 50 C, pressure 0.5MPa, obtains the component C being rich in Hydroxycitric acid.By component C concentration and recovery ethanol, obtain the Hydroxycitric acid 81g that purity is 90.5%, yield 8.1%.
Embodiment 3
By the gamboge shell (Garcinia cambogia) of 2kg drying 3L boiling deionized water extraction 40min, then repeat extraction 3 times with 1500mL boiling deionized water, merge whole extracting solution, simmer down to extractum A.In extractum A, add 95% ethanol dissolve, filter, filtrate enters simulated moving bed chromatography and is separated.Sorbent material is AB-8 macroporous adsorbent resin, and strippant is 50% aqueous ethanolic solution, and its consumption is 3 times of resin volume, water wash zone is purified water, resin absorption regenerated solvent is 95% ethanol, adsorption zone flow velocity 2BV/h, desorption zone flow velocity 3BV/h, water wash zone flow velocity 3BV/h, breeding blanket flow velocity 3BV/h, switching time is 700s, temperature 50 C, pressure 0.5MPa, obtains the component C being rich in Hydroxycitric acid.By component C concentration and recovery ethanol, obtain the Hydroxycitric acid 172g that purity is 90.8%, yield 8.6%.
Embodiment 4
High performance liquid chromatography is adopted to detect Hydroxycitric acid in embodiment 1-3 and Hydroxycitric acid standard substance; Chromatographic condition: chromatographic column is C18 post, 250 × 4.6mm, 5 μm; Moving phase is 0.1% phosphoric acid water-methanol (99:1), isocratic elution; Volumetric flow rate 0.8mL/min; Determined wavelength 210mm; Column temperature 30 DEG C; Sampling volume 10 μ L; Standard substance external standard method is adopted to calculate the content of hydroxycitrate acid product; Analyze through HPLC, the Hydroxycitric acid obtained in embodiment 1-3 and standard substance all have maximum absorption band at retention time 5.3min place.
Claims (5)
1. from gamboge shell, extract a method for Hydroxycitric acid, it is characterized in that comprising the following steps:
(1) by the gamboge shell chip of drying, soak extraction in boiling water, extracting solution simmer down to extractum A;
(2) in medicinal extract, add ethanol or methyl alcohol dissolves, filter out pectin, vacuum concentration is concentrated solution B;
(3) concentrated solution B is carried out separating-purifying through simulated moving bed chromatography, obtain the component C being rich in Hydroxycitric acid; The sorbent material that simulated moving bed chromatography is filled is low-pole macroporous adsorbent resin, and water wash zone is purified water, and strippant is 50 ~ 80% ethanol, and its consumption is 1 ~ 3 times of resin volume; Resin absorption regenerated solvent is 95% ethanol or 2 ~ 4% aqueous sodium hydroxide solutions; Adsorption zone flow velocity 1 ~ 2BV/h; Water wash zone flow velocity 1 ~ 3BV/h; Desorption zone flow velocity 1 ~ 3BV/h; Breeding blanket flow velocity 2 ~ 3BV/h; Switching time is 700 ~ 800s; Temperature controls at 40 DEG C ~ 60 DEG C; Pressure-controlling is at 0.2MPa ~ 0.6MPa;
(4) by component C concentration and recovery solvent, the Hydroxycitric acid of purity more than 90% is obtained.
2. a kind of method extracting Hydroxycitric acid from gamboge shell as claimed in claim 1, is characterized in that: in described step (3), switching time of simulated moving bed chromatography preferably 700.
3. a kind of method extracting Hydroxycitric acid from gamboge shell as claimed in claim 1 or 2, is characterized in that: in described step (3), and the sorbent material that simulated moving bed chromatography is filled is AB-8.
4. a kind of method extracting Hydroxycitric acid from gamboge shell according to any one of claim 1-3, is characterized in that: in described step (2), add ethanol and dissolve, and the mass concentration of concentrated solution B is 3.5g/L.
5. a kind of method extracting Hydroxycitric acid from gamboge shell according to any one of claim 1-4, is characterized in that: in described step (1), and extraction step can repeat to extract 3-5 time, and united extraction liquid, reconcentration is extractum A.
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| CN201510073788.3A CN104610049B (en) | 2015-02-12 | 2015-02-12 | A kind of method extracting hydroxycitric acid from Fructus Resina garciniae |
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| CN201510073788.3A CN104610049B (en) | 2015-02-12 | 2015-02-12 | A kind of method extracting hydroxycitric acid from Fructus Resina garciniae |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106518653A (en) * | 2016-09-24 | 2017-03-22 | 合肥信达膜科技有限公司 | Membrane extraction process for hydroxycitric acid |
| CN107473958A (en) * | 2017-08-20 | 2017-12-15 | 合肥信达膜科技有限公司 | A kind of method that hydroxycitric acid is extracted from HCA |
| CN107927498A (en) * | 2017-11-28 | 2018-04-20 | 南京伊登生物医学科技有限公司 | A kind of beverage composition for treating dental erosion and its application for suppressing lithangiuria and forming and promote dissolve stone |
| CN110590543A (en) * | 2019-08-26 | 2019-12-20 | 湖南人文科技学院 | Method for extracting hydroxycitrate from lucidus fruit |
| CN115536521A (en) * | 2022-07-17 | 2022-12-30 | 李玉山 | Preparation method of hydroxycitric acid in lucuma tenuifolia fruits |
-
2015
- 2015-02-12 CN CN201510073788.3A patent/CN104610049B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| Y. S. LEWIS ET AL.: "(-)-HYDROXYCITRIC ACID-THE PRINCIPAL ACID IN THE FRUITS OF GARCINIA CAMBOGIA DESR.", 《PHYTOCHEMISTRY》, vol. 4, 31 December 1965 (1965-12-31), pages 619 - 625, XP 007903103, DOI: doi:10.1016/S0031-9422(00)86224-X * |
| 刘建奇: "柠檬酸模拟移动床色谱分离的模拟、优化和放大研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》, no. 03, 15 March 2009 (2009-03-15), pages 016 - 9 * |
| 吕裕斌: "模拟移动床分离天然产物的研究", 《中国优秀博硕士学位论文全文数据库 (博士) 工程科技Ⅰ辑》, no. 08, 15 August 2006 (2006-08-15), pages 016 - 2 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106518653A (en) * | 2016-09-24 | 2017-03-22 | 合肥信达膜科技有限公司 | Membrane extraction process for hydroxycitric acid |
| CN107473958A (en) * | 2017-08-20 | 2017-12-15 | 合肥信达膜科技有限公司 | A kind of method that hydroxycitric acid is extracted from HCA |
| CN107927498A (en) * | 2017-11-28 | 2018-04-20 | 南京伊登生物医学科技有限公司 | A kind of beverage composition for treating dental erosion and its application for suppressing lithangiuria and forming and promote dissolve stone |
| CN110590543A (en) * | 2019-08-26 | 2019-12-20 | 湖南人文科技学院 | Method for extracting hydroxycitrate from lucidus fruit |
| CN110590543B (en) * | 2019-08-26 | 2022-05-06 | 湖南人文科技学院 | Method for extracting hydroxycitrate from lucidus fruit |
| CN115536521A (en) * | 2022-07-17 | 2022-12-30 | 李玉山 | Preparation method of hydroxycitric acid in lucuma tenuifolia fruits |
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| CN104610049B (en) | 2016-08-31 |
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Denomination of invention: Method for extracting hydroxyl citric acid from garcinia cambogia Effective date of registration: 20200610 Granted publication date: 20160831 Pledgee: Bank of China Limited by Share Ltd. Hohhot Xinhua Branch Pledgor: INNER MONGOLIA CHANGHUI BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020150000026 |
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Denomination of invention: A method for extracting hydroxycitric acid from rattan yellow fruit Effective date of registration: 20210916 Granted publication date: 20160831 Pledgee: Tongliao Horqin sub branch of Bank of Inner Mongolia Co.,Ltd. Pledgor: INNER MONGOLIA CHANGHUI BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021150000062 |
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Date of cancellation: 20230423 Granted publication date: 20160831 Pledgee: Tongliao Horqin sub branch of Bank of Inner Mongolia Co.,Ltd. Pledgor: INNER MONGOLIA CHANGHUI BIOTECHNOLOGY CO.,LTD. Registration number: Y2021150000062 |
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Denomination of invention: A Method for Extracting Hydroxycitric Acid from Tenghuang Fruit Granted publication date: 20160831 Pledgee: China Construction Bank Tongliao Branch Pledgor: INNER MONGOLIA CHANGHUI BIOTECHNOLOGY CO.,LTD. Registration number: Y2024150000025 |