CN104605013A - Preparation method of thalictrum plant extract, thalictrum plant extract prepared according to preparation method, and application of thalictrum plant extract - Google Patents
Preparation method of thalictrum plant extract, thalictrum plant extract prepared according to preparation method, and application of thalictrum plant extract Download PDFInfo
- Publication number
- CN104605013A CN104605013A CN201510002789.9A CN201510002789A CN104605013A CN 104605013 A CN104605013 A CN 104605013A CN 201510002789 A CN201510002789 A CN 201510002789A CN 104605013 A CN104605013 A CN 104605013A
- Authority
- CN
- China
- Prior art keywords
- thalictrum
- extract
- preparation
- thalictrum spp
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000205578 Thalictrum Species 0.000 title claims abstract description 102
- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 239000000419 plant extract Substances 0.000 title abstract 9
- 244000052769 pathogen Species 0.000 claims abstract description 15
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 13
- 239000000284 extract Substances 0.000 claims description 123
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 98
- 241000675108 Citrus tangerina Species 0.000 claims description 45
- 238000000605 extraction Methods 0.000 claims description 32
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 13
- 239000012467 final product Substances 0.000 claims description 12
- 238000010298 pulverizing process Methods 0.000 claims description 12
- 230000006837 decompression Effects 0.000 claims description 10
- 238000004064 recycling Methods 0.000 claims description 10
- 238000003306 harvesting Methods 0.000 claims description 8
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000009973 maize Nutrition 0.000 claims description 7
- 241000122123 Penicillium italicum Species 0.000 claims description 6
- 239000008194 pharmaceutical composition Substances 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 4
- 241000946391 Alternaria citri Species 0.000 claims description 3
- 244000168141 Geotrichum candidum Species 0.000 claims description 3
- 235000017388 Geotrichum candidum Nutrition 0.000 claims description 3
- 239000000126 substance Substances 0.000 abstract description 13
- 230000002401 inhibitory effect Effects 0.000 abstract description 10
- 235000020971 citrus fruits Nutrition 0.000 abstract description 9
- 239000003814 drug Substances 0.000 abstract description 7
- 239000000203 mixture Substances 0.000 abstract description 6
- 241000207199 Citrus Species 0.000 abstract description 5
- 206010059866 Drug resistance Diseases 0.000 abstract 1
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 238000002481 ethanol extraction Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 27
- 239000000463 material Substances 0.000 description 18
- 230000000844 anti-bacterial effect Effects 0.000 description 15
- 238000012360 testing method Methods 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 201000010099 disease Diseases 0.000 description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 13
- 239000012153 distilled water Substances 0.000 description 11
- 239000001963 growth medium Substances 0.000 description 11
- 240000002319 Citrus sinensis Species 0.000 description 10
- 235000005976 Citrus sinensis Nutrition 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 10
- 239000000243 solution Substances 0.000 description 9
- 235000013399 edible fruits Nutrition 0.000 description 8
- 239000003899 bactericide agent Substances 0.000 description 7
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 7
- 230000000443 biocontrol Effects 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 230000001408 fungistatic effect Effects 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- -1 sucrose ester Chemical class 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 description 5
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 239000005820 Prochloraz Substances 0.000 description 5
- 229930006000 Sucrose Natural products 0.000 description 5
- 239000004330 calcium propionate Substances 0.000 description 5
- 235000010331 calcium propionate Nutrition 0.000 description 5
- 239000001768 carboxy methyl cellulose Substances 0.000 description 5
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 5
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 5
- 244000053095 fungal pathogen Species 0.000 description 5
- 235000011187 glycerol Nutrition 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- TVLSRXXIMLFWEO-UHFFFAOYSA-N prochloraz Chemical compound C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl TVLSRXXIMLFWEO-UHFFFAOYSA-N 0.000 description 5
- 239000005720 sucrose Substances 0.000 description 5
- 238000009792 diffusion process Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 230000003902 lesion Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 208000004429 Bacillary Dysentery Diseases 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 3
- 206010017915 Gastroenteritis shigella Diseases 0.000 description 3
- YWNUNVSMOKMJMG-UHFFFAOYSA-N O-methyl-thalsimidine Natural products O1C(C(=CC=2CCN=3)OC)=CC=2C=3CC(C=C2)=CC=C2OC(=C2)C(OC)=CC=C2CC2N(C)CCC3=C(OC)C(OC)=C(OC)C1=C23 YWNUNVSMOKMJMG-UHFFFAOYSA-N 0.000 description 3
- YWNUNVSMOKMJMG-LJAQVGFWSA-N Thalsimine Chemical compound O1C(C(=CC=2CCN=3)OC)=CC=2C=3CC(C=C2)=CC=C2OC(=C2)C(OC)=CC=C2C[C@@H]2N(C)CCC3=C(OC)C(OC)=C(OC)C1=C23 YWNUNVSMOKMJMG-LJAQVGFWSA-N 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 229930013930 alkaloid Natural products 0.000 description 3
- 238000004500 asepsis Methods 0.000 description 3
- RIOXQFHNBCKOKP-UHFFFAOYSA-N benomyl Chemical compound C1=CC=C2N(C(=O)NCCCC)C(NC(=O)OC)=NC2=C1 RIOXQFHNBCKOKP-UHFFFAOYSA-N 0.000 description 3
- MITFXPHMIHQXPI-UHFFFAOYSA-N benzoxaprofen Natural products N=1C2=CC(C(C(O)=O)C)=CC=C2OC=1C1=CC=C(Cl)C=C1 MITFXPHMIHQXPI-UHFFFAOYSA-N 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 201000005113 shigellosis Diseases 0.000 description 3
- PZBPKYOVPCNPJY-UHFFFAOYSA-N 1-[2-(allyloxy)-2-(2,4-dichlorophenyl)ethyl]imidazole Chemical compound ClC1=CC(Cl)=CC=C1C(OCC=C)CN1C=NC=C1 PZBPKYOVPCNPJY-UHFFFAOYSA-N 0.000 description 2
- BCPFWSWROVXGQA-UHFFFAOYSA-N 2-(2,4,4-trimethylpentan-2-yl)guanidine Chemical compound CC(C)(C)CC(C)(C)N=C(N)N BCPFWSWROVXGQA-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000005795 Imazalil Substances 0.000 description 2
- 206010023126 Jaundice Diseases 0.000 description 2
- 241001507673 Penicillium digitatum Species 0.000 description 2
- 241000613234 Thalictrum simplex Species 0.000 description 2
- 239000005842 Thiophanate-methyl Substances 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000000118 anti-neoplastic effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000711 cancerogenic effect Effects 0.000 description 2
- 239000006013 carbendazim Substances 0.000 description 2
- 231100000315 carcinogenic Toxicity 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 229960002125 enilconazole Drugs 0.000 description 2
- 239000000469 ethanolic extract Substances 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- 231100000206 health hazard Toxicity 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 230000010358 mechanical oscillation Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 235000010603 pastilles Nutrition 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 239000001508 potassium citrate Substances 0.000 description 2
- 229960002635 potassium citrate Drugs 0.000 description 2
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 2
- 235000011082 potassium citrates Nutrition 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- YFNCATAIYKQPOO-UHFFFAOYSA-N thiophanate Chemical compound CCOC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OCC YFNCATAIYKQPOO-UHFFFAOYSA-N 0.000 description 2
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 2
- 238000002137 ultrasound extraction Methods 0.000 description 2
- HYZJCKYKOHLVJF-UHFFFAOYSA-N 1H-benzimidazole Chemical compound C1=CC=C2NC=NC2=C1 HYZJCKYKOHLVJF-UHFFFAOYSA-N 0.000 description 1
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 206010010741 Conjunctivitis Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010017553 Furuncle Diseases 0.000 description 1
- GCLAFEGUXXHIFT-IWLDQSELSA-N Homoplantaginin Chemical compound C1=C2OC(C=3C=CC(O)=CC=3)=CC(=O)C2=C(O)C(OC)=C1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GCLAFEGUXXHIFT-IWLDQSELSA-N 0.000 description 1
- STECJAGHUSJQJN-GAUPFVANSA-N Hyoscine Natural products C1([C@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-GAUPFVANSA-N 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- STECJAGHUSJQJN-UHFFFAOYSA-N N-Methyl-scopolamin Natural products C1C(C2C3O2)N(C)C3CC1OC(=O)C(CO)C1=CC=CC=C1 STECJAGHUSJQJN-UHFFFAOYSA-N 0.000 description 1
- JYAPBWCAZXNDAR-UHFFFAOYSA-N Oxyacanthine Natural products COc1cc2CCN(C)C3Cc4ccc(Oc5cccc(CC6N(C)CCc7cc(OC)c(OC)c(Oc(c1)c23)c67)c5)c(O)c4 JYAPBWCAZXNDAR-UHFFFAOYSA-N 0.000 description 1
- 206010064458 Penicilliosis Diseases 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 241000593989 Scardinius erythrophthalmus Species 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 241000685608 Thalictrum cultratum Species 0.000 description 1
- 241000219271 Thalictrum foliolosum Species 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000006184 cosolvent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 229940124600 folk medicine Drugs 0.000 description 1
- 235000013569 fruit product Nutrition 0.000 description 1
- 244000000004 fungal plant pathogen Species 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 229950000289 guanoctine Drugs 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- RONFGUROBZGJKP-UHFFFAOYSA-N iminoctadine Chemical compound NC(N)=NCCCCCCCCNCCCCCCCCN=C(N)N RONFGUROBZGJKP-UHFFFAOYSA-N 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- HGNHIFJNOKGSKI-WDYNHAJCSA-N oxyacanthine Chemical compound C([C@H]1N(C)CCC=2C=C(C(OC=3C(OC)=C(OC)C=C4CCN(C)[C@H](C=34)CC3=CC=C(C=C3)O3)=CC=21)OC)C1=CC=C(O)C3=C1 HGNHIFJNOKGSKI-WDYNHAJCSA-N 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- WHHIPMZEDGBUCC-UHFFFAOYSA-N probenazole Chemical compound C1=CC=C2C(OCC=C)=NS(=O)(=O)C2=C1 WHHIPMZEDGBUCC-UHFFFAOYSA-N 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- STECJAGHUSJQJN-FWXGHANASA-N scopolamine Chemical compound C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-FWXGHANASA-N 0.000 description 1
- 229960002646 scopolamine Drugs 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000004308 thiabendazole Substances 0.000 description 1
- 235000010296 thiabendazole Nutrition 0.000 description 1
- WJCNZQLZVWNLKY-UHFFFAOYSA-N thiabendazole Chemical compound S1C=NC(C=2NC3=CC=CC=C3N=2)=C1 WJCNZQLZVWNLKY-UHFFFAOYSA-N 0.000 description 1
- 229960004546 thiabendazole Drugs 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000005760 tumorsuppression Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a preparation method of a thalictrum plant extract. The preparation method comprises ultrasonic-assisted ethanol extraction of thalictrum plant(s). Preferably, the thalictrum plant(s) are/is radix thalictri simplicis and/or radix et rhizoma thalictri. The invention further provides the thalictrum plant extract obtained according to the preparation method, and application of the thalictrum plant extract in preparation of a medicine composition used for inhibiting citrus postharvest pathogen(s). The thalictrum plant extract has a strong inhibitory effect on the citrus postharvest pathogen(s), and no chemical residue exists after the thalictrum plant extract is used for fresh citrus preservation, so that the thalictrum plant extract is harmless to health of human bodies, free from environmental pollution, and low in drug resistance causing possibility.
Description
Technical field
The invention belongs to fruit disease control and fresh-keeping field, be specifically related to a kind of to there is thalictrum spp extract of suppression oranges and tangerines postharvest pathogen effect and preparation method thereof and novelty teabag.
Background technology
Oranges and tangerines are the large trade fruit of the first in the world, are also the third-largest trade agricultural product.China's oranges and tangerines aboundresources, plant with a long history.Cultivated area and the output of current China oranges and tangerines all rank first in the world, and within 2013, oranges and tangerines cultivated area reaches more than 3,600 ten thousand mu, and total output is about more than 3,200 ten thousand tons, and Citrus Industry occupies very important status in China's agricultural economy.
Citrusfruit is organized delicate succulence and is rich in sugar, in the processes such as harvesting, storing and sale, mechanical damage easily occurs, and is subject to multiple infecting of disease fungus and produces postharvest disease after pericarp damage.The pathologic disease species that rear generation adopted by oranges and tangerines reaches more than 20 kinds, mainly contain penicilliosis that Italian mould (Penicillium italicum) causes and the green mould that Penicillium digitatum (Penicillium digitatum) causes, these diseases easily cause fruit rot to go bad, rotting rate is generally 10% ~ 30%, up to more than 40% ~ 50%, huge economic loss can be caused time serious.Other pathologic disease of oranges and tangerines also comprises black rot, sour rot, brown rot, brown Supreme Being maize ear rot, anthracnose and burnt maize ear rot etc.
For a long time, people mainly adopt chemical synthesis material to carry out fresh-keeping as antistaling agent to oranges and tangerines, and the chemical bactericide that different times uses is different.Borax, sorbic acid and its esters etc. are the oranges and tangerines anticorrisive agents used the earliest; The benzimidazole germicide comprising benomyl (Benomyl), thiophanate (Thiophanate), thiophanate methyl (Thiophanatemethyl), carbendazim (Carbendazim), probenazole (Thiabendazole) etc. is the second generation oranges and tangerines anticorrisive agent of the application seventies; The third generation oranges and tangerines anticorrisive agent of current use comprises imazalil (Imazalil), Guanoctine (Guazatine), Prochloraz (Prochloraz) etc.These chemical bactericides can kill or suppress the growth of pathogenic microorganism, have good corrosion-resistanting fresh-keeping effect.Because chemical bactericide can cause, medicament is residual, contaminated environment, and Long-Time Service causes oranges and tangerines to adopt the reasons such as rear germ develops immunity to drugs making the safety in utilization of chemical bactericide day by day obtain the concern of people.In addition, along with science and technology development, detect improving and the further investigation of chemical bactericide toxicity of analysis means, find that some chemical preservatives have the possibility of carcinogenic or potential carcinogenic, mutagenesis, teratogenesis.Some chemical bactericides have been forbidden by developed countries such as America and Europes or have limited use at present, and as benomyl and Guanoctine series bactericidal agent etc., oneself is prohibited use or restriction use in part fruit products by EPA.
In recent years, people start the research and development turning to natural plant antistaling agent.The advantages such as natural plant antistaling agent has efficiently, low toxicity, low-residual, pollution-free, use safety, adopt its process oranges and tangerines to carry out anti-corrosive fresh-keeping and will be subject to the popular welcome of consumer.In addition, medicine based on herbs is by multicomponent, and Mutiple Targets plays bacteriostasis, not easily develops immunity to drugs.Therefore, be necessary to find from natural plants, exploitation natural plant source antiseptic agent carries out oranges and tangerines fresh-keeping to reduce dependence to chemical agent.
Thalictrum is a genus under Ranunculaceae, is herbaceos perennial, is distributed in Southwestern China more, as to view and admire or medicinal.This platymiscium of China about have 29 kinds can hyoscine, many kinds all have heat-clearing, it is wet to control, sweating, only dysentery, control the effects such as hot eyes.Wherein, SHUIHUANGLIAN is the root of thalictrum spp T.simplex (Thalictrumsimplex L.var.brevipes Hara), and with all herbal medicine, cold in nature, bitter, can be used for jaundice, ascites, the diseases such as difficult urination; Washing eye is decocted in external application can control eye conjunctivitis.Horse hair is linked as the root and rhizome of thalictrum spp spun gold manyleaf meadowure rhizome and root (Thalictrum glandulosissimum), leafy grass of meadow rue (Thalictrum foliolosum DC.), Zhaotong grass of meadow rue (Thalictrum glandulosissimum var.chaotungense) and plateau grass of meadow rue (Thalictrumcultratum Wall.T.deciternatum Boivin) etc., cold in nature, bitter.Manyleaf meadowure rhizome and root has effect of heat-clearing and damp-drying drug, purging intense heat and detonicating, cures mainly diarrhea due to damp-heat, dysentery jaundice, cold, fever, measles, carbuncle swell the diseases such as sore furuncle, red eye, swell pain.
Chemical composition and pharmacology activity research prove, alkaloid is the main active of thalictrum spp, comprise the alkaloids compositions such as Tang Song Xining, Tang Songximingding, oxyacanthine, thalcimine, Homoplantaginin, there is the effects such as antiviral, Tumor suppression, immunological regulation.(Lee is holy each, Su Yuqing, etc.; The progress of alkaloids chemical composition and pharmacological action in thalictrum spp, " modern medicines and clinical ", 2014,20 (3): 312-321).Bibliographical information thalictrum spp also has effect (Deng Xiaodong, the Yang Zhao for the treatment of acute bacillary dysentery; The routine clinical analysis of thalictrum spp treatment bacillary dysentery 30, " contemporary Chinese medical journal ", 1996,6 (8): 65; Tian Huayong; The routine observation of curative effect of thalictrum spp treatment acute bacillary dysentery 300, " Hunan medical science ", 1986,3 (1): 20-21).The people such as Wang Haikun report bacteriostasis (Wang Haikun, the Li Shuyi of manyleaf meadowure rhizome and root to six kinds of pathogens such as staphylococcus aureus, epidermis coccus, beta hemolytic streptococcus, Escherichia coli, shigella dysenteriae, Pseudomonas aeruginosas; The antibacterial action of manyleaf meadowure rhizome and root, " China National folk medicine magazine ", 1999,8 (5): 289-290,310).The Chinese invention patent application of denomination of invention " manyleaf meadowure rhizome and root extract is preparing the application in antineoplastic " (publication number CN101306094A), discloses manyleaf meadowure rhizome and root water or extractive with organic solvent is preparing the application in antineoplastic.
Chinese invention patent application " a kind of method (publication number CN102516257A) extracting thalcimine from hard thalictrum spp ", discloses the method adopting supercritical carbon dioxide extracting to extract thalcimine from hard thalictrum spp.
Analyze above-mentioned literature search content, the purposes finding thalictrum spp extract is mainly antitumor and suppress some infection pathogens; Report is had no to the bacteriostasis of plant pathogenic fungi, is especially suppressing the system research in oranges and tangerines postharvest pathogenic fungi to have no report.Based on above-mentioned research background, inventor has carried out the preparation of thalictrum spp extract and prepared product thereof and has suppressed oranges and tangerines to adopt the research of rear mould germ, black rot and sour germ.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of preparation method of thalictrum spp extract and the thalictrum spp extract novelty teabag of preparation thereof are provided.The present invention is with natural Chinese herbal medicine for raw material, and the thalictrum spp extract of preparation to the fungistatic effect of oranges and tangerines postharvest pathogenic fungi obviously and without chemical residual, to human body without health hazard, environmentally safe, imply that good application prospect.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
A preparation method for thalictrum spp extract, adopts ultrasonic assistant to extract, take ethanol as Extraction solvent, comprise the steps:
The mass percent concentration adding thalictrum spp weight 10 ~ 40 times in described thalictrum spp is the ethanol of 40% ~ 100%, it is 40 ~ 60 DEG C at Extracting temperature, extraction time is 30min ~ 120min, and supersonic frequency is extract under the condition of 30 ~ 40KHz; Filter, reclaim ethanol, concentrated, to obtain final product.
In preparation method of the present invention, preferably, the weight of ethanol is 10 ~ 30 times of described thalictrum spp weight; Be more preferably 20 ~ 30 times.
Preferably, the mass percent concentration of ethanol is preferably 40% ~ 70%; Be more preferably 50% ~ 60%.
Preferably, ultrasonic assistant extraction time is preferably 60 ~ 120min, is more preferably 60 ~ 90min.
Preferably, the temperature that ultrasonic assistant extracts is 45 DEG C.
Also preferred, before alcohol extract, described thalictrum spp 40 ~ 50 DEG C oven dry, crosses 40 mesh sieves after pulverizing, gets minus sieve powder and carry out ultrasonic assistant extraction.
As a preferred detailed description of the invention, the invention provides a kind of preparation method of thalictrum spp extract, comprise the steps:
I. by thalictrum spp 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described thalictrum spp weight 20 ~ 30 times in the powder that step I obtains be 40% ~ 70% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 40 DEG C ~ 60 DEG C, extraction time is 60min ~ 120min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
As a preferred detailed description of the invention, the invention provides a kind of preparation method of thalictrum spp extract, comprise the steps:
I. by thalictrum spp 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described thalictrum spp weight 20 ~ 30 times in the powder that step I obtains be 50% ~ 60% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 45 DEG C, extraction time is 60 ~ 90min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
Preferably, thalictrum spp of the present invention is selected from SHUIHUANGLIAN and/or manyleaf meadowure rhizome and root.
As one preferred embodiment, the invention provides a kind of preparation method of SHUIHUANGLIAN extract, comprise the steps:
I. by SHUIHUANGLIAN 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described SHUIHUANGLIAN weight 20 times in the powder that step I obtains be 60% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 45 DEG C, and extraction time is 60min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
As another preferred implementation method, the invention provides a kind of preparation method of manyleaf meadowure rhizome and root extract, specifically comprise the steps:
I. by manyleaf meadowure rhizome and root 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described manyleaf meadowure rhizome and root weight 30 times in the powder that step I obtains be 50% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 45 DEG C, and extraction time is 90min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
Another object of the present invention, is the thalictrum spp extract providing above-mentioned preparation method to prepare; Particularly SHUIHUANGLIAN extract and manyleaf meadowure rhizome and root extract.
A further object of the invention, is to provide thalictrum spp or thalictrum spp extract for the preparation of the purposes in the pharmaceutical composition of control oranges and tangerines postharvest pathogen and/or the agent of oranges and tangerines post-harvest fresh-keeping; Preferably, described thalictrum spp extract is prepared by preparation method of the present invention.
Described oranges and tangerines postharvest pathogen is selected from Italian mould (Penicillium italicum), one or more in citric acid maize ear rot bacterium (Geotrichum candidum) and oranges and tangerines black rot (Alternaria citri).
Thus, the invention provides a kind of pharmaceutical composition preventing and treating oranges and tangerines postharvest pathogen, comprise the thalictrum spp extract that preparation method of the present invention prepares.
In addition, the present invention also provides the agent of a kind of oranges and tangerines post-harvest fresh-keeping, comprises the described thalictrum spp extract that preparation method of the present invention prepares.
Pharmaceutical composition of the present invention or the agent of oranges and tangerines post-harvest fresh-keeping, except described thalictrum spp extract, acceptable auxiliary material can also be comprised, such as be selected from the film forming agent such as carboxymethyl cellulose, shitosan, be selected from the cosolvent of glycerine, sucrose ester, tween etc., be selected from the anticorrisive agent of citric acid, calcium propionate, potassium sorbate or Sodium Benzoate etc., and suitable solvent, as water.
Ultrasonic wave has " cavitation effect ", the characteristic such as " mechanical oscillation " and " fuel factor ", " cavitation effect " can produce several thousand atmospheric pressures instantaneously, the micro-bubble of Extraction medium is compressed, bursts, the broken cell membrane extracting raw material, accelerate the stripping of natural medicinal component, " mechanical oscillation " and " fuel factor " further enhance the diffusion of stripping composition, make medicinal component reach concentration balance fast in Extraction medium, greatly can shorten extraction cost.And Extracting temperature is lower, avoids the destruction of long-time high-temperature water decoction to thalictrum spp chemical composition, improve the quality of products.
The extract that the present invention obtains for raw material with natural thalictrum spp, has very strong suppression Italy mould, the effect, particularly SHUIHUANGLIAN extract of oranges and tangerines black rot and sour germ and manyleaf meadowure rhizome and root extract.Pharmaceutical composition and the citrus preservative containing thalictrum spp extract of the present invention, without chemical residual, to human body without health hazard, environmentally safe, has good application prospect.
Accompanying drawing explanation
The photo display of Fig. 1 be in test example 1 thalictrum spp extract to the fungistatic effect of oranges and tangerines postharvest pathogen, arrow instruction be the edge of bacterial restrain; Wherein Figure 1A-1C shows the SHUIHUANGLIAN extract fungistatic effect to Italian mould, oranges and tangerines black rot and sour germ, and Fig. 1 D-1F shows the fungistatic effect of manyleaf meadowure rhizome and root extract to Italian mould, oranges and tangerines black rot and sour germ.
The photo display of Fig. 2 be that in test example 1, thalictrum spp extract inoculates the biocontrol effect of Italian mould to south, Jiangxi navel orange; Wherein Fig. 2 A shows SHUIHUANGLIAN extract inoculates Italian mould biocontrol effect to south, Jiangxi navel orange, and Fig. 2 B shows manyleaf meadowure rhizome and root extract inoculates Italian mould biocontrol effect to south, Jiangxi navel orange.
Detailed description of the invention
By concrete example given below, the invention will be further described, but it can not be interpreted as limitation of the invention.
Medicinal material, reagent, biomaterial that following embodiment is used, if no special instructions, all derive from commercial sources.
Embodiment 1 one kinds of SHUIHUANGLIAN extracts
Described SHUIHUANGLIAN extract is prepared as follows:
I. by SHUIHUANGLIAN 40 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the weight percent concentration adding described SHUIHUANGLIAN weight 20 times in the powder that step I obtains be 60% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 45 DEG C, and extraction time is 1 hour, and supersonic frequency is 30KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, obtains described SHUIHUANGLIAN extract.
Embodiment 2 one kinds of manyleaf meadowure rhizome and root extracts
Prepare as follows:
I. by manyleaf meadowure rhizome and root 40 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the weight percent concentration adding described manyleaf meadowure rhizome and root weight 30 times in the powder that step I obtains be 50% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 45 DEG C, and extraction time is 1 hour, and supersonic frequency is 30KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol to without alcohol taste, i.e. described manyleaf meadowure rhizome and root extract.
Test example 1 thalictrum spp extract is to the bacteriostasis of oranges and tangerines postharvest pathogenic fungi
Experiment material:
1. thalictrum spp extract:
SHUIHUANGLIAN extract prepared by embodiment 1, is mixed with distilled water the solution that concentration is 1g medicinal material/mL;
Manyleaf meadowure rhizome and root extract prepared by embodiment 2, is mixed with distilled water the solution that concentration is 1g medicinal material/mL.
2. strains tested: Italian mould (Penicillium italicum, ACCC30399) is from Chinese agriculture Culture Collection (ACCC); Citric acid maize ear rot bacterium (Geotrichum candidum) and oranges and tangerines black rot (Alternaria citri) laboratory are separated from south, the Jiangxi sick fruit of navel orange and obtain.
Experimental technique:
1, agar diffusion method measures thalictrum spp extract to the bacteriostasis of Italian mould
Get and be inoculated in test tube slant culture medium for examination bacterium, cultivate 2-3d for 30 DEG C, will wash in triangular flask for examination bacterium PDB nutrient solution by spore in test-tube culture medium, shake up, bead be broken up, and makes spore suspension, counts, make bacterial concentration reach 10 with blood counting chamber
6-10
7cfu/mL is for subsequent use.
Draw above-mentioned pityrosporion ovale suspension 5mL to be mixed into and to be cooled to about 50 DEG C PDA culture mediums, preparation band bacterium culture medium.Then place aseptic Oxford cup, respectively at adding thalictrum spp extract solution described in 200 μ L in the cup of Oxford, being placed in 28 DEG C of insulating boxs and cultivating 48h, right-angled intersection method measures the size of inhibition zone.Replication three times, calculates inhibition zone diameter mean value.
2, growth rate method measures the thalictrum spp extract bacteriostasis to citric acid maize ear rot bacterium and black rot
Under aseptic condition, with pure PDA culture medium for contrast, the each colony edge bacterium block for examination bacterium got and activated is beaten with diameter 6mm card punch, be transferred to containing in the PDA culture medium of described thalictrum spp extract and control medium respectively, each process in triplicate, and is placed in 28 DEG C of constant incubators and cultivates, and records each colony growth situation after 5d, and measure colony diameter by right-angled intersection method, calculate inhibiting rate.
Inhibiting rate (%)=100 × (contrast colony diameter-process colony diameter)/contrast colony diameter
3, doubling dilution measures minimum inhibitory concentration (MIC) and the MBC (MFC) of thalictrum spp extract
By ten concentration gradients between described thalictrum spp extract solution doubling dilution to 1.95mg medicinal material/mL ~ 1000mg medicinal material/mL, then the thalictrum spp extract solution of 10mL variable concentrations is drawn in the PDA culture medium do not melted containing 90mL, pour into after shaking up in flat board, make pastille culture medium, after culture medium solidifying, evenly be applied on pastille PDA culture medium by Italian mould pityrosporion ovale suspension with spread plate, each concentration in triplicate.And blank group (culture medium does not inoculate bacterium), bacterium liquid control group (culture medium inoculated bacterium but not containing thalictrum spp alcohol extract) are set.The incubator that test group and control group are placed in 28 DEG C is cultivated 2d, in test group with the culture dish of asepsis growth minimum containing concentration be its MIC, being continued to cultivate after 7d by the culture dish of all asepsis growths and observe, is its MFC with the culture dish of asepsis growth minimum containing concentration.
4, thalictrum spp extract is to the biocontrol effect of south, Jiangxi navel orange
By south, Jiangxi Navel Orange Fruits with after the process of 2%NaClO solution disinfection, cleaner with running water, dry for subsequent use.Stab a diameter 4mm with transfer needle at fruit waist, the wound of degree of depth 3mm, inoculate thalictrum spp extract and the 20 μ L 1 × 10 of 20 μ L variable concentrations with micropipettor
6cFU/mL Italy mould spore suspension, test point 3 process: 1. citrusfruit inoculates Italian mould spore suspension, as test blank; 2. Italian mould spore suspension is inoculated after thalictrum spp extract-treated citrusfruit wound; 3. Prochloraz positive controls.
Fruit is placed on pallet, puts bag film, and each process 9 fruits, repeat 3 times.Keep relative humidity about 95%, after 27 DEG C of cultivation 7d, investigation citrusfruit lesion diameter, according to following formulae discovery prevention effect.
Prevention effect (%)=100 × (control group lesion diameter-processed group lesion diameter)/control group lesion diameter
Experimental result
1, thalictrum spp extract is to the inhibitory action for examination pathogen
Result is see table 1 and Fig. 1.SHUIHUANGLIAN and manyleaf meadowure rhizome and root extract have significant bacteriostasis (respectively see Figure 1A and 1D) to Italian mould under the concentration of 1g medicinal material/mL, the size of the antibacterial circle diameter of SHUIHUANGLIAN is 47.4 ± 1.1mm, and the size of the antibacterial circle diameter of manyleaf meadowure rhizome and root is 53.1 ± 0.7mm.SHUIHUANGLIAN and the inhibiting rate of manyleaf meadowure rhizome and root extract to oranges and tangerines black rot and citric acid maize ear rot bacterium all reached for 100% (respectively see Figure 1B, Fig. 1 C, Fig. 1 E and Fig. 1 F).Simultaneously with the Prochlorazs of 1000 times of dilutions for positive control, find that the bacteriostasis of SHUIHUANGLIAN and manyleaf meadowure rhizome and root extract is suitable with 1000 times of Prochlorazs diluted.
Table 1 thalictrum spp extract is to the bacteriostasis of Italian mould
2, thalictrum spp alcohol extract suppresses MIC and MFC of Italian mould
SHUIHUANGLIAN is respectively 6.25mg medicinal material/mL and 50mg medicinal material/mL to Italian mould minimum inhibitory concentration (MIC) and MBC (MFC), MIC and MFC of manyleaf meadowure rhizome and root extract to Italian mould is respectively 6.25mg medicinal material/mL and 25mg medicinal material/mL.
3, thalictrum spp alcohol extract is to the biocontrol effect of south, Jiangxi navel orange
Result is see Fig. 2 and table 2.
Table 2 thalictrum spp alcohol extract is to the biocontrol effect of south, Jiangxi navel orange
The SHUIHUANGLIAN extract of 100mg medicinal material/mL and manyleaf meadowure rhizome and root extract are respectively 56.86% and 68.62% to south, the Jiangxi navel orange disease-controlling effect that Italian mould causes.Simultaneously with 500 times of dilutions and 1000 times of Prochlorazs diluted for positive control, find the Prochloraz strong (37.5%) of the prevention effect of two kinds of extracts of above-mentioned concentration than 1000 times of dilutions, the effect of SHUIHUANGLIAN extract is weaker than the prevention effect of the Prochloraz (68.1%) of 500 times of dilutions, and the effect of manyleaf meadowure rhizome and root is suitable with it.Show as linear relationship between the logarithm value of thalictrum spp extract concentrations and prevention effect, the linear equation of SHUIHUANGLIAN extract is Y=7.0159+1.8797X, as calculated EC
50for 84.6mg/mL; The linear equation of manyleaf meadowure rhizome and root extract is Y=6.7202+1.5029X, as calculated EC
50for 45.8mg/mL.
Be linked as the thalictrum spp of representative with SHUIHUANGLIAN and horse hair, its extract is adopted rear multiple pathogen to oranges and tangerines and is had very strong inhibitory action, effectively can prevent and treat the microbial oranges and tangerines postharvest disease of these cause of diseases.
The agent of embodiment 3 one kinds of oranges and tangerines post-harvest fresh-keepings
Prescription: SHUIHUANGLIAN extract 100mg prepared by embodiment 1, carboxymethyl cellulose 10g, potassium citrate 0.6g, sucrose ester 5g, glycerine 5g, calcium propionate 10g, surplus is distilled water, makes 1000mL altogether.
Preparation method: the carboxymethyl cellulose taking recipe quantity, adds the swelling 8h of appropriate distilled water, then adds distilled water stirring preparation cmc soln.Then add citric acid, sucrose ester, glycerine, calcium propionate and SHUIHUANGLIAN extract successively, with distilled water diluting to 1000mL, stir, to obtain final product.
The agent of embodiment 4 one kinds of oranges and tangerines post-harvest fresh-keepings
Prescription: manyleaf meadowure rhizome and root extract 100mg prepared by embodiment 2, carboxymethyl cellulose 10g, potassium citrate 0.6g, sucrose ester 5g, glycerine 5g, calcium propionate 10g, surplus is distilled water, makes 1000mL altogether.
Preparation method: the carboxymethyl cellulose taking recipe quantity, adds the swelling 8h of appropriate distilled water, then adds distilled water stirring preparation cmc soln.Then add citric acid, sucrose ester, glycerine, calcium propionate and manyleaf meadowure rhizome and root extract successively, with distilled water diluting to 1000mL, stir, to obtain final product.
Embodiment 5-19 SHUIHUANGLIAN extract
Prepare by the following method:
Step I ~ III and embodiment 1 with, but in Step II except Extracting temperature, each technological parameter is all different from embodiment 1, and the technological parameter of each embodiment refers to table 3.
The process conditions of the ultrasonic extraction of table 3 embodiment 5-19
Test example 2 SHUIHUANGLIAN extract is to the bacteriostasis of oranges and tangerines postharvest pathogenic fungi
Experiment material:
1. supplying reagent thing: SHUIHUANGLIAN extract prepared by embodiment 5-19, is the solution of 1g medicinal material/mL with distilled water compound concentration.
2. strains tested: Italian mould (Penicillium italicum, ACCC30399).
Experimental technique:
1, agar diffusion method measures SHUIHUANGLIAN extract to the bacteriostasis of Italian mould
With test example 1.
Experimental result: in table 4.
Table 4 SHUIHUANGLIAN extract is to the bacteriostasis of Italian mould
Embodiment | Antibacterial circle diameter | Embodiment | Antibacterial circle diameter | Embodiment | Antibacterial circle diameter |
5 | 36.17±0.55 | 10 | 40.17±0.55 | 15 | 47.67±0.52 |
6 | 38.83±0.41 | 11 | 37.50±0.41 | 16 | 43.27±0.75 |
7 | 37.00±0.98 | 12 | 36.50±0.98 | 17 | 40.17±0.82 |
8 | 34.17±0.52 | 13 | 44.17±0.52 | 18 | 38.67±0.52 |
9 | 39.00±0.52 | 14 | 45.83±0.63 | 19 | 37.00±0.52 |
Result of the test shows, the extracting factor described in embodiment of the present invention 5-19 extracts the SHUIHUANGLIAN extract obtained fungistatic effect, but the strongest with the ethanol extract effect of embodiment 15, is secondly embodiment 14,13 and 16.
Embodiment 20-34 manyleaf meadowure rhizome and root extract
Prepare by the following method:
Step I ~ III and embodiment 2 with, but in Step II except Extracting temperature, other technological parameter is all different from embodiment 2, the concrete technological parameter of each embodiment is in table 5.
The process conditions of the ultrasonic extraction of table 5 embodiment 20-34
Test example 3 manyleaf meadowure rhizome and root extract is to the bacteriostasis of oranges and tangerines postharvest pathogenic fungi
Experiment material:
1. supply reagent thing: manyleaf meadowure rhizome and root extract prepared by embodiment 20-34, concentration is the solution of 1g medicinal material/mL.
2. strains tested: Italian mould (Penicillium italicum, ACCC30399).
Experimental technique:
1, agar diffusion method measures manyleaf meadowure rhizome and root extract to the bacteriostasis of Italian mould
With test example 1.
Experimental result: in table 6.
Table 6 manyleaf meadowure rhizome and root extract is to the bacteriostasis of Italian mould
Embodiment | Antibacterial circle diameter | Embodiment | Antibacterial circle diameter | Embodiment | Antibacterial circle diameter |
20 | 38.33±0.89 | 25 | 41.17±1.03 | 30 | 53.83±0.89 |
21 | 40.67±0.82 | 26 | 43.50±1.05 | 31 | 53.17±0.82 |
22 | 41.00±0.98 | 27 | 41.33±1.03 | 32 | 47.67±0.75 |
23 | 41.67±1.03 | 28 | 52.67±0.75 | 33 | 45.00±0.89 |
24 | 40.50±0.89 | 29 | 55.50±1.36 | 34 | 41.67±0.75 |
Result of the test shows, the extracting factor described in embodiment of the present invention 20-34 extracts the manyleaf meadowure rhizome and root extract obtained fungistatic effect, but the strongest with the ethanol extract effect of embodiment 29, is secondly embodiment 30,31 and 28.
The result of compbined test example 2 and 3, optimizes a kind of preparation method of thalictrum spp extract, comprises the steps:
I. by thalictrum spp 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described thalictrum spp weight 20 ~ 30 times in the powder that step I obtains be 40% ~ 70% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 40 DEG C ~ 60 DEG C, extraction time is 60min ~ 120min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
The preparation method of the preferred thalictrum spp extract of the present invention, comprises the steps:
I. by thalictrum spp 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described thalictrum spp weight 20 ~ 30 times in the powder that step I obtains be 50% ~ 60% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 45 DEG C, extraction time is 60min ~ 90min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
In a word, the invention provides and a kind ofly rear multiple pathogen is adopted to oranges and tangerines there is very strong inhibiting thalictrum spp extract, effectively can prevent and treat the microbial oranges and tangerines postharvest disease of these cause of diseases.
Claims (10)
1. a preparation method for thalictrum spp extract, adopts ultrasonic assistant extractive technique, is Extraction solvent, comprises the steps: with ethanol
Be the ethanol of 40% ~ 100% by the mass percent concentration adding thalictrum spp weight 10 ~ 40 times in described thalictrum spp, it is 40 ~ 60 DEG C at Extracting temperature, extraction time is 30min ~ 120min, and supersonic frequency is extract under the condition of 30 ~ 40KHz; Filter, reclaim ethanol, concentrated, dry or moist, to obtain final product.
2. preparation method according to claim 1, is characterized in that: the weight of ethanol is described thalictrum spp weight 10 ~ 30 times, is preferably 20 ~ 30 times;
Preferably, the mass percent concentration of ethanol is 40% ~ 70%, is more preferably 50 ~ 60%;
Preferably, extraction time is 60 ~ 120min, is more preferably 60 ~ 90min;
Preferably, the temperature that ultrasonic assistant extracts is 45 DEG C.
3. preparation method according to claim 1 and 2, is characterized in that: before alcohol extract, described thalictrum spp 40 ~ 50 DEG C oven dry, crosses 40 mesh sieves, get minus sieve powder and carry out ultrasonic assistant extraction after pulverizing.
4. a preparation method for thalictrum spp extract, adopts ultrasonic assistant extractive technique, is Extraction solvent, comprises the steps: with ethanol
I. by thalictrum spp 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described thalictrum spp weight 20 ~ 30 times in the powder that step I obtains be 40% ~ 70% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 40 DEG C ~ 60 DEG C, extraction time is 60min ~ 120min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product;
Preferred technical scheme is:
I. by thalictrum spp 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described thalictrum spp weight 20 ~ 30 times in the powder that step I obtains be 50% ~ 60% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 45 DEG C, extraction time is 60 ~ 90min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
5. preparation method according to any one of claim 1 to 4, is characterized in that: described thalictrum spp is selected from SHUIHUANGLIAN and/or manyleaf meadowure rhizome and root.
6. the thalictrum spp extract that the preparation method according to any one of claim 1 to 5 prepares.
7. thalictrum spp or thalictrum spp extract are for the preparation of the purposes in the pharmaceutical composition of control oranges and tangerines postharvest pathogen and/or the agent of oranges and tangerines post-harvest fresh-keeping; Preferably, described thalictrum spp extract is prepared by the preparation method according to any one of claim 1 to 5.
8. purposes according to claim 7, it is characterized in that, described oranges and tangerines postharvest pathogen is selected from Italian mould (Penicillium italicum), one or more in citric acid maize ear rot bacterium (Geotrichum candidum) and oranges and tangerines black rot (Alternaria citri).
9. prevent and treat a pharmaceutical composition for oranges and tangerines postharvest pathogen, comprise thalictrum spp extract according to claim 6.
10. an oranges and tangerines post-harvest fresh-keeping agent, comprises thalictrum spp extract according to claim 6.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510002789.9A CN104605013B (en) | 2014-10-08 | 2015-01-05 | A kind of preparation method of thalictrum spp extract and its thalictrum spp extract and purposes of preparation |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410523396 | 2014-10-08 | ||
CN2014105233968 | 2014-10-08 | ||
CN201510002789.9A CN104605013B (en) | 2014-10-08 | 2015-01-05 | A kind of preparation method of thalictrum spp extract and its thalictrum spp extract and purposes of preparation |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104605013A true CN104605013A (en) | 2015-05-13 |
CN104605013B CN104605013B (en) | 2017-12-01 |
Family
ID=53139885
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510002789.9A Expired - Fee Related CN104605013B (en) | 2014-10-08 | 2015-01-05 | A kind of preparation method of thalictrum spp extract and its thalictrum spp extract and purposes of preparation |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104605013B (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105496872A (en) * | 2015-12-25 | 2016-04-20 | 肇庆迪彩日化科技有限公司 | Sun-blocking composition and daily chemical product with sun-blocking effect |
CN105613723A (en) * | 2016-03-04 | 2016-06-01 | 珠海真绿色技术有限公司 | Fruit and vegetable preservative and preparation method thereof |
CN106106550A (en) * | 2016-07-13 | 2016-11-16 | 彭水县渝宏清脆李种植股份合作社 | A kind of compositions preventing and treating blue or green crisp plum red leaf spot |
KR20190052256A (en) * | 2017-11-08 | 2019-05-16 | 대한민국(농촌진흥청장) | Antimicrobial composition comprising Thalictrum uchiyamai extracts and fractions for cladobotryum mycophilum and uses thereof |
KR20190052254A (en) * | 2017-11-08 | 2019-05-16 | 대한민국(농촌진흥청장) | Antimicrobial composition comprising Thalictrum uchiyamai extracts and fractions for Blue Mold, and uses thereof |
CN113200989A (en) * | 2021-05-18 | 2021-08-03 | 云南民族大学 | Preparation method and application of chromone alkaloid compound |
CN113234084A (en) * | 2021-05-18 | 2021-08-10 | 云南民族大学 | Compound with anti-rotavirus activity in agrimony and thalictrum aquilegifolium and preparation method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1232621A (en) * | 1998-04-22 | 1999-10-27 | 黎光海 | Longan and litchi fresh-keeping agent and its compounding process |
CN1457653A (en) * | 2002-05-15 | 2003-11-26 | *** | Fresh-ness preserving agent for vegetable and fruit |
CN102018024A (en) * | 2010-10-27 | 2011-04-20 | 陈金印 | Method for preparing and using plant extract for inhibiting orange post-harvest pathogenic bacteria |
CN103222486A (en) * | 2013-05-23 | 2013-07-31 | 中国科学院成都生物研究所 | Plant extract with inhibiting effect on orange pathogenic bacteria as well as preparation method and application thereof |
CN103404537A (en) * | 2013-08-23 | 2013-11-27 | 江西农业大学 | Preparation method of cassia twig extractive, prepared cassia twig extractive and application of prepared cassia twig extractive |
-
2015
- 2015-01-05 CN CN201510002789.9A patent/CN104605013B/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1232621A (en) * | 1998-04-22 | 1999-10-27 | 黎光海 | Longan and litchi fresh-keeping agent and its compounding process |
CN1457653A (en) * | 2002-05-15 | 2003-11-26 | *** | Fresh-ness preserving agent for vegetable and fruit |
CN102018024A (en) * | 2010-10-27 | 2011-04-20 | 陈金印 | Method for preparing and using plant extract for inhibiting orange post-harvest pathogenic bacteria |
CN103222486A (en) * | 2013-05-23 | 2013-07-31 | 中国科学院成都生物研究所 | Plant extract with inhibiting effect on orange pathogenic bacteria as well as preparation method and application thereof |
CN103404537A (en) * | 2013-08-23 | 2013-11-27 | 江西农业大学 | Preparation method of cassia twig extractive, prepared cassia twig extractive and application of prepared cassia twig extractive |
Non-Patent Citations (1)
Title |
---|
万春鹏等: "桂枝抑制柑橘采后两种病原菌活性物质研究", 《江西农业大学学报》 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105496872A (en) * | 2015-12-25 | 2016-04-20 | 肇庆迪彩日化科技有限公司 | Sun-blocking composition and daily chemical product with sun-blocking effect |
CN105496872B (en) * | 2015-12-25 | 2019-09-13 | 肇庆迪彩日化科技有限公司 | A kind of sunscreen composition and the daily chemical product with sun-proof function |
CN105613723A (en) * | 2016-03-04 | 2016-06-01 | 珠海真绿色技术有限公司 | Fruit and vegetable preservative and preparation method thereof |
CN106106550A (en) * | 2016-07-13 | 2016-11-16 | 彭水县渝宏清脆李种植股份合作社 | A kind of compositions preventing and treating blue or green crisp plum red leaf spot |
KR20190052256A (en) * | 2017-11-08 | 2019-05-16 | 대한민국(농촌진흥청장) | Antimicrobial composition comprising Thalictrum uchiyamai extracts and fractions for cladobotryum mycophilum and uses thereof |
KR20190052254A (en) * | 2017-11-08 | 2019-05-16 | 대한민국(농촌진흥청장) | Antimicrobial composition comprising Thalictrum uchiyamai extracts and fractions for Blue Mold, and uses thereof |
KR102069118B1 (en) * | 2017-11-08 | 2020-01-22 | 대한민국 | Antimicrobial composition comprising Thalictrum uchiyamai extracts and fractions for Blue Mold, and uses thereof |
KR102069114B1 (en) * | 2017-11-08 | 2020-01-22 | 대한민국 | Antimicrobial composition comprising Thalictrum uchiyamai extracts and fractions for cladobotryum mycophilum and uses thereof |
CN113200989A (en) * | 2021-05-18 | 2021-08-03 | 云南民族大学 | Preparation method and application of chromone alkaloid compound |
CN113234084A (en) * | 2021-05-18 | 2021-08-10 | 云南民族大学 | Compound with anti-rotavirus activity in agrimony and thalictrum aquilegifolium and preparation method thereof |
CN113234084B (en) * | 2021-05-18 | 2023-05-05 | 云南民族大学 | Compound with anti-rotavirus activity in Meadowrue of Crane celebration and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
CN104605013B (en) | 2017-12-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104605013B (en) | A kind of preparation method of thalictrum spp extract and its thalictrum spp extract and purposes of preparation | |
CN102669191B (en) | Plant bacteriostatic composition and application thereof in cosmetics | |
CN103404537B (en) | A kind of preparation method of Ramulus Cinnamomi extract and the Ramulus Cinnamomi extract of preparation thereof and purposes | |
CN104255823B (en) | A kind of plant bacteriostatic composition and its application in liquid detergent | |
Ibrahim et al. | Antibacterial activity of nutmeg (Myristica fragrans) seed extracts against some pathogenic bacteria | |
CN104542740A (en) | Traditional Chinese medicine disinfectant for sterilization and disinfection of clinical laboratory instruments and preparation method of traditional Chinese medicine disinfectant | |
CN103329949A (en) | Disinfectant for clinical laboratory and preparation method of disinfectant | |
CN106619380A (en) | Anti-acne essence | |
CN104585244A (en) | Method for preparing radix fici simplicissimae extract, radix fici simplicissimae extract and application of extract | |
CN103053738A (en) | Tea-processing herba cistanche and preparation method thereof | |
CN103931676A (en) | Application of Chinese toon extract | |
CN105148142B (en) | Disposable hygienic sterilizing article containing antibacterial plant extract and preparation method thereof | |
CN104585306B (en) | A kind of preparation method of Rhizoma Smilacis Glabrae extract and its Rhizoma Smilacis Glabrae extract of preparation and purposes | |
KR101873297B1 (en) | Cosmetic compositon for removing heavy metal or fine dust containing extracts of ficus religosa | |
CN110327259B (en) | Wet tissue for children | |
CN112772681B (en) | Biological compound disinfectant and preparation method thereof | |
CN104257826A (en) | Traditional Chinese medicine pycnonotus sinensis fermented granule for livestock and poultry and preparation method of traditional Chinese medicine pycnonotus sinensis fermented granule | |
CN104585305B (en) | A kind of preparation method of Radix Cynanchi Atrati extract and its Radix Cynanchi Atrati extract of preparation and purposes | |
CN111904903B (en) | Oral cavity gargle and preparation method thereof | |
CN107519328A (en) | A kind of radix tetrastigme antimicrobial fluid and preparation method thereof | |
CN101449804A (en) | Extraction method of Mongolian snakegourd effective ingredient | |
CN115531464B (en) | Traditional Chinese medicine composition for resisting mycoplasma caprae pneumonia as well as preparation method and application thereof | |
CN106797965A (en) | Application of the short-tube lycoris flower extract in preparing treatment or preventing mycotic medicine | |
CN106619887B (en) | Compound traditional Chinese medicine preparation for inhibiting and killing schbert unicellular bacteria, preparation method and application thereof | |
CN104857116B (en) | Application of the Chinese medicine garden burnet in preparing antifungal medicine synergist |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
EXSB | Decision made by sipo to initiate substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171201 |
|
CF01 | Termination of patent right due to non-payment of annual fee |