CN104597259A - Antihuman-globulin blood matching detection card with different formulas at primary side and secondary side - Google Patents

Antihuman-globulin blood matching detection card with different formulas at primary side and secondary side Download PDF

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Publication number
CN104597259A
CN104597259A CN201510000984.8A CN201510000984A CN104597259A CN 104597259 A CN104597259 A CN 104597259A CN 201510000984 A CN201510000984 A CN 201510000984A CN 104597259 A CN104597259 A CN 104597259A
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Prior art keywords
pipes
pipe
primary
antihuman
secondary side
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CN201510000984.8A
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许明安
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Individual
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Priority to CN201510000984.8A priority Critical patent/CN104597259A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention discloses an antihuman-globulin blood matching detection card with different formulas at the primary side and the secondary side. The antihuman-globulin blood matching detection card comprises 6 micro-column pipes or 8 micro-column pipes for labeling the primary side and the secondary side, wherein the 6 micro-column pipes are pipes 1-6 in sequence from left to right, i.e., the pipe 1, the pipe 3 and the pipe 5 are the primary-side pipes, and the pipe 2, the pipe 4 and the pipe 6 are secondary-side pipes; the primary-side pipes are used for detecting irregular antibodies and complement component C3d; the secondary-side pipes are used for detecting the irregular antibodies; the primary-side pipes contain anti-IgG, anti-C3d and 0.1%-0.5% of NaCl; and the secondary-side pipes contain anti-IgG and 0.2-0.6% of NaCl. The antihuman-globulin blood matching detection card disclosed by the invention has the advantages that the primary-side pipes and the secondary-side pipes adopt different formulas, so that the specificity and the sensitivity of blood-matching detection are improved and the problems of non-specificity, false positive and false negative generated in blood matching of the primary side and the secondary side in the existing antihuman-globulin detection technology are solved.

Description

The antihuman globulin match test card that a kind of primary and secondary side is different
Technical field
The present invention relates to blood transfusion medical domain, the antihuman globulin formula match test card that particularly a kind of primary and secondary side is different.
Background technology
Several technical scheme is had to carry out match at present: salt solution match, polybrene match, the match of test tube method anti-human ball, the anti-human ball match of LISS/Coombs card and the match of anti-igg anti-human ball card.In cross matching, the test of microtrabeculae centrifugation technique, anti-human ball card may be used for detecting irregular antibody or incomplete antibody in sample.Wide spectrum anti-human ball card LISS/Coombs card is a product of the anti-human ball cartoon in market, is mainly used to do Antibody screening.The anti-human ball card of anti-igg is mainly used to do neonatal hemolytic disease research, and irregular antibody detects.There is no specific match card product at present, non-specific aggegation that clinical sample causes can not be solved or susceptibility causes undetected not.If with the match of wide spectrum anti-human ball card LISS/Coombs card, irregular antibody and complement activation composition can be detected.For minor cross-match pipe, patient's cell sample is often because patient's medicine and causes for pathological produce complement activation composition, instead of the complement activation that irregular antibody causes.At this moment the positive of secondary side is much false positive, is not the reaction caused by irregular antibody, is nonspecific reaction.If do blood mathcing test with anti-human ball card anti-igg card, can irregular antibody be detected, but the complement activation that irregular antibody causes can not be detected.Clinical research shows that irregular antibody can activating complement composition, does not detect complement in major cross-match, can undetected irregular antibody.
Summary of the invention
For above-mentioned technical matters, the present invention discloses the antihuman globulin match test card of a kind of primary and secondary side different formulations, comprise: 6 microtrabeculae pipes or 8 microtrabeculae pipes, be followed successively by No. 1-6 pipe from left to right, wherein No. 1, No. 3, No. 5 pipes are master pipe, No. 2, No. 4, No. 6 Guan Weici side pipes, described master pipe is for detecting irregular antibody and complement component C3 D; Described side pipe is for detecting irregular antibody; Described master pipe includes the NaCl of anti-igg, anti-C3d and 0.1%-0.5%, and described side pipe includes the NaCl of anti-igg and 0.2%-0.6%.
Described microtrabeculae pipe is poured into by gel or beaded glass material and is formed to plastic tube.
The invention has the beneficial effects as follows: master pipe and time side pipe of match test card of the present invention adopt different formulas, improve specificity and sensitivity that match detects, non-specific, the false positive solving that master minor cross-match in existing anti-human playing skill art occurs and false negative; The complement activation adopting master detection irregular antibody and irregular antibody to cause, improves sensitivity, is better than the match card only detecting irregular antibody; Irregular antibody is only detected in secondary side, does not detect complement component, avoids the false positive that the non-specific complement of patient's sample own cells causes.
Embodiment
The present invention is described in further detail below, can implement according to this with reference to instructions word to make those skilled in the art.
The present invention discloses the antihuman globulin match test card of a kind of primary and secondary side different formulations, comprise: 6 microtrabeculae pipes or 8 microtrabeculae pipes, mark master time side, be followed successively by No. 1-6 pipe from left to right, wherein No. 1, No. 3, No. 5 pipes are master pipe, No. 2, No. 4, No. 6 Guan Weici side pipes, described master pipe is for detecting irregular antibody and complement component C3 D; Described side pipe is for detecting irregular antibody; Described master pipe includes the NaCl of anti-igg, anti-C3d and 0.1%-0.5%, and described side pipe includes the NaCl of anti-igg, anti-C3d and 0.2%-0.6%.Described microtrabeculae pipe is poured into by gel, beaded glass or other particulate material and is formed to plastic tube.Master pipe and time side pipe of described match test card adopt different formulas, improve specificity and the sensitivity of match detection, solve the non-specific and false negative that in existing anti-human playing skill art, master minor cross-match occurs; The complement activation adopting master detection irregular antibody and irregular antibody to cause, improves sensitivity, is better than the match card only detecting irregular antibody; Irregular antibody is only detected in secondary side, does not detect complement component, avoids the false positive that the non-specific complement of patient's sample own cells causes.
Although embodiment of the present invention are open as above, but it is not restricted to listed in instructions and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the word described.

Claims (2)

1. the antihuman globulin test card of a primary and secondary side different formulations, it is characterized in that, comprise: 6 or 8 microtrabeculae pipes, mark primary and secondary side, be followed successively by No. 1-6 pipe from left to right, wherein No. 1, No. 3, No. 5 pipes are master pipe, No. 2, No. 4, No. 6 Guan Weici side pipes, and described master pipe is for detecting irregular antibody and complement component C3 D; Described side pipe is for detecting irregular antibody; Described master pipe includes the NaCl of anti-igg, anti-C3d and 0.1%-0.5%, and described side pipe includes the NaCl of anti-igg and 0.2%-0.6%.
2. the antihuman globulin match test card of primary and secondary side according to claim 1 different formulations, it is characterized in that: described microtrabeculae pipe is poured into by gel or beaded glass material and formed to plastic tube, mark primary and secondary side, containing the anti-human ball testing tube of different formulations in an anti-human ball card.
CN201510000984.8A 2015-01-04 2015-01-04 Antihuman-globulin blood matching detection card with different formulas at primary side and secondary side Pending CN104597259A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510000984.8A CN104597259A (en) 2015-01-04 2015-01-04 Antihuman-globulin blood matching detection card with different formulas at primary side and secondary side

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510000984.8A CN104597259A (en) 2015-01-04 2015-01-04 Antihuman-globulin blood matching detection card with different formulas at primary side and secondary side

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108680756A (en) * 2018-05-21 2018-10-19 中国科学院苏州生物医学工程技术研究所 A kind of incomplete antibody detection kit and detection method
CN108693355A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Antihuman globulin detection reagent card and preparation method thereof
CN108693346A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Lectin A1 Blood grouping reagent cards and preparation method thereof
CN108693361A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Positive and negative group stereotypy reagent card of zirconium oxide microballon abo blood group and preparation method thereof
CN108693359A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Zirconium oxide microballon Rh blood grouping reagent cards and preparation method thereof
CN108693360A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Zirconium oxide microballon rare blood type detection reagent card and preparation method thereof

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GB2117514A (en) * 1982-03-29 1983-10-12 East Anglian Regional Health Polyvalent antiglobulin reagent
US5213963A (en) * 1988-10-12 1993-05-25 Biotest Aktiengesellschaft Procedure for finding and identifying red cell antibodies by means of the solid phase method
US5863802A (en) * 1987-08-24 1999-01-26 Stiftung Fur Diagnostische Forschung Method and test kit detecting antigens and/or antibodies
CN101718785A (en) * 2009-11-25 2010-06-02 江阴力博医药生物技术有限公司 Preparation method of direct antihuman globulin reagent card
CN102507961A (en) * 2011-10-21 2012-06-20 江苏中盛医学诊断试剂有限公司 Newborn ABO/Rh blood grouping reagent card and preparation method thereof
CN102692510A (en) * 2012-06-07 2012-09-26 北京金豪制药股份有限公司 Broad spectrum antihuman globulin reagent assay card and preparation thereof
CN103424544A (en) * 2008-01-30 2013-12-04 奥索临床诊断有限公司 Immunodiagnostic test cards having indicating indicia

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2117514A (en) * 1982-03-29 1983-10-12 East Anglian Regional Health Polyvalent antiglobulin reagent
US5863802A (en) * 1987-08-24 1999-01-26 Stiftung Fur Diagnostische Forschung Method and test kit detecting antigens and/or antibodies
US5213963A (en) * 1988-10-12 1993-05-25 Biotest Aktiengesellschaft Procedure for finding and identifying red cell antibodies by means of the solid phase method
CN103424544A (en) * 2008-01-30 2013-12-04 奥索临床诊断有限公司 Immunodiagnostic test cards having indicating indicia
CN101718785A (en) * 2009-11-25 2010-06-02 江阴力博医药生物技术有限公司 Preparation method of direct antihuman globulin reagent card
CN102507961A (en) * 2011-10-21 2012-06-20 江苏中盛医学诊断试剂有限公司 Newborn ABO/Rh blood grouping reagent card and preparation method thereof
CN102692510A (en) * 2012-06-07 2012-09-26 北京金豪制药股份有限公司 Broad spectrum antihuman globulin reagent assay card and preparation thereof

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108693355A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Antihuman globulin detection reagent card and preparation method thereof
CN108693346A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Lectin A1 Blood grouping reagent cards and preparation method thereof
CN108693361A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Positive and negative group stereotypy reagent card of zirconium oxide microballon abo blood group and preparation method thereof
CN108693359A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Zirconium oxide microballon Rh blood grouping reagent cards and preparation method thereof
CN108693360A (en) * 2017-09-25 2018-10-23 广东睿碁生物科技有限公司 Zirconium oxide microballon rare blood type detection reagent card and preparation method thereof
CN108693361B (en) * 2017-09-25 2021-03-09 广东睿碁生物科技有限公司 Zirconia micro-bead ABO blood type positive and negative sizing reagent card and preparation method thereof
CN108693360B (en) * 2017-09-25 2021-03-09 广东睿碁生物科技有限公司 Zirconia micro-bead rare blood type detection reagent card and preparation method thereof
CN108693346B (en) * 2017-09-25 2021-03-09 广东睿碁生物科技有限公司 Lectin A1 blood group detection reagent card and preparation method thereof
CN108680756A (en) * 2018-05-21 2018-10-19 中国科学院苏州生物医学工程技术研究所 A kind of incomplete antibody detection kit and detection method

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Application publication date: 20150506