CN104591402B - A kind of syntrophism fatty acid oxidation bacterium and the construction method for producing electricity bacterium dominant microflora - Google Patents
A kind of syntrophism fatty acid oxidation bacterium and the construction method for producing electricity bacterium dominant microflora Download PDFInfo
- Publication number
- CN104591402B CN104591402B CN201510006685.5A CN201510006685A CN104591402B CN 104591402 B CN104591402 B CN 104591402B CN 201510006685 A CN201510006685 A CN 201510006685A CN 104591402 B CN104591402 B CN 104591402B
- Authority
- CN
- China
- Prior art keywords
- bacterium
- syntrophism
- fatty acid
- acid oxidation
- construction method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Landscapes
- Water Treatment By Electricity Or Magnetism (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of syntrophism fatty acid oxidation bacterium and the construction method for producing electricity bacterium dominant microflora, syntrophism fatty acid oxidation bacterium is using volatility organic aliphatic acid for substrate is metabolized, its fermentation end productses acetic acid alive catalysis outside under by electricity production bacterium further with, realize the step degraded of organic matter, improve the treatment efficiency of high concentrated organic wastewater and the stability of system, the yield of hydrogen is improve simultaneously, reaches the unification of pollution treatment and production capacity.Construction method is comprised the following steps：The enrichment of syntrophism fatty acid oxidation bacterium, produces electricity the enrichment of bacterium, and two kinds be enriched withs successfully flora and are added to the process such as removal volatility organic aliphatic acid in MEC reactors.It is flexible, stable with simple structure, operation, without adjusting basicity, the low advantage of operating cost.
Description
Technical field
The present invention relates to Sewage Biological Treatment and its reuse field, and in particular to a kind of syntrophism fatty acid oxidation bacterium and electricity production
The construction method of bacterium dominant microflora.
Background technology
Anaerobic Microbiological Treatment Technology is widely used in the place of high concentrated organic wastewater by its economy, Safety and Environmental Protection
Among reason, and it is increasingly becoming first-selected technique.
The completion of larger molecular organicses anaerobic degradation process in traditional sense, is in acidogenic fermentation bacterium, syntrophism aliphatic acid
It is gradually completing under the mutual cooperation of the different microorganisms monoid such as oxidation bacteria and methanogen.These microbe groups are given birth in nutrition
It is a kind of " biological chain " relation on state position：Acidogenic fermentation bacterium provides available substrate volatile acid for syntrophism fatty acid oxidation bacterium
（VFAs）, the metabolite acetic acid and H of syntrophism fatty acid oxidation bacterium2Then utilized by methanogen.Syntrophism fatty acid oxidation bacterium
Function niche between acidogenic fermentation bacterium and methanogen, play the key effect formed a connecting link, be the of anaerobic metabolism
One rate-limiting step.But due to the limitation of thermokinetics, the metabolic response of syntrophism fatty acid oxidation bacterium spontaneous cannot be carried out, it is necessary to according to
Pulled by biting the metabolism of hydrogen bacterium (methanogen, homoacetogenic bacteria, sulfate reducing bacteria etc.), in traditional anaerobic technique
Mainly by being realized with methanogen syntrophism symbiosis.But the metabolism growth speed of acidogenic fermentation bacterium is significantly larger than syntrophism fat
The imbalance of fat acid oxidase bacterium and methanogen, this intermediate product metabolism and conversion is easy to cause what anaerobic reactor was acidified
Problem, influences reactor operation stability.The organic acid of accumulation not only seriously suppresses the process of follow-up acetoxylation and methanation,
Can also feedback inhibition residual organic substances hydrolysis acidification, anaerobic metabolic process whole process is obstructed, be unfavorable for treatment of Organic Wastewater imitate
The raising of energy.
Improving high concentrated organic wastewater treatment efficiency and the main thought of operation stability at present is：Build efficient syntrophism fat
Fat acid oxidase bacterium co-cultures body and cultivates acid methanogen.By to pH, the ecology such as ORP (oxidation-reduction potential) and basicity
The research of the factor, finds out and co-cultures the ecological factor scope that body is best suitable for, and is reached by regulating and controlling its most suitable parameter in engineering
The purpose for the treatment of efficiency is improved, but can not still solve the unbalanced problem of intermediate product metabolism and conversion；At present can quilt
The acidproof methanogen being separately cultured out is limited, and these strain growth environment are harsh, to external world the adaptation energy of environment
Power is not strong, and the removal ability to organic matter is limited, and these all can not fundamentally solve the problems, such as that reactor is acidified.
The content of the invention
Problem to be solved by this invention is directed to " reactor is acidified and fluctuation of service " of anaerobic fermentation field appearance
Phenomenon, there is provided a kind of efficient removal volatility organic aliphatic acid, improve the treatment efficiency of high concentrated organic wastewater and stable
The method of property.
In order to solve the problem, the invention provides a kind of syntrophism fatty acid oxidation bacterium and the structure of electricity production bacterium dominant microflora
Method, comprises the following steps：
（1）The enrichment of syntrophism fatty acid oxidation bacterium is by the inoculation sewage treatment plant in continuously stirred tank reactor (CSTR) (CSTR)
Second pond aerobic activated sludge（Solid suspension SS=19.56 g/L）And regulate and control correlation factor and realize.Reactor is by fixing
Hydraulic detention time, with volatility organic aliphatic acid（Propionic acid, butyric acid）It is substrate, the mode for being stepped up influent load starts.
Main clearance by determining substrate, methane production judge whether syntrophism fatty acid oxidation bacterium is enriched with into after stable
Work(.
（2）The enrichment of bacterium is produced electricity then with microbiological fuel cell（MFC）Mode carry out, build MFC reactors inoculation two
Heavy pond aerobic activated sludge, judges to produce electricity the enrichment feelings of bacterium by determining the voltage of electrode two ends generation and the degradation rate of substrate
Condition.
（3）By in the successful electricity production bacterium anode biology film transfer of enrichment to microorganism electrolysis cell (MEC), additional 0.9V is electric
Pressure, according to 1:10 inoculative proportion adds the successful advantage syntrophism fatty acid oxidation bacterium of enrichment to be mixed.MEC reactors
With volatility organic aliphatic acid（Propionic acid or butyric acid）It is substrate, regulation water inlet COD（COD）It is 3000 mg/L, 35
Static experiment is carried out in DEG C insulating box, the clearance of substrate organic volatile acid can reach 96.7%, and hydrogen generation efficiency can be on existing basis
It is upper to improve 1 times.
The main characteristic of the invention lies in that：
1st, the enrichment of syntrophism fatty acid oxidation bacterium：With volatility organic aliphatic acid（Propionic acid or butyric acid）It is substrate, with fixation
The mode that hydraulic detention time is stepped up influent concentration realizes the startup and stable operation of CSTR reactors, with timely height
The characteristics of imitating, start rapid.
2nd, the enrichment of bacterium is produced electricity：Using microbiological fuel cell（MFC）Mode realize, by determine electrode two ends produce
Voltage and the degradation rate of substrate judge to produce electricity the enrichment condition of bacterium, the method is convenient and swift；
3rd, using the end metabolite acetic acid of syntrophism fatty acid oxidation bacterium as electricity production bacterium substrate source, organic matter is realized
Cascade utilization, with it is cost-effective and keep anaerobic system stabilization the characteristics of.
4th, the regulation and control of MEC systems coupling parameter can simultaneously meet the growth conditions of syntrophism fatty acid oxidation bacterium and electricity production bacterium.
The mesostate volatility organic aliphatic acid that the present invention is easily accumulated with anaerobic degradation（Propionic acid or butyric acid）It is bottom
Thing, by coupling syntrophism fatty acid oxidation bacterium and electricity production bacterium, electricity production bacterium can pull syntrophism aliphatic acid oxygen while using acetic acid
Change the metabolism of bacterium, realize the efficient removal of volatility organic aliphatic acid, have to alleviate reactor acidifying problem and improving high concentration
The treatment efficiency and operation stability of machine waste water provide new thinking.With simple structure, operate flexible, stable, nothing
Basicity, the low advantage of operating cost need to be adjusted.
Specific embodiment
The present invention is elaborated according to specific embodiment below.Experimental technique used in following embodiments is such as
Without specified otherwise, conventional method is.Material used, reagent etc. in following embodiments, unless otherwise specified, can be from business
Approach is obtained.
1st, the enrichment of bacterium is produced electricity
The enrichment of electricity production bacterium is by the way of MFC：
Anode material：Carbon cloth（Length × a width of 4 × 2cm）
The preparation of catalytic cathode：The two sides of negative electrode stainless (steel) wire as diffusion layer and Catalytic Layer, in diffusion layer, by PTFE
（Polytetrafluoroethylene (PTFE), wt60%）It is spread evenly across on stainless (steel) wire, is placed in the min of calcination 10 in 350 DEG C of Muffle furnace, repeats above-mentioned
Step 3 time；
In Catalytic Layer, 16mg nano nickel particles are dissolved in the deionized water of 13.28uL, add 53.28uL isopropanols and
The PTFE of 106.7uL（wt60%）In solution, finally be spread evenly across the nano nickel particles liquid after dissolving not by whirlpool concussion 30s
The catalysis of rust steel mesh is laminated, is placed in 350 DEG C of calcination 0.5h in Muffle furnace.
The preparation of MFC water inlets：The phosphate buffer of pH=6.80 is prepared, COD=1600 is adjusted by substrate of glucose
Mg/L, while adding the nutrient and trace element solution of 1ml/L.
Trace element：H3BO350 mg/L；CuCl230 mg/L；MnSO4·H2O 50 mg/L； AlCl350 mg/L；
CoCl2·6H2O 50 mg/L；NiCl250 mg/L, ZnCl2 50mg/L.
Nutrient：NH4Cl 238.8 g/L; KH2PO4 55 g/L; CaCl2 30 g/L; MgSO4 20 g/L and
FeSO4·7H2O 10 g/L.
In the way of static culture after continuous 7 cycles, the voltage of anode biomembrane reaches 450 more than mv and keeps steady
Fixed, the clearance of COD is also stabilized in 90% or so, illustrates that anode biomembrane is enriched with successfully.
2. the enrichment of syntrophism fatty acid oxidation bacterium
Being enriched in CSTR reactors for syntrophism fatty acid oxidation bacterium is completed, first that the second pond of sewage treatment plant is aerobic
Sludge is added in CSTR reactors, makes seed sludge concentration in reactor be 12gVSS/L, the organic wastewater that then will be prepared
Reactor is imported by peristaltic pump, the total effective volume of reactor is 9.6L, and temperature is maintained at 35 ± 1 DEG C by temperature controller.
Reactor head center sets gas collection mouthful, and the gas of generation reaches wet gas flow meter by rubber tube by water seal.
The daily set time determines the pH of water sample, COD, basicity, the concentration of substrate propionic acid and gas componant and ratio in reactor.It is fixed
Hydraulic detention time 48h is constant, is stepped up inlet COD concentration, and initial stage influent COD is 1600mg/L, by bimestrial fortune
OK, when the concentration of water inlet propionic acid is 7000 mg/L, 97% or so, the concentration of metabolite acetic acid exists the clearance stabilization of COD
Below 25mg/L, the ratio of methane reaches 65% or so in gas, illustrates syntrophism fatty acid oxidation bacterium advantage life in the reactor
It is long.
Later stage extracts the DNA of sludge, and syntrophism propionic acid degradation bacterium is detected after sequencingUncultured Smithella spp. Clone(EU888819.1, NCBI database), demonstrates the dominant bacteria that syntrophism fatty acid oxidation bacterium is the anaerobic system
Group.
3. the coupling of syntrophism fatty acid oxidation bacterium and electricity production bacterium
Successful anode biomembrane will be enriched with to be transferred in single chamber MEC, while adding advantage syntrophism fatty acid oxidation strain mud
8.5 mL, anaerobic environment is constructed by way of nitrogen stripping.MEC is the single cell structure of inside diameter 5cm, effective liquor capacity
It is 85mL.Temperature control is at 35 ± 1 DEG C.Additional extra voltage is 0.9V.
The preparation of MEC water inlets：The phosphate buffer of pH=7.00 is prepared, is substrate regulation COD=3000 mg/ with propionic acid
L, while adding the nutrient and trace element solution of 1ml/L, nutrient and trace element solution formula are rich with electricity production bacterium
Collection is consistent.
4. hydrogen-producing acetogens with electricity production bacterium dominant microflora ecological characteristic：
By the continuous monitoring in several cycles, after MEC stable operations, the clearance of propionic acid COD can reach more than 95%.This
When solution ORP scopes be -350 mV of mV ~ -420, the percentage of hydrogen reaches 20% or so.
Claims (6)
1. a kind of syntrophism fatty acid oxidation bacterium and electricity production bacterium dominant microflora construction method, it is characterised in that comprise the following steps：
(1), the enrichment of syntrophism fatty acid oxidation bacterium by continuously stirred tank reactor (CSTR) be inoculated with secondary sedimentation tank of sewage treatment work it is good
Oxygen activity sludge, and regulate and control correlation factor and realize, reactor passes through fixed hydraulic detention time, with volatility organic aliphatic acid
It is substrate, the mode for being stepped up influent load starts；
(2), the enrichment of electricity production bacterium is then carried out in the way of microbiological fuel cell, builds microorganism electrolysis cell inoculation second pond good
Oxygen activity sludge；
(3), by the successful electricity production bacterium anode biology film transfer of enrichment to microorganism electrolysis cell, enrichment is inoculated with according to a certain volume
Successful syntrophism fatty acid oxidation bacterium is mixed.
2. syntrophism fatty acid oxidation bacterium as described in claim 1 and electricity production bacterium dominant microflora construction method, it is characterized in that
The enrichment of syntrophism fatty acid oxidation bacterium is judged by determining the sour clearance of organic volatile, methane production.
3. as described in the appended claim 1 syntrophism fatty acid oxidation bacterium and electricity production bacterium dominant microflora construction method, it is characterized in that produce
The enrichment of electric bacterium is judged by determining the voltage of electrode two ends generation and the degradation rate of substrate.
4. syntrophism fatty acid oxidation bacterium as described in claim 1 and electricity production bacterium dominant microflora construction method, it is characterized in that
After microorganism electrolysis cell adds electricity production bacterium anode biomembrane, additional 0.9V voltages.
5. syntrophism fatty acid oxidation bacterium as described in claim 1 and electricity production bacterium dominant microflora construction method, it is characterized in that
Volatility organic aliphatic acid, especially refers to propionic acid or butyric acid.
6. syntrophism fatty acid oxidation bacterium as described in claim 1 and electricity production bacterium dominant microflora construction method, it is characterized in that
The inoculation volume ratio of fatty acid oxidation bacterium and electricity production bacterium is 1:10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510006685.5A CN104591402B (en) | 2015-01-08 | 2015-01-08 | A kind of syntrophism fatty acid oxidation bacterium and the construction method for producing electricity bacterium dominant microflora |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510006685.5A CN104591402B (en) | 2015-01-08 | 2015-01-08 | A kind of syntrophism fatty acid oxidation bacterium and the construction method for producing electricity bacterium dominant microflora |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104591402A CN104591402A (en) | 2015-05-06 |
| CN104591402B true CN104591402B (en) | 2017-06-16 |
Family
ID=53117519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510006685.5A Active CN104591402B (en) | 2015-01-08 | 2015-01-08 | A kind of syntrophism fatty acid oxidation bacterium and the construction method for producing electricity bacterium dominant microflora |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104591402B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105803001B (en) * | 2016-05-06 | 2019-05-28 | 河海大学 | A method of realizing that excess sludge produces hydrogen using microorganism electrolysis cell |
| CN106011176B (en) * | 2016-07-01 | 2019-08-06 | 河海大学 | Anaerobic fermentation couples the method for realizing that excess sludge produces hydrogen with microorganism electrolysis cell |
| CN111304123B (en) * | 2020-02-28 | 2021-11-16 | 五邑大学 | Compound microbial inoculum for enhancing anaerobic degradation of butyric acid and construction method thereof |
| CN111304124B (en) * | 2020-02-28 | 2021-11-16 | 五邑大学 | Compound microbial inoculum for strengthening propionic acid anaerobic degradation and construction method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102104164B (en) * | 2011-01-19 | 2013-01-02 | 同济大学 | Method for enhancing electrogenesis effect of microbial fuel cell taking sludge as fuel |
| CN103207230A (en) * | 2013-04-02 | 2013-07-17 | 北京林业大学 | Method for constructing dual-chamber microbial fuel cell-type BOD (biochemical oxygen demand) sensor by using potassium permanganate as cathode electron acceptor |
| CN103825041A (en) * | 2014-03-06 | 2014-05-28 | 安徽工程大学 | Single-chamber and film-free anaerobic type microbial fuel cell for processing acidic mine wastewater |
-
2015
- 2015-01-08 CN CN201510006685.5A patent/CN104591402B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104591402A (en) | 2015-05-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Qin et al. | Impact of substrate concentration on anammox-UBF reactors start-up | |
| Sivagurunathan et al. | Overcoming propionic acid inhibition of hydrogen fermentation by temperature shift strategy | |
| Lens et al. | The biological sulfur cycle: novel opportunities for environmental biotechnology | |
| Shoda et al. | Heterotrophic nitrification and aerobic denitrification of high-strength ammonium in anaerobically digested sludge by Alcaligenes faecalis strain No. 4 | |
| Vossoughi et al. | Performance of anaerobic baffled reactor treating synthetic wastewater influenced by decreasing COD/SO4 ratios | |
| Manconi et al. | Combined removal of sulfur compounds and nitrate by autotrophic denitrification in bioaugmented activated sludge system | |
| CN107043160B (en) | Two-phase anaerobic treatment device and process based on magnetite and activated carbon reinforcement | |
| Ahring et al. | Kinetics of butyrate, acetate, and hydrogen metabolism in a thermophilic, anaerobic, butyrate-degrading triculture | |
| CN104591402B (en) | A kind of syntrophism fatty acid oxidation bacterium and the construction method for producing electricity bacterium dominant microflora | |
| CN101565258B (en) | Compound technology of biomembrane and granular sludge for treating sulfur-bearing organic wastewater | |
| CN104478178A (en) | Microbial electrolysis two-section type sludge anaerobic digestion device and method for producing methane by using microbial electrolysis two-section type sludge anaerobic digestion device | |
| CN105176614B (en) | A kind of method of microorganism electrochemical in-situ biogas desulfurization | |
| CN104261559B (en) | A kind of method utilizing microorganism electrolysis strengthening flow lifting type sleeve anaerobic digestion methane-producing reactor to dispose waste liquid | |
| CN107446961A (en) | A kind of method that sulfate reducing bacteria converts for mediation reinforcement sludge carbon source | |
| CN105543282A (en) | A method of increasing an anaerobic biological hydrogen production yield from organic waste water or waste | |
| CN106244661B (en) | Method for producing propionic acid by mixed bacteria system fermentation | |
| CN102249424A (en) | Proton exchange membrane-free microbiological fuel cell sewage treatment system and application method thereof | |
| Pokorna et al. | Adaptation of anaerobic culture to bioconversion of carbon dioxide with hydrogen to biomethane | |
| CN103663680B (en) | Method for cultivating aerobic half-nitrosation granular sludge in continuous flow manner | |
| CN107555591A (en) | A kind of diphasic anaerobic processing unit and technique that direct inter-species electron transmission is built based on ethanol-type fermentation | |
| Vijayaraghavan et al. | Anaerobic digestion and in situ electrohydrolysis of dairy bio-sludge | |
| CN111087071B (en) | Microbial electrochemical device and method for efficiently generating and recovering nitrous oxide from nitrogen-containing wastewater | |
| CN107540085A (en) | It is a kind of to promote the anaerobism method that denitrification methane phase is efficiently carried out simultaneously | |
| CN105800781B (en) | A kind of method of graphite felt materials filling anaerobic reactor processing cassava alcohol waste water | |
| CN104293728A (en) | Method for constructing hydrogen-producing acetogen and sulphate reducing bacterium dominant bacterial communities |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |