CN104585514B - Feed addictive, feed and pork quality modification method - Google Patents
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- CN104585514B CN104585514B CN201510074804.0A CN201510074804A CN104585514B CN 104585514 B CN104585514 B CN 104585514B CN 201510074804 A CN201510074804 A CN 201510074804A CN 104585514 B CN104585514 B CN 104585514B
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Landscapes
- Fodder In General (AREA)
Abstract
The invention belongs to field of fodder, and in particular to feed addictive, the feed comprising the feed addictive, and the method using feed improvement pork quality.The feed addictive of the present invention includes the bacillus metabolism peptide based on the weight % of 75 weight % of feed addictive gross weight 90, more than 0 100mg/kg Organic Selenium, more than 0 40mg/kg Organic Chromium, more than 0% 0.7 weight % organic copper, more than 0% 2.0 weight % Organic Iron, more than 0% 1.0 weight % Organic Manganese, the weight % of 0.5 weight % 1.5 organic zinc, and optional vitamin E and/or vitamin C.Advantage of the invention is that improving the meat of pork, to provide succulence, mouthfeel is fresh and crisp, the healthy pork rich in selenium and zinc.
Description
Technical field
The invention belongs to field of fodder, and in particular to feed addictive, the feed comprising the feed addictive, and use
The method that the feed improves pork quality.
Background technology
Since half a century, pig industry all over the world achieves fast development, and live pig yield and pork yield are significantly
Improve.But while quantity increase, also decline along with meat quality, clenbuterol hydrochloride addition, water-injected meat, Pork form diseased or dead pigs etc. it is tight
The priority of weight problem occurs, and health, natural environment to people etc. bring harm directly or indirectly.Meat quality problem has been drawn
Play the extensive concern of the producer, consumer and nutritionists：Appeal to eat " thin pork " from people's the 1980s, by 90 years
Appeal to eat " quality-assured meat " for people, since 21 century, just " safe meat ", " choice meat " are eaten in more an urgent demand to people.It is this
The change of consumption idea and awareness of safety, reflect that people exist to the degree of concern and demand of live pig meat to a certain extent
Improve constantly.
China is pig big country, as the improvement of people's living standards, meat quality is increasingly by consumers in general's
Pay attention to.Consumer likes lean meat percentage high, the low pork of metabolism of lipid and cholesterol content, while requires that its color and luster is good, meat is tender, more again
Juice, delicious, unsaturated fatty acid content is appropriate.In addition, from the point of view of international vision, improve the self-produced pork pig meat in China to improving this
Native enterprise competitiveness is also benefited.
It is to promote antioxidant ability of organism to improve one of meat approach, mainly including the use of organic trace element and vitamin
E and/or vitamin C.
Prior art proposes a variety of feed addictives, feed or pork quality modification method.
CN102919538A is related to " a kind of feed addictive and batch for improving cad pig growth performance ", the patent application
The feed addictive being related to contains the component of following parts by weight：Dried orange peel 23-25；Hawthorn 23-26；Malt 27-35；Rheum officinale 4-8；Mountain
Medicine 15-23；Vb12 0.001-0.002；Chelated iron 9-11；Sodium butyrate 80-120.It the patent application disclose use and include activity
The fermented bean dregs of small peptide, and point out that fermented bean dregs are easy to intestinal absorption utilization.The patent application mainly passes through the use of Chinese herbal medicine
To realize qi-regulating, tonifying spleen, nourishing the stomach, repair cad pig small intestine chorion, reduce pH value in enteron aisle and suppress harmful bacteria breeding and other effects.
The patent application is confined to improve the growth of cad pig, is not related to the fattening of normal pork pig.
CN103005217A is related to a kind of " feed addictive and batch that improve quality of boar semen ", the patent Shen
Please disclosed feed addictive contain the components of following parts by weight：1 ~ 2 part of broken wall haematococcus pluvialis powder, small peptide albumen 2.5 ~ 5
Part, 2 ~ 4 parts of organic zinc.The patent application is confined to improve the semen quality of herd boar.
CN103461687A is related to a kind of " preparation method of fermented Chinese herbal medicine lactagogue fodder for sows in lactation period ", belongs to microorganism
Fermentation technical field.The feed of the patent application publication is rich in medium-height grass pharmaceutically active substance, active small peptide, lactic acid and probiotics, purport
Stimulating milking sow lactation.
CN103783316A is related to " a kind of lactation period sow health-care feed ", wherein disclosed feed include basal diet and
Nutritional ingredient, wherein basal diet are corn 60-70%, dregs of beans 20-30%, soya-bean oil 1-3%, wheat bran 2-6%, premix 4-7%；Battalion
Form and be divided into：Additional 0.4-0.7% animal derived small peptide, 2-3% plant-derived small peptide, 1-2% on the basis of basal diet
Intestine membrane protein and 10-13ppm isoflavones.The patent application aims to provide health-care feed, to improve the lactation of sow
Amount and content of milk protein, and then the litter weight of weaned piglet can be improved.
CN103478495A is related to " a kind of feed addictive, premix and batch for improving Luchuan Meat ", wherein
Disclosed plant feed addictive contains：Purple perilla seed powder 15-35 parts, vitamin E 5-15 parts, hawthorn 5-25 parts, jujube 10-30
Part, Bio 0.01-0.05 parts, active small peptide class biological agent 0.02-0.06 parts, yeast selenium 0.02-0.08 parts.The addition
Agent is made an addition to 350-900mg/Kg addition in the basal diet of Luchuan pig, improves meat.The patent application publication
Feed addictive is biased toward using plant material, it is intended to improves pork quality by Chinese medicine principle.
CN102783582A is related to " sea-tangle, fish meal, the preparation method of brown sugar and red bean fermented type agent of feed for nursing sow ", its
During use production protease bacillus subtilis, saccharomyces cerevisiae and lactobacillus-fermented.Obtained feed contains active small peptide, breast
Acid and probiotics, it is intended to stimulate milking sow lactation.
CN103892136A discloses a kind of fermented feed, using yeast seeds liquid, lactic acid bacteria culturers liquid and microorganism egg
White enzyme, in confined conditions after fermentation enzymolysis, dry and anti-prevention of sow constipation fermented feed is made.
However, above patent application is not related to be used for pork pig fattening, the feed addictive and phase of pork pig meat are improved
The feed answered.This area lacks the technical scheme for solving above-mentioned technical problem.
The content of the invention
The application is proposed in order to solve one or more technical problems, applicant present in prior art, to provide meat
Pig is fattened, and improves the feed addictive of pork pig meat and corresponding feed.The application further relates to feed pork pig and improves pork
The method of meat.
The theory that present invention applicant advocates is " healthy pig, meat are just fond of eating ", therefore applicant is directed to cultivating health
Pork pig and nice pork is provided.
Present invention applicant had been surprisingly found that, bacillus is metabolized into peptide and Organic Selenium, Organic Chromium, organic copper, Organic Iron, had
Machine manganese, organic zinc and optional vitamin E and/or vitamin C are combined, and obtain improving the feed addition of meat quality
Agent.
Organic Selenium：The principal biological function of selenium is showed by GSH-Px.GSH-Px is by some sub- single
Position (molecular weight 2200) is formed, and each subunit averagely contain selenium atom (Rotruck and Hoekstra,
1973).Therefore, the presence of selenium is directly connected to GSH-Px activity.GSH-Px can be catalyzed reduced glutathione and be changed into oxygen
The reaction of change type glutathione, virose peroxide in body is reduced to alcohol and water, with protect the structure of cell membrane and
Function is not by the infringement and interference of peroxide.GSH-Px this antioxidation, elimination is widely present in body
The illeffects of free radical is particularly significant.
Content of the Organic Selenium in feed addictive is in 0-100mg/kg, preferably 10-90mg/kg, more preferably 20-
80mg/kg, especially preferably 30-70mg/kg, more preferably 40-60mg/kg, most preferably 50mg/kg.Organic Selenium exists
Content in feed addictive can be 0mg/kg, 5mg/kg, 10mg/kg, 15mg/kg, 20mg/kg, 25mg/kg, 30mg/
kg、35mg/kg、40mg/kg、45mg/kg、50mg/kg、55mg/kg、60mg/kg、65mg/kg、70mg/kg、75mg/kg、
80mg/kg, 85mg/kg, 90mg/kg, 95mg/kg or 100mg/kg, above numerical value include ± 2mg/kg reasonable adjusting width
Degree, i.e. 40mg/kg, which is equal to, discloses 38mg/kg, 40mg/kg and 42mg/kg.
Organic zinc：One important function of zinc is antioxidation, and it can be participated in Antioxidative Defense System, passed through
Influence free radical and change the state of anti-oxidant enzyme-to-substrate and influence the antioxidative functional of body.Studies have shown that zinc can disappear
Except internal foreign matter, peroxide radical is reduced, protects cells from caused super oxyradical in oxidative metabolism reaction
Damage to superoxide dismutase activity.
Content of the organic zinc in feed addictive is about in 0.5-1.5%, preferably 1% or so.Organic zinc adds in feed
Content in agent can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4% or 1.5%, the above
Numerical value includes ± 0.05% reasonable adjusting amplitude, i.e., 1.1% is equal to and discloses 1.05%, 1.10% and 1.15%.
Organic Chromium：Addition Organic Chromium can pole significantly improve the drip loss of pork, chromium propionate is added in swine rationses to be carried
The production performance of high animal, strengthen immunity, improve reproductive performance and carcass quality after pig is butchered etc. can be improved.
Content of the Organic Chromium in feed addictive about in 0-40mg/kg, preferably 10-30mg/kg, is more preferably
20mg/kg.Content of the Organic Chromium in feed addictive can be 0mg/kg, 5mg/kg, 10mg/kg, 15mg/kg, 20mg/
Kg, 25mg/kg, 30mg/kg, 35mg/kg or 40mg/kg, above numerical value include ± 2mg/kg reasonable adjusting amplitude, i.e.,
40mg/kg, which is equal to, discloses 38mg/kg, 40mg/kg and 42mg/kg.
Organic copper：Copper is one of essential trace element of animal body, and it can participate in body as the constituent of enzyme
Metabolism；The synthesis of ferroheme and the maturation of red blood cell can also be adjusted；The complete and connective tissue of elastin laminin can also be kept
Maturation.In addition, copper can be with the synthesis of modulating melanin and insulin, the secretion of adrenotrophin.Braude（1945）
It is first
Secondary discovery, 50mg/kg copper is added in Diet For Finishing Pig, the growth of pig can be promoted.
Content of the organic copper in feed addictive is by weight about in 0%-0.7%, preferably 0.5% or so.Organic copper exists
Content in feed addictive can be 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, above numerical value including ± 0.05% it is reasonable
The amplitude of accommodation.
Organic Manganese：Manganese is one of essential trace element of animal body, and it can be as the constituent and enzyme of a variety of enzymes
Activator, participate in carbohydrate, fat and protein metabolism.Numerous studies show, the production performance of manganese and animal, breed work(
Energy, bone formation, carcass quality, eggshell quality and cerebral metabolism etc. are closely bound up.
Content of the Organic Manganese in feed addictive is by weight about in 0%-1.0%, preferably 0.7% or so.Organic Manganese exists
Content in feed addictive can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1.0%, and above numerical value includes ± 0.05%
Reasonable adjusting amplitude, i.e., 0.8% is equal to and discloses 0.75%, 0.80% and 0.85%.
Organic Iron：Iron is the biogenic element of animal body, participates in the material in vital movement in a variety of forms
Metabolism and energetic supersession.Iron is widely distributed in vivo, is present in the form of hemoglobin in red blood cell, in the form of myoglobins
It is present in muscle, is present in the form of transferrin and ferritin in serum, is present in placenta in the form of the ferritin of uterus
In, it is present in the form of lactoferrin in breast, is present in the form of ferritin and flavine ferritin in liver and spleen；Iron or cell
Chromo-oxidase, peroxidase, catalase, the important component of xanthine oxidase.Iron status and animal body
Grow closely related with health.
Content of the Organic Iron in feed addictive is by weight about in 0%-2.0%, preferably 1% or so.Organic Iron is being raised
Content in feed additives can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4% or
1.5%, above numerical value includes ± 0.05% reasonable adjusting amplitude, i.e., 1.1% is equal to and discloses 1.05%, 1.10% and
1.15%。
What vitamin E and/or vitamin C were optionally present in feed addictive.If vitamin E be present, its content
For 150-250mg/kg, preferably from about 200mg/kg or so.If vitamin C be present, its content is 10-50mg/kg, preferably from about
30mg/kg.Certainly on the premise of not influenceing the Expected Results of feed addictive and not having a negative impact to feeding object,
Other appropriate contents can also be used, it is not limited to concrete content example provided herein.
Bacillus is metabolized peptide：Or it can be described as bacillus culture and metabolite, also referred to as fermentation of bacillus
Thing.Containing a large amount of small peptides in the fermentation of bacillus thing of protein raw materials, small peptide can improve the pork pig speed of growth, reduce feed-weight ratio,
Energy substitute antibiotics and antimicrobial, feed the feed containing small peptide product and the growth-promoting effect and economic benefit of pork pig are better than not
Use the feed of small peptide product；Small peptide can improve the digestibility of feed protein；It is reported that it is big that 0.5% is added in swine rationses
It legumin hydrolysate, can substantially increase lean meat percentage, hog on hook fat content be reduced, so as to improve meat quality.
4 kinds of neutral, alkalescence, acidic amino acid and imino acid active transport systems in animal body be present in free amino acid
System, inverse concentration gradient transhipment is carried out by Na+ pumps or non-Na+ pumps delivery system.Different from free amino acid, small peptide is dynamic
There may be following 3 kinds of transporting mechanisms in object：（1）H+/Na+ with pH dependences exchanges delivery system, does not consume three phosphorus
Adenosine monophosphate（ATP）.Think through research, electrochemical gradient of the power from proton of small peptide transhipment, proton is to intracellular transport
Power drives small peptide to intracellular motility, and small peptide causes the pH value of cell to decline so as to entering cell in the form of actively spreading,
Na+/H+ passages are activated, and H+ is released cell, and the pH value of cell is able to return to original level.When lacking H+ gradients
When, by film outside waste concentration carry out；When the high outside and low outside H+ concentration of cell be present, then cotransported with the life electricity of inverse substrate
Carry out.（2）The active transport process of H+ or Na2+ " concentration is relied on, it is necessary to consume ATP, but it is totally different from enterocyte pair
The active transport of free amino acid, it is an independent process.This transporting mode is in anoxic or quilt when metabolic poison be present
Suppress.（3）Glutathione（GSH）Movement system.GSH has important anti-oxidation function, thus GSH movement systems in the cell
May possess the physiological significance of uniqueness.But its mechanism is not fully aware of at present.Vincerzini thinks, GSH transmembrane transport
System is relevant with Na+, K+, Li+, Na2+, Mn+ concentration gradient, and unrelated with H+ concentration.
The absorption of small peptide has the advantages that power consumption is low, transport speed is fast, carrier is not easy saturation；And free amino acid absorbs
Slowly, the easy saturation of carrier, power consumption is big during absorption.There is scholar to think, peptide carrier absorption ability could possibly be higher than various amino acid carriers and inhale
Receipts ability it is total.And the small peptide mixer perfusion experiment of duodenum to animals such as pig, chickens shows, the absorptivity of small peptide mixer
Apparently higher than ispol.The reason for amino acid residue is rapidly absorbed in small peptide, except peptide mechanism of absorption in itself in addition to, can
Can be that absorption of the peptide to amino acid or its residue in itself has facilitation.According to Bamba etc., make intestines by substrate of small peptide
Win brush-shaped membrane capsule（BBMV）Aminopeptidase activities and amino acid carrier activity increased with number.Brandsch's grinds
Study carefully result to also indicate that, the fermentation protein hydrolysate being present in jejunum（Junket endorphin）L-Leu can be made to enter the dynamic of enterocyte
Mechanical constant increases.Further, since the presence of peptide carrier reduces competition of the single amino acids in absorption, so as to reduce ammonia
Antagonism between base acid, it is also possible to the reason for small peptide high-selenium corn.
Bacillus metabolism peptide of the present invention is manufactured by following specific process.
1）Protein raw materials, carbon source and water are configured to nutrient solution, the content of protein raw materials such as dregs of beans, potato etc. can be
20-30g/100ml, the content of carbon source such as glucose can be 0.1-1g/100ml；
2）Bacillus is inoculated into nutrient solution, inoculum concentration can be 5-10 weight %；The shaken cultivation 18- at 30-37 DEG C
After 30h, protease is added, the addition of protease can be 300-1000U/g protein raw materials；In 35-50 DEG C and pH6.0-7.5 after
Continuous culture 6-12h, obtains small peptide zymotic fluid；Either：Protease is added into nutrient solution, in 35-50 DEG C and pH6.0-7.5 enzymes
After solving 6-12h, bacillus is inoculated into nutrient solution, continues shaken cultivation 18-30h at 30-37 DEG C, obtains small peptide fermentation
Liquid；
3）Small peptide zymotic fluid is dried, obtains the bacillus metabolism peptide.
Inventor has been surprisingly found that, by by specific fermentation of bacillus thing produced by the present invention and Organic Selenium,
Organic Chromium, organic zinc and vitamin E/C（Optionally）Etc. combining, and pork pig fattening stage is particularly used for, so as to
Succulence is provided, mouthfeel is fresh and crisp, the healthy pork rich in selenium and zinc.
Specifically, following technical scheme is claimed in the present invention.
1. a kind of feed addictive, it includes and is based on feed addictive gross weight：
75 weight %-90 weight % bacillus metabolism peptide,
Organic Selenium more than 0-100mg/kg,
Organic Chromium more than 0-40mg/kg,
More than 0%-0.7 weight % organic copper,
More than 0%-2.0 weight % Organic Iron,
More than 0%-1.0 weight % Organic Manganese,
0.5%-1.5 weight % organic zinc, and
Optional vitamin E and/or vitamin C.
2. the feed addictive described in technical scheme 1, it is characterised in that the bacillus metabolism peptide is protein raw materials
Bacillus liquid tunning.
3. the feed addictive described in any one of preceding solution, it is characterised in that the bacillus metabolism peptide leads to
Cross following methods manufacture：
1）Protein raw materials, carbon source and water are configured to nutrient solution；
2）Bacillus is inoculated into nutrient solution, at 30-37 DEG C after shaken cultivation 18-30h, adds protease, in
35-50 DEG C and pH6.0-7.5 is continued to cultivate 6-12h, obtains small peptide zymotic fluid；Either：Protease is added into nutrient solution, in
After 35-50 DEG C and pH6.0-7.5 enzymolysis 6-12h, bacillus is inoculated into nutrient solution, continues vibration training at 30-37 DEG C
18-30h is supported, obtains small peptide zymotic fluid；
3）Small peptide zymotic fluid is dried, obtains the bacillus metabolism peptide.
4. the feed addictive described in any one of preceding solution, it is characterised in that in the bacillus metabolism peptide
The content of small peptide is 20-40 weight %.
5. the feed addictive described in any one of preceding solution, it is characterised in that the bacillus is metabolized peptide
From protein raw materials, the protein raw materials are selected from：Soybean, dregs of beans, rapeseed meal, cotton dregs, soybean protein isolate, FSPC, horse
Bell potato, rice, wheat and corn and combinations thereof.
6. the feed addictive described in any one of preceding solution, it is characterised in that the bacillus is selected from：Withered grass
Bacillus, bacillus licheniformis, bafillus natto and combinations thereof.
7. the feed addictive described in any one of preceding solution, it is characterised in that the feed addictive includes base
In feed addictive gross weight 150-250mg/kg vitamin E, preferably from about 200mg/kg；With gross weight 10-50mg/kg dimension
Raw plain C, preferably from about 30mg/kg.
8. a kind of feed, its feed addictive described in comprising basal feed and any one of preceding solution, the feeding
The addition of feed additives is 1-5 kilograms of basal feed per ton（1-5 weight ‰）, preferably 1-3 kilograms（1-3 weight ‰）.
9. the feed described in technical scheme 8, it is characterised in that the feed is used for pig or fowl.
10. a kind of method for improveing pork quality, it includes educating with the feed feeding 70-100kg described in technical scheme 8
Big porker 30-50 days.
Compared to the prior art, the advantage of the invention is that：
1）Peptide is metabolized by bacillus to combine with organic zinc, selenium, chromium, iron, copper, manganese, Product Green environmental protection, meets health
Cultivation theory；
2）Less investment output benefit is big, and because feed cost per ton is at 80 yuan or so, every pig is total 20 yuan, using this hair
The pig of bright scheme raising, hair jin oversold 3-5 maos per jin, 60 ~ 100 yuan of output, net profit is at 40 ~ 80 yuan；
3）The pig raised using the present invention program, Tu's postmortem muscle is glossy, in pale red, fat white；Cooked meat
Mouthfeel is very crisp, it is easy to is chewed；Remaining meat slag seldom easily swallows after chewing；Meat soup is delicious, enjoys endless aftertastes；
4）The pork that the pig raised through the present invention program is obtained has the organic trace elements such as the muscle selenium improved and zinc
Content, meet current consumption idea；
5）The present invention is metabolized the organic assembling of peptide and organic trace element by bacillus, to growing-finishing pigs performance,
Selenium and Zn content in meat and pork produce actively impact.
Brief description of the drawings
Fig. 1 shows the test group pigskin table phase closeup photograph of embodiment 2；
Fig. 2 shows the control group pigskin table phase closeup photograph of embodiment 2.
Embodiment
Make specifically deep description, but wherein disclosed each specific side to the present invention below by specific embodiment
Face is used for illustrative purposes only, and any restrictions effect is not produced to protection scope of the present invention.
The preparation of embodiment 1-feed addictive of the present invention
Feed addictive includes 80 weight % bacillus metabolism peptide, and 50mg/kg Organic Selenium, 40mg/kg's is organic
Chromium, 1 weight % organic zinc, the Organic Iron of 1% weight, the Organic Manganese of 0.7% weight, the organic copper of 0.5% weight, 200mg/kg's
The soybean protein of vitamin E and 30mg/kg vitamin C and surplus, the weight % of above components total 100.
Above percentage by weight is based on the gross weight meter of feed addictive.
Wherein, bacillus metabolism peptide manufactures by the following method：
1）The preparation of nutrient solution：50kg crude protein contents are 46% dregs of beans, and 20kg crude protein contents are 50% corn egg
White powder, 15kg crude protein contents are 35% rapeseed meal, and 20kg crude protein contents are 48% peanut meal, and 95kg crude protein contents are 60%
Rice protein powder, 100kg crude protein contents are 65% FSPC, and 4kg sucrose and 4g glucose are arrived with water constant volume
1000L, adjust reacting liquid pH value with hydrochloric acid or sodium hydroxide and sterilized 18 minutes to 6.8,121 DEG C；
2）It is prepared by bacillus metabolism peptide zymotic fluid：Sterilizing terminates, and after culture-liquid temp drops to 45 DEG C, adds in 4.2kg
Property protease（700U/g protein raw materials）, 45 DEG C of enzymolysis 12h, obtain small peptidase hydrolyzed liquor；By the Bacillus by culture
Liquid is accessed in small peptidase hydrolyzed liquor, and it is about 5 × 10 to make bacillus subtilis or Bacillus licheniformis bulk concentration in enzymolysis liquid7cfu/
Ml, in 32 DEG C of constant-temperature tables, 200r/min shaken cultivation 24h, obtain small peptide zymotic fluid；
3）Bacillus metabolism peptide zymotic fluid is placed in 80 DEG C of oven for drying to moisture and is less than 10%, crushes, crosses 60 mesh sieves,
Obtain the bacillus metabolism peptide.
The product type of Organic Selenium is Se-enriched yeast SE2000, is produced by Guangzhou Prosyn Bio-Feed Co., Ltd..
The product type of Organic Chromium is Cr-enriched yeast CR2000, is produced by Guangzhou Prosyn Bio-Feed Co., Ltd..
The product type of organic zinc is that zinc-rich is apt to first zinc treasured 10-HZN, is given birth to by Guangzhou Prosyn Bio-Feed Co., Ltd.
Production.
The product type of Organic Iron is that rich iron is apt to rod iron treasured 10-HFE, is given birth to by Guangzhou Prosyn Bio-Feed Co., Ltd.
Production.
The product type of Organic Manganese is that rich manganese is apt to first manganese treasured 10-HMN, is given birth to by Guangzhou Prosyn Bio-Feed Co., Ltd.
Production.
The product type of organic copper is that copper-rich is apt to first copper treasured 10-HCU, is given birth to by Guangzhou Prosyn Bio-Feed Co., Ltd.
Production.
Above-mentioned feed addictive is well mixed various composition by stirring 10-30 minutes by mixer at normal temperatures
And obtain.
2-market pig of embodiment is fattened
In the present embodiment, to body weight in 80kg or so（±5kg）Ternary pigs fattened, feeding time be 40 days.
The packet and management of market pig
The column of big porker 8 of physical health is selected, per 25, column.8 columns are divided into 2 parts, the column of control group 4, the column of test group 4.
Control group pig control daily ration（The self-produced fattening pannage of Guangxi GL companies）Feeding, test group pig is on pig farm pair
On the basis of daily ration, with embodiment 1 prepare feed addictive substitute control daily ration in etc. weight dregs of beans, obtain test group day
Grain is fed.The addition of feed addictive is about 3 kilograms of daily ration of control per ton.
The composition and trophic level of control daily ration are referring to table 1 below.
Table 1：
Evaluation method
The evaluation of the following aspects is carried out to the market pig after fattening.
1）Fur table is mutually evaluated：Main relatively following three aspects：A. whether skin is bright；B. the length of hair；C. hair is dilute
The degree of dredging；
2）Yellowish pink evaluation：Establishing criteria picture scorecard, blind comment is carried out one by one to each meat sample；
3）Muscle drip loss is evaluated：Do not apply any external force, only by Action of Gravity Field, weighed by drip loss.Tool
Body mode of operation is：Sliced meat are repaiied into epimysium, meat sample is sling with silk thread or metal hook, one plastics weighed in advance of overcoat
Bag, sack are fastened and meat sample are enclosed in bag, the water droplet that sufficient space receives meat sample to ooze out are left in bag, then in refrigerator（0~4
℃）Middle suspension removes polybag after 24 hours, and sucking meat sample surface moisture with filter paper is re-weighed.
4）Mouthfeel is evaluated：One jin of pig neck is taken, is put into the water of ebuillition of heated（2 jin of water）, appropriate salt is put into,
It is cooked, mouthfeel is evaluated by testing crew.
5）The assay of selenium and zinc：Lean pork taken under the spinal column of a hog is taken with rear pig from butchering respectively, rejects connective tissue unnecessary thereon
And fat, physiological saline wash away bloodstain, filter paper blots physiological saline thereon, is cut into thin slice by knife, puts baking oven 65 degree 8 hours,
Muscle after drying is crushed, in weighed on precision balances, with atomic absorption detecting muscle selenium Zn content.
Evaluation result
The data obtained in above evaluation procedure carry out one-way analysis of variance using excel 2003.
1）Fur table is mutually evaluated：It can show that the pigskin of test group is red, capillary from the description and field observation of raiser,
Oligotrichosis；The pig relative test group of control group is turned white, and hair is longer, and hair is relatively more（Respectively referring to Fig. 1 and Fig. 2）.
2）Yellowish pink evaluation：Yellowish pink evaluation result is as shown in table 2 below.
Table 2：
From Table 2, it can be seen that test group scores apparently higher than control group.Also, with the extension of standing time, control
The color change of group meat is bigger, and the change of test group pork colour is not notable.
3）Muscle drip loss is evaluated：The evaluation result is as shown in table 3 below.
Table 3：
Test group is can be seen that from the data of table 3 and control group drips rate difference for 0.28%.In control group group difference compared with
Greatly, peak 2.51%, minimum 0.94%, difference about 1.6%；And test group peak is 1.35%, minimum is
0.82%, difference 0.53%.It can be seen, test group is integrally better than control group.
4）Mouthfeel is evaluated：Commented by the way that scene is blind, testing crew thinks that the mouthfeel of meat sample 2 is more preferable, pork succulence and more crisp, perfume
Gas is denseer, and palatability is also relatively more preferable；And the mouthfeel of meat sample 1 is less crisp, feel there is slag after chewing, and fragrance is inadequate.Finally
No. 5 meat samples that meat sample 2 is derived from test group are disclosed, and meat sample 1 is derived from No. 1 meat sample in control group.
5）Zinc-content determination result is shown in table 4.
Table 4：
From the point of view of the testing result of table 4, control group（41.45mg/kg）Significantly lower than test group（50.61mg/kg）, experiment
Group pig is higher by about 10mg/kg than control group；Analysis of variance, significant difference between control group and test group be present（p<0.05）, say
The addition of bright organic zinc generates beneficial effect to zinc deposition.
Determination of Selenium result is shown in table 5.
Table 5：
From the point of view of the testing result of table 5, control group selenium average content is significantly lower than test group, and test group selenium average content is
1.77 times of control group.Significant difference be present between Analysis of variance control group and test group（p<0.05）, illustrate adding for Organic Selenium
Add and obvious facilitation is produced to selenium deposition.
Interpretation of result
Evaluated more than, inventor thinks that trace element and bacillus metabolin produce notable shadow to meat percentage of water loss
Ring, they strengthen the activity of superoxide dismutase in muscle to remove free radical.And free radical can be destroyed on cell membrane not
Saturated fatty acid structure, the mobility of cell membrane is as a result reduced, cause cell fragility to become big, cell, which is easily broken, causes content
Outflow, drip loss is increased, reduce nutrition and the standing losses of meat.The shadow that meat yellowish pink is reflected by meat surface light
Ring, runny ooze out can make surface light reflection increase in meat, and color and luster of meat brightens, bleached.Contain in intramuscular fat
Unrighted acid needed by human, and the special flavor material of meat is also primarily present in intramuscular fat, intramuscular fat
The tenderness and palatability of meat can also be improved by cutting off the cross-linked structure of fascicula.
The preparation of embodiment 3-control feed additive and market pig fattening
The present embodiment uses and the identical composition of embodiment 1 and step preparation control feed additive, wherein the addition of control 1
Insufficient amount of bacillus is metabolized peptide；Organic Selenium is not added in control 2；Organic Chromium is not added in control 3；Organic zinc is not added in control 4；
Specific feed addictive composition（Vitamin E and C are omitted）It is listed in the table below 6.
Table 6：
According to the market pig fattening method of embodiment 2, above-mentioned five kinds of feed addictives are applied to body weight about 80kg（±
5kg）Ternary pigs fattening, feeding time and embodiment 2 are identical.
Compared with the ternary pigs that the feed addictive of the present invention of embodiment 1 is fattened, the ternary pigs that are obtained by fed control 1
In evaluation method 3）Muscle drip loss is evaluated and evaluation method 4）Mouthfeel evaluation these two aspects produces deterioration；Pass through feeding pair
According to the ternary pigs that 2-4 is obtained in evaluation method 3）The evaluation of muscle drip loss, evaluation method 4）Mouthfeel is evaluated and evaluation method 5）
This three aspect of the assay of selenium and zinc produces deterioration；Therefore the feed addictive of the present invention of embodiment 1 is better than control 1-4.
Further, from embodiment 1-4 on the whole, combined by the specific products of the application, it is possible to achieve comprehensive regulation,
And then improving the effect of meat, this effect is not easy to realize by the way that one or two kind of component therein is used alone.
Embodiment 4-control market pig fattening
The present embodiment is used and the identical method of embodiment 2 is fattened to end article pig, equally using embodiment 1
Feed addictive, difference are that end article pig had both included body weight in 80kg or so（±5kg）Ternary pigs, also including body weight not
For the ternary pigs of sufficient 50kg and body weight more than 100kg as control market pig, this embodiment is intended to the feed addition of the test present invention
Action effect of the agent for the pork pig of different growth phases.
From the pork pigs of different production phases from the point of view of the front and rear body weight growth rate of feeding, feed addictive of the invention for
Body weight is in 80kg or so（±5kg）Pork pig effect it is the most notable.It has been recognised by the inventors that as underweight 50kg, not yet enter
Enter fattening stage, it is limited by improvement of the feed addictive of the present invention for meat, therefore using under the economy of additive
Drop.On the other hand, when body weight is more than 100kg, additive equally declines for the effect that meat improves, and this is probably because body
It is again not far more than the body weight that distance after 100kg is delivered for sale, therefore feeding time is shorter, feeding effect reduces so that additive
Weakened using motivation.
Inventor it is thus regarded that the present invention feed addictive be preferred for growing and fattening pigs of the body weight in 70-100kg, thus change
The meat of kind pork, to provide succulence, mouthfeel is fresh and crisp, the healthy pork rich in selenium and zinc.
In summary discuss, inventors be surprised to learn that the feed addictive of the present invention is added in feed can be obviously improved fertilizer
Pock Color, it is water rate and pork is in good taste；The content of muscle selenium and zinc can be improved simultaneously.As a result to lifting meat quality, wound
Making brand pork has very high application value.
The embodiment that the present invention provides is intended to help skilled in the art to understand and implement technical scheme, and
Restriction effect is not produced to the details of technical scheme.Ins and outs disclosed in different embodiments and/or embodiment can
With combination, equivalent substitution, to obtain new embodiment or embodiment.Specific data that the present invention provides while cover are based on
The fluctuation up and down of the numerical value zone of reasonableness, to cover equivalent substitution and measurement error that may be present.
Claims (3)
1. a kind of feed addictive for improveing meat quality of fattening pigs, it includes and is based on feed addictive gross weight：
80 weight % bacillus metabolism peptide, 50mg/kg Organic Selenium, 40mg/kg Organic Chromium, 1 weight % organic zinc, 1%
The Organic Iron of weight, the Organic Manganese of 0.7% weight, the organic copper of 0.5% weight, 200mg/kg vitamin E and 30mg/kg dimension
Raw plain C and surplus soybean protein, the weight % of above components total 100；
Wherein, bacillus metabolism peptide manufactures by the following method：
1）The preparation of nutrient solution：50kg crude protein contents are 46% dregs of beans, and 20kg crude protein contents are 50% corn protein powder,
15kg crude protein contents are 35% rapeseed meal, and 20kg crude protein contents are 48% peanut meal, and 95kg crude protein contents are 60% rice
Albumen powder, 100kg crude protein contents are 65% FSPC, 4kg sucrose and 4g glucose, with water constant volume to 1000L,
Reacting liquid pH value is adjusted with hydrochloric acid or sodium hydroxide to sterilize 18 minutes to 6.8,121 DEG C；
2）It is prepared by bacillus metabolism peptide zymotic fluid：Sterilizing terminates, and after culture-liquid temp drops to 45 DEG C, adds 4.2kg 700U/
The neutral proteinase of g protein raw materials, 45 DEG C of enzymolysis 12h, obtains small peptidase hydrolyzed liquor；Bacillus liquid by culture is connect
Enter in small peptidase hydrolyzed liquor, it is about 5 × 10 to make bacillus subtilis or Bacillus licheniformis bulk concentration in enzymolysis liquid7Cfu/ml,
In 32 DEG C of constant-temperature tables, 200r/min shaken cultivation 24h, bacillus metabolism peptide zymotic fluid is obtained；
3）Bacillus metabolism peptide zymotic fluid is placed in 80 DEG C of oven for drying to moisture and is less than 10%, is crushed, is crossed 60 mesh sieves, obtain
The bacillus is metabolized peptide.
2. a kind of fattening pannage, its feed addictive described in comprising basal feed and claim 1, the feed addictive
Addition be 1-5 kilograms of basal feed per ton.
3. a kind of method for improveing meat quality of fattening pigs, it is included with the fattening pannage feeding 70-100kg described in claim 2
Growing and fattening pigs 30-50 days.
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| CN105767555A (en) * | 2016-04-08 | 2016-07-20 | 甘肃农业大学 | Feed additive capable of improving quality and flavor of pork |
| CN106490370A (en) * | 2016-12-16 | 2017-03-15 | 万旻 | A kind of biological feedstuff for improving meat quality |
| CN107361200A (en) * | 2017-07-17 | 2017-11-21 | 山东巧宾农业科技有限公司 | Application of the blattaria in terms of animal tallow ratio is reduced |
| CN107873628A (en) * | 2017-10-21 | 2018-04-06 | 赵三存 | A kind of cultural method of zinc-rich pig |
| CN108294183A (en) * | 2018-03-29 | 2018-07-20 | 廊坊瑞康饲料有限公司 | It improves growing and fattening pigs nitrogen use efficiency and improves the feed addictive of meat |
| CN113481110B (en) * | 2021-08-23 | 2023-04-11 | 广西壮族自治区农业科学院 | Preparation method and application of Auricularia polytricha selenium-rich culture |
| CN116158401A (en) * | 2023-02-22 | 2023-05-26 | 江苏省家禽科学研究所 | Diet dilution research method for improving bone and digestive tract development of laying hens before parturition |
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Granted publication date: 20180302 Pledgee: Bank of China Limited Guangzhou Development Zone Branch Pledgor: GUANGZHOU PROSYN BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2022980027153 |
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Denomination of invention: Feed additives, methods for improving the quality of feed and pork meat Granted publication date: 20180302 Pledgee: Bank of China Limited Guangzhou Development Zone Branch Pledgor: GUANGZHOU PROSYN BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2024980009788 |
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