CN104584882A - Shiitake cultivation method - Google Patents
Shiitake cultivation method Download PDFInfo
- Publication number
- CN104584882A CN104584882A CN201510085941.4A CN201510085941A CN104584882A CN 104584882 A CN104584882 A CN 104584882A CN 201510085941 A CN201510085941 A CN 201510085941A CN 104584882 A CN104584882 A CN 104584882A
- Authority
- CN
- China
- Prior art keywords
- mushroom
- shiitake
- tang
- turning
- cultivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a shiitake cultivation method, and relates to a technology applying shiitake tangs to shiitake cultivation. Compared with the prior art, the shiitake cultivation method has the advantages as follows: (1) a shiitake tang soaking material is added to be mixed with cultivation raw materials for stacking fermentation; beneficial microorganism is promoted to proliferate; microbial flora in the materials is improved; the catalysis disassociation of the cultivation materials is accelerated; (2) the cultivation materials after fermentation have high-activity beneficial bacteria and active metabolite, and have promotion effect on the growth of shiitake hypha; the shiitake hypha is strong in growing vigor and high in infectious microbe resisting capability; (3) after the second and third waves of shiitake is harvested, shiitake tang lixivium is good in effect of rejuvenating mycelium; promoting action is played on formation of shiitake primordium and growth of sporocarp; the biotransformation rate of the third and fourth waves of shiitake is high; (4) manual labor and resources of coal and the like are saved; environmental pollution is reduced; the shiitake cultivation method conforms to the low-carbon environment-friendly concept.
Description
Technical field
A kind of method for cultivating mushroom of the present invention, is the technology be applied to by mushroom tang in cultivating champignon, belongs to fungus growing technique field.
Background technology
Mushroom is second-biggest-in-the-world edible mushroom.Due to its delicious flavour, fragrance oozes people, nutritious, not only ranks on straw mushroom, flat mushroom, white mushroom, and have the reputation of " fungi queen ".
The traditional cultivation method of mushroom is cured material bag-cultured, and planting material carries out inoculated and cultured after high pressure or normal-pressure sterilization, and the contour molecular organic material of the cellulose now in material is degraded, miscellaneous bacteria in material is also killed, therefore, when planting material sends out bacterium, mycelial growth is fast, and living contaminants is few.But along with the significantly rise of the price of labour power and fuel price, grog cultivation cost increases severely, and high-temperature sterilization also causes the loss of planting material nutriment, causes planting material conversion ratio to reduce simultaneously.
Mushroom is famous and precious edible one of the medicinal fungus of holding concurrently in the world, and current China mushroom production accounts for 70% of world's mushroom gross yield, has the equal mushroom culture in the province of more than 70% in China.Because mushroom stems stiffness of foot in children, content of cellulose are higher, palatability is poor, and therefore, mushroom is being eaten raw or in process, the tang of mushroom can remove as offcuts by the producer, causes the mushroom tang accounting for mushroom weight about 30% to become discarded object.
Summary of the invention
For the deficiencies in the prior art, the present invention adds the immersion of mushroom tang and expects to mix and stir to carry out banking up with culturing raw material to ferment, and do not add insecticide, bactericide during windrow, the planting material after fermentation is directly taped against ridge-up bed cultivation, and the mushroom quality of producing is good, output is high.
The technical solution adopted in the present invention is:
A kind of method for cultivating mushroom, comprises batching, ferments, makes the bed, sows, sends out bacterium, earthing, annesl, management of producing mushroom, concrete operation step:
(1) prepare burden: cotton seed hulls 72-76%, soya-bean cake 16-18%, mushroom tang 4-5%, lime 3-4%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%; Described mushroom tang is the offcuts in Lentnus edodes process, is ground into the particle of 2mm-3mm after drying;
(2) ferment, make the bed, sow, send out bacterium, earthing: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leachate is admixed in cotton seed hulls, soya-bean cake compound together with mushroom stems slag uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation, after material temperature reaches 60 DEG C, keep 24h, then carry out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%, the planting material fermented, after heat radiation, is directly taped against ridge-up bed sowing and sends out a bacterium, when mycelia sends out material full, cover the sandy loam that 1cm is thick, moisturizing annesl enters management of producing mushroom;
(3) management of producing mushroom: after the second damp mushroom, the 3rd damp mushroom are gathered, sprays the mushroom tang leachate of 0.01-0.02g/ml comprehensively in mushroom bed, every square metre is sprayed 5-7kg, with rejuvenation mycelia; The preparation method of described leachate is: mushroom tang particle is added clear water by the weight ratio of 20 times, soak 18h-24h at normal temperatures, filter and remove residue, then complement to the original water yield, obtain the mushroom tang leachate that concentration is 0.05g/ml, add clear water dilution and use.
Mushroom tang is applied to the method for cultivating champignon by the present invention, and apart from outside explanation, other operating procedure is all undertaken by this area routine techniques.
The present invention's advantage is compared with prior art:
1. mushroom tang leachate is full-natural nutritive liquid, containing glucide, amino acid, peptide class, nucleic acid and trace element, bio-hormone also containing abundant species, also the bioactivator do not leached in a large number is had in mushroom stems slag, add and expect to mix and stir to carry out banking up with culturing raw material to ferment with the immersion of mushroom tang, promote beneficial microbe breeding, improve the microorganism species in material, accelerate the catalytic decomposition of planting material;
2. the planting material after fermentation has high activity beneficial bacterium and active metabolite thereof, has Promotive effect, show as prosperous, the anti-miscellaneous bacteria ability of mycelium growth vigor strong to the growth of mushroom mycelium;
3., after the second damp mushroom, the 3rd damp mushroom are gathered, effective with mushroom tang leachate rejuvenation mycelia, have facilitation to the formation of the former base of mushroom and the growth of fruit body, the biological transformation ratio of third and fourth damp mushroom is high;
4. save the resource such as manpower, coal, decrease environmental pollution, meet low-carbon environment-friendly theory.
Embodiment
Below in conjunction with embodiment, the invention will be further described, and what do not illustrate in detail in embodiment is all state of the art.
Embodiment 1: a kind of method for cultivating mushroom, comprises batching, ferments, makes the bed, sows, sends out bacterium, earthing, annesl, management of producing mushroom, concrete operation step:
1. prepare burden: cotton seed hulls 74%, soya-bean cake 17%, mushroom tang 4.5%, lime 3.5%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leachate is admixed in cotton seed hulls through prewetting, soya-bean cake compound together with mushroom stems slag uniformly, stockpile is become high 1.2m-1.5m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. make the bed, sow, send out bacterium, earthing: the planting material fermented is after heat radiation, and be directly taped against ridge-up bed sowing and send out a bacterium, when mycelia sends out material full, cover the sandy loam that 1cm is thick, moisturizing annesl enters management of producing mushroom;
5. management of producing mushroom: after having adopted the second damp mushroom, sprays the mushroom tang leachate of 0.01g/ml in mushroom bed, every square metre is sprayed 5kg; After having adopted the 3rd damp mushroom, spray the mushroom tang leachate of 0.02g/ml, every square metre is sprayed 7kg, and by mushroom tang leachate rejuvenation mycelia, fruiting is effective, and the biological efficiency of third and fourth damp mushroom reaches 74.8%, and total biological efficiency reaches 158.6%.
Embodiment 2: a kind of method for cultivating mushroom, comprises batching, ferments, makes the bed, sows, sends out bacterium, earthing, annesl, management of producing mushroom, concrete operation step:
1. prepare burden: cotton seed hulls 74%, soya-bean cake 17%, mushroom tang 4.5%, lime 3.5%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leachate is admixed in cotton seed hulls through prewetting, soya-bean cake compound together with mushroom stems slag uniformly, stockpile is become high 1.2m-1.5m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. make the bed, sow, send out bacterium, earthing: the planting material fermented is after heat radiation, and be directly taped against ridge-up bed sowing and send out a bacterium, when mycelia sends out material full, cover the sandy loam that 1cm is thick, moisturizing annesl enters management of producing mushroom;
5. management of producing mushroom: after having adopted the second damp mushroom, the clear water of every square metre of sprinkling 5kg; After having adopted the 3rd damp mushroom, the clear water of every square metre of sprinkling 7kg, the biological efficiency of third and fourth damp mushroom has been 54.4%, and total biological efficiency is 138.2%.
Embodiment 3: a kind of method for cultivating mushroom, comprises batching, ferments, makes the bed, sows, sends out bacterium, earthing, annesl, management of producing mushroom, concrete operation step:
1. prepare burden: cotton seed hulls 74%, soya-bean cake 17%, mushroom tang 4.5%, lime 3.5%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then the leachate after removing slag is admixed uniformly in the cotton seed hulls through prewetting, soya-bean cake compound, stockpile is become high 1.2m-1.5m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. make the bed, sow, send out bacterium, earthing: the planting material fermented is after heat radiation, and be directly taped against ridge-up bed sowing and send out a bacterium, when mycelia sends out material full, cover the sandy loam that 1cm is thick, moisturizing annesl enters management of producing mushroom;
5. management of producing mushroom: after having adopted the second damp mushroom, sprays the mushroom tang leachate of 0.01g/ml in mushroom bed, every square metre is sprayed 5kg; After having adopted the 3rd damp mushroom, spray the mushroom tang leachate of 0.02g/ml, every square metre is sprayed 7kg, and by mushroom tang leachate rejuvenation mycelia, fruiting is effective, and the biological efficiency of third and fourth damp mushroom reaches 70.1%, and total biological efficiency reaches 146.3%.
Embodiment 4: a kind of method for cultivating mushroom, comprises batching, ferments, makes the bed, sows, sends out bacterium, earthing, annesl, management of producing mushroom, concrete operation step:
1. prepare burden: cotton seed hulls 74%, soya-bean cake 17%, mushroom tang 4.5%, lime 3.5%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then the leachate after removing slag is admixed uniformly in the cotton seed hulls through prewetting, soya-bean cake compound, stockpile is become high 1.2m-1.5m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. make the bed, sow, send out bacterium, earthing: the planting material fermented is after heat radiation, and be directly taped against ridge-up bed sowing and send out a bacterium, when mycelia sends out material full, cover the sandy loam that 1cm is thick, moisturizing annesl enters management of producing mushroom;
5. management of producing mushroom: after having adopted the second damp mushroom, the clear water of every square metre of sprinkling 5kg; After having adopted the 3rd damp mushroom, the clear water of every square metre of sprinkling 7kg, the biological efficiency of third and fourth damp mushroom has been 51.2%, and total biological efficiency is 127.4%.
table one mushroom biological efficiency compares
As can be seen from Table I: the ferment effect utilizing mushroom tang leachate to add than single leachate together with the ferment effect that mushroom stems slag adds is good, and output is high; Leachate is good to the effect of rejuvenation of mushroom mycelium, and third and fourth damp mushroom biological efficiency is high, obvious effect of increasing production.
Claims (1)
1. a method for cultivating mushroom, comprises batching, ferments, makes the bed, sows, sends out bacterium, earthing, annesl, management of producing mushroom, it is characterized in that:
(1) prepare burden: cotton seed hulls 72-76%, soya-bean cake 16-18%, mushroom tang 4-5%, lime 3-4%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%; Described mushroom tang is the offcuts in Lentnus edodes process, is ground into the particle of 2mm-3mm after drying;
(2) ferment, make the bed, sow, send out bacterium, earthing: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leachate is admixed in cotton seed hulls, soya-bean cake compound together with mushroom stems slag uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation, after material temperature reaches 60 DEG C, keep 24h, then carry out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%, the planting material fermented, after heat radiation, is directly taped against ridge-up bed sowing and sends out a bacterium, when mycelia sends out material full, cover the sandy loam that 1cm is thick, moisturizing annesl enters management of producing mushroom;
(3) management of producing mushroom: after the second damp mushroom, the 3rd damp mushroom are gathered, sprays the mushroom tang leachate of 0.01-0.02g/ml comprehensively in mushroom bed, every square metre is sprayed 5-7kg; The preparation method of described leachate is: mushroom tang particle is added clear water by the weight ratio of 20 times, soak 18h-24h at normal temperatures, filter and remove residue, then complement to the original water yield, obtain the mushroom tang leachate that concentration is 0.05g/ml, add clear water dilution and use.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510085941.4A CN104584882A (en) | 2015-02-23 | 2015-02-23 | Shiitake cultivation method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510085941.4A CN104584882A (en) | 2015-02-23 | 2015-02-23 | Shiitake cultivation method |
Publications (1)
Publication Number | Publication Date |
---|---|
CN104584882A true CN104584882A (en) | 2015-05-06 |
Family
ID=53111226
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510085941.4A Pending CN104584882A (en) | 2015-02-23 | 2015-02-23 | Shiitake cultivation method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104584882A (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1068699A (en) * | 1991-07-20 | 1993-02-10 | 昆明食用菌研究开发中心 | The method of cultivating mushroom with waste edible fungus cultivation compost |
CN1194089A (en) * | 1998-04-07 | 1998-09-30 | 杭州常青保健食品有限公司 | High temperature resistant mushroom and its breeding and cultivating method |
CN1875682A (en) * | 2006-07-04 | 2006-12-13 | 彭俊 | Stropharia rugoso-annulata plantation method |
CN103214302A (en) * | 2013-03-26 | 2013-07-24 | 河北大学 | Sawdust mushroom residue nutrition agent used for cultivating mozzie buster |
CN103396247A (en) * | 2013-08-01 | 2013-11-20 | 邬金飞 | Shitake mushroom cultivation material compatibility and production method of shitake mushroom cultivation material |
-
2015
- 2015-02-23 CN CN201510085941.4A patent/CN104584882A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1068699A (en) * | 1991-07-20 | 1993-02-10 | 昆明食用菌研究开发中心 | The method of cultivating mushroom with waste edible fungus cultivation compost |
CN1194089A (en) * | 1998-04-07 | 1998-09-30 | 杭州常青保健食品有限公司 | High temperature resistant mushroom and its breeding and cultivating method |
CN1875682A (en) * | 2006-07-04 | 2006-12-13 | 彭俊 | Stropharia rugoso-annulata plantation method |
CN103214302A (en) * | 2013-03-26 | 2013-07-24 | 河北大学 | Sawdust mushroom residue nutrition agent used for cultivating mozzie buster |
CN103396247A (en) * | 2013-08-01 | 2013-11-20 | 邬金飞 | Shitake mushroom cultivation material compatibility and production method of shitake mushroom cultivation material |
Non-Patent Citations (6)
Title |
---|
中国微生物学会病毒专业委员会植物病毒组: "《蔬菜病毒病害及其植物病毒化学防治研究进展:全国第三届蔬菜病毒病害研究机中国植物病毒化学防治研讨会论文选编》", 30 November 1998 * |
何瑞峰: "食用菌下脚料的综合利用", 《今日科技》 * |
李艳莉,刘淑华: "无公害高温双孢菇生产栽培技术规程", 《中国果菜》 * |
杨学云: "白灵菇金针菇下脚料栽培鸡腿菇培养料配方优化试验", 《培养材料》 * |
王谦等: "以香菇柄为主料固态发酵茯苓菌", 《食品科技》 * |
黎德荣: "杏鲍菇下脚料栽培高温蘑菇试验", 《中国食用菌》 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104541995A (en) | Mushroom cultivation method | |
CN104541996A (en) | Pholiota nameko culture method | |
CN104756757A (en) | Method for planting hericium erinaceus | |
CN104584873A (en) | Pleurotus eryngii cultivating method | |
CN104604531A (en) | Cultivation method of Pholiota nameko | |
CN104604529A (en) | Cultivation method of Pholiota nameko | |
CN104737778A (en) | Pleurotus geesteranus cultivation method | |
CN104541994A (en) | Pleurotus nebrodensis culture method | |
CN104737798A (en) | Auricularia polytricha cultivating method | |
CN104737808A (en) | Pleurotus geesteranus cultivation method | |
CN104737787A (en) | Cultivation method of grifola frondosa | |
CN104737807A (en) | Pleurotus geesteranus cultivation method | |
CN104584869A (en) | Hericium erinaceus cultivation method | |
CN104584882A (en) | Shiitake cultivation method | |
CN104737806A (en) | Lentinus edodes cultivation method | |
CN104737804A (en) | Shiitake mushroom cultivation method | |
CN104737776A (en) | Pleurotus eryngii cultivation method | |
CN104782403A (en) | Cultivation method for pholiota nameko | |
CN104782399A (en) | Cultivation method for pholiota nameko | |
CN104737802A (en) | Shiitake mushroom cultivation method | |
CN104782386A (en) | Hypsizygus marmoreus cultivation method | |
CN104604530A (en) | Cultivation method of Pholiota nameko | |
CN104584881A (en) | Pleurotus nebrodensis cultivation method | |
CN104584872A (en) | Pleurotus geesteranus cultivating method | |
CN104782404A (en) | Cultivation method for pholiota nameko |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150506 |
|
RJ01 | Rejection of invention patent application after publication |