CN104560706B - Enzyme reactor, enzymatic reaction system and fat processing method - Google Patents
Enzyme reactor, enzymatic reaction system and fat processing method Download PDFInfo
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- CN104560706B CN104560706B CN201310488714.7A CN201310488714A CN104560706B CN 104560706 B CN104560706 B CN 104560706B CN 201310488714 A CN201310488714 A CN 201310488714A CN 104560706 B CN104560706 B CN 104560706B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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Abstract
This application provides enzyme reactor and enzymatic reaction system, and fat processing method.The enzyme reactor includes：Reaction cavity, it includes immobilised enzymes, and the reaction cavity has charging aperture and drain hole；Pressure-detecting device, the pressure-detecting device detect the pressure of material in the reaction cavity, such as the pressure of the material at charging aperture or near it；Agitating device, it stirs the mixture of immobilised enzymes and material in the reaction cavity；Wherein, the pressure-detecting device communicates to connect with the agitating device, the pressure-detecting device controls the stirring of the agitating device according to the pressure detected by communicating, so as to reduce the degree of packing of immobilised enzymes in the reaction cavity, material is promoted to be flowed out from drain hole, the pressure in release reaction cavity.
Description
Invention field
The application is usually directed to enzyme reactor, enzymatic reaction system and fat processing method.
Background of invention
With the fast development of China's economic, the living standard and quality of the people are significantly improved, and are resolving adequate food and clothing
Consumer has to the attention rate of healthy food and significantly lifted after problem, the grease as one of food three nutritious elements
It is same unexceptional.There is the structure ester of certain physiological function more at this stage（Diglyceride, middle long-chain fat acid glyceride, generation
Cocoa butter etc.）As the focus of scientific research personnel's research.At the same time, international community's environmental pollution, green living are increasingly heavier
Depending on preserving our planet, reducing pollution, the theory of low-carbon environment-friendly is also increasingly received by people, therefore with serious pollution traditional chemical
Production method can be eliminated gradually.With the development of biotechnology, enzyme technology with its reaction condition is gentle, selectivity is high,
The characteristics of catalytic activity is high, environmental pollution is small and the application in oil prodution industry is more and more wider, by numerous researchers
Concern.
At present in oil prodution industry in order to obtain the structure ester with certain function（MLCT, OPO, BOB etc.）Often use enzyme
Method carries out ester exchange.In order to improve efficiency and improve yield, continous way production is typically carried out using reaction column.But in reality
After finding continuous reaction for a period of time in production process, the enzyme in reaction column can press too closely by the pressure of material, make
The rising that pressure can be progressively in Cheng Zhu, material just can not be by reaction column after pressure reaches to a certain degree, therefore also just can not
Produced.Simultaneously because enzyme preparation pressed too closely cause change enzyme preparation it is also cumbersome.
In order to solve this problem, domestic and international scientific research personnel is also designed continous way enzyme process reactor, specific special
Profit is as follows：
A kind of enzyme process reactor is disclosed in CN201120017106.4, is mainly used for solving small kind grease and knot
The production problem of structure ester, it uses pot type batch (-type) to design.
A kind of reactor disclosed in CN200980130518.4, its reactor are that liquid charging stock is mixed and realized
Component is entered in cylinder with constant flow-rate ratio.But the reactor is a kind of Liquid-phase reactor, do not consider there is solid particle
In the presence of pressure rise the problem of.
From the point of view of comprehensive existing patent, as reactor pressure rise causes pipeline to block up in enzymic catalytic reaction continuous production
Fill in, the practical problems such as lipase activity is low do not solve.
Summary of the invention
To overcome deficiency of the prior art in fats and oils processing industry, applicant has developed what is designed for continuous production
Novel production device, its according to reactor pressure, in good time batch type to reduce the degree of packing of immobilised enzymes in reactor,
So as to reduce the pressure of material in reactor so that continuous production can be carried out, and improve the activity of immobilised enzymes simultaneously.This
Outside, applicant further improves enzyme activity by coutroi velocity, temperature, extends the service life of enzyme.
This application discloses enzyme reactor, and it includes：Reaction cavity, it includes immobilised enzymes, and the reaction cavity has
Charging aperture and drain hole；Pressure-detecting device, the pressure-detecting device detects the pressure in the reaction cavity, such as feeds
The pressure of material at mouthful or near it；And agitating device, it stirs the mixing of immobilised enzymes and material in the reaction cavity
Thing；Wherein described pressure-detecting device communicates to connect with the agitating device, and the pressure-detecting device is according to the pressure detected
Power controls the stirring of the agitating device by communicating, and so as to reduce the degree of packing of immobilised enzymes in the reaction cavity, promotees
Enter material to flow out from drain hole, the pressure in release reaction cavity.
On the other hand, this application discloses enzymatic reaction system, it is above-mentioned that it includes head tank, measuring pump, one or more
Enzyme reactor, collecting tank and the pipeline for connecting all parts.
It yet still another aspect, disclosed herein as well is continous way fat processing method, it includes：A. material is made by measuring pump
Continuously enter the reaction cavity of the enzyme reactor containing immobilised enzymes；B. while charging, monitored using pressure-detecting device
The pressure of the mixture of material and immobilised enzymes in the reaction cavity；With c. as the material enters in reaction cavity, lead
Cavity internal pressure is caused to rise, when the pressure reaches 1.5 to 2bar, pressure-detecting device instructs agitating device by communicating
The mixture is stirred, mixing speed is 1-20 revs/min, when the stirring continues until that the pressure is less than 1.5 or 1bar
Stop.
Brief description
Fig. 1 shows an embodiment of the enzyme reactor of the application, and it includes end socket 3-1 and main body 3-2, end socket 3-
Charging aperture 3-7, pressure gauge 3-9, double spiral agitator 3-11 and motor 3-8 are housed on 1；Main body 3-2 is wrapped with jacket heat-preservation
Layer 3-5, jacket heat-preservation layer have water inlet 3-3, delivery port 3-6, and the external heat-exchange devices of heat-insulation layer 3-5 carry out temperature control；End socket 3-1
On the double spiral agitator 3-11 that is equipped be connected by connector and mechanical seal with the motor 3-8 on end socket, double helix stirring
There is double-layer spiral shape blade 3-10 on device 3-11；Reaction cavity 3-2 bottoms are equipped with the screen cloth agreed with completely with reaction cavity, prevent
Only enzyme granulate is spilt, and screen bottom is equipped with drain hole 3-4.
Fig. 2 shows the schematic diagram of the enzymatic reaction system of the application.The system includes head tank 2, measuring pump 6, connection respectively
Pipeline 1, reaction member 3, jacket heat-preservation device 5 and the collecting tank 4 of individual reaction member.
Fig. 3 shows an embodiment of the enzymatic reaction system of the application.System be connected by multiple reaction members 3 and
Into including the pipeline and other pipelines 1 of each reaction member of connection（Pipeline is also jacket heat-preservation）, head tank 2, measuring pump 6,
Jacket heat-preservation device 5, collecting tank 4.Reaction member 3 can be the device of raising enzyme activity as shown in Figure 1, and tandem enzyme is anti-on pipeline
It can be 1-10 to answer device.The number of enzyme reactor is only exemplary in figure, rather than the limitation to the application scope.
Fig. 4 shows the another embodiment of the enzymatic reaction system of the application.System be it is in parallel by multiple enzyme reactors 3 and
Into.Enzyme reactor in parallel can be 1-10 on pipeline.The number of enzyme reactor is only exemplary in figure, rather than to this Shen
Please scope limitation.
Detailed description of the invention
This specification can be read in conjunction with the accompanying, the accompanying drawing should be regarded as the exemplary of the entire written description of the application
Part.Present specification accompanying drawing is only for beneficial to clearly and concisely, some features of the application may be exaggeration in ratio
Or how many show in schematic form.In this manual, relative term such as " on ", " under ", " top " and " bottom
Portion " and its derivative words should be interpreted to refer to according to direction described at that time or the direction as illustrated in the drawing under discussion.This
A little relative terms are for convenience of description and not usually intention requires specific direction.Term on connection refers to
The relation wherein either directly or indirectly fixed or connected each other by intermediate structure, and it is moveable or be rigidly connected or
Both relations, unless separately making especially expression.
In the following description, some details are set forth, to provide the deep understanding to various embodiments.
However, it is understood by those of ordinary skill in the art that the present invention can be implemented in the case of without these details.In other situations
In, structure known to detailed displaying or detailed description, to avoid the ambiguous description unnecessary to embodiment.
" embodiment " mentioned in entire disclosure or " embodiment " refer to related to described embodiment
Specific features, structure or characteristic be contained within least one embodiment.Therefore, entire disclosure goes out in different places
Existing phrase " in one embodiment " or " in embodiments " might not be all referring to same embodiments.In addition,
Specific feature, structure or characteristic can combine in any suitable manner in one or more embodiments.Therefore, at this
In application, and the achievable all technique effects listed herein of not all embodiment.Preferably, every kind of embodiment should be real
Show at least one technique effect, or it can realize the combination of multiple technologies effect.
In addition, unless the context clearly dictates otherwise, as used in this specification and the appended claims book, odd number shape
The indefinite article " one (a) " of formula, " a kind of (an) " and " (the) " include a plurality of objects.It is also noted that
Unless the context clearly dictates otherwise, term "or" generally comprises the meaning of "and/or".
Terms used herein " Lipozyme RM IM " or " Lipozyme TL IM " are the trade names of immobilized lipase
Claim, they are purchased from letter enzyme preparation company of Novi of Denmark.
In a first aspect, the application is related to enzyme reactor, it includes：Reaction cavity, it includes immobilised enzymes, the reaction
Cavity has charging aperture and drain hole；Pressure-detecting device, the pressure-detecting device detect material in the reaction cavity
The pressure of material at pressure, such as charging aperture or near it；And agitating device, it stirs immobilised enzymes in the reaction cavity
With the mixture of material；Wherein described pressure-detecting device communicates to connect with the agitating device, the pressure-detecting device root
The stirring of the agitating device is controlled by communicating according to the pressure detected, so as to reduce immobilised enzymes in the reaction cavity
The degree of packing, promote material to be flowed out from drain hole, the pressure in release reaction cavity.
In the production process of grease, in order to realize the purpose of continuous production, grease is continuously pumped into enzyme reactor,
Grease slowly flows across the enzyme reactor equipped with enzyme preparation to complete to react from top to down.As the production time extends, enzyme preparation
Grain is gradually compacted by liquid stream, and reactor pressure gradually increases, and until pipeline blockage, grease can not be by have impact on reaction
It is carried out continuously, and the vigor and service life of enzyme, reduce production efficiency.Pressure in reactor is immobilised enzymes to material
Resistance.Therefore applicant is mounted with pressure-detecting device and agitating device on enzyme reactor, when pressure-detecting device detects
When reaching a certain threshold value to the pressure of liquid stream, it sends signal, and notice agitating device starts to stir.With this, enzyme system can be prevented
Agent is pressed excessively tightly to cause grease not pass through.Meanwhile this batch type also avoid and continuously stir to enzyme preparation
Destruction, improve the enzyme activity during continuous production, extend the service life of enzyme.Pass through at least both factors, the application
Enzyme reactor realize efficient continuous production.
In some embodiments, reaction cavity includes main body and the end socket installed in the body top.In some realities
Apply in scheme, charging aperture is arranged on end socket；In some embodiments, pressure-detecting device and the charging aperture and it is listed in institute
State on end socket.
In some embodiments, drain hole is located at the bottom part body, alternatively before the drain hole be equipped with
The screen cloth that reaction cavity agrees with, to intercept the enzyme preparation loaded in main body.
In some embodiments, enzyme reactor also includes temperature control equipment.In some embodiments, temperature control
Device can be the heater or refrigerator for having temperature sensor, or jacket heat-preservation layer.In some embodiments, temperature control
Device processed is the jacket heat-preservation layer with water inlet and delivery port, alternatively the jacket heat-preservation layer connection heat-exchange device.
In some embodiments, the delivery port and water inlet of heat-exchange device connect with the water inlet and delivery port of jacket heat-preservation layer respectively
Connect.In some embodiments, temperature control equipment is alternatively controlled by the temperature control in the enzyme reactor at 50-80 DEG C
System is at 60-70 DEG C.
In some embodiments, pressure-detecting device includes pressure gauge and pressure sensitive unit.
In some embodiments, agitating device is motor-driven double spiral agitator, the double spiral agitator tool
There is double-layer spiral shape blade.In some embodiments, double spiral agitator passes through connector and mechanical seal with the electrode
Connection.
In some embodiments, when the pressure reaches 1.5 to 2bar, the pressure-detecting device is to the stirring
Device sends communication, instructs the agitating device to start to stir, and mixing speed is 1-20 revs/min, stirs to pressure and is less than 1.5
Or stop during 1.0bar, or mixing time is 1-10 minutes, alternatively the mixing speed is 10-15 revs/min, alternatively
For 10 revs/min；Alternatively the mixing time is 5-8 minutes, is optionally 5 minutes.Mixing speed and mixing time for
The integrity degree and activity of enzyme granulate have a certain impact, if mixing time is long, mixing speed is too fast, then some enzyme granulates may
Damaged, disintegration, causes enzyme activity to reduce.
Fig. 1 shows an embodiment of the enzyme reactor of the application, and it includes end socket 3-1 and main body 3-2, end socket 3-
Charging aperture 3-7, pressure gauge 3-9, double spiral agitator 3-11 and motor 3-8 are housed on 1；Main body 3-2 is wrapped with jacket heat-preservation
Layer 3-5, jacket heat-preservation layer have water inlet 3-3, delivery port 3-6, and the external heat-exchange devices of heat-insulation layer 3-5 carry out temperature control；End socket 3-1
On the double spiral agitator 3-11 that is equipped be connected by connector and mechanical seal with the motor 3-8 on end socket, double helix stirring
There is double-layer spiral shape blade 3-10 on device 3-11；Reaction cavity 3-2 bottoms are equipped with the screen cloth agreed with completely with reaction cavity, prevent
Only enzyme granulate is spilt, and screen bottom is equipped with drain hole 3-4.
In second aspect, the application is related to enzymatic reaction system, and it includes head tank, measuring pump, one or more above-mentioned enzymes
Reactor, collecting tank and the pipeline for connecting all parts.
In some embodiments, the multiple enzyme reactor be series connection and/or it is in parallel.In some embodiments
In, the multiple enzyme reactor is series connection.In some embodiments, the multiple enzyme reactor is series connection.At some
In embodiment, the multiple enzyme reactor is that connection in series-parallel mixes, such as the series connection of multiple enzyme reactors turns into enzyme reactor group,
Then the enzyme reactor group connected is further in parallel；Or multiple enzyme reactors are in parallel turns into enzyme reactor group, then parallel connection
Enzyme reactor group is further connected.In some embodiments, alternatively the quantity of the enzyme reactor is 1-10.Enzyme reaction
Enzyme reactor in system can be multiple series connection, improve reaction product yield；It can also be that multiple enzyme reactors are in parallel, improve
Yield；Either multiple enzyme reactor connection in series-parallel are carried out simultaneously, both improve yield, improve yield again.
In some embodiments, enzyme reactor includes：Reaction cavity, it includes immobilised enzymes, the reaction cavity tool
There are charging aperture and drain hole；Pressure-detecting device, the pressure-detecting device detect the pressure of material in the reaction cavity, example
Such as the pressure of the material at charging aperture or near it；And agitating device, it stirs immobilised enzymes and material in the reaction cavity
Mixture；Wherein described pressure-detecting device and the agitating device communicate to connect, and the pressure-detecting device is according to being examined
The pressure of survey controls the stirring of the agitating device by communicating, so as to reduce the consolidation of immobilised enzymes in the reaction cavity
Degree, material is promoted to be flowed out from drain hole, the pressure in release reaction cavity.
In some embodiments, reaction cavity includes main body and the end socket installed in the body top.In some realities
Apply in scheme, charging aperture is arranged on end socket；In some embodiments, pressure-detecting device and the charging aperture and it is listed in institute
State on end socket.
In some embodiments, drain hole is located at the bottom part body, alternatively before the drain hole be equipped with
The screen cloth that reaction cavity agrees with, to intercept the enzyme preparation loaded in main body.
In some embodiments, enzyme reactor also includes temperature control equipment.In some embodiments, temperature control
Device can be the heater or refrigerator for having temperature sensor, or jacket heat-preservation layer.In some embodiments, temperature control
Device processed is the jacket heat-preservation layer with water inlet and delivery port, alternatively the jacket heat-preservation layer connection heat-exchange device.
In some embodiments, the delivery port and water inlet of heat-exchange device connect with the water inlet and delivery port of jacket heat-preservation layer respectively
Connect.In some embodiments, temperature control equipment is alternatively controlled by the temperature control in the enzyme reactor at 50-80 DEG C
System is at 60-70 DEG C.
In some embodiments, pressure-detecting device includes pressure gauge and pressure sensitive unit.
In some embodiments, agitating device is motor-driven double spiral agitator, the double spiral agitator tool
There is double-layer spiral shape blade.In some embodiments, double spiral agitator passes through connector and mechanical seal with the electrode
Connection.
In some embodiments, when the pressure reaches 1.5 to 2bar, the pressure-detecting device is to the stirring
Device sends communication, instructs the agitating device to start to stir, and mixing speed is 1-20 revs/min, stirs to pressure and is less than 1.5
Or stop during 1.0bar, or mixing time is 1-10 minutes, alternatively the mixing speed is 10-15 revs/min, alternatively
For 10 revs/min；Alternatively the mixing time is 5-8 minutes, is optionally 5 minutes.Mixing speed and mixing time for
The integrity degree and activity of enzyme granulate have a certain impact, if mixing time is long, mixing speed is too fast, then some enzyme granulates may
Damaged, disintegration, causes enzyme activity to reduce.
In the third aspect, the application is related to continous way fat processing method, and it includes：A. make material continuous by measuring pump
Into the reaction cavity of the above-mentioned enzyme reactor containing immobilised enzymes；B. while charging, monitored using pressure-detecting device
The pressure of the mixture of material and immobilised enzymes in the reaction cavity；With c. as the material enters in reaction cavity, lead
Cavity internal pressure is caused to rise, when the pressure reaches 1.5 to 2bar, pressure-detecting device instructs agitating device by communicating
The mixture is stirred, mixing speed is 1-20 revs/min, when the stirring continues until that the pressure is less than 1.5 or 1bar
Stop.In some embodiments, alternatively the mixing speed is 10-15 revs/min, is optionally 10 revs/min.One
In a little embodiments, mixing time is 1 to 10 minute, and alternatively the mixing time is 5-8 minutes, is optionally 5 minutes.
In some embodiments, the flow velocity that methods described passes through enzyme reactor using the measuring pump control material
For 1 to 3 times per hour dress enzyme amount, alternatively 1 to 2 times fills enzyme amount, alternatively 1.5 to 2 times of dress enzyme amount.Multiple as described herein is
Refer to weight ratio, i.e., pass through the ratio between immobilised enzymes gross weight in the weight and enzyme reactor of the material of enzyme reactor per hour.One
In a little embodiments, methods described includes controlling the temperature of the enzyme reactor to be about 50 to 80 DEG C, alternatively 50 to 70 DEG C, can
60 to 70 DEG C of selection of land.
In some embodiments, the temperature of the enzyme reactor is about 50 DEG C, 51 DEG C, 52 DEG C, 53 DEG C, 54 DEG C, 55 DEG C,
56 DEG C, 57 DEG C, 58 DEG C, 59 DEG C, 60 DEG C, 61 DEG C, 62 DEG C, 63 DEG C, 64 DEG C, 65 DEG C, 66 DEG C, 67 DEG C, 68 DEG C, 69 DEG C, 70 DEG C, 71
DEG C, 72 DEG C, 73 DEG C, 74 DEG C, 75 DEG C, 76 DEG C, 77 DEG C, 78 DEG C, 79 DEG C, or 80 DEG C.
In some embodiments, the immobilised enzymes is Lipozyme RM IM or Lipozyme TL IM.
In some embodiments, methods described is carried out using above-mentioned enzyme reactor or enzymatic reaction system.
Embodiment
In following embodiments of the present invention, the soybean oil, oleic acid, palm oil, corn oil, the rapeseed oil that use pass through city
Field purchase obtains；Medium chain triglyceride specification is C8/C10=50/50, w/w, purchased from beneficial sea（Lianyun Harbour）The limited public affairs of oiling industry
Department.
In following embodiments of the present invention, the NMR used is purchased from Brooker spectrometer for MINISPEC MQ20
Device company.
Ester exchange enzyme activity determination method is as follows in following examples：
By soybean oil and deep hydrogenation soybean oil with 73:27 (w/w) ratio mixing, weigh 10g and have plug triangle in 100ml
In bottle, 0.5g immobilised enzymes is added, 150rpm reacts 30min in 70 DEG C of shaking baths.Portion of product is taken out after reaction to put
Enter in solid fat pipe, the solid fat pipe equipped with product is put into 15min in 100 DEG C of baking ovens, be sequentially placed into 5min in 60 DEG C of water-baths, 0
DEG C water-bath 60min, 30min in 40 DEG C of water-baths, water-bath are immediately inserted into preheated NMR measurement SFC and contained after terminating
Amount.Changed with product SFC to reflect enzyme activity, with unreacted material（With 73:27 (w/w) ratio mixing soybean oil and
Deep hydrogenation soybean oil）As blank control, enzyme activity calculation formula is IUN（interesterification unit NOVO）=
(SFC sample-SFC blank)/30 × 1260.At the beginning of Lipozyme TL IM enzymes initial enzyme activity 393IUN/g, Lipozyme RM IM
Beginning enzyme activity 350IUN/g, the two is purchased from letter enzyme preparation company of Novi of Denmark.
Embodiment 1：Enzyme reactor
Enzyme reactor, as shown in figure 1, including the end socket 3-1 being connected with reaction cavity 3-2, charging aperture is housed on end socket 3-1
3-7, pressure gauge 3-9, double spiral agitator 3-11, motor 3-8；Reaction cavity 3-2 is wrapped with jacket heat-preservation layer 5, and chuck is protected
Warm layer 3-5 has water inlet 3-3, delivery port 3-6, and the external heat-exchange devices of heat-insulation layer 3-5 carry out temperature control；Double spiral agitator 3-11
It is connected by connector and mechanical seal with motor 3-8, there is double-layer spiral shape blade 3-10 on double spiral agitator 3-11；Reaction
Cavity 3-2 bottoms are equipped with the screen cloth agreed with completely with reaction cavity 3-2, prevent enzyme granulate from spilling（It is not shown）, screen bottom dress
There is drain hole 3-4.
When pressure reaches the stirring startup pressure of setting in enzyme reactor（Such as 1.5 to 2bar）When, pressure-detecting device 3-9
Communication is sent to agitating device 3-11, indicates that the agitating device 3-9 starts to stir, when stirring of the pressure less than setting stops pressure
Power（Such as it is less than 1.5bar or 1bar）When, pressure-detecting device 3-9 sends communication to agitating device 3-11, indicates the stirring dress
Put 3-9 and stop stirring.Mixing speed can be set as needed, such as can be 1-20 revs/min, and mixing time is 1-10 points
Clock.
Embodiment 2：Enzymatic reaction system
As shown in Fig. 2 enzymatic reaction system is by head tank 2, measuring pump 6, connecting line 1（Jacket heat-preservation）, enzyme reactor 3,
Jacket heat-preservation device 5, collecting tank 4 form.
Embodiment 3：Enzymatic reaction system
As shown in figure 3, enzymatic reaction system is in series by multiple enzyme reactors 3, in addition to head tank 2, measuring pump 6,
Jacket heat-preservation device 5, collecting tank 4, connecting line 1（Jacket heat-preservation）Deng part.The enzyme reactor 3 connected on pipeline can be 1-
10.
Embodiment 4：Enzymatic reaction system
As shown in figure 4, enzymatic reaction system is formed in parallel by multiple enzyme reactors 3, in addition to head tank 2, measuring pump 6,
Jacket heat-preservation device 5, collecting tank 4, connecting line 1（Jacket heat-preservation）Deng part.Enzyme reactor in parallel can be 1- on pipeline
10.
Embodiment 5
By soybean oil and medium chain triglyceride using weight ratio as 6：After 1 ratio is well mixed, the enzyme for loading embodiment 2 is anti-
In the head tank for answering system, with the flow velocity of 2 times of enzyme amount per hour slowly by with the addition of 10wt% raw material gross weight immobilized lipases
The packed column of enzyme, ester exchange reaction is carried out at 60 DEG C.When pressure gauge reaches 2bar on enzyme reactor, start to stir, agitator
Rotating speed is 10r/min, mixing time 5min, and pressure drops to 1bar, stops stirring.
Continuous production 6 days, enzyme reactor top, middle, bottom enzyme activity are analyzed respectively, meanwhile, not carry out
The experiment of stirring the results are shown in Table 1 as control.
Embodiment 6
By oleic acid and palm oil using weight ratio as 2：After 1 ratio is well mixed, load the enzymatic reaction system of embodiment 3
In head tank, with the flow velocity of 1.5 times of enzyme amount per hour slowly by with the addition of 10wt% raw material gross weight immobilized lipases
The packed column of (Lipozyme RM IM), 70 DEG C of progress ester exchange reactions, when pressure gauge reaches 1.5bar on enzyme reactor, is opened
Begin to stir, agitator speed 10r/min, mixing time 5min, pressure drop to 1bar, stop stirring.
Continuous production 6 days, enzyme reactor top, middle, bottom enzyme activity are analyzed respectively, meanwhile, not carry out
The experiment of stirring the results are shown in Table 1 as control.
The enzyme activity comparative test result of TL enzymes and RM enzymes after with or without continuous reaction 6d under stirring of table 1.
Embodiment 7
By corn oil and medium chain triglyceride using weight ratio as 3：After 1 ratio is well mixed, the enzyme for loading embodiment 4 is anti-
In the head tank for answering system, with the flow velocity of 2 times of enzyme amount per hour slowly by with the addition of the immobilization fat of 10wt% raw material gross weights
Fat enzyme (Lipozyme TL IM) packed column, 60 DEG C of progress ester exchange reactions, when pressure gauge reaches 2bar on enzyme reactor, is opened
Begin to stir, agitator speed is 10r/min mixing time 5min, and pressure drops to 1bar, stops stirring.
Continuous production 3 days, observes the graininess of lipase in enzyme reactor, while tests mixing speed and be respectively
To the influence of lipase enzyme granule when 5r/min, 20r/min, the results are shown in Table 2. experiments using mixing speed as 25r/min is control,
It the results are shown in Table 2.
The mixing speed of table 2. is to enzyme granulate comparative test result
Embodiment 8
By rapeseed oil and medium chain triglyceride using weight ratio as 2：After 1 ratio is well mixed, the enzyme for loading embodiment 2 is anti-
In the head tank for answering system, with the flow velocity of 2 times of enzyme amount per hour slowly by with the addition of 10wt% raw material gross weight immobilized lipases
Enzyme (Lipozyme TL IM) packed column, 60 DEG C of progress ester exchange reactions, when pressure gauge reaches 2bar on enzyme reactor, starts
Stirring, agitator speed 10r/min, mixing time 5min, pressure drop to 1bar, stop stirring.
Continuous production 6 days, enzyme reactor top, middle, bottom enzyme activity are analyzed respectively.With 4 times per hour streams
Experiment of the flow velocity of amount by with the addition of 10wt% raw material gross weights immobilized lipase (Lipozyme TL IM) packed column is used as
Control, the results are shown in Table 3.
Embodiment 9
By rapeseed oil and medium chain triglyceride with weight than 2:After 1 ratio is well mixed, load the enzyme reaction of embodiment 2
In the head tank of system, with the flow velocity of 3 times of enzyme amount per hour slowly by with the addition of 10wt% raw material gross weight lipase
(Lipozyme TL IM) packed column, 60 DEG C of progress ester exchange reactions, when pressure gauge reaches 2bar on enzyme reactor, starts to stir
Mix, agitator speed 10r/min, mixing time 5min.Continuous production is after 6 days respectively to enzyme reactor top, middle, bottom
Portion's enzyme activity is analyzed, and the results are shown in Table 3.
Influence comparative test result of the flow velocity of table 3. to enzyme activity
Embodiment 10
By rapeseed oil and medium chain triglyceride using weight ratio as 6：After 1 ratio is well mixed, the enzyme for loading embodiment 3 is anti-
In the head tank for answering system, with the flow velocity of 2 times of enzyme amount per hour slowly by with the addition of 10wt% raw material gross weight immobilized lipases
Enzyme (Lipozyme TL IM) packed column, respectively with 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C of progress ester exchange reactions, when on enzyme reactor
When pressure gauge reaches 2bar, starting to stir, agitator speed 10r/min, mixing time 5min, pressure drops to 1.5bar,
Stop stirring.
Continuous production 3 days, enzyme reactor top, middle, bottom enzyme activity are analyzed respectively, the results are shown in Table 4.
Influence comparative test result of the temperature of table 4. to enzyme activity
Above result of the test shows, uses foregoing coutroi velocity, temperature, the method and apparatus stirred, Ke Yi great
It is big to improve continuous production enzyme activity, extend the service life of enzyme, pressure is constant in guarantee system, effectively prevent pipeline blockage,
Whole process saves, efficiently, ensures being carried out continuously for production.
This application discloses the general description of involved invention and multiple embodiments or scheme.But it must not incite somebody to action
Invention involved by the application is limited only to these general descriptions and disclosed embodiment or scheme.According to the application's
Open, those skilled in the art can carry out appropriate adjustment to the specific embodiment or scheme, such as will not
Reconfigured with the particular technique feature in embodiment, form other embodiments for still falling within the inventive concept.
In addition, relevant technical scheme described herein can also arbitrarily include program, therefore, involved relevant technical scheme can be made
Combination for hardware and program is individually performed with hardware.
Claims (71)
1. enzyme reactor, it includes：
Reaction cavity, it includes immobilised enzymes, and the reaction cavity has charging aperture and drain hole；
Pressure-detecting device, the pressure-detecting device detect the pressure of material in the reaction cavity；
Agitating device, it stirs the mixture of immobilised enzymes and material in the reaction cavity；
Wherein, the pressure-detecting device and the agitating device communicate to connect, and the pressure-detecting device is according to being detected
Pressure controls the stirring of the agitating device by communicating, so as to reduce the degree of packing of immobilised enzymes in the reaction cavity,
Material is promoted to be flowed out from drain hole, the pressure in release reaction cavity.
2. enzyme reactor as claimed in claim 1, wherein the pressure of the material is the material at charging aperture or near it
Pressure.
3. enzyme reactor as claimed in claim 1 or 2, wherein the reaction cavity includes main body and installed in the main body top
The end socket in portion.
4. enzyme reactor as claimed in claim 3, wherein the charging aperture is arranged on the end socket.
5. enzyme reactor as claimed in claim 4, wherein the pressure-detecting device and the charging aperture and being listed in the envelope
On head.
6. enzyme reactor as claimed in claim 1 or 2, wherein the pressure-detecting device includes pressure gauge and pressure sensitive list
Member.
7. enzyme reactor as claimed in claim 1 or 2, wherein the immobilised enzymes is Lipozyme RM IM or Lipozyme
TL IM。
8. enzymatic reaction system, it is anti-that it includes head tank, measuring pump, the enzyme any one of one or more claim 1-7
Answer device, collecting tank and the pipeline for connecting all parts.
9. enzymatic reaction system as claimed in claim 8, wherein the multiple enzyme reactor is series connection and/or in parallel.
10. enzymatic reaction system as claimed in claim 8 or 9, wherein the quantity of the enzyme reactor is 1-10.
11. continous way fat processing method, it includes：
A. material is made to continuously enter the reaction chamber of the enzyme reactor containing immobilised enzymes any one of claim 1-7
Body；
B. pressure in the reaction cavity is monitored using pressure-detecting device；With
C. when the pressure reaches 1.5 to 2bar, it is described mixed that pressure-detecting device instructs agitating device to stir by communicating
Compound, mixing speed are 5-20 revs/min, and the stirring continues until to be stopped when the pressure is less than 1.5 or 1bar.
12. method as claimed in claim 11, wherein making material add the reaction cavity by measuring pump.
13. the method as described in claim 11 or 12, wherein the mixing speed is 10-15 revs/min.
14. the method as described in claim 11 or 12, wherein the mixing time is 1 to 10 minute.
15. method as claimed in claim 13, wherein the mixing time is 1 to 10 minute.
16. the method as described in claim 11 or 12, wherein the mixing time is 5-8 minutes.
17. method as claimed in claim 13, wherein the mixing time is 5-8 minutes.
18. such as the method any one of claim 11 to 12,15 and 17, wherein methods described is also using measuring pump
The material is controlled to enter the flow velocity of the reaction cavity of the enzyme reactor.
19. method as claimed in claim 13, wherein methods described also control the material to enter institute using measuring pump
State the flow velocity of the reaction cavity of enzyme reactor.
20. method as claimed in claim 14, wherein methods described also control the material to enter institute using measuring pump
State the flow velocity of the reaction cavity of enzyme reactor.
21. method as claimed in claim 16, wherein methods described also control the material to enter institute using measuring pump
State the flow velocity of the reaction cavity of enzyme reactor.
22. method as claimed in claim 18, wherein the flow velocity is 1 to 3 times per hour dress enzyme amount.
23. the method as any one of claim 19 to 21, wherein the flow velocity is 1 to 3 times per hour dress enzyme amount.
24. method as claimed in claim 18, wherein the flow velocity is 1 to 2 times of dress enzyme amount.
25. the method as any one of claim 19 to 21, wherein the flow velocity is 1 to 2 times of dress enzyme amount.
26. method as claimed in claim 18, wherein the flow velocity is 1.5 to 2 times of dress enzyme amount.
27. the method as any one of claim 19 to 21, wherein the flow velocity is 1.5 to 2 times of dress enzyme amount.
28. such as the method any one of claim 11,12,15,17,19-22,24 and 26, wherein methods described includes
The temperature for controlling the enzyme reactor is 50 to 80 DEG C.
29. method as claimed in claim 13, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 80
℃。
30. method as claimed in claim 14, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 80
℃。
31. method as claimed in claim 16, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 80
℃。
32. method as claimed in claim 18, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 80
℃。
33. method as claimed in claim 23, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 80
℃。
34. method as claimed in claim 25, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 80
℃。
35. method as claimed in claim 27, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 80
℃。
36. such as the method any one of claim 11,12,15,17,19-22,24 and 26, wherein methods described includes
The temperature for controlling the enzyme reactor is 50 to 70 DEG C.
37. method as claimed in claim 13, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 70
℃。
38. method as claimed in claim 14, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 70
℃。
39. method as claimed in claim 16, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 70
℃。
40. method as claimed in claim 18, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 70
℃。
41. method as claimed in claim 23, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 70
℃。
42. method as claimed in claim 25, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 70
℃。
43. method as claimed in claim 27, wherein methods described include controlling the temperature of the enzyme reactor to be 50 to 70
℃。
44. such as the method any one of claim 11,12,15,17,19-22,24 and 26, wherein methods described includes
The temperature for controlling the enzyme reactor is 60 to 70 DEG C.
45. method as claimed in claim 13, wherein methods described include controlling the temperature of the enzyme reactor to be 60 to 70
℃。
46. method as claimed in claim 14, wherein methods described include controlling the temperature of the enzyme reactor to be 60 to 70
℃。
47. method as claimed in claim 16, wherein methods described include controlling the temperature of the enzyme reactor to be 60 to 70
℃。
48. method as claimed in claim 18, wherein methods described include controlling the temperature of the enzyme reactor to be 60 to 70
℃。
49. method as claimed in claim 23, wherein methods described include controlling the temperature of the enzyme reactor to be 60 to 70
℃。
50. method as claimed in claim 25, wherein methods described include controlling the temperature of the enzyme reactor to be 60 to 70
℃。
51. method as claimed in claim 27, wherein methods described include controlling the temperature of the enzyme reactor to be 60 to 70
℃。
52. such as the method any one of claim 11,12,15,17,19-22,24 and 26, wherein the immobilised enzymes
For Lipozyme RM IM or Lipozyme TL IM.
53. method as claimed in claim 13, wherein the immobilised enzymes is Lipozyme RM IM or Lipozyme TL
IM。
54. method as claimed in claim 14, wherein the immobilised enzymes is Lipozyme RM IM or Lipozyme TL
IM。
55. method as claimed in claim 16, wherein the immobilised enzymes is Lipozyme RM IM or Lipozyme TL
IM。
56. method as claimed in claim 18, wherein the immobilised enzymes is Lipozyme RM IM or Lipozyme TL
IM。
57. method as claimed in claim 23, wherein the immobilised enzymes is Lipozyme RM IM or Lipozyme TL
IM。
58. method as claimed in claim 25, wherein the immobilised enzymes is Lipozyme RM IM or Lipozyme TL
IM。
59. method as claimed in claim 27, wherein the immobilised enzymes is Lipozyme RM IM or Lipozyme TL
IM。
60. as any one of claim 11,12,15,17,19-22,24,26,29-35,37-43,45-51 and 53-59
Method, wherein methods described usage right require 8-10 any one of enzymatic reaction system carry out.
61. method as claimed in claim 13, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
62. method as claimed in claim 14, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
63. method as claimed in claim 16, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
64. method as claimed in claim 18, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
65. method as claimed in claim 23, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
66. method as claimed in claim 25, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
67. method as claimed in claim 27, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
68. method as claimed in claim 28, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
69. method as claimed in claim 36, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
70. method as claimed in claim 44, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
71. method as claimed in claim 52, wherein methods described usage right require that the enzyme any one of 8-10 is anti-
System is answered to carry out.
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