CN104535770A - Myoglobin determination kit of compound antibody - Google Patents
Myoglobin determination kit of compound antibody Download PDFInfo
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- CN104535770A CN104535770A CN201410782871.3A CN201410782871A CN104535770A CN 104535770 A CN104535770 A CN 104535770A CN 201410782871 A CN201410782871 A CN 201410782871A CN 104535770 A CN104535770 A CN 104535770A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6887—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from muscle, cartilage or connective tissue
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
- G01N2333/805—Haemoglobins; Myoglobins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/32—Cardiovascular disorders
- G01N2800/324—Coronary artery diseases, e.g. angina pectoris, myocardial infarction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/50—Determining the risk of developing a disease
Abstract
The invention provides a myoglobin determination kit of a compound antibody. The myoglobin determination kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a reaction liquid for promoting antigen specific binding of the antibody; the reagent R2 is a buffer solution of nano latex micro-particles combined with an anti-human-myoglobin antibody; and the reagent R2 is characterized by being prepared by virtue of the following steps: coupling polystyrene nano latex micro-particles of which the diameters are 50-300nm with a myoglobin monoclonal antibody or a polyclonal antibody to obtain conjugates with different particle sizes and different antibodies; and mixing the conjugates with different particle sizes and different antibodies according to different proportions to prepare the reagent R2. The reagent R2 can be used for detecting myoglobin in human serum, and can be used for improving the detection sensitivity and linear range of the kit provided by the invention. The detection sensitivity of the kit is 0.2mu g/L, and the linear range of the kit is 0-1200mu g/L.
Description
Technical field
The invention belongs to field of medical examination, the myoglobins being specifically related to a kind of nano rubber latex particle type measures kit.
Background technology
Myoglobins (Mbglobin, Mb) is a heme albumen with 153 amino acid whose polypeptied chains and an iron content prosthetic heme group composition, and relative molecular mass 17500 is mainly distributed in striated muscle (cardiac muscle and skeletal muscle) cell.Because molecular weight is little, during cardiac muscle cells damage, Mb is the best early sign thing entering blood.Mb, by glomerular filtration, discharges from urine, and the concentration therefore measuring Mb in serum and urine can be used for some myopathy and cardiopathic diagnosis.As acute myocardial infarction AMI, acute or chronic renal failure, serious congestive heart failure, suffer a shock for a long time, the myopathy that neuromuscular disease and a variety of causes cause.
Acute myocardial infarction AMI (AMI) is the acute myocardial injury disease of clinical common a kind of high case fatality rate, at the initial stage of a disease, Mb is released in blood at first, after symptom occurs about 1 ~ 3 hour, in blood, Mb can exceed upper limits of normal, within 9 ~ 12 hours, reach peak value, after 24 ~ 36 hours, recover normal, measure the sensitiveest early stage index that serum Mb can be used as AMI diagnosis.Due to Mb half life period short (15min), within 6 ~ 12 hours after episode, do not raise, then can get rid of the diagnosis of AMI; Mb is very fast after AMI to be removed from kidney, fall ill in 18 ~ 30 hours and can return to normal level completely, therefore the mensuration of Mb contributes to observing in the AMI course of disease and expands with or without infarct or infarct more again, if increasing frequently appears in Mb, point out original myocardial infarction still in continuity.
The method of related detection serum myoglobin mainly adopts immunological method, with enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA), radioimmunoassay (radioimmunoassay, RIA), chemoluminescence method (Chemiluminescence, CL) and particle reinforce transmission immunological turbidimetry method (particle-enhanced turbidimetric immunoassay, PETIA) this several method be main.Although ELISA method employs recent two decades clinically, still there are some fatal defects in it, and dosing accuracy is poor, the running time is long, automaticity is low, generally can only be used for qualitative detection.RIA is highly sensitive, but unstable, and repeatability is poorer than ELISA, and there is alpha-contamination danger.CL is expensive, needs special instrument and professional's operation, is unfavorable for that basic hospital is popularized.PETIA be occur in recent years a kind of comparatively stable, fast, the detection method of Accurate Determining myoglobins serum-concentration.The ultimate principle of PETIA is the surface-crosslinked monoclonal antibody at macromolecule glue lactoconium, when crosslinked have the particulate of antibody to be combined with antigen after, can flock together rapidly at short notice, change the light transmission of reactant liquor.And, the change of reactant liquor light transmission (i.e. absorbance) and the concentration of tested antigen have stronger correlativity, and namely latex particle agglutination is more, more can hinder the light transmission of light, the absorbance of reactant liquor is larger, is directly proportional within the specific limits to the concentration of tested antigen.The PETIA detection method reaction time is short, and precision is good, and sensing range is wide, jaundice, haemolysis and piarhemia sample are all unaffected, being easy to robotization, be a kind of detection method used at present widely, but the detection sensitivity of the method and the range of linearity need further raising.
At present, the latex particle that PETIA detection reagent place adopts mostly is inert particulate, carboxylated particulate and amination particulate.The latex particle of finishing and the combination of antibody are by the carboxyl on its surface or aminoterminal covalent bond that is amino and antibody; a bridging chemistry arm is had between particulate and antibody; reduce steric effect; not only increase the Percentage bound of antibody; but also provide suitable three dimensions spatial structure for antibody, effectively protect the active region that antibody is combined with antigen.Have the particulate particles raw material that many companies provide Nanoscale Surface to modify both at home and abroad, and be widely used in PETIA.The particle diameter that these producers provide, can as the carrier of polyclonal antibody and monoclonal antibody in 20nm ~ 4 μm.The release of nanometer particle solve to monoclonal antibody combine indifferent, the problems such as the range of linearity of clinical detection is narrow, and sensitivity is low, and disturbing factor is many, stable experiment difference, facilitate immunoturbidimetry widespread use clinically greatly.
Summary of the invention
[0006]technical matters to be solved by this invention improves the sensitivity for analysis and sensing range that detect myoglobin content in serum further; There is provided a species specificity good, highly sensitive, precision is good, the nano rubber latex particle type myoglobins mensuration kit that accuracy is high.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of myoglobins of compound antibody measures kit, and comprise reagent R1 and reagent R2, wherein the volume ratio of reagent R1 and reagent R2 is 3 ︰ 1.Described reagent R1 be enhancing antibody antigentic specificity combine reactant liquor, component comprises: the damping fluid of 10 ~ 30mmol/L, 0.01% ~ 4%(w/v) increasing milk agent, 0.05% ~ 2%(w/v) stabilizing agent, 0.1%(w/v) antiseptic; Described reagent R2 is the damping fluid being combined with anti-human myoglobins antibody nano rubber latex particulate, and component comprises: 0.1% ~ 0.5%(w/v) be marked with nano rubber latex particulate, the 0.02% ~ 2%(w/v of anti-human myoglobins antibody) stabilizing agent, the damping fluid of 10 ~ 30mmol/L, 0.1%(w/v) antiseptic.
Described damping fluid is one or more in TRIS buffer, the horizontal acid of 2-morpholine second (NES) buffer solution, phosphate buffer, borate buffer solution, glycine buffer, hydroxyethyl piperazine second thiosulfonic acid (HEPES) damping fluid and trishydroxymethylaminomethane (Tris) damping fluid; Described pH of cushioning fluid is 6 ~ 9.Described damping fluid can have the damping fluid of similar features for other.
Described increasing milk agent is Macrogol 2000, Macrogol 4000, Macrogol 6000, Triton X-100, NaCl, polysorbas20 or Tween 80.Increasing milk agent Main Function is the reaction rate that can increase antibody antigen.The preferred kind of increasing milk agent of the present invention is Macrogol 6000.
Described antiseptic is one or more in sodium azide, thimerosal, phenol.The preferred kind of the present invention is sodium azide.
Described stabilizing agent is one or more in bovine serum albumin(BSA), Macrogol 6000, sodium ethylene diamine tetracetate.
Described anti-human myoglobins antibody is the potpourri of monoclonal antibody or polyclonal antibody or monoclonal antibody and polyclonal antibody.
The described preparation method being marked with the nano rubber latex particulate of anti-human myoglobins antibody is: the carboxylic polystyrene nano rubber latex particulate selecting different-grain diameter, the carboxyl of microparticle surfaces is activated by activator 1-ethyl-3-(3-DimethylAminopropyl) carbodiimide hydrochloride (EDAC) and N-hydroxy thiosuccinimide (Sulfo-NHS), generate active ester, react with the primary amino radical on myoglobins monoclonal antibody or polyclonal antibody again, form covalent bond, complete coupling, make myoglobins monoclonal antibody or the polyclonal antibody nano rubber latex particle conjugate of different-grain diameter.The diameter of the carboxylic polystyrene nano rubber latex particulate of described different-grain diameter is commercially available prod at 50 ~ 300nm().Wherein, small particle diameter nano rubber latex grain conjugated monoclonal antibodies, can improve the range of linearity of detection; Large stone nano rubber latex particulate coupling polyclonal antibody, can improve detection sensitivity.
Principle of the present invention: the present invention utilizes the nano rubber latex particle conjugate being cross-linked antibody, after the antigen generation immune response in corresponding sample, form aggregated particle, under certain wavelength, the turbidity produced during by measuring aggregation and being formed, can measure the content of checking matter in sample.Further, in described the present invention, myoglobins in serum be cross-linked the anti-human myoglobins antibody on nano rubber latex particulate and be combined, generation antibody antigen reacts, nano rubber latex particulate is formed assemble, in 500nm wavelength place assaying reaction thing absorbance, the content of myoglobins in sample can be calculated by reference standard curve.
The present invention compared with prior art, has the following advantages:
1. the detection sensitivity of kit of the present invention can reach 0.2 μ g/L, and the range of linearity is 10 ~ 1000 μ g/L, and the two is all higher than sensitivity for analysis and the range of linearity of like product.That a kind of myoglobins that is highly sensitive, specificity good, accuracy is high, precision is good, the range of linearity is wide nano rubber latex particle type measures kit.
2. the particle diameter of nano rubber latex particulate involved in the present invention is at 50 ~ 300nm; The particulate of different-grain diameter is cross-linked with myoglobins monoclonal antibody or myoglobins polyclonal antibody respectively, the conjugate of multiple particle diameter can be formed; The conjugate of different-grain diameter, different antibodies is pressed different proportion mixing, the range of linearity that myoglobins measures kit can be widened, improve detection sensitivity.
Accompanying drawing explanation
Fig. 1 is the linear analysis chart of standard serum sample of 6 kinds of variable concentrations in the present invention;
Fig. 2 is the correlation analysis figure that myoglobins of the present invention measures kit.
Embodiment
Below by embodiment, the present invention is described in further detail, but the present invention is not only confined to following examples.
Embodiment 1
Myoglobins measures the preparation of kit
1. the preparation of reagent R1
In the phosphate buffer (pH7.4) of 20mmol/L, add final concentration is respectively 0.5% Macrogol 6000,0.1% polysorbas20,0.1% bovine serum albumin(BSA) and 0.1% sodium azide, fully mixes, with 0.22 μm of membrane filtration, and generate a reagent R1.
2. the preparation of reagent R2
Mouse-anti human muscle hemoglobin monoclonal antibody and the anti-human myoglobins polyclonal antibody of rabbit all entrust specialized company conventionally to obtain, and this antibody only reacts with human muscle hemoglobin, with other antigens without immunological cross-reaction, tires and can meet this test needs.
At 20mmol(pH6.1) add EDAC ︰ Sulfo-NHS ︰ nano rubber latex particulate (250nm or 60nm) in NES buffer solution, its mass ratio is 1 ︰ 0.6 ︰ 1, after mixing, room temperature rotary shaker activates 1 hour, the centrifugal 30min of 17000rpm, with 20mmol(pH6.1) NES buffer solution 3 times, then microballoon is suspended from 20mmol(pH6.1) NES damping fluid in, make microparticulate suspensions.
In 250nm microparticulate suspensions, add quality is the myoglobins polyclonal antibody that nano rubber latex particulate 1/4 is heavy, in 60nm microparticulate suspensions, add quality is the myoglobins monoclonal antibody that nano rubber latex particulate 1/4 is heavy, mix immediately, room temperature rotary shaker hatches 2 hours.Then add 1mol Tris-HCl damping fluid (pH7.4) respectively, make Tris-HCl buffer Final concentration be 0.1 mol, room temperature rotary shaker hatches 1 hour.Add the confining liquid of Tris-HCl damping fluid (pH7.4), 0.1% bovine serum albumin(BSA), 0.1% sodium azide and 0.03% Triton X-100 that final concentration is 20mmol more respectively, room temperature rotary shaker hatches 20 min.Centrifugal 30 min of 17000rpm under 2 ~ 10 DEG C of conditions, abandon supernatant, sediment respectively with containing final concentration be the phosphate buffer (pH7.4) of 30mmol, 0.1% sodium azide, 0.03% Triton X-100 cleansing solution resuspended, centrifugal again, washing like this 3 times, makes 250nm particulate and is cross-linked myoglobins polyclonal antibody conjugate and 60nm particulate is cross-linked myoglobins monoclonal antibody conjugates.With the resuspended conjugate respectively of phosphate buffer (pH7.4) damping fluid containing 0.1% bovine serum albumin(BSA), 0.1% sodium azide and 30mmol, its final concentration is made to be 0.25%(W/V), then with 1 ︰ 3 volume ratio mixing, generate a reagent R2.
Embodiment 2
Myoglobins measures the performance test of kit
Test analysis method: Two point end assay.Get reagent R1 150ul, add sample 25ul, add reagent R2 50 ul after 37 DEG C of 5min, start read point, reaction 5min, again read point, obtain absorbance difference (△ A).
Detecting instrument: Hitachi 7180 type automatic analyzer.
Determined wavelength: 500nm.
1. sensitivity test
Detect 20 water, record absorbance difference (△ A
water), calculating mean value (X
water) and standard deviation (SD
water); Detectable concentration is the sample 20 times of 100 μ g/L, record absorbance difference (△ A
sample), calculating mean value (X
sample) and standard deviation (SD
sample), test findings is as shown in table 1.With following formulae discovery sensitivity.
Sensitivity=100 × (X
water+ 3 × SD
water)/X
sample
Table 1
Sensitivity=100 × (0.691+3 × 0.0419)/398=0.2 μ g/L of reagent of the present invention
Result shows, and the sensitivity that myoglobins of the present invention measures kit is 0.2 μ g/L, is better than the sensitivity 1.59 μ g/L of the sensitivity 3 μ g/L of patent (CN 102628865 B), patent (CN 102565419 B).Result of study of the present invention reaches and improves the object that myoglobins measures kit assay sensitivity.
2. standard serum sample linear test
Be dissolved in the solution (the phosphate buffer pH7.4 of 0.9%NaCl, 0.1% bovine serum albumin(BSA), 0.1% sodium azide, 30mmol) of similar human serum matrix with recombined human myoglobins albumen, make 6 standard serum samples that concentration is 0.0 μ g/L, 100.0 μ g/L, 300.0 μ g/L, 600.0 μ g/L, 900.0 μ g/L, 1200.0 μ g/L.The sample of each variable concentrations measures kit with myoglobins of the present invention and detects 3 times, gets its mean value.Carry out correlation analysis to measured value, as shown in Figure 1, X-axis representation theory value (μ g/L) in figure, Y-axis represents measured value (μ g/L), r=0.9998, Y=0.970X ﹣ 4.121.Illustrate that kit of the present invention is that 0 ~ 1200 μ g/L scope internal linear relation is good in concentration, be better than the range of linearity 10 ~ 1000 μ g/L of the range of linearity 20 ~ 700 μ g/L of patent (CN 102565419 B), patent (CN 102628865 B).Result of study of the present invention reaches increases the object that RBP ELISA measures the kit range of linearity.
3. precision test
Detect each 20 times of the myoglobins human serum sample of high, medium and low 3 concentration with kit of the present invention, calculate withinrun precision; Detect each 20 times of the myoglobins human serum sample of same concentration with the kit of 3 lot numbers, calculate betweenrun precision.Test findings is as shown in table 2, and the withinrun precision (CV%) of kit of the present invention is respectively 2.89,0.82 and 0.71; Betweenrun precision (CV%) is 2.81.Illustrate that kit of the present invention has good precision.
Table 2
4. interference test
Get health check-up normal healthy human blood sample (without obvious haemolysis, jaundice, chyle), make pooled serum.Precision takes ascorbic acid, cholerythrin, haemoglobin and triglyceride, makes 4 groups of high concentration chaff interference solution.Precision measures high concentration chaff interference solution and pooled serum respectively, make the pooled serum sample (as shown in table 3) of disturbance substrate concentration, kit of the present invention for the serum sample prepared is measured respectively, each sample replication 3 times, get its mean value, measure average for 100% with the sample not adding chaff interference, calculate degree of disturbance.
Degree of disturbance=add chaff interference sample average/do not add chaff interference sample average × 100%
Degree of disturbance showing for acceptable result between 90% ~ 110%.
Table 3
Result show: when ascorbic acid concentrations be less than 300mg/L, bilirubin concentration is less than 400mg/L, hemoglobin concentration is less than 5g/L, triglyceride concentration is less than 10g/L time, the measurement result of each chaff interference to kit of the present invention has no significant effect.
5. correlation test
Kit of the present invention and contrast agents box (certain renowned company's similar-type products commercially available) is used to carry out correlation test and correlation analysis to 50 portions of normal human serums and 50 parts of abnormal human serums respectively.As shown in Figure 2, in figure, X-axis is contrast agents box measured value (μ g/L) to test findings, and Y-axis is kit measurement value of the present invention (μ g/L), correlation coefficient r=0.996 of two kits.Result shows: the detection data plot of kit of the present invention and contrast agents box is in linear relation, and two kits have significant correlativity.
The above is only preferred embodiment of the present invention, is not restriction the present invention being made to other form.In every case utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations, all do not depart from technical solution of the present invention content.According to any simple modification, equivalent variations and remodeling that technical spirit of the present invention is done above embodiment, still belong to the protection domain of technical solution of the present invention.
patent citation
| Authorize country and the patent No. | Announce or date of declaration | Patent name |
| CN 102565419 B | 2014.05.14 | Myoglobin assay kit |
| CN 102628865 B | 2014.07.16 | Detect the latex enhancing immune of myoglobin content than turbid kit |
non-patent citation
1. Zou Si Hunan. animal biochemistry (the 4th edition) Chinese agriculture publishing house, 2005.
2. Xiao Yu is gorgeous, Xing Yanfang, and rope is built quick. and myoglobins is determined at the clinical meaning in Diagnosis of AMI. labelling immunoassay and clinical, 2000,7 (2) ︰ 61 ~ 64.
3. Wu Ming. latex enhancing immune turbidimetry for Determination myoglobins. medical image and inspection, 2011,24 (5) ︰ 274 ~ 275.
4. Kang Kai, Kan Chengyou, etc. Polymer microsphere in biomedicine. chemical research and application, 2004,16 (2) ︰ 137 ~ 142.
5
.li Qing, Furong Li, Wu Xiongwen. the study and practice progress of nanometer technology in laboratory medicine, 2006,29 (5) ︰ 472 ~ 474.
Claims (10)
1. the myoglobins of a compound antibody measures kit, it is characterized in that: comprise reagent R1 and reagent R2, described reagent R1 be enhancing antibody antigentic specificity combine reactant liquor, component comprises: the damping fluid of 10 ~ 30mmol/L, 0.01% ~ 4%(w/v) increasing milk agent, 0.05% ~ 2%(w/v) stabilizing agent, 0.1%(w/v) antiseptic; Described reagent R2 is the damping fluid of the nano rubber latex particulate being combined with anti-human myoglobins antibody, and component comprises: 0.1% ~ 0.5%(w/v) be marked with nano rubber latex particulate, the 0.02% ~ 2%(w/v of anti-human myoglobins antibody) stabilizing agent, the damping fluid of 10 ~ 30mmol/L, 0.1%(w/v) antiseptic.
2. the myoglobins of compound antibody according to claim 1 measures kit, it is characterized in that: described damping fluid is one or more in TRIS buffer, the horizontal acid buffering solution of 2-morpholine second, phosphate buffer, borate buffer solution, glycine buffer, hydroxyethyl piperazine second thiosulfonic acid damping fluid and TRIS buffer; Described pH of cushioning fluid is 6 ~ 9, and described damping fluid can have the damping fluid of similar features for other.
3. the myoglobins of compound antibody according to claim 1 measures kit, it is characterized in that: described increasing milk agent is Macrogol 2000, Macrogol 4000, Macrogol 6000, Triton X-100, NaCl, polysorbas20 or Tween 80.
4. the myoglobins of compound antibody according to claim 1 measures kit, it is characterized in that: described antiseptic is one or more in sodium azide, thimerosal, phenol.
5. the myoglobins of compound antibody according to claim 1 measures kit, it is characterized in that: described stabilizing agent is one or more in bovine serum albumin(BSA), Macrogol 6000, sodium ethylene diamine tetracetate.
6. the type myoglobins of compound antibody according to claim 1 measures kit, it is characterized in that: described anti-human myoglobins antibody is the potpourri of monoclonal antibody or polyclonal antibody or monoclonal antibody and polyclonal antibody.
7. the myoglobins of compound antibody according to claim 1 measures kit, it is characterized in that: described nano rubber latex particulate is the polystyrene latex microparticles modified by chemical group, the preferred carboxyl of described chemical group.
8. the myoglobins of the compound antibody according to claim 1 or 6 ~ 7 measures kit, it is characterized in that: described in be marked with the nano rubber latex particulate of anti-human myoglobins antibody preparation method be: the pipe/polyhenylethylene nano present latex particulate selecting different-grain diameter, under the effect of activator, amino condensation on carboxyl on ps particle and antibody forms conjugate, and the diameter of the pipe/polyhenylethylene nano present latex particulate of described different-grain diameter is at 50 ~ 300nm.
9. the myoglobins of the compound antibody according to claim 1 or 8 measures kit, it is characterized in that: described reagent R2 is 0.1% ~ 0.5%(w/v) cocktail buffer of polyclonal antibody conjugate and monoclonal antibody conjugates, the mass ratio of described monoclonal antibody conjugates and polyclonal antibody conjugate is 1:1 ~ 5:1.
10. the myoglobins of the compound antibody according to any one of claim 1 ~ 9 measures kit, it is characterized in that: for measuring the content of myoglobins in human serum.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105911298A (en) * | 2016-05-27 | 2016-08-31 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining myoglobin |
| CN107490677A (en) * | 2017-07-21 | 2017-12-19 | 王贤俊 | The cross-linking composition liquid and its cross-linking method of a kind of carboxylated latex microballoon and glycosylated hemoglobin antibody |
| CN107515296A (en) * | 2017-07-21 | 2017-12-26 | 王贤俊 | A kind of coupling method of myoglobins antibody latex microballoon |
| CN107677832A (en) * | 2017-09-20 | 2018-02-09 | 北京众驰伟业科技发展有限公司 | A kind of vWF ELISA detection reagent and its preparation method and application |
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| CN111693719A (en) * | 2019-03-11 | 2020-09-22 | 程明 | Myoglobin determination kit and determination method thereof |
| CN111781372A (en) * | 2020-06-29 | 2020-10-16 | 安徽大千生物工程有限公司 | Alpha 1-AT immunoturbidimetry detection kit based on mixed antibody and preparation and use methods thereof |
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| CN102628865A (en) * | 2011-12-30 | 2012-08-08 | 北京九强生物技术股份有限公司 | Latex enhanced immunoturbidimetry kit for detection of myoglobin content |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105911298A (en) * | 2016-05-27 | 2016-08-31 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining myoglobin |
| CN107490677A (en) * | 2017-07-21 | 2017-12-19 | 王贤俊 | The cross-linking composition liquid and its cross-linking method of a kind of carboxylated latex microballoon and glycosylated hemoglobin antibody |
| CN107515296A (en) * | 2017-07-21 | 2017-12-26 | 王贤俊 | A kind of coupling method of myoglobins antibody latex microballoon |
| CN107515296B (en) * | 2017-07-21 | 2019-02-26 | 王贤俊 | A kind of coupling method of myoglobins antibody latex microballoon |
| CN107677832A (en) * | 2017-09-20 | 2018-02-09 | 北京众驰伟业科技发展有限公司 | A kind of vWF ELISA detection reagent and its preparation method and application |
| CN108761089A (en) * | 2018-06-29 | 2018-11-06 | 迈克生物股份有限公司 | Preparation method for the reagent for detecting β2-microglobulin |
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