CN104512962B - Method for enhancing anaerobic ammoxidation effect of deposit by using water treatment plant waste mud - Google Patents
Method for enhancing anaerobic ammoxidation effect of deposit by using water treatment plant waste mud Download PDFInfo
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- CN104512962B CN104512962B CN201310456254.XA CN201310456254A CN104512962B CN 104512962 B CN104512962 B CN 104512962B CN 201310456254 A CN201310456254 A CN 201310456254A CN 104512962 B CN104512962 B CN 104512962B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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Abstract
The invention provides a method for enhancing the anaerobic ammoxidation effect of a deposit by using water treatment plant waste mud (WTRs) as a carrier, and belongs to the technical field of water treatment. The method is characterized in that the deposit and the WTRs are enriched through a closed anaerobic cylindrical device, and a deposit-WTRs mixture with the wet-to-dry weight ratio of 10:1 is added to synthetic water rich in ammonia nitrogen and is enriched for a period of time to obtain a deposit containing high activity and high abundance anaerobic ammoxidation bacteria. The purposes comprise the stimulation of the in situ environment of the deposit in eutrophic water and the provision of much interception indication for the anaerobic ammoxidation bacteria by using the large specific surface area of the WTRs as a carrier. The WTRs contain a large amount of iron, can weaken the inhibition effect of P, heavy metals and other substances in the deposit on the anaerobic ammoxidation effect, and provide suitable living environment for the anaerobic ammoxidation bacteria. The method ahs the advantages of low cost of the used materials, environmental protection, simple technological operation, solving of the treatment problem of the WTRs, realization of the effective control of endogenous release of the eutrophic water, and 'using waste to treat waste' purpose realization facilitation.
Description
Technical field
The invention belongs to water treatment field, or environmental project, or environmental microorganism.It is specially a kind of discarded using water treatment plant
The method that mud strengthens the effect of deposit Anammox.
Background technology
The water pollution situation of China is serious, and the water ecological environment such as most rivers, lake, wetland is subject to the prestige of eutrophication
The side of body, wherein, especially with one of problem of the excessively a height of most serious of ammonia-nitrogen content.Ammonia and nitrogen pollution source includes external source pollute enter with endogenous
Pollution release.External source pollution generally comprises the discharge of water environment periphery point source dough-making powder source pollution.With water environment protection consciousness and
The enhancing of regulation, external source pollution is effectively controlled, and ripe more than the processing method of external source pollution and technique.Endogenous dirt
Dye is administered becomes the emphasis controlling body eutrophication and difficult point.The biochemistry mistake of the participation denitrogenation having been found that in natural water
Journey, most importantly Denitrification and Anammox act on.Wherein, Anammox acts on due to not producing greenhouse gases
n2O, can remove denitrification pollutant simultaneously, and obtains increasing concern and attention.Further, since deposit with upper
Cover and there is obvious oxidoreduction potential gradient between water or dissolved oxygen concentration is poor, can provide for anaerobic ammonium oxidizing bacteria good
Habitat.Therefore, improve the habitat of deposit surface anaerobic ammonium oxidizing bacteria, strengthen Anammox effect, there is important answering
Use meaning.
The key constraints of Anammox effect are the doubling time length of antibacterial and habitat are had high demands.Anaerobism
The requirement to dissolved oxygen and oxidation-reduction potential for the ammonia oxidizing bacteria is higher, and under anaerobism and anoxia condition, anaerobic ammonium oxidizing bacteria is lived
Property strong, and under micro- aerobic condition, its activity is subject to reversible inhibition, but under long-term aerobic condition, its activity can produce not
Reversible forfeiture.Anammox effect is subject to the inhibitory action of phosphate, heavy metal and hydrogen sulfide to varying degrees, and fe
(iii) in the range of finite concentration, Anammox effect can be strengthened.Impact Anammox being acted on according to these factors
Feature, at present, many researcheres are enriched with anaerobic ammonia oxidizing bacteria from environmental sample or mud, build bioreactor, for dirt
Water process.There is researcher to utilize admixture to provide antibacterial suitable growth environment, but be using fixing material and anaerobism ammonia oxygen mostly
The method changing mud mixing is fixed, and puts into reactor using the activation culture front needing 1-2 week.As: application is (specially
Profit) number cn201110029355.x discloses～kind using activated carbon fixed effect strengthened anaerobic ammoxidation microbial activity side
Method, fixing material activity charcoal first passes through a series of pre-treatments, such as acid-alkali treatment, go dust, soak with hydrochloric acid, clear water to rinse, hydrogen
Sodium oxide immersion, tap water distilled water flushing, normal saline are soaked and are dried, and are to detest using its three-dimensional loose shape loose structure
Anaerobic ammonium oxidation microorganism provides microenvironment.Similar also has Application Number (patent) cn201110029658.1, it discloses one kind
The method embedding strengthened anaerobic ammoxidation microbial activity using polyvinyl alcohol-sodium alginate-activated carbon.Application Number (patent)
201110266476.6 publication No. cn102336472a discloses a kind of electricity strengthens denitrification of anaerobic ammonium oxidation method, utilize
Iron-graphite electrode extra electric field, the release of regulation and control ferrum, provide enough ferrous substrates for anaerobic ammonia oxidizing bacteria, promote Anammox
Bacteria growing.Researcher is had to carry out the side of Enrichment of bacteria in anaerobic biological reactor system using inoculation anaerobic ammonium oxidizing bacteria
Method, majority is for the purpose of providing more long residence time for microorganism.As: Application Number (patent) cn200410042793 discloses
A kind of method that utilization mixing sludge cultivates anaerobic ammonium oxidation granular sludge.Application Number (patent) cn201010153704.4 is open
A kind of the flow reactor upwards (uasb) in, using the mixing inactivating methane phase granule sludge and Anammox floc sludge
Mud, the method realizing anaerobic ammonium oxidation granulation.Application Number (patent) cn200710164839.9 discloses one kind using upper
The device of up-flow three-phase separator device efficiently concentrating anaerobic ammonia oxidizing bacteria.Application Number (patent) cn200910155264.3 is (real
With new patent application number 200920202098.3 and Authorization Notice No. cn201620166u) disclose a kind of simulating natural environment
Soil anaerobic ammoxidation enrichment system.
Wtrs is a kind of safe garbage in water treatment plant, rich in fe/al.These fe/al are mainly with amorphous state
Exist, make wtrs have larger specific surface area, and have preferable absorbability to various materials.Moreover, wtrs is to heavy
In long-pending thing, active p and metal are respectively provided with preferable crystallized ability.Based on this it is believed that mix wtrs with deposit, have
Help improve habitat in deposit for the anaerobic ammonium oxidizing bacteria, anaerobic ammonium oxidizing bacteria in Sediment Water Interface can be eliminated
Retardation of growth, and then in enhanced deposition thing Anammox effect.
Content of the invention
The invention provides the enhancing eutrophic water deposit with water treatment plant's waste sludge (wtrs) as carrier for the one kind is detested
The method of anaerobic ammonium oxidation effect.
The present invention tests enrichment deposit and wtrs by column, then, adds ammonia nitrogen rich in deposit-wtrs mixture
Rich synthetic water is enriched with.Purpose is the original position microenvironment of simulation fresh water environment deposit eutrophication.Concrete technical scheme
As follows:
1) fresh wtrs is dried naturally and sieve；
2) according to a certain percentage, by step 1) in wtrs uniformly mix with deposit；
3) by step 2) in mixture be filled into column enriching apparatus；
4) in the way of bottom-up, periodically to step 3) in device supply cultivated rich in the nutritional solution of ammonia nitrogen.
Advantages of the present invention and having the beneficial effect that:
1) raw material of the present invention is water treatment plant's waste sludge, belongs to the safe disposal thing of water treatment plant, has the advantages that easy acquisition；
2) operating process of the present invention is simple；
3) present invention utilizes the own characteristic of water treatment plant's waste sludge, improves anaerobic ammonium oxidizing bacteria habitat, enhances deposition
Anammox effect in thing；
4) present invention utilizes water treatment plant's waste sludge, is advantageously implemented the doulbe-sides' victory effect of " treatment of wastes with processes of wastes against one another ".
Brief description
Accompanying drawing described herein is used for providing a further understanding of the present invention, constitutes the part of the application.This
Bright schematic description and description is used for explaining the present invention, does not constitute the improper restriction to the present invention.In the accompanying drawings:
Fig. 1 be the embodiment of the present invention in cylindrical reactor (add wtrs enrichment after deposit) water inlet and water outlet molten
Solution oxygen content change.
Fig. 2 is the changes of contents of active phosphorus and as (iii) in deposit before and after the enrichment in the embodiment of the present invention.
Fig. 3 is the fluorescence in situ hybridization before and after anaerobic ammonium oxidizing bacteria enrichment in the deposit in the embodiment of the present invention
(fish) figure.
Fig. 4 is the Plantago fengdouensis before and after anaerobic ammonium oxidizing bacteria enrichment in the deposit in the embodiment of the present invention.
Fig. 5 is the activity change before and after anaerobic ammonium oxidizing bacteria enrichment in the deposit in the embodiment of the present invention.
Specific embodiment
Below with reference to the accompanying drawings and in conjunction with the embodiments, to describe the present invention in detail.
Example one: with the waste sludge (wtrs) of water treatment plant of Beijing as raw material, in this waste sludge, the content of ferrum and aluminum is divided
Wei not 150 and 100mg g-1.Water treatment plant's waste sludge, after naturally drying, crosses 100 mesh sieves.Take North China's eutrophic lake
Deposit 100g, wtrs above-mentioned with 5g uniformly mixes, and the latter's dosage is the 10% of the former dry weight；Mixture is added to a post
(internal diameter: 50mm in son；Pillar height: 100mm), the bottom of pillar and top are all lined with nylon mesh, prevent deposit from flowing out.Additionally,
In second pillar, the deposit not adding wtrs is enriched with, and with this as matched group.Only add in 3rd pillar
Wtrs is enriched with, and with this as blank.For ensureing that system is in anaerobic state, remove outside water inlet and outlet,
Whole pillar is all in sealing state, and use exposed n2Deionized water configuration water inlet.Intake by the way of from the bottom up,
The flow velocity of water inlet is 200mlday-1, ph value is 8, and initial po4 3-、nh4 +、no2 -And no3 -Concentration respectively 1.5mm,
3.6mm, 0.6mm and 1.0mm, measured nh in water outlet every 3 days4 +、no2 -And no3 -Concentration.Cylindrical reactor is enriched with through 50-80d
Reach the purpose of Anammox effect in enhanced deposition thing.
The change of anaerobism microenvironment: dissolved oxygen monitoring result shows (Fig. 1), by the way of nitrogen is exposed using batch (-type),
In water inlet water body, dissolved oxygen content maintains relatively low level all the time, is in 0.5mg l-1Below.And cylindrical reactor (adds
Wtrs enrichment after deposit) water outlet only initial operating stage occur be higher than 0.2mg l-1Situation, after operation phase dissolved oxygen contain
Amount is almost nil.In deposit, the analysis result of active phosphorus and as (iii) shows (Fig. 2) after enrichment, adds the deposition of wtrs
In thing, the content of active phosphorus and as (iii) is significantly lower than not plus wtrs is enriched with the content in deposit, and is less than in-situ deposition thing
In content.Therefore, wtrs contributes to improving deposit microenvironment so as to the growth of more suitable anaerobic ammonium oxidizing bacteria.
The determination of anaerobic ammonium oxidizing bacteria distribution: fluorescence in-situ hybridization method (fish) result shows (Fig. 3), real in enrichment
In deposit before and after testing, the existence form of anaerobic ammonia oxidizing bacteria is different.In in-situ deposition thing anaerobic ammonium oxidizing bacteria mainly with
Presented in dispersion, and in deposit after being enriched with, anaerobic ammonium oxidizing bacteria is existed in the way of more gathering, and this poly-
Become apparent from group's deposit after being enriched with containing wtrs for the form.
Example two: same with the waste sludge (wtrs) of water treatment plant of Beijing as raw material, this waste sludge after naturally drying,
Cross 100 mesh sieves.North China high ammonia and nitrogen pollution river deposit 80g, wtrs above-mentioned with 5g is taken uniformly to mix, the latter's dosage
For the former dry weight 10%；Mixture is added to (internal diameter: 50mm in a pillar；Pillar height: 100mm), the bottom of pillar and top
Portion is all lined with nylon mesh, prevents deposit from flowing out.Additionally, being enriched with to the deposit not adding wtrs in second pillar,
And with this as matched group.For ensureing that system is in anaerobic state, remove outside water inlet and outlet, whole pillar is all in close
Envelope state, and use exposed n2Deionized water configuration water inlet.Intake by the way of from the bottom up, the flow velocity of water inlet is
200ml day-1, ph value is 8, and initial nh4 +、no2 -And no3 -Concentration was respectively 10.0mm, 3.0mm and 5.0mm, every 3 days
Measure nh in water outlet4 +、no2 -And no3 -Concentration.Cylindrical reactor reaches Anammox in enhanced deposition thing through 90-150d enrichment
The purpose of effect.
The determination of anaerobic ammonium oxidizing bacteria abundance: quantitative pcr result shows (Fig. 4), Anammox in primary deposit thing
The abundance of antibacterial is 5.9 × 107copiesg-1, after enrichment experiment, the abundance of anaerobic ammonia oxidizing bacteria is in the deposition without wtrs
8.9 × 10 have been brought up in thing7copies g-1, the deposit containing wtrs brings up to 1.4 × 108copies g-1.Cause
This, wtrs improves deposit microenvironment, thus improving the activity of anaerobic ammonium oxidizing bacteria and abundance in deposit.
The determination of anaerobic ammonium oxidizing bacteria activity:15N isotopic tracing is test result indicate that (Fig. 5), the enrichment containing wtrs
In deposit, Anammox action activity is higher than the deposit without wtrs afterwards, and this species diversity is especially embodied in isotope and shows
After the 48h of track experiment.Test result shows, after enrichment experiment, anaerobic ammoxidation activity is by the 1.9nmol n of primary deposit thing
g-1h-1, it is respectively increased 6.1nmol n g-1h-1(not plus after wtrs enrichment) and 9.2nmol n g-1h-1(plus wtrs enrichment
Afterwards).
The detailed description it is emphasized that although above-described embodiment is contrasted to the present invention, these explanations are only
Illustrative to the present invention, rather than the restriction to invention, any without departing from the innovation and creation in true spirit, all
Fall within rights protection scope of the present invention.
Claims (3)
1. a kind of method of utilization water treatment plant waste sludge enhanced deposition thing Anammox effect is it is characterised in that particular technique
Scheme is as follows:
(1) fresh water treatment plant's waste sludge is naturally dried and sieved；Fe/al material, fe is contained in described water treatment plant waste sludge
Content is 150mg/g, and al content is 100mg/g；
(2) according to deposit and water treatment plant's waste sludge dry weight than the ratio for 10: 1, by the water treatment plant's waste sludge in step (1)
Uniformly mix with deposit；
(3) mixture in step (2) is filled into column enriching apparatus；
(4) in the way of bottom-up, periodically cultivated to the nutritional solution rich in ammonia nitrogen for the device supply in step (3).
2. the method for water treatment plant according to claim 1 waste sludge enhanced deposition thing Anammox effect, its feature exists
Contain anaerobic ammonium oxidizing bacteria in deposit, in environment water, there is a large amount of ammonia nitrogens, and the presence with nitrogen oxides.
3. the method for water treatment plant according to claim 1 waste sludge enhanced deposition thing Anammox effect, its feature exists
In enriching apparatus sealing simultaneously anaerobic.
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| CN105084552A (en) * | 2015-07-31 | 2015-11-25 | 北京师范大学 | Method for enhancing aerobiotic ammonia oxidizing bacterium gathering through N-acylated homoserine lactones |
| CN108499514A (en) * | 2018-04-10 | 2018-09-07 | 中国科学院南京地理与湖泊研究所 | A kind of phosphorus adsorbent and preparation method thereof based on water supply plant iron aluminium mud |
| CN113493245B (en) * | 2021-05-06 | 2022-11-22 | 北京工业大学 | Method for culturing anaerobic ammonia oxidation granular sludge with vivianite core |
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| CN1807594A (en) * | 2006-02-14 | 2006-07-26 | 浙江大学 | Separation and authentication method for denitrifying bacteria with anaerobic ammoxidation activity |
| JP2009142810A (en) * | 2007-11-21 | 2009-07-02 | Kato Construction Co Ltd | Water purifying treatment method |
| CN101525179B (en) * | 2009-04-20 | 2011-01-26 | 广东省微生物研究所 | Method for anaerobic ammonia oxidation treatment of low-concentration ammonia nitrogen wastewater |
| CN102190371B (en) * | 2010-03-18 | 2013-01-30 | 华东师范大学 | Breeding method for anaerobic ammonium oxidation granular sludge |
| CN102344197B (en) * | 2011-10-18 | 2012-09-05 | 浙江大学 | Method for rapidly starting anaerobic ammonium oxidation reactor |
| CN103087910B (en) * | 2013-01-22 | 2014-08-06 | 桂林电子科技大学 | Device and method for fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria |
| CN103193315B (en) * | 2013-04-12 | 2014-05-07 | 北京工业大学 | Method for preparing bioactive filler through immobilization of anaerobic ammonia oxidizing bacterium |
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