CN104509679B - A kind of method that utilization defatted soybean meal produces soy glycoproteins - Google Patents
A kind of method that utilization defatted soybean meal produces soy glycoproteins Download PDFInfo
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- CN104509679B CN104509679B CN201410678164.XA CN201410678164A CN104509679B CN 104509679 B CN104509679 B CN 104509679B CN 201410678164 A CN201410678164 A CN 201410678164A CN 104509679 B CN104509679 B CN 104509679B
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Abstract
The present invention relates to a kind of method that utilization defatted soybean meal produces soy glycoproteins, it is to choose defatted soybean meal for raw material, is soaked with water, and sodium hydroxide is added after heating, defoaming treatment and adjusts pH value to alkalescent, cooling addition protease hydrolyzed obtains enzymolysis liquid；Go out and atlapulgite, flocculant are added after enzyme, staticly settle；Supernatant is taken, soy glycoproteins clear liquid is obtained；Soy glycoproteins clear liquid adds activated carbon and carries out the de- clarification of carbon, and filtering obtains soy glycoproteins filtered fluid；Soy glycoproteins filtered fluid is concentrated, spray drying, obtains soy glycoproteins.Its enzymolysis process mild condition, easy control of reaction；Its flocculation purification technique sloughs the impurity in solution, and its carbon is de- to carry out decolouring clarification to clear liquid, improve thing purity.Present invention process is advanced, and rationally, operation is controllable, is suitable for large-scale production for process.
Description
Technical field
It is especially a kind of to be produced using defatted soybean meal the present invention relates to glycoprotein is produced from oil cake or other oil-containing seeds
The method of soy glycoproteins.
Background technology
China has the soybean up to more than one thousand years to eat history, and soybean protein aboundresources at home, plantation is extensive.Degreasing beans
The dregs of rice refer to soybean through cleaning, quenched, rupture, peeling, tabletting, and under given conditions by organic solvent-normal hexane ex hoc genus anne carbon
The powder being made after hydrogen compound degreasing, then toasted, crushing.Soybean protein content is up to 43~48% in defatted soybean meal, removes
Sulfur-containing amino acid shortcoming is outer, and other amino acid have fabulous balance, especially lysine, and its content is up to 2.5~2.8%, is
Replace one of best vegetable protein of vegetable protein.At present, for various reasons, defatted soybean meal deep processing and utilization degree ratio is caused
It is relatively low, it is mainly used in feed, brewages etc. in the relatively low purposes of added value.
In recent years research shows that glycoprotein, with very strong inhibitory action, causes people to glycoprotein to the growth of tumour
Extensive concern.
The content of the invention
In order to overcome the shortcomings of that defatted soybean meal is low using added value in the prior art, the present invention provide a kind of technique it is advanced,
The method that process is reasonable, a kind of utilization defatted soybean meal of easy controlled operation produces soy glycoproteins, the soybean produced using this method
Glycoprotein improves the added value of defatted soybean meal.
The technical solution adopted for the present invention to solve the technical problems is：One kind produces soy glycoproteins using defatted soybean meal
Method, it is characterised in that：By following process steps：
(1), the selection and processing of raw material
Selection defatted soybean meal is raw material, standby；
(2), immersion, the processing of acid adjustment basicity
The defatted soybean meal chosen in step (1) is sent into enzymatic vessel, the deionized water of 3~8 times of defatted soybean meal weight is added,
Immersion 1~2 hour, is warming up to 70~80 DEG C, adds defoamer, stirs；Then sodium hydroxide regulation is added while stirring
PH is 8.5~9.5, is incubated 10~60 minutes；Wherein, the consumption of described defoamer is the 0.1~0.3% of dregs of beans weight；
(3), digest
Defatted soybean meal after immersion, the processing of acid adjustment basicity in step (2) is reduced into temperature to 40~50 DEG C, degreasing beans are added
The protease of dregs of rice weight 2~4%, constant temperature is digested 3~5 hours, and defatted soybean meal enzymolysis liquid is made；
(4), go out enzyme
Obtained defatted soybean meal enzymolysis liquid in step (3) is heated to 80~85 DEG C, 10~45 minutes is incubated, is gone out
Ferment treatment, obtains the defatted soybean meal enzymolysis liquid of enzyme-deactivating；
(5), flocculation purification
The defatted soybean meal enzymolysis liquid of the enzyme-deactivating of gained in step (4) is transferred to flocculation tank, atlapulgite, stirring is added
Uniformly, flocculant is then added while stirring, is staticly settled 30~50 minutes, remaining beans will be digested in defatted soybean meal enzymolysis liquid
The dregs of rice are flocculated by flocculant, atlapulgite adsorption precipitation to tank bottom；The clear liquid on flocculation tank upper strata is taken, soy glycoproteins clear liquid is obtained；Its
In, the consumption of described atlapulgite is the 5~8% of defatted soybean meal weight, and the consumption of described flocculant is defatted soybean meal weight
3~4%；
(6), carbon is de-, filter
The soy glycoproteins clear liquid of gained in step (5) is transferred to carbon and takes off tank, the activated carbon of its weight 3~6% is added,
80~85 DEG C are warming up to, stirring insulation carries out the de- clarification of carbon for 30~60 minutes, filtering obtains soy glycoproteins filtered fluid；
(7), concentrate, be spray-dried
Soy glycoproteins filtered fluid obtained by step (6) is concentrated, is re-fed into spray dryer and is sprayed
Mist is dried, and obtains soy glycoproteins.
Described protease is one kind or one in compound protease, flavor protease, papain, alkali protease
More than kind.
Described atlapulgite is high-efficient activated clay.
Described sodium hydroxide is food sodium hydroxide.
Described defoamer is commercially available bean product defoamer.
Described flocculant is composite flocculation agent I, the composite flocculation agent II that Weifang clear water Chemical Co., Ltd. produces,
Composite flocculation agent I and composite flocculation agent II in proportion 1:1 is used as flocculant, and the molten of concentration 15~25% is made respectively
Liquid.
Filter-cloth filtering or centrifugal filtration are filtered into after the de- clarification of described collagen clear liquid carbon.
Described centrifugal filtration is to control the rotating speed of centrifuge for 4000rpm~20000rpm, and centrifugation time is 5~15 points
Clock.
The condition of work of described spray drying is：Inlet temperature is 200~230 DEG C, and outlet temperature is 82~85 DEG C, mist
Change disk rotating speed and be located in 48Hz, tower negative pressure in 8~10Pa.
The present invention is to choose defatted soybean meal for raw material, is soaked with water, and sodium hydroxide is added after heating, defoaming treatment and is adjusted
PH value is saved to alkalescent, then cooling adds protease and carries out enzymatic hydrolysis, obtains defatted soybean meal enzymolysis liquid；Go out and activity is added after enzyme
Carclazyte, flocculant, are staticly settled；Supernatant is taken, soy glycoproteins clear liquid is obtained；Soy glycoproteins clear liquid adds activated carbon and carries out carbon
De- clarification, filtering obtains soy glycoproteins filtered fluid；Soy glycoproteins filtered fluid is concentrated, spray drying, obtains soy glycoproteins.
The present invention after defatted soybean meal immersion, regulation pH value using bio-enzyme degradation technology to being handled, its enzymolysis process mild condition,
Reaction is easily controlled, and the protein enriched in defatted soybean meal is converted into protein peptides；Enzymolysis in its defatted soybean meal enzymolysis liquid is remained
Remaining dregs of beans is flocculated by flocculant, atlapulgite adsorption-flocculation precipitation, preferably sloughs the impurity in solution；Its soy glycoproteins is clear
Liquid carries out decolouring clarification using activated carbon, improves the purity of product.Present invention process is advanced, and rationally, operation is controllable for process, fits
Together in large-scale production, the soy glycoproteins purity produced is high, improves the added value of defatted soybean meal.
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of method that utilization defatted soybean meal produces soy glycoproteins, by following process steps：
(1), the selection and processing of raw material
It is raw material to choose the remaining defatted soybean meal of selection soybean grease factory squeezing grease, standby；
Compound protease is chosen, it is standby；
Commercially available qualified food sodium hydroxide, high-efficient activated clay are chosen, it is standby；
The compound bean product defoamer that Zhengzhou high aim Food Co., Ltd produces and sells is chosen, it is standby；
Choose composite flocculation agent I, the composite flocculation agent II, composite flocculation agent of Weifang clear water Chemical Co., Ltd. production
No. I with composite flocculation agent II in proportion 1:1 is used as flocculant, and the solution of concentration 20% is made respectively, standby；
(2), immersion, the processing of acid adjustment basicity
Defatted soybean meal in step (1) is sent into enzymatic vessel, the deionized water for adding 5 times is soaked 1.5 hours, is warming up to 75
DEG C, compound bean product defoamer selected in step (1) is added, is stirred, then adds in step (1) and selects while stirring
The food sodium hydroxide regulation PH taken is 9；Insulation 30 minutes；Wherein, the consumption for being combined bean product defoamer is dregs of beans weight
0.2%；
(3), digest
By the defatted soybean meal after immersion, the processing of acid adjustment basicity in step (2), reduction temperature adds step to 45 DEG C（1）In
The compound protease of selection, the compound protease is commercially available Novi's letter compound protease, and constant temperature is digested 4 hours, and degreasing is made
Dregs of beans enzymolysis liquid；Wherein, compound protease with 3% that enzyme amount is defatted soybean meal weight；
(4), go out enzyme
Obtained defatted soybean meal enzymolysis liquid in step (3) is heated to 82 DEG C, 30 minutes is incubated, carries out destroy the enzyme treatment, obtain
The defatted soybean meal enzymolysis liquid of enzyme-deactivating；
(5), flocculation purification
The defatted soybean meal enzymolysis liquid of the enzyme-deactivating of gained in step (4) is transferred to flocculation tank, added selected in step (1)
The high-efficient activated clay taken, is stirred, and the flocculant produced in step (1) is then added while stirring, 40 are staticly settled
Minute, will be digested in defatted soybean meal enzymolysis liquid remaining dregs of beans flocculated by flocculant, atlapulgite adsorption precipitation to tank bottom；Take wadding
The clear liquid on solidifying tank upper strata, obtains soy glycoproteins clear liquid；Wherein, the consumption of high-efficient activated clay is the 7% of defatted soybean meal weight, wadding
The consumption of solidifying agent is the 3.5% of defatted soybean meal weight；
(6), carbon is de-, filter
The soy glycoproteins clear liquid of gained in step (5) is transferred to carbon and takes off tank, the activated carbon of its weight 4.5% is added, risen
Temperature is to 83 DEG C, stirring insulation 45 minutes, carries out the de- clarification of carbon, and filter-cloth filtering obtains soy glycoproteins filtered fluid；
(7), concentrate, be spray-dried
Soy glycoproteins filtered fluid obtained by step (6) is concentrated, is re-fed into spray dryer and is sprayed
Mist is dried, and obtains soy glycoproteins；The condition of work of spray drying is：Inlet temperature is 215 DEG C, and outlet temperature is 83 DEG C, atomization
Disk rotating speed is located in 48Hz, tower negative pressure in 9Pa.
The utilization defatted soybean meal that the present embodiment is provided is produced in the method for soy glycoproteins, to defatted soybean meal immersion, is adjusted
Save using biology enzyme progress enzymolysis processing after pH value, its enzymatic hydrolysis condition is gentle, and reaction is easily controlled, by what is enriched in defatted soybean meal
Protein is converted into protein peptides；And the remaining dregs of beans of enzymolysis in defatted soybean meal enzymolysis liquid is flocculated by flocculant, atlapulgite is inhaled
Attached flocculation sediment, preferably sloughs the impurity in solution；Clear liquid carries out decolouring clarification using activated carbon, improves the purity of product.
Its preparation method technique is advanced, and rationally, operation is controllable, is suitable for large-scale production for process.
Embodiment 2
A kind of method that utilization defatted soybean meal produces soy glycoproteins, by following process steps：
(1), the selection and processing of raw material
It is raw material to choose the remaining defatted soybean meal of selection soybean grease factory squeezing grease, standby；
Papain, alkali protease are chosen, it is standby；
Commercially available qualified food sodium hydroxide, high-efficient activated clay are chosen, it is standby；
The compound bean product defoamer of Zhengzhou high aim Food Co., Ltd production is chosen, it is standby；
Choose composite flocculation agent I, the composite flocculation agent II, composite flocculation agent of Weifang clear water Chemical Co., Ltd. production
No. I with composite flocculation agent II in proportion 1:1 is used as flocculant, and the solution of concentration 15% is made respectively, standby；
(2), immersion, the processing of acid adjustment basicity
Defatted soybean meal in step (1) is sent into enzymatic vessel, the deionized water for adding 3 times is soaked 2 hours, is warming up to 70 DEG C,
The compound bean product defoamer chosen in step (1) is added, is stirred, then adds what is chosen in step (1) while stirring
Food sodium hydroxide regulation PH is 9.5；Insulation 10 minutes；The consumption of wherein described defoamer is the 0.3% of dregs of beans weight；
(3), digest
By the defatted soybean meal after immersion, the processing of acid adjustment basicity in step (2), reduction temperature adds step to 40 DEG C（1）In
Papain, the alkali protease of selection, constant temperature are digested 5 hours, and defatted soybean meal enzymolysis liquid is made；Wherein, papain
With enzyme amount is defatted soybean meal weight 1%, alkali protease with 1% that enzyme amount is defatted soybean meal weight；
(4), go out enzyme
Obtained defatted soybean meal enzymolysis liquid in step (3) is heated to 85 DEG C, 10 minutes is incubated, carries out destroy the enzyme treatment, obtain
The defatted soybean meal enzymolysis liquid of enzyme-deactivating；
(5), flocculation purification
The defatted soybean meal enzymolysis liquid of the enzyme-deactivating of gained in step (4) is transferred to flocculation tank, added selected in step (1)
The high-efficient activated clay taken, is stirred, and the flocculant produced in step (1) is then added while stirring, 50 are staticly settled
Minute, will be digested in defatted soybean meal enzymolysis liquid remaining dregs of beans flocculated by flocculant, atlapulgite adsorption precipitation to tank bottom；Take wadding
The clear liquid on solidifying tank upper strata, obtains soy glycoproteins clear liquid；Wherein, the consumption of high-efficient activated clay is the 5% of defatted soybean meal weight, wadding
The consumption of solidifying agent is the 4% of defatted soybean meal weight；
(6), carbon is de-, filter
The soy glycoproteins clear liquid of gained in step (5) is transferred to carbon and takes off tank, the activated carbon of its weight 6% is added, heated up
To 80 DEG C, stirring insulation 60 minutes carries out the de- clarification of carbon, and feeding filter centrifugal controls rotating speed to be 4000rpm, filters 15 points
Clock, obtains soy glycoproteins filtered fluid；
(7), concentrate, be spray-dried
Soy glycoproteins filtered fluid obtained by step (6) is concentrated, is re-fed into spray dryer and is sprayed
Mist is dried, and obtains soy glycoproteins；The condition of work of spray drying is：Inlet temperature is 200 DEG C, and outlet temperature is 85 DEG C, atomization
Disk rotating speed is located in 48Hz, tower negative pressure in 8Pa.
The method that the utilization defatted soybean meal that the present embodiment is provided produces soy glycoproteins, present invention process is advanced, process
Rationally, treatment conditions are gentle, and operation is controllable, is suitable for large-scale production.
Embodiment 3
A kind of method that utilization defatted soybean meal produces soy glycoproteins, it is characterised in that：By following process steps：
(1), the selection and processing of raw material
It is raw material to choose the remaining defatted soybean meal of selection soybean grease factory squeezing grease, standby；
Flavor protease, papain, alkali protease are chosen, it is standby；
Commercially available qualified food sodium hydroxide, high-efficient activated clay are chosen, it is standby；
The compound bean product defoamer of Zhengzhou high aim Food Co., Ltd production is chosen, it is standby；
Choose composite flocculation agent I, the composite flocculation agent II, composite flocculation agent of Weifang clear water Chemical Co., Ltd. production
No. I with composite flocculation agent II in proportion 1:1 is used as flocculant, and the solution of concentration 25% is made respectively, standby；
(2), immersion, the processing of acid adjustment basicity
Defatted soybean meal in step (1) is sent into enzymatic vessel, the deionized water for adding 8 times is soaked 1 hour, is warming up to 80 DEG C,
The compound bean product defoamer selected by step (1) is added, is stirred, is then added while stirring selected by step (1)
Food sodium hydroxide regulation PH is 8.5；Insulation 60 minutes；Wherein, it is dregs of beans weight to be combined the consumption of bean product defoamer
0.1%；
(3), digest
By the defatted soybean meal after immersion, the processing of acid adjustment basicity in step (2), reduction temperature adds step to 50 DEG C（1）In
Flavor protease, papain, the alkali protease of selection, constant temperature are digested 3 hours, and defatted soybean meal enzymolysis liquid is made；Wherein,
Food flavor enzyme with enzyme amount is defatted soybean meal weight 2%, papain with 1% that enzyme amount is defatted soybean meal weight, alkaline egg
White enzyme with 1% that enzyme amount is defatted soybean meal weight；
(4), go out enzyme
Obtained defatted soybean meal enzymolysis liquid in step (3) is heated to 80 DEG C, 45 minutes is incubated, carries out destroy the enzyme treatment, obtain
The defatted soybean meal enzymolysis liquid of enzyme-deactivating；
(5), flocculation purification
The defatted soybean meal enzymolysis liquid of the enzyme-deactivating of gained in step (4) is transferred to flocculation tank, added selected in step (1)
The high-efficient activated clay taken, is stirred, and the flocculant produced in step (1) is then added while stirring, 30 are staticly settled
Minute, will be digested in defatted soybean meal enzymolysis liquid remaining dregs of beans flocculated by flocculant, atlapulgite adsorption precipitation to tank bottom；Take wadding
The clear liquid on solidifying tank upper strata, obtains soy glycoproteins clear liquid；Wherein, the consumption of high-efficient activated clay is the 8% of defatted soybean meal weight, wadding
The consumption of solidifying agent is the 3% of defatted soybean meal weight；
(6), carbon is de-, filter
The soy glycoproteins clear liquid of gained in step (5) is transferred to carbon and takes off tank, the activated carbon of its weight 3% is added, heated up
To 85 DEG C, stirring insulation 60 minutes carries out the de- clarification of carbon, sends into filter centrifugal, control rotating speed 20000rpm, filter 5 minutes,
Obtain soy glycoproteins filtered fluid；
(7), concentrate, be spray-dried
Soy glycoproteins filtered fluid obtained by step (6) is concentrated, is re-fed into spray dryer and is sprayed
Mist is dried, and obtains soy glycoproteins；The condition of work of spray drying is：Inlet temperature is 230 DEG C, and outlet temperature is 82 DEG C, atomization
Disk rotating speed is located in 48Hz, tower negative pressure in 10Pa.
The method that the utilization defatted soybean meal that the present embodiment is provided produces soy glycoproteins, its enzymolysis process mild condition,
Easy control of reaction；Its flocculation purification technique sloughs the impurity in solution, its carbon is de- to carry out decolouring clarification to clear liquid, improves to obtain thing
Purity.Its preparation method technique is advanced, and rationally, operation is controllable, is suitable for large-scale production for process.
Embodiment 4
A kind of method that utilization defatted soybean meal produces soy glycoproteins, by following process steps：
(1), the selection and processing of raw material
It is raw material to choose the remaining defatted soybean meal of selection soybean grease factory squeezing grease, standby；
Flavor protease, papain are chosen, it is standby；
Commercially available qualified food sodium hydroxide, high-efficient activated clay are chosen, it is standby；
The soybean extraction defoamer of Shanghai Chemical Co., Ltd. of Hang Seng production is chosen, it is standby；
The polyacrylamide of the happy peace and quiet water Materials Co., Ltd production in Henan Gongyi City is chosen as flocculant, it is standby；
(2), immersion, the processing of acid adjustment basicity
Defatted soybean meal in step (1) is sent into enzymatic vessel, the deionized water for adding 5 times is soaked 2 hours, is warming up to 80 DEG C,
Soybean extraction defoamer selected in step (1) is added, stirs, then adds what is chosen in step (1) while stirring
Food sodium hydroxide regulation PH is 8.7；Insulation 45 minutes；Wherein, the consumption of soybean extraction defoamer is dregs of beans weight
0.25%；
(3), digest
By the defatted soybean meal after immersion, the processing of acid adjustment basicity in step (2), reduction temperature adds step to 45 DEG C（1）In
Flavor protease, the papain of selection, constant temperature are digested 4 hours, and defatted soybean meal enzymolysis liquid is made；Wherein, flavor protease
With 2% that enzyme amount is defatted soybean meal weight；Papain with 1% that enzyme amount is defatted soybean meal weight；
(4), go out enzyme
Obtained defatted soybean meal enzymolysis liquid in step (3) is heated to 85 DEG C, 30 minutes is incubated, carries out destroy the enzyme treatment, obtain
The defatted soybean meal enzymolysis liquid of enzyme-deactivating；
(5), flocculation purification
The defatted soybean meal enzymolysis liquid of the enzyme-deactivating of gained in step (4) is transferred to flocculation tank, added selected in step (1)
The high-efficient activated clay taken, is stirred, and flocculant selected in step (1) is then added while stirring, 50 are staticly settled
Minute, will be digested in defatted soybean meal enzymolysis liquid remaining dregs of beans flocculated by flocculant, atlapulgite adsorption precipitation to tank bottom；Take wadding
The clear liquid on solidifying tank upper strata, obtains soy glycoproteins clear liquid；Wherein, the consumption of high-efficient activated clay is the 7% of defatted soybean meal weight, wadding
The consumption of solidifying agent is the 3% of defatted soybean meal weight；
(6), carbon is de-, filter
The soy glycoproteins clear liquid of gained in step (5) is transferred to carbon and takes off tank, the activated carbon of its weight 5% is added, heated up
To 85 DEG C, stirring insulation 45 minutes carries out the de- clarification of carbon, and filter-cloth filtering obtains soy glycoproteins filtered fluid；
(7), concentrate, be spray-dried
Soy glycoproteins filtered fluid obtained by step (6) is concentrated, is re-fed into spray dryer and is sprayed
Mist is dried, and obtains soy glycoproteins；The condition of work of spray drying is：Inlet temperature is 215 DEG C, and outlet temperature is 83 DEG C, atomization
Disk rotating speed is located in 48Hz, tower negative pressure in 9Pa.
The method that the utilization defatted soybean meal that the present embodiment is provided produces soy glycoproteins, its technique is advanced, and process is reasonable,
Process conditions are gentle, operate controllable, the purity height of soy glycoproteins, are suitable for large-scale production.
Claims (8)
1. a kind of method that utilization defatted soybean meal produces soy glycoproteins, it is characterised in that：By the following steps：
(1), the selection and processing of raw material
Selection defatted soybean meal is raw material, standby；
(2), immersion, the processing of acid adjustment basicity
The defatted soybean meal chosen in step (1) is sent into enzymatic vessel, the deionized water of 3~8 times of defatted soybean meal weight, immersion is added
1~2 hour, 70~80 DEG C are warming up to, defoamer is added, stirs；Then adding sodium hydroxide regulation pH while stirring is
8.5~9.5, it is incubated 10~60 minutes；Wherein, the consumption of described defoamer is the 0.1~0.3% of dregs of beans weight；
(3), digest
Defatted soybean meal after immersion, the processing of acid adjustment basicity in step (2) is reduced into temperature to 40~50 DEG C, defatted soybean meal weight is added
The protease of amount 2~4%, constant temperature is digested 3~5 hours, and defatted soybean meal enzymolysis liquid is made；
(4), go out enzyme
Obtained defatted soybean meal enzymolysis liquid in step (3) is heated to 80~85 DEG C, 10~45 minutes are incubated, progress is gone out at enzyme
Reason, obtains the defatted soybean meal enzymolysis liquid of enzyme-deactivating；
(5), flocculation purification
The defatted soybean meal enzymolysis liquid of the enzyme-deactivating of gained in step (4) is transferred to flocculation tank, atlapulgite is added, stirring is equal
It is even, flocculant is then added while stirring, is staticly settled 30~50 minutes, remaining dregs of beans will be digested in defatted soybean meal enzymolysis liquid
Flocculated by flocculant, atlapulgite adsorption precipitation to tank bottom；The clear liquid on flocculation tank upper strata is taken, soy glycoproteins clear liquid is obtained；Wherein,
The consumption of described atlapulgite is the 5~8% of defatted soybean meal weight, and the consumption of described flocculant is the 3 of defatted soybean meal weight
~4%；
(6), carbon is de-, filter
The soy glycoproteins clear liquid of gained in step (5) is transferred to carbon and takes off tank, the activated carbon of its weight 3~6% is added, heated up
To 80~85 DEG C, stirring insulation carries out the de- clarification of carbon for 30~60 minutes, and filtering obtains soy glycoproteins filtered fluid；
(7), concentrate, be spray-dried
Soy glycoproteins filtered fluid obtained by step (6) is concentrated, is re-fed into spray dryer spray and does
It is dry, obtain soy glycoproteins.
2. the method that a kind of utilization defatted soybean meal according to claim 1 produces soy glycoproteins, it is characterised in that：It is described
Protease be compound protease, flavor protease, papain, alkali protease in one or more.
3. the method that a kind of utilization defatted soybean meal according to claim 1 produces soy glycoproteins, it is characterised in that：It is described
Atlapulgite be high-efficient activated clay.
4. the method that a kind of utilization defatted soybean meal according to claim 1 produces soy glycoproteins, it is characterised in that：It is described
Sodium hydroxide be food sodium hydroxide.
5. the method that a kind of utilization defatted soybean meal according to claim 1 produces soy glycoproteins, it is characterised in that：It is described
Defoamer be commercially available bean product defoamer.
6. the method that a kind of utilization defatted soybean meal according to claim 1 produces soy glycoproteins, it is characterised in that：It is described
Step（6）In be filtered into filter-cloth filtering or centrifugal filtration.
7. the method that a kind of utilization defatted soybean meal according to claim 6 produces soy glycoproteins, it is characterised in that：It is described
Centrifugal filtration be control centrifuge rotating speed be 4000rpm~20000rpm, centrifugation time be 5~15 minutes.
8. the method that a kind of utilization defatted soybean meal according to claim 1 produces soy glycoproteins, it is characterised in that：It is described
The condition of work of spray drying be：Inlet temperature is 200~230 DEG C, and outlet temperature is 82~85 DEG C, and atomizing disk rotating speed is located at
Negative pressure is in 8~10Pa in 48Hz, tower.
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| CN101317623A (en) * | 2008-07-04 | 2008-12-10 | 华南理工大学 | Preparation method for oil-in-water type soybean isolated protein |
| CN103142682A (en) * | 2013-02-28 | 2013-06-12 | 惠州市九惠制药股份有限公司 | Method for extracting liquorice flavonoids from liquorice residue |
| CN103271220A (en) * | 2013-05-10 | 2013-09-04 | 安徽强旺调味食品有限公司 | Method for preparing enhanced flavour peptide by using high-temperature bean pulp |
| CN103988975A (en) * | 2014-05-23 | 2014-08-20 | 东北农业大学 | Method for extracting protein from soybean meal by adopting ultrasonic-assisted reverse micelle extraction process |
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