CN104502290A - Detection method of sarcandra glabra medicinal material - Google Patents
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Abstract
The invention discloses a detection method of a sarcandra glabra medicinal material. The detection method comprises the following steps: (1) preparing a reference solution; (2) preparing a standard curve, namely measuring the reference solution, determining the absorbance, and drawing the standard curve by taking the absorbance as a vertical coordinate and the concentration as a horizontal coordinate; (3) preparing a sample solution; (4) measuring the sample solution, determining the absorbance, reading the quantity of gallate acid in the sample solution from the standard curve, and calculating to obtain the content of total phenol; and (6) calculating the content of tannin in the sample solution. According to the detection method, the quality of the sarcandra glabra medicinal material can be well controlled, the quality control standard of the sarcandra glabra medicinal material is further improved, and the stability, uniformity and controllability of the quality of the sarcandra glabra medicinal material are guaranteed; meanwhile, the extraction efficiency of the tannin as well as the accuracy of a result are further improved, and the examination task time is saved.
Description
Technical field
The present invention relates to a kind of determination method of Chinese crude drug, be specifically related to a kind of detection method of sarcandra medicinal material, belong to Chinese crude drug analysis technical field.
Background technology
Sarcandra has another name called synthetism tree, nine joint flower, Herba Pileae Scriptaes etc., for the dry aerial parts of Chloranthaceae plant plait coral Sarcandra glabra (Thunb.) Nakai, mainly being distributed in the provinces and regions such as the Guangdong on the south China the Changjiang river, Guangxi, Jiangxi, Fujian, Hunan, Zhejiang, is perennial evergreen draft or semishrub plant.The medicinal history of sarcandra is long, successive dynasties medical book its medical value widely all on the books.It has clearing heat and cooling blood, anti-inflammatory analgetic, ecchymose removing of invigorating blood circulation, dispel rheumatism, the effect such as to stimulate the circulation of the blood and cause the muscles and joints to relax, for influenza, anemopyretic cold, sphagitis, pneumonia, bacillary dysentery, acute gastroenteritis, appendicitis, sore abscess, cellulitis etc.
Sarcandra is containing compositions such as phenols, tannin, flavonoid glycoside, cumarin, organic acid, lactones, and wherein tannin (tannins) is also known as tannin, is the polyhydric phenols being extensively present in a class formation more complicated in plant.Less to Quality Research of tanning in the past, think that useless impurity is removed more, in recent years, along with the progress of analysis, separation means, progressively find that tannin has anti-lipid peroxidation, the multiple pharmacologically active such as antibacterial, antiviral, antitumor, make it day by day come into one's own in clinical practice, and the content assaying method of tannin is had higher requirement.At present, there is more report about the assay of the composition such as isofraxidin, Rosmarinic acid in sarcandra medicinal material, but there is not yet bibliographical information about the assay of tannin composition in this medicinal material.Content of tannin assay method has Casein Method, hide powder method, permanganimetric method, compleximetry etc., and wherein the most frequently used is Casein Method.Casein Method is the content of tannin assay method that " Chinese Pharmacopoeia " version one in 2010 is recorded, but in this determination method, used reagent " casein " is not clearly defined, the casein of the different batches of the casein that different manufacturer produces and same manufacturer production is all very large on the impact of content of tannin measurement result, cause the poor repeatability of measurement result, accuracy low, thus have influence on the quality controllability of sarcandra medicinal material.In addition, need place and spend the night, operation duration when in this assay method prepared by need testing solution, tannin is extracted not exclusively, also has influence on the accuracy of measurement result.
Summary of the invention
For the deficiencies in the prior art, object of the present invention aims to provide a kind of detection method of sarcandra medicinal material, above-mentioned detection method has workable, reproducible, that accuracy is high, the operation used time is short feature, above-mentioned detection method can control the quality of sarcandra medicinal material well, further increase its quality control standard, ensure the stable, homogeneous, controlled of its quality.Meanwhile, the method further increases the extraction efficiency of tannin and the accuracy of result, when saving inspection man.
For achieving the above object, the present invention adopts following technical scheme:
A detection method for sarcandra medicinal material, is characterized in that, comprises the steps (following steps answer lucifuge to operate):
1) preparation of reference substance solution: precision takes gallic acid reference substance 50mg, put in the brown measuring bottle of 100ml, be dissolved in water and be diluted to scale, precision measures 5ml, put in the brown measuring bottle of 50ml, be diluted with water to scale, shake up, obtain reference substance solution, containing gallic acid 0.05mg in every 1ml reference substance solution;
2) preparation of typical curve: precision measures reference substance solution 0.5ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml, put in the brown measuring bottle of 25ml respectively, respectively add P-Mo-Wo acid test solution 1ml, add water 11.5ml, 11ml, 10ml, 9ml, 8ml, 7ml more respectively, scale is diluted to the sodium carbonate liquor that concentration is 29%, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, take absorbance as ordinate, concentration is horizontal ordinate, drawing standard curve;
3) preparation of need testing solution: get quantitative sarcandra medicinal powder, accurately weighed, put in tool plug conical flask, add water 250ml, weighed weight, through extracting, let cool, more weighed weight, the weight of less loss is supplied with water, shake up, leave standstill and make precipitation of solid material, filter, discard just filtrate 50ml, precision measures subsequent filtrate 20ml, puts in the brown measuring bottle of 100ml, be diluted with water to scale, shake up, obtain need testing solution.
4) mensuration of total phenol in need testing solution: precision measures need testing solution 2ml, put in the brown measuring bottle of 25ml, add P-Mo-Wo acid test solution 1ml, add water 10ml, scale is diluted to the sodium carbonate liquor that concentration is 29%, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, by step 2) typical curve in read the amount (mg) of gallic acid in need testing solution, calculate, obtain the content of total phenol;
5) in need testing solution not by the mensuration of polyphenol of adsorbing: precision measures need testing solution 25ml, add to and fill in the 100ml tool plug conical flask of 0.6g casein impalpable powder, close plug, put in 30 DEG C of water-baths and be incubated 1 hour, jolting constantly, take out, let cool, shake up, filter, discard just filtrate, precision measures subsequent filtrate 2ml, put in the brown measuring bottle of 25ml, add P-Mo-Wo acid test solution 1ml, add water 10ml, scale is diluted to the sodium carbonate liquor that concentration is 29%, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, by step 2) typical curve in read the amount (mg) of gallic acid in need testing solution, calculate, obtain not by the content of polyphenol adsorbed,
6) calculating of content of tannin in test sample: calculate total phenol amount in test sample respectively according to need testing solution extension rate and not by the polyphenol amount of adsorbing, then be calculated as follows the content of tannin in test sample: content of tannin=total phenol amount-not by the polyphenol amount of adsorbing.
Described sarcandra medicinal material refers to the dry aerial parts of Chloranthaceae plant plait coral Sarcandra glabra (Thunb.) Nakai, and removing impurity, clean, segment, dries.
Realize object of the present invention to reach by taking following technical scheme:
As preferably, in step 5) in, described casein impalpable powder refers to can all by No. eight sieves, and containing being no less than the casein powder of 95% by No. nine sieves.
As preferably, in step 3) in, described sarcandra medicinal powder sampling amount is 0.5g.
As preferably, in step 3) in, described extracting method is heating and refluxing extraction or heats ultrasonic process.
As preferably, in step 3) in, the time of heating and refluxing extraction is 0.5-1 hour.
As preferably, in step 3) in, the time of heating ultrasonic process is 1-2 hour.
Compared with prior art, the present invention has following beneficial effect:
1. the present invention establishes the content assaying method of tannin in a kind of energy Accurate Determining sarcandra medicinal material first, and the method can control the quality of sarcandra medicinal material well, further increases its quality control standard, ensures the stable, homogeneous, controlled of its quality.
2. the content of tannin assay method that " Chinese Pharmacopoeia " version one in 2010 is recorded adopts Casein Method, by a large amount of creationary embodiment, the present invention finds that the assay Influence on test result of reagent " casein " to tannin used in this determination method is very large.The casein of the casein adopting different manufacturer to produce and the different batches of same manufacturer production has carried out content of tannin mensuration respectively to many batches of sarcandra medicinal materials, its content of tannin measurement result deviation of casein of the different batches of the casein that result adopts different manufacturer to produce with a collection of sarcandra medicinal material respectively and same manufacturer production is very large, and then find it is that to cause the deviation of measurement result large due to the particle size differences of " casein ", when using granularity to be the casein of " impalpable powder ", measurement result is the casein of " middle powder " apparently higher than use granularity.In order to determine the correlativity of casein granularity and content of tannin measurement result further, be after the casein of middle powder is ground into impalpable powder by the granularity of former manufacturer production, with the comparative study of pulverizing previous crops, result also shows when casein granularity is for " impalpable powder ", the content of tannin measurement result of sample is apparently higher than " middle powder ", and measurement result repeatability very well.Reason is the effect of casein in this determination method is optionally adsorb tannin constituents, plays the effect of quantitative reaction, and its suction-operated power directly has influence on the accuracy of content of tannin measurement result in test sample.Due to " content of tannin=total phenol amount-not by the polyphenol amount of adsorbing ", and its particle size differences of casein that different manufacturer produces is larger, the adsorptive power of casein depends on its granule size, granularity little person specific surface area is large, to the tannin absorption in test sample completely, the assay result precision of test sample tannin is high.Its specific surface area of the large person of casein granularity is little, and suction-operated is weak, makes not higher by the polyphenol content adsorbed, thus causes the assay Lower result of test sample tannin.In the content of tannin assay method that " Chinese Pharmacopoeia " version one in 2010 is recorded, used reagent " casein " is not clearly defined, for the weak point in the method, casein granularity is clearly " impalpable powder " by the method set up in the present invention, newly-established method is reproducible, accuracy is high, improves the controllability of sarcandra quality of medicinal material.
3. need place when in the content of tannin assay method that records of " Chinese Pharmacopoeia " version one in 2010 prepared by need testing solution and spend the night, operation duration, tannin is extracted not exclusively, the preparation of need testing solution of the present invention adopts heating reflux method or heating ultrasonic method to replace the hold over night of test sample, improve the extraction efficiency of tannin and the accuracy of result, when saving inspection man.
In sum, in the sarcandra medicinal material that the present invention sets up, the content assaying method of tannin has the advantages such as workable, reproducible, accuracy is high, the operation used time is short, method can control the quality of sarcandra medicinal material well, further increase its quality control standard, ensure the stable, homogeneous, controlled of its quality.To achieve the object of the present invention, also the content assaying method of tannin in sarcandra medicinal material has been carried out to the Method validation tests such as repeatability, Intermediate precision, average recovery in invention, test findings shows, the method is reproducible, accuracy is high.In addition, the method set up in the present invention also provides good reference function to the assay of tannin in other medicinal materials and preparation thereof.
Accompanying drawing explanation
Fig. 1 is gallic acid linear relationship chart of the present invention.
Embodiment
In order to make those skilled in the art understand technical scheme of the present invention better, below by specific embodiment, the present invention is described in further detail, and these embodiments only for setting forth the present invention, instead of limit the scope of the invention.
In content assaying method provided by the invention, agents useful for same and medicinal material are commercially available, casein is chemical pure, thered is provided by Luoyang City's chemical reagent factory, Shantou Xilong Chemical Factory and Huankai Microbes Tech Co., Ltd., Guangdong respectively, sarcandra medicinal material is provided by Guangzhou Cai Zhilin pharmaceutcal corporation, Ltd, gallic acid reference substance is provided by Nat'l Pharmaceutical & Biological Products Control Institute, lot number 110831-200302.Water is ultrapure water, and other reagent is pure for analyzing.Key instrument has Japanese Shimadzu Corporation UV-2450 type ultraviolet spectrophotometer, KQ-600DE type numerical control supersonic cleaning apparatus (Kunshan Ultrasonic Instruments Co., Ltd.), Precisa 404A, 92SM-202A type electronic analytical balance etc.
Embodiment 1:
The casein of different manufacturer is on the impact of content of tannin measurement result in sarcandra medicinal material:
A detection method for sarcandra medicinal material, comprises the steps (following steps answer lucifuge to operate):
1) preparation of reference substance solution: precision takes gallic acid reference substance 50.08mg, put in the brown measuring bottle of 100ml, be dissolved in water and be diluted to scale, precision measures 5ml, put in the brown measuring bottle of 50ml, be diluted with water to scale, shake up, obtain the gallic acid reference substance solution that concentration is 0.05008mg/ml.
2) preparation of typical curve: precision measures above-mentioned reference substance solution 0.5ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml, put in the brown measuring bottle of 25ml respectively, respectively add P-Mo-Wo acid test solution 1ml, add water 11.5ml, 11ml, 10ml, 9ml, 8ml, 7ml more respectively, scale is diluted to 29% sodium carbonate liquor, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, take absorbance as ordinate, concentration is horizontal ordinate, drawing standard curve.Regression equation is Y=0.1232X+0.0517, r=0.9994, and result shows, gallic acid reference substance concentration is good linear relationship with its absorbance between 1.0016 μ g/ml-10.016 μ g/ml.See accompanying drawing 1.
3) preparation of need testing solution: get sarcandra medicinal powder and be about 0.5g, accurately weighed, put in tool plug conical flask, add water 250ml, weighed weight, adds hot reflux 1 hour, let cool, more weighed weight, the weight of less loss is supplied with water, shake up, leave standstill (making precipitation of solid material), filter, discard just filtrate 50ml, precision measures subsequent filtrate 20ml, puts in the brown measuring bottle of 100ml, be diluted with water to scale, shake up, to obtain final product.
4) mensuration of total phenol in need testing solution: precision measures need testing solution 2ml, put in the brown measuring bottle of 25ml, add P-Mo-Wo acid test solution 1ml, add water 10ml, scale is diluted to the sodium carbonate liquor that concentration is 29%, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, from typical curve, read the amount (mg) of gallic acid in need testing solution, calculate, obtain the content of total phenol;
5) in need testing solution not by the mensuration of polyphenol of adsorbing: precision measures need testing solution 25ml, add to and fill in the 100ml tool plug conical flask of 0.6g casein impalpable powder, close plug, put in 30 DEG C of water-baths and be incubated 1 hour, jolting constantly, take out, let cool, shake up, filter, discard just filtrate, precision measures subsequent filtrate 2ml, put in the brown measuring bottle of 25ml, add P-Mo-Wo acid test solution 1ml, add water 10ml, scale is diluted to the sodium carbonate liquor that concentration is 29%, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, the amount (mg) of gallic acid in need testing solution is read from typical curve, calculate, obtain not by the content of polyphenol adsorbed,
6) calculating of content of tannin in test sample: calculate total phenol amount in test sample respectively according to need testing solution extension rate and not by the polyphenol amount of adsorbing, then be calculated as follows the content of tannin in test sample: content of tannin=total phenol amount-not by the polyphenol amount of adsorbing.
Test findings shows, the content of tannin deviation that the casein (the casein granularity difference that different manufacturer produces is very large) adopting different manufacturer to produce with a collection of sarcandra medicinal material records is very large, when using granularity to be the casein of " impalpable powder ", measurement result is the casein of " middle powder " apparently higher than use granularity, shows that the assay Influence on test result of reagent " casein " to tannin used in this determination method is larger.In table 1.
The casein of the different manufacturer of table 1 (granularity) is on the impact of content of tannin measurement result in sarcandra medicinal material
Note: casein manufacturer (granularity): A. Luoyang City chemical reagent factory, cross No. 8 sieves (impalpable powder), B. Shantou Xilong Chemical Factory, cross No. 4 sieves (middle powder), C. Huankai Microbes Tech Co., Ltd., Guangdong, crosses No. 4 sieves (middle powder).
Embodiment 2:
The different batches casein of same manufacturer is on the impact of content of tannin measurement result in sarcandra medicinal material: concrete operation step is with embodiment 1, unlike in need testing solution not the casein that adopts by the mensuration of polyphenol of adsorbing be respectively the different batches casein of same manufacturer production, investigate the different batches casein of same manufacturer to the impact of content of tannin measurement result in sarcandra medicinal material.Test findings shows, the casein of the different batches of same manufacturer production is adopted to measure with the content of tannin in a collection of sarcandra medicinal material, its result error is also very large, shows that the assay Influence on test result of reagent " casein " to tannin used in this determination method is larger.In table 2.
The different batches casein of the same manufacturer of table 2 is on the impact of content of tannin measurement result in sarcandra medicinal material
Embodiment 3:
The casein of same manufacturer pulverizes the impact of front and back on content of tannin measurement result in sarcandra medicinal material: concrete operation step is with embodiment 1, unlike in need testing solution not the casein that adopts by the mensuration of polyphenol of adsorbing be respectively same manufacturer pulverize before and casein after pulverizing, the casein investigating same manufacturer pulverize before and after impact on content of tannin measurement result in sarcandra medicinal material.Test findings shows, in the sarcandra medicinal material adopting the casein (impalpable powder) after pulverizing to record, content of tannin result is apparently higher than the result adopting the casein (middle powder) before pulverizing to record, and the repeatability of measurement result is better.In table 3, table 4.
The casein of the same manufacturer of table 3 affects one to content of tannin measurement result in sarcandra medicinal material before and after pulverizing
Note: casein manufacturer (granularity): B1. Shantou Xilong Chemical Factory, crosses No. 4 sieves (middle powder) before pulverizing, B2. Shantou Xilong Chemical Factory, crosses No. 8 sieves (impalpable powder) after pulverizing.
The casein of the same manufacturer of table 4 affects two to content of tannin measurement result in sarcandra medicinal material before and after pulverizing
Note: casein manufacturer (granularity): C1. Huankai Microbes Tech Co., Ltd., Guangdong, crosses No. 4 sieves (middle powder) before pulverizing, C2. Huankai Microbes Tech Co., Ltd., Guangdong, crosses No. 8 sieves (impalpable powder) after pulverizing.
Embodiment 4:
Different need testing solution preparation method is on the impact of content of tannin measurement result in sarcandra medicinal material: concrete operation step is with embodiment 1, adopt official method (hold over night method), heating reflux method, heating ultrasonic method respectively unlike need testing solution preparation, investigate the impact of different preparation method on content of tannin measurement result in sarcandra medicinal material.Test findings shows, when the preparation of need testing solution adopts heating reflux method or heats ultrasonic method, tannin in sarcandra medicinal material is extracted more complete, and the accuracy of measurement result is higher, and adds hot reflux 0.5-1 hour and heat that ultrasonic to process the extraction effect of 1-2 hour close.In table 5.
The different need testing solution preparation method of table 5 is on the impact of content of tannin measurement result in sarcandra medicinal material
Embodiment 5:
Replica test: get with a collection of test sample 6 parts, is undertaken processing and measuring content of tannin by under " preparation of need testing solution " item.Result RSD=1.84%, shows that the repeatability of the method is good, in table 6.
Table 6 replica test result
Embodiment 6:
Intermediate precision is tested: get with a collection of test sample 6 parts, respectively by A, B, C tri-people, in the different time, with same equipment, measures by content of tannin assay method.Result precision is good, and RSD=2.22%, in table 7.
Table 7 Intermediate precision test findings
Embodiment 7:
Average recovery is tested: get the test sample 6 parts (content: 26.83mg/g) with a collection of known content, get about 0.25g for every part, accurately weighed, put in tool plug conical flask, precision adds gallic acid reference substance solution (0.5008mg/ml) 10ml respectively, remainder is undertaken processing and measuring by under " preparation of need testing solution " item, calculates the recovery.Result average recovery rate is 97.81%, RSD=1.89%, in table 8.
Table 8 average recovery test findings
Embodiment 8:
In different batches sarcandra medicinal material content of tannin measure: by the method set up in the present invention, content of tannin mensuration is carried out to 10 batches of sarcandra medicinal materials, result content of tannin between 1.23%-3.16%, in table 9.Because in different batches sarcandra medicinal material, content of tannin differs greatly, in order to avoid causing the fluctuation of content because of factors such as the medicinal material place of production, collecting season, growth year, transport storages, the a collection of basis that content of tannin is minimum in 10 batches of medicinal materials lowers 20%, specify that this product is pressed dry product and calculated accordingly, content of tannin must not be less than 1.0%.
Content of tannin measurement result in table 9 different batches sarcandra medicinal material
For a person skilled in the art, according to technical scheme described above and design, other various corresponding change and distortion can be made, and all these change and distortion all should belong within the protection domain of the claims in the present invention.
Claims (6)
1. a detection method for sarcandra medicinal material, is characterized in that, comprises the steps:
1) preparation of reference substance solution: precision takes gallic acid reference substance 50mg, put in the brown measuring bottle of 100ml, be dissolved in water and be diluted to scale, precision measures 5ml, put in the brown measuring bottle of 50ml, be diluted with water to scale, shake up, obtain reference substance solution, containing gallic acid 0.05mg in every 1ml reference substance solution;
2) preparation of typical curve: precision measures reference substance solution 0.5ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml, put in the brown measuring bottle of 25ml respectively, respectively add P-Mo-Wo acid test solution 1ml, add water 11.5ml, 11ml, 10ml, 9ml, 8ml, 7ml more respectively, scale is diluted to the sodium carbonate liquor that concentration is 29%, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, take absorbance as ordinate, concentration is horizontal ordinate, drawing standard curve;
3) preparation of need testing solution: get quantitative sarcandra medicinal powder, accurately weighed, put in tool plug conical flask, add water 250ml, weighed weight, through extracting, let cool, more weighed weight, the weight of less loss is supplied with water, shake up, leave standstill and make precipitation of solid material, filter, discard just filtrate 50ml, precision measures subsequent filtrate 20ml, puts in the brown measuring bottle of 100ml, be diluted with water to scale, shake up, obtain need testing solution.
4) mensuration of total phenol in need testing solution: precision measures need testing solution 2ml, put in the brown measuring bottle of 25ml, add P-Mo-Wo acid test solution 1ml, add water 10ml, scale is diluted to the sodium carbonate liquor that concentration is 29%, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, by step 2) typical curve in read the amount (mg) of gallic acid in need testing solution, calculate, obtain the content of total phenol;
5) in need testing solution not by the mensuration of polyphenol of adsorbing: precision measures need testing solution 25ml, add to and fill in the 100ml tool plug conical flask of 0.6g casein impalpable powder, close plug, put in 30 DEG C of water-baths and be incubated 1 hour, jolting constantly, take out, let cool, shake up, filter, discard just filtrate, precision measures subsequent filtrate 2ml, put in the brown measuring bottle of 25ml, add P-Mo-Wo acid test solution 1ml, add water 10ml, scale is diluted to the sodium carbonate liquor that concentration is 29%, shake up, place 30 minutes with corresponding reagent for blank, according to UV-VIS spectrophotometry, absorbance is measured at the wavelength place of 760nm, by step 2) typical curve in read the amount (mg) of gallic acid in need testing solution, calculate, obtain not by the content of polyphenol adsorbed,
6) calculating of content of tannin in test sample: calculate total phenol amount in test sample respectively according to need testing solution extension rate and not by the polyphenol amount of adsorbing, then be calculated as follows the content of tannin in test sample: content of tannin=total phenol amount-not by the polyphenol amount of adsorbing.
2. the detection method of sarcandra medicinal material according to claim 1, is characterized in that: in step 5) in, described casein impalpable powder refers to can all by No. eight sieves, and containing being no less than the casein powder of 95% by No. nine sieves.
3. the detection method of sarcandra medicinal material according to claim 1, is characterized in that: in step 3) in, described sarcandra medicinal powder sampling amount is 0.5g.
4. the detection method of sarcandra medicinal material according to claim 1, is characterized in that: in step 3) in, described extracting method is heating and refluxing extraction or heats ultrasonic process.
5. the detection method of sarcandra medicinal material according to claim 4, is characterized in that: in step 3) in, the time of heating and refluxing extraction is 0.5-1 hour.
6. the detection method of sarcandra medicinal material according to claim 4, is characterized in that: in step 3) in, the time of heating ultrasonic process is 1-2 hour.
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| CN112816428A (en) * | 2020-12-31 | 2021-05-18 | 四川新绿色药业科技发展有限公司 | Method for identifying standard decoction of fructus Gardeniae, parched fructus Gardeniae and parched fructus Gardeniae |
| CN112816428B (en) * | 2020-12-31 | 2023-09-08 | 四川新绿色药业科技发展有限公司 | Identification method of standard decoction of fructus gardeniae, fried fructus gardeniae and coked fructus gardeniae |
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