CN104498390A - Sludge leavening agent - Google Patents

Sludge leavening agent Download PDF

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Publication number
CN104498390A
CN104498390A CN201410706838.2A CN201410706838A CN104498390A CN 104498390 A CN104498390 A CN 104498390A CN 201410706838 A CN201410706838 A CN 201410706838A CN 104498390 A CN104498390 A CN 104498390A
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CN
China
Prior art keywords
parts
sludge
bacteria
leavening agent
fermentation agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201410706838.2A
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Chinese (zh)
Inventor
朱道辰
谢长校
黄磊
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ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd
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ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd
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Application filed by ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd filed Critical ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd
Priority to CN201410706838.2A priority Critical patent/CN104498390A/en
Publication of CN104498390A publication Critical patent/CN104498390A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Treatment Of Sludge (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a sludge leavening agent. The sludge leavening agent is prepared from the following raw materials in parts by weight: 15-25 parts of bacillus subtilis, 10-20 parts of bacillus stearothermophilus, 8-15 parts of lactic acid bacteria, 5-10 parts of silicate bacteria, 4-10 parts of nocardia, 9-16 parts of a zotobacter chroococcum, 1-6 parts of saccharomyces cerevisiae, 1-5 parts of flavobacterium oryzae, 2-3 parts of photosynthetic bacteria, 1-3 parts of streptomyces microflavus, 6-13 parts of cellulase, 5-12 parts of amylase and 5-6 parts of xylanase. The sludge leavening agent provided by the invention is environmentally friendly, pollution-free, low in operation cost, small in floor space, and capable of effectively killing pathogenic bacteria, the parasites and the like in the sludge, degrading toxic and harmful substances, accelerating sludge deodorization and dehydration and decomposing nutrition ingredients which are difficult to absorb by crops.

Description

A kind of sludge fermentation agent
Technical field
The present invention relates to a kind of lignin-sludge starter, belong to environment friendly biological technical field.
Background technology
Municipal sludge is that after urban sewage purification process is discharged, the mud stayed in treatment tank, the sewage work of day output 100,000 tons, can produce number every year with the mud of ten thousand tons.Sludge moisture content is large, and viscosity is large and the smell is awful; Wet mud also containing harmful bacterial classification such as pathogenic bacterium, parasitic ovum, can cause secondary pollution to environment.Therefore municipal sludge is developed and utilized, solve its secondary pollution to environment, become problem in the urgent need to address.
The treatment process of municipal sludge mainly contains the forms such as landfill, burning, Ocean dumping and Land_use change.But landfill appropriation of land is serious, potential soil and groundwater also can be caused to pollute; Burning disposal facility investment is large, and organism burns also can produce the highly toxic substances such as dioxin; Ocean dumping can pollution of marine environment.Because mud has obvious soil improvement action and the promoter action to plant-growth, its fermentation is made the part starting material of fertilizer, not only can increase the organic content in soil, can also nutrient chemical be saved.
Summary of the invention
Technical solution problem: for prior art Problems existing, the invention provides a kind of sludge fermentation agent, utilizes microbial strains to make, fully effectively can utilize mud, has both solved its secondary pollution problem to environment, can become soil improvement agent again.
Technical scheme: sludge fermentation agent provided by the invention, comprise the raw material of following parts by weight: subtilis 15-25 part, bacstearothermophilus 10-20 part, milk-acid bacteria 8-15 part, silicate bacteria 5-10 part, promise Ka Shi actinomycetes 4-10 part, blown-ball Azotobacter 9-16 part, S. cervisiae 1-6 part, rice Flavobacterium 1-5 part, photosynthetic bacterium 2-3 part, streptomyces microflavus 1-3 part, cellulase 6-13 part, amylase 5-12 part and zytase 5-6 part.
Described photosynthetic bacterium is Rhodopseudomonas palustris; Described streptomyces microflavus is at 1.0 hundred million more than CFU/ml; Above-mentioned raw materials is made wettable powder according to parts by weight mixing be sludge fermentation agent through absorption, press filtration, fragmentation, oven dry.
Beneficial effect: sludge fermentation agent provided by the invention, environment friendly and pollution-free, running cost is low, and floor space is little; Effectively can kill the pathogenic bacteria, parasitic ovum etc. in mud; Degraded hazardous and noxious substances, accelerate sludge deodorizing, dehydration; Decompose the nutrition that crop is difficult to absorb.
Embodiment
Mode below by embodiment further illustrates the present invention, but therefore protection scope of the present invention is not confined to following embodiment, but is limited by claims of the present invention and specification sheets.
Embodiment 1
Sludge fermentation agent, comprise the raw material of following parts by weight: subtilis 15 parts, bacstearothermophilus 10 parts, milk-acid bacteria 8 parts, silicate bacteria 5 parts, 4 parts, promise Ka Shi actinomycetes, blown-ball Azotobacter 9 parts, S. cervisiae 1 part, rice Flavobacterium 1 part, photosynthetic bacterium 2 parts, streptomyces microflavus 1 part, cellulase 6 parts, amylase 5 parts and zytase 5 parts.
Above-mentioned each raw material is made wettable powder according to parts by weight mixing be sludge fermentation agent through absorption, press filtration, fragmentation, oven dry.
Embodiment 2
Sludge fermentation agent, comprise the raw material of following parts by weight: subtilis 20 parts, bacstearothermophilus 12 parts, milk-acid bacteria 10 parts, silicate bacteria 7 parts, 6 parts, promise Ka Shi actinomycetes, blown-ball Azotobacter 11 parts, S. cervisiae 2 parts, rice Flavobacterium 2 parts, photosynthetic bacterium 2 parts, streptomyces microflavus 2 parts, cellulase 8 parts, amylase 6 parts and zytase 5 parts.Above-mentioned each raw material is made wettable powder according to parts by weight mixing be sludge fermentation agent through absorption, press filtration, fragmentation, oven dry.
Embodiment 3
Sludge fermentation agent, comprise the raw material of following parts by weight: subtilis 23 parts, bacstearothermophilus 15 parts, milk-acid bacteria 13 parts, silicate bacteria 9 parts, 8 parts, promise Ka Shi actinomycetes, blown-ball Azotobacter 13 parts, S. cervisiae 4 parts, rice Flavobacterium 3 parts, photosynthetic bacterium 3 parts, streptomyces microflavus 3 parts, cellulase 10 parts, amylase 10 parts and zytase 6 parts.Above-mentioned each raw material is made wettable powder according to parts by weight mixing be sludge fermentation agent through absorption, press filtration, fragmentation, oven dry.
Embodiment 4
Sludge fermentation agent, comprise the raw material of following parts by weight: subtilis 25 parts, bacstearothermophilus 20 parts, milk-acid bacteria 15 parts, silicate bacteria 10 parts, 10 parts, promise Ka Shi actinomycetes, blown-ball Azotobacter 16 parts, S. cervisiae 6 parts, rice Flavobacterium 5 parts, photosynthetic bacterium 3 parts, streptomyces microflavus 3 parts, cellulase 13 parts, amylase 12 parts and zytase 6 parts.Above-mentioned each raw material is made wettable powder according to parts by weight mixing be sludge fermentation agent through absorption, press filtration, fragmentation, oven dry.

Claims (3)

1. a sludge fermentation agent, its feature is being the raw material comprising following parts by weight: subtilis 15-25 part, bacstearothermophilus 10-20 part, milk-acid bacteria 8-15 part, silicate bacteria 5-10 part, promise Ka Shi actinomycetes 4-10 part, blown-ball Azotobacter 9-16 part, S. cervisiae 1-6 part, rice Flavobacterium 1-5 part, photosynthetic bacterium 2-3 part, streptomyces microflavus 1-3 part, cellulase 6-13 part, amylase 5-12 part and zytase 5-6 part.
2. sludge fermentation agent according to claim 1, is characterized in that described photosynthetic bacterium is Rhodopseudomonas palustris.
3. sludge fermentation agent according to claim 1, is characterized in that described streptomyces microflavus >=1.0 hundred million CFU/ml.
CN201410706838.2A 2014-12-01 2014-12-01 Sludge leavening agent Pending CN104498390A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410706838.2A CN104498390A (en) 2014-12-01 2014-12-01 Sludge leavening agent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410706838.2A CN104498390A (en) 2014-12-01 2014-12-01 Sludge leavening agent

Publications (1)

Publication Number Publication Date
CN104498390A true CN104498390A (en) 2015-04-08

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106007865A (en) * 2016-06-27 2016-10-12 安徽省蚌埠市中远肥业有限公司 Organic fertilizer special for rice in tillering stage and preparation method of organic fertilizer
CN106110363A (en) * 2016-08-16 2016-11-16 叶海霞 A kind of biological deodorant being applied to public lavatory
CN106116959A (en) * 2016-06-27 2016-11-16 安徽省蚌埠市中远肥业有限公司 A kind of rice seedling phase fertilizer special for organic and preparation method thereof
CN106116868A (en) * 2016-06-27 2016-11-16 安徽省蚌埠市中远肥业有限公司 A kind of Rice during Grain Filling Stage fertilizer special for organic and preparation method thereof
CN106116764A (en) * 2016-06-27 2016-11-16 安徽省蚌埠市中远肥业有限公司 A kind of single cropping Caulis Zizaniae caduciflorae fertilizer special for organic and preparation method thereof
CN106187571A (en) * 2016-06-27 2016-12-07 安徽省蚌埠市中远肥业有限公司 A kind of Oryza sativa L. long fringe phase fertilizer special for organic and preparation method thereof
CN106748211A (en) * 2016-12-29 2017-05-31 常熟市添源环保科技有限公司 A kind of ecological Organic sludge manure
CN106810355A (en) * 2016-12-29 2017-06-09 常熟市添源环保科技有限公司 A kind of preparation method of ecological Organic sludge manure
JP2018512257A (en) * 2015-09-01 2018-05-17 ジエンシー ジェイディエル エンバイロメンタル プロテクション カンパニー リミテッド Method and system for sewage treatment with zero sludge discharge
CN108504587A (en) * 2018-03-15 2018-09-07 广东必高生物科技有限公司 Creek sludge handling microorganism formulation and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
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CN102250768A (en) * 2011-06-01 2011-11-23 康源绿洲微生物技术（北京）有限公司 Method for preparing enzyme and bacterium composite agent for treating sewage and sludge
CN102351321A (en) * 2011-09-06 2012-02-15 佛山市合璟节能环保科技有限公司 Composite biological enzyme preparation for deweighting treatment of domestic sludge, and production and application method thereof
CN102985392A (en) * 2010-03-23 2013-03-20 比奥麦克斯私人控股有限公司 Treatment of organic waste
CN103319214A (en) * 2013-06-27 2013-09-25 深圳市沃嘉生物科技有限公司 Method for making biological organic fertilizer by utilizing urban domestic sludge and special-purpose leavening agent of method
CN103467146A (en) * 2013-09-09 2013-12-25 北京沃土天地生物科技有限公司 Method for producing compound organic fertilizer by using municipal sludge
CN103834592A (en) * 2014-02-28 2014-06-04 河南科技大学 Microbial starter for treating municipal sludge and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102985392A (en) * 2010-03-23 2013-03-20 比奥麦克斯私人控股有限公司 Treatment of organic waste
CN102250768A (en) * 2011-06-01 2011-11-23 康源绿洲微生物技术（北京）有限公司 Method for preparing enzyme and bacterium composite agent for treating sewage and sludge
CN102351321A (en) * 2011-09-06 2012-02-15 佛山市合璟节能环保科技有限公司 Composite biological enzyme preparation for deweighting treatment of domestic sludge, and production and application method thereof
CN103319214A (en) * 2013-06-27 2013-09-25 深圳市沃嘉生物科技有限公司 Method for making biological organic fertilizer by utilizing urban domestic sludge and special-purpose leavening agent of method
CN103467146A (en) * 2013-09-09 2013-12-25 北京沃土天地生物科技有限公司 Method for producing compound organic fertilizer by using municipal sludge
CN103834592A (en) * 2014-02-28 2014-06-04 河南科技大学 Microbial starter for treating municipal sludge and preparation method thereof

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018512257A (en) * 2015-09-01 2018-05-17 ジエンシー ジェイディエル エンバイロメンタル プロテクション カンパニー リミテッド Method and system for sewage treatment with zero sludge discharge
CN106007865A (en) * 2016-06-27 2016-10-12 安徽省蚌埠市中远肥业有限公司 Organic fertilizer special for rice in tillering stage and preparation method of organic fertilizer
CN106116959A (en) * 2016-06-27 2016-11-16 安徽省蚌埠市中远肥业有限公司 A kind of rice seedling phase fertilizer special for organic and preparation method thereof
CN106116868A (en) * 2016-06-27 2016-11-16 安徽省蚌埠市中远肥业有限公司 A kind of Rice during Grain Filling Stage fertilizer special for organic and preparation method thereof
CN106116764A (en) * 2016-06-27 2016-11-16 安徽省蚌埠市中远肥业有限公司 A kind of single cropping Caulis Zizaniae caduciflorae fertilizer special for organic and preparation method thereof
CN106187571A (en) * 2016-06-27 2016-12-07 安徽省蚌埠市中远肥业有限公司 A kind of Oryza sativa L. long fringe phase fertilizer special for organic and preparation method thereof
CN106110363A (en) * 2016-08-16 2016-11-16 叶海霞 A kind of biological deodorant being applied to public lavatory
CN106748211A (en) * 2016-12-29 2017-05-31 常熟市添源环保科技有限公司 A kind of ecological Organic sludge manure
CN106810355A (en) * 2016-12-29 2017-06-09 常熟市添源环保科技有限公司 A kind of preparation method of ecological Organic sludge manure
CN108504587A (en) * 2018-03-15 2018-09-07 广东必高生物科技有限公司 Creek sludge handling microorganism formulation and preparation method thereof

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