CN104480045B - One plant of otitis pseudomonas strains and its application - Google Patents
One plant of otitis pseudomonas strains and its application Download PDFInfo
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- 241000589516 Pseudomonas Species 0.000 title claims description 8
- 208000005141 Otitis Diseases 0.000 title claims description 6
- 208000019258 ear infection Diseases 0.000 title claims description 6
- 241001343452 Pseudomonas otitidis Species 0.000 claims abstract description 7
- 244000005700 microbiome Species 0.000 claims abstract description 7
- 239000002351 wastewater Substances 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 30
- 230000001580 bacterial effect Effects 0.000 abstract description 30
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 15
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 12
- 239000010865 sewage Substances 0.000 abstract description 12
- 239000003643 water by type Substances 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 229910002651 NO3 Inorganic materials 0.000 description 6
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 238000005273 aeration Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000006396 nitration reaction Methods 0.000 description 4
- 239000007836 KH2PO4 Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 229910021577 Iron(II) chloride Inorganic materials 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229920011532 unplasticized polyvinyl chloride Polymers 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
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- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses one plant of high-efficiency aerobic denitrification bacterial strain for having heterotrophic nitrification function concurrently (Pseudomonas otitidi) YXHF 2, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved in, deposit number is CGMCC No：10047.The invention also discloses applications of the above-mentioned bacterial strains YXHF 2 in waste water control.The high-efficiency aerobic denitrification bacterial strain YXHF 2 disclosed by the invention for having heterotrophic nitrification function concurrently can effectively reduce ammonia nitrogen in sewage, total nitrogen and COD content, have higher application value in sanitary sewage and other various polluted-waters are administered.
Description
Technical field
The invention belongs to technical field of environmental microorganism, more particularly to one plant of high-efficiency aerobic for having heterotrophic nitrification function concurrently
Denitrification bacterial strain and its application in waste water control.
Background technology
Nitrogen is to cause body eutrophication and a critically important pollution factor of environmental pollution, and bio-denitrification technology is mesh
Preceding most widely used polluted by nitrogen control method, traditional theory thinks that biological denitrificaion must be by 2 independences of nitrification and denitrification
Process realizes that nitrification and denitrification can not occur simultaneously, nitration reaction is carried out under conditions of aerobic, and anti-nitration reaction is needed
To be carried out under conditions of strict anaerobism or anoxic, traditional two-part biological denitrification process generally existing capital expenditure and fortune
Row is costly, and energy consumption is big, the deficiency such as operational control complicated condition.
It is theoretical that traditional biological denitrificaion has been broken in the discovery of aerobic denitrification and heterotrophic nitrification so that synchronous nitration and anti-nitre
Change is possibly realized, and not only nitrification and denitrification can be made to be completed in same reactor, accelerates whole course of reaction, shortens water
The power residence time, reduce reactor volume, reduce operating cost, the water quality scope for carrying out denitrogenation processing can also be expanded.
Have the aerobic denitrifying bacteria of heterotrophic nitrification function concurrently by separation, further investigate the growth characteristics of the bacterium, anti-nitre
Change the application in characteristic and its sewage water denitrification, have important theory value to the denitrogenation processing efficiency and economy for improving sewage
And practical significance.
The content of the invention
An object of the present invention is to provide one plant of aerobic denitrifying bacteria for having heterotrophic nitrification function concurrently.
The second object of the present invention is to provide the above-mentioned aerobic denitrifying bacteria for having heterotrophic nitrification function concurrently in waste water control
In application.
To realize object above, the different aerobic denitrifying bacteria YXHF-2 for having heterotrophic nitrification function concurrently disclosed by the invention,
It is identified as Pseudomonas otitidi (NCBI accession number：KF159806.1), pseudomonas (Psedomonas is belonged to
Sp.) YXHF-2 is for the aerobic denitrifying bacteria (Pseudomonas otitidi) disclosed by the invention for having heterotrophic nitrification function concurrently
China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on November 21st, 2014, deposit number is
CGMCC No：10047.
Controlled the invention also discloses the above-mentioned high-efficiency aerobic denitrification bacterial strain YXHF-2 for having heterotrophic nitrification function concurrently in sewage
Applied in reason.
The process form of the above-mentioned high-efficiency aerobic denitrification bacterial strain YXHF-2 applications for having heterotrophic nitrification function concurrently is biological for film
Reactor.
The high-efficiency aerobic denitrification bacterial strain YXHF-2 dosages for having heterotrophic nitrification function concurrently hold for membrane bioreactor
Long-pending 1-4%.
The high-efficiency aerobic denitrification bacterial strain YXHF-2 for having heterotrophic nitrification function concurrently of the present invention can be reduced effectively in sewage
The content of ammonia nitrogen, total nitrogen and COD, has higher application value in sanitary sewage and other various polluted-waters are administered.Fortune
Use in membrane bioreactor, can effectively reduce ammonia nitrogen in membrane bioreactor sanitary sewage, total nitrogen and COD content, it is right
Exploitation denitrification microorganism preparation or sewage-treating agent have broad application prospects.
Preservation information
What the present invention was provided has the high-efficiency aerobic denitrification bacterial strain (Pseudomonas otitidi) of heterotrophic nitrification function concurrently
YXHF-2, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address：Court of Beijing
The positive institute 3 of area's North Star West Road 1, deposit number is CGMCC No：10047, preservation date is on November 21st, 2014.
Brief description of the drawings
Fig. 1 is membrane bioreactor structural representation in embodiment；
Fig. 2 is bacterial strain YXHF-2 to nitrate and the removal effect figure of total nitrogen；
Fig. 3 is removal effect figures of the bacterial strain YXHF-2 to ammonia nitrogen；
Fig. 4 is the situation of change that bacterial strain YXHF-2 adds to membrane bioreactor pollutants removal rate.
Embodiment
With reference to specific embodiment, the present invention is described in detail.
Experimental method in following embodiments, is conventional method unless otherwise specified.Examination used in following embodiments
Material is tested, is to be commercially available from routine biochemistry reagent shop unless otherwise specified.
1st, the acquisition of bacterial strain and performance detection
Enrichment culture：The sludge suspension of certain membrane bioreactor of the sample of strain isolation from Shenzhen Guanlan town, should
Device uses " sloping plate deposition+membrane bioreactor " group technology, and treating capacity is 25m3/ d, the residence time is 4h, gas-water ratio 30：
1.Membrane bioreactor sludge suspension sample 5% is seeded to enriched liquid culture medium (KNO by volume32g, KH2PO41g,
K2HPO41g, MgSO4·7H2O 0.2g, glucose 5g, distilled water 1000mL), shaken in 30 DEG C of rotating speeds for 150r/min constant temperature
Shaken cultivation 48 hours on bed.
Wherein, certain membrane bioreactor structure is shown in accompanying drawing 1.It is the membrane biological reaction of one plant of aerobic synchronous nitration and denitrification
Device device, using " sloping plate deposition+membrane bioreactor " group technology, mainly by settling zone (11), film biological treatment zone (21)
With the part of dynamic Control area (31) three composition, main equipment material is steel construction, makees depth anti-corrosion.Settling zone (11) sets one
Individual or multiple swash plates (12), mainly carry out precipitation pretreatment to the suspension of bulky grain.Film biological treatment zone (21) mainly includes
Membrane module (23), micropore aeration pipe (22) etc., wherein membrane module (23) are made up of membrane component (231) and collector pipe (232), described
Membrane module (23) is made up of one or more membrane components (231) and collector pipe (232), the collector pipe (232) and membrane component
(231) connect.Wherein membrane component (231) is PVDF material Flat Membranes, and membrane aperture is 0.2 μm, membrane flux Q=400~550L/
Piece d), collector pipe (232) is UPVC materials；Micropore aeration pipe (22) is distributed in bottom and the connection of film biological treatment zone (21)
On 1/3 wall of bottom, aeration tube aperture is 6mm, and axially spaced-apart is 60~80mm, and radial aperture angle is 140 degree.Power and control
Area processed is mainly made up of water pump (32), air pump (33) and PLC automatic controllers (34) etc., to the waterpower of film bioreactor device
The parameters such as residence time, gas-water ratio and water-out manners are automatically controlled.
Primary dcreening operation：The enriched medium after concussion and cultivate is taken to dilute 10 step by step respectively3、104With 105Times, it is coated on BTB solids
Selective agar medium (KNO31g, KH2PO4 1g, CaCl2·7H2O 0.2g, FeCl2·6H2O 0.5g, glucose 5g, volume ratio
For 1% BTB ethanol solution 1mL, agar 20g；Distilled water 1000mL, regulation pH be 7.0~7.3), be placed in 30 DEG C it is incubated
Case culture 3 days.The single bacterium colony that picking makes BTB culture mediums that blueness is presented rules three acquisitions of purifying just on rich solids culture medium
Sieve bacterial strain.
Secondary screening：Above-mentioned primary dcreening operation bacterial strain is seeded in enriched liquid culture medium respectively, trained under the conditions of 150r/min, 30 DEG C
Support 2 days.Respectively by nutrient solution by volume 5% add to denitrification detection culture medium (glucose 0.5g, sodium acetate 0.35g,
KH2PO40.105g, KNO30.3g, distilled water 1000mL), cultivated under the conditions of 150r/min, 30 DEG C, timing detection nitrate
With the concentration of total nitrogen, obtaining has the preferable bacterial strain of denitrification, is named as bacterial strain YXHF-2, YXHF-2 pairs of the bacterial strain
Nitrate and total nitrogen removal effect are shown in Fig. 2；Bacterial strain YXHF-2 after screening is added to nitrification detection culture medium (NH4Cl again
0.344g/L, glucose 1g/L, MgSO47H2O 0.2g/L, K2HPO4 0.2g/L, NaCl 0.12g/L, MnSO44H2O
0.01g/L, FeSO4 0.01g/L, pH7.0~7.2), the change in concentration of periodic detection ammonia nitrogen, as shown in Figure 3.
From Figure 2 it can be seen that bacterial strain YXHF-2 is higher to the removal effect of nitrate and total nitrogen, 24h can remove respectively
91.1% and 73.6% nitrate and total nitrogen；As seen from Figure 3, bacterial strain YXHF-2 also has preferable removal effect to ammonia nitrogen,
24h can remove 87.2% or so ammonia nitrogen.It can be seen that aerobic denitrifying bacteria YXHF-2 is respectively provided with preferably to nitrate, total nitrogen, ammonia nitrogen
Removal effect, can apply in sewage disposal.
2nd, the molecular biology identification of bacterial strain
Using " the MiniBEST Bacterial Genomic DNA Extraction of precious bioengineering Co., Ltd
Kit " kits extract bacterial strain YXHF-2 genomic DNA, and as template amplification 16SrDNA, primer：16S rDNA-
27f:5 '-AGAGTTTGATCCTGGCTCAG-3 ', 1492r (5 '-GGTTACCTTGTTACGACTT-3 '.PCR reaction systems (50
μL)：Template DNA 50-100ng, PCR Premix 25 μ L, each 0.5 μ L of upstream and downstream primer, plus ddH2O to reaction system be 50 μ
L.PCR programs：94℃10min,94℃1min,50℃1min,72℃2min,72℃5min；72℃10min；30 circulations.
The purifying and sequencing of PCR primer are completed by Huada gene company, and sequencing sequence logs in GenBank and carries out homology sequence comparison point
Analysis.
Bacterial strain YXHF-2 sequence length is 1497bp, sees gene order table.By comparing, the sequence and ncbi database
Pseudomonas otitidis (NCBI accession number：KF159806.1) homology reaches 99%, belongs to pseudomonad
Belong to (Psedomonas sp.).It is named as the high-efficiency aerobic denitrification bacterial strain (Pseudomonas for having heterotrophic nitrification function concurrently
otitidi)YXHF-2。
3rd, application effects of the high-efficiency aerobic denitrification bacterial strain YXHF-2 of heterotrophic nitrification function in membrane bioreactor is had concurrently
Really
Culture YXHF-2, shaking speed 150r/min, 30 are expanded in constant-temperature table using above-mentioned enriched liquid culture medium
3d is cultivated under the conditions of DEG C.Nutrient solution is added into centrifuge tube and 10min is centrifuged with 6000r/min, supernatant is abandoned and uses physiological saline weight
Outstanding thalline, suspension OD600About 1.6.
Bacteria suspension is added into membrane bioreactor according to 2% volume ratio, the membrane bioreactor is main by efficiently sinking
Shallow lake area and film biological treatment zone composition, intake as sanitary sewage raw water, treatment scale is 1m3/ d, hydraulic detention time is 6h, is exposed
Gas mode is continuous aeration, and gas-water ratio is 30：1, water temperature is 25~30 DEG C.Film is biological before and after monitoring adds bacterial strain YXHF-2 suspensions
COD, ammonia nitrogen and the nitrogen removal rate situation of change of reactor are shown in Fig. 4.
From fig. 4, it can be seen that YXHF-2 bacteria suspensions are added to membrane bioreactor, COD, ammonia nitrogen and total nitrogen clearance have
The clearance increase rate of different degrees of raising, wherein total nitrogen is maximum, and the nitrogen removal rate ratio for adding latter 10 days is carried when adding
It is high by 34.2%；Add and 19.0%, COD clearances raising 10.2% is improved when the ammonia nitrogen removal frank ratio after latter 10 days is added.
As can be seen from the above-described embodiment, bacterial strain YXHF-2 not only possesses excellent aerobic denitrification capability, while also having
There is heterotrophic nitrification and remove COD, there is higher answer in sanitary sewage and other various polluted-waters are administered
With value.Apply in membrane bioreactor, can effectively reduce ammonia nitrogen in membrane bioreactor sanitary sewage, nitrate nitrogen and
COD content, has broad application prospects to exploitation denitrification microorganism preparation or sewage-treating agent.
In summary, but the invention is not limited in above-mentioned embodiment, persons skilled in the art are in institute of the present invention
In the technical scope of exposure, the change that can be readily occurred in, within protection scope of the present invention.
SEQUENCE LISTING
<110>Shengang CEEUSRO Environmental Protection Engineering Technology Co., Ltd., Shenzhen
Shenzhen-Hong Kong Production ＆ Research Base
<120>A kind of high-efficiency aerobic denitrification bacterial strain for having heterotrophic nitrification function concurrently and its application
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1497
<212> DNA
<213> Pseudomonas otitidi
<400> 1
agagtttgat cctggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgagc 60
ggatgagtgg agcttgctcc atgattcagc ggcggacggg tgagtaatgc ctaggaatct 120
gcctggtagt gggggataac gtttcgaaag gaacgctaat accgcatacg tcctacggga 180
gaaagtgggg gatcttcgga cctcacgcta tcagatgagc ctaggtcgga ttagctagtt 240
ggtggggtaa tggcccacca aggcgacgat ccgtaactgg tctgagagga tgatcagtca 300
cactggaact gagacacggt ccagactcct acgggaggca gcagtgggga atattggaca 360
atgggcgaaa gcctgatcca gccatgccgc gtgtgtgaag aaggtcttcg gattgtaaag 420
cactttaagt tgggaggaag ggcagtaagt taataccttg ctgttttgac gttaccaaca 480
gaataagcac cggctaactt cgtgccagca gccgcggtaa tacgaagggt gcaagcgtta 540
atcggaatta ctgggcgtaa agcgcgcgta ggtggttcag caagttggat gtgaaagccc 600
cgggctcaac ctgggaattg catccaaaac tactgggcta gagtacggta gagggtggtg 660
gaatttcctg tgtagcggtg aaatgcgtag atataggaag gaacaccagt ggcgaaggcg 720
accacctgga ctgatactga cactgaggtg cgaaagcgtg gggagcaaac aggattagat 780
accctggtag tccacgccgt aaacgatgtc gactagccgt tgggatcctt gagatcttag 840
tggcgcagct aacgcgataa gtcgaccgcc tggggagtac ggccgcaagg ttaaaactca 900
aatgaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg 960
aagaacctta cctggccttg acatgcagag aactttccag agatggattg gtgccttcgg 1020
gaactctgac acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa 1080
gtcccgtaac gagcgcaacc cttgtcctta gttaccagca cctcgggtgg gcactctaag 1140
gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaagtcatc atggccctta 1200
cggccagggc tacacacgtg ctacaatggt cggtacaaag ggttgccaag ccgcgaggtg 1260
gagctaatcc cataaaaccg atcgtagtcc ggatcgcagt ctggaactcg actgcgtgaa 1320
gtcggaatcg ctagtaatcg tgaatcagaa tgtcacggtg aatacgttcc cgggccttgt 1380
acacaccgcc cgtcacacca tgggagaggg ttgctccaga agtagctagt ctaaccgcaa 1440
ggaggacggt taccacggag tgattcatga ctggggtgaa gtcgtaacaa ggtaacc 1497
Claims (4)
1. one plant of otitis pseudomonas strains (Pseudomonas otitidi) YXHF-2, is preserved in Chinese microorganism strain preservation
Administration committee's common micro-organisms center, deposit number is CGMCC No：10047.
2. otitis pseudomonas strains YXHF-2 as claimed in claim 1 is applied in waste water control.
3. application as claimed in claim 2, it is characterised in that：The process form of the otitis pseudomonas strains YXHF-2 applications
For membrane bioreactor.
4. application as claimed in claim 3, it is characterised in that：The otitis pseudomonas strains YXHF-2 dosages are that film is biological
The 1-4% of reactor volume.
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| CN104480045B true CN104480045B (en) | 2017-07-25 |
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| CN109055252A (en) * | 2017-11-13 | 2018-12-21 | 鼎正新兴生物技术（天津）有限公司 | A kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations and preparation method thereof |
| CN110697883B (en) * | 2018-07-09 | 2020-12-15 | 中国科学院生态环境研究中心 | Method for strengthening denitrification function of membrane bioreactor and slowing down membrane pollution |
| CN110217895B (en) * | 2019-05-31 | 2020-09-29 | 中国科学院微生物研究所 | Compound microbial agent for water environment treatment and application thereof |
| CN116376756B (en) * | 2023-03-09 | 2023-10-03 | 清远海贝生物技术有限公司 | Aerobic denitrifying bacteria and application thereof in sewage treatment |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061276A (en) * | 2010-11-15 | 2011-05-18 | 北京大学 | Pseudomonas sp. strain for biological denitrification under low temperature and application thereof |
| CN103497909A (en) * | 2013-08-27 | 2014-01-08 | 中国科学院成都生物研究所 | Bacterial strain JY9 and use thereof |
| CN103525730A (en) * | 2013-10-14 | 2014-01-22 | 青岛蔚蓝生物集团有限公司 | Pseudomonas otitidis strain and application thereof |
| CN103849588A (en) * | 2014-03-05 | 2014-06-11 | 北京市环境保护科学研究院 | Aerobic denitrifying bacterium and application thereof in sewage denitrification |
| CN104046582A (en) * | 2014-06-04 | 2014-09-17 | 辽宁大学 | Medium rich in Pseudomonas otitidis |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061276A (en) * | 2010-11-15 | 2011-05-18 | 北京大学 | Pseudomonas sp. strain for biological denitrification under low temperature and application thereof |
| CN103497909A (en) * | 2013-08-27 | 2014-01-08 | 中国科学院成都生物研究所 | Bacterial strain JY9 and use thereof |
| CN103525730A (en) * | 2013-10-14 | 2014-01-22 | 青岛蔚蓝生物集团有限公司 | Pseudomonas otitidis strain and application thereof |
| CN103849588A (en) * | 2014-03-05 | 2014-06-11 | 北京市环境保护科学研究院 | Aerobic denitrifying bacterium and application thereof in sewage denitrification |
| CN104046582A (en) * | 2014-06-04 | 2014-09-17 | 辽宁大学 | Medium rich in Pseudomonas otitidis |
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