CN104472455B - Large-size predacious mite collection method - Google Patents
Large-size predacious mite collection method Download PDFInfo
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- CN104472455B CN104472455B CN201410715130.3A CN201410715130A CN104472455B CN 104472455 B CN104472455 B CN 104472455B CN 201410715130 A CN201410715130 A CN 201410715130A CN 104472455 B CN104472455 B CN 104472455B
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- predatory mites
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Abstract
The invention discloses a large-size predacious mite collection method. The large-size predacious mite collection method is characterized in that (1) alcohol which has the concentration of 70%-75% and occupies about 65% of a centrifugal tube is added into the centrifugal tube; a leaf is supported by one hand and a container filled with the alcohol is rapidly and inversely buckled on mites to be captured by the other hand to finish collection; or (2) the large-size predacious mite collection method of vegetables comprises the following steps: a small-size writing brush is dipped with quantitative collection liquid containing 25%-50% of 75% alcohol and 75%-50% of glycerol; after a large-size predacious mite is stuck by the small-size writing brush, a specimen is transferred into a specimen tube to finish the collection; or (3) the large-size predacious mite collection method in weeds comprises the following steps: a self-made simple insect trap (shown as figure) is combined with a sweep net and a yellow basin; after impurities captured by the sweep net are poured into the yellow basin, the mites are sucked into an insect suction device and put into the container for containing the predacious mites, so that the collection is finished.
Description
Technical field
The present invention relates to mite class classification and ecological Studies field, the specifically method for catching of three kinds of Predatory Mites.
Background technology
Predatory Mites belong to Arthropoda arthropoda, Arachnoidea arachnida, Acari acari, true demodicid mite catalogue
Acariforms, including suction demodicid mite Superfamily bdelloidea of Prostigmata, red greatly demodicid mite Superfamily anystoidea, red demodicid mite Superfamily
Erythraeoidea, the monoid such as the Ascoidea ascoidea of Mesostigmata, phytoseiid mite Superfamily phytoseioidea.With existing
The development of generation agricultural, multiple large-scale insects are controlled by multiple prevention and controls, and mini pest and evil mite become the harm modern times
The key factor of agricultural production, as the Important Natural Enemy Predatory Mites of multiple small agricultural harmful organisms, is that current Pest control is ground
Important preventing and treating property natural enemy during studying carefully.Predatory Mites are widely distributed, ability and specificity, life that Predatory Mites tool height quarters a prey
Grow that potential is big, the feature such as the seasonal periodicity of the big hobby having the small habitat of identical with prey of predatory number and prey is consistent.
It is widely used in domestic and international scientific research.
The Predatory Mites such as large-scale Predatory Mites such as Anystisbaccarum take action speed soon mostly, and are not easy to be captured.Caught with routine
The method of catching has gathers materials on the spot, be related to the instrument that catches few the advantages of, but not only pole can to catch Predatory Mites using conventional method
Predatory Mites can be led to escape it is also possible to more manpower and materials and the consumption of time can be led to.
Content of the invention
The technical problem to be solved in the present invention is: provides the predation method of three kinds of Predatory Mites, method is simple, raw material comes
Source extensively and is easy to get, cheap, low cost, catches effect obvious.
The technical scheme is that large-scale Predatory Mites acquisition method, (one) loads concentration in centrifuge tube is 70-75%,
Account for the ethanol that centrifuge tube ratio is about 65%, a hand rest blade, the container that another handss will be equipped with ethanol tips upside down on rapidly and treats
On the demodicid mite catching, collection completes；Or the large-scale Predatory Mites acquisition method of (two) vegetable, it is 25-50%'s that small size brush pen is speckled with proportioning
75% ethanol and the quantitative collection liquid of the glycerol of 75-50%, after being stained with large-scale Predatory Mites transfer to specimen in specimen tube, have gathered
Become；Or the medium-and-large-sized Predatory Mites acquisition method of (three) weeds, use homemade pest sucking device, in conjunction with clap net and yellow basin, clap net is caught
After the debris grasped pour yellow container into, demodicid mite is sucked and inserts in the container placing Predatory Mites after pest sucking device, collection completes.
Vegetable large-scale Predatory Mites acquisition method (one): calculate by concentration proportioning, alcohol concentration is 75%.
Vegetable large-scale Predatory Mites acquisition method (two): calculate by concentration proportioning, alcohol concentration is 25%, glycerol concentration is
75%.
Weeds medium-and-large-sized Predatory Mites acquisition method (three): pest sucking device is: the first end of the clipped sample loading gun of 1# covers gauze
The first end of sample loading gun clipped with 2# afterwards connects, and the clipped sample-adding pipette tips other end of 2# passes through add clipped with 3# of sebific duct
Sample pipette tips e connect, and the clipped sample-adding pipette tips other end of 3# is sealing structure.
Sample-adding pipette tips are plastic pipe, and crocus cloth is 200 mesh, and the clipped sample-adding pipette tips of 1# are 1ml, and yellow basin interior diameter is
30cm, high 14cm.
Beneficial effects of the present invention:
Vegetable large-scale Predatory Mites acquisition method (one):
(1) tip upside down on demodicid mite to be captured using filling spirituous container, now Predatory Mites can be dipped into ethanol, and anesthesia continues
And can be killed, then centrifuge tube is just put, it is to avoid ethanol is excessive, then specimen is transferred to the specimen tube liquid for preserving specimen
In vivo, damage Predatory Mites body structure being caused because of direct collection can be avoided；
(2) the method can be applicable to gather great Ye vegetable or the upper life of other large leaf plant such as Chinese cabbage, Fructus Cucurbitae moschatae, Drymotaenium miyoshianum (Mak.) Mak. etc.
Build is larger, motion is difficult rapidly the Predatory Mites that catch, such as Anystisbaccarum.
Vegetable large-scale Predatory Mites acquisition method (two): be suitable to be stained with large-scale Predatory Mites, can quickly complete during seizure, the method can
It is applied to gather and give birth at lobule vegetable or other microphyll such as Fructus Capsici, pinaster and plant n omicronn-leaf position such as branch, trunk injustice
The large-scale on a small quantity Predatory Mites lived.
Weeds medium-and-large-sized Predatory Mites acquisition method (three):
(1) because the color of large-scale Predatory Mites is mostly cerise or peony, such as floss demodicid mite, red greatly demodicid mite, red demodicid mite, suction demodicid mite
Deng, therefore striking contrast, and the shape due to basin can be constituted with yellow, gleanings is easily poured into and difficult within the mite class short time
To climb out of；
(2) pass through pest sucking device and demodicid mite is sucked in pest sucking device, then insert in the container placing Predatory Mites, operation is rapid, simple
Easy row, the method can be applicable to all kinds of large-scale Predatory Mites that high-volume gathers in weeds；
(3) three of the above method all can save manpower, and improves catch rate, pollution-free not welding, catches for research
The industry of food demodicid mite provides reference；
(4) present invention is adapted for carrying out and popularization and application, provides more efficiently research method for agriculture small-sized pest control,
For scientific research bring one convenient.
Brief description
Fig. 1 is home-made contrivance schematic diagram used by the present invention；The clipped sample-adding pipette tips of wherein a:1#, b: gauze portion, c:
The clipped sample-adding pipette tips of 2#, d: sebific duct, the clipped sample-adding pipette tips of e:3#；
Fig. 2 is the actual pattern of home-made contrivance used by the present invention.
Specific embodiment
Three kinds large-scale Predatory Mites acquisition methods, comprise the following steps:
Vegetable large-scale Predatory Mites acquisition method (one): loading concentration in centrifuge tube is 70-75%, accounts for centrifuge tube ratio about
Ethanol for 65%, a hand rest blade, and the container that another handss will be equipped with ethanol tips upside down on rapidly on demodicid mite to be captured, this
When Predatory Mites can be dipped into ethanol, anesthesia can be killed then, and collection completes.
Vegetable large-scale Predatory Mites acquisition method (two): small size brush pen is speckled with 75% ethanol that proportioning is 25-50% and 75-
Specimen is transferred in specimen tube after being stained with large-scale Predatory Mites, collection completes by the quantitative collection liquid of 50% glycerol.
Weeds medium-and-large-sized Predatory Mites acquisition method (three): with homemade simple insect trap (material have plastic pipe, crocus cloth,
Sample-adding pipette tips), in conjunction with clap net and yellow basin, after the debris that clap net captures pour yellow basin into, demodicid mite is sucked pest sucking device rearmounted
Enter to place in the container of Predatory Mites, collection completes.
Embodiment 1:
Vegetable large-scale Predatory Mites acquisition method (one): loading concentration in centrifuge tube is 75%, accounts for centrifuge tube ratio and is about
65% 75% ethanol, tips upside down on demodicid mite to be captured using filling spirituous container, now Predatory Mites can be dipped into ethanol, anesthesia
Then can be killed, then centrifuge tube just be put, it is to avoid ethanol is excessive, then specimen be transferred to the specimen tube for preserving specimen
In liquid.
Embodiment 2:
Vegetable large-scale Predatory Mites acquisition method (two): according to the characteristic of Predatory Mites, small size brush pen is speckled with a certain amount of adopting
Liquid collecting, the proportioning of collection liquid be 25%75% ethanol and 75% glycerol, quickly complete during seizure, then specimen transferred to mark
In this pipe.
Embodiment 3:
Vegetable large-scale Predatory Mites acquisition method (two): according to the characteristic of Predatory Mites, small size brush pen is speckled with a certain amount of adopting
Liquid collecting, the proportioning of collection liquid be 50% 75% ethanol and 50% glycerol, quickly complete during seizure, then specimen transferred to mark
In this pipe.
Embodiment 4:
Vegetable large-scale Predatory Mites acquisition method (two): according to the characteristic of Predatory Mites, small size brush pen is speckled with a certain amount of adopting
Liquid collecting, the proportioning of collection liquid be 75% 75% ethanol and 25% glycerol, quickly complete during seizure, then specimen transferred to mark
In this pipe.
Embodiment 5:
Weeds medium-and-large-sized Predatory Mites acquisition method (three): first repeatedly swept in weeds with clap net, net will be swept
In animal and plant residue pour in yellow basin, when Predatory Mites move in basin, now by by with homemade simple suction
Demodicid mite is sucked in pest sucking device by worm device, then inserts in the container placing Predatory Mites.The clipped sample-adding pipette tips of self-control pest sucking device: 1#
After a one end covers gauze b, sample-adding pipette tips c one end clipped with 2# connects, and the clipped sample-adding pipette tips c other end of 2# passes through glue
Pipe d sample-adding pipette tips e clipped with 3# connect, and the clipped sample-adding pipette tips e other end of 3# is sealing structure.Gauze b separates
Clipped sample-adding pipette tips a of 1# and clipped sample-adding pipette tips c of 2#, prevent acarid from entering clipped sample-adding pipette tips c of 2# and glue
In pipe d, facilitate the taking-up of acarid.
The above, the only specific embodiment of the present invention, but the protection domain of invention is not limited thereto, and any ripe
Know those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in, all should cover
Within the protection domain of invention.
Claims (5)
1. large-scale Predatory Mites acquisition method it is characterised in that: (one) load in centrifuge tube concentration be 70-75%, account for centrifuge tube ratio
Example is about 65% ethanol, and a hand rest blade, and the centrifuge tube that another handss will be equipped with ethanol tips upside down on rapidly demodicid mite to be captured
On, collection completes；Or the large-scale Predatory Mites acquisition method of (two) vegetable, small size brush pen is speckled with 75% wine that volume ratio is 1:3-1:1
Specimen is transferred in specimen tube after being stained with large-scale Predatory Mites, collection completes by essence and the quantitative collection liquid of glycerol；Or (three) weeds
Medium-and-large-sized Predatory Mites acquisition method, uses homemade pest sucking device, and in conjunction with clap net and yellow basin, the debris that clap net captures are poured into
After yellow basin, demodicid mite is sucked and inserts in the container placing Predatory Mites after pest sucking device, collection completes.
2. large-scale Predatory Mites acquisition method according to claim 1 it is characterised in that: method (): by percent by volume
Calculate, alcohol concentration is 75%.
3. large-scale Predatory Mites acquisition method according to claim 1 it is characterised in that: (two) vegetable large-scale Predatory Mites collection
Method: calculate by percent by volume, 75% ethanol accounts for 25%, and glycerol accounts for 75%.
4. large-scale Predatory Mites acquisition method according to claim 1 it is characterised in that: the medium-and-large-sized Predatory Mites of (three) weeds are adopted
Diversity method: pest sucking device is: clipped sample-adding pipette tips a one end is connected with clipped sample-adding pipette tips c one end after covering gauze b,
The clipped sample-adding pipette tips c other end is connected with clipped sample-adding pipette tips e by sebific duct d, and clipped sample-adding pipette tips e are another
Hold as sealing structure.
5. large-scale Predatory Mites acquisition method according to claim 4 it is characterised in that: sample-adding pipette tips be plastic pipe, spun yarn
Cloth is 200 mesh, and clipped sample-adding pipette tips a are 1ml, and yellow basin interior diameter is 30cm, high 14cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410715130.3A CN104472455B (en) | 2014-12-02 | 2014-12-02 | Large-size predacious mite collection method |
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| CN201410715130.3A CN104472455B (en) | 2014-12-02 | 2014-12-02 | Large-size predacious mite collection method |
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| CN104472455A CN104472455A (en) | 2015-04-01 |
| CN104472455B true CN104472455B (en) | 2017-02-01 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104719269B (en) * | 2015-04-10 | 2017-06-09 | 皖南医学院 | It is a kind of to efficiently separate the method for collecting flour mite |
| CN106035236A (en) * | 2016-05-20 | 2016-10-26 | 张忠和 | Method and device for collecting cochineal raw material |
| CN106959236A (en) * | 2017-04-07 | 2017-07-18 | 合肥中保汇农生物科技有限公司 | A kind of colouring method of Tianjin river amblyseius as predatory mite digestive system |
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| CN202618079U (en) * | 2012-05-10 | 2012-12-26 | 有限公司日革研究所 | Mite catching device |
| CN103229756A (en) * | 2013-04-23 | 2013-08-07 | 福建农林大学 | Insect suction gun and use method thereof |
| CN203152336U (en) * | 2013-03-26 | 2013-08-28 | 湛江出入境检验检疫局检验检疫技术中心 | Small arthropod separator |
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|---|---|---|---|---|
| CN202618079U (en) * | 2012-05-10 | 2012-12-26 | 有限公司日革研究所 | Mite catching device |
| CN203152336U (en) * | 2013-03-26 | 2013-08-28 | 湛江出入境检验检疫局检验检疫技术中心 | Small arthropod separator |
| CN103229756A (en) * | 2013-04-23 | 2013-08-07 | 福建农林大学 | Insect suction gun and use method thereof |
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