CN104458596A - Device and method for spectral detection of content of malondialdehyde in tomato leaves in multi-angle and in-situ manner - Google Patents
Device and method for spectral detection of content of malondialdehyde in tomato leaves in multi-angle and in-situ manner Download PDFInfo
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Abstract
The invention relates to the technical field of spectral detection and particularly relates to a spectral device and method for detecting the content of malondialdehyde in tomato leaves on a tomato planting site. The device comprises a spectral probe, a light source, an omnidirectional rotating instrument and a light isolating device, wherein the omnidirectional rotating instrument consists of an arc-shaped hanging plate, a circular-ring-shaped track plate, electrically-driven rollers and support legs. The method comprises the steps: (a) preparing equipment; (b) rectifying a spectral white board; (c) carrying out automatic-angle-adjusted sampling on leaf spectra; (d) carrying out multipoint sampling on the leaf spectra; (e) calculating physicochemical values. The device is mounted on the tomato planting site and can be used for acquiring spectral data of malondialdehyde of leaves of live tomato plants; the tomato leaves can be measured in an omnidirectional and multi-angle manner; the electrically-driven rollers automatically rotate, and meanwhile, a camera cooperates with observation and monitoring, so that the detection efficiency is high.
Description
Technical field
The present invention relates to technical field of spectral detection, particularly relate to a kind of spectral device for tomato planting Site Detection tomato leaf mda content.
Background technology
Tamato fruit contains the various nutrient elements such as a large amount of carbohydrates, protein, amino acid, vitamin and inorganic salts.So tomato is welcome by common people in China very much, annual output is very large.MDA (malondialdehyde, MDA) be tomato leaf one of product that Lipid peroxidation metabolism occurs when aging or under adverse environmental factor, the number of its content can reflect the power that crop is persecuted, the degree of Lipid peroxidation metabolism can be represented, in tomato leaf aging and Resistance Physiology research, mda content is a very important index, measure other the physiological and biochemical index such as MDA accumulation in tomato plant blade to research tomato plant strain growth, monitoring growth environmental change is significant.The method of traditional mensuration mda content is thiobarbituricacidα-(TBA) colourimetry, this chemical method needs to carry out destructive experiment in laboratory just can obtain result, experimentation is time-consuming, effort, analytic process has more mechanical manipulation, and be generally not suitable for analyzing great amount of samples, the continuous monitoring of MDA in tomato leaf growth course cannot be realized, and chemical analysis needs to use chemical reagent, certainly will to environment, take into account the accuracy of determination data, simple operation and environment friendly and pollution-free characteristic, utilize spectroscopy technology substitute conventional chemical analysis method application to a certain extent.
As shown in Figure 1, because tomato leaf growthform is different, in-site measurement requires very high to light source projects angle and spectroscopic probe head incident angle, the method of existing tomato leaf MDA spectral detection adopts harvesting young leaves mostly, the fresh-keeping accuracy preventing blade from going bad to affect MDA spectral measurement of pack trash ice is high to measurement mda content requirement of real-time.
Summary of the invention
For the problems referred to above, the object of the present invention is to provide one to be applicable to Site Detection, measure a kind of multi-angle In situ spectroscopic detection tomato leaf mda content apparatus and method accurately.
For reaching above-mentioned purpose, the technical solution used in the present invention is: a kind of multi-angle In situ spectroscopic detects tomato leaf mda content device, include spectroscopic probe head, light source, its difference is, also include all-directional rotation instrument and every electro-optical device, all-directional rotation instrument is by circular arc suspension plate, ring ring plate, electricity drives roller, supporting leg forms, circular arc suspension plate arc uniform arranges row's mounting hole, light source and spectroscopic probe head select one of them mounting hole to be connected in circular arc suspension plate according to crevice projection angle and incident angle respectively, circular arc suspension plate two head end is equipped with electricity and drives roller, ring ring plate is provided with the groove of opening upwards, electricity drives in roller embedding groove and advances along impression orbit, ring ring plate bottom surface is connected with passive supporting leg, during detection, tomato strain alive is in ring ring plate center, is entered wherein by all-directional rotation instrument every electro-optical device together with tested tomato strain cover alive.
Preferably, describedly include light shade cover, support every electro-optical device, light shade cover is made by every luminescent material, and top has string holes, and cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, and string holes place is provided with sealing-plug.
Preferably, described circular arc suspension plate is also connected with camera.
Preferably, described electricity drives roller and is made up of roller and stepper motor, and stepper motor output shaft is connected with roller wheel shaft.
Preferably, the adjustable bolt regulating Circular Plate terrain clearance is provided with between described supporting leg and Circular Plate.
Preferably, described circular arc suspension plate semicircular in shape, and the scale mark representing suspension plate arc angle is marked with at sidewall.
Preferably, the mounting hole number of described circular arc suspension plate is 18, and adjacent holes angle is 10 °.
Preferably, describedly also include electric block every electro-optical device, light shade cover top is provided with suspension ring, and the suspension hook of electric block coordinates with suspension ring, and electric block is suspended on support.
Preferably, described support is three-legged support.
A kind of multi-angle In situ spectroscopic detects the method for tomato leaf MDA: comprise the steps:
A () equipment prepares: erection bracket, lifting light shade cover, set up all-directional rotation instrument and level leveling, tomato strain alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively by mounting hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover;
B () spectrum blank is corrected: blank and the concordant placement of tomato strain blade height alive, carries out the rectification of spectrum blank;
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller and starts, circular arc suspension plate is driven to turn over certain angle together with spectroscopic probe head and light source, and coordinate adjustment ring ring plate height, consistent to ensure the light path of check point by camera observation monitoring, incident angle and reflection angle are 90 °; Light source sends to first check point and detects light, and the wavelength utilizing spectroscopic probe head acquisition testing point place is 800 nm, the reflectivity of tomato leaf corresponding to 853 nm, 1158 nm, 1520 nm, 1935 nm, 2012 nm, 2012 nm, 2250 nm and 2352 nm;
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other check point;
E () physics and chemistry value calculates: by gained three point reflection rates Input Control Element respectively, control module is according to multiple linear regression equations:
Y=15.336X1+20.256X2+32.114X3-26.458X4+358.542X5-489.668X6+256.334
X7+214.789X8-395.225X9+32.15, calculate the mda content of tomato leaf, in formula, Y is mda content in tomato leaf, X1 ~ X9 is respectively the reflectivity of wavelength 800 nm, 853 nm, 1158 nm, 1520 nm, 1935 nm, 2012 nm, 2012 nm, 2250 nm and the tomato corresponding to 2352 nm strain blade alive, three check points obtain three mda content values, Y1, Y2 and Y3, Y1, Y2 and Y3 summation is made even and is mda content Y in tomato leaf.
Beneficial effect of the present invention has:
(1) all-directional rotation instrument is at tomato planting erected on site, can obtain the spectroscopic data of strain tomato leaf MDA alive, avoids because of the in vitro rear inaccurate problem of tomato leaf mda content data.
(2) circular arc suspension plate is adopted to rotate at ring ring plate level, and select different suspension angles, and tomato leaf can be measured in all-dimensional multi-angle ground, can according to the self-sow form of different tomato leaf, change spectrum crevice projection angle and incident angle easily, obtain and preferably take measurement of an angle.
(3) adjustable bolt regulates Circular Plate terrain clearance, can according to different plant growths height, and spectral preservation probe and crop distance relative constancy, to obtain preferably spectroscopic data.
(4) use the light shade cover made every luminescent material by testing tool together with tested crop cover into, stop that external environment is on the impact of spectroscopic data, it is more accurate to measure.
(5) do not lifting by crane in electro-optical device situation, electricity drives roller and automatically drives circular arc suspension plate to rotate at ring ring plate level, camera coordinates observation monitoring consistent to ensure the light path of check point simultaneously, solution drives roller without electricity and camera must repeatedly lift by crane every electro-optical device, the problem that detection efficiency is low.
(6) whole device is simple and practical, with low cost, is easy to promote.
Accompanying drawing explanation
Fig. 1 is tomato leaf schematic diagram;
Fig. 2 is all-directional rotation instrument structural representation in the embodiment of the present invention;
Fig. 3 is every electro-optical device structural representation in the embodiment of the present invention;
Fig. 4 implements in the embodiment of the present invention to measure tomato leaf mda content result;
In figure, mark indicates: 1-camera, 2-circular arc suspension plate, 3-spectroscopic probe head, 4-groove, 5-ring ring plate, 6-electricity drives roller, 7-adjustable bolt, 8-supporting leg, 9-light source, 10-support, 11-string holes, 12-light shade cover, 13-electric block, 14-light source connecting seat, 15-camera Connection Block, 16-spectroscopic probe head Connection Block, the strain alive of 17-tomato.
Embodiment
In order to understand the present invention better, below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described further, see Fig. 2, Fig. 3 and Fig. 4:
Tomato leaf mda content device is detected by multi-angle In situ spectroscopic of the invention process, Main Function facilitates erected on site, and different self-sow form tomato leaf can be suitable for, so adopt all-directional rotation instrument to hang spectroscopic probe head 3 and light source 9, to change spectrum crevice projection angle and incident angle rapidly, obtain and preferably take measurement of an angle.Stop that external environment is on the impact of spectroscopic data every electro-optical device, it is more accurate to measure.
The structure that all-directional rotation instrument is concrete: comprise circular arc suspension plate 2, ring ring plate 5, electricity drive roller 6, supporting leg 8, circular arc suspension plate 2 arc uniform arranges row's mounting hole, spectroscopic probe head 3 and light source 9 select one of them mounting hole respectively according to crevice projection angle and incident angle, are connected in circular arc suspension plate 2 respectively by light source connecting seat 14 and spectroscopic probe head Connection Block 16.Take measurement of an angle preferably to find rapidly, in the present embodiment, mounting hole adds up to 18, and every adjacent holes angle is 10 °.In order to record matching measurement result, be marked with the scale mark representing suspension plate arc angle at circular arc suspension plate 2 sidewall of semicircular in shape.Circular arc suspension plate 2 liang of head ends are equipped with electricity and drive roller 6, ring ring plate 5 is hollow annular structure, which is provided with the groove 4 of opening upwards, electricity drives roller 6 and embeds in groove 4 and advance along its track, promote circular arc suspension plate 2 to coexist ring ring plate 5 Plane Rotation together with spectroscopic probe head 3 one, pass through the mounting hole adjustment light source 9 of circular arc suspension plate 2 and the incident angle of spectroscopic probe head 3 and reflection angle is 90 ° simultaneously, reaches the object that all-dimensional multi-angle is measured.
Ring ring plate 5 bottom surface is connected with passive supporting leg 8, in order to according to different plant growths height, spectral preservation probe 3 with crop distance relative constancy, to obtain preferably spectroscopic data.Between supporting leg 8 and ring ring plate 5, the adjustable bolt 7 regulating ring ring plate 5 terrain clearance is provided with in the present embodiment.
Stop external environmental light every electro-optical device, during detection, tomato strain 17 of living is in ring ring plate 5 center, and all-directional rotation instrument covers into wherein together with the strain 17 of living of tested tomato by light shade cover 12.Describedly be made up of light shade cover 12, support 10 every electro-optical device, light shade cover 12 is made by every luminescent material, and top has string holes 11, and the cable of spectroscopic probe head 3 and light source 9 and data line pass from string holes 11, and string holes 11 place is provided with sealing-plug.Also include electric block 13 every electro-optical device, light shade cover 12 top is provided with suspension ring, and the suspension hook of electric block 13 coordinates with suspension ring, and electric block 13 hangs over the mount 10.Described support 10 is three-legged support 10.Light shade cover 12 covers above, in order to understand inner case in real time better, is provided with camera 1 in the present embodiment in circular arc suspension plate 2 by camera Connection Block 15.
During embodiment of the present invention work, detect the method for tomato leaf mda content, comprise the steps:
A () equipment prepares: erection bracket 10, lifting light shade cover 12, set up all-directional rotation instrument and level leveling, tomato strain 17 alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively by mounting hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover;
B () spectrum blank is corrected: blank and the concordant placement of tomato strain 17 blade height alive, carries out the rectification of spectrum blank;
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller 6 and starts, circular arc suspension plate 2 is driven to turn over certain angle together with spectroscopic probe head 3 and light source 9, and coordinate adjustment ring ring plate 5 height, observe monitoring consistent to ensure the light path of check point by camera 1, incident angle and reflection angle are 90 °; Light source 9 sends to first check point and detects light, and the wavelength utilizing spectroscopic probe head 3 acquisition testing point place is 800 nm, the reflectivity of tomato leaf corresponding to 853 nm, 1158 nm, 1520 nm, 1935 nm, 2012 nm, 2012 nm, 2250 nm and 2352 nm;
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other check point;
E () physics and chemistry value calculates: by gained three point reflection rates Input Control Element respectively, control module is according to multiple linear regression equations:
Y=15.336X1+20.256X2+32.114X3-26.458X4+358.542X5-489.668X6+256.334
X7+214.789X8-395.225X9+32.15, calculate the mda content of tomato leaf, in formula, Y is mda content in tomato leaf, X1 ~ X9 is respectively the reflectivity of wavelength 800 nm, 853 nm, 1158 nm, 1520 nm, 1935 nm, 2012 nm, 2012 nm, 2250 nm and the tomato corresponding to 2352 nm strain 17 blade alive, three check points obtain three mda content values, Y1, Y2 and Y3, Y1, Y2 and Y3 summation is made even and is mda content Y in tomato leaf.
Say from microcosmic, the active state of molecule of composition tomato leaf is subject to the energy excitation detecting light, produces different energy level transitions, absorbs and release energy, and forms corresponding with molecule reflectance spectrum and absorption spectrum.Different chemical bonds, as O-H, N-H, C-H etc., the rotation of chemical bond and stretching vibration, can absorb the light of specific wavelength in transition between energy level, and excite generation characteristic peak, and the intensity of foundation characteristic peak and wave number can judge the composition of material.
The vibration information of the chemical bonds such as N-H, C-N, N-H in the MDA of wavelength used and tomato leaf and related chemical constituents is relevant, and these information can reflect the number of mda content in pears tomato leaf.Such as 800 nm reflect the amido information of tomato leaf MDA, 853 nm reflect the aromatic hydrocarbons information of tomato leaf MDA, 1158 nm reflect the methine information of tomato leaf MDA, 1520 nm reflect the methyl information of tomato leaf MDA, 1935 nm reflect the primary amide information of tomato leaf MDA, 2012 nm reflect the swollen acid amides information of tomato leaf MDA, 2012 nm reflect the swollen acid amides information of tomato leaf MDA, and 2250 nm and 2352 nm reflect the group CH of tomato leaf MDA
3, CH
2with CH information.Application arithmetic of linearity regression carries out modeling analysis to above-mentioned characteristic wavelength, sets up the mda content of tomato leaf and the linear equation of characteristic wavelength.
The present invention the characteristic wavelength selected only for the quick detection of the mda content of tomato leaf, if need to measure other indexs, need again to select specific wavelength, by multiple linear regression, Modling model, calculates corresponding desired value.
Utilize the index of correlation of method establishment model provided by the invention in table 1, wherein, during modeling set representations Modling model, utilize the reflectivity of tomato leaf and existing method to test the tomato leaf mda content matching obtained and obtain model equation, forecast set represents, during prediction, after namely having set up model, according to model equation, the characteristic wavelength reflectivity of tomato leaf spectrum is substituted into the tomato leaf mda content that model equation calculates.
Table 1
| Data set | Number of samples | The coefficient of determination | Root-mean-square error | Slope |
| Modeling collection | 65 | 0.8602 | 13.48 | 0.8947 |
| Forecast set | 35 | 0.8513 | 14.57 | 0.8876 |
Embodiment 1 ~ 10
Utilize apparatus and method provided by the invention to test tomato leaf mda content, test result is in table 2.
Table 2
By table 2 data, can find out, it is close that the mda content of the tomato leaf utilizing the inventive method to record and existing method test the data obtained, and utilizes the present invention can the mda content of Fast Measurement tomato leaf, reliable results.
Claims (10)
1. a multi-angle In situ spectroscopic detects tomato leaf mda content device, include spectroscopic probe head, light source, it is characterized in that, also include all-directional rotation instrument and every electro-optical device, all-directional rotation instrument is by circular arc suspension plate, ring ring plate, electricity drives roller, supporting leg forms, circular arc suspension plate arc uniform arranges row's mounting hole, light source and spectroscopic probe head select one of them mounting hole to be connected in circular arc suspension plate according to crevice projection angle and incident angle respectively, circular arc suspension plate two head end is equipped with electricity and drives roller, ring ring plate is provided with the groove of opening upwards, electricity drives in roller embedding groove and advances along impression orbit, ring ring plate bottom surface is connected with passive supporting leg, during detection, tomato strain alive is in ring ring plate center, is entered wherein by all-directional rotation instrument every electro-optical device together with tested tomato strain cover alive.
2. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf mda content device, it is characterized in that: describedly include light shade cover, support every electro-optical device, light shade cover is made by every luminescent material, top has string holes, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, and string holes place is provided with sealing-plug.
3. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf mda content device, it is characterized in that: described circular arc suspension plate is also connected with camera.
4. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf mda content device, it is characterized in that: described electricity drives roller and is made up of roller and stepper motor, and stepper motor output shaft is connected with roller wheel shaft.
5. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf mda content device, it is characterized in that: be provided with the adjustable bolt regulating Circular Plate terrain clearance between described supporting leg and Circular Plate.
6. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf mda content device, it is characterized in that: described circular arc suspension plate semicircular in shape, and is marked with the scale mark representing suspension plate arc angle at sidewall.
7. multi-angle In situ spectroscopic according to claim 1 detects tomato leaf mda content device, it is characterized in that: the mounting hole number of described circular arc suspension plate is 18, and adjacent holes angle is 10 °.
8. multi-angle In situ spectroscopic according to claim 2 detects tomato leaf mda content device, it is characterized in that: describedly also include electric block every electro-optical device, light shade cover top is provided with suspension ring, and the suspension hook of electric block coordinates with suspension ring, and electric block is suspended on support.
9. multi-angle In situ spectroscopic according to claim 2 detects tomato leaf mda content device, it is characterized in that: described support is three-legged support.
10. multi-angle In situ spectroscopic detects a method for tomato leaf mda content, it is characterized in that: comprise the steps:
A () equipment prepares: erection bracket, lifting light shade cover, set up all-directional rotation instrument and level leveling, tomato strain alive is in the ring ring plate center of all-directional rotation instrument, spectroscopic probe head and light source are arranged in circular arc suspension plate respectively by mounting hole, cable and the optical fiber cable of spectroscopic probe head and light source pass from string holes, put down light shade cover;
B () spectrum blank is corrected: blank and the concordant placement of tomato strain blade height alive, carries out the rectification of spectrum blank;
C the angle of () Spectra of The Leaves adjusts sampling automatically: electricity drives roller and starts, circular arc suspension plate is driven to turn over certain angle together with spectroscopic probe head and light source, and coordinate adjustment ring ring plate height, consistent to ensure the light path of check point by camera observation monitoring, incident angle and reflection angle are 90 °; Light source sends to first check point and detects light, and the wavelength utilizing spectroscopic probe head acquisition testing point place is 800 nm, the reflectivity of tomato leaf corresponding to 853 nm, 1158 nm, 1520 nm, 1935 nm, 2012 nm, 2012 nm, 2250 nm and 2352 nm;
D the multi-point sampling of () Spectra of The Leaves: after having sampled, repeats step (c), completes the sampling of two other check point;
E () physics and chemistry value calculates: by gained three point reflection rates Input Control Element respectively, control module is according to multiple linear regression equations:
Y=15.336X1+20.256X2+32.114X3-26.458X4+358.542X5-489.668X6+256.334
X7+214.789X8-395.225X9+32.15, calculate the mda content of tomato leaf, in formula, Y is mda content in tomato leaf, X1 ~ X9 is respectively the reflectivity of wavelength 800 nm, 853 nm, 1158 nm, 1520 nm, 1935 nm, 2012 nm, 2012 nm, 2250 nm and the tomato corresponding to 2352 nm strain blade alive, three check points obtain three mda content values, Y1, Y2 and Y3, Y1, Y2 and Y3 summation is made even and is mda content Y in tomato leaf.
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| CN104568847A (en) * | 2014-12-21 | 2015-04-29 | 华东交通大学 | Device and method for detecting activity of catalase of tomato leaves by multi-angle in-situ spectrum |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502285A (en) * | 2014-12-21 | 2015-04-08 | 华东交通大学 | Device and method for detecting content of protein in tomato leaves by multi-angle in situ spectrum |
| CN104568847A (en) * | 2014-12-21 | 2015-04-29 | 华东交通大学 | Device and method for detecting activity of catalase of tomato leaves by multi-angle in-situ spectrum |
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