A kind of light channel structure of chromatography card detector
Technical field
The present invention relates to a kind of light channel structure, particularly relate to a kind of light channel structure of chromatography card detector.
Background technology
Immediately the application of (point-of-care testing) is detected for meeting POCT, the quick checkout equipment of chromatography has very high portable, small and exquisite demand, the light channel structure more complicated of the chromatography quick diagnosis test card detector of prior art, most two are adopted to excite and signal independently light path separately, main existence two shortcomings: (1) light path is long, cause whole detector volume excessive, be not easy to carry; (2) light path element is too much, debug difficulties, poor reliability, easy fault, and maintenance is inconvenient; (3) light path object distance is oversize, can only collect the signal light of very small divergence angle above detection zone; (4) exciting light light path can not be taken into account all vertical just to test card with flashlight light path.
Therefore, the direction becoming these those skilled in the art and make great efforts how is solved the problems of the technologies described above.
Summary of the invention
Object of the present invention is just to provide a kind of light channel structure of chromatography card detector, effectively can shorten light path, reduce light path element, reduce the volume of whole detector, ensure the reliability of whole detector, reduce the occurrence probability of fault, thus solve above-mentioned the deficiencies in the prior art part completely, especially be applied in the detection of time-resolved fluoroimmunoassay chromatography quick detection reagent card, and with on the chromatography card detector of scan mode detection, not only solve linear light sources to irradiate, in order to accept more weak fluorescence signal, light path is needed to provide plurality word aperture, collect the signal light at more Vernonia parishii Hook. F. angle as far as possible.
Object of the present invention is realized by following technical proposals:
A kind of light channel structure of chromatography card detector, comprise light source optical path and flashlight light path, exciting light sources produces excitation beam, excitation beam arrives detection zone through light splitting piece and cylindrical lens assembly successively and forms light source optical path, excitation beam focusing illumination produces flashlight in detection zone, flashlight is successively through cylindrical lens assembly, light splitting piece and photoelectric commutator form flashlight light path, photoelectric commutator is arranged on flashlight optical line terminal, for converting flashlight light intensity signal to electric signal, it is characterized in that: described light source optical path and flashlight light path are overlapping between cylindrical lens assembly and light splitting piece, described light splitting piece is totally reflected flashlight, and to excitation beam full impregnated mistake.
As preferably, described exciting light sources is directly over detection zone, and described light source optical path is a vertical straight line; Described photoelectric commutator is in side, detection zone, and flashlight light path is inverted "L" shaped.
As preferably, described exciting light sources is in side, detection zone, and light source optical path is inverted "L" shaped; Described photoelectric commutator is directly over detection zone, and flashlight light path is a vertical straight line.
Further, for detecting chromatography card with scan mode, chromatography card is motionless, light path system rectilinear movement or light path system motionless, chromatography card moves linearly.
Further, described light splitting piece can reflect the signal light wavelength light beam with certain incident angles, to excitation light source wavelength and the then transmission of parasitic light wavelength.
Further, described cylindrical lens assembly is one or two cylindrical lens, or a cylindrical mirror coordinates convex lens, or more than an one or 1 convex lens assembly unification light hurdle.
Further, described detection zone stimulated luminescence light source irradiation produces flashlight, and this flashlight is fluorescence, phosphorescence or scattered light.
Further, before flashlight optical line terminal photoelectric commutator, narrow band pass filter is set up.
Compared with prior art, beneficial effect of the present invention is: structure is simple, reasonable in design, effectively can shorten light path, reduce light path element, thus reduce the volume of whole detector, be easy to carry, ensure the reliability of whole detector, reduce the occurrence probability of fault.
Accompanying drawing explanation
Fig. 1 is the perspective view of the embodiment of the present invention one;
Fig. 2 is the side structure schematic diagram of the embodiment of the present invention one;
Fig. 3 is the Facad structure schematic diagram of the embodiment of the present invention one;
Fig. 4 is the perspective view of the embodiment of the present invention two;
Fig. 5 is the side structure schematic diagram of the embodiment of the present invention two.
Embodiment
Below in conjunction with specific embodiments and the drawings, the present invention is further illustrated.
Embodiment one
As shown in Figure 1 to Figure 3, a kind of light channel structure of chromatography card detector, comprises exciting light sources 1, light splitting piece 2, cylindrical lens assembly 3, narrow band pass filter 6, photoelectric commutator 4 and chromatography card detection zone 5.Described chromatography card detection zone 5 is the surveyed area that fluorescence immune chromatography examines on test card fast in the present embodiment.
Exciting light sources 1 is arranged on for generation of excitation beam directly over chromatography card detection zone 5, and vertically arrive chromatography card detection zone 5 through light splitting piece 2 and cylindrical lens assembly 3 successively forms light source optical path to excitation beam from top to bottom; Excitation beam focusing illumination produces flashlight in chromatography card detection zone 5, and flashlight is vertical after cylindrical lens assembly 3 focuses on from the bottom to top, is all-trans arrives photoelectric commutator 4 by light splitting piece 2 to horizontal direction.As preferably, set up the parasitic light in the further trap signal light light path of narrow band pass filter 6 in photoelectric commutator 4 front.Described light splitting piece 2 is 45 ° of angles with the optical axis of light source optical path, and it is totally reflected flashlight, and to excitation beam full impregnated mistake.
Described exciting light sources 1 can be LED light source, and flashlight is the fluorescence being excited to produce.Described photoelectric commutator 4 can be photomultiplier, photodiode or photoresistance.
Described light splitting piece 2 can be all-trans to the light within the scope of the signal light wavelength ± 100nm of certain incident angles, to excitation light source wavelength and parasitic light wavelength then full impregnated.
In the present embodiment, the piano convex cylindrical lens that described cylindrical lens assembly 3 is oppositely arranged by two convex surfaces forms, and in order to obtain larger digital aperture, the plane of below piano convex cylindrical lens is near detection zone 5, and spacing is less than 7mm.The crowning radius of two plano-convex cylindrical lens is 7mm, and the spacing between them is 2mm, and the focal length after two piano convex cylindrical lens combinations is 5-7mm, and collectable flashlight angle of divergence alpha reaches more than 60 °.Light splitting piece 2 is arranged on the position of 2mm above cylindrical lens assembly 3, owing to adopting the LED exciting light sources angle of divergence to be 6 °, exciting light sources 1 and flashlight photoelectric commutator 4 are all apart from reflecting piece 2 center 15mm, therefore, exciting with focusing efficiency of flashlight is higher, if the exciting light angle of divergence is less, then light path can be made shorter.
In the present embodiment, by using 2 piano convex cylindrical lens, on the one hand, excitation beam is formed line style hot spot in detection zone 5, vertical irradiation is in detection zone 5; On the other hand, the line style flashlight that the 2 π solid angles of long strip type of being excited to produce are dispersed is focused on, although the flashlight dispersed along piano convex cylindrical lens long side direction can not be focused, but all can be focused along the signal light in short the direction 0-60 ° angle of divergence, luminous energy receiving efficiency is far away higher than employing convex lens focus.
Described cylindrical lens assembly 3 can also be that a cylindrical mirror coordinates convex lens, or more than an one or 1 convex lens assembly unification light hurdle is formed.
Described chromatography card detection zone 5 is irradiated through exciting light sources 1 and is produced flashlight, and this flashlight can be fluorescence, phosphorescence or scattered light.
In order to shorten light path further, simplify structure, the length and width of the hot spot that described exciting light sources 1 projects on cylindrical lens assembly 3 are less than 8mm × 8mm, the angle of divergence is less than ± and 5 °, the angle of divergence of described flashlight after cylindrical lens assembly 3 be less than ± and 5 °, the focal length of described cylindrical lens assembly 3 is less than or equal to 10mm.
Feature of the present invention is, described light source optical path and flashlight light path are overlapping between cylindrical lens assembly 3 and light splitting piece 2, and described light source optical path is straight line from top to bottom, flashlight light path is inverted "L" shaped circuit, light source optical path and the equal vertical detection region of flashlight light path.Relative to the light channel structure of prior art, can excite more expeditiously and Received signal strength luminous energy, its light path is shorter, is conducive to the volume reducing whole detector, is easy to carry.On the other hand, whole light channel structure is only made up of exciting light sources 1, light splitting piece 2, cylindrical lens assembly 3, photoelectric commutator 4 and chromatography card detection zone 5, light path element is less compared to prior art, therefore, without the need to light path calibration, be conducive to the occurrence probability reducing fault, improve reliability, and be also conducive to the volume reducing whole detector.In order to improve the signal to noise ratio (S/N ratio) of flashlight, set up a narrow band pass filter 6 in photoelectric commutator 4 front, the signal light wavelength that further purification receiving receives.
Embodiment two
As shown in Figure 4 and Figure 5, the difference of the present embodiment and embodiment one is, described exciting light sources 1 is in side, chromatography card detection zone 5, and light source optical path is inverted "L" shaped; Described photoelectric commutator 4 is directly over chromatography card detection zone 5, and flashlight light path is a vertical straight line.
Exciting light sources 1 is arranged on side, chromatography card detection zone 5 for generation of excitation beam, and excitation beam level is all-trans to vertical direction through light splitting piece 2, and arrives chromatography card detection zone 5 and form light source optical path after cylindrical lens assembly 3 focuses on; Excitation beam focusing illumination produces flashlight in chromatography card detection zone 5, and flashlight is vertical after cylindrical lens assembly 3 focuses on from the bottom to top, after full impregnated crosses light splitting piece 2, after the parasitic light in narrow band pass filter 6 trap signal light light path, arrives photoelectric commutator 4.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.