CN104450571B - A kind of bacillus thuringiensis bacterial strain of efficient degradation fly-maggot protein - Google Patents
A kind of bacillus thuringiensis bacterial strain of efficient degradation fly-maggot protein Download PDFInfo
- Publication number
- CN104450571B CN104450571B CN201410729336.1A CN201410729336A CN104450571B CN 104450571 B CN104450571 B CN 104450571B CN 201410729336 A CN201410729336 A CN 201410729336A CN 104450571 B CN104450571 B CN 104450571B
- Authority
- CN
- China
- Prior art keywords
- fly
- maggot protein
- bacillus thuringiensis
- bacterial strain
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to a kind of bacillus thuringiensis bacterial strain KF228911 of efficient degradation fly-maggot protein, belong to field of agricultural microbial technology.The strain classification is named as serum anomaly bacillus thuringiensis (Bacillus thuringiensis serovar thuringiensis), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on October 17th, 2014, culture presevation number is CGMCC NO.9789.By inoculation in seed culture medium, under the conditions of 30 DEG C, cultivated 28 hours with 150r/min；Obtain KF228911 liquid cultures.The bacterial strain produces hydrolysis on Flyblow albumen powder culture medium, fly-maggot protein of being degraded in fly-maggot protein fermentation medium, produces soluble Amino acid content and reaches 1.744mg/mL.The present invention is the method degraded fly-maggot protein using microbial fermentation, it is adaptable to the production of high-quality protein feed.Available for the exploitation of fly-maggot protein resource, fly-maggot protein is particularly changed into feed, its nutritive value is improved.
Description
Technical field
The present invention relates to a kind of fly-maggot protein efficient degrading bacteria, belong to field of agricultural microbial technology, be to utilize microorganism
The method degraded fly-maggot protein of fermentation, it is adaptable to the production of high-quality protein feed.
Background technology
In recent years, with the fast development of China's feed industry, there is lack of raw materials that problem is seriously limited for protein feeds
The development of Zhi Liao China feed industry.At present, China is mainly by imported fish meal the problem of deficiency to solve protein resource, institute
Turn into the hot subject of scientific research to develop new protein resource.Soybean is the protein raw materials of high-quality, but its higher valency
Lattice will undoubtedly limit the development of feed industry.Therefore, the protein feed raw material of new safety is developed, seeks and promoted as early as possible
It is current top priority.Housefly is a kind of most commonly seen insect in area from all parts of the world, and its quantity is huge, and fertility is strong, numerous
Grow speed fast.Fly maggot is the Larva Morpho. Logy of housefly, contains a variety of aliphatic acid, amino acid, vitamin and mineral matter.In China's fly maggot
It can be bred, with higher protein content, can be developed.Flyblow albumen powder has been made at present.Fly
Protein content is about 60.53% in maggot, and amino acid classes are complete, and 8 containing needed by human body kinds of essential amino acids and group ammonia
Acid, its essential amino acids content accounts for the 42.14% of total amino acid content, must well beyond the FAO/WHO good proteins advised
Amino acid is needed to account for the standard of total amino acid content 40%.Fly-maggot protein is more not a halfpenny the worse compared with other high-quality animal proteins, its
Nutritive value is even higher than other animal proteins, therefore it is even more a kind of protein resource of high-quality.But fly-maggot protein is such as
, but there is the problem of fly-maggot protein nutritional utilization is low in fruit Direct-fed, and fly-maggot protein matter is in hydrolysis oligopeptides, polypeptide and ammonia
After the mixture of base acid, the nutritive value of feed can be improved, is conducive to the absorption of animal；It is of course possible to be dropped using enzyme process
Solution, but cost is high, and also purchased prolease activity is low, and hydrolysis degree is small, if using fly-maggot protein degradation bacteria strains, can both save
Cost, can improve hydrolysis degree again.Current studies in China is less, is the developing direction of fly-maggot protein fodder.
The content of the invention
It is an object of the invention to provide a kind of fly-maggot protein efficient degrading bacterial strain, for the degraded of fly-maggot protein, so as to open
The useful protein resource that to send out a kind of new, solves the problem of current fly-maggot protein nutritional utilization is low.
The present invention provides a kind of bacillus thuringiensis bacterial strain of efficient degradation fly-maggot protein, and strain classification is named as serum
Anomaly bacillus thuringiensis (Bacillus thuringiensis serovar thuringiensis), in October, 2014
China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved within 17th, culture presevation number is CGMCC
NO.9789.Main Biological is that colonial morphology is circular, milky, and surface wettability is typical ne ar, through leather
Blue Albert'stain Albert decoration method and spore staining method identification, the bacterial strain is Gram-negative bacillus.Blood is accredited as through gene sequencing
Clear anomaly bacillus thuringiensis.Bacterial strain Sep-7S ribosomal RNA gene accession number is KF228911.
Fly-maggot protein degradation bacteria strains can grow generation hydrolysis, its culture medium prescription on Flyblow albumen powder culture medium
For：Flyblow albumen powder containing 2.0g in 100mL distilled water, 0.5g sodium chloride, 2.0g agar powders are dissolved by heating, and adjust pH value 7.0,
121 DEG C of autoclaving 30min.Described Flyblow albumen powder is after fly maggot is dried, to carry out crushing and form, cross 100 mesh sieves.
Above-mentioned bacterial strains KF228911 expansion cultural method is：
(1) formula of seed culture medium is：Contain in 1L distilled water：3g beef extracts, 10g peptones, 5g sodium chloride is heated molten
Solution, 7.0,121 DEG C of autoclaving 30min of regulation pH value.
(2) described bacterial strain KF228911 is inoculated in the conical flask of the 250mL equipped with 100mL seed culture mediums, temperature
Under the conditions of 30 DEG C of degree, with 150r/min rotating speed culture 24 hours on shaking table, bacteria suspension is obtained, bacterium solution quantity is 4 ×
1010CFU/ml。
Fly-maggot protein degradation bacteria fermentative medium formula is：Contain in 1L distilled water：20g Flyblow albumen powders, 20g glucose,
5g sodium chloride, 7.0,121 DEG C of autoclaving 30min of regulation pH value.
It is inoculated with fly-maggot protein degradation bacteria KF228911 bacteria suspensions with 5% inoculum concentration, after fermentation, can be to fly
Maggot protein is degraded, 30 DEG C of cultivation temperature, and 150r/min is cultivated 48 hours, to zymotic fluid 4000r/min after culture is completed
Centrifugation 20 minutes is carried out, supernatant is collected, soluble Amino acid content can be produced for 1.744mg/mL.
Beneficial effect
The invention provides the bacillus thuringiensis bacterial strain KF228911 of efficient degradation fly-maggot protein.Fly-maggot protein is degraded
Bacterium can grow on Flyblow albumen powder culture medium, produce hydrolysis.There is provided fly-maggot protein degradation bacteria KF228911 expansion
Big cultural method.With fly-maggot protein degradation bacteria strains culture fly-maggot protein fermentation medium, fly-maggot protein degraded is produced in zymotic fluid
Amino acid content be 1.744mg/mL.It is solvable that fly-maggot protein fodder such as inoculation fly-maggot protein degradation bacteria KF228911 can improve its
The content of acidic amino acid, improves fly-maggot protein as the nutritive value of feed, with vast potential for future development.
The bacillus thuringiensis bacterial strain of the energy efficient degradation fly-maggot protein of the present invention, its entitled serum anomaly Su Yun
Golden bacillus (Bacillus thuringiensis serovar thuringiensis) KF228911, is preserved in China micro-
Biological inoculum preservation administration committee common micro-organisms center (CGMCC), deposit number is CGMCC NO.9789.Depositary institution
Address is located at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, and preservation date is 2014 10
The moon 17.
Embodiment
The high efficient strain of embodiment 1 is separated
Take fly maggot dead in natural environment to drive 1 gram of body sample, add 100mL sterile salines, vibrate 10 minutes,
5 minutes are stood, is coated on after 10 times of dilutions of supernatant on screening flat board culture medium, 37 DEG C of growths；Fly-maggot protein degradation bacteria strains are sieved
Select culture medium composition be：2.0g Flyblow albumen powders, 0.5g sodium chloride, 2.0g agar powders, water 100mL, pH value is 7.0,121 DEG C and gone out
Bacterium 30min.
Culture medium compound method of the present invention is as follows：
Flyblow albumen powder 2.0g accurately is weighed, add water 100mL, heating makes protein dissolution, adds 0.5g sodium chloride, 2.0g fine jades
Cosmetics, adjusts pH value, 121 DEG C of sterilizing 30min.
Fly-maggot protein degradation bacteria strains screening embodiment is as follows：
1. primary dcreening operation：Liquid is sampled, after the suitably dilution of conventional 10 times of dilution methods, takes 0.1mL to be coated on fly-maggot protein degraded
On bacterial strain screening culture medium flat plate, in 37 DEG C of 24~48h of constant incubator culture.Measure single bacterium colony hydrolysis transparent circle and bacterium colony is straight
Footpath, primary dcreening operation is carried out according to transparent loop diameter and colony diameter ratio (H/C) size, is therefrom chosen 12 plants of larger bacterial strains of ratio and is entered
Row is preserved and shake flask fermentation screening.
Beef extract-peptone inclined-plane Storaged media：In 100mL water, beef extract 0.3g, peptone 1.0g, NaCl are added
0.5g, agar 2.0g, adjust pH value 7.0.
Ferment minimal medium：In 100mL water, wheat bran 3.0g, peptone 1.0g, NaCl 0.5g, pH are naturally, 121 DEG C go out
Bacterium 30min.
Shake flask fermentation：It is inoculated into oese picking strain in fermentation minimal medium, 36 DEG C of fermentation temperature, shaking speed
For 180r/min, fermented and cultured 48h, enzyme activity is determined, the maximum bacterial strain of producing enzyme vigor is chosen；
(1) enzyme activity determination method：
The drafting of the tyrosine standard curve of table 1：
18 test tubes are taken to be divided into 6 groups, it is every group 3, parallel and number, it is separately added into standard tyrosine, deionization by table 1
Water, 0.4mol/L sodium carbonate and Folin-Phenol reagent, after mixing, are put into 40 DEG C of water-bath insulation 20min, then in 721 type light splitting
Colorimetric estimation (wavelength 660nm) is carried out on photometer, blank control is done with deionization.With OD660It is worth for abscissa, tyrosine amount
(μm ol) is ordinate, obtains equation of linear regression；Y=109.34 × OD660- 1.9026, linearly dependent coefficient R2=0.9983
(2) measure of sample enzyme activity
4 centrifuge tube numberings (No. 0 is blank control) are taken, crude enzyme liquid 1mL are separately added into, No. 0 pipe adds 0.4mol/ immediately
L TCA solution 2mL, inactivate enzyme, and another 3 samples add 1% casein solution 1mL, rapid to mix, and are immediately placed in 40 DEG C of perseverances
After the accurate timing of tepidarium, insulation 10min, 0.4mol/L TCA solution 2mL, terminating reaction, while to No. 0 blank are added immediately
1% casein solution 1mL is added in pipe, is shaken up, continues to be placed in water-bath and is incubated 20min, taking-up is centrifuged off remaining casein
And zymoprotein sediment, each pipe centrifugate 1mL is then taken, moves into respectively in another 4 10mL scale test tubes, adds 0.4mol/L
The sodium carbonate liquor 5mL and Folin-Phenol reagent 1mL of oneself dilution, shakes up, and after insulation colour developing 20min, carries out colorimetric estimation (wavelength
660nm).Tyrosine growing amount A is calculated by tyrosine equation of linear regression.
Prolease activity is calculated：Prolease activity：A×4.0×N/(l0×1.0).
In formula：A represents the tyrosine growing amount obtained by equation of linear regression；4.0 represent reaction solution cumulative volume (mL)；N
Represent the extension rate of enzyme liquid；10 represent the reaction time (min)；1.0 represent enzyme liquid volume (mL)；Proteinase activity is defined：
Under certain pH value (pH7.0) and 40 DEG C of temperature conditionss, the enzyme amount definition of 1 μ g tyrosine is produced per min protease hydrolytics casein
For a protease activity unit of force.
2. secondary screening：Bacterial strain selected after primary dcreening operation is pressed into the step after primary dcreening operation, is cultivated again and secondary screening, utilizes fly maggot
Protein degradation bacterial strain screening culture medium, secondary screening is carried out by the same method of primary dcreening operation.
3. preservation：The high bacterial strain of producing enzyme vigor is subjected to preservation using beef extract-peptone inclined-plane Storaged media.
4. strain idenfication
Colonial morphology, color, feature are observed in (1) 10 times of dilution method culture；
(2) by Gram's stain, in oily Microscopic observation, identification Gram-negative or the positive；
(3) spore staining method identifies the species of bacterial strain, if be bacillus；
Using the method for the present invention, the bacterial strain for obtaining one plant of energy efficient degradation fly-maggot protein, the strain classification can be screened
Serum anomaly bacillus thuringiensis (Bacillus thuringiensis serovar thuringiensis) is named as,
On October 17th, 2014 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and culture presevation number is
CGMCC NO.9789.Main Biological is that colonial morphology is circular, milky, and surface wettability is typical bacterium shape
State, identifies, the bacterial strain is Gram-negative bacillus through Gram's stain and spore staining method.The DNA's of the bacterial strain
Genbak, similitude highest is：Bacillus thuringiensis serovar thuringiensis, the number of logging in is：
KF228911
The specific zymologic property research of fly-maggot protein digestive enzyme of embodiment 2 is as follows：
The source of enzyme：Fly-maggot protein degradation bacteria strains be inoculated in fermentation minimal medium (wheat bran 3.0%, peptone 1.0%,
NaCl0.5%, pH are naturally, 121 DEG C of sterilizing 30min) in, 36 DEG C, rotating speed 180r/min is cultivated 48 hours, nutrient solution 4000r/
Min centrifuges 10min, and supernatant is fly-maggot protein degraded enzyme liquid.
(1) optimum temperature of enzyme：Different temperatures (35,40,45,50,55,60 DEG C) under determine the vigor of digestive enzyme, enzyme
Temperature during vigor highest is the optimum temperature of enzyme
(2) stability of the enzyme to temperature：Digestive enzyme different temperatures (35,40,45,50,55,60 DEG C) under be incubated 1h, then
The vigor of enzyme is determined, so as to know that enzyme is stable at a temperature of which.
(3) optimum pH of enzyme：The vigor of digestive enzyme is determined under different pH (5.5,6.0,6.5,7.0,7.5,8.0),
PH during enzyme activity highest is the optimum pH of enzyme；
(4) stability of the enzyme to pH：Digestive enzyme keeps 1h under different pH (5.5,6.0,6.5,7.0,7.5,8.0), then
The vigor of enzyme is determined, so as to know enzyme is stable under which pH value.
Interpretation of result
Using the method for the present invention, fly-maggot protein efficient degradation enzyme zymologic property can be obtained as follows：The optimal reaction of enzyme
Temperature is 50 DEG C, and after 35 DEG C are incubated 1 hour, enzyme activity still retains 76.9%, and more than 35 DEG C enzyme activity decline rapidly,；Enzyme it is most suitable
PH value be 7.5), after being kept for 1 hour during pH value is 6.0,6.5,7.0 buffer solution, the phenomenon that the vigor of enzyme is all improved.
The fly-maggot protein of embodiment 3 biodegradation effect：
Culture medium prescription：Glucose 2.0g, fly-maggot protein 2.0g, NaCl 0.5g；Water 100mL；
1) each component is weighed respectively according to culture medium composition, dissolved using running water；
2) culture medium configured is fitted into triangular flask, stirred and evenly mixed, 121 DEG C sterilize 30 minutes, cooling；
3) fly-maggot protein degradation bacteria is inoculated with, 48h is cultivated；
4) supernatant determines amino acid growing amount after nutrient solution 4000r/min, to determine its palliating degradation degree.
The measure of amino acid
The drafting of leucine standard curve
100 μ g/mL standard leucine sample liquids are prepared, 6 test tube numberings is taken, standard leucine is separately added into, without ammonia by table 2
Distilled water, ninhydrin and 0.1% ascorbic acid, are put into boiling water bath after mixing and heat 15min, often shake, make heating
When the red gradually oxidation by air that is formed and when fading to blue purple, arrive 20mL with 60% ethanol constant volume, mixing, Ran Hou
O.D is determined under 570nm wavelength570Value.
The drafting of the leucine standard curve of table 2
| Guan Hao | 0 | 1 | 2 | 3 | 4 | 5 |
| Standard leucine (mL) | 0 | 0.2 | 0.4 | 0.6 | 0.8 | 1.0 |
| Without ammonia distilled water (mL) | 2.0 | 1.8 | 1.6 | 1.4 | 1.2 | 1.0 |
| Ninhydrin (mL) | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 | 3.0 |
| 0.1%Vc | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| O.D570Value | ||||||
| Leucine amount y (μ g) | 20 | 40 | 60 | 80 | 100 |
With O.D570For abscissa (x), leucine amount (y) is ordinate, draws standard curve, obtains its equation of linear regression：
Y=220.51-0.1994, R2=0.9912.
Centrifuge tube four is taken, No. 0 takes the culture medium 2.0mL for dissolving by heating but not being inoculated with degraded as blank control, another three
2.0mL culture supernatants are drawn, each TCA aqueous solution 2.0mL for adding 0.4mol/L mix, stand 20min, Ran Hou
10min is centrifuged under 4000r/min, albumen precipitation thing is removed, then takes each pipe centrifugate 0.1mL, 4 20mL scales are moved into respectively
In test tube (reference numeral), no ammonia distilled water 1.9mL is added, same Specification Curve of Increasing is operated below, blank pair is used as with No. 0
According to the colorimetric estimation O.D under 570nm wavelength570, amino acid growing amount (y) is calculated by equation of linear regression.
Interpretation of result
Using the culture medium and its fermentation process of the present invention, zymotic fluid produces amino acid content 1.744mg/mL.Therefore raise
The content of soluble amino acid in fly-maggot protein can be improved as being inoculated with fly-maggot protein degradation bacteria in material, fly-maggot protein is improved and is used as feeding
The nutritive value of material, it can also be used to the industrialized production of amino acid.
Claims (4)
1. the application of soluble Amino acid content of the bacillus thuringiensis bacterial strain in fly-maggot protein fodder is improved, its feature exists
In：The entitled serum anomaly bacillus thuringiensis of the bacterial strain (Bacillus thuringiensis serovar thuringiensis) KF228911, it is common that on October 17th, 2014 is preserved in China Committee for Culture Collection of Microorganisms
Microorganism center, culture presevation number is CGMCC NO.9789.
2. the application described in claim 1, wherein the bacillus thuringiensis suspension that will be enlarged by is inoculated in 5% inoculum concentration
Under the conditions of fermentation medium, 30 DEG C of temperature, 150 r/min are cultivated 48 hours, and it is 1.744mg/ to produce soluble Amino acid content
mL；Fermentative medium formula is：Contain 20g Flyblow albumen powders in 1L water, 20g glucose, 5g sodium chloride adjusts pH value 7.0,
121 DEG C of min of autoclaving 30, centrifugation 20 minutes is carried out after culture is completed to the r/min of nutrient solution 4000, collects supernatant.
3. the expansion cultural method of the application described in claim 2, wherein bacillus thuringiensis bacterial strain is：
(1), the configuration of seed culture medium：Contain 3g beef extracts in 1L water, 10g peptones, 5g sodium chloride adjusts pH value 7.0,
121 DEG C of min of autoclaving 30；
(2), bacterial strain KF228911 described in claim 1 is inoculated in the conical flask of 250 mL equipped with 100 mL seed culture mediums
In, under the conditions of 30 DEG C of temperature, with 150 r/min rotating speed culture 24 hours on shaking table.
4. the application described in claim 1 or 2 or 3, soluble Amino acid content wherein can be produced in fly-maggot protein fodder is
1.744mg/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410729336.1A CN104450571B (en) | 2014-12-04 | 2014-12-04 | A kind of bacillus thuringiensis bacterial strain of efficient degradation fly-maggot protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410729336.1A CN104450571B (en) | 2014-12-04 | 2014-12-04 | A kind of bacillus thuringiensis bacterial strain of efficient degradation fly-maggot protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104450571A CN104450571A (en) | 2015-03-25 |
| CN104450571B true CN104450571B (en) | 2017-08-25 |
Family
ID=52897330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410729336.1A Expired - Fee Related CN104450571B (en) | 2014-12-04 | 2014-12-04 | A kind of bacillus thuringiensis bacterial strain of efficient degradation fly-maggot protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104450571B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533136B (en) * | 2015-12-25 | 2019-11-05 | 广东鑫肽生物科技股份有限公司 | A kind of preparation method and worm ferment of novel fermentation feed |
| CN106190904B (en) * | 2016-07-17 | 2018-06-19 | 湖南农业大学 | Fly-maggot protein biology solid-state degradation prepares ammonia nitrogen feed process |
| GB2561348B (en) * | 2017-04-07 | 2020-01-15 | Entomics Biosystems Ltd | Process for converting invertebrates into feedstock |
| CN112608871B (en) * | 2021-01-12 | 2022-11-01 | 江南大学 | Method for producing probiotic active substances by high-density fermentation of bacillus thuringiensis |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372678A (en) * | 2008-10-22 | 2009-02-25 | 南京农业大学 | Keratin degrading bacteria NJY1 |
| CN103820352A (en) * | 2013-06-07 | 2014-05-28 | 华南农业大学 | Bacillus cereus YSQ08 and application thereof |
-
2014
- 2014-12-04 CN CN201410729336.1A patent/CN104450571B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372678A (en) * | 2008-10-22 | 2009-02-25 | 南京农业大学 | Keratin degrading bacteria NJY1 |
| CN103820352A (en) * | 2013-06-07 | 2014-05-28 | 华南农业大学 | Bacillus cereus YSQ08 and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 蝇蛆的营养价值及在水产养殖方面的应用;朱绍辉;《饲料研究》;20121231;58-61 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104450571A (en) | 2015-03-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106167782B (en) | The bacillus of efficient degradation dregs of beans antigen protein and its method for fermented bean dregs | |
| CN104694424B (en) | One plant of Bacillus amyloliquefaciens strain for being isolated from producing protease in fermented soya bean | |
| CN102154167A (en) | Mycoplasma hyopneumoniae culture medium and preparation method thereof | |
| CN106282044B (en) | A kind of preparation method of Hyphomicrobium and pyrroloquinoline quinone | |
| CN104450571B (en) | A kind of bacillus thuringiensis bacterial strain of efficient degradation fly-maggot protein | |
| CN110317748B (en) | Streptomyces strain and application thereof in feather degradation | |
| CN103627662A (en) | Peanut bradyrhizobium sp. and application thereof | |
| CN104087511A (en) | Mucor racemosus strain and its application | |
| CN110819572B (en) | Bacillusmycoides | |
| CN103820352A (en) | Bacillus cereus YSQ08 and application thereof | |
| CN103898016A (en) | High-yield lactic acid bacterium and method for preparing calcium lactate by fermenting eggshells with same | |
| CN106434457B (en) | One plant of alkali protease superior strain and its produced alkali protease | |
| CN105400729A (en) | Antibacterial bacillus subtilis strain producing xylanase | |
| CN115261262B (en) | Lactobacillus plantarum HSF-LAB-1303 and application thereof | |
| CN105296392B (en) | The fermentation process for preventing and treating Cruciferae clubroot bacillus amyloliquefaciens Bam22 | |
| CN105400717A (en) | Bacterial strain HBRM-16 capable of promoting growth of roots of rubber tree and application of bacterial strain HBRM-16 | |
| CN104894026B (en) | The cultural method of bafillus natto and its application | |
| CN104745554B (en) | Bacillus produces the fermentation medium and fermentation process of protease and gemma | |
| CN107557311B (en) | Lactobacillus acidophilus and application thereof in fermentation production of antibacterial polypeptide | |
| CN107384879B (en) | A kind of fermentation manufacturing technique of recombinant yeast pichia pastoris bacterium production liver detoxification enzyme | |
| CN114350553B (en) | Bacillus amyloliquefaciens capable of producing protease at high yield and application thereof | |
| CN107641602B (en) | Candida utilis and application thereof in protein production through fermentation | |
| CN112300953A (en) | Bacillus subtilis and application thereof in fermentation production of adenosine deaminase | |
| CN110229763A (en) | One plant of flocculant produces bacterium and its in the cultivation of prawn biological flocculation and dye decolored middle application | |
| CN105349465B (en) | A kind of intertidal zone Exiguobacterium sp and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170825 Termination date: 20191204 |