CN104450509B - Spirulina cells broken wall shear - Google Patents
Spirulina cells broken wall shear Download PDFInfo
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- CN104450509B CN104450509B CN201410511684.1A CN201410511684A CN104450509B CN 104450509 B CN104450509 B CN 104450509B CN 201410511684 A CN201410511684 A CN 201410511684A CN 104450509 B CN104450509 B CN 104450509B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/08—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers
- B02C18/12—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers with drive arranged below container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
- B02C18/18—Knives; Mountings thereof
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- Life Sciences & Earth Sciences (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
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- Sustainable Development (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Crushing And Pulverization Processes (AREA)
Abstract
A kind of spirulina cells broken wall shear, comprise and shear chamber, its top is fixed with upper cover plectane, the chassis in shearing chamber is sealed and installed with rotor by drive shaft and shears impeller, drive shaft passes the chassis in shearing chamber and connects motor, and drive shaft is tightly connected with the chassis institute perforate of shearing chamber; The circumferential direction uniform intervals shearing chassis, chamber is provided with multiple shell knife, shell knife head is provided with symmetrical knife edge face, blade is formed on top, two knife edge faces, be separately installed with stator gear piece between the knife edge face of adjacent two shell knifes, the blade of shell knife, the blade of stator gear piece and rotor are sheared between impeller and are left gap; Upper cover plectane inner face clamping backgauge annulus, abut with shell knife and stator gear piece bottom it, the inner face of upper cover plectane is also fixed with guide annulus by fastening piece, and guide annulus is positioned at the top that impeller sheared by rotor; Install feed cavity in the middle part of upper cover plectane, the sidewall bottom shearing chamber has discharge port.Shearing-crushing is effective.
Description
Technical field
The present invention relates to biomass cells means for breaking walls technical field, especially a kind of spirulina cells broken wall shear.
Background technology
Cell wall breaking technology is as a kind of high-tech means, and its application is very extensive.Except virus, all living things is by cellularity, and according to the difference of intracellular nucleic structure differentiation degree, cell can be divided into prokaryotic cell prokaryocyte and the large type of eukaryotic cell two.Cell walls (cellwall) is the skin of cell, and in the outside of cytolemma, the thickness of cell walls is often different because tissue, function are different.Plant, fungi, algae and prokaryotic organism all have cell walls, and zooblast does not have cell walls.The short texture of cell walls own, the external world enters in cell by cell walls.
Spirulina is aquatic algae such as a class low grade, and its outward appearance is blue-greenish colour, and microscopic examination is spirrillum, thus obtains its name.Spirulina is a kind of nutritive food of alkalescence, and it contains the nutritive ingredient of various needed by human, and ratio of components is comparatively even, and wherein protein content is that in current known all natural phant, content is the highest, accounts for 70% of dry weight.Phycocyanins, C-is isolated a kind of dark blue powder from spirulina, and color and luster is attractive in appearance.It is a kind of protein, is again a kind of fabulous natural food colour, is again good protective foods simultaneously.The cell walls of spirulina hinders effective extraction of Phycocyanins, C-, effectively will extract the Phycocyanins, C-matter in spirulina cells, just must carry out cell wall breaking to spirulina cells.
Existing cell wall breaking technology comprises supersonic wave wall breaking, polishing broken wall, multigelation method broken wall, melts bacterium enzyme broken wall, shearing method.Supersonic wave wall breaking method is limited to hyperacoustic penetration coefficient, can only be applied in the spirulina cells broken wall occasion of experiment level.The instrument of polishing broken wall method steel alloy, easily have the residual of the composition of metal, and the process of grinding can produce high temperature Phycocyanins, C-matter.Thawing wanted by multigelation method broken wall, broken wall inefficiency, and production capacity is low, and power consumption is high.Melt bacterium enzyme broken wall method because needs use specific enzyme, because this increasing cost.In spirulina cells technology for broken wall, spirulina cells should broken certain fineness, but particle size after cracking can not be made too thin, increases difficulty with the follow-up centrifugal separating cell wall of exempt from customs examination.Existing ultra-fine shearing device adopts tooth-shape structure, after using for a long time, is often discarded by whole gear ring, the gear ring again more renewed, and used gear ring can not manufacture again and reuse, causes maintenance cost high.Also lack the cell wall breaking shearing equipment that Spirulina phycocyanin is extracted in industrialization at present.Therefore a kind of efficient, energy-conservation, green, reusable cell wall breaking equipment for spirulina slurry is developed very urgent.
Summary of the invention
The applicant is for the shortcoming in above-mentioned existing production technology, a kind of spirulina cells broken wall shear rational in infrastructure is provided, thus effectively solve that spirulina cells broken wall inefficiency, power consumption are high, secondary pollution problems defect, namely reach good crushing effect, ensure again maintainability and the reusability of broken wall shearing device.
The technical solution adopted in the present invention is as follows:
A kind of spirulina cells broken wall shear, comprise and shear chamber, the top in described shearing chamber has been bolted upper cover plectane, the chassis in described shearing chamber is sealed and installed with rotor by drive shaft and shears impeller, described drive shaft passes the chassis in shearing chamber and connects motor, and described drive shaft is tightly connected with the chassis institute perforate of shearing chamber; The circumferential direction uniform intervals on chassis, described shearing chamber is provided with multiple shell knife, described shell knife head is provided with symmetrical knife edge face, blade is formed on top, two knife edge faces, be separately installed with stator gear piece between the knife edge face of adjacent two shell knifes, the blade of described shell knife, the blade of stator gear piece and rotor are sheared between impeller and are left gap; The inner face of described upper cover plectane is connected with backgauge annulus, the bottom of described backgauge annulus abuts with shell knife and stator gear piece, the inner face of described upper cover plectane is also fixed with guide annulus by fastening piece, and described guide annulus is positioned at the top that impeller sheared by rotor; Install feed cavity in the middle part of described upper cover plectane, the sidewall bottom in described shearing chamber is provided with discharge port.
Further improvement as technique scheme:
The structure that impeller sheared by described rotor is: comprise annulus cover, described annulus cover middle part is tightly connected with drive shaft, on the outer circumference surface of described annulus cover, uniform intervals is provided with multiple blade, and described blade has angling hole, described angling hole in the middle part of blade to the setting from high to low of head; The head being positioned at blade is provided with inclined-plane;
The structure of described shell knife is: described shell knife becomes rectangular structure, has slotted hole in the middle part of described shell knife;
The structure of described stator gear piece is: the structure in echelon of described stator gear piece, and the bottom of described stator gear piece is formed with broached-tooth design;
The knife edge face of described shell knife both sides is parallel with the two sides of stator gear piece, and the gap between the knife edge face of described shell knife both sides and stator gear piece side is 3mm-5mm;
The blade of described shell knife, the blade of stator gear piece and the rotor gap of shearing between impeller are 0.5mm-1mm;
The knife edge face of described shell knife and the angle a of blade horizontal direction are 15 ° ~ 45 °;
The knife edge face of described shell knife and the angle a of blade horizontal direction are 30 °.
Beneficial effect of the present invention is as follows:
Compact construction of the present invention, rationally, easy to operate, utilize the blade of the blade of shell knife, stator gear piece and rotor to shear high speed shear between impeller, multi-blade fly-cutting improves the cutting effect to spirulina, the fineness of guarantee crushing material; In addition, shell knife and stator gear piece adopt adjustable and removably, when blade is worn, can dismantle lower shell knife and stator gear piece, and grinding blade is to sharp state again; Then again shell knife and stator gear piece are arranged on and shear bottom chamber, and do minute movement adjustment towards shearing direction, the center of circle, chamber, ensure that shear gap is adjustable, the adjustment in gap is conducive to spirulina cells and obtains effective shearing-crushing.
Notable feature of the present invention is shell knife, stator gear piece is detachable, adjustable, can manufacture again, add the reusability of this shearing device key components and parts, simultaneously also can according to the requirement of particle size after cracking, shell knife, stator gear piece and rotor is regulated to shear the gap of impeller, gap is less, and crushing effect is better.
Shell knife of the present invention adopts split-type structural, adopts multiple shell knife to surround circular configuration, and is assembled in the bottom of shearing chamber, long use, when shell knife weares and teares, single shell knife can be dismantled, again correctly install again after ground finish.
Stator gear piece of the present invention, between the knife edge face being arranged on adjacent two shell knifes, adopts split-type structural equally, can conveniently install and remove, long-term use, again ground finish blade, more correctly install.In addition, the design of the gear teeth on stator gear piece, the radial cuts power between impeller sheared by raising stator gear piece and rotor, ensures shear effect.
Every sheet blade of rotor shearing impeller of the present invention has angling hole, after material enters from feed cavity, the overwhelming majority material directly by angling hole be jet-like be directly thrown toward rotor shearing impeller blade place, ensure that material obtains effective shearing-crushing in shear zone.
Every sheet blade of rotor shearing impeller of the present invention not only has angling hole, also has scarp at its head, alleviate the inertia weight that impeller sheared by rotor, reduce motor power consumption.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is shell knife of the present invention, impeller sheared by stator gear piece and rotor coordinates schematic diagram.
Fig. 3 is the front view that impeller sheared by rotor of the present invention.
Fig. 4 is the front view of shell knife of the present invention.
Fig. 5 is the front view of stator gear piece of the present invention
Wherein: 1, feed cavity; 2, upper cover plectane; 3, chamber is sheared; 4, discharge port; 5, impeller sheared by rotor; 6, motor; 7, drive shaft; 8, shell knife; 9, backgauge annulus; 10, guide annulus; 11, stator gear piece; 12, blade; 13, angling hole; 14, annulus cover; 15, slotted hole; 16, knife edge face; 17, broached-tooth design.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4 and Fig. 5, the spirulina cells broken wall shear of the present embodiment, comprise and shear chamber 3, the top of shearing chamber 3 has been bolted upper cover plectane 2, the chassis in shearing chamber 3 is sealed and installed with rotor by drive shaft 7 and shears impeller 5, drive shaft 7 passes the chassis in shearing chamber 3 and connects motor 6, and drive shaft 7 is tightly connected with the chassis institute perforate of shearing chamber 3; The circumferential direction uniform intervals shearing chassis, chamber 3 is provided with multiple shell knife 8, shell knife 8 head is provided with symmetrical knife edge face 16, blade is formed on top, two knife edge faces 16, be separately installed with stator gear piece 11 between the knife edge face 16 of adjacent two shell knifes 8, the blade of shell knife 8, the blade of stator gear piece 11 and rotor are sheared between impeller 5 and are left gap; The inner face of upper cover plectane 2 is connected with backgauge annulus 9, the bottom of backgauge annulus 9 abuts with shell knife 8, the inner face of upper cover plectane 2 is also fixed with guide annulus 10 by fastening piece, and guide annulus 10 is positioned at rotor and shears the top of impeller 5, and and rotor shear between impeller and leave minim gap; Install feed cavity 1 in the middle part of upper cover plectane 2, the sidewall bottom shearing chamber 3 is provided with discharge port 4.
The structure that impeller 5 sheared by rotor is: comprise annulus cover 14, be tightly connected with drive shaft 7 in the middle part of annulus cover 14, annulus cover 14 outer circumference surface on uniform intervals be provided with multiple blade 12, blade 12 has angling hole 13, angling hole 13 in the middle part of blade 12 to the setting from high to low of head; The head being positioned at blade 12 is provided with inclined-plane.The design of angling hole 13, is conducive to spirulina and throws away along radial direction under rotary centrifugal force effect, with blade mechanics to shock; Under the high speed rotating of rotor shearing impeller 5 drives, obtain radial shear simultaneously; Spirulina clashes in normal direction and obtains effective cytoclasis effect under radial shear dual function.
The structure of shell knife 8 is: shell knife 8 one-tenth rectangular structure, has slotted hole 15 in the middle part of shell knife 8.
The structure of stator gear piece 11 is: the structure in echelon of stator gear piece 11, and the bottom of stator gear piece 11 is formed with broached-tooth design 17.
The knife edge face 16 of shell knife 8 both sides is parallel with the two sides of stator gear piece 11, and the gap between the knife edge face 16 of shell knife 8 both sides and stator gear piece 11 side is 3mm-5mm.
The blade of shell knife 8, the blade of stator gear piece 11 and the rotor gap of shearing between impeller 5 are 0.5mm-1mm.Shell knife 8 and stator gear piece 11 can move adjustment in radial direction, and make the blade of shell knife 8, the blade of stator gear piece 11 is all on same radius of circle.
The knife edge face 16 of shell knife 8 is 15 ° ~ 45 ° with the angle a of blade horizontal direction.The angle formed between such guarantee two knife edge faces 16 is less than the acute angle of 90 °, increases the sharpness of shell knife 8.The slotted hole 15 that shell knife 8 is opened, can ensure when shell knife 8 and the rotor gap of shearing between impeller 5 increases like this, is still fixed on the chassis in shearing chamber 3 with screw after being convenient to mobile shell knife 8.
The knife edge face 16 of shell knife 8 is 30 ° with the angle a of blade horizontal direction.
When stator gear piece 11 and the rotor gap of shearing between impeller 5 increases, backgauge annulus 9 will be pushed against on the chassis in shearing chamber 3.
The installation of backgauge annulus 9 of the present invention effectively prevents the spirulina without shearing broken wall from directly leaving shear zone; Abut with shell knife 8 and stator gear piece 11 bottom backgauge annulus 9, further fixed stator blade 8 and stator gear piece 11 simultaneously.
In actual use procedure, when carrying out cell wall breaking experiment to spirulina, the volume ratio of spirulina and water is 1:0.5, spirulina slurry after being uniformly mixed at the uniform velocity is injected into from feed cavity 1 to be sheared in chamber 3, rotor is sheared impeller 5 and is run up under the driving of motor 6, the centrifugal force that the high-speed rotation that impeller 5 sheared by rotor produces produces negative pressure in shearing chamber 3, guarantees that spirulina slurry is inhaled into smoothly under flow state and shears chamber 3.
Spirulina slurry is under impeller 5 centrifugal action sheared by the rotor run up, the angling hole 13 that major part spirulina slurry shears impeller 5 by rotor is thrown toward blade in jet-like, then slurry shears at rotor the shear zone that impeller 5 forms with shell knife 8, stator gear piece 11, the high-speed rotation of impeller 5 relative stator blade 8, stator gear piece 11 sheared by rotor, and slurry is subject to rotor and shears impeller 5 and cut high speed radial cuts between cutting edge and shell knife 8, stator gear piece 11 place cutting edge; By the comprehensive action of radial shear, normal direction impinging jet, spirulina slurry is made to reach significant superfine grinding effect.
In shear history, sub-fraction slurry is sheared by guide annulus 10 and rotor circular clearance that impeller 5 forms and is entered between the shear zone that rotor shears between impeller 5 and shell knife 8, stator gear piece 11, and this part slurry is subject to high speed radial shear equally.
The present invention shears the gap between impeller 5 and shell knife 8, stator gear piece 11 by periodic adjustment rotor, can ensure a shell-broken effect of spirulina slurry.Spirulina after shearing-crushing is continuously outflowed by the gap between shell knife 8 and stator gear piece 11, discharges after convergence through discharge port 4.
For increasing the effect of spirulina cells broken wall, the spirulina slurry after broken wall can be put into shearing device again and carrying out circulating cells broken wall.Experiment proves, through 2 circulations, spirulina cells particle size after cracking reaches more than 300 orders.Spirulina after fragmentation can enter follow-up Phycocyanins, C-extraction process flow process.
More than describing is explanation of the invention, and be not the restriction to invention, limited range of the present invention, see claim, within protection scope of the present invention, can do any type of amendment.
Claims (7)
1. a spirulina cells broken wall shear, it is characterized in that: comprise and shear chamber (3), the top of described shearing chamber (3) has been bolted upper cover plectane (2), the chassis of described shearing chamber (3) is sealed and installed with rotor by drive shaft (7) and shears impeller (5), described drive shaft (7) passes the chassis of shearing chamber (3) and connects motor (6), and described drive shaft (7) is tightly connected with the chassis institute perforate of shearing chamber (3); The circumferential direction uniform intervals on described shearing chamber (3) chassis is provided with multiple shell knife (8), described shell knife (8) head is provided with symmetrical knife edge face (16), blade is formed on two knife edge face (16) tops, be separately installed with stator gear piece (11) between the knife edge face (16) of adjacent two shell knifes (8), the blade of described shell knife (8), the blade of stator gear piece (11) and rotor are sheared between impeller (5) and are left gap; The inner face of described upper cover plectane (2) is connected with backgauge annulus (9), the bottom of described backgauge annulus (9) abuts with shell knife (8) and stator gear piece (11), the inner face of described upper cover plectane (2) is also fixed with guide annulus (10) by fastening piece, and described guide annulus (10) is positioned at the top that impeller (5) sheared by rotor; Feed cavity (1) is installed at described upper cover plectane (2) middle part, and the sidewall bottom of described shearing chamber (3) is provided with discharge port (4); The structure of described stator gear piece (11) is: the structure in echelon of described stator gear piece (11), and the bottom of described stator gear piece (11) is formed with broached-tooth design (17).
2. spirulina cells broken wall shear as claimed in claim 1, it is characterized in that: the structure that impeller (5) sheared by described rotor is: comprise annulus cover (14), described annulus cover (14) middle part is tightly connected with drive shaft (7), on the outer circumference surface of described annulus cover (14), uniform intervals is provided with multiple blade (12), described blade (12) has angling hole (13), described angling hole (13) is from blade (12) middle part to the setting from high to low of head; The head being positioned at blade (12) is provided with inclined-plane.
3. spirulina cells broken wall shear as claimed in claim 1, is characterized in that: the structure of described shell knife (8) is: described shell knife (8) becomes rectangular structure, described shell knife (8) middle part has slotted hole (15).
4. spirulina cells broken wall shear as claimed in claim 1, it is characterized in that: the knife edge face (16) of described shell knife (8) both sides is parallel with the two sides of stator gear piece (11), the gap between the knife edge face (16) of described shell knife (8) both sides and stator gear piece (11) side is 3mm-5mm.
5. spirulina cells broken wall shear as claimed in claim 1, is characterized in that: the blade of described shell knife (8), the blade of stator gear piece (11) and the rotor gap of shearing between impeller (5) are 0.5mm-1mm.
6. spirulina cells broken wall shear as claimed in claim 1, is characterized in that: the knife edge face (16) of described shell knife (8) and the angle (a) of blade horizontal direction are 15 ° ~ 45 °.
7. spirulina cells broken wall shear as claimed in claim 6, is characterized in that: the knife edge face (16) of described shell knife (8) and the angle (a) of blade horizontal direction are 30 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410511684.1A CN104450509B (en) | 2014-09-29 | 2014-09-29 | Spirulina cells broken wall shear |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410511684.1A CN104450509B (en) | 2014-09-29 | 2014-09-29 | Spirulina cells broken wall shear |
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| CN104450509A CN104450509A (en) | 2015-03-25 |
| CN104450509B true CN104450509B (en) | 2016-02-03 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105054137B (en) * | 2015-08-12 | 2018-08-03 | 徐晟伟 | A kind of seaweed means for breaking walls |
| CN111072170A (en) * | 2019-12-05 | 2020-04-28 | 大连民族大学 | Red tide processing apparatus |
| CN112642537A (en) * | 2020-12-29 | 2021-04-13 | 杨慧慧 | Cell crushing device for cell extraction |
| CN112812176B (en) * | 2021-01-13 | 2022-10-11 | 江南大学 | Method for extracting phycocyanin from spirulina by low-salt flocculation method |
| CN114618655B (en) * | 2021-10-26 | 2023-09-05 | 杭州娃哈哈精密机械有限公司 | Ultrasonic vibration high-speed cutting and crushing device |
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| CN102728273A (en) * | 2012-06-25 | 2012-10-17 | 江南大学 | Granulation equipment for preparing pellets |
| CN104001599A (en) * | 2014-04-30 | 2014-08-27 | 贵州超亚纳米科技有限公司 | Crushing cavity of cyclone crusher and internal cutting tool thereof |
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2014
- 2014-09-29 CN CN201410511684.1A patent/CN104450509B/en active Active
Patent Citations (5)
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|---|---|---|---|---|
| WO1995009051A1 (en) * | 1993-09-28 | 1995-04-06 | Consul T.S. S.R.L. | A mechanical triturator for biological material |
| US6561885B1 (en) * | 1999-05-07 | 2003-05-13 | B. Maier Zerkleinerungstechnik Gmbh | Ring cutter for cutting chips |
| CN201848290U (en) * | 2010-11-03 | 2011-06-01 | 天津市中药机械厂 | Beating plate and cutter head assembly for traditional Chinese medicine crusher |
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Effective date of registration: 20201229 Address after: 53-19-301, xiuxi Road, Wuxi City, Jiangsu Province, 214000 Patentee after: WUXI HOPE LIGHT INDUSTRY EQUIPMENT TECHNOLOGY Co.,Ltd. Address before: No. 1800 road 214122 Jiangsu Lihu Binhu District City of Wuxi Province Patentee before: Jiangnan University |
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