CN104435749B - A kind of dendrobium candidum compound preparation and its preparation method and application - Google Patents
A kind of dendrobium candidum compound preparation and its preparation method and application Download PDFInfo
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- CN104435749B CN104435749B CN201410632144.9A CN201410632144A CN104435749B CN 104435749 B CN104435749 B CN 104435749B CN 201410632144 A CN201410632144 A CN 201410632144A CN 104435749 B CN104435749 B CN 104435749B
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- dendrobium candidum
- american ginseng
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- fruit
- powder
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Abstract
The invention discloses a kind of dendrobium candidum compound preparation and its preparation method and application, said preparation is made up of the raw material of following percentage by weight：Dendrobium candidum 15~30%, the fruit of Chinese wolfberry 10~20%, American Ginseng 10~30%, ganoderma lucidum 10~20% and auxiliary material 5~35%.In the present invention, dendrobium candidum Yin-nourishing and body fluid promoting is nourishing liver and kidney with the fruit of Chinese wolfberry with ganoderma lucidum invigorating qi for tranquilization with American Ginseng boosting qi and nourishing yin, closes and can produce synergy with it, has strengthen immunity, alleviate the healthcare function of physical fatigue.Dendrobium candidum compound preparation of the present invention can be used in the health products and medicine of the gentle physical fatigue of preparation strengthen immunity.The preparation method of dendrobium candidum compound preparation of the present invention, its extracting method is simple, and functional component extraction effect is good, is easy to industrialized production, possesses wide application prospect.
Description
Technical field
The present invention relates to dendrobium candidum compound medicine field, and in particular to a kind of dendrobium candidum compound preparation and its preparation side
Method and application.
Background technology
In recent years, as the development and growth in the living standard of science and technology, the average life span of compatriots constantly extend, many cities
Gradually step into aging, in order to improve the quality of living, strengthen life effect, the health products of reasonable employment illness prevention and fitness oneself gradually into
Requirement is felt for one kind of people, the research and development in this field is also increasingly taken seriously.
Application publication number is CN 102988827A (Application No. CN 201210473859.5) Chinese invention patent Shen
A kind of dendrobium officinale granula please be disclose, is mainly made up of dendrobium candidum, Poria cocos, ginseng, the fruit of Chinese wolfberry, Chinese yam, coix seed etc.,
Described dendrobium candidum, Poria cocos, ginseng, the fruit of Chinese wolfberry, Chinese yam, the weight proportion of coix seed are：15~28：30~42：7~18：3
~13：15~28：15~28.The technical scheme can adjust immunity function, antifatigue, tonifying Qi is supported by the compounding of each medicine
The moon, suitable for system is weak and fatiguability person, particularly to rehabilitation after being ill, overwork, lung-stomach yin deficiency shower have it is good
Effect, spiritlessness and weakness of the people caused by busy working and learning can be eliminated, working and learning efficiency can be effectively improved.
Application publication number is CN 102349975A (Application No. CN 201110306954.1) Chinese invention patent Shen
Please disclose the preparation method and purposes of a kind of Dendrobium officinale health product formula, composition and combination, the formula include monarch drug in a prescription,
Ministerial drug and adjutant, monarch drug in a prescription are dendrobium candidum and cordyceps, and ministerial drug is American Ginseng, and adjutant is the fruit of Chinese wolfberry, and its content is：Fresh iron sheet
50~1000g of the stem of noble dendrobium, 3~9g of cordyceps, 3~6g of American Ginseng, 6~12g of the fruit of Chinese wolfberry.No matter is Dendrobium officinale health product formula
In terms of the traditional Chinese medicine or in terms of pharmacology, at least with strengthen immunity the effect of.
Application publication number is that CN 1457808A (Application No. 02111657.1) Chinese invention patent application discloses one
Kind of dendrobium candidum compound preparation and its preparation and application, said preparation be by percentage by weight be 20%~80% active component with
The oral formulations or drink of 80%~20% pharmaceutic adjuvant composition, wherein, active ingredient is by the iron sheet of percentage by weight 5%~50%
The stem of noble dendrobium, 5~50% stem of noble dendrobium medicinal extract, 5~50% American Ginsengs or American Ginseng medicinal extract composition.Its preparation method includes：(1) by iron sheet
The stem of noble dendrobium picks impurity, the sterilizing of 70% ethanol, waves most ethanol, more than the mesh of ultra-fine grinding 300 standby after drying；(2) stem of noble dendrobium is added with water
Circumfluence distillation, merge extract solution, be dried in vacuo after concentration, it is standby to obtain extract powder；(3) American Ginseng is heated back with 70% ethanol
Stream extraction, merges extract solution, is dried in vacuo after concentration, it is standby to obtain extract powder；(4) dendrobium officinale powder, stone are weighed by recipe quantity
Dry measure used in former times extract powder and American ginseng extract powder, conventional auxiliary material is added, is sufficiently mixed according to a conventional method, particle is made, produce granule；
Particle is inserted in capsulae vacuus, produces capsule；Particle is directly pressed into piece, produces tablet；Slice, thin piece film coating, produce film bag
Garment piece.The dendrobium candidum compound preparation can adjust immunity function and antifatigue, boosting qi and nourishing yin, weak and easily tired suitable for system
Lao Zhe, particularly to rehabilitation after being ill, overwork, lung-stomach yin deficiency shower have the function that it is good.
The content of the invention
It is an object of the invention to provide a kind of dendrobium candidum compound preparation and its preparation method and application, the dendrobium candidum is answered
Square preparation has strengthen immunity, alleviates the healthcare function of physical fatigue.
A kind of dendrobium candidum compound preparation, is made up of the raw material of following percentage by weight：
Preferably, described dendrobium candidum compound preparation, is made up of the raw material of following percentage by weight：
Further preferably, in described dendrobium candidum compound preparation, described dendrobium candidum, the fruit of Chinese wolfberry, American Ginseng and spirit
The mass ratio of sesame is 3：2：3：2.Under the mass ratio, above-mentioned functional component synergy is stronger.
In the present invention, dendrobium candidum Yin-nourishing and body fluid promoting, with American Ginseng boosting qi and nourishing yin, with ganoderma lucidum invigorating qi for tranquilization, grown with the fruit of Chinese wolfberry
Filling liver kidney, close and use it.Four kinds of dendrobium candidum, the fruit of Chinese wolfberry, American Ginseng and ganoderma lucidum can produce synergy into division with it.
Both the body of vital essence deficiency it had been suitable for, and had been suitable for the people of YIN fluid deficiency again, for deficiency of both the lung and kidney, deficiency of both vital energy and Yin, lassitude hypodynamia, waist and knee pain
Bitterly, the few phlegm of cough, dry throat, feeble pulse weak person.Dendrobium candidum compound preparation of the present invention can be used for preparation strengthen immunity to ease up
In the health products and medicine of physical fatigue.
Because active ingredient is not quite similar in raw material contained by compound dendrobium officinale preparation prescription, the contained enhancing of dendrobium candidum is exempted from
Epidemic disease power, it is antifatigue the effect of composition be mainly dendrobium polysaccharide, composition is GL-B the effect of ganoderma lucidum, the composition the effect of fruit of Chinese wolfberry
For Lycium barbarum polysaccharides, thus these three raw materials using water as solvent extraction, and composition removes water-soluble polysaccharide the effect of American Ginseng
Outside, also American ginseng total saponins are liposoluble constituent, therefore first using water extraction again after ethanol water (most preferably 70%) extraction.
A kind of preparation method of dendrobium candidum compound preparation, comprises the following steps：
(1) dendrobium candidum is broken into powder, obtains powdery dendrobium candidum；
(2) American Ginseng is broken into powder, adds the ethanol water heating and refluxing extraction that mass fraction is 60%~80%, system
Extract solution and American Ginseng alcohol extracting slag are obtained, extract solution obtains American Ginseng medicinal extract through being concentrated under reduced pressure；
(3) the American Ginseng alcohol extracting slag in step (2), powdery dendrobium candidum, the fruit of Chinese wolfberry and the ganoderma lucidum in step (1) are added into water
Decoct, obtain decocting liquid, decocting liquid obtains filtrate after filtering, and filtrate obtains the water extracted immersing paste through being concentrated under reduced pressure；
(4) the water extracted immersing paste that the American Ginseng medicinal extract and step (3) obtained step (2) obtains, which merges, to be mixed, dry through spraying
It is dry to obtain pharmaceutical activity powder；
(5) auxiliary material is added in the pharmaceutical activity powder that will be obtained in step (4), prepares dendrobium candidum compound preparation.
In step (1), dendrobium candidum is broken into powder, 30 mesh sieves is crossed, obtains powdery dendrobium candidum.Powdery below the size
Dendrobium candidum is adapted to the extraction of effective ingredient.
In step (2), American Ginseng is broken into powder, addition mass fraction is water-soluble for 60%~80% ethanol after crossing 30 mesh sieves
Liquid heating and refluxing extraction.The American Ginseng powder of appropriate particle size is advantageous to the extraction of American ginseng total saponins liposoluble constituent.
The mass fraction of described ethanol water is 70%, and the ethanol water of mass fraction 70% is very beneficial for the West
Join the extraction of total saposins liposoluble constituent, fully extract active constituents of medicine.
It is 1 in the American Ginseng of powdery and the weight ratio of ethanol water：10~20, be advantageous to extract active constituents of medicine
Out.
Described heating and refluxing extraction is 2~3 times, 2~4 hours every time, merges extract solution.
Temperature of the described extract solution through being concentrated under reduced pressure is 65 DEG C~75 DEG C.
In step (3), described American Ginseng alcohol extracting slag, powdery dendrobium candidum, the fruit of Chinese wolfberry and the gross weight of ganoderma lucidum and water
Weight ratio is 1：25~35, abundant extraction of the weight than being also beneficial to effective ingredient.
Temperature of the described filtrate through being concentrated under reduced pressure is 70 DEG C~80 DEG C.
The described decocting liquid that adds water to cook to obtain is：Add water to cook successively 2~3 times, 3~5 hours every time, merge decoct afterwards
Liquid.
In step (5), described auxiliary material be lactose, sucrose, glucose, starch, dextrin, microcrystalline cellulose, magnesium stearate,
More than one or both of auxiliary materials such as honey, talcum powder, xylitol, mannitol, maltitol, D-sorbite (including two
Kind), processing prepares the preparations such as piece agent, capsule, granule, pill, powder, oral liquid, vina or paste.Wherein, tablet,
The preparation of capsule, pill, granule, powder：Take pharmaceutical activity powder made from step (4) to be well mixed with proper auxiliary materials, press
Photograph answers the processing of formulation common process to be made.The preparation of oral liquid, vina, paste：Pharmaceutical activity powder made from step (4) is taken to press
More solito technique adds water or ethanol to be dissolved, and adds suitable auxiliary material and is well mixed, and processes and makes by corresponding formulation common process
.
In the bulk drug of compound dendrobium officinale preparation of the present invention, the stem of dendrobium candidum system orchid dendrobium candidum, it
It is medicinal first recorded in《Sheng Nong's herbal classic》, and it is listed in top grade.Motherland's medical science thinks stem of noble dendrobium slightly sweet flavor, micro- salty, cold in nature, enters stomach, kidney
Through, it is nontoxic, there is the effect of promoting the production of body fluid beneficial stomach, clearing heat and nourishing yin.Consume and hinder and abnormal heat is not moved back, be irritated thirsty suitable for pyreticosis later stage yin jin
Person.The deficiency of liver-yin and kidney-yin can be controlled, is replenishing the vital essence and removing heat, the key medicine of reinforcing stomach reg fluid.Li Shizhen (1518-1593 A.D.) exists《Compendium of Materia Medica》It is middle to claim it to have " Qiang Yinyi
Essence, thick stomach, mend in never sufficient, flat stomach gas, long muscle, sedate is except shying, macrobiosis of making light of one's life by commiting suicide " siddhi special effect.
Successive dynasties peculiar effect of the medicine and pharmacology works stem of noble dendrobium has clear and definite record, it is believed that it is the superfine product of enriching yin qi-restoratives.It is modern
Medicine drug development proves active component dendrobium polysaccharide contained in the stem of noble dendrobium, has immune dual regulation, to hypoimmunity
Person, the invasion and attack of disease can be resisted by adjusting function of immune system；To immunologic function hyperthyroid, by adjusting immune system work(
Can, recover its normal level.American Ginseng sweet and slightly bitter taste, it is cool in nature, enter cardiopulmonary kidney three and pass through, beneficial lung yin, the clear fire of deficiency type, promote the production of body fluid to quench thirst,《This
Careless detailed outline is picked up any lost article from the road》Calling it, " tonifying lung fall fire, born fluid, relieving restlessness is tired, empty and have fiery person suitable." therefore the people of all fire excess from yin deficiency overstrain coughs,
Often with American Ginseng, then the flat fire of gas is held back, and cough is gradually put down.《Legendary god of farming's book on Chinese herbal medicine》It is " materials for improving vision, tonifying Qi, An Jinghun, strengthening the bones and muscles, good to record ganoderma lucidum
Color, food, makes light of one's life by commiting suicide not old long, macrobiosis angle ".《Compendium of Materia Medica》Referred to as upper medicine, its is warm-natured lightly seasoned, nontoxic, invigorating heart gas,
Bowl spares increases intelligence, and food is made light of one's life by commiting suicide not old long, is promoted longevity.The fruit of Chinese wolfberry is that maturation of the Solanaceae undershrub fruit of Chinese wolfberry is real, can not only be used for heavily fortified point
Fruit eats, and is the traditional Chinese medicine that effect is distinguished simply again, is usually taken as the good medicine of nourishing maintenance and anti-aging.Pharmacology at all times
Proved with clinical research, the fruit of Chinese wolfberry truly has the effect of preventing and curing diseases, promoting longevity.Dendrobium candidum enriching yin is given birth in this compound preparation
Tianjin, it is nourishing liver and kidney with the fruit of Chinese wolfberry with ganoderma lucidum invigorating qi for tranquilization with American Ginseng boosting qi and nourishing yin, close and use it.Both it had been suitable for vital essence deficiency
Body, it is suitable for the people of YIN fluid deficiency again, for deficiency of both the lung and kidney, deficiency of both vital energy and Yin, lassitude hypodynamia, lumbocrural pain, few phlegm of coughing, dry throat mouth
It is dry, feeble pulse weak person.
Compared with prior art, have the following advantages that：
In the present invention, dendrobium candidum Yin-nourishing and body fluid promoting, with American Ginseng boosting qi and nourishing yin, with ganoderma lucidum invigorating qi for tranquilization, grown with the fruit of Chinese wolfberry
Filling liver kidney, close and use it.Four kinds of dendrobium candidum, the fruit of Chinese wolfberry, American Ginseng and ganoderma lucidum can produce synergy into division with it.
Both the body of vital essence deficiency it had been suitable for, and had been suitable for the people of YIN fluid deficiency again, for deficiency of both the lung and kidney, deficiency of both vital energy and Yin, lassitude hypodynamia, waist and knee pain
Bitterly, the few phlegm of cough, dry throat, feeble pulse weak person.Dendrobium candidum compound preparation of the present invention is applied to immunocompromised person, fatiguability
Person, the elderly and the various people in sub-health state, it may also be used for the rehabilitation after cancer patient chemicotherapy.
The preparation method of dendrobium candidum compound preparation of the present invention, it prepares simply, is easy to industrialized production, possesses wide
Application prospect.
Brief description of the drawings
Fig. 1 is the process chart of the preparation method of dendrobium candidum compound preparation of the present invention.
Embodiment
The invention will be further described with reference to embodiments, but these embodiments must not be used to explain to present invention guarantor
Protect the limitation of scope.
Embodiment 1
As shown in figure 1, prepare dendrobium candidum compound granule according to the production technology shown in Fig. 1；
The present embodiment dendrobium candidum compound granule, its bulk drug proportioning are：
Main ingredient：Dendrobium candidum 30g, American Ginseng 30g, the fruit of Chinese wolfberry (fruit of Chinese wolfberry medicine materical crude slice) 20g, ganoderma lucidum (lucid ganoderma decoction piece) 10g；It is auxiliary
Material：DEXTRIN g.
Prepared according to following concrete modes：
(1) dendrobium candidum, ginseng powder are broken into coarse powder, cross 30 mesh sieves, standby, obtain powdery dendrobium candidum and American Ginseng is thick
Powder；
(2) American Ginseng coarse powder, aqueous solution heating and refluxing extraction 2 time of 16 times of amount mass percents for 70% ethanol is added, i.e.,
It is 1 in the American Ginseng of powdery and the weight ratio of ethanol water：16,3 hours every time, extract solution and American Ginseng alcohol extracting slag is made,
Merge extract solution, 65 DEG C~75 DEG C decompression (- 0.04~-0.08MPa) concentrations, reclaim ethanol, obtain American Ginseng medicinal extract (medicinal extract I),
It is standby；
(3) American Ginseng alcohol extracting slag is together with powdery dendrobium candidum, fruit of Chinese wolfberry medicine materical crude slice, lucid ganoderma decoction piece, adds the pure of 30 times of weight
Change decocting to boil 2 times, i.e. the weight ratio of American Ginseng alcohol extracting slag, powdery dendrobium candidum, the fruit of Chinese wolfberry and the gross weight of ganoderma lucidum and water is 1：
30,4 hours every time, collecting decoction, filter, 70 DEG C~80 DEG C of filtrate is concentrated under reduced pressure (- 0.04~-0.08MPa), obtains the water extracted immersing paste
(medicinal extract II), it is standby；
(4) medicinal extract obtained by step (2) and (3) is merged and mixed, it is spray-dried to obtain extract fine powder (i.e. extract powder), mistake
80 mesh sieves, it is standby；
(5) by extract fine powder obtained by step (4) and excessively, the dextrin of 80 mesh sieves is whole in boiling granulating on fluid bed granulator
Grain, obtains dendrobium candidum compound granule.
The present embodiment dendrobium candidum compound granule, adult are taken twice daily, each 3g.
Embodiment 2
The present embodiment dendrobium candidum compound granule, its bulk drug proportioning are：
Main ingredient：Dendrobium candidum 30g, American Ginseng 20g, the fruit of Chinese wolfberry (fruit of Chinese wolfberry medicine materical crude slice) 20g, ganoderma lucidum (lucid ganoderma decoction piece) 20g；It is auxiliary
Material：Glucose 40g.
Preparation method is the same as embodiment 1.
Embodiment 3
The present embodiment dendrobium candidum composite tablet, its bulk drug proportioning are：
Main ingredient：Dendrobium candidum 30g, the fruit of Chinese wolfberry (fruit of Chinese wolfberry medicine materical crude slice) 20g, American Ginseng 30g, ganoderma lucidum (lucid ganoderma decoction piece) 20g；It is auxiliary
Material：D-mannital 20g, magnesium stearate 0.5g.
Preparation method：
(1) dendrobium candidum, ginseng powder are broken into coarse powder, cross 30 mesh sieves, standby, obtain powdery dendrobium candidum and American Ginseng is thick
Powder；
(2) American Ginseng coarse powder, adds 16 times of 70% ethanol water heating and refluxing extractions of amount (weight) mass fraction 2 times, often
Secondary 3 hours, extract solution and American Ginseng alcohol extracting slag is made, merges extract solution, 65 DEG C~75 DEG C are concentrated under reduced pressure, and reclaim ethanol, obtain western
American ginseng medicinal extract, it is standby；
(3) American Ginseng alcohol extracting slag adds 30 times of amount decoctings to boil 2 together with powdery dendrobium candidum, fruit of Chinese wolfberry medicine materical crude slice, lucid ganoderma decoction piece
It is secondary, 4 hours every time, collecting decoction, filter, 70 DEG C~80 DEG C of filtrate is concentrated under reduced pressure, and obtains American Ginseng medicinal extract, standby；
(4) medicinal extract obtained by step (2) and (3) is merged and mixed, it is spray-dried to obtain extract fine powder, 80 mesh sieves are crossed, it is standby
With；
(5) by extract fine powder obtained by step (4) with crossing the D-mannital of 80 mesh sieves in being seethed with excitement on fluid bed granulator
Granulation, whole grain, obtains particle, standby；
(6) particle obtained by step (5) is well mixed with crossing the magnesium stearate of 80 mesh sieves, tabletting, packaging, produces compound
Dendrobium candidum tablet.
The present embodiment, adult are taken twice daily, and 3 tablets once.
Embodiment 4
The present embodiment dendrobium candidum compound capsule, its bulk drug proportioning are：
Main ingredient：Dendrobium candidum 30g, American Ginseng 30g, the fruit of Chinese wolfberry (fruit of Chinese wolfberry medicine materical crude slice) 20g, ganoderma lucidum (lucid ganoderma decoction piece) 20g；It is auxiliary
Material：Microcrystalline cellulose 10g, superfine silica gel powder 0.5g.
Preparation method：
(1) dendrobium candidum, ginseng powder are broken into coarse powder, cross 30 mesh sieves, standby, obtain powdery dendrobium candidum and American Ginseng is thick
Powder；
(2) American Ginseng coarse powder, adds 16 times of 70% ethanol water heating and refluxing extractions of amount (weight) mass percent 2 times,
3 hours every time, extract solution and American Ginseng alcohol extracting slag is made, merges extract solution, 65 DEG C~75 DEG C are concentrated under reduced pressure, and reclaim ethanol, obtain
American Ginseng medicinal extract, it is standby；
(3) American Ginseng alcohol extracting slag adds 30 times of amount decoctings to boil 2 together with powdery dendrobium candidum, fruit of Chinese wolfberry medicine materical crude slice, lucid ganoderma decoction piece
It is secondary, 4 hours every time, collecting decoction, filter, 70 DEG C~80 DEG C of filtrate is concentrated under reduced pressure, and obtains the water extracted immersing paste, standby；
(4) medicinal extract obtained by step (2) and (3) is merged and mixed, it is spray-dried to obtain extract fine powder, 80 mesh sieves are crossed, it is standby
With；
(5) by extract fine powder obtained by step (4) with crossing the microcrystalline cellulose of 80 mesh sieves in being seethed with excitement on fluid bed granulator
Granulation, obtains particle, standby；
(6) particle obtained by step (5) is well mixed with crossing the superfine silica gel powder of 80 mesh sieves, filling capsule, packaging, produced
Compound dendrobium officinale capsule.
The present embodiment, adult are taken twice daily, 3 tablets each time.
Comparative example 1
The present embodiment dendrobium candidum compound granule, its bulk drug proportioning are：
Main ingredient：Dendrobium candidum 34g, American Ginseng 34g, the fruit of Chinese wolfberry (fruit of Chinese wolfberry medicine materical crude slice) 22g；Auxiliary material：DEXTRIN g.
Prepared according to following concrete modes：
(1) dendrobium candidum, ginseng powder are broken into coarse powder, cross 30 mesh sieves, standby, obtain powdery dendrobium candidum and American Ginseng is thick
Powder；
(2) American Ginseng coarse powder, aqueous solution heating and refluxing extraction 2 time of 16 times of amount mass percents for 70% ethanol is added, i.e.,
It is 1 in the American Ginseng of powdery and the weight ratio of ethanol water：16,3 hours every time, extract solution and American Ginseng alcohol extracting slag is made,
Merge extract solution, 65 DEG C~75 DEG C are concentrated under reduced pressure, and reclaim ethanol, obtain American Ginseng medicinal extract, standby；
(3) American Ginseng alcohol extracting slag adds the purified water of 30 times of weight to decoct 2 together with powdery dendrobium candidum, fruit of Chinese wolfberry medicine materical crude slice
Secondary, i.e., the weight ratio of American Ginseng alcohol extracting slag, the gross weight of powdery dendrobium candidum and the fruit of Chinese wolfberry and water is 1：30,4 hours every time, close
It is standby and decocting liquid, filtration, 70 DEG C~80 DEG C of filtrate are concentrated under reduced pressure, and obtain the water extracted immersing paste；
(4) medicinal extract obtained by step (2) and (3) is merged and mixed, it is spray-dried to obtain extract fine powder, 80 mesh sieves are crossed, it is standby
With；
(5) by extract fine powder obtained by step (4) and excessively, the dextrin of 80 mesh sieves is whole in boiling granulating on fluid bed granulator
Grain, obtains dendrobium candidum plain particles agent.
The test of pesticide effectiveness
Animal experiment proves that this product has strengthen immunity and function of relieving physical fatigue.
First, strengthen immunity function test
This product of orally administration mouse various dose 1 month, as a result shows, this product has rise hydrocortisone mould
Type mouse peripheral blood leukocyte count, increase model mouse thymus gland, index and spleen index, strengthen the phagocytic function of macrophage, promote model
Mouse cellular immune function.It is taken as that this product has the function of strengthen immunity.
1 material and method
1.1 material
1.1.1 sample：Dendrobium candidum compound granule prepared by embodiment 1, specification：4.0g/ bags, in June, 2013 trial-production；
Dendrobium candidum plain particles agent prepared by comparative example 1.
1.1.2 experimental animal：Cleaning grade ICR mouse 180, male and female half and half, 18~22g of body weight.By medical science section of Zhejiang Province
Institute's Experimental Animal Center provides, animal quality certification number：SCXK (Zhejiang) 2008-0033.
1.1.3 main agents：India ink, sheep red blood cell (SRBC) (SRBC).
1.1.4 key instrument：Assay balance, spectrophotometer, micro syringe (50ul), slide measure (precision
0.02mm), electron microscope.
1.2 method
1.2.1 healthy mice 60 is taken to hydrocortisone model mice leukocyte count and thymus gland, the influence of index and spleen index,
Male and female half and half, 6 groups are randomly divided into by body weight, is shown in Table 1.Normal group, the daily gavage of model control group mouse give physiological saline
0.01mL·g-1, by the dosage of table 1, gavage gives dendrobium candidum compound granular to administration group respectively, i.e. dosage is respectively 0.4g
kg-1Body weight, 1.2gkg-1Body weight and 4.0gkg-1Body weight, respectively low dosage administration group, middle dosage administration group and high dose
Administration group, daily 1 time, continuous 30 days.Dendrobium candidum plain particles agent prepared by comparative example 1 is used with 1.2gkg-1Body weight is fed
Medicine, it is 1 group of comparative example.In addition to normal group, other each groups start that hydrocortisone (40mg is subcutaneously injected on the 18th day in experiment
kg-1) 5 times, inject 1 time every other day.After last dose 1.5h, mouse orbit blood sampling, leukocyte count is determined, and with cervical dislocation
Dead animal, take thymus gland, spleen and weigh, calculate organ index, then examined with t and carry out statistical disposition.
1.2.2 it is same to the influence mice group of hydrocortisone model mice macrophage phagocytic function, administration and modeling
Test 1.2.1.After last dose 1h, each group mouse tail vein injection india ink (using 4 times of normal saline dilution) 0.01mL
g-1, eye socket takes blood 20uL respectively when 2min, 10min after injection, is dissolved in 2mL 0.1% (wt) aqueous sodium carbonate, shakes up,
The colorimetric at 600nm wavelength, measure absorb angle value, calculate phagocytic index a.Phagocytic index a=(logA1-logA2) ÷ (t1-
t2)。
1.2.3 the influence to sheep red blood cell (SRBC) (SRBC) inducing mouse swelling degree of the paw takes healthy mice 60, and male and female are each
Half, 6 groups are randomly divided into by body weight, is shown in Table 3.Normal group, the daily gavage physiological saline 0.01mLg of model group mouse-1, administration group
Dendrobium candidum compound granular 0.4gkg is pressed respectively-1Body weight, 1.2gkg-1Body weight and 4.0gkg-1The dosage gavage of body weight is given
Medicine, 1 group of comparative example is according to dendrobium candidum plain particles agent 1.2gkg-1The dosage gastric infusion of body weight.1 time a day, continuous 30
My god.After 30 days, in addition to normal group, 2% (wt) SRBC is injected intraperitoneally, every mouse injects 0.2ml (about 1 × 108Individual SRBC).Exempt from
After epidemic disease 4 days, left back foot plantar thickness or swelling are measured, 20% (V/V) SRBC, every mouse then is subcutaneously injected in measuring point
20ul (about 1 × 108Individual SRBC), 24h determines left back foot plantar thickness or swelling, same Site Determination and three times, taken after injection
Average value.
2 results
2.1 pairs of murine interleukin numbers and thymus gland, the influence of index and spleen index
As a result as shown in table 1, compared with model control group, dendrobium candidum compound granular 1.2gkg-1、4.0g·kg-1Energy
The drastically decline of the peripheral white blood cell caused by hydrocortisone is effectively resisted, mitigates the secondary work of hydrocortisone application
With, and can effectively suppress the reduction of immune organ secretion lymphocyte caused by hydrocortisone；Compared with Normal group, iron
Skin stem of noble dendrobium compound granular 1.2gkg-1、4.0g·kg-1The effect of peripheral white blood cells can be relatively significantly lifted, is promoted immune
Propagation (the P of organ<0.05).For mean+SD.
The dendrobium candidum compound granular of table 1 to model mice leukocyte count and thymus gland, index and spleen index influence (n=10,)
Note：Compared with normal group, ##：P<0.01；Compared with model group, *：P<0.05.
The influence of 2.2 pairs of hydrocortisone model mice carbonic clearance indexes
As a result as shown in table 2, compared with model control group, dendrobium candidum compound granular 1.2gkg-1、4.0g·kg-1Energy
Effectively suppress caused mouse carbon particle clearance speed after hydrocortisone adds to slow down；Compared with Normal group, dendrobium candidum
Compound granular 1.2gkg-1、4.0g·kg-1Grazing rate a can be significantly improved, increases mouse carbon particle clearance ability.
Influence of the dendrobium candidum compound granular of table 2 to hydrocortisone model mice carbon particle clearance speed
(n=10,)
| Group | Dosage/gkg-1 | Phagocytic index a |
| Normal group | - | 4.478±0.44 |
| Model control group | - | 3.528±0.93## |
| Low dosage administration group | 0.4 | 3.824±0.71 |
| Middle dosage administration group | 1.2 | 4.314±0.78 |
| High dose administration group | 4.0 | 4.361±0.61* |
| 1 group of comparative example | 1.2 | 3.801±0.67 |
Note：Compared with normal group, ##：P<0.01；Compared with model group, *：P<0.05.
The influence of 2.3 pairs of sheep red blood cell (SRBC) (SRBC) inducing mouse swelling degree of the paw
As a result as shown in table 3, the swelling degree of the paw of model control group has significant significant difference (P compared with control group
<0.01).Dendrobium candidum compound granular 1.2gkg-1、4.0g·kg-1Sheep red blood cell (SRBC) (SRBC) sensitization can be substantially reduced to cause
Pedal swelling degree, effectively facilitate cellular immunity.Compared with normal group, dendrobium candidum compound granular 1.2gkg-1、
4.0g·kg-1Mouse delayed allergy can be effectively facilitated, promotes cellular immune function (P<0.05, P<0.01).
Influence of the dendrobium candidum compound granular of table 3 to sheep red blood cell (SRBC) inducing mouse swelling degree of the paw
(n=10,)
| Group | Dosage/gkg-1 | Swelling/% |
| Normal group | - | 18.75±3.88 |
| Model control group | - | 30.90±4.56## |
| Low dosage administration group | 0.4 | 26.72±7.92 |
| Middle dosage administration group | 1.2 | 24.37±7.83* |
| High dose administration group | 4.0 | 20.42±5.12** |
| 1 group of comparative example | 1.2 | 26.12±7.77* |
Note：Compared with normal group, ##：P<0.01；Compared with model group, *：P<0.05, * *：P<0.01.
3rd, conclusion
This research prompting dendrobium candidum compound granule has rise hydrocortisone model mice peripheral white blood cell,
Increase model mouse thymus gland, index and spleen index, strengthen the phagocytic function of macrophage, promote model mouse cellular immune function.Show this
Preparation has immunological enhancement, can strengthen the specificity and non-specific immune function of body.And its effect is obvious excellent
In 1 group of comparative example, it is seen then that in the present invention, dendrobium candidum Yin-nourishing and body fluid promoting, with American Ginseng boosting qi and nourishing yin, with ganoderma lucidum invigorating qi for tranquilization, match somebody with somebody
The fruit of Chinese wolfberry is nourishing liver and kidney, and four kinds can produce synergy into division with it, have good immunological enhancement, preferably
Strengthen the specificity and non-specific immune function of body.
Claims (1)
- A kind of 1. dendrobium candidum compound preparation answering in the health products and medicine for preparing the gentle physical fatigue of strengthen immunity With, it is characterised in that the bulk drug of the dendrobium candidum compound preparation, which matches, is：Main ingredient：Dendrobium candidum 30g, American Ginseng 30g, the fruit of Chinese wolfberry medicine materical crude slice 20g, lucid ganoderma decoction piece 10g；Auxiliary material：DEXTRIN g；Described dendrobium candidum compound preparation is prepared according to following concrete modes：(1) dendrobium candidum, ginseng powder are broken into coarse powder, cross 30 mesh sieves, standby, obtain powdery dendrobium candidum and American Ginseng coarse powder；(2) American Ginseng coarse powder, aqueous solution heating and refluxing extraction 2 time of 16 times of amount mass percents for 70% ethanol is added, i.e., in powder The American Ginseng of shape and the weight ratio of ethanol water are 1：16,3 hours every time, extract solution and American Ginseng alcohol extracting slag is made, merges Extract solution, 65 DEG C~75 DEG C are decompressed under -0.04~-0.08MPa and concentrate, and reclaim ethanol, it is standby to obtain American Ginseng medicinal extract I；(3) American Ginseng alcohol extracting slag adds the purified water of 30 times of weight together with powdery dendrobium candidum, fruit of Chinese wolfberry medicine materical crude slice, lucid ganoderma decoction piece Decoct 2 times, i.e. the weight ratio of American Ginseng alcohol extracting slag, powdery dendrobium candidum, the fruit of Chinese wolfberry and the gross weight of ganoderma lucidum and water is 1：30, often Secondary 4 hours, collecting decoction, filtration, 70 DEG C~80 DEG C of filtrate, which is decompressed under -0.04~-0.08MPa, to be concentrated, and obtains the water extracted immersing paste II It is standby；(4) medicinal extract obtained by step (2) and (3) is merged and mixed, it is spray-dried to obtain extract fine powder, 80 mesh sieves are crossed, it is standby；(5) by extract fine powder obtained by step (4) with crossing the dextrin of 80 mesh sieves in boiling granulating on fluid bed granulator, whole grain, Obtain dendrobium candidum compound granule.
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| CN109363174A (en) * | 2018-10-31 | 2019-02-22 | 吉林省恒实传食品科技发展有限公司 | A kind of strengthen immunity, the health care's food for alleviating physical fatigue and preparation method thereof |
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