CN104429592A - Ganoderma lucidum culture medium and ganoderma lucidum cultivation method - Google Patents
Ganoderma lucidum culture medium and ganoderma lucidum cultivation method Download PDFInfo
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- CN104429592A CN104429592A CN201410626258.2A CN201410626258A CN104429592A CN 104429592 A CN104429592 A CN 104429592A CN 201410626258 A CN201410626258 A CN 201410626258A CN 104429592 A CN104429592 A CN 104429592A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention belongs to the technical field of traditional Chinese medicine, and particularly provides a ganoderma lucidum culture medium and a ganoderma lucidum cultivation method. The method comprises the steps of obtaining the ganoderma lucidum culture medium which comprises, by weight, 10-80 parts of mulberry twigs, 8-50 parts of rhizoma smilacis glabrae, 8-50 parts of silkworm excrement, 5-30 parts of prunella vulgaris and 5-40 parts of dayflower; smashing and mixing mulberry twigs, rhizoma smilacis glabrae, prunella vulgaris and dayflower, then adding silkworm excrement, stirring the mixture, adding distilled water, stirring the mixture again, measuring the water content of the culture medium, and controlling the water content between 50% and 60%; placing the culture medium in a plastic packaging bag, pumping out the air in the bag, filling the bag with ammonia gas, and sealing the opening of the bag so that a culture medium storage bag can be obtained; placing the storage bag in a room for five to seven days, taking out the original ganoderma lucidum culture medium, conducting sterilization with ultraviolet light for 12-48 h to obtain a sterilized ganoderma lucidum culture medium, placing the sterilized ganoderma lucidum culture medium in a cultivation bag, and then putting ganoderma lucidum strain in the ganoderma lucidum cultivation bag for cultivation. The components of the ganoderma lucidum culture medium are novel and simple, germ breeding of the ganoderma lucidum culture medium can be restrained, the probability that the ganoderma lucidum strain is infected with parasites or other germs can be reduced greatly, and then the yield of finished ganoderma lucidum products can be increased.
Description
Technical field
The invention belongs to technical field of medicine cultivation, be specifically related to medium and the cultivation method of a kind of glossy ganoderma.
Background technology
Glossy ganoderma is Basidiomycetes Polyporaceae Ganoderma fungi, modern medicine study shows, the pharmaceutical component of glossy ganoderma is very abundant, wherein effective ingredient comprises GL-B, glossy ganoderma polypeptide, triterpenes, amino acid, protein, steroid class, mannitol, coumarin glycosides, alkaloid, organic acid (main containing fumaric acid), and various trace elements etc.Glossy ganoderma has dual regulation to human body, kind of curing the disease, and relates to each systems such as cardiovascular and cerebrovascular, digestion, nerve, internal secretion, breathing, motion, especially very remarkable to the preventive and therapeutic effect of tumour, hepatic disease, insomnia and aging.To this, glossy ganoderma is widely used in the treatment of clinical disease.
At present, commercial type be divided into Wild ganoderma and artificial culture glossy ganoderma.On the base portion that Wild ganoderma is born in broad-leaved tree or rotten wood, on the rotten scar of trees, be sometimes also born on coniferous tree.Along with the medical value of glossy ganoderma is extensively familiar with by people, the Wild ganoderma resource of remote, thickly forested mountains is also in continuous minimizing now, although annual glossy ganoderma fertility is not weak, how also seed ejection, also prematurity just not to be plucked by people, so Wild ganoderma resource is falling sharply now year by year.For ensureing the normal medicine resource supply of glossy ganoderma, people are to the laggard row artificial cultivation of the wild training of glossy ganoderma.At present, glossy ganoderma artificial cultivation has segment wood cultivated and cultivating in bag, the segment wood cultivated method referring to the employing preferential species of tree section of being cut into cultivating ganoderma.Common are long section wood cultivation in raw material, short section of wooden cultivation in raw material, short section of wooden grog cultivation, stub cultivation and the cultivation of branch bundle etc. in segment wood cultivated.Although cultivating in bag ratio life cultivation complex procedures, power consumption is large, demands strict technology, and cultivating in bag has a bacterium speed soon, and mycelia distribution area in Duan Mu is wide, and nutrient accumulation is many, with short production cycle, produces the advantages such as comparatively stable and easy acquisition good quality and high output.But there is parasite or other germ due to the matrix of glossy ganoderma in existing above-mentioned two kinds of glossy ganoderma artificial cultivation methods, the lucidum strain after transplanting is caused to infect easily different classes of disease, its living contaminants is all higher, Ganoderma Lucidum mycelia is caused to be disintegrated, Nutrient medium wrecks, time serious, matrix is gone mouldy smelly, and then affects the production of its glossy ganoderma further.Therefore, need in industrialized production a kind of newly can suppress glossy ganoderma matrix germ reproduction, reduce Medium for Ganoderma lucidum and cultivation method thereof that lucidum strain infects parasite or other germ.
Summary of the invention
For the matrix solving prior art glossy ganoderma exists parasite or other germ, the lucidum strain after transplanting is caused easily to infect the technical problem of different classes of disease.The invention provides one and can suppress glossy ganoderma matrix germ reproduction, reduce Medium for Ganoderma lucidum and cultivation method thereof that lucidum strain infects parasite or other germ.
The object of the invention is to be achieved through the following technical solutions: the cultivation method of a kind of glossy ganoderma, it comprises the following steps:
S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 50-300 weight portion, ramulus mori 10-80 weight portion, smilax 8-50 weight portion, silkworm excrement 8-50 weight portion, selfheal 5-30 weight portion, dayflower 5-30 weight portion by weight, for subsequent use;
S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 50-65%, then piles vexed 6-15h;
S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 24-72h;
S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket;
The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, relative moisture control 75-90% is dark;
S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
Preferably, the composts or fertilisers of cultivating in S1 step and weight proportion thereof are: cotton seed hulls 80-200 weight portion, ramulus mori 10-80 weight portion, smilax 8-50 weight portion, silkworm excrement 8-50 weight portion, selfheal 5-30 weight portion, dayflower 5-30 weight portion.
Preferably, the composts or fertilisers of cultivating in S1 step and weight proportion thereof are: cotton seed hulls 100-150 weight portion, ramulus mori 30-60 weight portion, smilax 12-40 weight portion, silkworm excrement 12-40 weight portion, selfheal 10-20 weight portion, dayflower 10-20 weight portion.
Preferably, the composts or fertilisers of cultivating in S1 step and weight proportion thereof are: cotton seed hulls 120 weight portion, ramulus mori 50 weight portion, smilax 20 weight portion, silkworm excrement 20 weight portion, selfheal 15 weight portion, dayflower 15 weight portion.
Preferably, the water content in S2 step composts or fertilisers of cultivating, at 55-60%, then piles vexed 8-12h.
Preferably, after S3 step piles vexed end, composts or fertilisers of cultivating is loaded in choke protective bag, pumps in choke protective bag after air, in bag, be filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 36-48h.
Preferably, S4 step postvaccinal kind of pocket traverse is in fungus shelf, and indoor temperature controls between 28-32 DEG C, relative moisture control 80-85%.
Ramulus mori of the present invention is the spray of moraceae plants mulberry, and micro-hardship, property are flat, return liver, lung channel, have the effect of wines used as antirheumatic, sharp joint, row aqueous vapor; Smilax is the dry rhizome of dicotyledon medicine liliaceous plant smilacis glabra, sweet, light, flat, returns liver, stomach, the spleen channel, has the effect in removing toxic substances dehumidifying, sharp joint; Silkworm excrement is the dry ight soil of Bombycidae insect bombyx mori larva, pungent, sweet, warm, returns spleen, stomach warp, have dispel rheumatism, regulating the function of the stomach to resolve the turbidity, effect promoting blood circulation and removing obstruction in channels; Selfheal is the herb of Labiatae Prunella, and bitter, acrid, cold return liver, gallbladder channel, has and lets out clearly irascibility, mass dissipating and swelling eliminating, clearing heat and detoxicating effect; Dayflower is the dry aerial parts of Commelianaceae plant dayflower, sweet, the micro-hardship of taste, cold in nature, has swelling diuretic, clearing heat and detoxicating effect.Sterilization treatment of the present invention can be any one method in normal-pressure sterilization, autoclaving or low pressure sterilizing.Whether thorough sterilizing is also is the key of cultivating ganoderma success or failure, and want timely sterilizing after installing kind of pocket, want to leave space between bag and bag during sterilizing code bag, autoclaving will put clean cold air, falsely presses sterilizing not thorough in order to avoid cause.Normal-pressure sterilization can use low-pressure polyethylene, polyacrylic sack, and autoclaving needs to use high-pressure polypropylene sack, can pack by hand and machine also can be used to pack to raise the efficiency.Except above-mentioned sterilizing methods, also can first kind of pocket and composts or fertilisers of cultivating be packed after sterilizing separately separately, the temperature for composts or fertilisers of cultivating pack is advisable when being cooled to below 30 DEG C again.
The invention has the beneficial effects as follows: Ganodermataceae Dook culture material novel formula of the present invention, composition is simple, can suppress the germ reproduction of glossy ganoderma matrix, effectively can reduce the probability that lucidum strain infects parasite or other germ, can improve glossy ganoderma production.The cultivation method of glossy ganoderma of the present invention simply, cultivate and cultivation, and it is fast that the glossy ganoderma that the inventive method is cultivated has a bacterium speed by the glossy ganoderma being applicable to the different cultivars such as red sesame, Ganoderma Sinense, Ganoderma capuse, insect pest disease is few, nutrient accumulation is many, with short production cycle, produces more stable advantage.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated:
embodiment 1:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion are: cotton seed hulls 50g, ramulus mori 10g, smilax 8g, silkworm excrement 8g, selfheal 5g, dayflower 5g.
embodiment 2:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion are: cotton seed hulls 300g, ramulus mori 80g, smilax 50g, silkworm excrement 50g, selfheal 30g, dayflower 30g.
embodiment 3:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion are: cotton seed hulls 80g, ramulus mori 30g, smilax 12g, silkworm excrement 12g, selfheal 10g, dayflower 10g.
Embodiment 4:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion are: cotton seed hulls 100g, ramulus mori 60g, smilax 40g, silkworm excrement 40g, selfheal 20g, dayflower 20g.
embodiment 5:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion are: cotton seed hulls 120g, ramulus mori 50g, smilax 20g, silkworm excrement 20g, selfheal 15g, dayflower 15g.
embodiment 6:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 50g, ramulus mori 10g, smilax 8g, silkworm excrement 8g, selfheal 5g, dayflower 5g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 50-65%, then piles vexed 6-15h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 24-72h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, relative moisture control 75-90% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
embodiment 7:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 300g, ramulus mori 80g, smilax 50g, silkworm excrement 50g, selfheal 30g, dayflower 30g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 50-65%, then piles vexed 6-15h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 24-72h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, relative moisture control 75-90% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
embodiment 8:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 80g, ramulus mori 30g, smilax 12g, silkworm excrement 12g, selfheal 10g, dayflower 10g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 50-65%, then piles vexed 6-15h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 24-72h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, relative moisture control 75-90% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
Embodiment 9:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 100g, ramulus mori 60g, smilax 40g, silkworm excrement 40g, selfheal 20g, dayflower 20g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 50-65%, then piles vexed 6-15h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 24-72h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, relative moisture control 75-90% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
embodiment 10:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 120g, ramulus mori 50g, smilax 20g, silkworm excrement 20g, selfheal 15g, dayflower 15g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 50-65%, then piles vexed 6-15h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 24-72h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, relative moisture control 75-90% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
embodiment 11:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 50g, ramulus mori 10g, smilax 8g, silkworm excrement 8g, selfheal 5g, dayflower 5g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 55-60%, then piles vexed 8-12h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 36-48h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 28-32 DEG C, relative moisture control 80-85% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
embodiment 12:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 300g, ramulus mori 80g, smilax 50g, silkworm excrement 50g, selfheal 30g, dayflower 30g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 55-60%, then piles vexed 8-12h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 36-48h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 28-32 DEG C, relative moisture control 80-85% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
embodiment 13:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 80g, ramulus mori 30g, smilax 12g, silkworm excrement 12g, selfheal 10g, dayflower 10g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 55-60%, then piles vexed 8-12h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 36-48h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 28-32 DEG C, relative moisture control 80-85% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
Embodiment 14:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 100g, ramulus mori 60g, smilax 40g, silkworm excrement 40g, selfheal 20g, dayflower 20g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 55-60%, then piles vexed 8-12h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 36-48h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 28-32 DEG C, relative moisture control 80-85% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
embodiment 15:
A cultivation method for glossy ganoderma, it comprises the following steps: S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 120g, ramulus mori 50g, smilax 20g, silkworm excrement 20g, selfheal 15g, dayflower 15g by weight, for subsequent use; S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 55-60%, then piles vexed 8-12h; S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 36-48h; S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket; The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 28-32 DEG C, relative moisture control 80-85% is dark; S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
Effect of the present invention is further illustrated below by experiment:
The impact that experiment one, composts or fertilisers of cultivating of the present invention and cultivation method grow Ganoderma Lucidum
1 materials and methods
Culture material formula: experiment A group: cotton seed hulls 20kg, ramulus mori 8kg, smilax 5kg, silkworm excrement 5kg, selfheal 3kg, dayflower 3kg; Experiment B group: cotton seed hulls 12kg, ramulus mori 5kg, smilax 2kg, silkworm excrement 2kg, selfheal 1.5kg, dayflower 1.5kg; Experiment C group: cotton seed hulls 10kg, ramulus mori 3kg, smilax 1.2kg, silkworm excrement 1.2kg, selfheal 1kg, dayflower 1kg; Experiment D group: cotton seed hulls medium: cotton seed hulls 10kg, wheat bran 1kg, glucose 2kg, agar 2kg; Experiment E group: maize powder medium: corn flour 5kg, peptone 0.5kg, MgSO40.05kg, KH2PO40.15kg, glucose 2kg, agar 2kg.The concrete preparation method of experiment A, B, C group, pulverize after cotton seed hulls, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, measure the water content in composts or fertilisers of cultivating after adding distilled water stirring, water content control, at 50-65%, then piles vexed 6-15h; After piling vexed end, composts or fertilisers of cultivating is loaded in choke protective bag, pumps in choke protective bag after air, in bag, be filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 24-72h; Composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out conventional sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket.The cotton seed hulls medium of experiment D group and the maize powder medium of experiment E group are all conventionally prepared into polypropylene kind pocket.
2 experimental results
After preparing the medium of each experimental group, by lucidum seed bacterium culture transferring to polypropylene kind pocket, dull and stereotyped Tip Splitting is quantitatively accessed with the card punch of sterilizing, each formula connects 2O planting bag, room temperature controls to cultivate in 25-28 DEG C, observe and measure mycelial growth rate, number of days that mycelial density, mycelium cover with medium, the results are shown in Table 1.From culture transferring to the output of results glossy ganoderma and bacterial contamination rate, the results are shown in Table 2.
Table 1 different culture media is to the growth effect of glossy ganoderma
Result shows, the growth rate of experimental group A of the present invention, B, C group is faster than the growth rate of testing D group and E group, and the number of days covering with medium is less than the number of days of experiment D group and E group.
Table 2 different culture media is on the impact of glossy ganoderma output and bacterial contamination rate
Result shows, the output of experimental group A of the present invention, B, C group is apparently higher than experiment D, E group, and difference has statistical significance (P < 0.05); The bacterial contamination rate of experiment B group is minimum, with experiment A group and C group comparatively close, there was no significant difference, combine E group with experiment D to compare, there is significant difference (P < 0.05), show that experiment B group culture medium prescription effectively can suppress the germ reproduction of glossy ganoderma matrix, effectively can reduce the probability that lucidum strain infects parasite or germ.
Claims (7)
1. a cultivation method for glossy ganoderma, is characterized in that: it comprises the following steps:
S1: the preparation of composts or fertilisers of cultivating: take composts or fertilisers of cultivating cotton seed hulls 50-300 weight portion, ramulus mori 10-80 weight portion, smilax 8-50 weight portion, silkworm excrement 8-50 weight portion, selfheal 5-30 weight portion, dayflower 5-30 weight portion by weight, for subsequent use;
S2: pulverize after the cotton seed hulls in S1 step, ramulus mori, smilax, silkworm excrement, selfheal and dayflower mixing, add distilled water and stir the rear water content measured in composts or fertilisers of cultivating, water content control, at 50-65%, then piles vexed 6-15h;
S3: loaded in choke protective bag by composts or fertilisers of cultivating after piling vexed end, pumps in choke protective bag after air, in bag, is filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 24-72h;
S4: the composts or fertilisers of cultivating in S3 step storage bag is loaded polypropylene kind pocket, then carry out sterilization treatment, lucidum strain is inoculated into polypropylene kind pocket;
The indoor maintenance of S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, relative moisture control 75-90% is dark;
S6: when glossy ganoderma cap launches, cap starts keratin, starts to play shot spore, can gather by bagging.
2. the cultivation method of a kind of glossy ganoderma according to claim 1, is characterized in that: the composts or fertilisers of cultivating in S1 step and weight proportion thereof are: cotton seed hulls 80-200 weight portion, ramulus mori 10-80 weight portion, smilax 8-50 weight portion, silkworm excrement 8-50 weight portion, selfheal 5-30 weight portion, dayflower 5-30 weight portion.
3. the cultivation method of a kind of glossy ganoderma according to claim 1, is characterized in that: the composts or fertilisers of cultivating in S1 step and weight proportion thereof are: cotton seed hulls 100-150 weight portion, ramulus mori 30-60 weight portion, smilax 12-40 weight portion, silkworm excrement 12-40 weight portion, selfheal 10-20 weight portion, dayflower 10-20 weight portion.
4. the cultivation method of a kind of glossy ganoderma according to claim 1, is characterized in that: the composts or fertilisers of cultivating in S1 step and weight proportion thereof are: cotton seed hulls 120 weight portion, ramulus mori 50 weight portion, smilax 20 weight portion, silkworm excrement 20 weight portion, selfheal 15 weight portion, dayflower 15 weight portion.
5. the cultivation method of a kind of glossy ganoderma according to claim 1, is characterized in that: the water content in S2 step composts or fertilisers of cultivating, at 55-60%, then piles vexed 8-12h.
6. the cultivation method of a kind of glossy ganoderma according to claim 1; it is characterized in that: composts or fertilisers of cultivating is loaded in choke protective bag after S3 step piles vexed end; pump in choke protective bag after air, in bag, be filled with ammonia, and then seal sack be prepared into composts or fertilisers of cultivating storage bag preserve 36-48h.
7. the cultivation method of a kind of glossy ganoderma according to claim 1, is characterized in that: S4 step postvaccinal kind of pocket traverse is in fungus shelf, and indoor temperature controls between 28-32 DEG C, relative moisture control 80-85%.
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| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
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| CN107455142A (en) * | 2017-08-29 | 2017-12-12 | 田林县群英农业有限公司 | A kind of implantation methods for improving ganoderma lucidum quality |
| CN107567950A (en) * | 2017-08-29 | 2018-01-12 | 田林县群英农业有限公司 | A kind of cultural method of ganoderma lucidum |
| CN108575549A (en) * | 2018-03-15 | 2018-09-28 | 贵州原生态生物科技有限公司 | A kind of simulated wild glossy ganoderma cultivating superior high-yield method |
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