CN104415089B - 到手香萃取物在制备具有抗微生物功效的组合物中的用途 - Google Patents
到手香萃取物在制备具有抗微生物功效的组合物中的用途 Download PDFInfo
- Publication number
- CN104415089B CN104415089B CN201410442909.2A CN201410442909A CN104415089B CN 104415089 B CN104415089 B CN 104415089B CN 201410442909 A CN201410442909 A CN 201410442909A CN 104415089 B CN104415089 B CN 104415089B
- Authority
- CN
- China
- Prior art keywords
- extract
- extraction
- column
- joss stick
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000284 extract Substances 0.000 title claims abstract description 82
- 239000000203 mixture Substances 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims description 22
- 235000004094 Coleus amboinicus Nutrition 0.000 title claims description 7
- 241001184136 Plectranthus amboinicus Species 0.000 title claims 3
- 230000000845 anti-microbial effect Effects 0.000 title abstract description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 80
- 206010000496 acne Diseases 0.000 claims abstract description 29
- 208000002874 Acne Vulgaris Diseases 0.000 claims abstract description 28
- 238000000605 extraction Methods 0.000 claims description 30
- 238000001223 reverse osmosis Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 241000186427 Cutibacterium acnes Species 0.000 claims description 9
- 229940055019 propionibacterium acne Drugs 0.000 claims description 9
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 7
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 5
- 240000001076 Osbeckia chinensis Species 0.000 claims description 4
- 241000186429 Propionibacterium Species 0.000 claims description 4
- 241000221095 Simmondsia Species 0.000 claims description 4
- 235000004433 Simmondsia californica Nutrition 0.000 claims description 4
- 238000004587 chromatography analysis Methods 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims description 4
- 229960004889 salicylic acid Drugs 0.000 claims description 4
- 230000000699 topical effect Effects 0.000 claims description 4
- 230000003110 anti-inflammatory effect Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 230000000844 anti-bacterial effect Effects 0.000 claims description 2
- 239000003599 detergent Substances 0.000 claims description 2
- 230000009036 growth inhibition Effects 0.000 claims 2
- 244000146462 Centella asiatica Species 0.000 claims 1
- 235000004032 Centella asiatica Nutrition 0.000 claims 1
- 230000002265 prevention Effects 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 abstract description 6
- 230000000813 microbial effect Effects 0.000 abstract description 6
- 208000015181 infectious disease Diseases 0.000 abstract description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 30
- 241000196324 Embryophyta Species 0.000 description 15
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 15
- 241000191967 Staphylococcus aureus Species 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 7
- 230000002757 inflammatory effect Effects 0.000 description 6
- HTRXGEPDTFSKLI-UHFFFAOYSA-N butanoic acid;ethyl acetate Chemical compound CCCC(O)=O.CCOC(C)=O HTRXGEPDTFSKLI-UHFFFAOYSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 244000119308 Coleus amboinicus Species 0.000 description 4
- 238000004440 column chromatography Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 241001646982 Thamnolia vermicularis Species 0.000 description 3
- 229940059958 centella asiatica extract Drugs 0.000 description 3
- 239000003937 drug carrier Substances 0.000 description 3
- FTVWIRXFELQLPI-ZDUSSCGKSA-N (S)-naringenin Chemical compound C1=CC(O)=CC=C1[C@H]1OC2=CC(O)=CC(O)=C2C(=O)C1 FTVWIRXFELQLPI-ZDUSSCGKSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 239000012539 chromatography resin Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000020154 Acnes Diseases 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 235000021508 Coleus Nutrition 0.000 description 1
- 244000061182 Coleus blumei Species 0.000 description 1
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Natural products O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 1
- IECPWNUMDGFDKC-UHFFFAOYSA-N Fusicsaeure Natural products C12C(O)CC3C(=C(CCC=C(C)C)C(O)=O)C(OC(C)=O)CC3(C)C1(C)CCC1C2(C)CCC(O)C1C IECPWNUMDGFDKC-UHFFFAOYSA-N 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 235000016887 Leptospermum scoparium Nutrition 0.000 description 1
- 241000120647 Osbeckia Species 0.000 description 1
- 208000012868 Overgrowth Diseases 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 206010037888 Rash pustular Diseases 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241001147693 Staphylococcus sp. Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 238000011482 antibacterial activity assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 229940063193 cleocin Drugs 0.000 description 1
- KDLRVYVGXIQJDK-AWPVFWJPSA-N clindamycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@H](C)Cl)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 KDLRVYVGXIQJDK-AWPVFWJPSA-N 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000002024 ethyl acetate extract Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 229940048400 fucidin Drugs 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000017066 negative regulation of growth Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 229940127249 oral antibiotic Drugs 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 208000029561 pustule Diseases 0.000 description 1
- 210000002374 sebum Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- HJHVQCXHVMGZNC-JCJNLNMISA-M sodium;(2z)-2-[(3r,4s,5s,8s,9s,10s,11r,13r,14s,16s)-16-acetyloxy-3,11-dihydroxy-4,8,10,14-tetramethyl-2,3,4,5,6,7,9,11,12,13,15,16-dodecahydro-1h-cyclopenta[a]phenanthren-17-ylidene]-6-methylhept-5-enoate Chemical compound [Na+].O[C@@H]([C@@H]12)C[C@H]3\C(=C(/CCC=C(C)C)C([O-])=O)[C@@H](OC(C)=O)C[C@]3(C)[C@@]2(C)CC[C@@H]2[C@]1(C)CC[C@@H](O)[C@H]2C HJHVQCXHVMGZNC-JCJNLNMISA-M 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- IIACRCGMVDHOTQ-UHFFFAOYSA-N sulfamic acid Chemical compound NS(O)(=O)=O IIACRCGMVDHOTQ-UHFFFAOYSA-N 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 229940040944 tetracyclines Drugs 0.000 description 1
- 239000006208 topical dosage form Substances 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/532—Agastache, e.g. giant hyssop
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/192—Carboxylic acids, e.g. valproic acid having aromatic groups, e.g. sulindac, 2-aryl-propionic acids, ethacrynic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/60—Salicylic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/368—Carboxylic acids; Salts or anhydrides thereof with carboxyl groups directly bound to carbon atoms of aromatic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Dermatology (AREA)
- Medical Informatics (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Birds (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Emergency Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明公开了一种到手香萃取物在制备具有抗微生物功效的组合物中的用途,其中该到手香萃取物是以体积百分比浓度为50%(含)以上的醇类溶液通过抽提方式萃取到手香植株而得。其中该组合物可用于抑制或预防由微生物感染所导致的痤疮。
Description
技术领域
本发明涉及一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为50%(含)以上的醇类溶液通过抽提方式萃取到手香植株而得。具体而言,该具有抗微生物功效的组合物可用于抑制或预防由微生物感染所导致的痤疮。
背景技术
痤疮,俗称“青春痘”,是毛囊皮脂腺发炎的疾病,85%以上的成人在成长过程中皆有发生痤疮的记录。依症状的不同,痤疮可分为非发炎型痤疮如粉刺,及发炎型痤疮如脓包或毛囊发炎。其中因皮脂过度分泌造成细菌过度生长,所引发的发炎型痤疮最为难以治愈。
目前对于细菌引起的发炎型痤疮常见的治疗方法有外用药剂及内服药剂。外用药剂主要以外用抗生素药膏或药水(Cleocin或Fucidin),可以抑制痤疮杆菌的生长,减轻发炎反应。而内服药剂如口服抗生素是痤疮治疗的第一线用药,常见的有四环霉素、红霉素或磺铵类,主要作用在减少游离性脂肪酸的生成,有抗发炎效果,并非用来杀菌。而利用抗生素来治疗细菌所引起的发炎型痤疮已经造成细菌的抗药性问题越来越严重。
因此,利用天然成分来抑制引起发炎型痤疮的细菌,而减少抗生素的使用或服用,是非常重要的。
发明内容
鉴于上述,本发明的目的在于提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物是以体积百分比浓度为50%(含)以上的醇类溶液通过抽提方式萃取到手香植株而得。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该微生物为细菌。又该细菌可为丙酸菌属(Propionibacterium sp.)属、葡萄球菌属(Staphylococcus sp.)或假单胞菌属(Pseudomonas sp.)。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该细菌为丙酸菌属(Propionibacterium sp.)属或葡萄球菌属(Staphylococcussp.)。
另一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该细菌为丙酸痤疮杆菌(Propionibacterium acnes)、金黄色葡萄球菌(Staphylococcus aureus)或绿脓杆菌(Pseudomonas aeruginosa)。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为50%(含)以上的甲醇或乙溶液通过抽提方式萃取到手香植株而得。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为50%~95%的醇类溶液通过抽提方式萃取到手香植株而得,又该50%~95%的醇类溶液可为甲醇或乙醇溶液。另一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为70%~95%的醇类溶液通过抽提方式萃取到手香植株而得,又该70%~95%的醇类溶液可为甲醇或乙醇溶液。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为50%~95%的醇类溶液通过抽提方式萃取到手香植株而得的到手香萃取物,以及以体积百分比浓度为70%~95%的醇类溶液通过抽提方式萃取到手香植株而得的手香萃取物的组合。又该50%~95%的醇类溶液可为甲醇或乙醇溶液,以及该70%~95%的醇类溶液可为甲醇或乙醇溶液。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为50%(含)以上的醇类溶液抽提后再以乙酸乙酯(Ethyl Acetate)抽提萃取到手香植株而得。又该50%(含)以上浓度的醇类溶液可为甲醇或乙醇。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为50%~95%的醇类溶液抽提后再以乙酸乙酯(Ethyl Acetate)抽提萃取到手香植株而得,又该50%~95%醇类溶液可为甲醇或乙醇溶液。另一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为70%~95%的醇类溶液抽提后再以乙酸乙酯(Ethyl Acetate)抽提萃取到手香植株而得,又该70%~95%的醇类溶液可为甲醇或乙醇溶液。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为50%~95%的醇类溶液抽提后再以乙酸乙酯(Ethyl Acetate)抽提萃取到手香植株而得的到手香萃取物,以及以体积百分比浓度为70%~95%的醇类溶液抽提后再以乙酸乙酯(Ethyl Acetate)抽提萃取到手香植株而得的到手香萃取物的组合。又该50%~95%的醇类溶液可为甲醇或乙醇溶液,以及该70%~95%的醇类溶液可为甲醇或乙醇溶液。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该组合物尚包含一适当赋形剂而制成外用药剂形式或化妆品形式或清洁剂形式。
另一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该组合物可用以治疗或预防痤疮。又该到手香萃取物可与其他抗菌剂、消炎药、清洁或护肤的组成份、或其组合制成外用药剂形式或化妆品形式或清洁剂形式。
一方面,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该组合物还包含积雪草萃取物及/或水杨酸。
本发明亦提供一种治疗或预防痤疮的组合物,其是呈外用剂型,其组合物包含如上述定义的到手香萃取物及积雪草萃取物。
根据本发明具体的实施例,该组合物更进一步包含医药上可接受的载剂。
根据本发明具体的实施例,其中含有到手香萃取物的具有抗微生物功效的组合物可用在抑制或预防由微生物感染所导致的痤疮。
因此,本发明提供一种到手香萃取物在制备具有抗微生物功效组合物中的用途,其中该到手香萃取物为以体积百分比浓度为50%(含)以上的醇类溶液通过抽提方式萃取到手香植株而得。具体而言,其中该组合物用在抑制或预防由微生物感染所导致的痤疮。
本发明的该等及其它方面,可通过以下的较佳具体实施例的描述以及图式,得以更为明晰;即便其中可能会有变化或修饰,但不背离本发明所揭露的新颖观念的精神及范畴。
具体实施方式
除非另有定义,所有本文所用的技术性及科学性术语,对于属于本发明领域的具有通常知识者而言,皆具有与其所习知者相同意义。
除非文中有清楚指明者,在本文中所使用的单数形式“一”、“一种”、及“该”的涵义均为包括“至少一种”的复数形式。因此,例如,当提及“一成分”时,包括复数个该等成分及本领域技术人员所知的同等物。
本发明组成物可调制成药品的型式。可依任何常规技术配合常用的赋形剂或填充剂。亦可依一般医药上常用的技术或方法,含有医疗有效量的本发明组成物,佐以医药上可接受的载剂调制成药品。
本文所使用的“医疗有效量”一词是指可有效治疗或预防痤疮的组合物浓度,其可依照施予的方式及治疗的情况,包括年龄、体重、症状、治疗效果、施用方式及治疗时间调整。
本文所使用的“载剂”或“医药上可接受的载剂”一词是指用在医药的稀释剂、赋形剂或类似物,包括制药业一般技术人员所熟知者。
以上述发明说明以及下列实施例说明本发明,但并非用以限制本发明的范围。
实施例1:50%~95%乙醇溶液抽提的到手香萃取物
取需要量的到手香干燥药材粉末,加入体积百分比浓度为95%的乙醇溶液萃取;将过滤所得药渣再以体积百分比浓度为95%的乙醇溶液进行第二次萃取,经过滤将两次过滤液合并,进行减压浓缩至剩下原滤液体积的约1/20,得萃取浓缩液准备进行管柱层析分离。
将所得萃取浓缩液进行管柱层析,层析树脂为HP-20。将所得萃取浓缩液加入逆渗透(RO)水,混和均匀后导入管柱中进行层析,接着以逆渗透水导入管柱抽提,收集抽提液。接着以4倍管柱体积的逆渗透水与体积百分比浓度为95%的乙醇(体积比1:1)的溶液导入管柱中抽提,收集抽提液,再进行减压浓缩至少量,冷冻干燥得一到手香萃取物,作为体积百分比浓度为0%~50%的乙醇溶液抽提对照组。
接着以4倍管柱体积的体积百分比浓度为95%的乙醇溶液导入管柱抽提,收集抽提液,再进行减压浓缩至干燥得到手香进一步的萃取物,编号为F3,即体积百分比浓度为50%~95%的乙醇溶液抽提萃取物。再以乙酸乙酯(Ethyl Acetate,EA)导入管柱抽提,收集抽提液进行减压浓缩至干燥得到手香乙酸乙酯抽提萃取物。
实施例2:70%~95%乙醇溶液抽提的到手香萃取物
依制备实施例1的步骤进行萃取得萃取浓缩液,准备进行管柱层析。将所得萃取浓缩液进行管柱层析,层析树脂为HP-20。将所得萃取浓缩液加入逆渗透(RO)水,混和均匀后导入管柱中进行层析,接着以逆渗透水导入管柱抽提,收集抽提液。接着以逆渗透水与体积百分比浓度为95%的乙醇(体积比3:7)的溶液导入管柱中抽提,收集抽提液再进行减压浓缩至少量,冷冻干燥得一到手香萃取物。接着以体积百分比浓度为95%的乙醇溶液导入管柱抽提,收集抽提液,再进行减压浓缩至干燥得一到手香进一步萃取物,编号为F4,即体积百分比浓度为70%~95%的乙醇溶液抽提萃取物。再以乙酸乙酯(Ethyl Acetate,EA)导入管柱抽提,收集抽提液进行减压浓缩至干燥为到手香乙酸乙酯抽提萃取物。
实施例3:离体抗菌实验
微生物感染在痤疮发病中扮演重要角色,其中丙酸痤疮杆菌(Propionibacteriumacnes)及金黄色葡萄球菌(Staphylococcus aureus)的影响较为严重且最为多见。本试验使用丙酸痤疮杆菌(Propionibacterium acnes)及金黄色葡萄球菌(Staphylococcusaureus),评估本发明到手香萃取物及其制备的组合物的抑菌能力。
本实验样品为到手香萃取物及其制备的组合物-痘康组合成分。其中到手香萃取物由F3(50%~95%乙醇溶液抽提萃取物)及F4(70%~95%乙醇溶液抽提萃取物)所组成。痘康组合成分除了含有F3及F4,另包含积雪草萃取物、水杨酸及其他赋形成分。将1%的实验样品添加到含待测菌株丙酸痤疮杆菌(Propionibacterium acnes)及金黄色葡萄球菌(Staphylococcus aureus)(2-8×105菌落形成单位/毫升)的测试孔中,在受控的温度及培养基条件下生长1至2天后,利用分光光度计(波长600nm)进行混浊度测定,以检查微生物生长情形。(+)表示抑制生长,(-)表示混浊/不影响微生物生长。所得结果如下表1。
表1实验样品的抑菌能力
实验样品 | Propionibacterium acnes | Staphylococcus aureus |
到手香萃取物1% | + | + |
痘康组合成分1% | + | + |
由实验结果可知本发明到手香萃取物及痘康组合成分对于痤疮致病菌丙酸痤疮杆菌(Propionibacterium acnes),及金黄色葡萄球菌(Staphylococcus aureus)具有完全抑制生长的效果。
Claims (8)
1.一种到手香萃取物在制备具有对丙酸痤疮杆菌达到完全抑制生长的功效的组合物中的用途,其中该到手香萃取物是编号为F3的到手香萃取物以及编号为F4的到手香萃取物的组合,
其中编号为F3的到手香萃取物是以下列方法制备:取到手香干燥药材粉末,加入体积百分比浓度为95%的乙醇溶液萃取;进行减压浓缩,获得萃取浓缩液;将所得萃取浓缩液加入逆渗透水,混和均匀后导入管柱中进行层析,接着以逆渗透水导入管柱抽提;接着以4倍管柱体积的逆渗透水与体积百分比浓度为95%的乙醇以体积比1:1的溶液导入管柱中抽提;以及接着以4倍管柱体积的体积百分比浓度为95%的乙醇溶液导入管柱抽提,收集抽提液,再进行减压浓缩至干燥,获得编号为F3的到手香萃取物;以及
其中编号为F4的到手香萃取物是以下列方法制备:取到手香干燥药材粉末,加入体积百分比浓度为95%的乙醇溶液萃取;进行减压浓缩,获得萃取浓缩液;将所得萃取浓缩液加入逆渗透水,混和均匀后导入管柱中进行层析,接着以逆渗透水导入管柱抽提;接着以逆渗透水与体积百分比浓度为95%的乙醇以体积比3:7的溶液导入管柱中抽提,接着以体积百分比浓度为95%的乙醇溶液导入管柱抽提,收集抽提液,再进行减压浓缩至干燥,获得编号为F4的到手香萃取物。
2.如权利要求1所述的用途,其中该到手香萃取物是以1%浓度制得该组合物达对丙酸痤疮杆菌完全抑制生长的功效。
3.如权利要求1或2所述的用途,其中该组合物还包含一适当赋形剂而制成外用药剂形式或化妆品形式或清洁剂形式。
4.如权利要求1或2所述的用途,其中该组合物可用以治疗或预防痤疮。
5.如权利要求1或2所述的用途,其中该到手香萃取物可与其他抗菌剂、消炎药、清洁或护肤的组成份、或其组合制成外用药剂形式或化妆品形式或清洁剂形式。
6.如权利要求1或2所述的用途,其中该组合物还包含积雪草萃取物。
7.如权利要求1或2所述的用途,其中该组合物还包含水杨酸。
8.如权利要求6所述的用途,其中该组合物还包含水杨酸。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW102131723 | 2013-09-03 | ||
TW102131723A TWI663982B (zh) | 2013-09-03 | 2013-09-03 | 到手香萃取物用於製備具抗微生物功效組合物之用途 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104415089A CN104415089A (zh) | 2015-03-18 |
CN104415089B true CN104415089B (zh) | 2021-04-20 |
Family
ID=52668165
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410442909.2A Active CN104415089B (zh) | 2013-09-03 | 2014-09-02 | 到手香萃取物在制备具有抗微生物功效的组合物中的用途 |
Country Status (8)
Country | Link |
---|---|
US (1) | US20150079202A1 (zh) |
JP (2) | JP2015091772A (zh) |
KR (1) | KR20150027009A (zh) |
CN (1) | CN104415089B (zh) |
HK (1) | HK1208149A1 (zh) |
IN (1) | IN2014DE02523A (zh) |
MY (1) | MY180113A (zh) |
TW (1) | TWI663982B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RS64356B1 (sr) * | 2016-07-17 | 2023-08-31 | Oneness Biotech Co Ltd | Topikalna formulacija za pospešivanje zarastanja rana |
EP3893908A1 (en) * | 2018-12-10 | 2021-10-20 | University of Pretoria | Anti-acne pharmaceutical compositions |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101375933A (zh) * | 2007-08-29 | 2009-03-04 | 财团法人生物技术开发中心 | 制备用于治疗皮肤病症和促进伤口愈合的植物提取物的方法 |
CN103127215A (zh) * | 2011-11-22 | 2013-06-05 | 合一生技股份有限公司 | 具有抗关节炎活性的到手香萃取物 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04217906A (ja) * | 1990-01-19 | 1992-08-07 | Shiseido Co Ltd | 皮膚外用剤 |
DE19654635C1 (de) * | 1996-12-28 | 1998-01-08 | Singh Verma Shyam B | Kosmetische Zubereitungen enthaltend Extrakte von Phyllanthus emblica und Centella asiatica |
WO2001080827A2 (en) * | 2000-04-21 | 2001-11-01 | Nature's Cure | Salicylic acid acne spray formulations and methods for treating acne with same |
JP4589483B2 (ja) * | 2000-05-12 | 2010-12-01 | 花王株式会社 | ニキビ予防治療剤 |
GB0403702D0 (en) * | 2004-02-19 | 2004-03-24 | Boots Co Plc | Skincare compositions |
TWI361074B (en) * | 2005-12-22 | 2012-04-01 | Dev Center Biotechnology | Plant extracts for enhancing healing of wounds,and the preparation process and use thereof |
WO2012131348A1 (en) * | 2011-03-31 | 2012-10-04 | Evocutis Plc | Salicylic acid topical formulation |
-
2013
- 2013-09-03 TW TW102131723A patent/TWI663982B/zh active
-
2014
- 2014-09-02 CN CN201410442909.2A patent/CN104415089B/zh active Active
- 2014-09-03 JP JP2014179539A patent/JP2015091772A/ja active Pending
- 2014-09-03 IN IN2523DE2014 patent/IN2014DE02523A/en unknown
- 2014-09-03 US US14/475,888 patent/US20150079202A1/en not_active Abandoned
- 2014-09-03 KR KR20140116965A patent/KR20150027009A/ko not_active Application Discontinuation
- 2014-09-03 MY MYPI2014002547A patent/MY180113A/en unknown
-
2015
- 2015-08-18 HK HK15107974.8A patent/HK1208149A1/zh unknown
-
2019
- 2019-07-18 JP JP2019132598A patent/JP2019178170A/ja not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101375933A (zh) * | 2007-08-29 | 2009-03-04 | 财团法人生物技术开发中心 | 制备用于治疗皮肤病症和促进伤口愈合的植物提取物的方法 |
CN103127215A (zh) * | 2011-11-22 | 2013-06-05 | 合一生技股份有限公司 | 具有抗关节炎活性的到手香萃取物 |
Non-Patent Citations (3)
Title |
---|
乌梅、藿香、甘草抗菌作用的初步研究;陈士英;《湖北农业科学》;19930630(第6期);16页右栏第4段,表1 * |
到手香水提液体外抗菌活性研究;王玲等;《时珍国医国药》;20130821;第24卷(第7期);摘要 * |
陈士英.乌梅、藿香、甘草抗菌作用的初步研究.《湖北农业科学》.1993,(第6期), * |
Also Published As
Publication number | Publication date |
---|---|
TW201509428A (zh) | 2015-03-16 |
JP2015091772A (ja) | 2015-05-14 |
HK1208149A1 (zh) | 2016-02-26 |
IN2014DE02523A (zh) | 2015-06-26 |
TWI663982B (zh) | 2019-07-01 |
MY180113A (en) | 2020-11-23 |
KR20150027009A (ko) | 2015-03-11 |
CN104415089A (zh) | 2015-03-18 |
JP2019178170A (ja) | 2019-10-17 |
US20150079202A1 (en) | 2015-03-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR101135172B1 (ko) | 황백, 어성초, 선학초, 작약 및 감초의 혼합추출물을 유효성분으로 함유하는 여드름 예방, 개선 또는 치료용 조성물 | |
RU2358750C2 (ru) | Фармацевтические композиции на основе лишайника бородатого (usnea barbata) и зверобоя обыкновенного (hypericum perforatum) и их применение | |
WO2008140200A1 (en) | External compositions for the skin | |
JP4547388B2 (ja) | リグナン系化合物を利用したニキビ治療方法及び組成物(methodandcompositionfortreatingacneusinglignancompounds) | |
CN110035797B (zh) | 用于治疗痤疮的具有益生元特性的协同草药组合物 | |
KR102326886B1 (ko) | 어성초 복합 추출물을 유효성분으로 포함하는 항여드름 및 항염증 조성물 | |
CN106177100B (zh) | 一种具有祛痘作用的组合物及其应用 | |
Coopoosamy et al. | Traditional use, antibacterial activity and antifungal activity of crude extract of Aloe excelsa | |
Kim et al. | Antibacterial and anti-inflammatory effects of Jeju medicinal plants against acne-inducing bacteria | |
KR100973221B1 (ko) | 판두라틴 에이 또는 그의 유도체의 신규한 용도 | |
KR102148808B1 (ko) | 천연 항균제를 포함하는 여성청결제 조성물 | |
CN104415089B (zh) | 到手香萃取物在制备具有抗微生物功效的组合物中的用途 | |
CN114848721B (zh) | 一种含植物提取物的祛痘组合物及其应用 | |
Taqa et al. | Evaluation of anti-bacterial and efficacy of plant extract (Urtica urens) on skin wound healing in rabbit | |
CN115414308A (zh) | 一种祛痘组合物及制备方法和应用 | |
Gopalakrishnan | Insignificant antifungal activity of plant extracts on Malassezia furfur | |
JP2010202604A (ja) | 皮膚常在菌正常化剤、皮膚常在菌正常化方法及びそれを用いる皮膚外用剤又は化粧料、並びにそれらの製造方法 | |
CN111419940B (zh) | 一种祛痘的中草药组合物 | |
KR20210020817A (ko) | 망고스틴 추출물을 포함하는 여드름 원인균에 대한 항균용 조성물 및 이를 포함하는 여드름의 예방 또는 치료용 제제 | |
Mittal et al. | Effect of three herbal extracts against Streptococcus mutansobacillus acidophilus in comparison to Chlorhexidine | |
CN110897950A (zh) | 一种具有抗炎保湿和美白功效的面膜及其制备方法 | |
US9980998B2 (en) | Medicinal composition having antibiotic, anti-inflammatory, and wound healing activity | |
KR101574591B1 (ko) | 신곡 추출물을 포함하는 상처 치료용 조성물 | |
Samieerad et al. | Physicochemical and antibacterial assessment of Iranian propolis | |
Aparna et al. | Preparation of a Common Herbal Medicine with Culinary Plants for Skin Infections Caused by Candida albicans AND Propionibacterium acnes |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1208149 Country of ref document: HK |
|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |