CN104403136A - Medical pectin/bacterial cellulose composite film and preparation method thereof - Google Patents
Medical pectin/bacterial cellulose composite film and preparation method thereof Download PDFInfo
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- CN104403136A CN104403136A CN201410691819.7A CN201410691819A CN104403136A CN 104403136 A CN104403136 A CN 104403136A CN 201410691819 A CN201410691819 A CN 201410691819A CN 104403136 A CN104403136 A CN 104403136A
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Abstract
The invention provides a medical pectin/bacterial cellulose composite film and a preparation method thereof. The composite film is formed by bacterial cellulose and pectin in a in-situ composite manner, wherein the mass ratio of bacterial cellulose to pectin is (0.35-0.55):1. The preparation method comprises the following steps: (1) placing a bacterial cellulose film in NaOH aqueous solution, heating for boiling, washing, performing vacuum drying, grinding to be powdery, and placing the powder in an emulsifying machine to form bacterial cellulose emulsion; (2) adding the prepared bacterial cellulose emulsion into pectin solution, adding a cross-linking agent, uniformly stirring, leaving to stand to defoam, pouring into a forming die, freezing, unfreezing at room temperature, and performing freezing and unfreezing for three times to obtain the medical pectin/bacterial cellulose composite film. The medical pectin/bacterial cellulose composite film, provided by the invention, has the transparency of greater than 29%, lower porosity and water vapor permeability, as well as excellent sealing property, is simple in production process, safe and non-poisonous, and has a great application prospect in the field of medicines.
Description
Technical field
The present invention relates to polymeric material field, be specifically related to a kind of pectin/bacteria cellulose medical composite film and preparation method thereof.
Background technology
Bacteria cellulose is the porousness mesh nano level biopolymer synthesized by fermentable.It is the straight-chain molecule be combined into by β-Isosorbide-5-Nitrae-glycosidic link by β-D-Glucose, is therefore also called β-Isosorbide-5-Nitrae-dextran.Because it is synthesized and called after bacteria cellulose by bacterium, it is found by British scientist Brown the earliest, and be defined as cellulose substances through physics and chemistry methods analyst, be hydrolyzed to this material, hydrolyzed solution main component is glucose.It is made up of the filamentary fibers of uniqueness, and Fibre diameter is between 0.01 ~ 0.10 μm, and 2 ~ 3 orders of magnitude less of plant cellulose (10 μm), each filamentary fibers forms reticulated structure by the ultra-microfibers of some amount.The essential difference of it and plant cellulose is the former not containing hemicellulose, xylogen etc.
The Pseudomonas producing bacteria cellulose has acetobacter, rhizobium, Sarcina, Rhodopseudomonas, Azotobacter, aerobacter and Alcaligenes etc.Wherein acetobacter xylinum finds the earliest, is also to study comparatively thorough Mierocrystalline cellulose producing bacterial strain, and this bacterium can utilize multiple substrate to grow, and is the microorganism strains that current known synthetic cellulose ability is the strongest.
Bacteria cellulose, as a kind of novel nano-material, has been applied to the every field such as weaving, medical material, food, has now become international study hotspot.
The apertured polymeric film pore size distribution be made up of bacteria cellulose is at present uneven, and the size of micropore and distribution can have influence on the porosity of apertured polymeric film, ventilation property and pick up, and water retainability is also not ideal enough.
Summary of the invention
Based on above-mentioned information, the object of the present invention is to provide a kind of even aperture distribution, pectin/bacteria cellulose medical composite film of good seal performance and preparation method thereof.
Technical scheme of the present invention is as follows:
A kind of pectin/bacteria cellulose medical composite film, be composited by bacteria cellulose and pectin isl-situ, wherein, the mass ratio of bacteria cellulose and pectin is 0.35 ~ 0.55:1.
The preparation method of pectin/bacteria cellulose medical composite film, comprises the steps:
(1) bacteria cellulose film is placed in the NaOH aqueous solution, heated and boiled 30 ~ 60min, with deionized water wash to neutral, vacuum-drying, then grinds to form powdery, is placed in mulser, forms bacteria cellulose emulsion, for subsequent use;
(2) the bacteria cellulose emulsion that step (1) is obtained is added in pectin solution, and add linking agent, stir, standing and defoaming, then pour in shaping dies, freezing 12 ~ 20h, be then placed in thawed at room temperature 12 ~ 20h, freezing and thaw all circulate three times, obtain pectin/bacteria cellulose medical composite film.
In step (1), the concentration of the NaOH aqueous solution is 1 ~ 5wt%.
In step (2), the concentration of pectin solution is 4 ~ 10wt%, and solvent is water.
In step (2), described linking agent is citric acid.
In step (2), shaping dies is glass wares or ceramic utensil.
In step (2), freezing temperature is-18 ~-25 DEG C.
Pectin provided by the invention/bacteria cellulose medical composite film transparency is all greater than 29%, and porosity and water vapor transmission rate (WVTR) are all lower, favorable sealing property, and production process is simple, and safety non-toxic, has very large application prospect in field of medicaments.
Embodiment
Embodiment 1
350g bacteria cellulose film is placed in the NaOH aqueous solution that concentration is 1wt%, heated and boiled 30min, with deionized water wash to neutral, at 60 DEG C, vacuum-drying 12h, then grinds to form powdery, is placed in mulser, rotating speed is 10000r/min, time 120s, forms bacteria cellulose emulsion, for subsequent use;
Obtained bacterial cellulose powder being added to the concentration being dissolved with 1kg pectin is in the aqueous solution of 4wt%, and adds catalytic amount citric acid, stirs, standing and defoaming.
Pour in glass forming die by the above-mentioned pectin solution being added with bacteria cellulose, freezing 20h at-18 DEG C, be then placed in thawed at room temperature 20h, freezing and all circulations three times of thawing, obtain pectin/bacteria cellulose medical composite film.
Embodiment 2
400g bacteria cellulose film is placed in the NaOH aqueous solution that concentration is 2wt%, heated and boiled 40min, with deionized water wash to neutral, at 60 DEG C, vacuum-drying 14h, then grinds to form powdery, is placed in mulser, rotating speed is 10000r/min, time 120s, forms bacteria cellulose emulsion, for subsequent use;
Obtained bacterial cellulose powder being added to the concentration being dissolved with 1kg pectin is in the aqueous solution of 5wt%, and adds catalytic amount citric acid, stirs, standing and defoaming.
Pour in glass forming die by the above-mentioned pectin solution being added with bacteria cellulose, freezing 18h at-19 DEG C, be then placed in thawed at room temperature 18h, freezing and all circulations three times of thawing, obtain pectin/bacteria cellulose medical composite film.
Embodiment 3
450g bacteria cellulose film is placed in the NaOH aqueous solution that concentration is 3wt%, heated and boiled 40min, with deionized water wash to neutral, at 60 DEG C, vacuum-drying 16h, then grinds to form powdery, is placed in mulser, rotating speed is 10000r/min, time 120s, forms bacteria cellulose emulsion, for subsequent use;
Obtained bacterial cellulose powder being added to the concentration being dissolved with 1kg pectin is in the aqueous solution of 6wt%, and adds catalytic amount citric acid, stirs, standing and defoaming.
Pour in glass forming die by the above-mentioned pectin solution being added with bacteria cellulose, freezing 16h at-21 DEG C, be then placed in thawed at room temperature 16h, freezing and all circulations three times of thawing, obtain pectin/bacteria cellulose medical composite film.
Embodiment 4
500g bacteria cellulose film is placed in the NaOH aqueous solution that concentration is 4wt%, heated and boiled 50min, with deionized water wash to neutral, at 60 DEG C, vacuum-drying 18h, then grinds to form powdery, is placed in mulser, rotating speed is 10000r/min, time 120s, forms bacteria cellulose emulsion, for subsequent use;
Obtained bacterial cellulose powder being added to the concentration being dissolved with 1kg pectin is in the aqueous solution of 8wt%, and adds catalytic amount citric acid, stirs, standing and defoaming.
Pour in ceramics forming mould by the above-mentioned pectin solution being added with bacteria cellulose, freezing 14h at-23 DEG C, is then placed in thawed at room temperature 14h, and freezing and all circulations three times of thawing, obtain pectin/bacteria cellulose medical composite film.
Embodiment 5
550g bacteria cellulose film is placed in the NaOH aqueous solution that concentration is 5wt%, heated and boiled 60min, with deionized water wash to neutral, at 60 DEG C, vacuum-drying 20h, then grinds to form powdery, is placed in mulser, rotating speed is 10000r/min, time 120s, forms bacteria cellulose emulsion, for subsequent use;
Obtained bacterial cellulose powder being added to the concentration being dissolved with 1kg pectin is in the aqueous solution of 10wt%, and adds catalytic amount citric acid, stirs, standing and defoaming.
Pour in ceramics forming mould by the above-mentioned pectin solution being added with bacteria cellulose, freezing 12h at-25 DEG C, is then placed in thawed at room temperature 12h, and freezing and all circulations three times of thawing, obtain pectin/bacteria cellulose medical composite film.
Performance test:
Measure the permeability of pectin/bacteria cellulose medical composite film prepared by the embodiment of the present invention 1 ~ 5 respectively, comprise transparency, porosity and water vapor transmission rate (WVTR), result is as shown in table 1.
Transparency measures: adopt Beijing Pu Xi universal apparatus company limited UV-1950 ultraviolet spectrophotometer to analyze, film sample to be measured is cut into the rectangle of 10mm × 50mm, be affixed on inside cuvette, with empty cuvette in contrast, measure transmittance at 700 nm, by the transparency of transmittance size secondary indication film.
The mensuration of porosity: the composite films of certain mass is placed in the ethanol that volume is V1, deaeration, the cumulative volume of composite membrane and ethanol is designated as V2, then (V2-V1) is the volume of composite membrane.Composite membrane containing ethanol is taken out, remaining ethanol contend is V3, the volume of volume (V1-V3) shared by composite membrane space of ethanol contained by then in composite membrane, the cumulative volume of composite membrane is V=(V2-V1)+(V1-V3)=V2-V3.Porosity=(V1-V3)/(V2-V3).
Water vapor transmission rate (WVTR): composite films is cut into the disk that diameter is 50mm, closely covers on weighing bottle (40mm × 25mm), places a certain amount of silica gel in bottle, then weighing bottle is put into the moisture eliminator that bottom fills saturated NaCl.Moisture eliminator is placed in the environment that relative humidity is 65%, temperature is 25 DEG C.Every 4h, measure the weight of a weighing bottle, altogether 12h.Water vapor transmission rate (WVTR)=weighing bottle is of poor quality/and the useful area of composite membrane (interval time ×).
As seen from the above table, the transparency of pectin of the present invention/bacteria cellulose medical composite film is all greater than 29%, and porosity and water vapor transmission rate (WVTR) are all lower, favorable sealing property.
Although embodiment of the present invention are open as above, but it is not restricted to listed in specification sheets and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (7)
1. pectin/bacteria cellulose medical composite film, is characterized in that, is composited by bacteria cellulose and pectin isl-situ, and wherein, the mass ratio of bacteria cellulose and pectin is 0.35 ~ 0.55:1.
2. the preparation method of pectin described in claim 1/bacteria cellulose medical composite film, is characterized in that, comprise the steps:
(1) bacteria cellulose film is placed in the NaOH aqueous solution, heated and boiled 30 ~ 60min, with deionized water wash to neutral, vacuum-drying, then grinds to form powdery, is placed in mulser, forms bacteria cellulose emulsion, for subsequent use;
(2) the bacteria cellulose emulsion that step (1) is obtained is added in pectin solution, and add linking agent, stir, standing and defoaming, then pour in shaping dies, freezing 12 ~ 20h, be then placed in thawed at room temperature 12 ~ 20h, freezing and thaw all circulate three times, obtain pectin/bacteria cellulose medical composite film.
3. the preparation method of a kind of pectin/bacteria cellulose medical composite film according to claim 2, is characterized in that, in step (1), the concentration of the NaOH aqueous solution is 1 ~ 5wt%.
4. the preparation method of a kind of pectin/bacteria cellulose medical composite film according to claim 2, is characterized in that, in step (2), the concentration of pectin solution is 4 ~ 10wt%, and solvent is water.
5. the preparation method of a kind of pectin/bacteria cellulose medical composite film according to claim 2, is characterized in that, in step (2), described linking agent is citric acid.
6. the preparation method of a kind of pectin/bacteria cellulose medical composite film according to claim 2, is characterized in that, in step (2), shaping dies is glass wares or ceramic utensil.
7. the preparation method of a kind of pectin/bacteria cellulose medical composite film according to claim 2, is characterized in that, in step (2), freezing temperature is-18 ~-25 DEG C.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105688258A (en) * | 2016-03-09 | 2016-06-22 | 苏州市贝克生物科技有限公司 | Pectin and bacterial cellulose composite medical dressing and preparation method thereof |
| CN110326827A (en) * | 2019-08-06 | 2019-10-15 | 江苏万鸿健康科技有限公司 | A kind of health care chest pad for preventing and treating the proliferation of mammary gland |
| CN111135336A (en) * | 2018-11-06 | 2020-05-12 | 钟宇光 | Pectin/biological cellulose composite membrane prepared by biological fermentation of whole citrus fruits |
| CN113441015A (en) * | 2021-06-02 | 2021-09-28 | 内蒙古科技大学 | Microbial cellulose-agarose composite hydrogel-based oil-water separation membrane and preparation method thereof |
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| CN1668646A (en) * | 2002-07-12 | 2005-09-14 | 旭化成株式会社 | Water-dispersible cellulose and process for producing the same |
| CN101215388A (en) * | 2008-01-15 | 2008-07-09 | 东华大学 | Method for preparing bacteria cellulose composite membrane |
| EP2650413A1 (en) * | 2012-04-13 | 2013-10-16 | Carl Freudenberg KG | Hydrogelling fibres and fibre structures |
| CN103980526A (en) * | 2014-05-27 | 2014-08-13 | 哈尔滨工业大学 | Method for preparing acetylated-modified bacterial cellulose aerogel oil-absorbent material |
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2014
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1668646A (en) * | 2002-07-12 | 2005-09-14 | 旭化成株式会社 | Water-dispersible cellulose and process for producing the same |
| CN101215388A (en) * | 2008-01-15 | 2008-07-09 | 东华大学 | Method for preparing bacteria cellulose composite membrane |
| EP2650413A1 (en) * | 2012-04-13 | 2013-10-16 | Carl Freudenberg KG | Hydrogelling fibres and fibre structures |
| CN103980526A (en) * | 2014-05-27 | 2014-08-13 | 哈尔滨工业大学 | Method for preparing acetylated-modified bacterial cellulose aerogel oil-absorbent material |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105688258A (en) * | 2016-03-09 | 2016-06-22 | 苏州市贝克生物科技有限公司 | Pectin and bacterial cellulose composite medical dressing and preparation method thereof |
| CN111135336A (en) * | 2018-11-06 | 2020-05-12 | 钟宇光 | Pectin/biological cellulose composite membrane prepared by biological fermentation of whole citrus fruits |
| CN110326827A (en) * | 2019-08-06 | 2019-10-15 | 江苏万鸿健康科技有限公司 | A kind of health care chest pad for preventing and treating the proliferation of mammary gland |
| CN113441015A (en) * | 2021-06-02 | 2021-09-28 | 内蒙古科技大学 | Microbial cellulose-agarose composite hydrogel-based oil-water separation membrane and preparation method thereof |
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