CN104396571A - High-cordycepin-content rich-selenium cordyceps sinensis cultivation method - Google Patents

High-cordycepin-content rich-selenium cordyceps sinensis cultivation method Download PDF

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CN104396571A
CN104396571A CN201410775786.4A CN201410775786A CN104396571A CN 104396571 A CN104396571 A CN 104396571A CN 201410775786 A CN201410775786 A CN 201410775786A CN 104396571 A CN104396571 A CN 104396571A
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selenium
cordycepin
medium
cordyceps
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CN104396571B (en
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梁金权
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CHONGQING ZONGYI PHARMACEUTICAL Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms

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Abstract

The invention discloses a high-cordycepin-content rich-selenium cordyceps sinensis cultivation method. The high-cordycepin-content rich-selenium cordyceps sinensis cultivation method comprises the following steps of 1 cordyceps taishanensis primary solid cultivation, 2 cordyceps taishanensis secondary plant-expanding liquid cultivation and 3 cordyceps sinensis solid productive cultivation. The high-cordycepin-content rich-selenium cordyceps sinensis cultivation method is suitable for artificial cultivation of cordyceps sinensis and cordyceps militaris, obtained cordyceps sinensis is high in cordycepin content and selenium content, the cordycepin content can be 100-200 times of the cordycepin content of wild cordyceps sinensis, the selenium content can reach the selenium content level of the wild cordyceps sinensis, and the effective constituents and effect of the artificially-cultivated cordyceps sinensis are greatly improved. In addition, the high-cordycepin-content rich-selenium cordyceps sinensis cultivation method is high in sundry fungus resisting capacity and yield and has good economic benefit, and the artificial cultivation time can be shortened.

Description

High cordycepin selenium-rich cordyceps cultivating method
Technical field
The invention belongs to biological field, relate to a kind of high cordycepin selenium-rich cordyceps cultivating method.
Background technology
Chinese caterpillar fungus divides Cordyceps sinensis and Cordceps militaris two kinds, and both are equal not of the same race.Cordyceps sinensis is the compound that Clavicipitaceae Cordyceps Militaris parasitizes stroma on bat moth larvae body and larva body.Cordceps militaris is the abbreviation of northern CORDYCEPS, is also Cordyceps militaris or the Chinese caterpillar fungus that leaps up, pupa grass, and the old grass of popular name is worm, the medicinal fungi that bacterium combines, modern Valuable Herbal Medicine, northern Chinese caterpillar Fungus belongs to Eumycota, Ascomycetes, Hypocreales, Clavicipitaceae, Cordyceps, primary growth is at the northern area of China.Chinese caterpillar fungus has wild and cultivation two kinds.The active ingredient played a role to human body is cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide, superoxide dismutase.There is kidney tonifying benefit lung, hemostasis and phlegm, anticancer, improve immunity, antifatigue, the several functions such as protection lung, kidney, liver.Deeply welcome by many people, be called the soft gold in health products.
The effective ingredient of Cordyceps sinensis and Cordceps militaris and effect basic simlarity, cordycepin is its important composition, and that now commercially cultivates is mostly Cordceps militaris.Although cultivation improves entity output, ignore the content improving cordycepin.The Se content of wild Chinese caterpillar fungus is three times of Chinese herbal medicine astragalus, reaches 0.44mg/kg, meets the selenium-enriched food requirement that the Ministry of Public Health specifies.Also do not have the cultivation method of Chinese caterpillar fungus that the Se content of Chinese caterpillar fungus can be made to reach the Se content level of wild Chinese caterpillar fungus now.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, provide a kind of cordycepin and Se content is high, the Chinese caterpillar fungus cultivating method of resistance.
The object of the present invention is achieved like this: a kind of high cordycepin selenium-rich cordyceps cultivating method, comprises the steps:
1) Cordyceps Militaris one-level solid culture:
1. inclined-plane solid culture medium is prepared: medium component proportioning is: potato 100-140g, glucose 10-14g, beef extract 4-6g, KH 2pO 40.4-0.6g, MgSO 40.2-0.3g, selenium methionine 0.008-0.012g, agar 8-12g; First boiled by potato, mash, add all the other compositions aforementioned, heating is boiled and is allowed agar melt completely, and adding distil water is to 500ml, and stir dress inclined-plane processed, and sterilizing is chilled to room temperature and prepares inoculation;
2. inoculate, cultivate: the mycelium aseptically inoculating Chinese caterpillar fungus with inclined-plane solid culture medium prepared by step 1, lucifuge 24 DEG C cultivates 3-4 days, then cultivates 4-5 days at 20 DEG C, then 18 ~ 22 DEG C of natural lightings 24 hours, stand-by;
2) Cordyceps Militaris secondary expanding species liquid culture:
1. secondary liquid medium is prepared: with distilled water obtaining liq medium, each constituent content is: peptone 13-17g/L, glucose 18-22g/L, KH 2pO 40.8-1.2g/L, 7H 2oMgSO 40.4-0.6g/L, VB 18-12mg/L, selenium methionine 0.04-0.06g/L, sterilizing is stand-by;
2. inoculate, cultivate: aseptically inoculate with liquid nutrient medium prepared by step 1 mycelium that aforementioned one-level solid culture obtains, lucifuge 20 ~ 24 DEG C, shaking table is cultivated, and completes secondary expanding species liquid culture when there is small rice grain sample mycelium pellet;
3) Chinese caterpillar fungus solids manufacture is cultivated:
1. solids manufacture medium is prepared: medium component proportioning is: rice 800-1200g, distilled water 1100-1300g, peptone 10-14g, glucose 16-20g, KH 2pO 41.5-2.5g, 7H 2oMgSO 40.8-1.2g, selenium methionine 0.4-0.6g, VB 11.5-2.5mg; First each composition distilled water except rice is dissolved preparation and become nutrient solution, then rice and nutrient solution are loaded in proportion in blake bottle, sealing, steams to rice ripe, is cooled to room temperature stand-by;
2. inoculate, cultivate: aseptically inoculate with solid culture medium prepared by step 1 culture fluid that said second expanding species liquid culture obtains; Put into and cultivate the lucifuge cultivation of 18 ~ 23 DEG C, darkroom; Put into natural daylight or artificial lighting when media surface grows white hypha, 18 ~ 23 DEG C of ambient growth, when stroma is long neat, tip is expanded, sporulation time can gather.
In described step 2 Cordyceps Militaris secondary expanding species liquid culture, after inoculation, shaking table is cultivated is that 130 ~ 150 turns/min shaking table cultivates 48 hours.
In Cordyceps Militaris secondary expanding species liquid culture step in described step 2, the mycelium 3-3.5g that every 200ml liquid nutrient medium inoculation one-level solid culture obtains.
In described step 3, Chinese caterpillar fungus solids manufacture is cultivated, and rice and nutrient solution are by weight in the ratio loading blake bottle of 1:1.2, and sealing, 95-100 DEG C is steamed 30-40 minute, be cooled to room temperature stand-by.
In described step 3, Chinese caterpillar fungus solids manufacture is cultivated, the culture fluid obtained by solid culture medium inoculation secondary expanding species liquid culture, and inoculum concentration is every 100g rice inoculation 0.5ml culture fluid.
In described step 3, Chinese caterpillar fungus solids manufacture is cultivated, and puts into and cultivate 18 ~ 23 DEG C, darkroom lucifuge cultivation 8-10 days after inoculation.
The invention has the beneficial effects as follows: provide a kind of low cost, high production, the cultivating method of the Chinese caterpillar fungus of high, the rich selenium of cordycepin content can be turned out.The present invention is applicable to the artificial cultivation of Cordyceps sinensis and Cordceps militaris, and the Chinese caterpillar fungus obtained is high containing cordycepin, can reach more than 100-200 times of wild Chinese caterpillar fungus; Se content is high, can reach the level of wild Chinese caterpillar fungus, and drastically increase active ingredient and effect of cultivation Chinese caterpillar fungus, and cultivate the anti-miscellaneous bacteria power of Cordyceps Militaris by force by this method, output is high, can shorten the cultivation time, has good economic benefit.
Embodiment
The cultivating method of the rich selenium Cordyceps sinensis of embodiment 1 high cordycepin
Cultivate the method for the rich selenium Cordyceps sinensis of high cordycepin, operate in accordance with the following steps:
1) Cordyceps Militaris one-level solid culture:
1. inclined-plane solid culture medium is prepared: medium component proportioning is: potato 120g, glucose 12g, beef extract 5g, KH 2pO 40.5g, MgSO 40.25g, selenium methionine 0.01g, agar 10g; Potato first boils, and mashes, and adds all the other compositions aforementioned, and heating is boiled and allowed agar melt completely, and adding distil water, to 500ml, stirs, and gets the test tube of 40ml, and often pipe loads medium 7ml, bevel, high pressure steam sterilization, is cooled to room temperature and prepares inoculation;
2. inoculate, cultivate: the mycelium 3mm aseptically inoculating Cordyceps sinensis with inclined-plane solid culture medium prepared by step 1 2, lucifuge 24 DEG C is cultivated 3 days, then cultivates 4 days at 20 DEG C, then 18 ~ 22 DEG C of natural lightings 24 hours, completes activation.
2) Cordyceps Militaris secondary expanding species liquid culture:
1. secondary liquid medium is prepared: with distilled water obtaining liq medium, each constituent content is: peptone 15g/L, glucose 20g/L, KH 2pO 41g/L, 7H 2oMgSO 40.5g/L, VB 110mg/L, selenium methionine 0.05g/L, recording pH value is 7.0, and sterilizing is stand-by;
2. inoculate, cultivate: aseptically inoculate with liquid nutrient medium prepared by step 1 mycelium that aforementioned one-level solid culture obtains, every 200ml liquid nutrient medium inoculation mycelium 3g, lucifuge 20 ~ 24 DEG C, 130 ~ 150 turns/min shaking table cultivates 48 hours, completes secondary expanding species liquid culture when there is small rice grain sample mycelium pellet;
3) Chinese caterpillar fungus solids manufacture is cultivated:
1. solids manufacture medium is prepared: medium component proportioning is: rice 1000g, distilled water 1200g, peptone 12g, glucose 18g, KH 2pO 42g, 7H 2oMgSO 41g, selenium methionine 0.5g, VB 12mg, recording pH value is 6.5; First each composition distilled water except rice is dissolved and become nutrient solution, again rice and nutrient solution are loaded blake bottle by weight the ratio of 1:1.2, load 50 grams of rice, 60 grams of nutrient solutions in the blake bottle of each 500ml, sealing, 95-100 DEG C is steamed 30 minutes, is cooled to room temperature stand-by;
2. inoculate, cultivate: aseptically inoculate with solid culture medium prepared by step 1 culture fluid that said second expanding species liquid culture obtains, every 100g rice inoculation 0.5ml culture fluid; Put into and cultivate 18 ~ 23 DEG C, darkroom lucifuge cultivation 8-10 days; Put into natural daylight or artificial lighting when media surface grows white hypha, 18 ~ 23 DEG C of ambient growth 40-55 days, when stroma is long neat, tip is expanded, sporulation time can gather.
Utilizing the method in Chinese Pharmacopoeia to record in the Cordyceps sinensis nurtured cordycepin content in every 100g is 217.63mg; Recording Se content is 0.327mg/kg, reaches the Se content level of wild Chinese caterpillar fungus.
The cultivating method of the rich selenium Cordceps militaris of embodiment 2 high cordycepin
Cultivate the method for the rich selenium Cordceps militaris of high cordycepin, operate in accordance with the following steps:
1) Cordyceps Militaris one-level solid culture:
1. inclined-plane solid culture medium is prepared: medium component proportioning is: potato 110g, glucose 11g, beef extract 4.5g, KH 2pO 40.4g, MgSO 40.2g, selenium methionine 0.009g, agar 9g; Potato first boils, and mashes, and adds all the other compositions aforementioned, and heating is boiled and allowed agar melt completely, and adding distil water, to 500ml, stirs, and gets the test tube of 40ml, and often pipe loads medium 8ml, bevel, high pressure steam sterilization, is cooled to room temperature and prepares inoculation;
2. inoculate, cultivate: the mycelium 3mm aseptically inoculating Cordceps militaris with inclined-plane solid culture medium prepared by step 1 2, lucifuge 24 DEG C is cultivated 3 days, then cultivates 4 days at 20 DEG C, then 18 ~ 22 DEG C of natural lightings 24 hours, completes activation.
2) Cordyceps Militaris secondary expanding species liquid culture:
1. secondary liquid medium is prepared: with distilled water obtaining liq medium, each constituent content is: peptone 14g/L, glucose 18g/L, KH 2pO 40.9g/L, 7H 2oMgSO 40.4g/L, VB 19mg/L, selenium methionine 0.04g/L, recording pH value is 7.1, and sterilizing is stand-by;
2. inoculate, cultivate: aseptically inoculate with liquid nutrient medium prepared by step 1 mycelium that aforementioned one-level solid culture obtains, every 200ml liquid nutrient medium inoculation mycelium 3.5g, lucifuge 20 ~ 24 DEG C, 130 ~ 150 turns/min shaking table cultivates 48 hours, completes secondary expanding species liquid culture when there is small rice grain sample mycelium pellet;
3) Chinese caterpillar fungus solids manufacture is cultivated:
1. solids manufacture medium is prepared: medium component proportioning is: rice 1100g, distilled water 1300g, peptone 13g, glucose 19g, KH 2pO 42.5g, 7H 2oMgSO 41.2g, selenium methionine 0.6g, VB 12.5mg, recording pH value is 6.8; First dissolved by each composition distilled water except rice, then load in blake bottle by rice and nutrient solution by weight the ratio of 1:1.2, sealing, 95-100 DEG C is steamed 35 minutes, is cooled to room temperature stand-by;
2. inoculate, cultivate: aseptically inoculate with solid culture medium prepared by step 1 culture fluid that said second expanding species liquid culture obtains, every 100g rice inoculation 0.5ml culture fluid; Put into and cultivate 18 ~ 23 DEG C, darkroom lucifuge cultivation 8-10 days; Put into natural daylight or artificial lighting when media surface grows white hypha, 18 ~ 23 DEG C of ambient growth 40-55 days, when stroma is long neat, tip is expanded, sporulation time can gather.
Utilizing the method in Chinese Pharmacopoeia to record in the Cordceps militaris nurtured cordycepin content in every 100g is 210.5mg; Recording Se content is 0.31mg/kg, reaches the Se content level of wild Chinese caterpillar fungus.

Claims (6)

1. a high cordycepin selenium-rich cordyceps cultivating method, is characterized in that, comprises the steps:
1) Cordyceps Militaris one-level solid culture:
1. inclined-plane solid culture medium is prepared: medium component proportioning is: potato 100-140g, glucose 10-14g, beef extract 4-6g, KH 2pO 40.4-0.6g, MgSO 40.2-0.3g, selenium methionine 0.008-0.012g, agar 8-12g; First boiled by potato, mash, add all the other compositions aforementioned, heating is boiled and is allowed agar melt completely, and adding distil water is to 500ml, and stir dress inclined-plane processed, and sterilizing is chilled to room temperature and prepares inoculation;
2. inoculate, cultivate: the mycelium aseptically inoculating Chinese caterpillar fungus with inclined-plane solid culture medium prepared by step 1, lucifuge 24 DEG C cultivates 3-4 days, then cultivates 4-5 days at 20 DEG C, then 18 ~ 22 DEG C of natural lightings 24 hours, stand-by;
2) Cordyceps Militaris secondary expanding species liquid culture:
1. secondary liquid medium is prepared: with distilled water obtaining liq medium, each constituent content is: peptone 13-17g/L, glucose 18-22g/L, KH 2pO 40.8-1.2g/L, 7H 2oMgSO 40.4-0.6g/L, VB 18-12mg/L, selenium methionine 0.04-0.06g/L, sterilizing is stand-by;
2. inoculate, cultivate: aseptically inoculate with liquid nutrient medium prepared by step 1 mycelium that aforementioned one-level solid culture obtains, lucifuge 20 ~ 24 DEG C, shaking table is cultivated, and completes secondary expanding species liquid culture when there is small rice grain sample mycelium pellet;
3) Chinese caterpillar fungus solids manufacture is cultivated:
1. solids manufacture medium is prepared: medium component proportioning is: rice 800-1200g, distilled water 1100-1300g, peptone 10-14g, glucose 16-20g, KH 2pO 41.5-2.5g, 7H 2oMgSO 40.8-1.2g, selenium methionine 0.4-0.6g, VB 11.5-2.5mg; First each composition distilled water except rice is dissolved preparation and become nutrient solution, then rice and nutrient solution are loaded in proportion in blake bottle, sealing, steams to rice ripe, is cooled to room temperature stand-by;
2. inoculate, cultivate: aseptically inoculate with solid culture medium prepared by step 1 culture fluid that said second expanding species liquid culture obtains; Put into and cultivate the lucifuge cultivation of 18 ~ 23 DEG C, darkroom; Put into natural daylight or artificial lighting when media surface grows white hypha, 18 ~ 23 DEG C of ambient growth, when stroma is long neat, tip is expanded, sporulation time can gather.
2. high cordycepin selenium-rich cordyceps cultivating method according to claim 1, is characterized in that, in described step 2 Cordyceps Militaris secondary expanding species liquid culture, after inoculation, shaking table is cultivated is that 130 ~ 150 turns/min shaking table cultivates 48 hours.
3. high cordycepin selenium-rich cordyceps cultivating method according to claim 1, is characterized in that, in the Cordyceps Militaris secondary expanding species liquid culture step in described step 2, and the mycelium 3-3.5g that every 200ml liquid nutrient medium inoculation one-level solid culture obtains.
4. high cordycepin selenium-rich cordyceps cultivating method according to claim 1, is characterized in that, in described step 3, Chinese caterpillar fungus solids manufacture is cultivated, rice and nutrient solution are by weight in the ratio loading blake bottle of 1:1.2, sealing, 95-100 DEG C is steamed 30-40 minute, is cooled to room temperature stand-by.
5. high cordycepin selenium-rich cordyceps cultivating method according to claim 1, it is characterized in that, in described step 3, Chinese caterpillar fungus solids manufacture is cultivated, the culture fluid obtained by solid culture medium inoculation secondary expanding species liquid culture, and inoculum concentration is every 100g rice inoculation 0.5ml culture fluid.
6. high cordycepin selenium-rich cordyceps cultivating method according to claim 1, is characterized in that, in described step 3, Chinese caterpillar fungus solids manufacture is cultivated, and puts into and cultivate 18 ~ 23 DEG C, darkroom lucifuge cultivation 8-10 days after inoculation.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104871827A (en) * 2015-06-11 2015-09-02 孙建然 Artificially cultivated cordyceps sinensis and cultivation method thereof
CN106069177A (en) * 2016-06-12 2016-11-09 鲁东大学 The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content
CN106561264A (en) * 2016-11-02 2017-04-19 仪征康海农业发展有限公司 High-selenium high-cordycepin-content cordyceps militaris culturing method and culture medium thereof
CN106591150A (en) * 2016-12-16 2017-04-26 山西省肿瘤研究所 Selenium-rich isaria feline culture substance and culture method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020087194A (en) * 2001-05-14 2002-11-22 주식회사 엔에스케이텍 Solid cultivation method of cordyceps sinensis
CN101897275A (en) * 2010-08-13 2010-12-01 义乌市丹溪药用生物开发研究所 Selenium-rich cordyceps culturing method
CN102138436A (en) * 2010-12-28 2011-08-03 湖北圣峰药业有限公司 Method for culturing selenium-enriched Cordyceps militaris
CN102428834A (en) * 2011-09-20 2012-05-02 骆新春 Cultivation method of selenium-enriched Cordyceps militaris mother culture
CN103609329A (en) * 2013-11-05 2014-03-05 昆山市康乐虫草专业合作社 Cordyceps militaris culturing method capable of improving cordycepin content

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020087194A (en) * 2001-05-14 2002-11-22 주식회사 엔에스케이텍 Solid cultivation method of cordyceps sinensis
CN101897275A (en) * 2010-08-13 2010-12-01 义乌市丹溪药用生物开发研究所 Selenium-rich cordyceps culturing method
CN102138436A (en) * 2010-12-28 2011-08-03 湖北圣峰药业有限公司 Method for culturing selenium-enriched Cordyceps militaris
CN102428834A (en) * 2011-09-20 2012-05-02 骆新春 Cultivation method of selenium-enriched Cordyceps militaris mother culture
CN103609329A (en) * 2013-11-05 2014-03-05 昆山市康乐虫草专业合作社 Cordyceps militaris culturing method capable of improving cordycepin content

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104871827A (en) * 2015-06-11 2015-09-02 孙建然 Artificially cultivated cordyceps sinensis and cultivation method thereof
CN106069177A (en) * 2016-06-12 2016-11-09 鲁东大学 The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content
CN106561264A (en) * 2016-11-02 2017-04-19 仪征康海农业发展有限公司 High-selenium high-cordycepin-content cordyceps militaris culturing method and culture medium thereof
CN106561264B (en) * 2016-11-02 2019-06-04 江苏康能生物工程股份有限公司 A kind of cultural method and its culture medium of high selenium high cordycepin content Cordyceps militaris
CN106591150A (en) * 2016-12-16 2017-04-26 山西省肿瘤研究所 Selenium-rich isaria feline culture substance and culture method thereof

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