CN104388500A - Method for high density fermentation of spinosad - Google Patents
Method for high density fermentation of spinosad Download PDFInfo
- Publication number
- CN104388500A CN104388500A CN201410456028.6A CN201410456028A CN104388500A CN 104388500 A CN104388500 A CN 104388500A CN 201410456028 A CN201410456028 A CN 201410456028A CN 104388500 A CN104388500 A CN 104388500A
- Authority
- CN
- China
- Prior art keywords
- pleocidin
- vitamin
- dissolved oxygen
- fermentation
- seed culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for high density fermentation of spinosad. The invention provides a method for culturing eukaryotic microorganisms. The method includes strain mutagenesis and gene screening, medium improvement, precise control and improvement of fermentation process, and improvement of the extraction process. Accordingly, the fermentation titer of spinosad can reach over 6500mg/L, and the purity of spinosad can reach 98%.
Description
Technical field
The invention belongs to bioengineering field, relate to a kind of method of high density fermentation pleocidin
Background technology
Pleocidin is that a kind of novel green spectral biotic pesticide belong to microbial source biochemical pesticides, pleocidin has the security of biological pesticide and the low-hanging fruit of chemical pesticide concurrently, after spray medicine, the same day can take effect, the safe collection period of China and U.S.A Ministry of Agriculture registration is all one day, the production of the most applicable pollution-free vegetable has been widely used in Agricultural pests and zooparasite prevents and treats, and becomes biotic pesticide most with prospects in the world at present., because of its low toxicity, low residue, to natural enemies of insects' safety, natural decomposition is fast and obtain continuous three acquisitions U.S. " presidential green chemical Challenge Awards ".Pleocidin has many good qualities: day by day focusing on today of environmental protection and awareness of safety, and pleocidin, as a kind of green bio agricultural chemicals of high-quality, has huge development and application prospect.The main method adopting biological fermentation produced by current pleocidin, and use traditional spontaneons screening, the raising of the multiple method such as selection by mutation to pleocidin output is relatively limited.Pleocidin stings saccharopolyspora strain fermentative production by actinomycetes, and two glycosyls in pleocidin molecular structure, namely rhamnosyl and good fortune strangle osamine, and they have common precursor NDP-4-ketone-6 DDG.The synthesis step of this common precursor is the rate-limiting step of the biosynthetic pathway of pleocidin.And this NDP-4-ketone-6 DDG synthase gene and NDP-glucose sugar reductase gene synthesize shared a set of gene, not in pleocidin gene cluster with rhamnosyl in thorn saccharopolyspora strain cell walls.Dow AgroSciences passes through homologous probe, doubled the whole cosmid synthetic gene of this common precursor, on the basis of wild strain, the output of pleocidin has had significant raising, pleocidin has degraded completely, has broad safety limit to the even most of beneficial insect of Mammals, birds, fish.Pleocidin selects toxicity ratio higher than other common pesticides, larger than Avrmectin 300-400 times.
At present, restrict the subject matter that pleocidin realizes industrialization and wide popularization and application and be that the pleocidin in zymotechnique and fermented liquid is tired on the low side; Major cause: bacterial classification does not screen, spawn degeneration is serious; Fermention medium poor effect, sting thalline in saccharopolyspora strain fermenting process is thread growth simultaneously, and the biosynthesizing of pleocidin needs lipid acid as precursor, other materials such as grease, sugar and other materials that in fermentative production, normal interpolation is certain, because fermentation control technique is too simple, cause thalline fermented liquid very thickness, cause for hypoxgia, cannot suitability for industrialized production be met.
Summary of the invention
1, the shortening of fermentation time
2, adopt solvent or diluent formula, greatly reduce the viscosity of fermented liquid, add the dissolved oxygen effect of fermented liquid, thus reduce air device and motor power consumption, what make fermenting process can lower more than 20
3, the consumption of sugar is reduced
4, the content of COD in fermented liquid is reduced
Embodiment
Use example so that the present invention to be described below, it should be understood that example is for illustration of the present invention instead of limitation of the present invention.Scope of the present invention and core content are determined according to claims
Example one:
1, the bacterial classification of following embodiment selects pleocidin bacterial classification: the bacterial classification source of production and application is female bottle enriched liquid substratum, 3-5 days cultivated by fermented liquid, microscopy is without miscellaneous bacteria, thalli morphology is sturdy, without the situation of fracture, after three batches of checkings, diastatic activity is high, what spore quantity was many is engineering strain, adopts engineering strain to carry out follow-up fermentation.
2, the primary-seed medium sterilizing in 5L fermentor tank is also cooled to 31 DEG C, and uses sterile air pressurize, and then under flame protection, cultured pleocidin bacterial classification is inoculated into first class seed pot and cultivates, inoculum size controls within 3L.In first order seed culturing process, first order seed culture condition 30-34 DEG C, tank pressure 0.05-0.07Kg/cm2, air flow 1.2-1.8vvm, pH are between 6-7, cultivate 3-5 days.First 10 hours of seed culture, tank temperature control is at 30-31 DEG C, and 11 little cultivations up to first order seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, and start mechanical stirring, rotating speed controls within 80rpm, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 50%.
Unless otherwise indicated, the fermention medium used in this paper case study on implementation part comprises following composition, the wherein digital aimed concn pointed out: glucose 20g/L, analysis for soybean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L Sodium orthomolybdate 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2, the mixture of 3-the third 3 triol, consumption is 2ppm, and the pH value after medium liquid sterilizing is: 6.0-6.8
Secondary seed medium sterilizing in 30L fermentor tank is also cooled to 31 DEG C, and uses sterile air pressurize, primary seed solution is all moved into secondary seed tank and cultivates.In secondary seed culturing process, air flow 1.2-1.8vvm, pH are between 6-7.4, tank pressure 0.05-0.07Kg/cm2; 0-10 hour, tank temperature control is at 30-31 DEG C, and 11 little cultivations up to secondary seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, and start mechanical stirring, rotating speed controls within 80rpm, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 40%.
Unless otherwise indicated, the fermention medium used in this paper case study on implementation part comprises following composition, wherein the digital aimed concn pointed out, secondary seed medium composition is as follows: glucose 10g/L, starch 15g/L, analysis for soybean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L Sodium orthomolybdate 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2,3-the third 3 mixture of triol, consumption is 2ppm
The fermention medium used in this paper case study on implementation part comprises following composition, the wherein digital aimed concn pointed out, secondary seed medium composition is as follows: glucose 10g/L, starch 15g/L, analysis for soybean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L Sodium orthomolybdate 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2, the mixture of 3-the third 3 triol, consumption is 2ppm, and the pH value after medium liquid sterilizing is: 6.0-6.8
Three grades of seed culture medium sterilizings in 100L fermentor tank are also cooled to 31 DEG C, and use sterile air pressurize, primary seed solution are all moved into secondary seed tank and cultivate.In secondary seed culturing process, air flow 1.2-1.8vvm, pH are between 6.8-7.2, tank pressure 0.04-0.08Kg/cm2; 0-10 hour, tank temperature control is at 30-31 DEG C, and 11 little cultivations up to secondary seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, starts mechanical stirring, rotating speed controls according to dissolved oxygen level adjustment, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 40%.
Fermentation results: ferment by 216 hours, cell cell concentration is 43%, and pleocidin content is 7200ug/L.
Example two:
1, the bacterial classification of following embodiment selects pleocidin bacterial classification: the bacterial classification source of production and application is female bottle enriched liquid substratum, 3-5 days cultivated by fermented liquid, microscopy is without miscellaneous bacteria, thalli morphology is sturdy, without the situation of fracture, after three batches of checkings, diastatic activity is high, what spore quantity was many is engineering strain, adopts engineering strain to carry out follow-up fermentation.
2, the primary-seed medium sterilizing in 30L fermentor tank is also cooled to 31 DEG C, and uses sterile air pressurize, and then under flame protection, cultured pleocidin bacterial classification is inoculated into first class seed pot and cultivates, inoculum size controls within 3L.In first order seed culturing process, first order seed culture condition 30-34 DEG C, tank pressure 0.05-0.07Kg/cm2, air flow 1.2-1.8vvm, pH are between 6-7, cultivate 3-5 days.First 10 hours of seed culture, tank temperature control is at 30-31 DEG C, and 11 little cultivations up to first order seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, and start mechanical stirring, rotating speed controls within 80rpm, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 50%.
Unless otherwise indicated, the fermention medium used in this paper case study on implementation part comprises following composition, the wherein digital aimed concn pointed out: glucose 20g/L, bean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L Sodium orthomolybdate 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2, the mixture of 3-the third 3 triol, consumption is 2ppm, and the pH value after medium liquid sterilizing is: 6.0-6.8
Secondary seed medium sterilizing in 200L fermentor tank is also cooled to 31 DEG C, and uses sterile air pressurize, primary seed solution is all moved into secondary seed tank and cultivates.In secondary seed culturing process, air flow 1.2-1.8vvm, pH are between 6-7.4, tank pressure 0.05-0.07Kg/cm2; 0-10 hour, tank temperature control is at 30-31 DEG C, and 11 little cultivations up to secondary seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, and start mechanical stirring, rotating speed controls within 80rpm, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 40%.
Unless otherwise indicated, the fermention medium used in this paper case study on implementation part comprises following composition, wherein the digital aimed concn pointed out, secondary seed medium composition is as follows: glucose 10g/L, starch 15g/L, analysis for soybean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L Sodium orthomolybdate 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2,3-the third 3 mixture of triol, consumption is 2ppm
The fermention medium used in this paper case study on implementation part comprises following composition, the wherein digital aimed concn pointed out, secondary seed medium composition is as follows: glucose 10g/L, starch 15g/L, analysis for soybean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L Sodium orthomolybdate 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2, the mixture of 3-the third 3 triol, consumption is 2ppm, and the pH value after medium liquid sterilizing is: 6.0-6.8
Three grades of seed culture medium sterilizings in 2000L fermentor tank are also cooled to 31 DEG C, and use sterile air pressurize, primary seed solution are all moved into secondary seed tank and cultivate.In secondary seed culturing process, air flow 1.2-1.8vvm, pH are between 6.8-7.2, tank pressure 0.04-0.08Kg/cm2; 0-10 hour, tank temperature control is at 30-31 DEG C, and 11 little cultivations up to secondary seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, starts mechanical stirring, rotating speed controls according to dissolved oxygen level adjustment, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 40%.
Fermentation results: ferment by 216 hours, cell cell concentration is 43%, and pleocidin content is 6500ug/L.
Example three:
1, the bacterial classification of following embodiment selects pleocidin bacterial classification: the bacterial classification source of production and application is female bottle enriched liquid substratum, 3-5 days cultivated by fermented liquid, microscopy is without miscellaneous bacteria, thalli morphology is sturdy, without the situation of fracture, after three batches of checkings, diastatic activity is high, what spore quantity was many is engineering strain, adopts engineering strain to carry out follow-up fermentation.
2, the primary-seed medium sterilizing in 50L fermentor tank is also cooled to 31 DEG C, and uses sterile air pressurize, and then under flame protection, cultured pleocidin bacterial classification is inoculated into first class seed pot and cultivates, inoculum size controls within 3L.In first order seed culturing process, first order seed culture condition 30-34 DEG C, tank pressure 0.05-0.07Kg/cm2, air flow 1.2-1.8vvm, pH are between 6-7, cultivate 3-5 days.First 10 hours of seed culture, tank temperature control is at 30-31 DEG C, and 11 little cultivations up to first order seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, and start mechanical stirring, rotating speed controls within 80rpm, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 50%.
Unless otherwise indicated, the fermention medium used in this paper case study on implementation part comprises following composition, the wherein digital aimed concn pointed out: glucose 20g/L, bean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L Sodium orthomolybdate 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2, the mixture of 3-the third 3 triol, consumption is 2ppm, and the pH value after medium liquid sterilizing is: 6.0-6.8
Secondary seed medium sterilizing in 500L fermentor tank is also cooled to 31 DEG C, and uses sterile air pressurize, primary seed solution is all moved into secondary seed tank and cultivates.In secondary seed culturing process, air flow 1.2-1.8vvm, pH are between 6-7.4, tank pressure 0.05-0.07Kg/cm2; 0-10 hour, tank temperature control is at 30-31 DEG C, and 11 little cultivations up to secondary seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, and start mechanical stirring, rotating speed controls within 80rpm, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 40%.
Unless otherwise indicated, the fermention medium used in this paper case study on implementation part comprises following composition, wherein the digital aimed concn pointed out, secondary seed medium composition is as follows: glucose 10g/L, starch 15g/L, analysis for soybean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L molybdic acid receives 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2,3-the third 3 mixture of triol, consumption is 2ppm
The fermention medium used in this paper case study on implementation part comprises following composition, the wherein digital aimed concn pointed out, secondary seed medium composition is as follows: glucose 10g/L, starch 15g/L, analysis for soybean powder 10g/L, cottonseed meal 5g/L, yeast extract paste 6g/L, Sodium Glutamate 10g/L, potassium primary phosphate is 1.5g/L, ammonium sulfate 3g/L, calcium carbonate 2g/L, VITMAIN B1 6mg/L; Vitamin B6 7mg/L; Vitamin B12 0.15mg/L, manganous sulfate 3mg/L, zinc sulfate 3.5mg/L Sodium orthomolybdate 0.02mg/L, cobalt chloride 0.04mg/L, ferrous sulfate 10mg/L, defoamer: methyl oxirane and oxyethane and 1,2, the mixture of 3-the third 3 triol, consumption is 2ppm, and the pH value after medium liquid sterilizing is: 6.0-6.8
Three grades of seed culture medium sterilizings in 5000L fermentor tank are also cooled to 31 DEG C, and use sterile air pressurize, primary seed solution are all moved into secondary seed tank and cultivate.In secondary seed culturing process, air flow 1.2-1.8vvm, pH are between 6.8-7.2, tank pressure 0.04-0.08Kg/cm2:0-10 hour, and tank temperature control is at 30-31 DEG C, and 11 little cultivations up to secondary seed are terminated, and tank temperature control is at 32-33 DEG C; Alr mode: 0-10 hour, adopt gas automatic turning to replace mechanical stirring, 11 is little in first order seed culturing process, starts mechanical stirring, rotating speed controls according to dissolved oxygen level adjustment, and the dissolved oxygen level in whole one-level culturing process maintains more than at least 40%.
Fermentation results: ferment by 216 hours, cell cell concentration is 43%, and pleocidin content is 6900ug/L.
Claims (1)
1. the method for a high density fermentation pleocidin, with actinomycetes thorn saccharopolyspora strain (Saccharopolyspora spinosa) CGMCC for fermentation strain, by high cell density fermentation, improve the fermentation level of pleocidin, it is characterized in that, comprise the following steps:
1) seed culture: actinomycetes thorn saccharopolyspora strain (Saccharopolyspora spinosa) CGMCC of the actinomycetes be kept in glycerine pipe thorn saccharopolyspora strain (Saccharopolyspora spinosa) CGMCC or slant culture is inoculated in a kind of liquid seed culture medium, first order seed culture condition 30-34 DEG C, tank pressure 0.05-0.07Kg/cm2, air flow 1.2-1.8vvm, pH is between 6-7, cultivates 3-5 days.Secondary seed culture condition 28-30 DEG C, the dissolved oxygen level in fermented liquid maintains more than at least 50%, tank pressure 0.04-0.06Kg/cm2, air flow 1.1-1.5vvm, pH is between 6-7.5, and cultivate 2-3 days, the dissolved oxygen level in fermented liquid maintains more than at least 50%.
Wherein a secondary liquid seed culture based formulas is: glucose 20-40g/L, analysis for soybean powder 10-15g/L, yeast extract paste 6-10g/L, Sodium Glutamate 5g/L-10g/L, potassium primary phosphate is 1.5g/L-3g/L, ammonium sulfate 3-5g/L, calcium carbonate 2g/L-4g/L, VITMAIN B1 2mg/L-8mg/L; Vitamin B6 2mg/L-8mg/L; Vitamin B12 0.1-0.4mg/L.
2) cultivate in secondary liquid seed culture transferring to fermention medium, culture condition 28-30 DEG C, dissolved oxygen level in fermented liquid maintains more than at least 50%, tank pressure 0.04-0.06Kg/cm2, air flow 1.1-1.5vvm, pH is between 6.8-7.2, and cultivate 2-3 days, the dissolved oxygen level in fermented liquid maintains more than at least 30%.
3) stage and object product induction period is improved according to comprising biomass high-density described in step 2, wherein the high density fermentation stage comprises the described carbon source of interpolation and described limiting nutrient source, this object product induction period comprises described carbon source, do not add or seldom add described limiting nutrient source and produce nutrient limiting condition to induce, this condition can induce generation pleocidin.
4) described in step 2, fermentative medium formula:
A () adds carbon source and limiting nutrient source in fermention medium, and be that dissolved oxygen level in fermented liquid maintains more than at least 50%, and pH remains between 6-7.5.
B () carbon source is glucose, maltose, dextrin, starch, isopropylcarbinol, propyl carbinol or its combination, content is 30g/L-60g/L.
C () nitrogenous source is yeast extract paste, yeast powder, analysis for soybean powder, Sodium Glutamate, cottonseed meal, casein peptone, content is 20g/-30g/L.
D () vegetables oil, be rapeseed oil, soybean oil, Oleum Gossypii semen, Trisun Oil R 80, content is 5g/L-10g/L, and vegetables oil is the vegetables oil of modification, adds lipase and carries out modification.
((e) magnesium sulfate 2g/L-5g/L, potassium primary phosphate 1.5g/L-4g/L, sodium bicarbonate 0.1g/L-0.6g/L, Calcium hydrogen carbonate 0.1g/L-0.6g/L, sodium-chlor 2g/L-6g/L.
(f) vitamins nutrition source: VITMAIN B1 2mg/L-8mg/L; Vitamin B6 2mg/L-8mg/L; Vitamin B12 0.1-0.4mg/L.
(g) manganous sulfate 2mg/L-5mg/L, zinc sulfate 3mg/L-7mg/L Sodium orthomolybdate 0.02mg/L-0.06mg/L, cobalt chloride 0.02mg/L-0.06mg/L, ferrous sulfate 5-15mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410456028.6A CN104388500A (en) | 2014-09-10 | 2014-09-10 | Method for high density fermentation of spinosad |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410456028.6A CN104388500A (en) | 2014-09-10 | 2014-09-10 | Method for high density fermentation of spinosad |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104388500A true CN104388500A (en) | 2015-03-04 |
Family
ID=52606457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410456028.6A Pending CN104388500A (en) | 2014-09-10 | 2014-09-10 | Method for high density fermentation of spinosad |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104388500A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365813A (en) * | 2017-07-03 | 2017-11-21 | 齐鲁制药（内蒙古）有限公司 | A kind of pleocidin fermentation process for improving cytoactive |
| CN107815479A (en) * | 2016-09-12 | 2018-03-20 | 牡丹江佰佳信生物科技有限公司 | A kind of fermentation process for improving multiple killing teichomycin yield |
| CN109628529A (en) * | 2019-01-11 | 2019-04-16 | 内蒙古拜克生物有限公司 | A kind of fermentation process improving pleocidin yield |
| CN111484959A (en) * | 2020-06-05 | 2020-08-04 | 宁夏泰益欣生物科技有限公司 | Culture medium and culture method for producing pleocidin by utilizing saccharopolyspora spinosa fermentation |
| CN113444659A (en) * | 2021-06-17 | 2021-09-28 | 武汉大学 | Saccharopolyspora spinosa for high yield of spinosad |
| CN114015604A (en) * | 2021-11-10 | 2022-02-08 | 河北兴柏农业科技有限公司 | Fermentation medium and method for producing pleocidin through fermentation |
-
2014
- 2014-09-10 CN CN201410456028.6A patent/CN104388500A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107815479A (en) * | 2016-09-12 | 2018-03-20 | 牡丹江佰佳信生物科技有限公司 | A kind of fermentation process for improving multiple killing teichomycin yield |
| CN107365813A (en) * | 2017-07-03 | 2017-11-21 | 齐鲁制药（内蒙古）有限公司 | A kind of pleocidin fermentation process for improving cytoactive |
| CN107365813B (en) * | 2017-07-03 | 2021-01-05 | 齐鲁制药（内蒙古）有限公司 | Spinosad fermentation method for improving cell activity |
| CN109628529A (en) * | 2019-01-11 | 2019-04-16 | 内蒙古拜克生物有限公司 | A kind of fermentation process improving pleocidin yield |
| CN109628529B (en) * | 2019-01-11 | 2020-01-17 | 内蒙古拜克生物有限公司 | Fermentation method for increasing yield of spinosad |
| CN111484959A (en) * | 2020-06-05 | 2020-08-04 | 宁夏泰益欣生物科技有限公司 | Culture medium and culture method for producing pleocidin by utilizing saccharopolyspora spinosa fermentation |
| CN113444659A (en) * | 2021-06-17 | 2021-09-28 | 武汉大学 | Saccharopolyspora spinosa for high yield of spinosad |
| CN113444659B (en) * | 2021-06-17 | 2022-10-04 | 武汉大学 | Saccharopolyspora spinosa for high yield of spinosad |
| CN114015604A (en) * | 2021-11-10 | 2022-02-08 | 河北兴柏农业科技有限公司 | Fermentation medium and method for producing pleocidin through fermentation |
| CN114015604B (en) * | 2021-11-10 | 2023-12-15 | 河北兴柏农业科技股份有限公司 | Fermentation medium and method for producing spinosad by fermentation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104388500A (en) | Method for high density fermentation of spinosad | |
| CN102174449B (en) | Method for producing high-yield gamma-propalanine and application thereof | |
| CN102160642B (en) | Method for preparing Cordyceps rice food | |
| CN102978122B (en) | Gibberella and method for fermentation production of gibberellin GA4+7 | |
| CN101239847B (en) | Liquid composite microbial fertilizer and its preparation method | |
| CN102630490B (en) | Artificial cultivation method for cordyceps militaris mycelium for increasing cordycepin content | |
| CN103194410B (en) | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same | |
| CN102757896A (en) | Efficient microbial soil remediation agent | |
| US20240102058A1 (en) | Caproate-producing bacterium with multiple substrate utilization capabilities and its applications | |
| CN105087680A (en) | Lactobacillus fermentation culture medium and process for producing lactic acid at high yield | |
| CN103820350A (en) | Method for producing bacillus amyloliquefaciens microbial fertilizer through food waste recycling | |
| CN105039194A (en) | Yeast fused strain mixed bacterial agent and method of fermenting organic waste water therewith to prepare liquid fertilizer | |
| CN105274178B (en) | A kind of composite bacteria agent that lignite ex situ is produced the method for methane coproduction humic acid and wherein applied | |
| CN104789619A (en) | Preparation method for MEL (Mannosylerythritol lipids) | |
| CN104152378A (en) | Compound microbial agent for anaerobic digestion treatment of sludge and production method of compound microbial agent | |
| CN104450529A (en) | Schizochytrium limacinum strain as well as mutagenesis method and application thereof | |
| CN104860756A (en) | Cordyceps militaris fermentation liquid culture medium, preparation method thereof and application thereof | |
| CN109234346A (en) | A kind of fermentation process producing vitamin B2 | |
| CN102381896B (en) | Crab-flavor mushroom liquid strain medium formula and preparation method thereof | |
| CN109429911A (en) | A kind of fermentation cordyceps sinensis culture solution and preparation method thereof prepared with jerusalem artichoke | |
| CN103451244B (en) | A kind of faecium is preparing the application in Pfansteihl | |
| CN103276019A (en) | Method for promoting lycopene synthesis in blakeslea trispora | |
| CN104016733B (en) | Yeast wastewater resource produces multifunctional biological fertilizer | |
| CN102533570A (en) | Aspergillus niger, application of Aspergillus niger and method for preparing citric acid by fermentation | |
| CN102676618B (en) | Method for producing abamectin through fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150304 |
|
| WD01 | Invention patent application deemed withdrawn after publication |