CN104388341B - A kind of colloid bacillus cereus bacterial strain and its application in marine alga is degraded - Google Patents
A kind of colloid bacillus cereus bacterial strain and its application in marine alga is degraded Download PDFInfo
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Abstract
The present invention relates to a kind of colloid bacillus cereus bacterial strain, Classification And Nomenclature is colloid bacillus cereus (Bacillus mucilaginosus), China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on November 15th, 2013, deposit number is CGMCC No.8478.The invention further relates to application of the colloid bacillus cereus in marine alga is degraded.Colloid bacillus cereus bacterial strain of the present invention has stronger marine alga degradation capability, and using the strains for degrading marine alga, the content >=15g/l of alginic acid, significantly improves compared with existing biodegradation technique in gained degradation solution.Additionally, the bacterial strain also ability with strong degraded cellulose, protein and algin, ties up element enzyme activity >=48.5U/ml, protease activity >=4.5U/ml, algin cracking enzyme activity >=0.855U/ml in zymotic fluid.
Description
Technical field
The present invention relates to a kind of colloid bacillus cereus bacterial strain and its application in marine alga is degraded, belong to biotechnology (micro-
It is biological) field.
Background technology
Marine alga (Alga) is submarine algae, is the cryptogam of plant kingdom.They are generally considered to be simple
Plant, be mainly characterized by：Without vascular tissue, there is no the differentiating phenomenon of real root, stem, leaf；Do not bloom, without fruit and kind
Son；Protective tissue of the reproductive organs without specialization, often directly produces spore or gamete by single cell；And the formation without embryo.
Marine alga belongs to rudimentary plant, and species is various.According to the difference of contained pigment in marine alga, green alga (such as sea lettuce), brown alga can be classified as
(such as sea-tangle, sargassum, marine alga, bulk kelp), blue-green algae (such as Shu Maozao), xanthophyta (such as halosphaera), chrysophyceae (such as calcium plate algae).
In marine alga in addition to containing substantial amounts of non-nitrogen containing organic matter and a number of amino acid, protein and trace element,
Also contain the mineral element such as algal polysaccharides specific to marine organisms, alginic acid and the rare zinc of terrestrial plant, bromine, iodine.
Alginic acid is a kind of agriculture increasing agent of high-quality, and it is, with marine alga as raw material, to extract obtained with degradation technique.Sea
The biodegrading process of algae generally can be divided into chemical hydrolysis, physics extraction method and biological fermentation process.Most factories domestic and international at present
Family is main using chemical hydrolysis degraded marine alga, is that highly basic high temperature can be destroyed in marine alga using the maximum inferior position of chemical hydrolysis
The activity of source material.The principle of biological fermentation process be using microorganism produced in the metabolic process with marine alga etc. as nutrient it is many
Enzyme is planted, the macromolecular substances for constituting marine alga small molecule, water miscible material is degraded into, because fermentation process does not have chemical method
Highly basic and high temperature, also high pressure and low temperature without Physical, therefore the bioactivity in marine alga is intactly remained to greatest extent
Material and nutriment.
A kind of production method of functional marine alga fertilizer is disclosed in Chinese invention patent CN98101040.The method is used
Marine organisms brown alga is primary raw material, and with potassium hydroxide as digestive pharmaceutical, digestion temperature is 70 DEG C~100 DEG C, can make institute in brown alga
Nutriment, trace element and the spontaneous growth Auto-regulator for containing, as beneficial to the soluble status being absorbed by plants.The invention
The activity of marine alga endogenous substance can be destroyed as digestive pharmaceutical with potassium hydroxide, the using effect of alga fertilizer is reduced.
A kind of feed for pet additive containing marine natural product is disclosed in Chinese invention patent CN103689266.
A kind of feed for pet additive containing algal polysaccharides, cecropin, chitin and seawood meal of the disclosure of the invention, the additive can
Increase the resistance of pet animals, reduce the generation of intestines problem.Additive in the invention is mixture, no to protrude certain
The effect of composition, and cost is higher.
Chinese invention patent CN103039696 discloses a kind of preparation method of fermentation of seaweed biological feedstuff：Using unwrapping wire
Bacterium, saccharomycete, bacillus subtilis and lactic acid bacteria prepare composite bacteria agent, and marine alga is fermented.The shortcoming of the technology is technique
Complexity is, it is necessary to multi-cultur es are compounded.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of colloid bacillus cereus bacterial strain, using screening
A kind of colloid bacillus cereus strains for degrading marine alga, the content >=15g/l of alginic acid in gained degradation solution, with existing biodegradation
Technology is compared and significantly improved, in addition, other nutritional ingredients such as Tang oligosaccharide >=5g/l, reduced sugar >=2g/l, amino acid >=1g/
L, marine alga residue≤1% there has also been further improvement.
To achieve the above object, one of technical scheme, is to provide a kind of colloid bacillus cereus bacterial strain, the bacterium
The Classification And Nomenclature of strain is colloid bacillus cereus (Bacillus mucilaginosus), micro- in China on November 15th, 2013
Biological inoculum preservation administration committee common micro-organisms center preservation, deposit number is CGMCC No.8478.
Preferably, the bacterium colony size of the colloid bacillus cereus bacterial strain is 10-18mm, and water white transparency is circular, and edge is whole
Together, the smooth moistening in surface, basis of microscopic observation thalline is shaft-like, raw in gemma, there is pod membrane, and Gram-reaction is negative.
The two of technical scheme, are to provide a kind of application of colloid bacillus cereus bacterial strain in marine alga of degrading.
Preferably, the marine alga is any one in yellow tang, sargassum or sea-tangle.
Preferably, the content >=15g/l of alginic acid in marine alga degradation solution obtained by after the marine alga is degraded.
Preferably, Tang oligosaccharide >=5g/l, reduced sugar >=2g/l, ammonia in marine alga degradation solution obtained by after the marine alga is degraded
Base acid >=1g/l, marine alga residue≤1%.
Further, the culture medium of the degraded marine alga is marine alga 50-100 weight portions, peptone 6-10 weight portions, chlorine
Change sodium 1-2 weight portions, KH2PO40.5-1 weight portions, MgSO40.1-0.5 weight portions, add water to 1000 weight portions, regulation pH to
7-8。
The marine alga degradation solution can be used for animal feeding as a kind of feed addictive.
Beneficial effects of the present invention：
(1) colloid bacillus cereus bacterial strain of the present invention has stronger marine alga degradation capability, using strains for degrading sea
Algae, the content >=15g/L of alginic acid, significantly improves compared with existing biodegradation technique in gained degradation solution；
(2) other the nutritional ingredient Tang oligosaccharide >=5g/l after marine alga degraded, reduced sugar >=2g/l, amino acid >=1g/
L, marine alga residue≤1% also contains the bioactivators such as gibberellin, abscisic acid, ethene, glycine betaine, polyamines and zinc, bromine, iodine
Deng mineral element；
(3) separation screening of the present invention obtains one plant of colloid bacillus cereus, the bacterial strain have strong degraded cellulose, protein and
The ability of algin, dimension element enzyme activity >=48.5U/ml in zymotic fluid, protease activity >=4.5U/ml, algin cracking enzyme activity >=
0.855U/ml；
(4) marine alga degradation solution is added in animal feed, the trophic structure of feed can be improved, improve the utilization of feed
Rate, adjusts the organism metabolism of cultivated animals, enhance immunity and premunition, promotes growth.
Specific embodiment
Technical scheme is described in further detail with reference to specific embodiment.
(1) separation screening of colloid bacillus cereus
In July, 2013 samples 10 parts altogether from the green house of vegetables of Shandong Jiangnan, 10g samples is taken respectively and is suspended in 100ml lifes
In reason salt solution, 180rpm vibrates 30min.Take 100 μ l sample suspension even spread flat boards respectively, in the embodiment of the present invention with
Yellow tang homogenate culture medium is sterile nutrient solution, is calculated with gram per liter, and its composition is yellow tang 10.0, agar 15.0, water
1000ml, 7.5,121 DEG C of sterilizing 20min of pH value, cultivation temperature is 32 DEG C, and incubation time is 48 hours.It is inoculated into method of scoring
On new yellow tang flat board, pure bacterium is obtained by repeated multiple times line, 18 plants of pure bacterium are filtered out altogether.
Obtained strains picking single bacterium colony carries out shaking flask secondary screening, and Shake flask medium is homogenized culture medium from liquid yellow tang,
180rpm, cultivates 64h by 32 DEG C, and the speed of growth and bacterium number are compared, and bacterial strain J-2 grows surely in 1% yellow tang culture medium
Determine and bacterium number is higher, in 1% yellow tang fluid nutrient medium, after 40h, basis of microscopic observation yellow tang cell, cell membrane is degraded.
(2) taxonomic identification of colloid bacillus cereus
Bacterial strain J-2 morphological features：Bacterium colony size 10-18mm, water white transparency is circular, neat in edge, the smooth moistening in surface,
Basis of microscopic observation thalline is shaft-like, raw in gemma, there is pod membrane, and Gram-reaction is negative.
Bacterial strain J-2 physio-biochemical characteristics：It is shown in Table 1.
Molecular genetics taxonomic identification：The DNA of bacterial strain J-2 is extracted, with the DNA as template, 16S rDNA universal primers are
Primer, expands to 16S rDNA complete sequences, and PCR primer carries out 1% agarose gel electrophoresis, detects expanding effect.After cloning
Sample be sequenced.Sequence in gained sequence and GenBank databases is carried out into Blast analyses and comparison, and chooses similitude
Bacterial strain higher, Neighbor Joining method constructing system chadograms are taken using the softwares of Mega 4.0.Result shows, bacterial strain
J-2 is nearest with colloid bacillus cereus (Bacillus mucilaginosus) affiliation.Morphology in combination with bacterial strain is special
Seek peace physio-biochemical characteristics, be classified as colloid bacillus cereus.
Table 1
| Physiological and biochemical property | Bacterial strain J-2 | Colloid bacillus cereus |
| Catalase determination | + | + |
| Casein is hydrolyzed | + | + |
| Gelatin hydrolysis | - | - |
| Tyrosine hydrolysis | - | - |
| Phenylalanine deamination | - | - |
| Lecithinase is determined | - | - |
| Starch Hydrolysis | + | + |
| Grown in the culture medium containing 5%NaCl | - | - |
| V.P is tested | - | - |
| Indole test | - | - |
| Sugared tunning mannitol | +- | +- |
| Xylose | +- | +- |
| Glucose | +- | +- |
| L-arabinose | +- | +- |
+：It is positive；-：It is negative；+-：Produce acid not aerogenesis
The bacterial strain J-2 that will be screened carries out culture presevation, preservation time：On November 15th, 2013；Depositary institution：China is micro-
The common micro-organisms center preservation of biological inoculum preservation administration committee；Preservation place：Datun Road, Chaoyang District, Beijing City Chinese science
Institute of microbiology of institute；Deposit number：CGMCC No.8478.
(3) colloid bacillus cereus optimization culture conditions and Enzyme assay
Optimization fermentation medium, by adjusting carbon source, nitrogen source, marine alga addition, initial pH, inoculum concentration, liquid amount and hair
Ferment temperature optimizes condition of enzyme production.
Fermentative medium formula after optimization is：Marine alga 50-100 weight portions, peptone 6-10 weight portions, sodium chloride 1-2
Weight portion, KH2PO40.5-1 weight portions, MgSO40.1-0.5 weight portions, adjust pH to 7-8.
Enzyme activity after fermentation：The plain enzyme activity of dimension is 48.5U/mL, and protease activity is 4.5U/mL, and algin cracking enzyme activity is
0.853U/mL。
(4) technical process of colloid bacillus cereus degraded marine alga
(1) marine alga immersion
Marine alga is soaked 2-15 hours；
(2) physics is crushed
Soaked marine alga is smashed into grinding；
(3) preparation of fermentation medium
Take step (2) gained marine alga 50-100 weight portions, peptone 6-10 weight portions, sodium chloride 1-2 weight portions, KH2PO4
0.5-1 weight portions, MgSO40.1-0.5 weight portions, add water to 1000 weight portions, and pH is to 7-8 for regulation, obtains final product required fermented and cultured
Base；
(4) it is inoculated with and ferments
Colloid bacillus cereus (preserving number is CGMCC No.8478) are inoculated into step (3) gained culture medium, fermentation,
Obtain final product marine alga degradation solution.
Embodiment 1
(1) yellow tang immersion
50 kilograms of yellow tangs are taken, according to yellow tang: water=1: the ratio of 10 (weight ratios) adds water 500kg, is soaked 10 hours；
(2) physics is crushed
Yellow tang is smashed into grinding 30min with beating crusher；
(3) preparation of fermentation medium
Take step (2) gained yellow tang homogenate 80kg, addition 6kg peptones (0.6%, W/W), 1.2kg sodium chloride
(0.12%, W/W), 0.7kgKH2PO4(0.07%, W/W), 0.2kgMgSO4(0.02%, W/W), adds water to 1000L, using ammonia
Water adjusts medium pH to 7.0；
(4) colloid bacillus cereus J-2 seed activations
Bacillusmusilaginosiengineering J-2 (preserving number is CGMCC No.8478) is transferred in fresh tube from the inclined-plane for preserving
Inclined-plane, in 32 DEG C of constant incubator culture 24h.Cultured inclined-plane is taken, a full ring is chosen with oese, be inoculated in and fill 50mL
In the 250mL triangular flasks of aseptic seed culture medium, 32 DEG C of shaking table, 180r/min shaken cultivations 20h.Corresponding seed quality criteria：OD600
Value reaches 0.5 (5 times of dilution), and bacterial content reaches 8.0 × 108CFU, microscopy thalline is full, and gemma is just initially formed.Inoculum concentration
0.1%, it is inoculated with using flame inoculation method；
(5) ferment
Fermentation parameter control is as follows：Fermentation temperature：32℃；Zymotic fluid pH：7.0；Speed of agitator：0-4h, 120rpm；4-20h,
150rpm；20-24h, 180rpm.Throughput：0-4h, 1:0.5；4-10h, 1:0.8；10-20h, 1:1.2；20-24h, 1:0.5；
Tank pressure：0.02~0.03MPa.
Malleation in tank body is remained in fermentation process, and according to how much regulation tank pressures of foam in fermentation tank, foam
When excessive, can suitably increase tank pressure；Froth breaking：According to produce foam number, stream plus by way of add bubble enemy and (gone out
Bacterium), or the bubble enemy that 0.05~0.1% is directly added into culture medium process for preparation.The number of foam and throughput size, stir
The factor such as mix rotating speed height, broth viscosity, thalline content, defoamer be how many is relevant, can flexibly control during the fermentation；
Sampled every 2 hours, microscopy thalli morphology, OD600, the parameter such as sporulation ratio and total reducing sugar, total nitrogen.Fermentation
Time reaches 24 hours or so, and the gemma rate in the visual field stops tank when reaching 90%.Thalline content is up to 2.0 × 109CFU/ml, stops
Open cold water after tank to be lowered the temperature, temperature is typically reduced to 20 DEG C or so, obtains final product marine alga degradation solution.
Embodiment 2
(1) sargassum immersion
70 kilograms of sargassums are taken, according to sargassum: water=1: the ratio of 15 (weight ratios) adds water 1050kg, immersion 12 is small
When；
(2) physics is crushed
Sargassum is smashed into grinding 30min with beating crusher；
(3) preparation of fermentation medium
Take step (2) gained sargassum homogenate 90kg, addition 7kg peptones (0.7%, W/W), 1.5kg sodium chloride
(0.15%, W/W), 0.6kgKH2PO4(0.06%, W/W), 0.4kgMgSO4(0.04%, W/W), adds water to 1000L, using ammonia
Water adjusts medium pH to 7.5；
(4) colloid bacillus cereus J-2 seed activations
Bacillusmusilaginosiengineering J-2 (preserving number is CGMCC No.8478) is transferred in fresh tube from the inclined-plane for preserving
Inclined-plane, in 30 DEG C of constant incubator culture 24h.Cultured inclined-plane is taken, a full ring is chosen with oese, be inoculated in and fill 50ml
In the 250ml triangular flasks of aseptic seed culture medium, 30 DEG C of shaking table, 180r/min shaken cultivations 20h.Corresponding seed quality criteria：OD600
Value reaches 0.5 (5 times of dilution), and bacterial content reaches 8.0 × 108CFU, microscopy thalline is full, and gemma is just initially formed.Inoculum concentration
0.1%, it is inoculated with using flame inoculation method；
(5) ferment
Fermentation parameter control is as follows：Fermentation temperature：30℃；Zymotic fluid pH：7.5；Speed of agitator：0-4h, 120rpm；4-20h,
150rpm；20-24h, 180rpm.Throughput：0-4h, 1:0.5；4-10h, 1:0.8；10-20h, 1:1.2；20-24h, 1:0.5；
Tank pressure：0.02~0.03MPa.
The other the same as in Example 1
Embodiment 3
(1) kelp soaking
30 kilograms of sea-tangles are taken, according to sea-tangle: water=1: the ratio of 20 (weight ratios) adds water 600kg, is soaked 15 hours；
(2) physics is crushed
Sea-tangle is smashed into grinding 30min with fiberizer；
(3) preparation of fermentation medium
Take step (2) gained sea-tangle homogenate 85kg, addition 8kg peptones (0.8%, W/W), 1.4kg sodium chloride
(0.14%, W/W), 0.8kgKH2PO4(0.08%, W/W), 0.3kgMgSO4(0.03%, W/W), adds water to 1000L, using ammonia
Water adjusts medium pH to 7.8；
(4) colloid bacillus cereus J-2 seed activations
Bacillusmusilaginosiengineering J-2 (preserving number is CGMCC No.8478) is transferred in fresh tube from the inclined-plane for preserving
Inclined-plane, in 31 DEG C of constant incubator culture 24h.Cultured inclined-plane is taken, a full ring is chosen with oese, be inoculated in and fill 50ml
In the 250ml triangular flasks of aseptic seed culture medium, 31 DEG C of shaking table, 180r/min shaken cultivations 20h.Corresponding seed quality criteria：OD600
Value reaches 0.5 (5 times of dilution), and bacterial content reaches 8.0 × 108CFU, microscopy thalline is full, and gemma is just initially formed.Inoculum concentration
0.5%, it is inoculated with using flame inoculation method；
(5) ferment
Fermentation parameter control is as follows：Fermentation temperature：31℃；Zymotic fluid pH：7.8；Speed of agitator：0-4h, 120rpm；4-20h,
150rpm；20-24h, 180rpm.Throughput：0-4h, 1:0.5；4-10h, 1:0.8；10-20h, 1:1.2；20-24h, 1:0.5；
Tank pressure：0.02~0.03MPa.
The other the same as in Example 1.
Example 1-3 gained marine alga degradation solutions, analyze Tang oligosaccharide therein, alginic acid, reduced sugar, amino acid and
The content of marine alga residue, and cellulase activity, protease activity and the algin cracking enzyme activity of marine alga degradation solution are have detected, as a result see
Table 2.
Table 2
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Tang oligosaccharide, g/l | 6.2 | 5.8 | 7.1 |
| Alginic acid, g/l | 18.5 | 17.9 | 19.4 |
| Reduced sugar, g/l | 2.3 | 2.8 | 2.5 |
| Amino acid, g/l | 1.6 | 1.7 | 1.1 |
| Marine alga residue, % | 0.94 | 0.91 | 0.87 |
| Cellulase activity, U/ml | 49.8 | 51.2 | 50.9 |
| Protease activity, U/ml | 5.0 | 5.6 | 4.8 |
| Algin cracks enzyme activity, U/ml | 0.866 | 0.859 | 0.857 |
Application Example 1
The gained marine alga degradation solution of Example 1 is used for animal feeding：
In plant of Jiaonan City of Shandong Province, the yellow plumage Broiler chicks for choosing 150 1 ages in days are equally divided into tri- groups, every group of A, B, C
50, its raising situation is respectively：
A groups feed basal feed；
B groups feed the basal feed of 3% seawood meal of addition；
C groups feed the basal feed of 3% marine alga degradation solution of addition.
After raising 30 days, result is observed, the results are shown in Table 3.
Table 3
| Packet | Body weight (g) | Average daily feed intake (g/d) | Average weight increasing a day (g/d) | Feed-weight ratio | The death rate |
| A | 171 | 13.61 | 4.71 | 2.89 | 8% |
| B | 183 | 14.39 | 5.24 | 2.75 | 6% |
| C | 188 | 14.95 | 5.67 | 2.64 | 2% |
Claims (2)
1. a kind of colloid bacillus cereus bacterial strain, it is characterised in that：Its Classification And Nomenclature is colloid bacillus cereus (Bacillus
Mucilaginosus), on November 15th, 2013 in China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation, deposit number is CGMCC No.8478.
2. application of the bacterial strain described in a kind of claim 1 in marine alga of degrading, the marine alga is yellow tang, sargassum or sea-tangle
In any one.
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| CN104744162B (en) * | 2015-03-20 | 2018-01-26 | 济南航晨生物科技有限公司 | The method that marine alga intermediate is extracted by microbial degradation marine alga |
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| CN114317379A (en) * | 2022-01-28 | 2022-04-12 | 重庆融极浩瀚生物技术有限公司 | Complex microbial inoculant for producing high-activity cellulase as well as preparation method and application thereof |
| CN115553170A (en) * | 2022-09-15 | 2023-01-03 | 深圳中绿环境集团有限公司 | Method for constructing hardened seawall vegetation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816718A (en) * | 2012-08-08 | 2012-12-12 | 上海交通大学 | Bacillus and application thereof |
| CN103013851A (en) * | 2012-10-08 | 2013-04-03 | 上海交通大学 | Complex microbial inoculant and applications thereof |
| CN103710281A (en) * | 2013-11-12 | 2014-04-09 | 青岛明月蓝海生物科技有限公司 | Bacillus mucilaginosus strain and application thereof in seaweed biofertilizer |
-
2014
- 2014-10-29 CN CN201410593441.7A patent/CN104388341B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816718A (en) * | 2012-08-08 | 2012-12-12 | 上海交通大学 | Bacillus and application thereof |
| CN103013851A (en) * | 2012-10-08 | 2013-04-03 | 上海交通大学 | Complex microbial inoculant and applications thereof |
| CN103710281A (en) * | 2013-11-12 | 2014-04-09 | 青岛明月蓝海生物科技有限公司 | Bacillus mucilaginosus strain and application thereof in seaweed biofertilizer |
Non-Patent Citations (4)
| Title |
|---|
| Distribution of Alginate Lyase Activity among Strains of Bacillus circulans;JEFFREY B.HANSEN等;《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》;19850430;第49卷(第4期);1019-1021 * |
| Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis;TOMOHIRO HISANO等;《JOURNAL OF FERMENTATION AND BIOENGINEERING》;19941231;第78卷(第1期);79-83 * |
| 一株泡叶藻降解菌的筛选及其发酵条件优化;刘露 等;《山东农业大学学报（自然科学版）》;20141015;第45卷(第4期);515-521 * |
| 一株胶质芽胞杆菌YA-07的鉴定及固体发酵研究;田稼 等;《中国农业科技导报》;20141015;第16卷(第5期);67-77 * |
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