CN104388093B - A kind of compound biological soil improver and preparation method thereof - Google Patents
A kind of compound biological soil improver and preparation method thereof Download PDFInfo
- Publication number
- CN104388093B CN104388093B CN201410581434.5A CN201410581434A CN104388093B CN 104388093 B CN104388093 B CN 104388093B CN 201410581434 A CN201410581434 A CN 201410581434A CN 104388093 B CN104388093 B CN 104388093B
- Authority
- CN
- China
- Prior art keywords
- compound biological
- bacterium
- soil
- soil improver
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention discloses a kind of compound biological soil improver, it is using compound biological bacterium as core, and the compound biological bacterium is the combination of Paenibacillus polymyxa and bacillus subtilis；The invention also discloses the preparation method of the compound biological soil improver：Soil conditioner is subjected to deep liquid High Density Cultivation with biological bacteria respectively, zymotic fluid is obtained, bacterium mud is collected after centrifugal treating, protective agent is added and freeze-dried powder is made；Using compound biological bacterium as core, powdery organic fertilizer and perlite, obtain compound biological soil improver needed for mixing.The compound biological soil improver of the present invention, its number of viable is high, can preserve for a long time at normal temperatures, can carry out being used in mixed way with other organic and inorganic soil conditioners unaffected, and preventing and treating soil-borne disease effect is obvious.
Description
This application is the divisional application of following patent application：Application No.：200810208210.4, the applying date is 2008
December 25, entitled " a kind of compound biological soil improver and preparation method thereof ".
Technical field
The present invention relates to a kind of soil conditioner, more particularly to a kind of biological composite soil modifier；In addition, the present invention is also
It is related to the preparation method of the biological composite soil modifier.
Background technology
China is populous nation, a large agricultural country, and the population in the Liao Zhan worlds 22% is supported with the soil for accounting for the world 7%,
Food problem is the major issue for being related to national economy forever.Existing 2,000,000,000 mu of the arable land of China, needs 1.5 hundred million tons of chemical fertilizer year, is the world
Maximum chemical fertilizer importer and nitrogen fertilizer production state.Nevertheless, the need for chemical fertilizer amount does not still meet agricultural development.Study carefully it former
Cause, key issue is that the utilization rate of chemical fertilizer is too low.Department calculates according to the study, and average utilization rate of nitrogen fertilizer in all parts of the country at present is
30-35%, phosphate fertilizer 20~30%, potash fertilizer 30~50%.According to estimates, the whole nation is annual because blindness is using about 1,000,000 tons of chemical fertilizer of waste,
Equivalent 500,000,000 yuan of RMB.In addition due to largely applying chemical fertilizer of simple substance for a long time, the pollution to environment is increasingly severe, soil hardening,
Underground water pollution, quality of agricultural product declines, and has caused the common concern of people.Effective and reasonable use chemical fertilizer, reduces environmental pollution,
Soil hardening is eliminated, soil potential fertility is excavated, chemical fertilizer utilization ratio is improved, the incidence of disease of soil-borne disease is reduced, it has also become pendulum is in agriculture
An important topic in industry production.For the sustainable development of agricultural, high yield, high-quality, efficient purpose, countries in the world are reached
The biological soil conditioner that beneficial microbe all in using soil is made improves the Micro Ecosystem of crop rhizosphere soils,
The material that crops are difficult by is converted into the effective nutriment that can be utilized, and reduces the soil-borne disease incidence of disease, alleviates ecological
The situation of environment adverse effect.
The content of the invention
One of the technical problem to be solved in the present invention is to provide a kind of biological composite soil modifier, its microorganism viable bacteria number
Amount is high, can preserve for a long time at normal temperatures, can be used in mixed way with other organic and inorganic soil conditioners progress unaffected and anti-
Control soil-borne disease effect obvious.
The second technical problem to be solved by the present invention is to provide the preparation method of the biological composite soil modifier.
In order to solve the above technical problems, the present invention provides a kind of biological composite soil modifier, its using compound biological bacterium as
Core, the compound biological bacterium is the combination of Paenibacillus polymyxa and bacillus subtilis.
As currently preferred technical scheme, every gram contains living bacteria count >=1 in the biological composite soil modifier
×109It is individual.
The present invention also provides the preparation method of the biological composite soil modifier, comprises the following steps：
1) soil conditioner is subjected to deep liquid High Density Cultivation with biological bacteria respectively, obtains zymotic fluid, every milliliter of life
Thing fermented liquid contains viable count more than 1 × 1011Individual, zymotic fluid collects bacterium mud after centrifugal treating, adds protective agent and jelly is made
Dry powder；
2) using compound biological bacterium as core, powdery organic fertilizer and perlite stir needed for mixing, screened to be somebody's turn to do
Compound biological soil improver.
As currently preferred technical scheme, the protective agent includes weight and is calculated as according to mixing bacterium mud percentage by weight
5%-40% dusty yeast, weight is 10%-25% algal polysaccharides powder, and weight is 10%-50% skimmed milk power, weight
Measure the inorganic salts for 5%-20%.
As currently preferred technical scheme, described powdery organic fertilizer refers to richnesses such as feces of livestock and poultry, plant and animal residuess
The organic fertilizer that byproduct containing organic matter is made after being become thoroughly decomposed for primary raw material is fermented, the content of organic matter >=30%；Described
The mesh of the fineness of perlite≤40.
The invention has the advantages that：Due to using freeze-dried powder and protectant protection to soil conditioner biological bacteria
Measure, not only makes to be likely to result in the death that the dead link of viable bacteria has prevented viable bacteria body in preparation process, and in the production of shaping
Viable bacteria body has protectant protection in product, may be such that the viable count and survival rate of soil conditioner biological bacteria are significantly improved,
Preserve the biological composite soil modifier of 2 years at normal temperatures, the survival rate of its beneficial bacterium is up to 80%.Because Viable detection is high,
So that the effect of the soil conditioner preventing and treating soil-borne disease containing biological bacteria is obvious.Using soil-borne disease bacterium after compound biological soil improver
Infection rate reduction by more than 50%, and crop yield can be made to increase 15-30%, quality of agricultural product significantly improves.
Embodiment
The invention will be further elaborated by the following examples：
Embodiment 1
LB culture mediums are respectively configured in two 200L fermentation tanks, are sterilized 15-20 minutes at 121 DEG C-125 DEG C, respectively
The Paenibacillus polymyxa of inoculation 10% and bacillus subtilis, are passed through sterile air, every milliliter of work after 40 DEG C of cultures 20 hours
Bacterium number is more than 1 × 1011It is individual, bacterium mud is extracted respectively for 1Kg with 12000 revs/min of rotating speed centrifugation on centrifuge.
By 100g dusty yeasts, 100g algal polysaccharides powder, 200g skimmed milk powers and 20g sodium chloride be compounded into protective agent be equipped with it is suitable
Measure sterilized water, successively add to load after Paenibacillus polymyxa bacterium mud and bacillus subtilis bacterium mud stirring at low speed and emulsification and freeze
In dry care disk, it is freeze-dried in vacuum drier, Paenibacillus polymyxa and bacillus subtilis freeze-dried powder is obtained
2.2Kg。
Using the above-mentioned compound biological bacteriums of 1.0Kg as core, livestock manure fermented organic fertilizer 300Kg and 200Kg perlite is added,
Screened to obtain compound biological soil improver 500Kg, the wherein content of organic matter is 35%, and biologic live bacteria number is 2.5 × 109
Individual/gram.
Embodiment 2
LB culture mediums are respectively configured in two 500L fermentation tanks, are sterilized 15-20 minutes at 121 DEG C-125 DEG C, respectively
The Paenibacillus polymyxa and B. mucilaginocus-frozen glue sample bacillus (Bacillus mucilaginosus) of inoculation 10%, are passed through
Every milliliter of viable count is more than 1 × 10 after sterile air, 40 DEG C of cultures 20 hours11It is individual, with 12000 revs/min on centrifuge
It is respectively 2Kg that bacterium mud is extracted in rotating speed centrifugation.
By 400g dusty yeasts, 300g algal polysaccharides powder, 500g skimmed milk powers and 30g sodium chloride be compounded into protective agent be equipped with it is suitable
Sterilized water is measured, Paenibacillus polymyxa bacterium mud and B. mucilaginocus-frozen glue sample bacillus bacterium mud stirring at low speed and emulsification is successively added
It is fitted into afterwards in lyophilized pallet, is freeze-dried in vacuum drier, Paenibacillus polymyxa and B. mucilaginocus-frozen glue sample is obtained
Bacillus freeze-dried powder 3.0Kg.
Using the above-mentioned biological bacterias of 2.0Kg as core, the mixing of livestock manure fermented organic fertilizer 600Kg and 400Kg perlite is added,
Screened to obtain compound biological soil improver 1000Kg, the wherein content of organic matter is 36%, and biologic live bacteria number is 3.0 × 109
Individual/gram.
Embodiment 3
Paenibacillus polymyxa and the bacterium freeze-dried powder compound biological bacterium of bacillus subtilis two are obtained as described in Example 1
1.2Kg。
Using the above-mentioned compound biological bacteriums of 0.3Kg as core, livestock manure fermented organic fertilizer 150Kg and 150Kg perlite is added
Mixing, screened to obtain compound biological soil improver 295Kg, wherein the content of organic matter is 21%, biologic live bacteria number is 2.6 ×
109Individual/gram.
Beneficial effects of the present invention are further elaborated below by way of test example：
Test example 1
1) this experiment sets two processing altogether：
(1) (habits fertilization) is compareed：Per cell 10Kg commodity composite organic-inorganic fertilizers (content of organic matter 25%, nitrogen, phosphorus,
Potassium total content is 16.5%) to make base fertilizer；(2) per the gained compound biological soil improver of 50 kilograms of above-described embodiments of cell bottom application 1.
In triplicate, in soil property loam in field random alignment, 0.3 mu of plot area, wheat field, soil fertility, field physical features
Flat, category well water is irrigated, wheat breed " 3548 ", the same crop field of field management.
2) test results and analysis：
Wheat growth-promoting is developed, and improves yield, and quality is improved.Winter wheat compound biological soil improver of the present invention
The infection rate reduction by 60% of soil-borne disease bacterium afterwards；And well developed root system, plant strain growth is healthy and strong, and blade is generous, and color is dark green, and it is yellow good to fall, small
Wheat head grain number, mass of 1000 kernel increase, output increased；420.1 kilograms of average yield per mu, compared with 330.0 kilograms of habits fertilization, increase by 90.1 is public
Jin, amount of increase in production 27%.
Test example 2
1) this experiment sets two processing altogether：
(1) (habits fertilization) is compareed：U.S. 15 kgs/acre of 20 kilograms+urea of Ammonium phosphate made in China makees base fertilizer；(2) 60 kilograms/
The gained compound biological soil improver of mu bottom application above-described embodiment 1.Three repetitions, random alignment, 0.1 mu of plot area.Participate in the experiment
Crop：Watermelon (western agriculture 8).
2) fertilizing method：Weigh to mix thoroughly by cell before mulch sowing and make base manure and once concentrate deep placement.
3) field is implemented：Quite, pattern of farming is consistent for basic fertility, field management and local crop field watermelon management method one
Cause.
4) result of the test：
By field observation, watermelon applies the biological composite soil modifier of the present invention compared with the control, comparably, on an equal basis
Under conditions of managerial skills, the infection rate reduction by 75% of soil-borne disease bacterium；Each breeding time climing feelings growing way is vigorous, Fruit is rapid,
The uniform melon of head is big and quality preferably, 9600 pounds/acre of yield increased increases production 2412 pounds/acre, and amplification is up to 25.1%.
Claims (5)
1. a kind of compound biological soil improver, it is characterised in that mainly comprising compound biological bacterium, the compound biological bacterium is many
Viscous series bacillus and the combination of bacillus subtilis.
2. compound biological soil improver according to claim 1, it is characterised in that every gram contains in the compound biological bacterium
Living bacteria count >=1 × 109It is individual.
3. the preparation method of the compound biological soil improver according to claim any one of 1-2, comprises the following steps：
1) biological bacteria used by soil conditioner is subjected to deep liquid High Density Cultivation respectively, obtains zymotic fluid, every milliliter of biology
Fermented liquid contains viable count more than 1 × 1011Individual, zymotic fluid collects bacterium mud after centrifugal treating, and addition protective agent is made lyophilized
Powder；
2) using compound biological bacterium as core, compound life is obtained through screening after powdery organic fertilizer needed for mixing and perlite uniform stirring
Thing soil conditioner.
4. the preparation method of compound biological soil improver according to claim 3, it is characterised in that：The protective agent bag
The dusty yeast that weight is calculated as 5%-40% according to mixing bacterium mud percentage by weight is included, weight is 10%-25% algal polysaccharides
Powder, weight is 10%-50% skimmed milk power, and weight is 5%-20% inorganic salts.
5. the preparation method of compound biological soil improver according to claim 3, it is characterised in that：The powdery organic fertilizer
In, the content of organic matter >=30%；The mesh of the fineness of described perlite≤40.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410581434.5A CN104388093B (en) | 2008-12-25 | 2008-12-25 | A kind of compound biological soil improver and preparation method thereof |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410581434.5A CN104388093B (en) | 2008-12-25 | 2008-12-25 | A kind of compound biological soil improver and preparation method thereof |
| CN200810208210A CN101760202A (en) | 2008-12-25 | 2008-12-25 | Compound biological soil improver and preparation method thereof |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810208210A Division CN101760202A (en) | 2008-12-25 | 2008-12-25 | Compound biological soil improver and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104388093A CN104388093A (en) | 2015-03-04 |
| CN104388093B true CN104388093B (en) | 2017-07-11 |
Family
ID=52606057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410581434.5A Active CN104388093B (en) | 2008-12-25 | 2008-12-25 | A kind of compound biological soil improver and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104388093B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104804745A (en) * | 2015-03-25 | 2015-07-29 | 北京世纪阿姆斯生物技术股份有限公司 | Biological soil conditioner and preparation method thereof |
| CN105016919A (en) * | 2015-07-09 | 2015-11-04 | 潍坊友容实业有限公司 | Soil maturation method for saline-alkaline land using pearlite soil subsoiling agent |
| CN113322079A (en) * | 2021-06-16 | 2021-08-31 | 安康学院 | Soil conditioner capable of preventing and treating soil-borne diseases and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1414093A (en) * | 2002-09-04 | 2003-04-30 | 昆明榕风生物技术有限公司 | Microbial fertilizer bacterial agent for direct decomposing straw as fertilizer and its production process and application method |
| CN1951875A (en) * | 2005-10-20 | 2007-04-25 | 上海拜森生物技术有限公司 | Biological compound fertilizer and its preparing process |
| CN100999667A (en) * | 2007-01-09 | 2007-07-18 | 山东省农业科学院土壤肥料研究所 | Vegetable field soil improving agent and its preparation process and application |
| CN101007957A (en) * | 2007-02-01 | 2007-08-01 | 南京师范大学 | Method for producing biodiesel oil and soil regulator using plant waste |
| CN101126026A (en) * | 2007-10-10 | 2008-02-20 | 中国农业科学院蔬菜花卉研究所 | Vegetable field soil improving agent and its preparation method |
| CN101215543A (en) * | 2008-01-18 | 2008-07-09 | 新疆农业科学院微生物应用研究所 | Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention |
-
2008
- 2008-12-25 CN CN201410581434.5A patent/CN104388093B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1414093A (en) * | 2002-09-04 | 2003-04-30 | 昆明榕风生物技术有限公司 | Microbial fertilizer bacterial agent for direct decomposing straw as fertilizer and its production process and application method |
| CN1951875A (en) * | 2005-10-20 | 2007-04-25 | 上海拜森生物技术有限公司 | Biological compound fertilizer and its preparing process |
| CN100999667A (en) * | 2007-01-09 | 2007-07-18 | 山东省农业科学院土壤肥料研究所 | Vegetable field soil improving agent and its preparation process and application |
| CN101007957A (en) * | 2007-02-01 | 2007-08-01 | 南京师范大学 | Method for producing biodiesel oil and soil regulator using plant waste |
| CN101126026A (en) * | 2007-10-10 | 2008-02-20 | 中国农业科学院蔬菜花卉研究所 | Vegetable field soil improving agent and its preparation method |
| CN101215543A (en) * | 2008-01-18 | 2008-07-09 | 新疆农业科学院微生物应用研究所 | Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104388093A (en) | 2015-03-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104016807B (en) | Special organic microbial fertilizer of a kind of garlic and preparation method thereof | |
| CN100572337C (en) | A kind of whole-fertilizer compound fertilizer and preparation method thereof | |
| CN102816005B (en) | Peanut special microbial fertilizer and production method thereof | |
| CN101760202A (en) | Compound biological soil improver and preparation method thereof | |
| CN100488919C (en) | Biological compound fertilizer and its preparing process | |
| CN104045469B (en) | A kind of biological organic fertilizer being exclusively used in saltings and its preparation method and application | |
| CN100460365C (en) | Nano biological organic and inorganic composite fertilizer | |
| CN104672033A (en) | Amino acid bioorganic fertilizer as well as preparation method and use of fertilizer | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN108752135A (en) | A kind of Chinese medicine special bio bacterial manure and the preparation method and application thereof | |
| CN105085111A (en) | Biological-organic fertilizer special for corn and preparation method thereof | |
| CN105199962A (en) | Microbial straw-decomposing agent as well as preparation method and application thereof | |
| CN106396941A (en) | Novel compound microorganism fertilizer | |
| CN106316687A (en) | Organic fertilizer giving consideration to crop nutrient needs and soil ecological improvement and preparation method thereof | |
| CN110143836A (en) | Using phosphoric acid as regulating acid agent and the rice seedling-growth soil regulator of phosphorus source and its preparation method and application | |
| CN105330420A (en) | Biological function organic-inorganic compound fertilizer containing amino acid, humic acid and nicotine and preparation method of biological function organic-inorganic compound fertilizer | |
| CN106190929B (en) | A kind of new strain of Bacillus licheniformis and its application | |
| CN116253600B (en) | Organic fertilizer and method for promoting vegetable growth and/or preventing and controlling vegetable diseases | |
| CN112457137A (en) | Amino acid fertilizer for improving saline-alkali soil and application thereof | |
| CN102173887A (en) | Production method of biological organic fertilizer containing Avermectins | |
| CN109438057A (en) | A kind of humic acid composite material and preparation method thereof | |
| CN108821811A (en) | It is a kind of using stalk as composite microbe fertilizer of raw material and preparation method thereof | |
| CN106495946A (en) | A kind of Arenga pinnata tree long-acting specific composite fertilizer material and preparation method thereof | |
| CN105037042A (en) | Bio-fertilizer prepared through complex microbial inoculant secondary aerobic fermentation and preparation method thereof | |
| CN104388093B (en) | A kind of compound biological soil improver and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20211222 Address after: 201201 No. 9, Huiqing Road, Pudong New Area, Shanghai Patentee after: SHANGHAI HEQING DRAGON FRUIT TECHNOLOGY DEVELOPMENT CO.,LTD. Address before: 201210 b2-5-1, No. 185, Mian North Road, Sunqiao, Pudong New Area, Shanghai Patentee before: Shanghai Baisn Organic Agriculture Development Co.,Ltd. |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20150304 Assignee: Shanghai Yingmao Industrial Co.,Ltd. Assignor: SHANGHAI HEQING DRAGON FRUIT TECHNOLOGY DEVELOPMENT CO.,LTD. Contract record no.: X2022980014132 Denomination of invention: A kind of composite biological soil conditioner and preparation method thereof Granted publication date: 20170711 License type: Common License Record date: 20220906 |