CN104387505B - The extracting method of xylan in a kind of spirit distiller grain - Google Patents
The extracting method of xylan in a kind of spirit distiller grain Download PDFInfo
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- CN104387505B CN104387505B CN201410620283.XA CN201410620283A CN104387505B CN 104387505 B CN104387505 B CN 104387505B CN 201410620283 A CN201410620283 A CN 201410620283A CN 104387505 B CN104387505 B CN 104387505B
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- 229920001221 xylan Polymers 0.000 title claims abstract description 56
- 150000004823 xylans Chemical class 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 53
- 239000003513 alkali Substances 0.000 claims abstract description 18
- 238000001556 precipitation Methods 0.000 claims abstract description 18
- 230000008569 process Effects 0.000 claims abstract description 18
- 238000012545 processing Methods 0.000 claims abstract description 15
- 238000006386 neutralization reaction Methods 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 19
- 239000006228 supernatant Substances 0.000 claims description 14
- 239000012043 crude product Substances 0.000 claims description 12
- 239000002244 precipitate Substances 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 239000003960 organic solvent Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 235000020097 white wine Nutrition 0.000 claims description 2
- 150000001298 alcohols Chemical class 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 9
- 238000011084 recovery Methods 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 238000004064 recycling Methods 0.000 abstract description 4
- 229920002488 Hemicellulose Polymers 0.000 description 22
- 238000000605 extraction Methods 0.000 description 16
- 239000002253 acid Substances 0.000 description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 8
- 229920005610 lignin Polymers 0.000 description 7
- 239000001913 cellulose Substances 0.000 description 6
- 229920002678 cellulose Polymers 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 235000019441 ethanol Nutrition 0.000 description 5
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- 241000196324 Embryophyta Species 0.000 description 4
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 4
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- -1 compound amino acid Chemical class 0.000 description 3
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- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 2
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- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 2
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- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
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- 229930006000 Sucrose Natural products 0.000 description 1
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- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 1
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Abstract
The present invention provides a kind of extracting methods of xylan in spirit distiller grain, it is characterised in that includes the following steps：（a）It is 6 ~ 7 to clean to pH；（b）Drying；（c）It crushes；（d）High temperature high pressure process；（e）Alkali process；（f）Neutralization precipitation.What the present invention solved that wine brewing generates in the process loses poor recycling problem, and step specification, feasibility height, safety non-pollution, low cost, and can bring great economic benefit, will be very beneficial for losing grain recovery processing and recycling.
Description
Technical field
The present invention relates to natural extraction fields, more particularly to a kind of extracting method of xylan in spirit distiller grain.
Background technology
It is the inevitable outcome during brewed spirit to lose grain, often produces 1 ton of white wine and loses grain it is necessary at least generate 6 tons, thus
Estimation only Sichuan year generates 20,000,000 tons or so of Lost grains.Due to losing poor water content 55% or so, it is difficult in dry matter in addition point
The substance proportion of solution is also in 60% or more organic acid of the , Lost grains containing higher concentration, humus etc., it is difficult to store, exposure
It is easy to go rotten in air, all have a certain impact to air, water body, soil, the recovery processing of Suo Yi Lost grains is particularly important.
With developing for the industry that brews alcoholic beverages, the processing cost for losing grain also and then skyrockets in recent years, per ton at 800 yuan or so,
The direct processing for causing certain financial burden to liquor-making enterprise, and losing grain also results in the waste in resource.If energy
The mode for losing poor economical rationality is recycled, tell on significant economic value, and solution of can yet be regarded as is discarded to lose grain
A good approach.
Research of the China in terms of losing poor comprehensive utilization has been achieved for certain achievement, for example, the profits such as Qin Yunan, quick
Glycerine is produced with vinasse；Lee's virtue, Liu Guiyun, Xu Ruyi etc. utilize vinasse culturing edible fungus；Jiang Ying, yellow the U.S. and Britain etc. are from vinasse
Extract compound amino acid and trace element；Gu Hongbang, history build Pei etc. and extract phytic acid and Phytin from vinasse；Model essay power, quotient's turbulent waves
Vinegar is processed Deng with fresh grain stillage；Li Xiangqian, king quite etc. with biotechnology processing vinasse-solid state fermentation production mycoprotein and
Cellulase；Zhao Shouxian, Zhang Hongbin etc. produce cellulolytic enzyme using vinasse；Bright conjunction of Hua Songjin, Xue Li, fourth etc. utilizes vinasse
Anaerobic fermentation recycles biogas；Jiang Hai etc. uses ZnCl2Activation method vinasse husk produces craboraffin；The liquid such as Ding Minghe, Wang Chenghui
Body vinasse culture Dipel；Wang Binyong loses grain and makees dyeing reducing agent with lime fermentation；Sieve is kind to help that grain is lost in external application and Chinese medicine is controlled
Treat rheumatoid arthritis；Shen Xiaokun will lose the present situation that grain solves animal protein feed shortage for fly maggot breeding-；Recently
Also it has been reported that using vinasse as raw material and produces aminobutyric acid I etc. with Lactobacillus brevis, but because above-mentioned lose grain recovery processing complexity, work
Skill is lengthy and tedious, yield is relatively low, and return is little, only theoretical research at present, no actual application value, and most of brewery still will lose grain
Farmers' is sold to as feed, agricultural fertilizer.
The basic chemical composition for losing main component in grain is：Moisture 7.5%~15%, crude fibre 35.5%~45%, wood
Quality 21%~26%, crude protein 25%~30%, pentosan 16%~22%, ash content 13%~22%.Wherein mostly contracting penta
Sugar produces xylan through mitigating to hydrolyze, and xylan is a kind of five-carbon ring aldehydo sugar, is white crystalline powder, pleasantly sweet, sugariness is suitable
In the 40% of sucrose, the 50% of beet sugar, the sweetener of diabetic can be used as, food the effect of hepatitis patient, due to wood
Glycan has prodigious reaction vigor, can generate a series of valuable derivatives, be also widely used for food, chemistry, leather etc.
Industrial department.Currently, the xylan source of goods is in great shortage in the world, disparities between supply and demand are very prominent, the price of xylan product is up to 24~
260000 yuan/ton, therefore, losing grain production xylan has quite high economic benefit, and can greatly reduce and lose grain to environment
Caused by pollute, have certain environment protection significance.
The chemical structural formula of xylan is as follows：
There are different degrees of combinations between xylan and cellulose, lignin in plant material.Now it has proven convenient that xylan
It is by being covalently keyed with lignin, specific connection type is：The arabinose residues and glucuronic acid of hemicellulose are residual
Base is linked together with the phenyl ring of lignin by covalent bond.Xylan may also have chemical key connection with other polysaccharide such as pectin
Effect.
Extraction xylan will be hydrolyzed first so that it becomes oligosaccharide or monosaccharide, but in cell wall in many cases
In plant tissue biosynthesis, cell differentiation occurs in forming layer, cell interior is plasm, at the beginning of outside is one layer of hemicellulose
Raw wall film forms second layer cell wall with the growth of cell, it is substantially only made of cellulose, the life week of cell
Phase terminates, subsequent apoptosis with the lignified completion of plant tissue.The biosynthesis of lignin is in intercellular layer
It is carried out in hemicellulose and second layer cell wall, so cellulose, hemicellulose and lignin component are in discontinuous stratiform
Structure has inhibition to the hydrolysis of acid or alkali.Cellulose and hemicellulose is set effectively to hydrolyze and then be fully used,
Plant material must be pre-processed, destroy cell wall structure, to be suitble to the attack of acid and enzyme.
The xylan in raw material mainly is extracted by three kinds of cooking process, acid-hydrolysis method, alkali hydrolysis method methods both at home and abroad, with
And propose in recent years steam-explosion method, ultrasonic extraction extraction xylan, each method advantage and disadvantage are as follows：
(1) acidity extraction：Hemicellulose can be preferably dissolved in diluted acid.When with dilute acid pretreatment, hemicellulose simultaneously by
It degrades in the effect of acid.Hemicellulose dilute acid hydrolysis is actually to be carried out under dissolved state.Diluted acid extracts hemicellulose
Xylan, which is substantially all, when plain is hydrolyzed into monosaccharide i.e. xylose, thus cannot be used for the production of xylo-oligosaccharide.Acidity extraction xylan
It is successfully used for xylose production.But extraction xylan haves the shortcomings that larger, for example xylose ratio is very high in extracting solution, Bu Nengman
The production requirement of sufficient xylo-oligosaccharide；It will produce many side reactions in extraction process and generate some possible carcinogens, from
And influence the safety of finished product.
(2) alkali extraction method：Its principle is to be dissolved in aqueous slkali using hemicellulose, and the property insoluble in other solvents is first used
Aqueous slkali extracts hemicellulose；Then precipitation extraction object obtains xylan.The method is suitable for
The hemicellulosic material of main component.The advantages of method is that yield is higher, DNA purity is high；The disadvantage is that usually to raw material without pre-
Processing or pre-cooked temperature are inadequate, therefore do not have the effect that compound combines that destroys, to influence yield.
(3) high-temperature cooking process：Using the combination of high temperature xylan and cellulose, lignin, contained using xylan itself
Some acetyl group fall off under the action of certain temperature or pressure generates acetic acid, and the pH value of system is caused to reduce, to keep wood poly-
Itself hydrolysis occurs for sugar, and xylan molecular weight reduces, solubility increases.The advantages of method is that treating capacity is larger, yield compared with
Height, but DNA purity is not as good as alkali extraction method, and cooking process needs higher temperature and pressure, thus it is higher to equipment requirement.
(4) steam-explosion method：Under certain pressure, temperature and moisture condition, glycosidic bond fracture occurs for hemicellulose, releases
Acetic acid is released, that is, carries out itself hydrolysis, becomes that molecular weight is smaller, water-soluble xylan.The advantages of method is processing
Amount is big, and in Canada, oneself is applied to commercially produce this preconditioning technique, and shortcoming is to the more demanding of equipment, energy
Consume it is larger, under the high temperature conditions since the denaturation of part xylose will produce the harmful substances such as furfural.
(5) ultrasonic extraction：The shadow of each factor to yield when having investigated with ultrasonic extraction method xylan such as Yang Jian
It rings, and the optimal conditions of extraction process is determined by orthogonal experiment, Zdena etc. compared traditional extraction process and ultrasonic wave carries
Take the influence to xylan yield.Its shortcoming is that processing is complicated, and technique is lengthy and tedious, is unfavorable for commonly promoting the use.
Therefore, if there is a kind of step specification, feasibility height, safety non-pollution, low cost, tremendous economic can be brought to imitate
Benefit loses the method that xylan is extracted in grain, will be very beneficial for losing grain recovery processing and recycle.
Invention content
The present invention is directed to above-mentioned present situation, provides a kind of extracting method of xylan in spirit distiller grain, solves and loses grain
Recycling problem, and step specification, feasibility height, safety non-pollution, low cost, can bring great economic benefit, will
It is very beneficial for losing grain recovery processing and recycles.
Purpose to realize the present invention, the technical solution adopted by the present invention are as follows：
The extracting method of xylan in a kind of spirit distiller grain, it is characterised in that include the following steps：
(a) it is 6~7 to clean to pH；
(b) it dries；
(c) it crushes；
(d) high temperature high pressure process；
(e) alkali process；
(f) neutralization precipitation.
Further, in (b) baking step, the grain of losing after cleaning is divided in baking oven, 85~95 DEG C of drying 1~
2 hours.
Further, in (c) pulverising step, the grain of losing after drying is crushed into processing, granularity is more than or equal to 60
Mesh.
Further, (d) the high temperature high pressure process step is that will lose grain after crushing, with high-tension apparatus 115~
125 DEG C, 1~2h is handled under 0.1~0.2MPa.
Further, in (e) alkali treatment, grain washing filtering will be lost after high temperature high pressure process, until pH value is 6
~7；It is 14.4~14.7 that lye, which is added, to pH, is heated to 85~95 DEG C of 4~6h of constant temperature.
Further, the sodium hydroxide or potassium hydroxide solution that the lye is 10%~15%；(e) in alkali treatment
Solid-to-liquid ratio is 1：10~15.
Further, in (f) the neutralization precipitation step, the grain separation of losing after alkali process is taken into supernatant, it is 4 to be adjusted to pH
~4.5, temperature is reduced to 1~6 DEG C of 6~12h of constant temperature, and it is xylan crude product that separation, which takes precipitation,.
Further, in a kind of spirit distiller grain xylan extracting method, further include that step (g) organic solvent is heavy
It forms sediment, by neutralization precipitation, treated loses grain and be separated into supernatant and precipitate A；Supernatant is taken to carry out two using water-soluble organic reagent
Secondary precipitation detaches to obtain precipitate B；It is xylan crude product that precipitate A and precipitate B, which are mixed,.
Further, it in step (g) organic solvent deposit, takes neutralization precipitation treated and lose grain separation supernatant tune
It is 4.5~5.5 to pH, alcohol reagent is added, precipitate 50~70min, supernatant is 1 with alcohol reagent volume ratio：2.5~3.5.
The present invention also provides a kind of methods of purification of the xylan crude product extracted in spirit distiller grain, it is characterised in that including
Following steps：Xylan crude product is heated to 75~80 DEG C, is stirred after adding activated carbon, continues 15~20 minutes, is filtered；It will filtering
Xylan crude product temperature is down to 30~40 DEG C afterwards, first passes through the processing of 723 type strong acidic ion resins, porous using strong basicity
Resin anion (R.A.) processing after to get.
The principle of the present invention is as follows：
(1) lose grain do not clean before：PH=5.5 or so of the grain before not cleaning is lost, this is because losing grain during the fermentation
Produce part organic acid, it can be such that the hemicellulose lost in grain is largely dissolved in diluted acid, it is simultaneous degradation due to
Acid solutions are relatively low to be ignored, and the extraction of xylan is conducive to, and recovery rate increase is found more when being compared with raw materials for production
Obviously.
(2) it is 6~7 to clean to pH：Acid solution is cleaned, makes to lose grain holding neutrality, be avoided that during crushing high temperature and pressure,
Organic acid continue deep degree with lose it is poor react, generate more monosaccharide and side reaction, be unfavorable for the extraction of xylan.
(3) it dries, crush：So that the specific surface area for losing grain is increased, can be sufficiently mixed with water, lye, it is poly- to be more advantageous to wood
The extraction of sugar；
(4) high temperature high pressure process：The boiling of high temperature and certain pressure can realize that the destruction combined with lignin to xylan is made
With making xylan separate out；
(5) alkali process：Hemicellulose is extracted using hemicellulose dissolubility, while eliminating cellulose etc. and does not dissolve in alkali
Impurity；
(6) neutralization precipitation：It is settled out the neutral fraction i.e. hemicellulose A of higher molecular；
(7) organic solvent deposit：Supernatant organic solvent deposit after neutralization precipitation goes out xylan in hemicellulose B,
And merge precipitation, and it washes, it is dry, improve yield；
(1971, famous chemist Blake etc. suggested the hemicellulose that alkali carries take being divided into two components：Alkali extract is used
After acetic acid neutralizes, what is precipitated when pH is 5.0 is known as hemicellulose A, and what is precipitated in the acid solution of upper layer plus after ethyl alcohol or acetone is known as
Hemicellulose B, so hemicellulose A, hemicellulose B are a kind of mixing polysaccharides.)
(8) it purifies：Xylan crude product passes through activated carbon decolorizing, and multiple column chromatography obtains the higher finished product of purity.
Compared with prior art, the present invention its difference and advantageous effect are shown：
Beneficial effects of the present invention are shown：
One, the present invention provides a kind of practicable spirit distiller grains to recycle new method, solves current wine-making industry
In lose grain recycling problem.
Two, xylan product yield of the present invention is compared traditional technology and is significantly improved, and crude product yield is about 29.1%, and sterling obtains
Rate is about 18.5%.
Three, safety of the present invention is higher, does not have side reaction to generate harmful substance or high-temperature denatured show in extraction process
As existing, and it is environmentally friendly, do not influence operator's health.
Four, the present invention is at low cost, and operates and simplify specification, while can generate huge economic benefit, and being beneficial to promote makes
With.
Description of the drawings
Fig. 1 is the process flow chart of the extracting method of xylan in a kind of spirit distiller grain of the present invention.
Specific implementation mode
One, pre-processing：It takes 10 grams to lose grain, cleans straight pH=6~7 or so, divide the grain of losing after cleaning in baking oven,
90 DEG C dry 1 hour.It is crushed, is then taken with the sieve of 60 mesh sieve spare with high speed disintegrator；
Two, xylan crude product extracts：10.7% 130 milliliters of sodium hydroxide processing pre-processing it is good lose grain, be placed in 90 DEG C
5 hours are heated in thermostat water bath；7000 revs/min of centrifugations take supernatant, with 38% hydrochloric acid tune pH=4.2, place 4 DEG C
Refrigerator overnight generates precipitation；7000 revs/min of centrifugations take precipitation, are washed with distilled water to native white, A are obtained, by separation
It is 4.5~5.5 that supernatant, which is adjusted to pH, and alcohol reagent is added, and precipitates 50~70min, and supernatant is 1 with alcohol reagent volume ratio:
3, B is obtained, merges A+B, washing, 37 DEG C dry to obtain crude product, weigh and label, are put into drier.
Three, xylan crude product decolourizes：Syrup temp is first increased to 75~80 DEG C when decoloration, then adds activated carbon, and even
Continuous stirring, prevents activated carbon from sinking to the bottom.In acid condition, the good decolorizing effect of activated carbon.Decoloration 15~after twenty minutes, material is saturating
For lightness up to 30~40%, purity is also reusable after regenerating active carbon up to 75~85%.
Four, xylan sterling is produced：723 type strong acidic ion resins (to remove remaining ash content) and the porous the moon of strong basicity
Ion exchange resin (to reduce remaining acidity), the two will match.Operating process is the liquid glucose temperature drop that will be filtered out after purification
To 30~40 DEG C, first through cation exchange resin, remaining ash content 90% or so is removed, anion exchange resin is then passed through.This
When be combined and removed with the organic acid flowed out by anion exchanger by the inorganic acid of cation exchanger outflow, at this time in
2~3% can be improved again with the purity of liquid.After quantitative material finishes, two kinds of resins are rinsed until without sugar with tap water.So
It is backwashed from bottom to top with tap water again afterwards, the resin layer in ion-exchanger is made loosely to stir, removed by overflow
Layer impurity, then regenerated with acid & alkali liquid, finally be originally washed to water be in it is as clear as crystal until.
Claims (7)
1. the extracting method of xylan in a kind of spirit distiller grain, it is characterised in that include the following steps：
(a) it is 6~7 to clean to pH；
(b) it dries；
(c) it crushes；
(d) high temperature high pressure process；
(e) alkali process；
(f) neutralization precipitation；
(d) high temperature high pressure process step is that will lose grain after crushing, with high-tension apparatus at 115~125 DEG C, 0.1~
1~2h is handled under 0.2MPa；
(e) alkali treatment is grain washing filtering will to be lost after high temperature high pressure process, until pH value is 6~7；Lye is added
It is 14.4~14.7 to pH, is heated to 85~95 DEG C of 4~6h of constant temperature.
2. the extracting method of xylan in a kind of spirit distiller grain according to claim 1, it is characterised in that：(b) dries
In dry step, the grain of losing after cleaning is divided in baking oven, 85~95 DEG C dry 1~2 hour.
3. the extracting method of xylan in a kind of spirit distiller grain according to claim 1, it is characterised in that：(c) powder
During quick short steps are rapid, the grain of losing after drying is crushed into processing, granularity is more than or equal to 60 mesh.
4. the extracting method of xylan in a kind of spirit distiller grain according to claim 1, it is characterised in that：The lye is
10%~15% sodium hydroxide or potassium hydroxide solution；(e) solid-to-liquid ratio is 1 in alkali treatment：10~15.
5. the extracting method of xylan in a kind of spirit distiller grain according to claim 1, it is characterised in that：In (f)
In settling step, the grain separation of losing after alkali process is taken into supernatant, it is 4~4.5 to be adjusted to pH, reduces temperature to 1~6 DEG C of constant temperature
6~12h, it is xylan crude product that separation, which takes precipitation,.
6. the extracting method of xylan in a kind of spirit distiller grain according to claim 1, it is characterised in that：It is described a kind of white
Wine loses the extracting method of xylan in grain, further includes step (g) organic solvent deposit, and by neutralization precipitation, treated loses poor separation
For supernatant and precipitate A；It takes supernatant to carry out secondary precipitation using water-soluble organic reagent, detaches to obtain precipitate B；By precipitate A and
Precipitate B mixing is xylan crude product.
7. the extracting method of xylan in a kind of spirit distiller grain according to claim 6, it is characterised in that：The step
(g) in organic solvent deposit, it is 4.5~5.5 to take neutralization precipitation treated and lose grain separation supernatant to be adjusted to pH, and alcohols examination is added
Agent, precipitates 50~70min, and supernatant is 1 with alcohol reagent volume ratio：2.5~3.5.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101935717A (en) * | 2010-08-23 | 2011-01-05 | 吉林大学 | Method for efficiently separating xylose from beer lees |
| CN103614435A (en) * | 2013-12-02 | 2014-03-05 | 广西壮族自治区农业科学院农产品加工研究所 | Method for preparing xylo-oligosaccharide from bagasse |
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| CN101935717A (en) * | 2010-08-23 | 2011-01-05 | 吉林大学 | Method for efficiently separating xylose from beer lees |
| CN103614435A (en) * | 2013-12-02 | 2014-03-05 | 广西壮族自治区农业科学院农产品加工研究所 | Method for preparing xylo-oligosaccharide from bagasse |
Non-Patent Citations (2)
| Title |
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| 稻壳预处理及稻壳中木聚糖的提取工艺研究;韩丹妮;《中国优秀硕士学位论文全文数据库》;20121015;正文第37-38页 * |
| 超声波辅助碱法提取酒糟中纤维素和半纤维素;任海伟等;《农产品加工·学刊》;20121130(第11期);第34-38,42页 * |
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