CN104381134B - A kind of method of Herba Thymi fast breeding - Google Patents
A kind of method of Herba Thymi fast breeding Download PDFInfo
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- CN104381134B CN104381134B CN201410745993.5A CN201410745993A CN104381134B CN 104381134 B CN104381134 B CN 104381134B CN 201410745993 A CN201410745993 A CN 201410745993A CN 104381134 B CN104381134 B CN 104381134B
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Abstract
A kind of method that the invention discloses Herba Thymi fast breeding, including the flower pesticide disinfection taking mid-late uninucleate stage；Inoculation callus inducing medium, cultivates and obtains callus；Subculture multiplication is cultivated, proceed to non-staining callus the first subculture medium makes Shoot propagation, subculture 4 times, proceed to the second subculture medium or without subculture in the blank cultures of any hormone once, proceed to the first subculture medium continues propagation the most again, according to needing Seedling amount repeat the above steps；Inoculation root media, screening obtains monomer plant.The cultivation results of the present invention is: the inductivity of callus reaches 89%, and rooting rate reaches 85%, and transplanting survival rate reaches 80%, and 10 generation aberration rates are below 6%.
Description
Technical field
The invention belongs to agricultural technology field, relate to tissue culture technique, be specifically related to a kind of ground
The method of green pepper fast breeding.
Background technology
Herba Thymi, has another name called ground Pericarpium Zanthoxyli (" marine recipe "), Herba thymi vulgaris (" botany voluminous dictionary "),
Pericarpium Zanthoxyli (" illustrated handbook planted by middle traditional Chinese medicines "), Fructus Linderae Glaucae (" Liaoning economic plants will ").For Labiatae
Plant.It is distributed mainly on the ground such as Inner Mongol, Ningxia, Qinghai, Shanxi.In application in TCM permissible
Expelling pathogenic wind from the body surface, promoting the circulation of QI to relieve pain, it is used for catching a cold, has a headache, has a toothache, abdominal distention cold type of pain.
Herba Thymi Herb has strong fragrance, adopts several leaves, rubs in hands, so that it may send light
Light fragrance, fragrance gentleness can people.Aromatic oil accounts for the 3% of the stem tip and leaf, and main component has virtue
Radix Cinnamomi porrecti alcohol, Borneolum Syntheticum, to a P-cymene, thymol, amine oleyl alcohol, tannin Bulbus Allii, saponin,
Bitter substance and various mineral, wherein potassium, calcium content are higher.There is good beauty treatment, guarantor
Stiff anticorrosion, sterilize, help digest, relieve the effect of alcohol, diuresis, the effect such as rheumatism, its special feature
It is can strengthening immune system.Therefore Herba Thymi has highly important medical value.Herba Thymi medicinal
It is worth the most on the books in Compendium of Material Medica.In the medical research of the former Soviet Union, to its leaf
The antisepsis and sterilization characteristic of flower is the most on the books.In recent years, by the chemical composition of Thymus plant
For medical treatment, keep healthy, make up, food, its potential value developed becomes clear day by day.Mesh
Before, Herba Thymi is wild mostly, is underutilized.
Herba Thymi can be sowed, cuttage, press strip, offshoot breeding, in addition to summer, the most very
It is suitable for breeding.But these propagation method speed are slower, it is difficult to reach the purpose of fast breeding.
For excavating its medical value, obtain a large amount of aseptic seedling in a short time with tissue culture quick propagation culturing method
Prospect for the effective medicinal ingredient of extraction is possible." tissue culture of Herba Thymi and plant are again
Raw " in (Zhao Qingzhen, Plant Physiology Communications, the 4th phase of volume 38, in August, 2002) literary composition
Disclose and carry out tissue culture quickly by the tender stem section of band axillalry bud as vegetative material
The method obtaining ground pepper plant, " Herba Thymi tissue culture and prevent vitrification from studying " (Xu Meilong,
Agriculture of Anhui science, Journal of Anhui Agri.Sci.2009,37 (1): 114-116) literary composition
In also disclose that the stem section of band axillalry bud carries out tissue culture and to it as vegetative material
Condition of culture is screened, although breeding potential is the highest, but inventor is in long-term operation
During find easily to occur after continuous successive transfer culture 4 generation variation, accordingly, it would be desirable to not disconnecting
Plant cultivation acquisition callus and could breed more plant, unfavorable to Fast-propagation.
Summary of the invention
For the problem that presently, there are, the present invention provides a kind of method of Herba Thymi fast breeding, can
To breed Herba Thymi fast and effectively, and solving the problem that its successive transfer culture makes a variation, survival rate is relatively
Height, is not affected by season, and propagation cost is greatly lowered, and meets large-scale production needs.
In order to solve the problems referred to above, the present invention provides following technical scheme:
A kind of method of Herba Thymi fast breeding, including
1) the flower pesticide disinfection of mid-late uninucleate stage is taken；
2) inoculation callus inducing medium, cultivates and obtains callus；
3) subculture multiplication is cultivated, and is proceeded in the first subculture medium by non-staining callus
Make Shoot propagation, subculture 4 times, proceed to the second subculture medium or the blank without any hormone
In culture medium, subculture is once, proceeds to continue in the first subculture medium propagation the most again, according to need
Seedling amount repeat the above steps；
4) inoculation root media, screening obtains monomer plant.
Described disinfects the wine being flower pesticide sterile gauze parcel immerses 70-75% concentration
Sterilize in essence 8-16s, quickly removes to immerse immediately and soaks 10min in bleaching powder, finally with aseptic
Water rinses 4～6 times.
Described callus inducing medium is: 1/2MS+6-BA 1.2mg/L+NAA
0.12mg/L+ sucrose 50g/L；The PH6.6 of culture medium, cultivation temperature is 25 ± 1 DEG C, 12h/d
Photoperiod, about intensity of illumination 1700Lx.
The first described subculture medium is 1/2MS+6-BA1.0mg/L+2,4-D
0.2mg/L+NAA 0.3～0.5mg/L+ agar 3g/L+ sucrose 15g/L；Described second subculture training
Foster base is 1/2MS+6-BA 2mg/L+NAA 0.1mg/L+ white sugar 35g/L+ agar powder
7.3g/L；Described blank cultures is 1/2MS+ potassium dihydrogen phosphate 150mg/L+ lactoalbumin hydrolysate
500mg/L+ sucrose 30g/L, in Subculture, the PH6.5 of culture medium, cultivation temperature is
25 ± 1 DEG C, the photoperiod of 16h/d, about intensity of illumination 1200Lx.
Described root media is: 1/2MS+IAA 0.1～0.3mg/L+ sucrose 20～
30g/L+ agar 2～3g/L+ quality of activated carbon ratio 0.1%～0.3%；Condition of culture is: cultivate
The PH6.5 of base, cultivation temperature is 25 ± 1 DEG C, the photoperiod of 12h/d, intensity of illumination 1600Lx
Left and right.
Incubation also includes, acclimatization and transplants: first culture bottle is moved to greenhouse, natural bar
Part lower refining seedling 4d, healthy and strong until Herba Thymi Seedling and when having 5-7 sheet true leaf, 6-8cm height, then solve Kaifeng
Membrana oralis, seedling exercising 6d, seedling exercising takes out seedling after completing and cleans the culture medium of root attachment, is transplanted to
In sterilized substrate, temperature controls at 24-26 DEG C, front 7d humid control about 70%,
It is gradually lowered humidity later, until transplanted seedling is placed under natural conditions, suitable during acclimatization and transplants
Work as shading, make intensity of illumination be nature light 50%.
Inventor proves effectiveness of the invention by series of experiments.
(1) selection of outer implant
In 5, June, the flower pesticide gathered, the stem section of band axillalry bud, stem apex, petal are made respectively
Contrasting for outer implant, remaining condition is identical, and carries out according to the step of the present invention, sees
Table 1, from contrast it can be seen that employing flower pesticide is as outer implant, the not only induction of callus
Rate and rooting rate are the highest, can reach 80%, 76% respectively, and through 5 generation successive transfer culture
Aberration rate is minimum, can solve the difficult problem easily occurring variation in Herba Thymi reproductive process.
Table 1
(2) selection of successive transfer culture culture medium
Method 1: 50 calluss are inoculated into 1/2MS+6-BA1.0mg/L+2,4-D
In 0.2mg/L+NAA 0.3～0.5mg/L+ agar 3g/L+ sucrose 15g/L culture medium, continue
Culture medium is not the most changed during culture.
Method 2: other 50 calluss proceed to make Shoot propagation in the first subculture medium, continue
Generation 4 times, proceed to the second subculture medium subculture once, proceed to the first subculture medium the most again
Middle continuation breeds, and the first subculture medium is 1/2MS+6-BA1.0mg/L+2,4-D
0.2mg/L+NAA 0.3～0.5mg/L+ agar 3g/L+ sucrose 15g/L；Second subculture medium
For 1/2MS+6-BA 2mg/L+NAA 0.1mg/L+ white sugar 35g/L+ agar powder 7.3g/L.
Method 3: also have 50 calluss to proceed to make Shoot propagation in the first subculture medium, continue
Generation 4 times, proceed to subculture in the blank cultures without any hormone and once, proceed to the most again
First subculture medium continues propagation.First subculture medium is
1/2MS+6-BA1.0mg/L+2,4-D 0.2mg/L+NAA 0.3～0.5mg/L+ agar 3g/L+
Sucrose 15g/L；Blank cultures is 1/2MS+ potassium dihydrogen phosphate 150mg/L+ lactoalbumin hydrolysate
500mg/L+ sucrose 30g/L.
Comparing its aberration rate after 10 generation successive transfer culture, comparing result is shown in Table 2, from comparing knot
From the point of view of Guo, after 4 cultures, starting Transition medium, its aberration rate can greatly reduced,
Herba Thymi fast breeding had conclusive effect.
Table 2
| Contrast | Aberration rate (%) |
| Method 1 | 43 |
| Method 2 | 4% |
| Method 3 | 6% |
Beneficial effect:
(1) the application operates through a large amount of long-term practice using flower pesticide as outer implant, inventor,
Find as outer implant, flower pesticide can be kept its excellent character for a long time, be not likely to produce variation；
(2) inventor find, during industrial seedling rearing, subculture more than 4 generations about,
Start that variation occurs, it has been investigated that, above-mentioned reason is that hormone accumulation causes, and therefore, is continuing
During Dai, the method that have employed Transition medium, reduces the accumulation of hormone to the shadow of Herba Thymi Seedling
Ringing, this is also one of the emphasis of the present invention；
(3) in whole incubation, 1/2MS culture medium is all used to replace conventional MS
Culture medium, it is to avoid tissue cultured seedling excessive growth affects Bud polarization；Blank cultures adds and can promote
Enter potassium dihydrogen phosphate and the lactoalbumin hydrolysate of Bud polarization；During subculture, improve light intensity,
Culture medium decreases inorganic salt content, adds potassium dihydrogen phosphate and lactoalbumin hydrolysate, thus
Inhibit tissue cultured seedling excessive growth, promote Bud polarization, achieve unexpected effect；
(4) present invention have pollen germination become that the embryo time is short, doubling etticiency is high, yield is big and
It is easier to obtain the advantages such as good breeding intermediate materials, in breeding material purification and rejuvenation, plants matter money
The aspect contents such as source innovation, polyploid breeding and pollen development process research, have bigger reality
By value；
(5) incubation speed of the present invention is fast, and regeneration frequency is higher, and aberration rate is relatively low, week
Phase is relatively short, can preferably keep the hereditary stability of Herba Thymi.
Detailed description of the invention
Embodiment 1: a kind of method of Herba Thymi fast breeding, including
1, the selection of outer implant: by the end of June at full-bloom stage, 9:00～10:00 in the morning) take monokaryon
Keep to the side the flower pesticide of phase.
2, disinfect: flower pesticide sterile gauze parcel is immersed in the ethanol of 70-75% concentration
Sterilization 8-16s, quickly removes to immerse immediately and soaks 10min in bleaching powder, finally rush with sterilized water
Wash 4～6 times.
3, inoculated and cultured: the outer implant after disinfecting is inoculated callus induction training
Support base, cultivate and obtain callus.Callus inducing medium is: 1/2MS+6-BA
1.2mg/L+NAA 0.12mg/L+ sucrose 50g/L；The PH6.6 of culture medium, cultivation temperature is
25 ± 1 DEG C, the photoperiod of 12h/d, about intensity of illumination 1700Lx.
4, subculture multiplication is cultivated, and is proceeded in the first subculture medium by non-staining callus
Make Shoot propagation, subculture 4 times, proceed to the second subculture medium subculture once, proceed to the most again
One subculture medium continues propagation, according to needing Seedling amount repeat the above steps；
Wherein: the first subculture medium is 1/2MS+6-BA1.0mg/L+2,4-D
0.2mg/L+NAA 0.4mg/L+ agar 3g/L+ sucrose 15g/L；
Second subculture medium is 1/2MS+6-BA 2mg/L+NAA 0.1mg/L+ white sugar
35g/L+ agar powder 7.3g/L；
Condition of culture is: the PH6.5 of culture medium, and cultivation temperature is 25 ± 1 DEG C, the light of 16h/d
Cycle, about intensity of illumination 1200Lx.
5, root culture: the Herba Thymi through successive transfer culture is inoculated root media, and screening obtains
Obtain monomer plant.
Wherein, root media is: 1/2MS+IAA 0.2mg/L+ sucrose 30g/L+ agar 2g/L
+ quality of activated carbon ratio 0.2%；
Condition of culture is: the PH6.5 of culture medium, and cultivation temperature is 25 ± 1 DEG C, the light of 12h/d
Cycle, about intensity of illumination 1600Lx.
6, acclimatization and transplants: first culture bottle is moved to greenhouse, natural conditions lower refining seedling 4d, treats ground
When green pepper Seedling is healthy and strong and has 5-7 sheet true leaf, 6-8cm height, then untie sealed membrane, seedling exercising 6d, refining
Seedling takes out seedling after completing and cleans the culture medium of root attachment, is transplanted in sterilized substrate,
Temperature controls at 24-26 DEG C, and front 7d humid control, about 70%, is gradually lowered humidity later,
Until being placed under natural conditions by transplanted seedling, during acclimatization and transplants, suitable shading, makes intensity of illumination
For natural light 50%.
Embodiment 2: a kind of method of Herba Thymi fast breeding, including
Except, in subculture multiplication incubation, non-staining callus being proceeded to the first subculture training
Support in base and make Shoot propagation, after subculture 4 times, proceed to blank cultures subculture once, turn the most again
Entering and continue propagation in the first subculture medium, wherein blank cultures is 1/2MS+ biphosphate
Outside potassium 150mg/L+ lactoalbumin hydrolysate 500mg/L+ sucrose 30g/L, remaining is with embodiment 1.
Embodiment 3
Except " root media is: 1/2MS+IAA 0.1mg/L+ sucrose 20g/L+ agar
3g/L+ quality of activated carbon ratio 0.3% " outward, remaining is with embodiment 1.
The combination in any of any of the above condition, all can reach consistent effect.
The cultivation results of the present invention is: the inductivity of callus reaches 89%, and rooting rate reaches
85%, transplanting survival rate reaches 80%, and 10 generation aberration rates are below 6%.
Claims (2)
1. the method for a Herba Thymi fast breeding, it is characterized in that, including 1) take the flower pesticide disinfection of mid-late uninucleate stage, flower pesticide is immersed with sterile gauze parcel the 8-16s that sterilizes in the ethanol of 70-75% concentration, quickly remove to immerse immediately and bleaching powder soaks 10min, finally with aseptic water washing 4～6 times；2) inoculation callus inducing medium, cultivates and obtains callus；3) subculture multiplication is cultivated, proceed to non-staining callus the first subculture medium makes Shoot propagation, subculture 4 times, proceed to the second subculture medium or without subculture in the blank cultures of any hormone once, proceed to the first subculture medium continues propagation the most again, according to needing Seedling amount repeat the above steps；4) inoculation root media, screening obtains monomer plant；Described callus inducing medium is: 1/2MS+6-BA 1.2mg/L+NAA 0.12mg/L+ sucrose 50g/L；The pH6.6 of culture medium, cultivation temperature is 25 ± 1 DEG C, the photoperiod of 12h/d, intensity of illumination 1700Lx；The first described subculture medium is: 1/2MS+6-BA1.0mg/L+2,4-D 0.2mg/L+NAA 0.3～0.5mg/L+ agar 3g/L+ sucrose 15g/L；Described second subculture medium is: 1/2MS+6-BA 2mg/L+NAA 0.1mg/L+ white sugar 35g/L+ agar powder 7.3g/L；Described blank cultures is: 1/2MS+ potassium dihydrogen phosphate 150mg/L+ lactoalbumin hydrolysate 500mg/L+ sucrose 30g/L, and in Subculture, the pH6.5 of culture medium, cultivation temperature is 25 ± 1 DEG C, the photoperiod of 16h/d, intensity of illumination 1200Lx；Described root media is: 1/2MS+IAA 0.1～0.3mg/L+ sucrose 20～30g/L+ agar 2～3g/L+ quality of activated carbon ratio 0.1%～0.3%；Condition of culture is: the pH6.5 of culture medium, and cultivation temperature is 25 ± 1 DEG C, the photoperiod of 12h/d, intensity of illumination 1600Lx.
The method of Herba Thymi fast breeding the most according to claim 1, it is characterized in that, also include, acclimatization and transplants: first culture bottle is moved to greenhouse, natural conditions lower refining seedling 4d, treat that Herba Thymi Seedling is healthy and strong and has 5-7 sheet true leaf, during 6-8cm height, then sealed membrane is untied, seedling exercising 6d, seedling exercising takes out seedling after completing and cleans the culture medium of root attachment, it is transplanted in sterilized substrate, temperature controls at 24-26 DEG C, front 7d humid control is about 70%, it is gradually lowered humidity later, until transplanted seedling is placed under natural conditions, suitable shading during acclimatization and transplants, make intensity of illumination be nature light 50%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102388807A (en) * | 2011-10-27 | 2012-03-28 | 北京市园林学校 | Rapid propagation method for tissue culture of wild Thymus Mongolicus Ronn and application thereof |
| CN102550403A (en) * | 2011-11-04 | 2012-07-11 | 北京市园林学校 | Tissue culture rootage and transplantation methods for wild thymus mongolicus ronn |
| CN103858770A (en) * | 2014-04-03 | 2014-06-18 | 中国科学院植物研究所 | Rapid hosta plantagineu propagation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102388807A (en) * | 2011-10-27 | 2012-03-28 | 北京市园林学校 | Rapid propagation method for tissue culture of wild Thymus Mongolicus Ronn and application thereof |
| CN102550403A (en) * | 2011-11-04 | 2012-07-11 | 北京市园林学校 | Tissue culture rootage and transplantation methods for wild thymus mongolicus ronn |
| CN103858770A (en) * | 2014-04-03 | 2014-06-18 | 中国科学院植物研究所 | Rapid hosta plantagineu propagation method |
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| Title |
|---|
| 东北百里香组培再生体系的建立;王玲等;《园艺学报》;20111231;第38卷(第6期);第1186-1189页第1-2节 * |
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