CN104365469A - Anthocyanidin-rich health type wheat seed breeding method - Google Patents
Anthocyanidin-rich health type wheat seed breeding method Download PDFInfo
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- CN104365469A CN104365469A CN201410454993.XA CN201410454993A CN104365469A CN 104365469 A CN104365469 A CN 104365469A CN 201410454993 A CN201410454993 A CN 201410454993A CN 104365469 A CN104365469 A CN 104365469A
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Abstract
The present invention discloses an anthocyanidin-rich health type wheat seed breeding method, which comprises: 1, carrying out forced vernalization to achieve the height of 3 cm after wheat is subjected to dormancy-breaking germination-accelerating, 2, cutting the wheat at the tillering node position by adopting a sterile cutter blade, wherein the wheat tillering node upper portion is adopted as the scion, 3, drilling a grafting hole on anthocyanidin-rich heijingang potato; 4, inserting the scion into the grafting hole, 5, transferring into a nutrition incubator to culture for 30-35 days, spraying water, and carrying out foliar fertilization, 6, transferring into an intelligent climate chamber, and harvesting wheat seeds after 3 months, 7, carrying out graft backcrossing on 50% of the wheat seeds and the heijingang potato to obtain the second filial generation seed, and 8, carrying out third graft backcrossing on 50% of the second filial generation seed and the heijingang potatoes to obtain the third filial generation seed. According to the method of the present invention, the root cutting regeneration of the allopolyploid wheat is adopted to cultivate the new anthocyanidin-rich health type wheat variety so as to provide the anthocyanidin plant resource with characteristics of high yield, high content and high efficacy, wherein the anthocyanidin plant resource is used for processing of anthocyanidin food and beverages, and extraction of anthocyanidin.
Description
Technical field
The present invention relates to wheat breeding technical field, relate in particular to a kind of breeding method being rich in anthocyan health wheat.
Background technology
Anthocyan is a kind of strong antioxidant.In order to eliminate the damage of radical pair human body, the various diseases that prevention and therapy is relevant with free radical, as cancer, cardiopathy, hypertension, cross presenility, diabetes, arthritis etc., taking the health products that are rich in anthocyan or food becomes one of most effective method.The raw material extracting at present both at home and abroad anthocyan be generally purple grape, indigo plant suddenly, orange, tree coloured fruit, the crop and fruits such as purple sweetpotato, flower coloured in plant, coloured vegetables etc. such as Raphanus sativus var.niger, red pepper, tomato etc. are various such as to invite.But with these for raw material exists following shortcoming:
1, raw material planting and grown place limit by geographical conditions weather, and culture technique requires stricter;
2, pluck, preserve, transport inconvenience, and need stricter condition;
3, raw material output is low, and production cost is high.
Wheat is one of three large cereal, it is a kind of staple food crop of widely planting in the world, output is almost complete in edible, therefore, theoretically, by wheat and the plant hybridization containing anthocyan, selecting and be rich in anthocyan health new variety of wheat, thus provide the anthocyan plant resources of a kind of high yield, high-load, high effect, is a good research direction, but because wheat and the hybridization of the plant containing anthocyan belong to the edge vegetative hybridization far away across section, therefore successfully example is also few.
Summary of the invention
For solving above-mentioned the deficiencies in the prior art, the invention provides a kind of breeding method being rich in anthocyan health wheat, wheat is utilized to control the dominant gene of anthocyan, select and be rich in anthocyan health new variety of wheat, thus provide the anthocyan plant resources of a kind of high yield, high-load, high effect, for processing the extraction of anthocyan food, drink and anthocyan.
To achieve these goals, technical scheme of the present invention is:
Be rich in a breeding method for anthocyan health wheat, this breeding method comprises the steps:
1, the low temperature bud case that wheat puts into 0 ~ 3 degree after breaking dormancy vernalization carries out pressure vernalization, and complete the pressure vernalization of wheat after 35 ~ 40 days, now wheat is in seedling stage, high 3 centimeters;
2, wheat seedling is moved in calm aseptic intelligent biotron, in intelligence biotron, control temperature remains between 20 ~ 30 degree, humidity is 50 ~ 90%, with sterilization blade, wheat is cut off from tillering node, retain wheat tillering joint upper part and do scion, the cut-off part of wheat is sterilized;
3, do stock with the NCC potato being rich in anthocyan, going out grafting hole at NCC potato by the bit drills of 1.5mm, grafting hole depth is 5 ~ 15mm, to grafting hole and the sterilization of surrounding position;
4, scion is inserted in grafting hole, together tightly wrap as the NCC potato of stock and scion with medical proof fabric;
5, move in the nutrition incubator of sterilization treatment, plant in Nutrition Soil by the NCC potato of scion, Nutrition Soil is covered to more than grafting hole 5 ~ 10mm, nutrition incubator black out, and temperature is 20 ~ 25 degree, cultivates 30 ~ 35 days;
When just having entered nutrition incubator, carry out atomizing spray to the NCC potato of scion, spray in every 2 ~ 3 hours water, until when wheat grows first leaf, stops water spray;
The nutrient solution be made into nutrient, growth hormone and the basic element of cell division carries out foliar application, and each week sprays once, and spray concentration is 200ppm;
6, move into artificial intelligence climatic chamber, simulation wheat late growing stage phase condition, indoor temperature is 25 ~ 30 degree, humidity is 50 ~ 90%, daytime natural daylight, evening, after 3 months, wheat maturation, can gather in the crops wheat seed by simulated light 8 ~ 10 hours;
7, by wheat seed as first filial generation seed, 50% for field planting, therefrom obtains the plant of variation, and 50% as seed, again carries out grafting with NCC potato and backcrosses, and obtains second filial seed;
8, be used for field planting by 50% of second filial seed, therefrom obtain the plant of variation, 50% as seed, and third time carries out grafting with NCC potato and backcrosses, and obtains F3 seed.
As the improvement to technique scheme, in step 2 and step 3, the solution carried out disinfection to NCC potato, scion is absolute alcohol, and its concentration is 95%.
As the improvement to technique scheme, in steps of 5, described nutrient solution is nutrient, growth hormone and basic element of cell division Homogeneous phase mixing form, and its mass ratio is 1: 1: 1.
Compared with prior art, the beneficial effect acquired by the present invention is:
The present invention institute according to principle is, biological epigenetics is thought: any one cell with certain biological all hereditary information has the potential developing into intact organism, that is, each biological cell has totipotent feature, therefore, in theory, any one biological cell all has the potentiality developing into whole plant.The breeding method being rich in anthocyan health wheat of the present invention is analog resistant mutant, vegetative hybridization, gene order is not changed in breeding process, but gene expression there occurs can hereditary change, and this change is energy stable delivery in growth and cell proliferation process, thus obtain that descendant inheritting is stable is rich in anthocyan health wheat, this new variety of wheat is rich in anthocyan, thus provide the anthocyan plant resources of a kind of high yield, high-load, high effect, for processing the extraction of anthocyan food, drink and anthocyan.The new varieties that general breeding method needs 8-10 just can cultivate, and breeding method of the present invention shortens to triennium, just can cultivate new varieties, thus shorten breeding time, improve and cultivate new varieties speed.
Embodiment
Below in conjunction with specific embodiments of the invention, be clearly and completely described technical scheme of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1:
1, the low temperature bud case that wheat puts into more than 0 degree after breaking dormancy vernalization carries out pressure vernalization, and complete the pressure vernalization of wheat after 35 days, now wheat is in seedling stage, high 3 centimeters;
2, wheat seedling is moved in calm aseptic intelligent biotron, in intelligence biotron, control temperature remains on about 20 degree, humidity is 50%, with sterilization blade, wheat is cut off from tillering node, retain wheat tillering joint upper part and do scion, the cut-off part of wheat is sterilized with 95% absolute alcohol;
3, do stock with the NCC potato being rich in anthocyan, going out grafting hole at NCC potato by the bit drills of 1.5mm, grafting hole depth is 5mm, sterilizes to grafting hole and surrounding position 95% anhydrous essence of spilling;
4, scion is inserted in grafting hole, together tightly wrap as the NCC potato of stock and scion with medical proof fabric;
5, move in the nutrition incubator of sterilization treatment, plant in Nutrition Soil by the NCC potato of scion, Nutrition Soil is covered to the above 5mm in grafting hole, nutrition incubator black out, and temperature is 20 degree, cultivates 30 days;
When just having entered nutrition incubator, carry out atomizing spray to the NCC potato of scion, spray in an every 2 hours water, until when wheat grows first leaf, stops water spray;
The nutrient solution be made into nutrient, growth hormone and the basic element of cell division carries out foliar application, and each week sprays once, and spray concentration is 200ppm; Nutrient solution is nutrient, growth hormone and basic element of cell division Homogeneous phase mixing form, and its mass ratio is 1: 1: 1;
6, move into artificial intelligence climatic chamber, simulation wheat late growing stage phase condition, indoor temperature is 25 degree, humidity is 50%, daytime natural daylight, evening, after 3 months, wheat was ripe, can gather in the crops wheat seed by simulated light 8 hours;
7, by wheat seed as first filial generation seed, 50% for field planting, therefrom obtains the plant of variation, and 50% as seed, again carries out grafting with NCC potato and backcrosses, and obtains second filial seed;
8, be used for field planting by 50% of second filial seed, therefrom obtain the plant of variation, 50% as seed, and third time carries out grafting with NCC potato and backcrosses, and obtains F3 seed.
Embodiment 2:
1, the low temperature bud case that wheat puts into less than 3 degree after breaking dormancy vernalization carries out pressure vernalization, and complete the pressure vernalization of wheat after 40 days, now wheat is in seedling stage, high 3 centimeters;
2, wheat seedling is moved in calm aseptic intelligent biotron, in intelligence biotron, control temperature remains on about 30 degree, humidity is 90%, with sterilization blade, wheat is cut off from tillering node, retain wheat tillering joint upper part and do scion, the cut-off part of wheat is sterilized with 95% absolute alcohol;
3, do stock with the NCC potato being rich in anthocyan, going out grafting hole at NCC potato by the bit drills of 1.5 mm, grafting hole depth is 15mm, sterilizes to grafting hole and surrounding position 95% absolute alcohol;
4, scion is inserted in grafting hole, together tightly wrap as the NCC potato of stock and scion with medical proof fabric;
5, move in the nutrition incubator of sterilization treatment, plant in Nutrition Soil by the NCC potato of scion, Nutrition Soil is covered to the above 10mm in grafting hole, nutrition incubator black out, and temperature is 25 degree, cultivates 35 days;
When just having entered nutrition incubator, carry out atomizing spray to the NCC potato of scion, spray in an every 3 hours water, until when wheat grows first leaf, stops water spray;
The nutrient solution be made into nutrient, growth hormone and the basic element of cell division carries out foliar application, and each week sprays once, and spray concentration is 200ppm; Nutrient solution is nutrient, growth hormone and basic element of cell division Homogeneous phase mixing form, and its mass ratio is 1: 1: 1;
6, move into artificial intelligence climatic chamber, simulation wheat late growing stage phase condition, indoor temperature is 30 degree, humidity is 90%, daytime natural daylight, evening, after 3 months, wheat was ripe, can gather in the crops wheat seed by simulated light 10 hours;
7, by wheat seed as first filial generation seed, 50% for field planting, therefrom obtains the plant of variation, and 50% as seed, again carries out grafting with NCC potato and backcrosses, and obtains second filial seed;
8, be used for field planting by 50% of second filial seed, therefrom obtain the plant of variation, 50% as seed, and third time carries out grafting with NCC potato and backcrosses, and obtains F3 seed.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. be rich in a breeding method for anthocyan health wheat, it is characterized in that: this breeding method comprises the steps:
(1), the wheat low temperature bud case of putting into 0 ~ 3 degree after breaking dormancy vernalization carries out pressure vernalization, and complete the pressure vernalization of wheat after 35 ~ 40 days, now wheat is in seedling stage, high 3 centimeters;
(2), wheat seedling is moved in calm aseptic intelligent biotron, in intelligence biotron, control temperature remains between 20 ~ 30 degree, humidity is 50 ~ 90%, with sterilization blade, wheat is cut off from tillering node, retain wheat tillering joint upper part and do scion, the cut-off part of wheat is sterilized;
(3), with the NCC potato being rich in anthocyan do stock, going out grafting hole at NCC potato by the bit drills of 1.5mm, grafting hole depth is 5 ~ 15mm, to grafting hole and the sterilization of surrounding position;
(4), by scion insert in grafting hole, together tightly wrap as the NCC potato of stock and scion with medical proof fabric;
(5), move in the nutrition incubator of sterilization treatment, plant in Nutrition Soil by the NCC potato of scion, Nutrition Soil is covered to more than grafting hole 5 ~ 10mm, nutrition incubator black out, and temperature is 20 ~ 25 degree, cultivates 30 ~ 35 days;
When just having entered nutrition incubator, carry out atomizing spray to the NCC potato of scion, spray in every 2 ~ 3 hours water, until when wheat grows first leaf, stops water spray;
The nutrient solution be made into nutrient, growth hormone and the basic element of cell division carries out foliar application, and each week sprays once, and spray concentration is 200ppm;
(6), move into artificial intelligence climatic chamber, simulation wheat late growing stage phase condition, indoor temperature is 25 ~ 30 degree, humidity is 50 ~ 90%, daytime natural daylight, evening, after 3 months, wheat maturation, can gather in the crops wheat seed by simulated light 8 ~ 10 hours;
(7), by wheat seed as first filial generation seed, 50% for field planting, therefrom obtains the plant of variation, and 50% as seed, again carries out grafting with NCC potato and backcrosses, and obtains second filial seed;
(8), by 50% of second filial seed be used for field planting, therefrom obtain the plant of variation, 50% as seed, and third time carries out grafting with NCC potato and backcrosses, and obtains F3 seed.
2. the breeding method being rich in anthocyan health wheat according to claim 1, is characterized in that: in step 2 and step 3, and the solution carried out disinfection to NCC potato, scion is absolute alcohol, and its concentration is 95%.
3. the breeding method being rich in anthocyan health wheat according to claim 1 and 2, is characterized in that: in steps of 5, and described nutrient solution is nutrient, growth hormone and basic element of cell division Homogeneous phase mixing form, and its mass ratio is 1: 1: 1.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107018804A (en) * | 2017-05-31 | 2017-08-08 | 六枝特区家香农林种养殖农民专业合作社 | A kind of breeding method of tomato panax ginseng fruit |
| CN107581617A (en) * | 2016-07-09 | 2018-01-16 | 邵有林 | A kind of preparation method of the chromium-rich hypoglycemic fine agent of organic anthocyanidin selenium-rich |
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2014
- 2014-08-31 CN CN201410454993.XA patent/CN104365469A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107581617A (en) * | 2016-07-09 | 2018-01-16 | 邵有林 | A kind of preparation method of the chromium-rich hypoglycemic fine agent of organic anthocyanidin selenium-rich |
| CN107018804A (en) * | 2017-05-31 | 2017-08-08 | 六枝特区家香农林种养殖农民专业合作社 | A kind of breeding method of tomato panax ginseng fruit |
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Application publication date: 20150225 |