CN104360011B - A kind of method measuring U (VI) and U (IV) content in TGY nutrient culture media - Google Patents
A kind of method measuring U (VI) and U (IV) content in TGY nutrient culture media Download PDFInfo
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Abstract
The invention belongs to chemical analysis fields of measurement, be specially a kind of method measuring U (VI) and U (IV) content in TGY nutrient culture media.The method comprises the following steps: the typical curve of S1, making U (VI); S2, utilize U (VI) to prepare U (IV), in the HCl medium of 6mol/L, be reductive agent with zinc granule, prepare isocyatic U (IV); S3, containing in the TGY nutrient culture media of isocyatic U (VI) and U (IV), employing arsenazo Ⅲ metric measurement wherein U (VI) content; The red fuming nitric acid (RFNA) consumption that S4, selective oxidation U (IV) are total uranium etc. step.The method is by transferring the polymorphic analysis of U to single morphological analysis, namely spectrophotometric method is used to subtract U (VI) and total U in process measurement system by oxidation difference, deduct U (VI) by total U and draw U (IV), (IV) instability of U in nutrient culture media can be eliminated and microbiological culture media affects problem to it, convenient and swift.
Description
Technical field
The invention belongs to chemical analysis field of measuring technique, be specially a kind of method measuring U (VI) and U (IV) content in TGY nutrient culture media.
Background technology
Along with the development of nuclear industry, containing in the continuous entered environment of U radioactive waste, human health and ecologic environment are threatened.The average U concentration of crustal derm is 3mg/kg.From in the uranium-containing waste waters such as Uranium, nuclear power station, nuclear weapon experiment, the mass concentration of U is about 5mg/L, far above discharging standards (0.05mg/L).High concentration U can affect the normal function of kidney and bone in animal and human's body due to radioactivity and chemical toxicity.Therefore, the environment remediation problem that U pollutes has become current study hotspot.It is reported that the reparation of microorganism remediation U damage ratio physical chemistry has more significant advantage.
In physical environment, U has III, IV, V, VI tetra-kinds of valence states, and U (III), U (V) are unstable, and very easily disproportionation, namely U (VI) and U (IV) is two kinds of topmost valence state forms.Measurement because of U is subject to the impact of pH value in environment, organism, Redox Condition etc., and its chemical form can transform mutually in actual environment system, adds the difficulty of U Determination of Different Valence States.
At present, the measuring method of U (VI) and U (IV) is mainly arsenazo (III) spectrophotometric method, its principle is that U (VI) and U (IV) forms 1:1 complex compound with arsenazo (III) in acid condition, surveys its absorbance respectively in certain wave strong point.But due to containing a large amount of carbon source, nitrogenous source, inorganic salts etc. in microbiological culture media, these materials easily and effect such as U generation complexing, chelating etc., have an impact to the measurement of U.Arsenazo (III) spectrophotometric method is in the measuring process of U (VI) and U (IV), have ignored the instability of microbiological culture media on the impact of U and U (IV), and can not Measurement accuracy wherein U (IV) simultaneously.Therefore, in order to further investigate the redoxomorphism of microorganism to U, be necessary in microbiological culture media, set up the method that one can measure U (VI) and U (IV) simultaneously.
Summary of the invention
The object of the invention is to for above technical matters, improve a kind of by transferring the polymorphic analysis of U to single morphological analysis, namely spectrophotometric method is used to subtract U (VI) and total U in process measurement system by oxidation difference, deduct U (VI) by total U and draw U (IV), (IV) instability of U in nutrient culture media can be eliminated and microbiological culture media affects the measuring method of U (VI) and U (IV) in the microbiological culture media of problem to it.
The object of the invention is realized by following technical proposals:
A kind of method measuring U (VI) and U (IV) content in TGY nutrient culture media, comprises the following steps:
The typical curve of step S1, making U (VI);
Step S2, utilize prior art that U (VI) is prepared U (IV), Optimal reaction conditions is: in the HCl medium of 6mol/L, take zinc granule as reductive agent, be 10mg/L at U (VI) initial concentration, zinc granule concentration is 12g/L, reaction time is 180min, prepares and U (VI) isocyatic U (IV);
Step S3, get the TGY nutrient culture media of isocyatic U (VI) and U (IV), adopt arsenazo Ⅲ metric measurement wherein U (VI) content;
Drawn by both differences containing the content of U (IV) in the TGY nutrient culture media of U.U (IV) is calculated as follows:
C in formula
1-U (VI) starting point concentration, (mg/L); C
2-U (VI) residual concentration; (mg/L).
C in formula
(IV)---U (IV) concentration, (mg/L); C
total U---total U concentration, (mg/L); C
(VI)---U (VI) concentration, (mg/L).
The red fuming nitric acid (RFNA) consumption that step S4, selective oxidation U (IV) are total uranium, takes the red fuming nitric acid (RFNA) of different amounts to catch up with acid oxidase to the U (IV) in TGY nutrient culture media respectively; Red fuming nitric acid (RFNA) consumption is on the impact of U (VI) reduzate U (IV) oxygenation efficiency, and the oxygenation efficiency of U (IV) is calculated as follows:
C in formula
(VI)---U (VI) concentration, (mg/L); C
total U---total U concentration, (mg/L).
U (IV) is oxidized to U (VI) by step S5, employing red fuming nitric acid (RFNA), arsenazo Ⅲ developer and damping fluid is utilized to measure U (VI), i.e. total uranium content, containing utilizing total U to deduct U (VI) in U (VI) and U (IV) system thus indirect calculation can going out the content of U (IV).
The concrete steps of the typical curve of described making U (VI) are: U (VI) 2mL getting 1000mg/L, then 50mL is settled to deionized water, be mixed with the U (VI) of 40mg/L, to in the color comparison tube of 8 10mL, add 0mL, 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, 1.5mL, 2mL of 40mg/L respectively, and mass percentage is the arsenazo Ⅲ developer 1mL of 0.05%, and adopt damping fluid to be settled to 10mL, fully shake up, leave standstill 30min, measure absorbance in 652nm place.
The concrete steps of arsenazo Ⅲ metric measurement U (VI) are adopted to be in step S3: i.e. 2mL sample, adding 1mL mass percentage is 0.05% arsenazo Ⅲ nitrite ion, and adopt 0.4mol/L chloroacetic acid-0.4mol/L sodium chloroacetate damping fluid to be settled to 10mL, leave standstill 30min, measure its absorbance at wavelength 652nm place.
The concrete steps of the content of the total U quantitative determination of the utilization described in step S4 U (VI) are: get 2mL sample, employing 1mL mass percentage is that acid is caught up with in the red fuming nitric acid (RFNA) heating of 65%-68%, keep slight fluidized state, until there is White residule, carry out dissolving and being settled to 5mL with deionized water, with the content of arsenazo Ⅲ spectrophotometric method test U (VI), this is that total U measures, and is drawn by both differences containing the content of U (IV) in U mixed system.
Sample described in step S3 and step S4 is the TGY nutrient culture media of i.e. isocyatic U (VI) and U (IV).
Good effect of the present invention is embodied in:
Measuring method is simple and quick, can eliminate in TGY nutrient culture media that U (IV) instability and microbiological culture media are on the problem of the impact that it is measured, and accuracy rate is high.
Accompanying drawing illustrates:
Fig. 1 is the canonical plotting of U (VI);
Fig. 2 is the influence curve figure that U (VI) concentration is prepared U (IV);
Fig. 3 is the influence curve figure that zinc granule concentration is prepared U (IV);
Fig. 4 is the influence curve figure prepared U (IV) in the reaction time
Fig. 5 is the influence curve figure of nitric acid dosage to U (IV) oxygenation efficiency
Embodiment
Arbitrary feature disclosed in this instructions (comprising any accessory claim, summary), unless specifically stated otherwise, all can be replaced by other equivalences or the alternative features with similar object.That is, unless specifically stated otherwise, each feature is an example in a series of equivalence or similar characteristics.
U (IV) all in the application all represents uranous, and U (VI) all represents hexavalent uranium.
Embodiment 1:
The material that the present embodiment adopts and reagent as follows:
TGY nutrient culture media (tryptone yeast dextrose culture-medium): tryptone 5g, dusty yeast 3g, glucose 1g, is settled to 1000mL, and pH value is 7.0-7.2.
1000mg/LU (VI) standard reserving solution: 1.7822g acetic acid dioxygen U (UO
2(CH
3cOO)
2h
2pu Zhen bio tech ltd, O Shanghai), dissolve and be settled to 1000mL, shaking up, keeping in Dark Place, other each concentration U (VI) thus solution dilution obtain.
0.05% arsenazo (III) nitrite ion (Shanghai Aladdin): arsenazo (III) 0.05g, is settled to 100mL.
Arsenic-free zinc granule (Chengdu Ke Long chemical reagent factory).It is pure that other reagent are domestic analysis.
The making of U (VI) typical curve
Get 1000mg/LU (VI) 2mL, deionized water is settled to 50mL, be mixed with 40mg/LU (VI), 8 10mL color comparison tubes, add respectively 40mg/LU (VI) 0,0.2,0.4,0.6,0.8,1.0,1.5,2mL, 0.05% arsenazo Ⅲ 1mL, damping fluid is settled to 10mL, fully shake up, leave standstill 30min, survey its absorbance in 652nm place.
With U (VI) concentration for horizontal ordinate, absorbance is ordinate, U (VI) typical curve is obtained after matching, result as shown in Figure 1, as shown in Figure 1, typical curve equation y=0.040x-0.007, R2=0.999, within the scope of ρ (U)=0.0-20.0mg/L, absorbance ~ ρ (U) relation character closes Beer law.
The preparation method of U (IV)
Under arsenazo (III) color condition, first measure U (VI) in mixed system, become U (VI) with concentrated nitric acid oxidation U (IV) again, measure total U thus can indirect inspection U (IV), make multicomponent analysis convert single component analysis to.Arsenazo (III) spectrophotometric method test U (VI), i.e. 2mL sample, 1mL mass percentage is 0.05% arsenazo (III) nitrite ion, 0.4mol/L chloroacetic acid-0.4mol/L sodium chloroacetate damping fluid is settled to 10mL, leave standstill 30min, measure its absorbance at wavelength 652nm place; Arsenazo (III) spectrophotometric method test U (IV) namely first tests U (VI) content in sample, separately getting 2mL sample adopts the heating of 1mL red fuming nitric acid (RFNA) to catch up with acid, keep slight boiling condition, until there is White residule, dissolve White residule and be settled to 5mL.With arsenazo (III) spectrophotometric method test U (VI) content, this is that total U measures.Drawn by both differences containing the content of U (IV) in U mixed system.U (IV) is calculated as follows:
C in formula
1-U (VI) starting point concentration, (mg/L); C
2-U (VI) residual concentration; (mg/L).
C in formula
(IV)---U (IV) concentration, (mg/L); C
total U---total U concentration, (mg/L); C
(VI)---U (VI) concentration, (mg/L).
The mass percentage of red fuming nitric acid (RFNA) is 65%-68%, because red fuming nitric acid (RFNA) is strong oxidizer, namely uses it to be oxidized U (VI) reduzate U (IV).Its oxidation mechanisms be red fuming nitric acid (RFNA) when acidity is higher, be easily decomposed to form nitrous acid and oxides of nitrogen, nitrous acid character is active, and be easy to U (IV) to be oxidized to U (VI), reaction equation is:
U
4++2HNO
2→UO
2 2++2NO+2H
+
Spectrophotometric method preci-sion and accuracy is analyzed
The continuous mixed solution measuring 5mg/L equal-volume U (VI) and U (IV) for 5 times, substitutes into U (VI) standard working curve.Investigate the preci-sion and accuracy of U (VI) and U (IV).
Embodiment 2:
Adopt the method in embodiment 1 and step, measure the impact that initial U (VI) concentration is prepared U (IV).
Get respectively 1000mg/LU (VI) 0.2,0.3,0.4,0.5,0.6,0.7,0.8,0.9,1.0mL in 50mL volumetric flask, add 6mol/LHCl45mL, add zinc granule 0.6g, make its concentration be 12g/L, constant volume.Its final concentration is respectively 4,6,8,10,12,14,16,18, the U (VI) of 20mg/L.
Keep U (VI) final concentration in reaction system to be respectively 4,6,8,10,12,14,16,18,20mg/L, HCl concentration 6mol/L, zinc granule concentration 12g/L, total reaction volume is 50mL.U (IV) production rate is shown in Fig. 2 with the change of U (VI) initial concentration.As shown in Figure 2, within the scope of 4-10mg/LU (VI) final concentration, U (IV) production rate keeps steady state (SS) along with the increase of U (VI) initial concentration, reaches about 95%.But within the scope of 10-20mg/LU (VI) final concentration, U (IV) production rate is along with the increase of U (VI) initial concentration, and production rate sharply declines.Therefore, the suitableeest U (VI) initial concentration is selected to be final concentration 10mg/LU (VI) in the application.
Embodiment 3:
Adopt the method in embodiment 1 and step, measure the impact that zinc granule grain concentration is prepared U (IV)
Get 1000mg/LU (VI) 0.5mL and, in 50mL volumetric flask, add 6mol/LHCl45mL, add zinc granule amount be respectively 0.2,0.4,0.6,0.8,1.0,1.2,1.4,1.6,1.8,2.0g, constant volume.Make zinc granule concentration be respectively 4,8,12,16,20,24,28,32,36g/L.
To keep U (VI) final concentration in reaction system to be 10mg/L, HCl concentration is 6mol/L, total reaction volume is 50mL, the zinc granule concentration in reaction system is respectively 4,8,12,16,20,24,28,32,36g/L.U (IV) production rate is shown in Fig. 3 with the change of zinc granule concentration.As shown in Figure 3, zinc granule concentration is within the scope of 4-12g/L, and U (IV) production rate increases along with the increase of zinc granule concentration.When zinc granule concentration is 12g/L, production rate reaches maximum.But when zinc granule concentration is greater than 28g/L, production rate in significantly reducing trend, and has typical black flocculent deposit.When inferring that zinc granule concentration is excessive, considerable influence can be had to the measurement of U (VI).Therefore the suitableeest zinc granule concentration is selected to be 12g/L in the application.
Embodiment 4:
Adopt the method in embodiment 1 and step, reaction time impact prepared by U (IV) in determination step S2
Get 1000mg/LU (VI) 0.5mL and, in 50mL volumetric flask, add 6mol/LHCl45mL, add zinc granule and make its concentration be 12g/L, the reaction time is respectively 90,120,150,180,210min, constant volume.
U (VI) final concentration in maintenance reaction system is 10mg/L, HCl concentration is 6mol/L, total reaction volume is 50mL, and zinc granule concentration 12g/L, the reaction time is respectively 90-210min.U (IV) production rate is shown in Fig. 4 with the change in reaction time.As shown in Figure 4, U (IV) production rate increases a little to some extent along with the increase in reaction time, when being 180min when reacted, then increases the reaction time, and the change of U (IV) production rate is not obvious.Therefore, the optimal reaction time of selecting in the application is 180min.
Draw by embodiment 2-embodiment 4 and from Fig. 2-Fig. 4, U (VI) initial concentration, zinc granule concentration, reaction time affect 3 key factors prepared by U (IV).From result of study, U (VI) final concentration is 10mg/L, zinc granule concentration is 12g/L, reaction time 180min is optimal conditions prepared by U (IV), and the production rate of U (IV) reaches about 95%.
Embodiment 5:
Adopt the method in embodiment 1 and step, measure red fuming nitric acid (RFNA) consumption subtracts process impact on U (VI) reduzate U (IV) oxidation difference.
Get final concentration be 10mg/LU (VI) reduzate U (IV) 25mL in 50mL volumetric flask, TGY nutrient culture media constant volume.Add 0 respectively, 0.5,1.0,1.5,2.0,2.5,3.0mL red fuming nitric acid (RFNA) is oxidized the U (IV) in 2mL nutrient culture media, reacted rear arsenazo (III) metric measurement U (VI), i.e. total U.Red fuming nitric acid (RFNA) consumption is on the impact of U (VI) reduzate U (IV) oxygenation efficiency, and the oxygenation efficiency of U (IV) is calculated as follows:
C in formula
(VI)---U (VI) concentration, (mg/L); C
total U---total U concentration, (mg/L).
Be in the reaction system of 5mg/LU (VI) reaction product U (IV) at final concentration, sampling liquid 2mL, add 0 respectively, 0.5,1.0,1.5,2.0,2.5,3.0mL nitric acid is oxidized U (IV), use the total U of arsenazo (III) metric measurement, combined standard curve calculating U (VI) content, i.e. total U content.U (IV) oxygenation efficiency is shown in Fig. 5 with the change of red fuming nitric acid (RFNA) consumption.As shown in Figure 5, red fuming nitric acid (RFNA) consumption is comparatively large on U (IV) oxygenation efficiency impact in TGY nutrient culture media, nitric acid dosage within the scope of 0-1mL, along with the increase oxygenation efficiency of nitric acid dosage increases; When nitric acid dosage is within the scope of 1-2mL, oxygenation efficiency can reach about 98%; But it is on a declining curve subsequently.Micro-nitric acid residual in supposition system can change the acidity of system, thus have impact on composition and the stability of U (VI) and developer arsenazo (III), namely have impact on the measurement of U (VI).Therefore, red fuming nitric acid (RFNA) consumption is selected to be 1mL.And with Liu Jinwei etc., this research points out that geological sample is through HF-HNO
3-HCl-HClO
4after nitrated, organic unanimously noiseless to the measurement of U in sample, namely illustrate that in spectrophotometric method, oxidation difference subtracts the impact that process can eliminate the macromolecular complex such as amino acid, organic matter confrontation U measurement in nutrient culture media.
Spectrophotometric method preci-sion and accuracy is analyzed
In the TGY nutrient culture media of equal-volume mixing 5mg/LU (VI) and 5mg/LU (IV), use arsenazo (III) spectrophotometric method combination oxidation difference to subtract process parallel and measure 5 times, carry out preci-sion and accuracy analysis, result is shown in table 1.
The preci-sion and accuracy of table 1 method
Table1Precisionandaccuracyanalysisofmethod
From table 1 and U (VI) typical curve, the range of linearity that U (VI) measures is at 0.0-20.0mg/L, and lower limit can reach 0.05mg/L, and the recovery is 96%, RSD=1.7%, and error is-4%.Therefore, this method can be used for the measurement of U in biological sample (VI) and U (IV).
Claims (6)
1. measure a method for U (VI) and U (IV) content in TGY nutrient culture media, it is characterized in that comprising the following steps:
The typical curve of step S1, making U (VI);
Step S2, utilize U (VI) to prepare U (IV), in the HCl medium of 6mol/L, take zinc granule as reductive agent, U (VI) initial concentration is 10mg/L, prepares and U (VI) isocyatic U (IV);
Step S3, get isocyatic U (VI) and U (IV) mixed culture medium, adopt the content of arsenazo Ⅲ metric measurement wherein U (VI);
The red fuming nitric acid (RFNA) consumption that step S4, selective oxidation U (IV) are total uranium, takes different amounts red fuming nitric acid (RFNA) to catch up with acid oxidase to the U (IV) in TGY nutrient culture media respectively;
U (IV) is oxidized to U (VI) by step S5, employing red fuming nitric acid (RFNA), utilize arsenazo Ⅲ chromogenic reagent and 0.4mol/L chloroacetic acid-0.4mol/L sodium chloroacetate damping fluid constant volume, measure U (VI), i.e. total uranium content, containing in the mixed system of U (VI) and U (IV), utilize total U amount to deduct in step S3 and measure U (VI) thus the content calculating U (IV).
2. the method measuring U (VI) and U (IV) content in TGY nutrient culture media according to claim 1, it is characterized in that the concrete steps of the typical curve of the making U (VI) described in step S1 are: U (VI) 2mL getting 1000mg/L, then 50mL is settled to deionized water, be mixed with the U (VI) of 40mg/L, to in the color comparison tube of 8 10mL, add U (VI) 0mL of 40mg/L respectively, 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, 1.5mL, 2mL, and mass percentage is the arsenazo Ⅲ developer 1mL of 0.05%, and adopt damping fluid to be settled to 10mL, fully shake up, leave standstill 30min, absorbance is measured in 652nm place.
3. the method measuring U (VI) and U (IV) content in TGY nutrient culture media according to claim 1, it is characterized in that in described step S2, utilizing U (VI) to prepare U (IV), its reaction conditions is: in the HCl medium of 6mol/L, take zinc granule as reductive agent, U (VI) initial concentration is 10mg/L, zinc granule concentration is 12g/L, and the reaction time is 180min, prepares and U (VI) isocyatic U (IV).
4. the method measuring U (VI) and U (IV) content in TGY nutrient culture media according to claim 1, it is characterized in that adopting in described step S3 the concrete steps of arsenazo Ⅲ metric measurement U (VI) be: to get 2mL sample, i.e. isocyatic U (VI) and U (IV) mixed culture medium, adding 1mL mass percentage is 0.05% arsenazo Ⅲ nitrite ion, and adopt 0.4mol/L chloroacetic acid-0.4mol/L sodium chloroacetate damping fluid to be settled to 10mL, leave standstill 30min, measure its absorbance at wavelength 652nm place.
5. the method measuring U (VI) and U (IV) content in TGY nutrient culture media according to claim 1, it is characterized in that utilization total U quantitative determination U (VI) described in described step S5 thus the concrete steps calculating the content of U (IV) are: get 2mL sample, namely containing the TGY nutrient culture media of isoconcentration U (VI) and U (IV), the heating of 1mL red fuming nitric acid (RFNA) is adopted to catch up with acid, it is made to keep slight boiling condition with heating by electric cooker at normal temperatures, until there is White residule, be settled to 5mL with deionized water dissolving White residule, with the content of arsenazo Ⅲ spectrophotometric method test U (VI), this is that total U measures, containing in U (VI) and U (IV) system, U (VI) content was measured and measured in step S3 to the content of U (IV) difference by total U draws.
6. the method measuring U (VI) and U (IV) content in TGY nutrient culture media according to claim 1, is characterized in that the mass percentage of the red fuming nitric acid (RFNA) described in step S5 is 65%-68%.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102156105A (en) * | 2011-03-31 | 2011-08-17 | 东华理工大学 | Site uranium fast analysis method and special reagent packet for method |
| CN102211017A (en) * | 2011-06-11 | 2011-10-12 | 中国海洋大学 | Amidoxime group uranium extraction sorbent and preparation method thereof |
| CN103954596A (en) * | 2014-04-18 | 2014-07-30 | 广西大学 | Method for indirectly measuring content of uranium in soil through atomic fluorescence spectroscopy |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102156105A (en) * | 2011-03-31 | 2011-08-17 | 东华理工大学 | Site uranium fast analysis method and special reagent packet for method |
| CN102211017A (en) * | 2011-06-11 | 2011-10-12 | 中国海洋大学 | Amidoxime group uranium extraction sorbent and preparation method thereof |
| CN103954596A (en) * | 2014-04-18 | 2014-07-30 | 广西大学 | Method for indirectly measuring content of uranium in soil through atomic fluorescence spectroscopy |
Non-Patent Citations (1)
| Title |
|---|
| CL-TBP萃淋树脂分离分光光度法连续测定微量铀和钍;周京霞 等;《湿法冶金》;20011231;第20卷(第4期);全文 * |
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