CN104328147A - Production method of chlorine-contaning (2R,3S) methyl methylpropionate - Google Patents
Production method of chlorine-contaning (2R,3S) methyl methylpropionate Download PDFInfo
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- CN104328147A CN104328147A CN201410518181.7A CN201410518181A CN104328147A CN 104328147 A CN104328147 A CN 104328147A CN 201410518181 A CN201410518181 A CN 201410518181A CN 104328147 A CN104328147 A CN 104328147A
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- 238000004519 manufacturing process Methods 0.000 title description 2
- BHIWKHZACMWKOJ-UHFFFAOYSA-N methyl isobutyrate Chemical compound COC(=O)C(C)C BHIWKHZACMWKOJ-UHFFFAOYSA-N 0.000 title description 2
- 239000000758 substrate Substances 0.000 claims abstract description 70
- 229920000742 Cotton Polymers 0.000 claims abstract description 62
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 20
- 210000004027 cell Anatomy 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 17
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 141
- 239000000243 solution Substances 0.000 claims description 46
- 238000006243 chemical reaction Methods 0.000 claims description 35
- 239000007788 liquid Substances 0.000 claims description 18
- 238000000605 extraction Methods 0.000 claims description 17
- 238000009423 ventilation Methods 0.000 claims description 17
- 235000015097 nutrients Nutrition 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 13
- 239000008103 glucose Substances 0.000 claims description 13
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 12
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 12
- 244000068988 Glycine max Species 0.000 claims description 12
- 235000010469 Glycine max Nutrition 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 208000012839 conversion disease Diseases 0.000 claims description 7
- 229960003487 xylose Drugs 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 6
- 238000003810 ethyl acetate extraction Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 229940075507 glyceryl monostearate Drugs 0.000 claims description 6
- 239000013028 medium composition Substances 0.000 claims description 6
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 claims description 6
- 239000002953 phosphate buffered saline Substances 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 6
- 235000019698 starch Nutrition 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 239000007858 starting material Substances 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 230000009467 reduction Effects 0.000 claims description 4
- 238000006722 reduction reaction Methods 0.000 abstract description 5
- 230000002829 reductive effect Effects 0.000 abstract description 4
- 239000003054 catalyst Substances 0.000 abstract description 2
- 241001661345 Moesziomyces antarcticus Species 0.000 abstract 1
- 239000004744 fabric Substances 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 229940017219 methyl propionate Drugs 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 abstract 1
- 229920002301 cellulose acetate Polymers 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000010531 catalytic reduction reaction Methods 0.000 description 4
- 150000002148 esters Chemical class 0.000 description 4
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 239000005515 coenzyme Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 230000002210 biocatalytic effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000012847 fine chemical Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 241000024287 Areas Species 0.000 description 1
- 240000008564 Boehmeria nivea Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 101710157860 Oxydoreductase Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 238000011914 asymmetric synthesis Methods 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 150000001728 carbonyl compounds Chemical class 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
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- General Engineering & Computer Science (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
In the invention, with a candida antarctica cell as a catalyst, chiral (-)-(2R,3S)-3-hydroxyl-3-(4-chlorophenyl)-2-methyl methylpropionate is prepared through a biological reduction reaction. A reactor is filled with a phosphate buffer solution. A cotton yarn cloth is added for adsorbing a substrate and a product for adjusting concentrations of the substrate and the product so that inhibition on cells can be effectively reduced. By means of the yeast cell for carrying out the biological reduction reaction, the yield of the product is high and an enantiomeric excess ratio is high. The method is quite good in application value.
Description
Technical field
The invention belongs to technology of pharmaceutical engineering field, be related specifically to the reduction of antarctic candida cell biological and prepare chiral medicinal intermediate ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-) technology of-2 Methylpropionic acid methyl esters.
Background technology
Hydroxyl chiral ester is the important intermediate of synthesis of chiral medicine, fine chemicals, agricultural chemicals product and other exotic materialss.The asymmetric reduction of biocatalysis carbonyl compound has environmental friendliness, mild condition, selectivity advantages of higher, prepares the first approach of hydroxyl chiral ester as green high-efficient, is applied to the hydroxyl chiral ester producing some high added values more and more.Biocatalysis is prepared hydroxyl chiral ester and is had good application prospect.
Biocatalysis has the outstanding advantages such as catalytic efficiency is high, selectivity strong, mild condition, environmental friendliness, is the important method substituting and expand traditional organic chemical synthesis in process of sustainable development.Wherein chiral separation and asymmetric synthesis are the Application Areass of biocatalysis most magnetism.As in six large fermentoids of biological catalyst, lytic enzyme catalytic kinetics resolution of racemates can obtain quiral products, in industrial biocatalytic, play key player always.In recent years, oxydo-reductase application industrially obtained and increased rapidly.At present, the ratio adopting the industry of lytic enzyme Kinetic Resolution, biological reducing method and biological oxidation process to prepare optical activity chirality compound is 4:2:1.
Biological reducing method is for Kinetic Resolution, and maximum advantage is that theoretical yield can reach 100%, and Atom economy is good.But bioreduction needs the participation of coenzyme or cofactor, limit its application to a certain extent.Due to the dependent cause of coenzyme, in bioreduction, many Bian intact cells are as catalyzer, realize the purification procedures eliminating enzyme in body while coenzyme cyclic regeneration.
()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters is the important chiral building block of synthesis of chiral medicine, fine chemicals, agricultural chemicals product, owing to there being 2 chiral centres, make chemosynthesis comparatively difficult, cost is high.The present invention will adopt biocatalysis to prepare ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters.
Summary of the invention
The present invention adopts antarctic candida cell catalysis to prepare ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters, reaction formula is as follows:
Substrate 2-(4-chlorobenzoyl)-2-methyl acrylate (1) through antarctic candida catalytic reduction, obtain product ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters (2).There is multiple-microorganism can catalytic reduction, through great many of experiments screening, finally determine to adopt yeast as catalyzer, because its catalytic reduction
1effect best, reaction yield, enantiomeric excess rate (ee%) are all very high.
Many antarctic candidas can carry out biocatalytic reduction, but its effect is different, differs greatly, and through experiment, the present invention selects antarctic candida bacterial strain to be
aTCC 28323, its catalytic reduction best results.
developing medium:
1, nutrient solution composition: glucose 18-20 g/L, starch 13-15g/L, analysis for soybean powder 28-29 g/L, yeast extract 6-7 g/L, tween 80 0.6-0.8 g/L, KH
2pO
42-2.4 g/L, MgSO
4.7H
2o 0.3-0.4 g/L, soya-bean oil 20-25 mL/L, malt extract 26-30 g/L.
2, solid medium composition: add the agar powder of 2% in liquid medium within.
Prepared by yeast cell.Antarctic candida through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, coefficient is 0.6-0.7,121 DEG C of autoclavings 30 minutes, be cooled to 24-25 DEG C, yeast starter liquid is seeded to fermentor tank, inoculative proportion is 9-10%, ventilation ratio is 1-1.3V/(V minute), cultivate 49-54 hour, obtain wet yeast cell, as bioreduction catalyzer with filtering centrifuge is centrifugal for 24-25 DEG C.
Because substrate, product all have restraining effect to yeast cell, in order to reduce the suppression of substrate, products upon cell, the present invention adopts cotton gauze immunoabsorbent substrate, in reaction, when substrate is reduced by cell catalysis, when concentration reduces, substrate from cotton gauze stripping, postreaction consume substrate.Meanwhile, product is adsorbed by cotton gauze, decreases its concentration in water, thus considerably reduces the suppression of substrate, products upon cell.The ratio of substrate and cotton gauze, determines the concentration of substrate in reaction solution, product, and concentration is also relevant with temperature of reaction, reaction solution composition.Different cell is different to the susceptibility of substrate, product, so, great many of experiments be carried out, best substrate and the ratio of cotton gauze could be determined.Experiment shows, for bacterial strain of the present invention, reaction solution composition, temperature of reaction, best substrate and the ratio of cotton gauze are 0.3-0.32.During concrete absorption, substrate is dissolved in ethyl acetate, the ratio of substrate and ethyl acetate is 0.5:2, obtain the ethyl acetate solution of substrate, absorb this solution with the cotton gauze being of a size of 0.5cmX0.5cm, then, dry, reclaim ethyl acetate, namely obtain the cotton gauze of immunoabsorbent substrate.Regulate substrate and cotton gauze consumption, make the quality ratio of substrate and cotton gauze be 0.3-0.32.Cotton gauze used is common cotton gauze, and commercially, be cut into 0.5cmX0.5cm fritter, sterilizing, Preservation in sterile condition is stand-by.
Phosphate buffered saline buffer is added in bottom ventilation stirred tank, coefficient is 0.6-0.7, pH is 6.3, add the cotton gauze of immunoabsorbent substrate, making substrate 2-(4-chlorobenzoyl)-2-methyl acrylate addition is 80-90 g/L, glucose content is 8-9g/L, wood sugar 6-7 g/L, glyceryl monostearate content is 12-15g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 24-25 DEG C, add wet yeast cell and make concentration be 22-25g/L, ventilation ratio is 0.10-0.12V/(V minute), namely per minute air flow is 0.1-0.12 times of reaction solution volume, carries out bioreduction, and the reaction times is 80-85 hour; After reaction terminates, first leach cell, leach cotton gauze again, with the extraction into ethyl acetate reaction solution 2 times of 0.3 times of reaction volume, with the extraction into ethyl acetate cotton gauze 3 times of 3 times of cotton gauze quality, the ethyl acetate of combined ethyl acetate extraction liquid and extraction gauze, steam ethyl acetate, obtain product ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters, reaction conversion ratio 94-96%, product yield 92-94%, enantiomeric excess rate (ee%) 97-98%.
The present invention carries out the work and comprises bacterial strain selection (selecting from more or less a hundred bacterial strain), catalytic reductive conditions optimizes (temperature of reaction, air flow, pH), reaction medium is selected and concentration optimization (concentration of substrate, glucose content, Xylose Content, kinds of surfactants (more than 20, kind selects 1) and concentration, other multiple components is selected to get rid of), solid absorption is adopted to control substrate product concentration, thus the suppression reduced cell, test carclazyte, diatomite, cotton gauze, ramie gauze, the many kinds of solids materials such as macropore resin, also once carried out the test of water-organic solvent 2 phase system.Because tested number is very large, although have employed response surface optimization design experiment, drastically reduce the area tested number, tested number is still very large, and total Test carries out just completing more than 2 years, reaches current technical scheme.For photolytic activity product, as reaction conversion ratio 94-96%, during product yield 92-94%, enantiomeric excess rate (ee%), still up to 97-98%, is very not easily, and our work achieves marked improvement.
embodiment 1
Nutrient solution nutrient solution composition is: glucose 18-20 g/L, starch 13-15g/L, analysis for soybean powder 28-29 g/L, yeast extract 6-7 g/L, tween 80 0.8 g/L, KH
2pO
42.2g/L, MgSO
4.7H
2o 0.3 g/L, soya-bean oil 20 mL/L, malt extract 26 g/L.Solid medium composition: add the agar powder of 2% in liquid medium within.
Wet yeast cell preparation process is as follows: antarctic candida through inclined-plane, shaking flask, seed tank culture obtain seed liquor; 10L fermentor tank adds nutrient solution, coefficient is 0.6,121 DEG C of autoclavings 30 minutes, are cooled to 24 DEG C, yeast starter liquid are seeded to fermentor tank, inoculative proportion is 9%, ventilation ratio is 1V/(V minute), namely per minute air flow is 1 times of reaction solution volume, cultivates 49 hours for 24 DEG C, wet yeast cell is obtained, as bioreduction catalyzer with filtering centrifuge is centrifugal.
The cotton gauze making method of immunoabsorbent substrate is, substrate is dissolved in ethyl acetate, the ratio of substrate and ethyl acetate is 0.5:2, obtain the ethyl acetate solution of substrate, absorb this solution with the cotton gauze being of a size of 0.5cmX0.5cm, then, dry, reclaim ethyl acetate, namely obtain the cotton gauze of immunoabsorbent substrate, regulate substrate and cotton gauze consumption, make the quality ratio of substrate and cotton gauze be 0.3.Cotton gauze used is common cotton gauze, and commercially, be cut into 0.5cmX0.5cm fritter, sterilizing, Preservation in sterile condition is stand-by, and other embodiment cotton gauze process is identical.
Phosphate buffered saline buffer is added in 15L bottom ventilation stirred tank, coefficient is 0.6, pH is 6.3, add the cotton gauze of immunoabsorbent substrate, making substrate 2-(4-chlorobenzoyl)-2-methyl acrylate addition is 80 g/L, glucose content is 8g/L, wood sugar 6 g/L, glyceryl monostearate content is 12g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 24 DEG C, add wet yeast cell and make concentration be 22g/L, ventilation ratio is 0.10V/(V minute), namely per minute air flow is 0.1 times of reaction solution volume, carries out bioreduction, and the reaction times is 80 hours; After reaction terminates, first leach cell, then leach cotton gauze, with the extraction into ethyl acetate reaction solution 2 times of 0.3 times of reaction volume, with the extraction into ethyl acetate cotton gauze 3 times of 3 times of cotton gauze quality, the ethyl acetate of combined ethyl acetate extraction liquid and extraction gauze, steams ethyl acetate, obtains product ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters, reaction conversion ratio 94%, product yield 92%, enantiomeric excess rate (ee%) 98%.
embodiment 2
Nutrient solution nutrient solution composition is: glucose 20 g/L, starch 15g/L, analysis for soybean powder 29 g/L, yeast extract 7 g/L, tween 80 0.6 g/L, KH
2pO
42.4 g/L, MgSO
4.7H
2o 0.4 g/L, soya-bean oil 20 mL/L, malt extract 30 g/L.Solid medium composition: add the agar powder of 2% in liquid medium within.
Wet yeast cell preparation process is as follows: antarctic candida through inclined-plane, shaking flask, seed tank culture obtain seed liquor; 50L fermentor tank adds nutrient solution, coefficient is 0.7,121 DEG C of autoclavings 30 minutes, are cooled to 25 DEG C, yeast starter liquid are seeded to fermentor tank, inoculative proportion is 10%, ventilation ratio is 1.3V/(V minute), namely per minute air flow is 1.3 times of reaction solution volumes, cultivates 54 hours for 25 DEG C, wet yeast cell is obtained, as bioreduction catalyzer with filtering centrifuge is centrifugal.
The cotton gauze making method of immunoabsorbent substrate is, substrate is dissolved in ethyl acetate, the ratio of substrate and ethyl acetate is 0.5:2, obtain the ethyl acetate solution of substrate, absorb this solution with the cotton gauze being of a size of 0.5cmX0.5cm, then, dry, reclaim ethyl acetate, namely obtain the cotton gauze of immunoabsorbent substrate, regulate substrate and cotton gauze consumption, make the quality ratio of substrate and cotton gauze be 0.32.Cotton gauze used is common cotton gauze, and commercially, be cut into 0.5cmX0.5cm fritter, sterilizing, Preservation in sterile condition is stand-by, and other embodiment cotton gauze process is identical.
Phosphate buffered saline buffer is added in 100L bottom ventilation stirred tank, coefficient is 0.7, pH is 6.3, add the cotton gauze of immunoabsorbent substrate, making substrate 2-(4-chlorobenzoyl)-2-methyl acrylate addition is 90 g/L, glucose content is 8-9g/L, wood sugar 7 g/L, glyceryl monostearate content is 15g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 25 DEG C, add wet yeast cell and make concentration be 25g/L, ventilation ratio is 0.12V/(V minute), namely per minute air flow is 0.12 times of reaction solution volume, carries out bioreduction, and the reaction times is 85 hours; After reaction terminates, first leach cell, then leach cotton gauze, with the extraction into ethyl acetate reaction solution 2 times of 0.3 times of reaction volume, with the extraction into ethyl acetate cotton gauze 3 times of 3 times of cotton gauze quality, the ethyl acetate of combined ethyl acetate extraction liquid and extraction gauze, steams ethyl acetate, obtains product ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters, reaction conversion ratio 96%, product yield 94%, enantiomeric excess rate (ee%) 98%.
embodiment 3
Nutrient solution nutrient solution composition is: glucose 19 g/L, starch 14g/L, analysis for soybean powder 28.5 g/L, yeast extract 6.5 g/L, tween 80 0.7g/L, KH
2pO
42.2 g/L, MgSO
4.7H
2o 0.35 g/L, soya-bean oil 25 mL/L, malt extract 28 g/L.Solid medium composition: add the agar powder of 2% in liquid medium within.
Wet yeast cell preparation process is as follows: antarctic candida through inclined-plane, shaking flask, seed tank culture obtain seed liquor; 500L fermentor tank adds nutrient solution, coefficient is 0.65,121 DEG C of autoclavings 30 minutes, are cooled to 24.5 DEG C, yeast starter liquid are seeded to fermentor tank, inoculative proportion is 9.5%, ventilation ratio is 1.2V/(V minute), namely per minute air flow is 1.2 times of reaction solution volumes, cultivates 52 hours for 24.5 DEG C, wet yeast cell is obtained, as bioreduction catalyzer with filtering centrifuge is centrifugal.
The cotton gauze making method of immunoabsorbent substrate is, substrate is dissolved in ethyl acetate, the ratio of substrate and ethyl acetate is 0.5:2, obtain the ethyl acetate solution of substrate, absorb this solution with the cotton gauze being of a size of 0.5cmX0.5cm, then, dry, reclaim ethyl acetate, namely obtain the cotton gauze of immunoabsorbent substrate, regulate substrate and cotton gauze consumption, make the quality ratio of substrate and cotton gauze be 0.31.
Phosphate buffered saline buffer is added in 1000L bottom ventilation stirred tank, coefficient is 0.65, pH is 6.3, add the cotton gauze of immunoabsorbent substrate, making substrate 2-(4-chlorobenzoyl)-2-methyl acrylate addition is 85 g/L, glucose content is 8.5g/L, wood sugar 6.5g/L, glyceryl monostearate content is 13g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 24.5 DEG C, add wet yeast cell and make concentration be 23g/L, ventilation ratio is 0.11V/(V minute), namely per minute air flow is 0.112 times of reaction solution volume, carries out bioreduction, and the reaction times is 82 hours; After reaction terminates, first leach cell, then leach cotton gauze, with the extraction into ethyl acetate reaction solution 2 times of 0.3 times of reaction volume, with the extraction into ethyl acetate cotton gauze 3 times of 3 times of cotton gauze quality, the ethyl acetate of combined ethyl acetate extraction liquid and extraction gauze, steams ethyl acetate, obtains product ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters, reaction conversion ratio 95%, product yield 93%, enantiomeric excess rate (ee%) 97.5%.
embodiment 4
Nutrient solution nutrient solution composition is: glucose 19 g/L, starch 14g/L, analysis for soybean powder 28.5 g/L, yeast extract 7 g/L, tween 80 0.8 g/L, KH
2pO
42.4 g/L, MgSO
4.7H
2o 0.4 g/L, soya-bean oil 25 mL/L, malt extract 30 g/L.Solid medium composition: add the agar powder of 2% in liquid medium within.
Wet yeast cell preparation process is as follows: antarctic candida through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, coefficient is 0.7,121 DEG C of autoclavings 30 minutes, are cooled to 25 DEG C, yeast starter liquid are seeded to fermentor tank, inoculative proportion is 10%, ventilation ratio is 1.3V/(V minute), namely per minute air flow is 1.3 times of reaction solution volumes, cultivates 54 hours for 25 DEG C, wet yeast cell is obtained, as bioreduction catalyzer with filtering centrifuge is centrifugal.
The cotton gauze making method of immunoabsorbent substrate is, substrate is dissolved in ethyl acetate, the ratio of substrate and ethyl acetate is 0.5:2, obtain the ethyl acetate solution of substrate, absorb this solution with the cotton gauze being of a size of 0.5cmX0.5cm, then, dry, reclaim ethyl acetate, namely obtain the cotton gauze of immunoabsorbent substrate, regulate substrate and cotton gauze consumption, make the quality ratio of substrate and cotton gauze be 0.3-0.32.
Phosphate buffered saline buffer is added in 5000L bottom ventilation stirred tank, coefficient is 0.7, pH is 6.3, add the cotton gauze of immunoabsorbent substrate, making substrate 2-(4-chlorobenzoyl)-2-methyl acrylate addition is 90 g/L, glucose content is 9g/L, wood sugar 7 g/L, glyceryl monostearate content is 15g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 25 DEG C, add wet yeast cell and make concentration be 25g/L, ventilation ratio is 0.12V/(V minute), namely per minute air flow is 0.12 times of reaction solution volume, carries out bioreduction, and the reaction times is 85 hours; After reaction terminates, first leach cell, then leach cotton gauze, with the extraction into ethyl acetate reaction solution 2 times of 0.3 times of reaction volume, with the extraction into ethyl acetate cotton gauze 3 times of 3 times of cotton gauze quality, the ethyl acetate of combined ethyl acetate extraction liquid and extraction gauze, steams ethyl acetate, obtains product ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters, reaction conversion ratio 96%, product yield 94%, enantiomeric excess rate (ee%) 97.5%.
Claims (4)
1. antarctic candida cell biological reduction preparation ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-) method of-2 Methylpropionic acid methyl esters, it is characterized in that adding phosphate buffered saline buffer in retort, coefficient is 0.6-0.7, pH is 6.3, add the cotton gauze of immunoabsorbent substrate, making substrate 2-(4-chlorobenzoyl)-2-methyl acrylate addition is 80-90 g/L, glucose content is 8-9g/L, wood sugar 6-7 g/L, glyceryl monostearate content is 12-15g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 24-25 DEG C, add wet yeast cell and make concentration be 22-25g/L, ventilation ratio is 0.10-0.12V/(V minute), carry out bioreduction, the reaction times is 80-85 hour; After reaction terminates, leach cell, cotton gauze respectively, be extracted with ethyl acetate reaction solution, extraction gauze, the ethyl acetate of combined ethyl acetate extraction liquid and extraction gauze, steams ethyl acetate, obtains product ()-(2R, 3S)-3-hydroxyl-3-(4-chloro-phenyl-)-2 Methylpropionic acid methyl esters, reaction conversion ratio 94-96%, product yield 92-94%, enantiomeric excess rate (ee%) 97-98%.
2. method according to claim 1, is characterized in that wet yeast cell preparation process is as follows: antarctic candida through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, coefficient is 0.6-0.7,121 DEG C of autoclavings 30 minutes, be cooled to 24-25 DEG C, yeast starter liquid is seeded to fermentor tank, inoculative proportion is 9-10%, ventilation ratio is 1-1.3V/(V minute), cultivate 49-54 hour, obtain wet yeast cell, as bioreduction catalyzer with filtering centrifuge is centrifugal for 24-25 DEG C.
3. method according to claim 1, is characterized in that nutrient solution composition is: glucose 18-20 g/L, starch 13-15g/L, analysis for soybean powder 28-29 g/L, yeast extract 6-7 g/L, tween 80 0.6-0.8 g/L, KH
2pO
42-2.4 g/L, MgSO
4.7H
2o 0.3-0.4 g/L, soya-bean oil 20-25 mL/L, malt extract 26-30 g/L; Solid medium composition: add the agar powder of 2% in liquid medium within.
4. method according to claim 1, it is characterized in that the cotton gauze making method of described immunoabsorbent substrate is, substrate is dissolved in ethyl acetate, and the ratio of substrate and ethyl acetate is 0.5:2, obtains the ethyl acetate solution of substrate, this solution is absorbed with the cotton gauze being of a size of 0.5cmX0.5cm, then, dry, reclaim ethyl acetate, namely obtain the cotton gauze of immunoabsorbent substrate, regulate substrate and cotton gauze consumption, make the quality ratio of substrate and cotton gauze be 0.3-0.32.
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| CN115043721A (en) * | 2022-06-01 | 2022-09-13 | 山东师范大学 | Preparation method of alpha-substituted chiral carboxylic acid compound |
| CN115043721B (en) * | 2022-06-01 | 2024-03-26 | 山东师范大学 | Preparation method of alpha-substituted chiral carboxylic acid compound |
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