CN104328119A - Microsatellite molecular marker related to growth character of megalobrama amblycephala and application of molecular marker - Google Patents
Microsatellite molecular marker related to growth character of megalobrama amblycephala and application of molecular marker Download PDFInfo
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- CN104328119A CN104328119A CN201410654344.4A CN201410654344A CN104328119A CN 104328119 A CN104328119 A CN 104328119A CN 201410654344 A CN201410654344 A CN 201410654344A CN 104328119 A CN104328119 A CN 104328119A
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Abstract
The invention discloses a microsatellite molecular marker related to growth character of megalobrama amblycephala and application of the molecular marker. Through clustering separation method, association analysis on the molecular marker and character is realized, two molecular markers obviously related to growth characters are finally screened; a primer of Mam116 is as follows: F: 5'-CTATTTACAGTTTCATGCTTTCCTC-3' and R: 5'-ATCCCGTCCGCCGCTTACT-3'; a primer of Mam166 is as follows: F: 5'-GGTACTGTTTGTGCTGGGC-3' and R: 5'-CTGCTCACTCAACTTATTGTAGGTC-3'. According to the molecular marker, multiple comparisons on different genotype characters are carried out on the two markers, so as to determine inferior and superior alleles, and individuals containing positive genotypes are selected from megalobrama amblycephala character related breeding as breeding parents while individuals containing negative genotypes are avoided, so as to reduce the experimental workload and accelerate the breeding efficiency.
Description
Technical field
The present invention relates to fish molecular marking supplementary breeding technology, be specifically related to the microsatellite molecular marker relevant to Megalobrama amblycephala growth traits and application.
Background technology
Megalobrama amblycephala (Megalobrama amblycephala Yih, 1955), has another name called blunt snout bream, is under the jurisdiction of Osteichthyes, Cypriniformes, Cyprinidae, and triangular bream belongs to, and its NATURAL DISTRIBUTION is only confined in a few lake, the middle and lower reach of Yangtze River.Because its delicious flavour, feeding habits are wide, growth soon, easily fish for and extensively like by human consumer and culturist.Because the inbred phenomenon of ubiquity in the production of Megalobrama amblycephala fingerling causes kind of the growth speed of the conventional seed of matter degeneration aggravation slower.Although China has selected No. 1, the Megalobrama amblycephala Pujiang growing that speed, seed still can not have been met the need of market.Need to strengthen the monitoring of Megalobrama amblycephala germ plasm resource, assessment and protection further, select and grow Megalobrama amblycephala improved seeds (Wang Weimin, 2009) that are fast, good stress resistance.
The growth traits of fish is quantitative character, and be multiple Gene Handling, these genes occupy certain region on chromosome, is called quantitative trait locus (Quantitative Trait Locus).The QTL of a quantitative character is not a lot, there is major gene (Li Ning, 1997).One or two major gene of these QTL just can reflect more than the 10%-50% of a quantitative trait phenotypes variation.Therefore we by finding the gene or mark that control these proterties, can carry out genetic improvement to the economic characters of fish.Microsatellite DNA, also known as simple tandem repetitive sequence (simple sequence repeat, SSR), be the nucleotide sequence of elementary cell repeatedly tandem sequence repeats by 2 ~ 6 bases, be distributed widely in (Hamad a et al. in Eukaryotic genome, 1982), because it has codominant inheritance, easy to detect, polymorphism information content advantages of higher, be widely used in aspect (Sun Xiaowen, 2004 such as the analysis of aquatic animal population genetic variations, the structure of genetic linkage maps, QTL location and marker assisted selection breeding; Liao et al., 2009).Zhang Yifeng etc. (2008) utilize from genetic linkage maps and delivered 47 pairs of micro-satellite primers to Bai Shi carp and the cold-resistant strain of C carpiovarwuyuanensis from F2 generation carry out body weight, body grows and height has carried out correlation analysis, result finds 7 sites relevant to growth traits, and finds the genotype that 3 kinds of proterties are relevant.Liu Xiande etc. (2013) screen 2 to the microsatellite marker that large yellow croaker growth traits is closely related and corresponding preponderant genotype thereof.
Conventional seed selection selects according to the phenotype of proterties instead of genotype, and lack preliminary election means to objective trait, breeding cycle is long, efficiency is low.The development of modern molecular biology technique makes to carry out being selected to possibility according to the gene relevant to proterties or molecule marker.Have not yet to see the report of Megalobrama amblycephala growth traits related molecular marker.
Summary of the invention
The object of the present invention is to provide microsatellite molecular marker Mam116 and Mam166 that Megalobrama amblycephala growth traits is relevant, the primer of Mam116 is F:CTATTTACAGTTTCATGCTTTCCTC, R:ATCCCGTCCGCCGCTTACT; The primer of Mam166 is F:GGTACTGTTTGTGCTGGGC, R:CTGCTCACTCAACTTATTGTAGGTC; This molecule marker is that the QTL of Megalobrama amblycephala growth traits locates and molecular mark work lays the foundation.
Another object of the present invention there are provided the application of the microsatellite molecular marker relevant to Megalobrama amblycephala growth traits in Megalobrama amblycephala marker assisted selection, namely based on the individuality that the advantage allelotrope screening growth traits of molecule marker is excellent.
The present invention is achieved by the following technical solutions:
The microsatellite molecular marker relevant to Megalobrama amblycephala growth traits, is obtained by following steps screening:
In June, (1) 2009, maternal to 42 tails from Liangzi Lake, Yuni Lake, Poyang Lake, 50 tail male parents carry out artificial propagation, and breeding filial generation cultivates in same pond.The Megalobrama amblycephala F1 generation that in March, 2011 salvages in pond is at random numbered, and measures body length, height and body weight.According to weight data, the great individuality in 0.4kg of selective body is designated as the individuality that large group of individuals (Top) and body weight be less than 0.28kg and is designated as little group of individuals (Below), each 96 tails;
(2) isozyme of the Megalobrama amblycephala F1 generation experimental subjects of clip step (1), adopts ammonium acetate method to extract the genomic dna of isozyme as template, selects 17 pairs of micro-satellite primers to carry out pcr amplification to microsatellite locus;
(3) the individual PCR primer at 17 microsatellite locus of Megalobrama amblycephala F1 generation is through the polymorphism of 8% (W/V) native polyacrylamide gel electrophoresis and cma staining method detection site, utilize number of alleles (the number of alleles of each microsatellite locus of PopGene (Version 3.2) software statistics, Na) and observation heterozygosity (Observed heterozygosity, Ho).Utilize the cognation of the GLM general linear model in SAS software to Megalobrama amblycephala growth traits and microsatellite locus to carry out Least square analysis, production traits indicator difference significance between same marker genetype is tested and carried out multiple comparisons;
(4) by general linear model, correlation analysis is carried out to the growth traits of Megalobrama amblycephala and 17 pairs of microsatellite markers, found that in 17 microsatellite locus, site Mam116, Mam166 all show significant correlation (P<0.05) with body length, body weight, height;
(5) multiple comparison analyse is carried out to the genotype in 2 sites, determine Pros and Cons allelotrope, finally determine that the preponderant genotype of Mam116 is 172/176,154/176,176/176 and 176/182; The preponderant genotype of Mam166 is 129/129,123/123,123/129 and 123/135.
The application of microsatellite molecular marker in Megalobrama amblycephala marker assisted selection that Megalobrama amblycephala growth traits is correlated with, application process is: analyze individual genotype, preponderant genotype by whether containing Mam116 site and Mam166 site judges whether individuality is the individuality that growth traits is excellent, thus parent is in selection.
Compared with prior art, the present invention has the following advantages:
(1) the present invention adopts partition method of hiving off, breed from criticizing, be standard with body weight in the Megalobrama amblycephala of raising with the pool, have selected body weight and be greater than each 96 tails of individuality that the individuality of 0.4kg and body weight be less than 0.28kg and analyze, thus realize the association analysis of molecule marker and proterties.
(2) the present invention increases the size of a sample of large group of individuals and little group of individuals, to obtaining the mark relevant to proterties more quickly and accurately.
(3) the present invention carries out the multiple comparisons of different genotype proterties to the mark that 2 and body length, body weight, height exist pole significant difference, reduces cut-and-try work amount to a certain extent, saves certain human and material resources, financial resources simultaneously.
(4) the present invention carries out the multiple comparisons of different genotype proterties to 2 marks, to determine inferior position and advantage allelotrope, final utilization marker assisted selection, can selecting containing just imitating genotypic individuality as seed selection parent in practice process as far as possible, avoiding containing the genotypic individuality of negative effect.
Embodiment
Technical scheme of the present invention, if not otherwise specified, is the ordinary skill in the art.
Embodiment 1:
The screening of microsatellite marker relevant to Megalobrama amblycephala growth traits:
(1) material source
In June, 2009, maternal to 42 tails from Liangzi Lake, Yuni Lake, Poyang Lake, 50 tail male parents carry out artificial propagation, and breeding filial generation cultivates in same pond.In March, 2011 salvages the Megalobrama amblycephala F1 generation in pond at random, numbering, and measure body length, height and weight data, clip fin ray is stored in 100% alcohol for subsequent use.According to weight data, selective body is great in the individuality of 0.4kg, is designated as large group of individuals (Top), and body weight is less than each 96 tails of individuality of 0.28kg, is designated as little group of individuals (Below), carries out micro-satellite gene type assay as experimental subjects; Simultaneously using individual for 60 tails of each group large microcommunity as microsatellite locus preliminary screening relevant to Megalobrama amblycephala growth traits, using the large microcommunity that each group of other 36 individualities are verified as the microsatellite locus that is associated with Megalobrama amblycephala growth traits.
(2) micro-satellite primers
In the present invention, 17 pairs of micro-satellite primers are for shown in table 1, and primer synthesizes by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.
17 pairs of micro-satellite primers information in table 1 the present invention
T: annealing temperature; Na: number of alleles; Ho: observation heterozygosity.
(3) extraction of Megalobrama amblycephala STb gene
Adopt ammonium acetate method to extract the DNA of Megalobrama amblycephala isozyme, the DNA of extraction detects its integrity and concentration by 1% agarose gel electrophoresis and Nan oDrop 2000 ultramicrospectrophotometer, and-20 DEG C save backup.
(4) amplification of microsatellite DNA
Each PCR reaction system cumulative volume is 20 μ l, comprising 10 × buffer (containing Mg
2+) 2.0 μ l, dNTPs (10m mol) 0.4 μ l, upstream and downstream primer (10 μm of ol) each 0.5 μ l, Taq enzyme 1U, DNA profiling is about 100ng.PCR reaction conditions is: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30s, primer renaturation temperature 30s, 72 DEG C extend 45s, 35 circulations; 72 DEG C extend 10min.Amplified production detects its polymorphism by 8% (W/V) native polyacrylamide gel electrophoresis and cma staining.
(5) data statistics and analysis
With number of alleles (number of alleles, Na) and observation heterozygosity (Observed heterozygosity, Ho) of each microsatellite locus of PopGene (Version 3.2) software statistics.Utilize the cognation of the GLM general linear model in SAS software to Megalobrama amblycephala growth traits and microsatellite locus to carry out Least square analysis, production traits indicator difference significance between same marker genetype is tested and carried out multiple comparisons.Observed value is less than the genotype of 4 times, because lacking break-up value, in this analysis that do not take statistics.
(6) preliminary screening of microsatellite locus relevant to Megalobrama amblycephala growth traits
1) polymorphism of microsatellite locus
This experiment used 17 can expand stable band clearly to micro-satellite.204 allelotrope detected in 17 sites altogether, wherein the polymorphism of site Mam144, Mam12, Mam46 is the highest, and number of alleles is all more than 14, and heterozygosity is all greater than 0.8.The polymorphism of site Mam147 is lower, and the number of alleles in two groups is respectively 5.The number of alleles of large group of individuals is between 5 ~ 17, and average number of alleles is 9.5, and heterozygosity is between 0.5658 ~ 0.8928, and average heterozygosity is 0.7627.The number of alleles of little group of individuals is between 4 ~ 18, and average number of alleles is 10.2, and heterozygosity is between 0.4489 ~ 0.8851, and average heterozygosity is 0.7150.The genetic diversity of two groups does not have significant difference.
2) correlation analysis of Megalobrama amblycephala microsatellite marker and growth traits
General linear model is utilized to carry out correlation analysis to the growth traits of Megalobrama amblycephala and 17 pairs of microsatellite markers, found that in 17 microsatellite locus, site Mam166 and Mam116 all shows pole significant correlation (P<0.01) with body length, body weight, height.
Embodiment 2:
The genotypic positives and negatives of site Mam166 and Mam116:
Multiple comparisons is carried out to the genotype in 2 sites, infers the inferior position allelotrope on each microsatellite markers, thus tentatively judge that above genotype plays proterties or the effect of plus or minus.
The genotype in site of the present invention is as the criterion with shown stripe size (bp) after Quantity One software reading site PCR primer polyacrylamide gel electrophoresis and cma staining.Such as site Mam116,121/121 genotype refers to the band only being amplified 121bp by primer; 154/176 genotype refers to go out 154bp and 176 liang band by primer amplification.
Multiple comparison analyse discovery is carried out to the genotype in 2 sites, in the Mam116 of site, obtain 11 kinds of genotype altogether, wherein 121/121,121/129, in body weight, significant difference (P>0.05) is not had between 121/133 genotype, but significantly lower than other genotype (P<0.05); 176/182 except with 154/176,176/176,172/176 without (P>0.05) outside the significance difference opposite sex, be significantly higher than other genotype (P<0.05); 123/123,154/176,154/172,154/182,176/176,172/176,172/180 between any two without significant difference (P>0.05).In body length with height, 154/172,154/182,172/180,176/176,172/176,154/176,123/123, significant difference (P>0.05) is not had between 176/182, its phenotypic number is significantly higher than 121/121,121/129,121/133 genotype individuals (P<0.05); Be significantly higher than 121/129 (P<0.05) in body long side 121/133, and there is no significant difference (P>0.05) between 121/121,121/129,121/133 genotype in height.
In the Mam166 of site, obtain 8 kinds of genotype altogether, in body weight, body length and height, 123/123,123/129,123/135, there is no significant difference (P>0.05) between 129/129 genotype, but be significantly higher than 261/269,261/279,269/269,269/279 genotype (P<0.05); In body weight and height, there was no significant difference (P>0.05) between 261/269,261/279,269/269,269/279 genotype; In body long side, 269/279 genotype is significantly lower than other genotype (P<0.05).The multiple comparisons of two individual phenotypes of site different genotype is as follows:
The multiple comparisons of the individual phenotype of table 2 site Mam116 different genotype
The multiple comparisons of the individual phenotype of table 3 site Mam166 different genotype
Note: in table 2 and table 3, in same row, same letter represents difference significantly (P>0.05), different letter representation significant difference (P<0.05).
Determine that the preponderant genotype of Mam116 is 172/176,154/176,176/176 and 176/182; The preponderant genotype of Mam166 is 129/129,123/123,123/129 and 123/135.
Embodiment 3:
The application of the microsatellite molecular marker relevant to Megalobrama amblycephala growth traits in Megalobrama amblycephala marker assisted selection, application process is as follows:
1) from the analytical results in two sites, choose 3 maximum positive-effect genotype of average respectively, minimum 3 the negative effect genotype of average, as choice criteria (deficiency is not got), the results are shown in Table 4.
The positive-effect in table 4 two sites and negative effect genotype
2) in 72 F1 generations (embodiment 1) individuality of checking colony, carry out gene type assay, the individuality chosen wherein containing plus or minus effector type carries out multiple comparisons and the significance of difference analysis of growth traits, the results are shown in Table 5.
The ontoanalysis of the plus or minus effector type of colony verified by table 5
Note: in same growth traits, same letter represents difference not significantly (P>0.05), different letter representation significant difference (P<0.05).N is the F1 generation number of individuals detected.
3) can see intuitively from result, in Mam116 and Mam166 two mark, as long as the growth traits value comprising preponderant genotype individuality will be significantly higher than the growth traits value with inferior position genotype individuals.Therefore Mam116 or Mam16 Marker selection can be utilized to contain and just to imitate genotypic individuality as seed selection parent, and avoid containing the genotypic individuality of negative effect, thus reduce the workload of breeding, improve breeding efficiency.
SEQUENCE LISTING
<110> Hua Zhong Agriculture University Hubei hundred holds aquatic products breeding company limited
The microsatellite molecular marker that <120> Megalobrama amblycephala growth traits is relevant and application
The microsatellite molecular marker that <130> Megalobrama amblycephala growth traits is relevant and application
<160> 4
<170> PatentIn version 3.1
<210> 1
<211> 25
<212> DNA
<213> artificial sequence
<400> 1
ctatttacag tttcatgctt tcctc 25
<210> 2
<211> 19
<212> DNA
<213> artificial sequence
<400> 2
atcccgtccg ccgcttact 19
<210> 3
<211> 19
<212> DNA
<213> artificial sequence
<400> 3
ggtactgttt gtgctgggc 19
<210> 4
<211> 25
<212> DNA
<213> artificial sequence
<400> 4
ctgctcactc aacttattgt aggtc 25
Claims (6)
1. the Megalobrama amblycephala growth traits primer of microsatellite molecular marker of being correlated with, Mam116:
F: 5’- CTATTTACAGTTTCATGCTTTCCTC -3’
R: 5’- ATCCCGTCCGCCGCTTACT -3’。
2. the Megalobrama amblycephala growth traits primer of microsatellite molecular marker of being correlated with, Mam166:
F: 5’- GGTACTGTTTGTGCTGGGC -3’
R: 5’- CTGCTCACTCAACTTATTGTAGGTC -3’。
3. the application of primer in Megalobrama amblycephala growth traits is correlated with breeding of the molecule marker described in claim 1 or 2.
4. application according to claim 3, described growth traits is the long proterties of body.
5. application according to claim 3, described growth traits is weight character.
6. application according to claim 3, described growth traits is height proterties.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554996A (en) * | 2016-08-31 | 2017-04-05 | 华中农业大学 | A kind of megalobrama amblycephala transferrin receptor genes SNP marker and its application |
| CN107190053A (en) * | 2017-03-13 | 2017-09-22 | 北京林业大学 | The combination of cypress microsatellite molecular marker, primer screening method and its application |
| CN107653323A (en) * | 2016-07-23 | 2018-02-02 | 华中农业大学 | Megalobrama amblycephala transferrins gene SNP molecular labeling and its application |
| CN110791511A (en) * | 2019-11-21 | 2020-02-14 | 上海海洋大学 | Hypoxia-resistant megalobrama amblycephala growth character gene and positioning method and application thereof |
| CN113528677A (en) * | 2021-08-11 | 2021-10-22 | 华中农业大学 | Leaf-specific notopterygium plateau loach microsatellite molecular marker, and primer and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487860A (en) * | 2011-11-24 | 2012-06-13 | 华中农业大学 | Method for screening megalobrama amblycephala group combinations with hybrid vigor |
| CN102653785A (en) * | 2011-03-03 | 2012-09-05 | 华中农业大学 | Identifying method of megalobrama amblycephala family by microsatellite |
-
2014
- 2014-11-17 CN CN201410654344.4A patent/CN104328119B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102653785A (en) * | 2011-03-03 | 2012-09-05 | 华中农业大学 | Identifying method of megalobrama amblycephala family by microsatellite |
| CN102487860A (en) * | 2011-11-24 | 2012-06-13 | 华中农业大学 | Method for screening megalobrama amblycephala group combinations with hybrid vigor |
Non-Patent Citations (2)
| Title |
|---|
| 罗伟: "团头鲂EST_SSR的开发及在育种中的应用", 《中国博士学位论文全文数据库-农业科技辑》 * |
| 罗伟等: "团头鲂微卫星多重PCR体系及应用", 《大连海洋大学学报》 * |
Cited By (8)
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| CN107653323A (en) * | 2016-07-23 | 2018-02-02 | 华中农业大学 | Megalobrama amblycephala transferrins gene SNP molecular labeling and its application |
| CN106554996A (en) * | 2016-08-31 | 2017-04-05 | 华中农业大学 | A kind of megalobrama amblycephala transferrin receptor genes SNP marker and its application |
| CN106554996B (en) * | 2016-08-31 | 2020-01-24 | 华中农业大学 | Megalobrama amblycephala transferrin receptor gene SNP molecular marker and application thereof |
| CN107190053A (en) * | 2017-03-13 | 2017-09-22 | 北京林业大学 | The combination of cypress microsatellite molecular marker, primer screening method and its application |
| CN110791511A (en) * | 2019-11-21 | 2020-02-14 | 上海海洋大学 | Hypoxia-resistant megalobrama amblycephala growth character gene and positioning method and application thereof |
| CN110791511B (en) * | 2019-11-21 | 2023-02-28 | 上海海洋大学 | Hypoxia-resistant megalobrama amblycephala growth character gene and positioning method and application thereof |
| CN113528677A (en) * | 2021-08-11 | 2021-10-22 | 华中农业大学 | Leaf-specific notopterygium plateau loach microsatellite molecular marker, and primer and application thereof |
| CN113528677B (en) * | 2021-08-11 | 2022-02-18 | 华中农业大学 | Leaf-specific notopterygium plateau loach microsatellite molecular marker, and primer and application thereof |
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