CN104312879A - Production process of fresh cordyceps militaris wine - Google Patents
Production process of fresh cordyceps militaris wine Download PDFInfo
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- CN104312879A CN104312879A CN201410644608.8A CN201410644608A CN104312879A CN 104312879 A CN104312879 A CN 104312879A CN 201410644608 A CN201410644608 A CN 201410644608A CN 104312879 A CN104312879 A CN 104312879A
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Abstract
The invention discloses a production process of fresh cordyceps militaris wine. The production process comprises the following concrete steps: culturing cordyceps militaris in a glass bottle, filling the bottle with just brewed fresh yellow wine when spores are not emitted during the fresh period of the cordyceps militaris, performing symbiotic ageing on a fresh cordyceps militaris culture medium, a fruit body and raw yellow wine for 85 days to synthesize a novel composite drink-the fresh cordyceps militaris wine. The main process is that the cordyceps militaris is cultured in the bottle; three main materials of hulless oat, wheat and unhusked rice in a formula are mixed, and especially edible and medicinal bitter herb is added, so that the side effect of dryness-heat of the raw yellow wine is eliminated; traditional yellow wine drunk by middle-aged and elderly people in winter is changed to novel healthcare yellow wine capable of being drunk by people of different classes all the year round; the method is easy and convenient, and the fresh cordyceps militaris wine can be conveniently produced and consumed by most people.
Description
Technical field
The present invention relates to food processing field, particularly the production method of novel nourishing wine that is combined into of a kind of fresh living cordyceps and traditional fermentation yellow rice wine, specifically a kind of production technique of fresh and alive cordyceps wine.
Background technology
No. 3 bulletin has been sent out from the Ministry of Health in 2009, since Cordyceps militaris (L.) Link. is listed in new resource food, (Cordyceps militaris (L.) Link. is Cordyceps militaris again, Cordyceps militaris (L.) Link. etc.) field that is applied to wine emerges in an endless stream, dry sporophore impregnated in white wine, fresh sporophore is combined with white wine and prepares, and with raw material yellow rice wine brewage, extracts Cordyccps-militaris-(L.)-link. Sporophore active ingredient and to be dissolved in wine etc.All not the product that fresh and alive Cordyceps militaris (L.) Link. is combined with yellow rice wine, more do not utilize substratum.In fact, in substratum except Cordyccps-militaris-(L.)-link. Sporophore remainder, 0.01% is no less than containing adenosine, be equivalent to the content of natural cordyceps sinensis, (" medicine " allusion quotation specifies that natural cordyceps sinensis is no less than the standard of 0.01% containing adenosine), also have many be of value to human body trace element and VITAMIN isoreactivity composition all abandon it with refuse.
The yellow rice wine of traditional fermentation, has the effect of natural health nourishing, and China is existing drinks history in 3000, mostly is the elderly and drinks winter, due to complex manufacturing technology, nowadays become factorial praluction, and main drawback is that nutrition is dull, easily gets angry after drink.
How traditional yellow rice wine is promoted as the popular four seasons drink with composite nutrient.Here it is is combined with traditional yellow rice wine with fresh living cordyceps, thus forms the reason place of novel product.As long as association kind of a Chinese caterpillar fungus will make aweto yellow wine, huge numbers of families are made to plant Chinese caterpillar fungus, huge numbers of families' health.
Summary of the invention
The object of the invention is the production technique in order to provide a kind of fresh and alive cordyceps wine, this technique just grow and just ripe fresh and alive Cordyccps-militaris-(L.)-link. Sporophore together with substratum and the fresh yellow rice wine (yellow rice wine of not yet ageing) just brewageed with ageing 85 days of dwelling in a bottle, Novel beverage-fresh living cordyceps health preserving wine can be become.
The present invention is achieved through the following technical solutions:
A production technique for fresh and alive cordyceps wine, comprises the steps:
1) preparation of nutritive medium
Get the bitter dish of 100-150g mixing major ingredient, 20-50g egg liquid, 2-3g glucose, 0.02-0.04mgVB1,0.02-0.05g magnesium sulfate and 2-4g, be placed in the water decocting and concentrating of 4 times of weight, until collecting by filtration to filtrate weight be mixing major ingredient weight 1.3-1.4 doubly, filtrate is now nutritive medium; Wherein, mix major ingredient by mass ratio be the naked oats of 1:1:1, wheat and paddy forms;
2) bottling sterilizing
Get mixing major ingredient to load in bottle, and add nutritive medium, after sealing at 121 DEG C autoclaving 1h, then at 100 DEG C normal-pressure sterilization 8h; Wherein, the add-on of nutritive medium is: 1g mixing major ingredient adds 1-1.75ml nutritive medium;
3) inoculation and a bacterium
Bottle after sterilizing cool to 30 DEG C time, then connect Cordyceps militaris kind in bottle, sealing is placed on sends out a bacterium room, sends out bacterium room lucifuge and keeps temperature to be 18-22 DEG C, and every day is sooner or later each ventilates once, and each 0.5h, sends out bacterium and cultivate 10-15 days; Wherein, the inoculum size of Cordyceps militaris kind is: 1g mixing major ingredient adds 0.333-0.625ml Cordyceps militaris kind;
4) vernalization with educate grass
After sending out bacterium good, bottle is moved into culturing room, mycelium stimulation sees light, illumination 200-300lux, and often day by day light is no less than 18h, and maintenance room temp is 14-25 DEG C, and every day is sooner or later each ventilates once, each 0.5h; When sporophore in bottle grows to 1cm, enter and educate the grass stage, illumination 500-2000lux, often day by day light is no less than 12h, and maintenance room temp is 18-25 DEG C, humidity is 75-95%, and the outer day and night temperature 10 DEG C of holding chamber, educates careless 45-50 days;
5) with yellow rice wine ageing altogether
When the sporophore in bottle grows to 4-8cm, top major part is also long when having the spore of thorn-like flower but do not bloom, and adds yellow rice wine immediately, and sealing ageing 85 days is namely drinkable afterwards; Wherein, the add-on of yellow rice wine is: 1g mixing major ingredient adds 16-21ml yellow rice wine.
Bacterial classification in present invention process is by the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, and preserving number is CGMCC NO.5577.
Cordycepin content >=0.1% in described sporophore (Cordyccps-militaris-(L.)-link. Sporophore) and stable content.
Described yellow rice wine is made a living yellow rice wine, has namely just brewageed the fresh yellow rice wine of not ageing.
The present invention specifically cultivates Cordyceps militaris (L.) Link. in vial, just when the fresh and alive phase of Chinese caterpillar fungus growth does not distribute spore, the fresh yellow rice wine just brewageed is poured in bottle, allows fresh living cordyceps substratum and sporophore and raw yellow rice wine commensalism ageing 85 days, synthesize a kind of new composite drink-fresh and alive cordyceps wine.Main technique cultivates Chinese caterpillar fungus in bottle, by naked oats, wheat, paddy three kinds of major ingredient mixing in batching, add the bitter dish of Dietotherapy double-purpose Chinese medicinal materials especially, eliminate the dryness-heat side effect of former yellow rice wine, change traditional yellow rice wine and used as multi-level people drinkable novel health care yellow rice wine throughout the year winter by the elderly.Method is easy, is conducive to broad masses' productive consumption.
By the fresh and alive cordyceps wine that present invention process is produced, detect the like product that its activeconstituents produces higher than other method, shown in table specific as follows through the Shanxi academy of traditional Chinese medicine:
| ? | Cordycepin | Adenosine | Polysaccharide | Cordycepin adenosine content % in substratum |
| Product of the present invention | 51.8ml/L | 30.7 | 0.08 | 0 |
| White wine and hay infusion product | 20.1ml/L | 10.2 | 0.01 | 0.4 |
| Fresh grass and white wine infusion product | 40.99ml/L | 20.72 | 0.01 | 0.02 |
Present invention process product obtains unusual effect in clinical application, and by the time of 1 year, people dissimilar more than 100 drank, and common reaction is: spirit, and appetite increases, and has no interest throughout the year, eliminates the side effect that traditional yellow rice wine is got angry especially.
Embodiment
Embodiment 1
A production technique for fresh and alive cordyceps wine, comprises the steps:
1) preparation of nutritive medium
Get the bitter dish of 100g mixing major ingredient, 20g egg liquid, 2g glucose, 0.02mgVB1,0.05g magnesium sulfate and 2g, be placed in the water decocting and concentrating of 4 times of weight, until collecting by filtration to filtrate weight be 1.3 times of mixing major ingredient weight, filtrate is now nutritive medium; Wherein, mix major ingredient by mass ratio be the naked oats of 1:1:1, wheat and paddy forms;
2) bottling sterilizing
Get 24g mixing major ingredient to load in bottle, and add 42ml nutritive medium, that is: the add-on of nutritive medium is: 1g mixing major ingredient adds 1.75ml nutritive medium, after sealing at 121 DEG C autoclaving 1h, then at 100 DEG C normal-pressure sterilization 8h;
3) inoculation and a bacterium
Bottle after sterilizing cool to 30 DEG C time, Cordyceps militaris kind 15ml is met again in bottle, that is: the inoculum size of Cordyceps militaris kind is: 1g mixing major ingredient inoculation 0.625ml Cordyceps militaris kind, sealing is placed on and sends out bacterium room again, send out bacterium room lucifuge and keep temperature to be 20 DEG C, once, each 0.5h, sends out a bacterium and cultivates 15 days the every day of each ventilation sooner or later;
4) vernalization with educate grass
After sending out bacterium good, bottle is moved into culturing room, mycelium stimulation sees light, illumination 300lux, and often day by day light is no less than 18h, keeps room temp to be 14 DEG C, and every day is sooner or later each ventilates once, each 0.5h; When sporophore in bottle grows to 1cm, enter and educate the grass stage, illumination 2000lux, often day by day light is no less than 12h, and maintenance room temp is 20 DEG C, humidity is 80%, and the outer day and night temperature 10 DEG C of holding chamber, educates grass 50 days;
5) with yellow rice wine ageing altogether
When the sporophore in bottle grows to 4-8cm, top major part is also long when having the spore of thorn-like flower but do not bloom, and add 500ml yellow rice wine immediately, that is: the add-on of yellow rice wine is: 1g mixing major ingredient adds 21ml yellow rice wine, and sealing ageing 85 days is namely drinkable afterwards.
Embodiment 2
A production technique for fresh and alive cordyceps wine, comprises the steps:
1) preparation of nutritive medium
Get the bitter dish of 130g mixing major ingredient, 40g egg liquid, 3g glucose, 0.04mgVB1,0.02g magnesium sulfate and 2g, be placed in the water decocting and concentrating of 4 times of weight, until collecting by filtration to filtrate weight be 1.4 times of mixing major ingredient weight, filtrate is now nutritive medium; Wherein, mix major ingredient by mass ratio be the naked oats of 1:1:1, wheat and paddy forms;
2) bottling sterilizing
Get 30g mixing major ingredient to load in bottle, and add 30ml nutritive medium, that is: the add-on of nutritive medium is: 1g mixing major ingredient adds 1ml nutritive medium, after sealing at 121 DEG C autoclaving 1h, then at 100 DEG C normal-pressure sterilization 8h;
3) inoculation and a bacterium
Bottle after sterilizing cool to 30 DEG C time, Cordyceps militaris kind 10ml is met again in bottle, that is: the inoculum size of Cordyceps militaris kind is: 1g mixing major ingredient inoculation 0.333ml Cordyceps militaris kind, sealing is placed on and sends out bacterium room again, send out bacterium room lucifuge and keep temperature to be 18 DEG C, once, each 0.5h, sends out a bacterium and cultivates 12 days the every day of each ventilation sooner or later;
4) vernalization with educate grass
After sending out bacterium good, bottle is moved into culturing room, mycelium stimulation sees light, illumination 240lux, and often day by day light is no less than 18h, keeps room temp to be 18 DEG C, and every day is sooner or later each ventilates once, each 0.5h; When sporophore in bottle grows to 1cm, enter and educate the grass stage, illumination 1000lux, often day by day light is no less than 12h, and maintenance room temp is 25 DEG C, humidity is 95%, and the outer day and night temperature 10 DEG C of holding chamber, educates grass 47 days;
5) with yellow rice wine ageing altogether
When the sporophore in bottle grows to 4-8cm, top major part is also long when having the spore of thorn-like flower but do not bloom, and add 480ml yellow rice wine immediately, that is: the add-on of yellow rice wine is: 1g mixing major ingredient adds 16ml yellow rice wine, and sealing ageing 85 days is namely drinkable afterwards.
Embodiment 3
A production technique for fresh and alive cordyceps wine, comprises the steps:
1) preparation of nutritive medium
Get the bitter dish of 150g mixing major ingredient, 30g egg liquid, 3g glucose, 0.02mgVB1,0.04g magnesium sulfate and 4g, be placed in the water decocting and concentrating of 4 times of weight, until collecting by filtration to filtrate weight be 1.3 times of mixing major ingredient weight, filtrate is now nutritive medium; Wherein, mix major ingredient by mass ratio be the naked oats of 1:1:1, wheat and paddy forms;
2) bottling sterilizing
Get 28g mixing major ingredient to load in bottle, and add 34ml nutritive medium, that is: the add-on of nutritive medium is: 1g mixing major ingredient adds 1.21ml nutritive medium, after sealing at 121 DEG C autoclaving 1h, then at 100 DEG C normal-pressure sterilization 8h;
3) inoculation and a bacterium
Bottle after sterilizing cool to 30 DEG C time, Cordyceps militaris kind 14ml is met again in bottle, that is: the inoculum size of Cordyceps militaris kind is: 1g mixing major ingredient inoculation 0.5ml Cordyceps militaris kind, sealing is placed on and sends out bacterium room again, send out bacterium room lucifuge and keep temperature to be 22 DEG C, once, each 0.5h, sends out a bacterium and cultivates 10 days the every day of each ventilation sooner or later;
4) vernalization with educate grass
After sending out bacterium good, bottle is moved into culturing room, mycelium stimulation sees light, illumination 200lux, and often day by day light is no less than 18h, keeps room temp to be 25 DEG C, and every day is sooner or later each ventilates once, each 0.5h; When sporophore in bottle grows to 1cm, enter and educate the grass stage, illumination 1500lux, often day by day light is no less than 12h, and maintenance room temp is 23 DEG C, humidity is 75%, and the outer day and night temperature 10 DEG C of holding chamber, educates grass 45 days;
5) with yellow rice wine ageing altogether
When the sporophore in bottle grows to 4-8cm, top major part is also long when having the spore of thorn-like flower but do not bloom, and add 495ml yellow rice wine immediately, that is: the add-on of yellow rice wine is: 1g mixing major ingredient adds 18ml yellow rice wine, and sealing ageing 85 days is namely drinkable afterwards.
Embodiment 4
A production technique for fresh and alive cordyceps wine, comprises the steps:
1) preparation of nutritive medium
Get the bitter dish of 125g mixing major ingredient, 50g egg liquid, 2g glucose, 0.03mgVB1,0.03g magnesium sulfate and 3g, be placed in the water decocting and concentrating of 4 times of weight, until collecting by filtration to filtrate weight be 1.4 times of mixing major ingredient weight, filtrate is now nutritive medium; Wherein, mix major ingredient by mass ratio be the naked oats of 1:1:1, wheat and paddy forms;
2) bottling sterilizing
Get 26g mixing major ingredient to load in bottle, and add 38ml nutritive medium, that is: the add-on of nutritive medium is: 1g mixing major ingredient adds 1.46ml nutritive medium, after sealing at 121 DEG C autoclaving 1h, then at 100 DEG C normal-pressure sterilization 8h;
3) inoculation and a bacterium
Bottle after sterilizing cool to 30 DEG C time, Cordyceps militaris kind 12ml is met again in bottle, that is: the inoculum size of Cordyceps militaris kind is: 1g mixing major ingredient inoculation 0.46ml Cordyceps militaris kind, sealing is placed on and sends out bacterium room again, send out bacterium room lucifuge and keep temperature to be 21 DEG C, once, each 0.5h, sends out a bacterium and cultivates 14 days the every day of each ventilation sooner or later;
4) vernalization with educate grass
After sending out bacterium good, bottle is moved into culturing room, mycelium stimulation sees light, illumination 280lux, and often day by day light is no less than 18h, keeps room temp to be 21 DEG C, and every day is sooner or later each ventilates once, each 0.5h; When sporophore in bottle grows to 1cm, enter and educate the grass stage, illumination 500lux, often day by day light is no less than 12h, and maintenance room temp is 18 DEG C, humidity is 90%, and the outer day and night temperature 10 DEG C of holding chamber, educates grass 48 days;
5) with yellow rice wine ageing altogether
When the sporophore in bottle grows to 4-8cm, top major part is also long when having the spore of thorn-like flower but do not bloom, and add 485ml yellow rice wine immediately, that is: the add-on of yellow rice wine is: 1g mixing major ingredient adds 19ml yellow rice wine, and sealing ageing 85 days is namely drinkable afterwards.
Claims (3)
1. a production technique for fresh and alive cordyceps wine, is characterized in that, comprises the steps:
1) preparation of nutritive medium
Get the bitter dish of 100-150g mixing major ingredient, 20-50g egg liquid, 2-3g glucose, 0.02-0.04mgVB1,0.02-0.05g magnesium sulfate and 2-4g, be placed in the water decocting and concentrating of 4 times of weight, until collecting by filtration to filtrate weight be mixing major ingredient weight 1.3-1.4 doubly, filtrate is now nutritive medium; Wherein, mix major ingredient by mass ratio be the naked oats of 1:1:1, wheat and paddy forms;
2) bottling sterilizing
Get mixing major ingredient to load in bottle, and add nutritive medium, after sealing at 121 DEG C autoclaving 1h, then at 100 DEG C normal-pressure sterilization 8h; Wherein, the add-on of nutritive medium is: 1g mixing major ingredient adds 1-1.75ml nutritive medium;
3) inoculation and a bacterium
Bottle after sterilizing cool to 30 DEG C time, then connect Cordyceps militaris kind in bottle, sealing is placed on sends out a bacterium room, sends out bacterium room lucifuge and keeps temperature to be 18-22 DEG C, and every day is sooner or later each ventilates once, and each 0.5h, sends out bacterium and cultivate 10-15 days; Wherein, the inoculum size of Cordyceps militaris kind is: 1g mixing major ingredient adds 0.333-0.625ml Cordyceps militaris kind;
4) vernalization with educate grass
After sending out bacterium good, bottle is moved into culturing room, mycelium stimulation sees light, illumination 200-300lux, and often day by day light is no less than 18h, and maintenance room temp is 14-25 DEG C, and every day is sooner or later each ventilates once, each 0.5h; When sporophore in bottle grows to 1cm, enter and educate the grass stage, illumination 500-2000lux, often day by day light is no less than 12h, and maintenance room temp is 18-25 DEG C, humidity is 75-95%, and the outer day and night temperature 10 DEG C of holding chamber, educates careless 45-50 days;
5) with yellow rice wine ageing altogether
When the sporophore in bottle grows to 4-8cm, top major part is also long when having the spore of thorn-like flower but do not bloom, and adds yellow rice wine immediately, and sealing ageing 85 days is namely drinkable afterwards; Wherein, the add-on of yellow rice wine is: 1g mixing major ingredient adds 16-21ml yellow rice wine.
2. the production technique of fresh and alive cordyceps wine according to claim 1, is characterized in that: cordycepin content >=0.1% in described sporophore and stable content.
3. the production technique of fresh and alive cordyceps wine according to claim 1 and 2, is characterized in that: described yellow rice wine is made a living yellow rice wine, has namely just brewageed the fresh yellow rice wine of not ageing.
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| CN201410644608.8A CN104312879B (en) | 2014-11-14 | 2014-11-14 | The production technique of fresh and alive cordyceps wine |
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| CN201410644608.8A CN104312879B (en) | 2014-11-14 | 2014-11-14 | The production technique of fresh and alive cordyceps wine |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154296A (en) * | 2015-07-15 | 2015-12-16 | 十堰神农武当医药科技园有限公司 | Preparation method of cordyceps wine |
| CN108707527A (en) * | 2018-03-16 | 2018-10-26 | 蒋丽霞 | High conidia powder cordyceps wine enjoys assembly production method and its culture assembly |
| CN109486622A (en) * | 2018-11-28 | 2019-03-19 | 湖南金芙农业科技有限公司 | Fresh and alive cordyceps wine and its cultural method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105154296A (en) * | 2015-07-15 | 2015-12-16 | 十堰神农武当医药科技园有限公司 | Preparation method of cordyceps wine |
| CN108707527A (en) * | 2018-03-16 | 2018-10-26 | 蒋丽霞 | High conidia powder cordyceps wine enjoys assembly production method and its culture assembly |
| CN109486622A (en) * | 2018-11-28 | 2019-03-19 | 湖南金芙农业科技有限公司 | Fresh and alive cordyceps wine and its cultural method |
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| Publication number | Publication date |
|---|---|
| CN104312879B (en) | 2016-04-13 |
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