CN104306519B - Anti-oxidation composite traditional Chinese medicine extraction isolate and preparation method thereof - Google Patents
Anti-oxidation composite traditional Chinese medicine extraction isolate and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of traditional Chinese medicine, and discloses an anti-oxidation composite traditional Chinese medicine extraction isolate and a preparation method thereof. The anti-oxidation composite traditional Chinese medicine extraction isolate comprises the following extraction isolates by weight: 4-8 parts of a purslane water extract, 6-14 parts of a malva verticillata l ethanol extract, 2-5 parts of a heartleaf houttuynia herb ethanol extract, and 5-10 parts of an oriental arborvilae seed supercritical extract. The anti-oxidation composite traditional Chinese medicine extraction isolate preparation method comprises water extraction, alcohol extraction, supercritical extraction, column separation and other steps. The prepared composite traditional Chinese medicine extraction isolate has the significant superoxide anion free radical removing effect so as to improve the anti-oxidation performance of the traditional Chinese medicine.
Description
Technical field
The invention belongs to the field of Chinese medicines, it is related to a kind of compound Chinese medicine and extracts separator and preparation method thereof, more particularly to
A kind of antioxidative compound Chinese medicine extracts separator and preparation method thereof.
Background technology
Free radical (free radical) is the mesostate of various biochemical reactions in human life activity, has height
The chemism of degree, is the effective system of defense of body, if certain level can not be maintained, can affect the vital movement of body.But
Free radical produces excessive and can not remove in time, it will life macromolecule material in attack plane body and various organelle,
Cause body in the various damages of molecular level, cellular level and histoorgan level, accelerate the senescence process of body and induce
Various diseases.
Ultra-oxygen anion free radical is from xanthine oxidase, the electron reduction effect release by enzyme for the nadph oxidase
Oxygen produce or be cracked to form by respiratory chain.
Free radical has the activity of height and extremely strong oxidation reaction ability, can attack internal life by Oxidation
Life macromole, such as nucleic acid, protein, saccharide and lipid etc., make these materials that peroxidating degeneration, crosslinked and fracture to occur, thus
Cause the destruction of cellularity and function, lead to disorganization and the degeneration change of body.The strong oxidation of free radical, damages
Hinder the life macromolecule of body, caused the miopragia of human body cell immunity and humoral immunization, ultimately result in immunological diseases
Occur.
The patent of Patent No. cn100450541 discloses the Health-protection traditional Chinese medicinal preparation with beauty treatment anti-oxidation efficacy, described
Technical scheme act on larger in terms of beauty treatment, and the effect in terms of antioxidation is relatively low.It is thus desirable to exploitation has higher resisting
The Chinese medicine extraction separator of Oxidation.
Content of the invention
Technical problem to be solved: there are a lot of compositions to have antioxidative effect in conventional Chinese medicine, but its antioxidation
Effect is all weaker to be needed to be extracted the Chinese medicine ingredients with antioxidation and separated it is impossible to effectively remove free radical,
Carry out reasonable combination again, to improve its antioxidative activity it is therefore desirable to a kind of antioxidative compound Chinese medicine extract separator and
Its preparation method.
Technical scheme: for the problems referred to above, the invention discloses a kind of antioxidative compound Chinese medicine extracts separator, described
Antioxidative compound Chinese medicine extract separator and include the extraction separator of following weight parts: Portulaca water extract is 4～8 parts,
Semen Abutili ethanol extraction is 6～14 parts, Herba Houttuyniae ethanol extraction is 2～5 parts, Semen Platycladi supercritical extract is 5～10
Part.
Preferably, a kind of described antioxidative compound Chinese medicine extracts separator, and the extraction including following weight parts separates
Thing: Portulaca water extract is 4～8 parts, Semen Abutili ethanol extraction is 6～14 parts, Herba Houttuyniae ethanol extraction is 2～5 parts, cypress
Sub- core supercritical extract is 5～10 parts, Herba Commelinae water extract is 5～12 parts, fevervine extracts separator and is 5～7 parts.
Preferably, a kind of described antioxidative compound Chinese medicine extracts separator, and the extraction including following weight parts separates
Thing: Portulaca water extract is 6 parts, Semen Abutili ethanol extraction is 9 parts, Herba Houttuyniae ethanol extraction is 4 parts, Semen Platycladi supercritical
Extract is 7 parts, Herba Commelinae water extract is 8 parts, fevervine extracts separator and is 5 parts.
A kind of antioxidative compound Chinese medicine extracts the preparation method of separator, and described compound Chinese medicine extracts the system of separator
Preparation Method is as follows: Herba Portulacae crushed after being dried, pulverizing are extracted by (1) with water, takes supernatant after extraction, by supernatant concentration
After obtain Portulaca water extract；(2) add ethanol to be extracted after pulverizing Semen Abutili, filter after extraction, filter vacuum is dense
It is Semen Abutili ethanol extraction after contracting；(3) Herba Houttuyniae is smashed, extracted with ethanol after smashing, centrifugation after extraction, will
The supernatant is concentrated to give Herba Houttuyniae ethanol extraction；(4) entered using supercritical carbon dioxide extraction method after Semen Platycladi being pulverized
Row extraction, collects the extract in separating still, is Semen Platycladi supercritical extract；(5) Herba Commelinae is pulverized, after pulverizing, use water logging
Carry, filter, being concentrated to give Herba Commelinae water extract；(6) fevervine is first extracted with water, after extraction, extracting solution is crossed silica gel
Post, collects effluent, obtains fevervine and extract separator after concentration；(7) Portulaca water extract is taken to be 4～8 parts, Semen Abutili by weight
Sub- ethanol extraction is 6～14 parts, Herba Houttuyniae ethanol extraction is 2～5 parts, Semen Platycladi supercritical extract is 5～10 parts, duck
Sole of the foot grass water extract is 5～12 parts, fevervine extracts separator and is 5～7 parts, is antioxidative after said extracted separator is mixed
Compound Chinese medicine extracts separator.
Preferably, a kind of described antioxidative compound Chinese medicine extracts the preparation method of separator, and described Herba Portulacae carries
Take thing preparation method as follows: by Herba Portulacae crushed after being dried, pulverizing is extracted with water, extraction temperature is room temperature, extraction time
For 4h, the weight of water is 4 times of Herba Portulacae weight, takes supernatant after extraction, will be concentrated in vacuo for supernatant after obtain Portulaca water
Extract.
Preferably, a kind of described antioxidative compound Chinese medicine extracts the preparation method of separator, described Semen Abutili second
Alcohol extracting thing preparation method is as follows: Semen Abutili is pulverized with pulverizer, adds ethanol to be extracted, Extracting temperature after pulverizing
For 50 DEG C, extraction time is 40min, and solid-liquid ratio is 1:7, by extracting solution filter-cloth filtering after extraction, after filtration carries out filtrate
After being concentrated in vacuo to no ethanol outflow, collect concentrate, concentrate is Semen Abutili ethanol extraction.
Preferably, a kind of described antioxidative compound Chinese medicine extracts the preparation method of separator, described Herba Houttuyniae second
Alcohol extracting thing preparation method is as follows: the Herba Houttuyniae after drying is smashed, is extracted with ethanol after smashing, Extracting temperature is 65
DEG C, extraction time is 30min, and solid-liquid ratio is 1:3, and supernatant is collected in centrifugation after extraction, obtains concentrated in vacuo for the supernatant
To Herba Houttuyniae ethanol extraction.
Preferably, a kind of described antioxidative compound Chinese medicine extracts the preparation method of separator, and described Semen Platycladi surpasses
The preparation method of critical extract is as follows: take Semen Platycladi, the moisture that Semen Platycladi is dried to Semen Platycladi is 3wt%, then by cypress
Sub- core is pulverized, and carries out supercritical carbon dioxide extraction after pulverizing, and extracting pressure is 30mpa, and flash trapping stage pressure is 8.5mpa, and two
Level separating pressure is 6.5mpa, and extraction time is 2h, and extraction temperature is 45 DEG C, collects the extraction in firsts and seconds separating still
Thing, is Semen Platycladi supercritical extract.
Preferably, a kind of described antioxidative compound Chinese medicine extracts the preparation method of separator, described Herba Commelinae water
The preparation method of extract is as follows: Herba Commelinae is pulverized, is stirred extracting with water, extraction temperature is room temperature, during extraction after pulverizing
Between be 120min, extraction solid-liquid ratio is 1:4, extracts and filters, is concentrated to give Herba Commelinae water extract after terminating.
Preferably, a kind of described antioxidative compound Chinese medicine extracts the preparation method of separator, and described fevervine carries
Take separator preparation method as follows: fevervine to be pulverized, be soaked in water after pulverizing 3h, extracted with water again after immersion, extract
Temperature is room temperature, and extraction time is 150min, and extraction solid-liquid ratio is 1:6, then extracting solution is filtered, and is filtrated to get supernatant；Will
The silicagel column of 300 mesh by activating for the supernatant, the blade diameter length ratio of silicagel column is 1:10, and loading flow velocity is 1bv/h, collects flow velocity
Also it is 1bv/h, effluent collects 100 pipes altogether, concentrates after last 30 pipe effluent are merged, and obtains fevervine and extracts separator.
In conventional Chinese medicine ingredients, some polysaccharide, flavone all have antioxidative effect, but its antioxidation is all relatively
Difference is it is impossible to effectively remove free radical.In the present invention, water extraction is carried out to Herba Portulacae and Herba Commelinae, obtained Herba Portulacae and the duck sole of the foot
Some active water soluble compositions in grass, such as polysaccharide etc..In addition also alcohol extraction is carried out to Semen Abutili and Herba Houttuyniae, obtain Semen Abutili
With some active component in Herba Houttuyniae.In addition also Semen Platycladi has been carried out with the activity that supercritical extraction obtains in Semen Platycladi non-
Polar component, is also extracted to the active component in fevervine and has been separated, and the compound Chinese medicine finally combined extracts
Separator has preferable antioxidation.
Beneficial effect: the ultra-oxygen anion free radical determining the antioxidative compound Chinese medicine extraction separator of the present invention is clear
Except rate.The compound Chinese medicine of the present invention extracts the free radical scavenging activity of separator 81.5% to 99.3%, all has significant antioxygen
The effect changed.Optimal compound Chinese medicine extracts the free radical scavenging activity highest of separator, has reached 99.3%.It is significantly higher than it
The clearance rate of his embodiment.
Specific embodiment
Embodiment 1
(1) by Herba Portulacae crushed after being dried, pulverizing is extracted with water, and extraction temperature is room temperature, and extraction time is 4h, water
Weight be 4 times of Herba Portulacae weight, take supernatant after extraction, will be concentrated in vacuo for supernatant after obtain Portulaca water extract；(2)
Semen Abutili is pulverized with pulverizer, adds ethanol to be extracted after pulverizing, Extracting temperature is 50 DEG C, and extraction time is
40min, solid-liquid ratio is 1:7, by extracting solution filter-cloth filtering after extraction, carries out being concentrated in vacuo to no ethanol stream by filtrate after filtration
After going out, collect concentrate, concentrate is Semen Abutili ethanol extraction；(3) Herba Houttuyniae after drying is smashed, after smashing, use ethanol
Extracted, Extracting temperature is 65 DEG C, extraction time is 30min, solid-liquid ratio is 1:3, supernatant is collected in centrifugation after extraction
Liquid, obtains Herba Houttuyniae ethanol extraction by concentrated in vacuo for the supernatant；(4) take Semen Platycladi, Semen Platycladi is dried to Semen Platycladi
Moisture is 3wt%, then Semen Platycladi is pulverized, and carries out supercritical carbon dioxide extraction after pulverizing, and extracting pressure is 30mpa, one
Level separating pressure is 8.5mpa, and the second-order separation pressure is 6.5mpa, and extraction time is 2h, and extraction temperature is 45 DEG C, collects one-level
With the extract in the second-order separation kettle, it is Semen Platycladi supercritical extract；(5) Herba Commelinae is pulverized, stirred with water after pulverizing
Mix extraction, extraction temperature is room temperature, extraction time is 120min, extraction solid-liquid ratio is 1:4, extraction is filtered after terminating, is concentrated to give
To Herba Commelinae water extract；(6) fevervine is pulverized, be soaked in water after pulverizing 3h, extracted with water again after immersion, Extracting temperature
For room temperature, extraction time is 150min, and extraction solid-liquid ratio is 1:6, then extracting solution is filtered, and is filtrated to get supernatant；By supernatant
The silicagel column of 300 mesh by activating for the liquid, the blade diameter length ratio of silicagel column is 1:10, and loading flow velocity is 1bv/h, collects flow velocity and is also
1bv/h, effluent collects 100 pipes altogether, concentrates after last 30 pipe effluent are merged, and obtains fevervine and extracts separator；(7) press
It is 6 parts that weight takes Portulaca water extract, Semen Abutili ethanol extraction is 9 parts, Herba Houttuyniae ethanol extraction is 4 parts, Semen Platycladi surpasses
Critical extract is 7 parts, Herba Commelinae water extract is 8 parts, fevervine extracts separator and is 5 parts, and said extracted separator is mixed
Extract separator for antioxidative compound Chinese medicine afterwards.
Embodiment 2
(1) by Herba Portulacae crushed after being dried, pulverizing is extracted with water, and extraction temperature is room temperature, and extraction time is 4h, water
Weight be 4 times of Herba Portulacae weight, take supernatant after extraction, will be concentrated in vacuo for supernatant after obtain Portulaca water extract；(2)
Semen Abutili is pulverized with pulverizer, adds ethanol to be extracted after pulverizing, Extracting temperature is 50 DEG C, and extraction time is
40min, solid-liquid ratio is 1:7, by extracting solution filter-cloth filtering after extraction, carries out being concentrated in vacuo to no ethanol stream by filtrate after filtration
After going out, collect concentrate, concentrate is Semen Abutili ethanol extraction；(3) Herba Houttuyniae after drying is smashed, after smashing, use ethanol
Extracted, Extracting temperature is 65 DEG C, extraction time is 30min, solid-liquid ratio is 1:3, supernatant is collected in centrifugation after extraction
Liquid, obtains Herba Houttuyniae ethanol extraction by concentrated in vacuo for the supernatant；(4) take Semen Platycladi, Semen Platycladi is dried to Semen Platycladi
Moisture is 3wt%, then Semen Platycladi is pulverized, and carries out supercritical carbon dioxide extraction after pulverizing, and extracting pressure is 30mpa, one
Level separating pressure is 8.5mpa, and the second-order separation pressure is 6.5mpa, and extraction time is 2h, and extraction temperature is 45 DEG C, collects one-level
With the extract in the second-order separation kettle, it is Semen Platycladi supercritical extract；(5) Herba Commelinae is pulverized, stirred with water after pulverizing
Mix extraction, extraction temperature is room temperature, extraction time is 120min, extraction solid-liquid ratio is 1:4, extraction is filtered after terminating, is concentrated to give
To Herba Commelinae water extract；(6) fevervine is pulverized, be soaked in water after pulverizing 3h, extracted with water again after immersion, Extracting temperature
For room temperature, extraction time is 150min, and extraction solid-liquid ratio is 1:6, then extracting solution is filtered, and is filtrated to get supernatant；By supernatant
The silicagel column of 300 mesh by activating for the liquid, the blade diameter length ratio of silicagel column is 1:10, and loading flow velocity is 1bv/h, collects flow velocity and is also
1bv/h, effluent collects 100 pipes altogether, concentrates after last 30 pipe effluent are merged, and obtains fevervine and extracts separator；(7) press
It is 8 parts that weight takes Portulaca water extract, Semen Abutili ethanol extraction is 6 parts, Herba Houttuyniae ethanol extraction is 2 parts, Semen Platycladi surpasses
Critical extract is 10 parts, Herba Commelinae water extract is 5 parts, fevervine extracts separator and is 6 parts, and said extracted separator is mixed
Extract separator for antioxidative compound Chinese medicine afterwards.
Embodiment 3
(1) by Herba Portulacae crushed after being dried, pulverizing is extracted with water, and extraction temperature is room temperature, and extraction time is 4h, water
Weight be 4 times of Herba Portulacae weight, take supernatant after extraction, will be concentrated in vacuo for supernatant after obtain Portulaca water extract；(2)
Semen Abutili is pulverized with pulverizer, adds ethanol to be extracted after pulverizing, Extracting temperature is 50 DEG C, and extraction time is
40min, solid-liquid ratio is 1:7, by extracting solution filter-cloth filtering after extraction, carries out being concentrated in vacuo to no ethanol stream by filtrate after filtration
After going out, collect concentrate, concentrate is Semen Abutili ethanol extraction；(3) Herba Houttuyniae after drying is smashed, after smashing, use ethanol
Extracted, Extracting temperature is 65 DEG C, extraction time is 30min, solid-liquid ratio is 1:3, supernatant is collected in centrifugation after extraction
Liquid, obtains Herba Houttuyniae ethanol extraction by concentrated in vacuo for the supernatant；(4) take Semen Platycladi, Semen Platycladi is dried to Semen Platycladi
Moisture is 3wt%, then Semen Platycladi is pulverized, and carries out supercritical carbon dioxide extraction after pulverizing, and extracting pressure is 30mpa, one
Level separating pressure is 8.5mpa, and the second-order separation pressure is 6.5mpa, and extraction time is 2h, and extraction temperature is 45 DEG C, collects one-level
With the extract in the second-order separation kettle, it is Semen Platycladi supercritical extract；(5) Herba Commelinae is pulverized, stirred with water after pulverizing
Mix extraction, extraction temperature is room temperature, extraction time is 120min, extraction solid-liquid ratio is 1:4, extraction is filtered after terminating, is concentrated to give
To Herba Commelinae water extract；(6) fevervine is pulverized, be soaked in water after pulverizing 3h, extracted with water again after immersion, Extracting temperature
For room temperature, extraction time is 150min, and extraction solid-liquid ratio is 1:6, then extracting solution is filtered, and is filtrated to get supernatant；By supernatant
The silicagel column of 300 mesh by activating for the liquid, the blade diameter length ratio of silicagel column is 1:10, and loading flow velocity is 1bv/h, collects flow velocity and is also
1bv/h, effluent collects 100 pipes altogether, concentrates after last 30 pipe effluent are merged, and obtains fevervine and extracts separator；(7) press
It is 4 parts that weight takes Portulaca water extract, Semen Abutili ethanol extraction is 14 parts, Herba Houttuyniae ethanol extraction is 5 parts, Semen Platycladi surpasses
Critical extract is 5 parts, Herba Commelinae water extract is 12 parts, fevervine extracts separator and is 7 parts, and said extracted separator is mixed
Extract separator for antioxidative compound Chinese medicine afterwards.
Embodiment 4
(1) by Herba Portulacae crushed after being dried, pulverizing is extracted with water, and extraction temperature is room temperature, and extraction time is 4h, water
Weight be 4 times of Herba Portulacae weight, take supernatant after extraction, will be concentrated in vacuo for supernatant after obtain Portulaca water extract；(2)
Semen Abutili is pulverized with pulverizer, adds ethanol to be extracted after pulverizing, Extracting temperature is 50 DEG C, and extraction time is
40min, solid-liquid ratio is 1:7, by extracting solution filter-cloth filtering after extraction, carries out being concentrated in vacuo to no ethanol stream by filtrate after filtration
After going out, collect concentrate, concentrate is Semen Abutili ethanol extraction；(3) Herba Houttuyniae after drying is smashed, after smashing, use ethanol
Extracted, Extracting temperature is 65 DEG C, extraction time is 30min, solid-liquid ratio is 1:3, supernatant is collected in centrifugation after extraction
Liquid, obtains Herba Houttuyniae ethanol extraction by concentrated in vacuo for the supernatant；(4) take Semen Platycladi, Semen Platycladi is dried to Semen Platycladi
Moisture is 3wt%, then Semen Platycladi is pulverized, and carries out supercritical carbon dioxide extraction after pulverizing, and extracting pressure is 30mpa, one
Level separating pressure is 8.5mpa, and the second-order separation pressure is 6.5mpa, and extraction time is 2h, and extraction temperature is 45 DEG C, collects one-level
With the extract in the second-order separation kettle, it is Semen Platycladi supercritical extract；(5) Portulaca water extract is taken to be 8 parts, Semen Abutili by weight
Sub- ethanol extraction is 6 parts, Herba Houttuyniae ethanol extraction is 2 parts, Semen Platycladi supercritical extract is 10 parts, and said extracted is divided
Extract separator for antioxidative compound Chinese medicine after thing mixing.
Embodiment 5
(1) by Herba Portulacae crushed after being dried, pulverizing is extracted with water, and extraction temperature is room temperature, and extraction time is 4h, water
Weight be 4 times of Herba Portulacae weight, take supernatant after extraction, will be concentrated in vacuo for supernatant after obtain Portulaca water extract；(2)
Semen Abutili is pulverized with pulverizer, adds ethanol to be extracted after pulverizing, Extracting temperature is 50 DEG C, and extraction time is
40min, solid-liquid ratio is 1:7, by extracting solution filter-cloth filtering after extraction, carries out being concentrated in vacuo to no ethanol stream by filtrate after filtration
After going out, collect concentrate, concentrate is Semen Abutili ethanol extraction；(3) Herba Houttuyniae after drying is smashed, after smashing, use ethanol
Extracted, Extracting temperature is 65 DEG C, extraction time is 30min, solid-liquid ratio is 1:3, supernatant is collected in centrifugation after extraction
Liquid, obtains Herba Houttuyniae ethanol extraction by concentrated in vacuo for the supernatant；(4) take Semen Platycladi, Semen Platycladi is dried to Semen Platycladi
Moisture is 3wt%, then Semen Platycladi is pulverized, and carries out supercritical carbon dioxide extraction after pulverizing, and extracting pressure is 30mpa, one
Level separating pressure is 8.5mpa, and the second-order separation pressure is 6.5mpa, and extraction time is 2h, and extraction temperature is 45 DEG C, collects one-level
With the extract in the second-order separation kettle, it is Semen Platycladi supercritical extract；(5) Portulaca water extract is taken to be 4 parts, Semen Abutili by weight
Sub- ethanol extraction is 14 parts, Herba Houttuyniae ethanol extraction is 5 parts, Semen Platycladi supercritical extract is 5 parts, and said extracted is divided
Extract separator for antioxidative compound Chinese medicine after thing mixing.
Ultra-oxygen anion free radical clearance rate measures
Take centrifuge tube, be separately added into the tris hydrochloride buffer 5ml of the ph=7.5 of 0.05mol/l, be separately added into embodiment 1
Compound Chinese medicine to 5 extracts the aqueous solution 0.5ml of separator, and mass fraction is 5wt%.Water-bath at 25 DEG C after mix homogeneously
15min.The pyrogallol solution at once adding 0.4mmol/l after taking-up is 4.5ml.The dimension life of 10wt% is added after reaction 3min
Plain c solution 0.05ml carrys out terminating reaction.At 420nm, after 10min, measure the ultraviolet absorptivity of each embodiment, reference examples are with pure
Water 0.3ml is as testing sample.Calculate the difference of reference examples and the absorbance of embodiment 1 to 5, then by difference divided by reference examples
Absorbance be clearance rate.Carry out contrast experiment simultaneously, contrast experiment's Chinese medicine be three seven weight be 4 parts, Fructus Crataegi be 5 parts, red
Join for 6 parts, Fructus Schisandrae Chinensis be 3 parts, obtain Chinese medicine extraction liquid with decocting after being endured, clearance rate assay method is ibid.Measurement result is as follows.
The ultra-oxygen anion free radical clearance rate of embodiment 1 to 5 and comparative example is respectively as follows: 99.3%, 90.3%, 89.6%,
81.5%、82.4%、66.8%.
As can be seen that the compound Chinese medicine of embodiment 1 to 5 extracts separator all has very high free radical scavenging activity, all
There is significant antioxidative effect.The compound Chinese medicine of embodiment 1 extracts the free radical scavenging activity highest of separator, reaches
99.3%.It is significantly higher than the clearance rate of embodiment 2 to 5.The ultra-oxygen anion free radical clearance rate of comparative example is minimum, only
66.8%.
Claims (7)
1. a kind of antioxidative compound Chinese medicine extracts the preparation method of separator it is characterised in that described compound Chinese medicine extracts and divides
As follows from the preparation method of thing: (1), by Herba Portulacae crushed after being dried, is extracted with water after pulverizing, takes supernatant after extraction, will
Portulaca water extract is obtained after supernatant concentration；(2) add ethanol to be extracted after pulverizing Semen Abutili, filter after extraction, will
Filter vacuum is Semen Abutili ethanol extraction after concentrating；(3) Herba Houttuyniae is smashed, extracted with ethanol after smashing, after extraction
Centrifugation, the supernatant is concentrated to give Herba Houttuyniae ethanol extraction；(4) adopt supercritical titanium dioxide after Semen Platycladi being pulverized
Carbon extraction is extracted, and collects the extract in separating still, is Semen Platycladi supercritical extract；(5) Herba Commelinae is pulverized, powder
With flooding after broken, filter, be concentrated to give Herba Commelinae water extract；(6) fevervine is first extracted with water, will be extracted after extraction
Liquid crosses silicagel column, collects effluent, obtains fevervine and extract separator after concentration；(7) take by weight Portulaca water extract be 4～
8 parts, Semen Abutili ethanol extraction be 6～14 parts, Herba Houttuyniae ethanol extraction be 2～5 parts, Semen Platycladi supercritical extract be 5
～10 parts, Herba Commelinae water extract be 5～12 parts, fevervine extract separator be 5～7 parts, by said extracted separator mix after
Extract separator for antioxidative compound Chinese medicine.
2. a kind of antioxidative compound Chinese medicine according to claim 1 extract separator preparation method it is characterised in that
Described Herba portulacae extract preparation method is as follows: Herba Portulacae crushed after being dried is extracted with water after pulverizing, extraction temperature
For room temperature, extraction time is 4h, and the weight of water is 4 times of Herba Portulacae weight, takes supernatant after extraction, and supernatant is concentrated in vacuo
After obtain Portulaca water extract.
3. a kind of antioxidative compound Chinese medicine according to claim 1 extract separator preparation method it is characterised in that
Described Semen Abutili ethanol extraction preparation method is as follows: Semen Abutili is pulverized with pulverizer, adds ethanol to enter after pulverizing
Row extracts, and Extracting temperature is 50 DEG C, and extraction time is 40min, and solid-liquid ratio is 1:7, by extracting solution filter-cloth filtering, mistake after extraction
After filter, filtrate is carried out being concentrated in vacuo to after no ethanol outflow, collect concentrate, concentrate is Semen Abutili ethanol extraction.
4. a kind of antioxidative compound Chinese medicine according to claim 1 extract separator preparation method it is characterised in that
Described Herba Houttuyniae ethanol extraction preparation method is as follows: the Herba Houttuyniae after drying smashed, extracted with ethanol after smashing,
Extracting temperature is 65 DEG C, and extraction time is 30min, and solid-liquid ratio is 1:3, centrifugation after extraction, collects supernatant, and upper strata is clear
Liquid is concentrated in vacuo to obtain Herba Houttuyniae ethanol extraction.
5. a kind of antioxidative compound Chinese medicine according to claim 1 extract separator preparation method it is characterised in that
The preparation method of described Semen Platycladi supercritical extract is as follows: takes Semen Platycladi, the moisture that Semen Platycladi is dried to Semen Platycladi contains
Measure as 3wt%, then Semen Platycladi is pulverized, after pulverizing, carry out supercritical carbon dioxide extraction, extracting pressure is 30mpa, flash trapping stage
Pressure is 8.5mpa, and the second-order separation pressure is 6.5mpa, and extraction time is 2h, and extraction temperature is 45 DEG C, collects firsts and seconds
Extract in separating still, is Semen Platycladi supercritical extract.
6. a kind of antioxidative compound Chinese medicine according to claim 1 extract separator preparation method it is characterised in that
The preparation method of described Herba Commelinae water extract is as follows: Herba Commelinae is pulverized, is stirred extracting with water, extraction temperature after pulverizing
For room temperature, extraction time is 120min, and extraction solid-liquid ratio is 1:4, and extraction is filtered after terminating, is concentrated to give Herba Commelinae water extract.
7. a kind of antioxidative compound Chinese medicine according to claim 1 extract separator preparation method it is characterised in that
It is as follows that described fevervine extracts separator preparation method: fevervine is pulverized, be soaked in water after pulverizing 3h, uses water after immersion again
Extracted, Extracting temperature is room temperature, extraction time is 150min, and extraction solid-liquid ratio is 1:6, then extracting solution is filtered, and filters
Obtain supernatant；By the silicagel column of 300 mesh by activating for the supernatant, the blade diameter length ratio of silicagel column is 1:10, and loading flow velocity is
1bv/h, collecting flow velocity is also 1bv/h, and effluent collects 100 pipes altogether, concentrates after last 30 pipe effluent are merged, and obtains chicken arrow
Rattan extracts separator.
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| CN110075565A (en) * | 2019-04-18 | 2019-08-02 | 福建中西农业科技有限公司 | A kind of method of cordate houttuynia extraction preparation |
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