CN104297378B - A kind of sorbose sweat detects and fermentation termination determination methods - Google Patents
A kind of sorbose sweat detects and fermentation termination determination methods Download PDFInfo
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- CN104297378B CN104297378B CN201410598175.7A CN201410598175A CN104297378B CN 104297378 B CN104297378 B CN 104297378B CN 201410598175 A CN201410598175 A CN 201410598175A CN 104297378 B CN104297378 B CN 104297378B
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- sorbose
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- fermentation termination
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- 238000000855 fermentation Methods 0.000 title claims abstract description 41
- 230000004151 fermentation Effects 0.000 title claims abstract description 41
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 14
- 210000004243 sweat Anatomy 0.000 title claims abstract description 11
- 239000013558 reference substance Substances 0.000 claims abstract description 16
- 238000001514 detection method Methods 0.000 claims abstract description 11
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 11
- 239000012498 ultrapure water Substances 0.000 claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims abstract description 7
- 238000009826 distribution Methods 0.000 claims abstract description 7
- 239000000600 sorbitol Substances 0.000 claims abstract description 7
- 239000006228 supernatant Substances 0.000 claims abstract description 7
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000010812 external standard method Methods 0.000 claims abstract description 5
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 5
- 239000001257 hydrogen Substances 0.000 claims abstract description 5
- 238000001032 ion-exclusion chromatography Methods 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 22
- 238000001556 precipitation Methods 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000012488 sample solution Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 2
- 238000005265 energy consumption Methods 0.000 abstract description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 230000003373 anti-fouling effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/365—Ion-exclusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/047—Standards external
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8886—Analysis of industrial production processes
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A kind of sorbose sweat detects and fermentation termination determination methods, comprises the following steps: 1) reference substance solution preparation: by ultrapure water precision preparation reference substance solution; 2) sample preparation: get a step sorbose fermented broth 5ml in Vc production, adds 5ml containing ZnSO
4and K
4fe (CN)
6solution, centrifugal heavy shallow lake, gets supernatant, dilutes with ultrapure water; 3) chromatographic condition: chromatographic column: hydrogen shape ion exclusion chromatography post; Mobile phase: ultrapure water; Detecting device: Composition distribution; 4) measure: the content calculating the sorbose, sorbierite etc. of testing sample by external standard method; 5) fermentation termination judge: monitor fermentation process, when sorbose content no longer raise and sorbitol content lower than 0.5mg/ml, can fermentation termination be judged as.The present invention is easy to operate, improves accuracy and the work efficiency of detection, reduces fermentation energy consumption and production cost.
Description
technical field:
The present invention relates to Vc production field, particularly relate to a kind of sorbose sweat and detect and fermentation termination determination methods.
background technology:
During tradition Vc produces, one-step fermentation is raw material with sorbierite, and bacterial fermentation changes into sorbose.In sweat, the content detection of sorbose takes copper glycerolate titrimetry, and sorbierite only uses refractometry detection level before inoculation, without monitoring in sweat; Fermentation termination owing to cannot detect the amount of residual sorbierite in fermentation liquor, but does not increase as criterion by detecting sorbose content.The method metrical error detecting reducing sugar due to copper glycerolate is larger; general about 5%; by force empirical; different operating personnel Yi Fasheng deviation, for guaranteeing the accuracy detected, usually can adopt in fermentation liquor repeatedly to sample and rechecking; duplicate acknowledgment; length consuming time, causes fermentation termination to judge to delay, increases fermentation energy consumption.
summary of the invention:
The object of this invention is to provide a kind of sorbose sweat to detect and fermentation termination determination methods, more accurately and efficiently the process of one-step fermentation in Vc production run is detected, the content of sorbose and sorbierite in fermentation liquor can be detected, monitor fermentation process simultaneously, and judge fermentation termination.
The present invention is achieved in that a kind of sorbose sweat detects and fermentation termination determination methods, comprises the following steps: 1) reference substance solution preparation: by the reference substance solution of ultrapure water precision preparation containing sorbose 25mg/ml, sorbierite 1mg/ml; 2) sample preparation: get a step sorbose fermented broth 5ml in Vc production, adds 5ml containing ZnSO
420-60mg/ml and K
4fe (CN)
6the solution of 10-30mg/ml, mixing, pours centrifugal precipitation mechanism precipitation into, gets supernatant, with ultrapure water dilute above-mentioned supernatant to 2-20 doubly after, pour metre filter into; 3) chromatographic condition: chromatographic column: hydrogen shape ion exclusion chromatography post, column temperature: 60-85 DEG C; Mobile phase: be modulated into sulfuric acid the ultrapure water that pH equals 2, flow rate of mobile phase: 0.4-1.0ml/min; Detecting device: Composition distribution, Composition distribution sample cell temperature: in 25 DEG C-50 DEG C; 4) measure: by reference substance solution and sample solution sample detection, according to reference substance appearance time, peak area, calculate the content of the sorbose, sorbierite etc. of testing sample by external standard method; 5) fermentation termination judge: monitor fermentation process, when sorbose content no longer raise and sorbitol content lower than 0.5mg/ml, can fermentation termination be judged as.
Centrifugal precipitation mechanism rotating speed of the present invention is 12000rpm.
Filtering head of filter aperture of the present invention is 0.22 micron.
The present invention is easy to operate, improves accuracy and the work efficiency of detection, reduces fermentation energy consumption and production cost.
embodiment:
Following examples are only for illustration of the present invention, but the present invention is not by the restriction of these embodiments.
embodiment 1:
Preparation, containing sorbose 25mg/ml, sorbierite 1mg/ml reference substance solution, prepares mobile phase; Get the middle sample 5ml of one-step fermentation in Vc production, add 5ml containing ZnSO
4(40mg/ml) and K
4fe (CN)
6(20mg/ml) solution, mixing, rotating speed 12000rpm centrifugal settling, gets supernatant ultrapure water and dilutes 20 times, filters with 0.22 Mm filter head; Sample detection, sample size is 20 microlitres; Chromatographic condition: flow velocity is 0.8ml/min; Column temperature is 60 DEG C; Composition distribution sample cell temperature is 35 DEG C; Chromatographic column: match point scientific and technological CarbomixH10:8%7.8*300mm; Measure: by reference substance solution and sample solution sample detection, according to reference substance appearance time, peak area, calculate the content of the sorbose, sorbierite etc. of testing sample by external standard method, monitor fermentation process judges that fermentation termination sorbitol content value is 0.47mg/ml.
embodiment 2:
Preparation, containing sorbose 25mg/ml, sorbierite 1mg/ml reference substance solution, prepares mobile phase.Get one-step fermentation terminal sample 5ml in Vc production, add 5ml containing ZnSO
4(50mg/ml) and K
4fe (CN)
6(15mg/ml) solution, mixing, rotating speed 12000rpm centrifugal settling, gets supernatant ultrapure water and dilutes 5 times, and 0.22 Mm filter head filters.Sample detection, sample size 20 microlitre; Chromatographic condition: flow velocity 0.6ml/min; Column temperature 80 DEG C; Composition distribution sample cell temperature 35 DEG C; Chromatographic column: Shimadzu SCR-101H; Measure: by reference substance solution and sample solution sample detection, according to reference substance appearance time, peak area, calculate the content of the sorbose, sorbierite etc. of testing sample by external standard method, monitor fermentation process judges that fermentation termination sorbitol content value is as 0.45mg/ml.
By the fermentation termination sample of traditional endpoint method, detect by the inventive method, under regular situation, residual sorbitol content is lower than 1mg/ml, part batch residual sorbierite amount is up to more than 4mg/ml, sorbitol content is far away higher than 0.5mg/ml, the residual sorbierite of high concentration not only causes the waste of supplementary material, and have negative effect to the production of rear road, new detection method, can monitor fermentation process more accurately, judges fermentation termination more accurately, decrease the wasting of resources, improve product quality, convenient and swift, favorable reproducibility.The present invention is by adopting hydrogen shape chromatographic column, sodium or calcium shape ion exclusion chromatography post is adopted to compare with routine, degree of separation is suitable, appearance time is short, detect the used time few, the anti-fouling performance of hydrogen shape chromatographic column is far above sodium or calcium shape post (such as Shimadzu SCR-101C, match point CarbomixCa-NP) simultaneously, and price is also relatively low, more Multi-example number can be detected when detecting fermentation liquor, reducing testing cost.
The invention is not restricted to embodiment disclosed in these; the present invention is by the scope described by soverlay technique scheme; and the various distortion of scope required by right and equivalence change; under the prerequisite not departing from technical solution of the present invention, any amendment that those skilled in the art made for the present invention easily realize or improvement all belong to the present invention's scope required for protection.
Claims (3)
1. sorbose sweat detects and a fermentation termination determination methods, it is characterized in that: comprise the following steps: (1) reference substance solution is prepared: by the reference substance solution of ultrapure water precision preparation containing sorbose 25mg/ml, sorbierite 1mg/ml; (2) sample preparation: get a step sorbose fermented broth 5ml in Vc production, adds 5ml containing ZnSO
420-60mg/ml and K
4fe (CN)
6the solution of 10-30mg/ml, mixing, pours centrifugal precipitation mechanism precipitation into, gets supernatant, with ultrapure water dilute above-mentioned supernatant to 2-20 doubly after, pour metre filter into; (3) chromatographic condition: chromatographic column: hydrogen shape ion exclusion chromatography post, column temperature: 60-85 DEG C; Mobile phase: be modulated into sulfuric acid the ultrapure water that pH equals 2, flow rate of mobile phase: 0.4-1.0ml/min; Detecting device: Composition distribution, Composition distribution sample cell temperature: in 25 DEG C-50 DEG C; (4) measure: by reference substance solution and sample solution sample detection, according to reference substance appearance time, peak area, calculate the content of the sorbose, sorbierite etc. of testing sample by external standard method; (5) fermentation termination judge: monitor fermentation process, when sorbose content no longer raise and sorbitol content lower than 0.5mg/ml, can fermentation termination be judged as.
2. a kind of sorbose sweat according to claim 1 detects and fermentation termination determination methods, it is characterized in that: described centrifugal precipitation mechanism rotating speed is 12000rpm.
3. a kind of sorbose sweat according to claim 1 detects and fermentation termination determination methods, it is characterized in that: described filtering head of filter aperture is 0.22 micron.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410598175.7A CN104297378B (en) | 2014-10-31 | 2014-10-31 | A kind of sorbose sweat detects and fermentation termination determination methods |
| PCT/CN2015/093153 WO2016066117A1 (en) | 2014-10-31 | 2015-10-29 | A method of detection in the fermentation process of sorbose and judgment of the end of fermentation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410598175.7A CN104297378B (en) | 2014-10-31 | 2014-10-31 | A kind of sorbose sweat detects and fermentation termination determination methods |
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| CN104297378A CN104297378A (en) | 2015-01-21 |
| CN104297378B true CN104297378B (en) | 2015-12-30 |
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| WO (1) | WO2016066117A1 (en) |
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| CN104297378B (en) * | 2014-10-31 | 2015-12-30 | 帝斯曼江山制药（江苏）有限公司 | A kind of sorbose sweat detects and fermentation termination determination methods |
| CN106370603B (en) * | 2016-09-05 | 2018-10-12 | 安徽绿之鑫信息科技有限责任公司 | A method of judging bean curd of fermented fermentation termination using light sensor |
| CN109374767B (en) * | 2018-01-31 | 2021-06-08 | 合肥合源药业有限公司 | Method for detecting isosorbide mononitrate and isosorbide in composition of isosorbide mononitrate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101338335A (en) * | 2008-08-07 | 2009-01-07 | 江南大学 | Method for enhancing fermentation and production stability of 2-ketone-L-Gulonic acid |
| CN101475977A (en) * | 2008-12-31 | 2009-07-08 | 江南大学 | Method for controlling growth of Bacillus megatherium and promoting production of vitamin C |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0233050B1 (en) * | 1986-02-11 | 1992-09-16 | Takeda Chemical Industries, Ltd. | Method for producing l-sorbose |
| SG41916A1 (en) * | 1995-06-19 | 1997-08-15 | Univ Singapore | Determination of oxidant or reductant concentration by the spectrophotometric or visual response in oxidation or reduction of polyaniline |
| WO1999064560A1 (en) * | 1998-06-08 | 1999-12-16 | Basf Aktiengesellschaft | Methods for monitoring the stability of protein or cellular product secretion by cells in a fermenter culture |
| CA2726868A1 (en) * | 2008-06-13 | 2009-12-17 | Foss Analytical A/S | Process control of biotechnological processes |
| CN104297378B (en) * | 2014-10-31 | 2015-12-30 | 帝斯曼江山制药（江苏）有限公司 | A kind of sorbose sweat detects and fermentation termination determination methods |
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- 2014-10-31 CN CN201410598175.7A patent/CN104297378B/en active Active
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- 2015-10-29 WO PCT/CN2015/093153 patent/WO2016066117A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101338335A (en) * | 2008-08-07 | 2009-01-07 | 江南大学 | Method for enhancing fermentation and production stability of 2-ketone-L-Gulonic acid |
| CN101475977A (en) * | 2008-12-31 | 2009-07-08 | 江南大学 | Method for controlling growth of Bacillus megatherium and promoting production of vitamin C |
Non-Patent Citations (1)
| Title |
|---|
| 高效液相色谱法测定山梨糖发酵液中山梨糖和山梨醇;张宏霞等;《理化检验-化学分册》;20111231;第47卷(第10期);1239页1.1节-1240页2.4节 * |
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| Publication number | Publication date |
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| WO2016066117A1 (en) | 2016-05-06 |
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