CN104297213A - Blood cell analyzing instrument and recognizing method and system for abnormal cells thereof - Google Patents
Blood cell analyzing instrument and recognizing method and system for abnormal cells thereof Download PDFInfo
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- CN104297213A CN104297213A CN201310299584.2A CN201310299584A CN104297213A CN 104297213 A CN104297213 A CN 104297213A CN 201310299584 A CN201310299584 A CN 201310299584A CN 104297213 A CN104297213 A CN 104297213A
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Abstract
The invention discloses a recognizing method for abnormal cells. The method includes steps of: (1) per-grouping to-be-test cells; (2) obtaining a signal which reflects size information of the to-be-test cells and converting the signal into a first electronic pulse, and calculating to obtain an equivalent width of the first electronic pulse; and (3) comparing the equivalent width of the to-be-test cells in one cell group with a preset value of the cell group, if the equivalent width is more than the preset value, recognizing the to-be-test cells as the abnormal cells in the corresponding cell group. Because that an upper limit of the equivalent width of normal cells is certain, cells in the cell group, in which the equivalent width is more than the preset value, is recognized as the abnormal cells. By means of the method, accuracy of recognizing the abnormal cells can be improved. In addition, the blood cell recognizing method is carried out through calculation to obtain the equivalent width of the electronic pulse on the basis of a conventional light scattering method for obtaining the electronic pulse so that the method is free of individual detection channels, thereby reducing complex degree of the instrument and enabling the instrument to be more small-sized.
Description
Technical field
The present invention relates to blood analysis, particularly relate to a kind of blood cell analyzer and paracytic recognition methods thereof and system.
Background technology
Blood cell analyzer is generally used for and counts the red blood cell in blood, blood platelet, leucocyte and classify, wherein, five classification blood cell analyzers generally leucocyte can be divided into lymph, monokaryon, neutral grain, addicted to acid, basophilic five subgroups, when there is special-shaped abnormal lymphocytes and korocyte in leucocyte, blood cell analyzer has abnormal alarm prompting.
Usually, the method of leucocyte being carried out five classification is light scattering method, its ultimate principle uses the light scattering signal of two or more angles to detect the blood cell dynamically flow through in flow chamber, usually lower scattering angle signal reflects the size of cell, larger scattering angle signal reflects cell interior complexity, is classified to leucocyte by the scatter diagram of lower scattering angle signal and higher scattering angle signal acquisition.
When there being the abnormal cells such as special-shaped abnormal lymphocytes and korocyte in blood sample, scatter diagram there will be extra cell subsets.Due to abnormal cell and normal cell region overlap, adopt above-mentioned light scattering method to identify abnormal cell in blood and there is the low defect of accuracy.
Summary of the invention
Based on this, be necessary the paracytic recognition methods providing a kind of accuracy high.
A kind of paracytic recognition methods, comprises the following steps:
Cell to be measured is hived off in advance;
Obtain the signal of the described cell volume information to be measured of reflection and be converted into the first electric pulse, obtaining the first amplitude;
According to amplitude and the area of described first electric pulse, obtain the equivalent width of described first electric pulse;
The equivalent width of the cell to be measured in a cell mass and the preset value of described cell mass are compared;
If described equivalent width exceedes described preset value, be the abnormal cell in corresponding cell mass by described cell recognition to be measured.
Wherein in an embodiment, by the step that described cell to be measured hives off in advance be:
Obtain the signal of the described cell interior complexity information to be measured of reflection and be converted into the second electric pulse, obtaining the second amplitude;
According to described first amplitude and described second amplitude, described cell to be measured is hived off in advance.
Wherein in an embodiment, the signal of the described cell interior complexity information to be measured of described reflection comprises lateral scattering light signal, forward direction high angle scatter light signal, fluorescence signal, radiofrequency signal, or the signal of described reflection cell volume information comprises forward-scattering signal or electrical impedance signal.
Wherein in an embodiment, described cell to be measured is divided into lymphocyte, monocyte and granulocyte three groups by described hiving off in advance, or lymphocyte, monocyte, eosinophil and neutrophil leucocyte four groups, or lymphocyte, monocyte, basophilic granulocyte, eosinophil, neutrophil leucocyte five groups.
Wherein in an embodiment, if the equivalent width of the cell to be measured hived off in advance in lymphocyte populations exceedes lymphocytic preset value, be special-shaped abnormal lymphocytes by described cell recognition to be measured; Or
If the equivalent width of the cell to be measured hived off in advance in granulocyte group exceedes granulocytic preset value, be immature granulocyte by described cell recognition to be measured.
Wherein in an embodiment, according to area and the amplitude of described electric pulse, the equivalent width obtaining described electric pulse specifically comprises the following steps:
Obtain the signal of the described cell volume to be measured of reflection and convert described signal to electric pulse;
Gather the amplitude of described electric pulse;
To described electric pulse integration, obtain integrated value, described integrated value represents the area of described electric pulse;
By the amplitude of the area of described electric pulse divided by described electric pulse, obtain the equivalent width of described electric pulse.
Wherein in an embodiment, before described equivalent width and preset value comparison step, also comprise by theory calculate or add up Normocellular equivalent width, obtaining described preset value.
Wherein in an embodiment, reflect that the signal of described cell volume information to be measured is forward-scattering signal, the preferred forward direction of described forward-scattering signal low angle scattered light signal.
A kind of paracytic recognition system, comprising:
Pre-grouping module, hives off in advance by cell to be measured;
First amplitude module, obtains the signal of the described cell volume information to be measured of reflection and is converted into the first electric pulse, obtaining the first amplitude;
Equivalent width module, according to amplitude and the area of described first electric pulse, obtains the equivalent width of described first electric pulse;
Comparison module, compares the equivalent width of the cell to be measured in a cell mass and the preset value of described cell mass;
Described cell recognition to be measured, if described equivalent width exceedes described preset value, is the abnormal cell in corresponding cell mass by identification module.
Wherein in an embodiment, described pre-grouping module comprises:
Second amplitude unit, obtains the signal of the described cell interior complexity information to be measured of reflection and is converted into the second electric pulse, obtaining the second amplitude;
Pre-point group unit, hives off described cell to be measured in advance according to described first amplitude and described second amplitude.
Wherein in an embodiment, described cell to be measured is divided into lymphocyte, monocyte and granulocyte three groups by described pre-grouping module, or lymphocyte, monocyte, eosinophil and neutrophil leucocyte four groups, or lymphocyte, monocyte, basophilic granulocyte, eosinophil, neutrophil leucocyte five groups.
Wherein in an embodiment, if the equivalent width of the cell to be measured hived off in advance in lymphocyte populations exceedes lymphocytic preset value, described cell recognition to be measured is special-shaped abnormal lymphocytes by identification module; Or
If the equivalent width of the cell to be measured hived off in advance in granulocyte group exceedes granulocytic preset value, described cell recognition to be measured is immature granulocyte by identification module.
Wherein in an embodiment, described equivalent width module comprises:
Acquiring unit, obtains the signal of the described cell volume to be measured of reflection and converts described signal to electric pulse;
Collecting unit, gathers the amplitude of described electric pulse;
Integral unit, to described electric pulse integration, obtains integrated value;
Computing unit, by described integrated value divided by amplitude, obtains the equivalent width of described electric pulse.
Wherein in an embodiment, reflect that the signal of described cell volume information to be measured is forward-scattering signal or electrical impedance signal, the preferred forward direction of described forward-scattering signal low angle scattered light signal.
A kind of blood cell analyzer, comprises above-mentioned paracytic recognition system.
Above-mentioned blood cell analyzer and paracytic recognition methods thereof and system, first cell to be measured is hived off in advance, then based on the electric pulse of the signal conversion of reflection cell volume information to be measured, calculate the equivalent width of described electric pulse, when the equivalent width in a cell mass exceeds the preset value of corresponding cell mass, be identified as abnormal cell.The upper limit due to Normocellular equivalent width is certain, after cell to be measured hives off, when its equivalent width exceeds the preset value of corresponding cell mass, just abnormal cell can be identified.Therefore, above-mentioned abnormal cell recognition methods is adopted can to improve the accuracy of abnormal cell identification in blood cell.
In addition, above-mentioned abnormal cell recognition methods obtains on the basis of electric pulse at traditional light scattering method, utilize the light signal of reflection cell volume to be measured, by calculating the equivalent width obtaining electric pulse, do not need independent sense channel, thus reduce the complexity of instrument, reduce production cost, use cost or maintenance and repair cost.Meanwhile, above-mentioned abnormal cell recognition methods does not need to increase detection reagent yet, reduces testing cost.
Accompanying drawing explanation
Fig. 1 is the paracytic recognition methods process flow diagram of an embodiment;
Fig. 2 is leucocyte to be measured x on a time shaft in an embodiment
1-x
2between electric pulse figure;
Fig. 3 is the electric pulse figure of leucocyte to be measured under different angles signal in an embodiment;
Fig. 4 is the two-dimentional scatter diagram that in blood sample, leukocytic first amplitude and the second amplitude generate;
Fig. 5 is the two-dimentional scatter diagram that leukocytic first amplitude to be measured and the second amplitude generate;
Fig. 6 is the two-dimentional scatter diagram that in leucocyte to be measured, monocyte generates with the second amplitude and the corresponding equivalent width of neutrophil leucocyte;
Fig. 7 is the paracytic recognition system schematic diagram of an embodiment.
Embodiment
For enabling above-mentioned purpose of the present invention, feature and advantage become apparent more, are described in detail the specific embodiment of the present invention below in conjunction with accompanying drawing.Set forth a lot of detail in the following description so that fully understand the present invention.But the present invention can be much different from alternate manner described here to implement, those skilled in the art can when without prejudice to doing similar improvement when intension of the present invention, therefore the present invention is by the restriction of following public concrete enforcement.
In blood testing field, accurately identify the cell type of blood cell and identify abnormal cell, being conducive to understanding the relevant physiological parameter of blood.But, detect that abnormal cell directly can not draw the conclusion of whether disease, whether the abnormal reference etc. depending on paracytic quantity, abnormal morphology, other related physiological parameters.Therefore, paracytic recognition methods described below, does not belong to the Diagnosis and Treat method of disease.As shown in Figure 1, the paracytic recognition methods of an embodiment, comprises the following steps:
Step S110, hives off in advance by cell to be measured.First cell to be measured is divided into different groups, then identifies abnormal cell in each group.This avoid between different cell mass causes the accuracy rate identified to reduce because equivalent width is close.
Cell to be measured is hived off in advance, refraction, scattering, absorption light signal, electrical impedance signal and their combination in any can be adopted to hive off in advance to cell to be measured, according to the type and the needs that detect cell, cell to be measured can be divided into different cell masses.Wherein, in one embodiment, when cell to be measured is leucocyte, leucocyte can be divided into lymphocyte, monocyte and granulocyte three groups, or lymphocyte, monocyte, eosinophil and neutrophil leucocyte four groups, or divide lymphoblast, monocyte, basophilic granulocyte, eosinophil, neutrophil leucocyte five groups.
Step S120, obtains the signal of reflection cell volume information to be measured and is converted into the first electric pulse, obtaining the first amplitude.Because the first electric pulse also may be used for hiving off in advance of cell to be measured, therefore step S120 can before step S110.When blood cell is one by one by flow chamber, when photoelectric commutator receives the light signal of cell to be measured and converts electric pulse to, along with the movement of cell, signal intensity can change electric pulse.As shown in Figure 2, the horizontal ordinate x in Fig. 2 represents the time, and ordinate f (x) represents electric impulse signal intensity.Reflect that the signal of described cell volume information to be measured is forward-scattering signal or electrical impedance signal.Wherein, the square value of forward scattering light and cell dia is closely related, therefore can reflect the size of cell volume.In present embodiment, forward-scattering signal is reflect that the signal of cell volume information to be measured is forward direction low angle scattered light signal, usually, forward direction low angle scattered light signal refers to laser beam irradiation direction and the light signal becoming 0-5 degree between the photomultiplier transit optical axis direction direction of collecting scattered light signal.Forward-scattering signal also can use in the process of hiving off in advance, therefore this signal be can utilize and extra signal transmitting and receiving cable do not increased, only needing the data to obtaining to process, reducing hardware cost and also saving detection time simultaneously.
Step S130, according to amplitude and the area of the first electric pulse, obtains the equivalent width of the first electric pulse.
The pulse of same amplitude, equivalent width is large, and illustrate that top is more flat, entirety is fatter.In one embodiment, the acquisition methods of equivalent width comprises: obtain the signal of reflection cell volume to be measured and convert described signal to electric pulse, as the value of the f (x) of the squiggle in Fig. 2; Gather the amplitude of electric pulse, i.e. f
max; To described electric pulse integration, obtain integrated value; By integrated value divided by amplitude f
max, i.e. the amplitude of electric pulse, the numerical value obtained is equivalent width: concrete formula is as follows with reference to figure 2:
Easily expect also having other mathematical methods to obtain equivalent width, such as, based on this formula, to be multiplied by or divided by fixing coefficient or carry out derivative action etc. and be out of shape the equivalent width calculated and also may be used for the present embodiment.
In one embodiment, cell to be measured hived off in advance comprise the following steps:
Obtain the signal of reflection cell volume information to be measured and be converted into the first electric pulse, such as: the scattered light signal receiving reflection cell volume to be measured, and converting light signal to electric pulse, gather the amplitude of electric pulse, be recorded as the first amplitude; In the present embodiment, the information of hiving off in advance is the signal of the reflection cell volume to be measured utilized, acquisition equivalent width that can be easier.
Obtain the signal of reflection cell interior complexity information to be measured and be converted into the second electric pulse, obtaining the second amplitude; In one embodiment, reflect that the signal of cell interior complexity to be measured comprises lateral scattering light signal, forward direction high angle scatter light signal, fluorescence signal and radiofrequency signal.Wherein, lateral scattering light signal refers to the scattered light signal in 90 degree directions orthogonal with laser beam, and the refractive index of side scattered light cell membrane is more responsive, can reflect intracellular fine structure information to be measured.Forward direction high angle scatter light signal refers to laser beam irradiation direction and the light signal becoming 3-30 degree between the photomultiplier transit optical axis direction direction of collecting scattered light signal.
According to the first amplitude and described second amplitude, cell to be measured is hived off in advance, such as, according to the two-dimentional scatter diagram that the first amplitude and the second amplitude generate, just cell to be measured can be divided into different cell masses.
Step S140, compares the equivalent width of cell to be measured in a cell mass and the preset value of cell mass.After cell is hived off in advance, the equivalent width numerical value that different cell masses is corresponding different, the preset value of the equivalent width of cell to be measured and corresponding cell mass is compared, just can learn in cell to be measured whether there is abnormal cell, in one embodiment, before described equivalent width and preset value comparison step, also comprise by theory calculate or add up Normocellular equivalent width, obtaining described preset value.Such as, normal cell is measured, and the method for normal cell according to step S110 is hived off in advance, then the distribution of the equivalent width of each normal cell populations is added up, just can obtain Normocellular equivalent width, i.e. the preset value of corresponding cell mass.
Cell recognition to be measured, if equivalent width exceedes preset value, is the abnormal cell in corresponding cell mass by step S150.Each cell mass in rear discovery normal cell is added up to the equivalent width of Normocellular cell mass there is the obvious equivalent width upper limit, so when occurring that equivalent width is greater than the cell of the preset value of corresponding cell mass in cell to be measured, just abnormal cell can be identified.In one embodiment, when cell to be measured is leucocyte, and described leucocyte is divided into three groups, its paracytic recognition methods is:
If the equivalent width of the cell to be measured hived off in advance in lymphocyte populations exceedes lymphocytic preset value, be special-shaped abnormal lymphocytes by described cell recognition to be measured;
If the equivalent width of the cell to be measured hived off in advance in granulocyte group exceedes granulocytic preset value, be immature granulocyte by described cell recognition to be measured.
When leucocyte is divided into four groups or five groups, the abnormal cell in corresponding granulocyte group is just identified as the immature granulocyte of this group.
In one embodiment, step S150 can also take following methods: the second amplitude obtaining the electric pulse of reflection cell complexity to be measured; Scatter diagram is generated according to the second amplitude and equivalent width; When equivalent width exceeds the preset value of corresponding cell mass, be identified as abnormal cell.When there is abnormal cell in cell mass, its equivalent width will exceed the preset value of corresponding cell mass, will be identified on scatter diagram very intuitively.
Be described in detail with example more specifically below.
Blood cell instrument measures the leucocyte in blood cell, comprises the following steps:
When blood cell is one by one by flow chamber, photoelectric commutator receives the scattered light signal of reflection leucocyte volume, obtain the first angle (such as 0 ~ 5 degree) electric pulse, gather the amplitude of the first angle electric pulse, electric pulse is carried out integration, obtain integrated value, then use integrated value divided by amplitude, obtain equivalent width.
As shown in Figure 2, be the x on a time shaft of leucocyte in the present embodiment
1-x
2between electric pulse, its wave function is f (x), and its amplitude is the maximal value place F of waveform
max.
As shown in Figure 3, the first row waveform is the electric pulse that the second angle (the second angle is greater than the first angle, such as 3 ~ 30 degree) light signal obtains, and amplitude reflects intracellular complexity, mainly the complexity of cell interior; The structural representation of the second behavior cell; The third line is the electric pulse of the first angular light signal acquisition, the volume of amplitude reflection cell.As shown in Figure 3, the size of the first angle signal reacting cells volume, different cells pulse amplitude under the first angle is similar, but pulse shape is obviously different.Adopt the first angle electric pulse to calculate acquisition equivalent width, pulse shape is different, and the equivalent width obtained also can be different.The equivalent width that calculates of electric pulse obtained using the light signal of the first angle, as test parameter, can improve the accuracy of result.
Photoelectric commutator also receives the light signal of this leucocyte complexity of reflection simultaneously, obtains the second angle electric pulse, gathers the amplitude of the second angle electric pulse; After removing blood shadow, adopt the amplitude of the amplitude of the first angle electric pulse and the second angle electric pulse to generate two-dimentional scatter diagram, leucocyte to be measured is hived off in advance.In the present embodiment, as shown in Figure 4, Figure 5, wherein Fig. 4 is more normal blood sample to leukocytic two-dimentional scatter diagram.And as shown in Figure 5, the present embodiment measure leucocyte in there is abnormal cell, but due to abnormal cell core normal region overlapping, affect paracytic identification.
Then measure the equivalent width of different cell mass in leucocyte to be measured respectively, when equivalent width numerical value is exceeded preset value, be abnormal cell.Wherein, what separate in lymphocyte is special-shaped different lymphocyte, and what separate in neutrophil leucocyte, eosinophil, basophilic granulocyte is immature granulocyte.
Wherein the acquisition of the preset value of equivalent width can adopt and not measure containing paracytic normal blood sample or leucocyte simulation particle, first leucocyte is hived off, its two-dimentional scatter diagram obtained as shown in Figure 4, then every group of leukocytic equivalent widths are measured, and the equivalence of each group of cells is added up, obtain the equivalent width preset value of each group's cell.
Conveniently display utilizes equivalent width identification abnormal cell intuitively, and to adopt the second amplitude and equivalent width to generate two-dimentional scatter diagram, the abnormal cell being greater than preset value will very clearly show in scatter diagram.Because the signal of larger angle can reflect intracellular complexity, after leucocyte is hived off, the eucaryotic cell structure of each group's cell is relatively simple, now, adopt the second amplitude and equivalent width to generate two-dimentional scatter diagram, can equivalent width be utilized to a greater degree identify, improve the intuitive of scatter diagram.
As shown in Figure 6, be two-dimentional scatter diagram that the equivalent width of monocyte in the present embodiment and granulocytic second amplitude and its mensuration generates.As seen from Figure 6, when occurring korocyte in monocyte and granulocyte, the equivalent width of korocyte can exceed monocyte and granulocytic equivalent width preset value, therefore can clearly identify.
Adopt the method for the present embodiment, detect the known blood sample containing immature granulocyte, the immature granulocyte ratio of acquisition is 19.6%.The ratio that traditional microscopy method obtains immature granulocyte in this sample has 38 juvenile cells in 19%(people's number 200 cells).The result that this method detects is consistent with classic method.
Emphasis of the present invention be the equivalent width utilizing the electric pulse of the signal of reflection cell volume information to calculate identify hive off in advance after abnormal cell in certain cell mass.The first amplitude that the information being used for hiving off in advance can utilize volume information to obtain, also can not utilize the first amplitude, as long as obtain certain cell to divide group congruences in advance and the signal obtaining reflection cell volume information.Certainly, the scheme in the present embodiment is easier.The signal being used for hiving off in advance can be refraction, scattering, absorption light signal, electrical impedance signal and its combination in any, as long as leucocyte at least can be divided into three groups.
Paracytic recognition methods in above-mentioned leucocyte, on existing five classification blood cell analyzers, just increase the characteristic parameter of an equivalent width, and equivalent width is by calculating, therefore, do not need independent sense channel, thus reduce the complexity of instrument, reduce production cost, use cost and maintenance and repair cost.After being hived off by leucocyte, when occurring abnormal cell in leucocyte, paracytic equivalent width can exceed the equivalent width threshold value of corresponding cell mass, thus is identified by abnormal cell, improves the accuracy of identification.
In addition, as shown in Figure 7, additionally provide a kind of paracytic recognition system, comprise pre-grouping module 710, first amplitude module 720, equivalent width module 730, comparison module 740 and identification module 750.
Pre-grouping module 710, hives off in advance by cell to be measured.Pre-grouping module 710 is first divided into different groups cell to be measured, then identifies abnormal cell in each group.This avoid between different cell mass causes the accuracy rate identified to reduce because equivalent width is close.
Cell to be measured is hived off in advance, refraction, scattering, absorption light signal, electrical impedance signal and their combination in any can be adopted to hive off in advance to cell to be measured, according to the type and the needs that detect cell, cell to be measured can be divided into different cell masses.Wherein, in one embodiment, when cell to be measured is leucocyte, leucocyte can be divided into lymphocyte, monocyte and granulocyte three groups, or lymphocyte, monocyte, eosinophil and neutrophil leucocyte four groups, or divide lymphoblast, monocyte, basophilic granulocyte, eosinophil, neutrophil leucocyte five groups.
First amplitude module 720 obtains the signal of the described cell volume information to be measured of reflection and is converted into the first electric pulse, obtains the first amplitude.When blood cell is one by one by flow chamber, when photoelectric commutator receives the light signal of cell to be measured and converts electric pulse to, along with the movement of cell, signal intensity can change electric pulse.As shown in Figure 2, the horizontal ordinate x in Fig. 2 represents the time, and ordinate f (x) represents electric impulse signal intensity.Reflect that the signal of described cell volume information to be measured is forward-scattering signal or electrical impedance signal.Wherein, the square value of forward scattering light and cell dia is closely related, therefore can reflect the size of cell volume.In present embodiment, forward-scattering signal is reflect that the signal of cell volume information to be measured is forward direction low angle scattered light signal, usually, forward direction low angle scattered light signal refers to laser beam irradiation direction and the light signal becoming 0-5 degree between the photomultiplier transit optical axis direction direction of collecting scattered light signal.Forward-scattering signal also can use in the process of hiving off in advance, therefore this signal be can utilize and extra signal transmitting and receiving cable do not increased, only needing the data to obtaining to process, reducing hardware cost and also saving detection time simultaneously.
Equivalent width module 730, according to amplitude and the area of described first electric pulse, obtains the equivalent width of described first electric pulse.The pulse of same amplitude, equivalent width is large, and illustrate that top is more flat, entirety is fatter.In one embodiment, equivalent width module comprises: acquiring unit, obtains the signal of the described cell volume to be measured of reflection and converts described signal to electric pulse, as the value of the f (x) of the squiggle in Fig. 2; Collecting unit, gathers the amplitude of described electric pulse, i.e. f
max; Integral unit, to described electric pulse integration, obtains integrated value; Computing unit, by described integrated value divided by amplitude f
max, i.e. the amplitude of electric pulse, obtains the equivalent width of described electric pulse.Concrete formula is as follows with reference to figure 2:
Easily expect also having other mathematical methods to obtain equivalent width, such as, based on this formula, to be multiplied by or divided by fixing coefficient or carry out derivative action etc. and be out of shape the equivalent width calculated and also may be used for the present embodiment.
In one embodiment, pre-grouping module comprises the second amplitude unit and divides group unit in advance.
Second amplitude unit, obtains the signal of the described cell interior complexity information to be measured of reflection and is converted into the second electric pulse, obtaining the second amplitude; In one embodiment, reflect that the signal of cell interior complexity to be measured comprises lateral scattering light signal, forward direction high angle scatter light signal, fluorescence signal and radiofrequency signal.Wherein, lateral scattering light signal refers to the scattered light signal in 90 degree directions orthogonal with laser beam, and the refractive index of side scattered light cell membrane is more responsive, can reflect intracellular fine structure information to be measured.Forward direction high angle scatter light signal refers to laser beam irradiation direction and the light signal becoming 3-30 degree between the photomultiplier transit optical axis direction direction of collecting scattered light signal.
Pre-point group unit, hives off described cell to be measured in advance according to described first amplitude and described second amplitude, such as, according to the two-dimentional scatter diagram that the first amplitude and the second amplitude generate, just cell to be measured can be divided into different cell masses.
The equivalent width of the cell to be measured in a cell mass and the preset value of described cell mass compare by comparison module 740.After cell is hived off in advance, the equivalent width numerical value that different cell masses is corresponding different, the preset value of the equivalent width of cell to be measured and corresponding cell mass is compared, just can learn in cell to be measured whether there is abnormal cell, by theory calculate or Normocellular equivalent width can be added up, obtain described preset value.Such as, normal cell is measured, and hive off in advance, then the distribution of the equivalent width of each normal cell populations is added up, just can obtain Normocellular equivalent width, be i.e. the preset value of corresponding cell mass.
If described equivalent width exceedes described preset value, described cell recognition to be measured is the abnormal cell in corresponding cell mass by identification module 750.Each cell mass in rear discovery normal cell is added up to the equivalent width of Normocellular cell mass there is the obvious equivalent width upper limit, so when occurring that equivalent width is greater than the cell of the preset value of corresponding cell mass in cell to be measured, just abnormal cell can be identified.In one embodiment, when cell to be measured is leucocyte, and described leucocyte is divided at least three groups, its paracytic recognition methods is: if the equivalent width of the cell to be measured hived off in advance in lymphocyte populations exceedes lymphocytic preset value, be special-shaped abnormal lymphocytes by described cell recognition to be measured; If the equivalent width of the cell to be measured hived off in advance in granulocyte group exceedes granulocytic preset value, be immature granulocyte by described cell recognition to be measured.When leucocyte is divided into four groups or five groups, the abnormal cell in corresponding granulocyte group is just identified as the immature granulocyte of this group.
Identification module 750 can also obtain the second amplitude of the electric pulse reflecting cell complexity to be measured; Scatter diagram is generated according to the second amplitude and equivalent width; When equivalent width exceeds the preset value of corresponding cell mass, be identified as abnormal cell.When there is abnormal cell in cell mass, its equivalent width will exceed the preset value of corresponding cell mass, will be identified on scatter diagram very intuitively.
The invention also discloses a kind of blood cell analyzer, on the basis of existing blood cell analyzer, also comprise above-mentioned abnormal cell recognition system.Utilize the signal of reflection cell volume information to be measured to calculate the equivalent width of cell to be measured, in corresponding cell mass, identify abnormal cell.Do not need independent sense channel, thus reduce the complexity of instrument, make instrument miniaturization more.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (14)
1. a paracytic recognition methods, is characterized in that, comprises the following steps:
Cell to be measured is hived off in advance;
Obtain the signal of the described cell volume information to be measured of reflection and be converted into the first electric pulse, obtaining the first amplitude;
According to amplitude and the area of described first electric pulse, obtain the equivalent width of described first electric pulse;
The equivalent width of the cell to be measured in a cell mass and the preset value of described cell mass are compared;
If described equivalent width exceedes described preset value, be the abnormal cell in corresponding cell mass by described cell recognition to be measured.
2. paracytic recognition methods according to claim 1, is characterized in that, by the step that described cell to be measured hives off in advance is:
Obtain the signal of the described cell interior complexity information to be measured of reflection and be converted into the second electric pulse, obtaining the second amplitude;
According to described first amplitude and described second amplitude, described cell to be measured is hived off in advance.
3. paracytic recognition methods according to claim 2, it is characterized in that, the signal of the described cell interior complexity information to be measured of described reflection comprises lateral scattering light signal, forward direction high angle scatter light signal, fluorescence signal, radiofrequency signal, or the signal of described reflection cell volume information comprises forward-scattering signal or electrical impedance signal.
4. paracytic recognition methods according to claim 1, it is characterized in that, described cell to be measured is divided into lymphocyte, monocyte and granulocyte three groups by described hiving off in advance, or lymphocyte, monocyte, eosinophil and neutrophil leucocyte four groups, or lymphocyte, monocyte, basophilic granulocyte, eosinophil, neutrophil leucocyte five groups.
5. paracytic recognition methods according to claim 4, is characterized in that,
If the equivalent width of the cell to be measured hived off in advance in lymphocyte populations exceedes lymphocytic preset value, be special-shaped abnormal lymphocytes by described cell recognition to be measured; Or
If the equivalent width of the cell to be measured hived off in advance in granulocyte group exceedes granulocytic preset value, be immature granulocyte by described cell recognition to be measured.
6. paracytic recognition methods according to claim 1, is characterized in that, according to area and the amplitude of described electric pulse, the equivalent width obtaining described electric pulse specifically comprises the following steps:
Obtain the signal of the described cell volume to be measured of reflection and convert described signal to electric pulse;
Gather the amplitude of described electric pulse;
To described electric pulse integration, obtain integrated value, described integrated value represents the area of described electric pulse;
By the amplitude of the area of described electric pulse divided by described electric pulse, obtain the equivalent width of described electric pulse.
7. paracytic recognition methods according to claim 1, is characterized in that, before described equivalent width and preset value comparison step, also comprises by theory calculate or adds up Normocellular equivalent width, obtaining described preset value.
8. paracytic recognition methods according to claim 6, is characterized in that, reflects that the signal of described cell volume information to be measured is forward-scattering signal, the preferred forward direction of described forward-scattering signal low angle scattered light signal.
9. a paracytic recognition system, is characterized in that, comprising:
Pre-grouping module, hives off in advance by cell to be measured;
First amplitude module, obtains the signal of the described cell volume information to be measured of reflection and is converted into the first electric pulse, obtaining the first amplitude;
Equivalent width module, according to amplitude and the area of described first electric pulse, obtains the equivalent width of described first electric pulse;
Comparison module, compares the equivalent width of the cell to be measured in a cell mass and the preset value of described cell mass;
Described cell recognition to be measured, if described equivalent width exceedes described preset value, is the abnormal cell in corresponding cell mass by identification module.
10. paracytic recognition system according to claim 9, is characterized in that, described pre-grouping module comprises:
Second amplitude unit, obtains the signal of the described cell interior complexity information to be measured of reflection and is converted into the second electric pulse, obtaining the second amplitude;
Pre-point group unit, hives off described cell to be measured in advance according to described first amplitude and described second amplitude.
11. paracytic recognition systems according to claim 9, it is characterized in that, described cell to be measured is divided into lymphocyte, monocyte and granulocyte three groups by described pre-grouping module, or lymphocyte, monocyte, eosinophil and neutrophil leucocyte four groups, or lymphocyte, monocyte, basophilic granulocyte, eosinophil, neutrophil leucocyte five groups.
12. paracytic recognition systems according to claim 11, is characterized in that,
If the equivalent width of the cell to be measured hived off in advance in lymphocyte populations exceedes lymphocytic preset value, described cell recognition to be measured is special-shaped abnormal lymphocytes by identification module; Or
If the equivalent width of the cell to be measured hived off in advance in granulocyte group exceedes granulocytic preset value, described cell recognition to be measured is immature granulocyte by identification module.
13. paracytic recognition systems according to claim 9, is characterized in that, described equivalent width module comprises:
Acquiring unit, obtains the signal of the described cell volume to be measured of reflection and converts described signal to electric pulse;
Collecting unit, gathers the amplitude of described electric pulse;
Integral unit, to described electric pulse integration, obtains integrated value;
Computing unit, by described integrated value divided by amplitude, obtains the equivalent width of described electric pulse.
14. 1 kinds of blood cell analyzers, is characterized in that, comprise the paracytic recognition system in claim 9 to 13 described in any one.
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| CN107576634A (en) | 2018-01-12 |
| CN107576634B (en) | 2021-07-23 |
| CN104297213B (en) | 2017-10-03 |
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