CN104297135B - Recognizing method and recognizing system of particles in blood sample and blood cell analytic instrument - Google Patents
Recognizing method and recognizing system of particles in blood sample and blood cell analytic instrument Download PDFInfo
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- CN104297135B CN104297135B CN201310298758.3A CN201310298758A CN104297135B CN 104297135 B CN104297135 B CN 104297135B CN 201310298758 A CN201310298758 A CN 201310298758A CN 104297135 B CN104297135 B CN 104297135B
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Abstract
In the application, a recognizing method and a recognizing system of particles in a blood sample and a blood cell analytic instrument are disclosed. Firstly, according to an electronic pulse corresponding to a light scattering signal generated by irradiation on to-be-tested particles in the blood sample, a leukocyte group is recognized from the blood sample; and then an equivalent width indicating value of the to-be-tested particles in the blood sample is determined with correspondence to the electronic pulse by the light scattering signal which reflects size information of the to-be-tested particles; a scatter diagram of the equivalent width indicating value to electronic pulse amplitudes of information which reflects a complex degree of the to-be-tested particles is established; and non-cell particles are recognized from the leukocyte group in a manner of comparing the to-be-tested particles with a preset area in the scatter diagram. By means of the technical scheme, the non-cell particles in the blood sample can be recognized, thereby finally eliminating influence of the non-cell particles on leukocytes and improving recognizing accuracy of the particles in the blood sample.
Description
Technical field
The application is related to technical field of medical instruments, the recognition methods of particle, system in more particularly, to a kind of blood sample
And blood cell analyzer.
Background technology
Blood cell analyzer is a kind of instrument human blood cells being counted and being classified, and is widely used in facing
Bed and laboratory, mainly provide number of white blood cells（WBC）, mean constant of red blood cell（RBC）, mean corpuscular volume (MCV)（MCV）, blood platelet
Number（PLT）, mean platelet volume（MPV）, and the cell subset parameter of leucocyte, such as lymphocyte（LYM）Quantity or
Percentage, monocyte（MON）Quantity or percentage, neutrophil leucocyte（NEU）Quantity or percentage, eosinophil
（EOS）, basophilic granulocyte（BASO）The test parameter such as quantity or percentage.
Blood cell analyzer is typically classified to the leucocyte in blood sample using electrical impedance method or light scattering method
And counting.The General Principle of light scattering method is as follows：Blood sample preparatory unit by a certain amount of dilution after blood blood sample with
Reagent obtains sample liquid after having an effect, and sample liquid is transported to number system, and number system provides a flow chamber, flowing
Room provides an optical detection area, in this optical detection area, with sheath stream principle, sample liquid is wrapped in sheath stream
In, make the particle in sample liquid, such as acellular particles such as the fragment after leucocyte, erythrocyte hemolysis, lipid granule etc., lead to one by one
Cross optical detection area, number system also includes an optical system, this optical system provides LASER Light Source, produced light beam
It is irradiated on optical detection area, when particle flows through optical detection area, light beam is irradiated to generation light scattering on particle, counts
The detector of system is collected to the detection of scattered light in two scattered light angular ranges, scattered light can be converted to electric pulse defeated
Go out, finally, since corresponding between the amplitude of electric pulse and scattered light amplitude, formed with two scattered light amplitudes as reference axis
Double angle amplitude scatter diagrams.Because the inside complexity of different classes of particle and volume are all different, particle is produced because irradiating
In scattered light, high angular region（3 degree, to 30 degree, are numbered MAS）Scattered light amplitude reaction particle inside complexity, low angle model
Enclose（Less than 5 degree, it is numbered LAS）Scattered light amplitude reaction particle volume, thus according to grain on double angle amplitude scatter diagrams
The distribution situation of son, can distinguish different classes of particle.
Fig. 1 shows in leukocyte differential count（Differential, DIFF）Under passage, in blood sample, there is no lipid granule
Or in the case of other acellular particles, leucocyte and distribution feelings in above-mentioned pair of angle amplitude scatter diagram for the bib
Condition, Fig. 2 shows in basophilic granulocyte（BASO）Under passage, in the case of there is no acellular particle in blood sample, in vain carefully
Born of the same parents and distribution situation in above-mentioned pair of angle amplitude scatter diagram for the bib, as shown in Figure 1 or 2, leucocyte region with
Border between bib region is fully aware of, is therefore easy for distinguishing leucocyte and bib.Fig. 3 illustrates
Under DIFF passage, in the case of there is acellular particle in blood sample, leucocyte, acellular particle and bib
Distribution situation in above-mentioned pair of angle amplitude scatter diagram, Fig. 4 shows under BASO passage, there is acellular in blood sample
In the case of particle, leucocyte, the acellular particle and bib distribution situation in above-mentioned pair of angle amplitude scatter diagram,
As shown in Figure 3 or Figure 4, the adhesion part that overlaps that acellular particle region can be produced with leucocyte region, therefore, the meter of leucocyte
The accuracy of number and classification can be greatly affected, and the accuracy causing blood cell analyzer test result declines.
Content of the invention
The application provides the recognition methods of particle, system and blood cell analyzer in a kind of blood sample, to improve blood
The degree of accuracy of particle identification in liquid sample.
According to the application's in a first aspect, the application provides a kind of recognition methods of particle in blood sample, including：
Obtain blood sample；
Obtain and reflect in described blood sample the scattered light signal of particle volume information to be measured and be converted to the first electric pulse；
Obtain and reflect in described blood sample the scattered light signal of particle complexity information to be measured and be converted to the second electric arteries and veins
Punching；
According to the amplitude of described first electric pulse and the second electric pulse, identify leucocyte group from described blood sample；
Amplitude according to described first electric pulse and area, the equivalent width calculating particle to be measured in described leucocyte group refers to
Indicating value；
According to the amplitude of described second electric pulse, it is worth to scatter diagram with the instruction of described equivalent width；
Particle to be measured on described scatter diagram is compared with predeterminable area, identifies acellular particle.
According to the second aspect of the application, the application provides a kind of identifying system of particle in blood sample, including：
Electric pulse acquiring unit, is turned by the scattered light signal of particle volume information to be measured in reflection blood sample for obtaining
Change the first electric pulse of gained, and changed by the scattered light signal reflecting particle complexity information to be measured in described blood sample
Second electric pulse of gained；
First recognition unit, for the amplitude according to described first electric pulse and the second electric pulse, from described blood sample
In identify leucocyte group；
Second recognition unit, for the amplitude according to described first electric pulse and area, calculates in described leucocyte group and treats
Survey the equivalent width indicated value of particle；According to the amplitude of described second electric pulse, it is worth to scatterplot with the instruction of described equivalent width
Figure；Particle to be measured on described scatter diagram is compared with predeterminable area, identifies acellular particle.
According to the third aspect of the application, the application provides a kind of blood cell analyzer, including：
The identifying system of particle in blood sample described above；
And, number system, for counting to particle to be identified in described blood sample, obtain the described first electric arteries and veins
Punching and the second electric pulse.
The beneficial effect of the application is：
By providing the recognition methods of particle, system and blood cell analyzer in a kind of blood sample, it is first depending on blood
In liquid sample, particle to be measured, because irradiating the corresponding electric pulse of produced scattered light signal, identifies leucocyte from blood sample
Group, then determines particle to be identified in blood sample according to the corresponding electric pulse of the scattered light signal reflecting particle volume information to be measured
Equivalent width indicated value, set up equivalent width indicated value with reflect particle complexity information to be measured electric pulse amplitude scatterplot
Figure, by contrasting particle to be measured and predeterminable area on scatter diagram, thus identify acellular particle from leucocyte group.So,
Acellular particle in blood sample being identified, thus finally eliminating the impact to leucocyte for the acellular particle, carrying
The degree of accuracy of particle identification in high blood sample.
Brief description
Fig. 1 be prior art under DIFF passage formed do not interfere with particle blood sample corresponding with two dissipate
Penetrate double angle amplitude scatter diagrams that light amplitude is reference axis；
Fig. 2 be prior art under BASO passage formed do not interfere with particle blood sample corresponding with two dissipate
Penetrate double angle amplitude scatter diagrams that light amplitude is reference axis；
Fig. 3 be prior art under DIFF passage formed the blood sample containing acellular particle corresponding with two
Scattered light amplitude is double angle amplitude scatter diagrams of reference axis；
Fig. 4 be prior art under BASO passage formed the blood sample containing acellular particle corresponding with two
Scattered light amplitude is double angle amplitude scatter diagrams of reference axis；
Fig. 5 is the flow chart of the recognition methods of particle in the blood sample of the embodiment of the present application one；
Fig. 6 is using the amplitude of the equivalent width indicated value of particle and the second electric pulse as coordinate in the embodiment of the present application one
The width angle amplitude scatter diagram of the two dimension of axle；
Fig. 7 is the structure chart of the blood cell analyzer of the embodiment of the present application one；
Fig. 8 is the knot of the identifying system 703 of particle in blood sample in the blood cell analyzer of the embodiment of the present application one
Composition；
Fig. 9 is using the amplitude of the equivalent width indicated value of particle and the second electric pulse as coordinate in the embodiment of the present application two
The width angle amplitude scatter diagram of the two dimension of axle.
Specific embodiment
Combine accompanying drawing below by specific embodiment the application is described in further detail.
Embodiment one：
The main light scattering method classified with to the leucocyte in blood sample and counted of the present embodiment combines.
In addition it is also necessary to predefine and to store one pre- before the recognition methods of particle in the blood sample implementing the present embodiment
If region, this predeterminable area is by reference particle because the scattered light irradiating produced reaction reference particle complexity information is corresponding
The amplitude of the 4th electric pulse, and its equivalent width indicated value determines, predeterminable area can be reflected in following width angles jointly
In amplitude scatter diagram.Specifically, reference particle can be the simulation particle of leucocyte or leucocyte, goes out in blood cell analyzer
Before factory, select DIFF passage first on blood cell analyzer, using light scattering method, reference particle in reference sample is carried out
During counting, reference particle is because light irradiation produces the scattered light signal of reflection reference particle volume information and reacts reference particle again
Both scattered light signals can be accordingly converted to the 3rd electricity by photoelectric sensor by another scattered light signal of miscellaneous degree information
Pulse and the 4th electric pulse, and then the equivalent width instruction of reference particle according to the amplitude of the 3rd electric pulse and area, can be calculated
Value.The amplitude of electric pulse corresponding reflection scattered light amplitude.Equivalent width indicated value can be equivalent width L or equivalent
The 1/L reciprocal of width L, herein below is all illustrated with equivalent width indicated value for equivalent width L.Equivalent width L is the 3rd
The area of electric pulse and the ratio of amplitude, equivalent width L can be determined by the process step that following formula are reflected：Wherein, f (x) is electric pulse function, and x1 is the value lower limit of independent variable x in electric pulse function, x2
For the corresponding value upper limit, fmax is the amplitude of electric pulse, that is, the maximum of electric pulse function.Equivalent width L can be by such as
Lower handling process obtains：First, gather the amplitude of the 3rd electric pulse, then, the 3rd electric pulse is integrated, obtain characterizing the 3rd electricity
The integrated value of the area of pulse, then, by this integrated value divided by amplitude, obtains the equivalent width L of the 3rd electric pulse.Reflection reference
The scattered light signal of particle volume information is low angle scattered light, and the scattered light signal of reflection reference particle complexity information is the angle of elevation
Scattered light, wherein, high angular region is 3 degree to 30 degree, and low angular region is less than 5 degree.
After prestoring predeterminable area, you can carry out the identification of particle in the blood sample of the present embodiment under DIFF passage
Method, main inclusion flow process as shown in Figure 5：
Step 501, sample liquid preparation process.Specifically, add hemolytic agent with lysed erythrocyte in blood sample, obtain
Sample liquid, potentially includes multiple particles to be measured in this sample liquid, such as leucocyte and bib, or leucocyte, red blood cell
The acellular particle such as fragment and lipid particle that may be present.Wherein it should be noted that acellular particle is except red in blood
Particle outside the cells such as cell, leucocyte and blood platelet.
Step 502, when particle to be measured in blood sample being counted using light scattering method, is obtained in reflection blood sample
The scattered light signal of particle volume information to be measured is simultaneously converted to the first electric pulse, obtains particle to be measured in reflection blood sample complicated
The scattered light signal of degree information is simultaneously converted to the second electric pulse.Specifically, particle to be measured reflects particle to be measured because light irradiation produces
The scattered light signal of volume information, and reflect the scattered light signal of particle complexity information to be measured, by photoelectric sensor it is
Two kinds of scattered light signals accordingly can be converted to the first electric pulse and the second electric pulse.Reflect the scattering of particle volume information to be measured
Optical signal is low angle scattered light, reflects that the scattered light signal of particle complexity information to be measured is high angle scatter light, wherein, angle of elevation model
Enclose for 3 degree to 30 degree, low angular region is less than 5 degree.
Step 503, according to the amplitude of the first electric pulse and the second electric pulse, obtains double angle amplitude scatter diagrams.Specifically,
Amplitude according to the first electric pulse and the second electric pulse dissipates so that it may particle to be measured is mapped to double angle amplitudes as shown in Figure 3
Point in figure.
Step 504, identifies leucocyte group according to double angle amplitude scatter diagrams.Specifically, as shown in figure 3, red blood cell is broken
Piece corresponding region is perfectly clear with the border of leucocyte group corresponding region, therefore, can identify bib and be marked,
Also leucocyte group can be identified, this leucocyte group comprises leucocyte and acellular particle that may be present simultaneously.
Step 505, the amplitude according to the first electric pulse and area, the equivalent width calculating particle to be measured in leucocyte group refers to
Indicating value.Specifically, equivalent width indicated value is equivalent width L, and equivalent width L is the area of the first electric pulse and the ratio of amplitude
Value, equivalent width L can determine by above-mentioned formula, here is omitted.
Step 506, according to the amplitude of the second electric pulse, obtains the width angle of two dimension as shown in Figure 6 with equivalent width
Amplitude scatter diagram.
Step 507, the particle to be measured on scatter diagram is compared with predeterminable area, identifies acellular particle.Specifically,
Predeterminable area can have an identification boundary, and this identification boundary can behave as straight line or straight line in width angle amplitude scatter diagram
Proximal line.Relation according to particle to be measured on scatter diagram and identification boundary, you can identify acellular particle.As shown in fig. 6, should
Figure comprises the marked bib region 601 of step 504, and the leucocyte region 603 that separated by identification boundary 602 and
Acellular particle region 604, leucocyte region 603 is located at the right side of identification boundary 602, and acellular particle region 604 is located at
The left side of identification boundary 602.Particle label in leucocyte region 603 is leucocyte, by acellular particle region 604
Particle label is acellular particle, or the particle in acellular particle region 604 is marked with bib unification,
Thus acellular particle and leucocyte make a distinction the most at last.It is obvious that using the method for the present embodiment, finally eliminating non-thin
The impact to leucocyte for born of the same parents' particle, improves the degree of accuracy of particle identification in blood sample.
Further, due to acellular particle, the impact to leucocyte is excluded, then leukocyte marker can be more accurate,
So can be under DIFF passage, further with light scattering method, classification from marked leucocyte obtains lymphocyte
（LYM）, monocyte（MON）, neutrophil leucocyte（NEU）And eosinophil（EOS）Deng four subgroups, and obtain subgroup
Quantity or percentage.Because leucocyte is labeled, then can be under BASO passage, using light scattering method, from the leucocyte of mark
Middle classification obtains basophilic granulocyte（BASO）One subgroup, and obtain quantity or the percentage of this subgroup, and then acellular grain
The impact to WBC sub-population for the son is accordingly also excluded from, and leukocyte differential count is also more accurate.
Correspondingly, the blood cell analyzer of the present embodiment mainly includes structure as shown in Figure 7：Reaction tank 701, counting
System 702, and in blood sample particle identifying system 703.Wherein：
Reaction tank 701 is used for providing the reacting environment of blood sample and reagent, thus obtaining sample liquid.
Number system 702 is used for providing corresponding optical detection apparatus to complete to the sample coming from reaction tank 701 conveying
The light scattering method that liquid carries out particle in sample liquid counts, thus producing corresponding electric pulse.
In blood sample, the identifying system 703 of particle may include structure as shown in Figure 8：
Electric pulse acquiring unit 801, for obtaining by the scattered light letter of particle volume information to be measured in reflection blood sample
Number conversion gained the first electric pulse, and by reflection blood sample in particle complexity information to be measured scattered light signal conversion
Second electric pulse of gained.Specifically, in reflection blood sample, the scattered light signal of particle volume information to be measured is the scattering of low angle
Light, in reflection blood sample, the scattered light signal of particle complexity information to be measured is middle angle scattered light, and wherein, high angular region is 3
Spend to 30 degree, low angular region is less than 5 degree.
First recognition unit 802, for the amplitude according to the first electric pulse and the second electric pulse, from identifying leucocyte
Group.
Second recognition unit 803, for the amplitude according to the first electric pulse and area, calculates particle to be measured in leucocyte group
Equivalent width indicated value；According to the amplitude of the second electric pulse, it is worth to the width angle amplitude of two dimension with equivalent width instruction
Scatter diagram；Particle to be measured on width angle amplitude scatter diagram is compared with predeterminable area, identifies acellular particle.Specifically
Ground, equivalent width is designated as the area of the first electric pulse and the ratio of amplitude, equivalent width instruction can for above-mentioned equivalent width L or
The 1/L reciprocal of equivalent width L, here is omitted.Predeterminable area is the data prestoring, and this predeterminable area is by reference particle
Because irradiating the amplitude of corresponding 4th electric pulse of scattered light of produced reaction reference particle complexity information, and it is equivalent
Width indicated value determines jointly, and predeterminable area can be reflected in above-mentioned width angle amplitude scatter diagram.
In the present embodiment, blood sample is detected in the DIFF passage of blood cell analyzer.
Embodiment two：
The present embodiment is essentially consisted in embodiment one difference：
In the present embodiment, blood sample detected in the BASO passage of blood cell analyzer obtained by width angle
Degree amplitude scatter diagram is as shown in figure 9, this figure comprises leucocyte region 902 and the acellular particle area being separated by identification boundary 901
Domain 903, leucocyte region 901 is located at the right side of identification boundary 901, and acellular particle region 903 is located at identification boundary 901
Left side.
It should be noted that it is default under the predeterminable area under embodiment one DIFF passage with the present embodiment BASO passage
Region is different, thus predeterminable area corresponding identification boundary is also different.In addition, under BASO passage, due to bib phase
To for less, then can also select not carry out identification and the mark of bib.
Embodiment three：
The present embodiment is with any embodiment difference in embodiment one, two：
In the recognition methods of the present embodiment：Sample liquid can be transported in the number system of blood cell analyzer first
Flow chamber, make particle to be measured in sample liquid one by one through the optical detection area that flowing is indoor, when the light in optical system
When light beam produced by source is irradiated on particle to be measured the scattering of the concurrent third contact of a total solar or lunar eclipse, two detectors are by two seed light beams of two angles
Be converted to two electric pulses, by calculating the area of electric pulse and the ratio of amplitude, obtain the equivalent width instruction of particle to be measured
Value.And then formed between the amplitude three of equivalent width indicated value, the amplitude of the first electric pulse and the second electric pulse of particle
Corresponding relation, the width angle amplitude scatter diagram of the present embodiment is with the width of the equivalent width indicated value of particle, the first electric pulse
The amplitude of value and the second electric pulse is formed the scatter diagram of three-dimensional as reference axis, and by the particle to be measured on scatter diagram with pre-
If region compares, just can recognize that acellular particle.Wherein predeterminable area is determined by following manner：First, obtain instead
Reflect the scattered light signal of reference particle volume information, and the scattered light signal of reflection reference particle complexity information, and corresponding
Be converted to the 3rd electric pulse and the 4th electric pulse, reference particle is the simulation particle of leucocyte particle or leucocyte particle, then
Amplitude according to the 3rd electric pulse and area, calculate the equivalent width indicated value of reference particle, then, according to the 4th electric pulse
Amplitude determines predeterminable area with the equivalent width indicated value of reference particle in three-dimensional scatter diagram.So, in this predeterminable area
, then can recognize that as leucocyte and mark, outside this predeterminable area, then can recognize that as acellular particle.
Correspondingly, the second recognition unit 803 of the blood cell analyzer of the present embodiment, for according to the first electric pulse
Amplitude and area, calculate the equivalent width indicated value of particle to be measured in leucocyte group；According to the first electric pulse, second electric pulse
Amplitude, is worth to the width angle amplitude scatter diagram of three-dimensional with equivalent width instruction；By treating on width angle amplitude scatter diagram
Survey particle compared with predeterminable area, identify acellular particle.
Example IV：
The present embodiment is with any embodiment difference in embodiment one to three：Identification boundary dissipates in width angle amplitude
Point in figure shows as outside straight line or the proximal line of straight line, can also show as irregular obstacle body form such as curve etc..
Embodiment five：
The present embodiment is with any embodiment difference in embodiment one to five：Reference sample can be free from acellular
Particle or be substantially free of the blood of acellular particle through diluting gained sample.Or, reference sample can be only to comprise acellular
The sample of particle, or the simulation particle using acellular particle.
Above content is further description the application made with reference to specific embodiment it is impossible to assert this Shen
Being embodied as please is confined to these explanations.For the application person of an ordinary skill in the technical field, do not taking off
On the premise of the application design, some simple deduction or replace can also be made.
Claims (10)
1. in a kind of blood sample particle recognition methods it is characterised in that include：
Obtain blood sample；
Obtain and reflect in described blood sample the scattered light signal of particle volume information to be measured and be converted to the first electric pulse；
Obtain and reflect in described blood sample the scattered light signal of particle complexity information to be measured and be converted to the second electric pulse；
According to the amplitude of described first electric pulse and the second electric pulse, identify leucocyte group；
Amplitude according to described first electric pulse and area, calculate the equivalent width instruction of particle to be measured in described leucocyte group
Value, described equivalent width indicated value is the inverse of equivalent width or equivalent width, and described equivalent width is described first electric pulse
Area and amplitude ratio；
Amplitude according to described second electric pulse is worth to scatter diagram with described equivalent width instruction；
Particle to be measured on described scatter diagram is compared with predeterminable area, identifies acellular particle.
2. the method for claim 1 is it is characterised in that according to the amplitude of described first electric pulse and area, calculate institute
The equivalent width indicated value stating particle to be measured in leucocyte group includes：
Gather the amplitude of described first electric pulse；
To described first electric pulse integration, obtain integrated value, described integrated value characterizes the area of described first electric pulse；
By the area of described first electric pulse divided by the amplitude of described first electric pulse, obtain the equivalent width of described first electric pulse
Degree, obtains described equivalent width indicated value according to the equivalent width of described first electric pulse.
3. the method for claim 1 is it is characterised in that methods described also includes：
Obtain the scattered light signal of reflection reference particle volume information and be converted to the 3rd electric pulse, described reference particle is thin in vain
Born of the same parents, the simulation particle of the simulation particle of leucocyte, acellular particle or acellular particle；
Obtain the scattered light signal reflecting described reference particle complexity information and be converted to the 4th electric pulse；
Amplitude according to described 3rd electric pulse and area, calculate the equivalent width indicated value of described reference particle；
According to the amplitude of described 4th electric pulse, determine described predeterminable area with the equivalent width indicated value of described reference particle.
4. method as claimed any one in claims 1 to 3 is it is characterised in that to be measured in the described blood sample of described reflection
The scattered light signal of particle volume information is low angle scattered light signal.
5. method as claimed any one in claims 1 to 3 is it is characterised in that reflect particle to be measured in described blood sample
The scattered light signal of complexity information is high angle scatter optical signal.
6. in a kind of blood sample particle identifying system it is characterised in that include：
Electric pulse acquiring unit, for obtaining by the scattered light signal conversion institute of particle volume information to be measured in reflection blood sample
The first electric pulse obtaining, and gained is changed by the scattered light signal reflecting particle complexity information to be measured in described blood sample
The second electric pulse；
First recognition unit, for the amplitude according to described first electric pulse and the second electric pulse, knows from described blood sample
Do not go out leucocyte group；
Second recognition unit, for the amplitude according to described first electric pulse and area, calculates grain to be measured in described leucocyte group
The equivalent width indicated value of son, described equivalent width indicated value is the inverse of equivalent width or equivalent width, described equivalent width
Area for described first electric pulse and the ratio of amplitude；According to the amplitude of described second electric pulse, refer to described equivalent width
Indicating value obtains scatter diagram；Particle to be measured on described scatter diagram is compared with predeterminable area, identifies acellular particle.
7. system as claimed in claim 6 is it is characterised in that described second recognition unit includes：
Computation subunit, for gathering the amplitude of described first electric pulse；To described first electric pulse integration, obtain sign described
The integrated value of the area of the first electric pulse；By described integrated value divided by amplitude, obtain the equivalent width of described first electric pulse；
Identification subelement, for the amplitude according to described second electric pulse, obtains scatter diagram with described equivalent width；Dissipate described
Particle to be measured on point diagram, compared with predeterminable area, identifies acellular particle.
8. system as claimed in claims 6 or 7 is it is characterised in that reflect particle volume information to be measured in described blood sample
Scattered light signal be low angle scattered light signal.
9. system as claimed in claims 6 or 7 is it is characterised in that reflect particle complexity letter to be measured in described blood sample
The scattered light signal of breath is high angle scatter optical signal.
10. a kind of blood cell analyzer is it is characterised in that include：
The identifying system of particle in blood sample as any one of claim 6-9；
And, number system, for counting to particle to be identified in blood sample, obtain described first electric pulse and
Two electric pulses.
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| CN110226083B (en) | 2017-02-17 | 2022-12-20 | 深圳迈瑞生物医疗电子股份有限公司 | Erythrocyte fragment recognition method and device, blood cell analyzer and analysis method |
| CN116609243A (en) | 2018-04-28 | 2023-08-18 | 深圳迈瑞生物医疗电子股份有限公司 | Blood analysis method, blood analysis system, and storage medium |
| WO2019206311A1 (en) * | 2018-04-28 | 2019-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | Blood analysis system, blood analyzer, blood analysis method, and storage medium |
| WO2020133285A1 (en) * | 2018-12-28 | 2020-07-02 | 深圳迈瑞生物医疗电子股份有限公司 | Blood cell parameter correction method, blood sample detector and storage medium |
| CN114279941A (en) * | 2020-09-27 | 2022-04-05 | 深圳市帝迈生物技术有限公司 | Display method of scattered point image, sample analysis equipment and related device |
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