CN104293683A - Culture medium of antrodia cinnamomea mycelia - Google Patents
Culture medium of antrodia cinnamomea mycelia Download PDFInfo
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- CN104293683A CN104293683A CN201410536847.1A CN201410536847A CN104293683A CN 104293683 A CN104293683 A CN 104293683A CN 201410536847 A CN201410536847 A CN 201410536847A CN 104293683 A CN104293683 A CN 104293683A
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention relates to a culture medium of antrodia cinnamomea mycelia. The culture medium comprises the following components in percentage by weight: 25-50% of camphor tree leaves boiled liquid, 0.15-0.43% of camphor oil, 0.5-5% of a carbon source, 0.05-0.5% of a nitrogen source, 0.1-5% of yeast, 0.3-0.8% of an inorganic salt, 0.2-0.5% of a pH regulator, and the balance of secondary distilled water which is supplemented to 1000 ml. According to the invention, the culture medium is prepared by using the carbon source and the nitrogen source which are most suitable for the growth of antrodia cinnamomea and the camphor tree leaves boiled liquid in a certain weight ratio, and the pH of the culture medium is controlled to 6-7 so as to facilitate the fermentation and growth of antrodia cinnamomea mycelia; and the culture medium disclosed by the invention is simple in preparation method, and mature in the aspects of preservation and use technologies and the like, thereby facilitating the culturing and large-scale production of antrodia cinnamomea.
Description
Technical field
The present invention relates to a kind of fungi culture medium, be specifically related to the substratum of a kind of Cinnamomum kanahirai hay bacterium.
Background technology
Camphor tree sesame Antrodia camphorata is also known as Antrodia camphorata or Cinnamomum kanahirai hay mushroom, and camphor tree sesame is mainly distributed in the remote, thickly forested mountains of more than the 1000m above sea level such as the peach garden in Taiwan Province of China, southern destruction and Pingdong, and poor growth, output is rare.Research finds camphor tree sesame complicated component, containing a lot of physiologically active substance, there is multiple physiologically active, as anti-oxidant, protect the liver, anticancer, removing toxic substances, anti-inflammatory etc., to critical illnesss such as food poisonings, there is magical curative effect, Antrodia camphorata is also called as the ruby in forest in Taiwan, fill always " king of glossy ganoderma " good reputation.
In recent years, because the pharmacologically active of camphor tree sesame is day by day known to people, it eats or pharmaceutical use also highlights day by day, and the demand of people to camphor tree sesame also grows with each passing day.But wild camphor tree sesame rare numbers and obtain not easily, therefore, artificial culture method obtains camphor tree sesame becomes a kind of inevitable trend.The method of artificial culture camphor tree sesame comprises cultivation basswood method, liquid fermentation method etc., but these methods have the shortcomings such as culture cycle is long, toxigenic capacity is high, and the active constituent content such as the Antrodia Camphorata mycelium of turning out biology and triterpenes is low.For this reason, the invention provides a kind of Antrodia camphorata substratum, the preparation of this substratum is simple, preserves and operation technique maturation, and the various compositions needed for the growth of this substratum content Antrodia camphorata, the cultivation and the large-scale industrial fermentation that are suitable for Antrodia camphorata are produced.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the invention provides that a kind of preparation is simple, operation technique is ripe, be suitable for the substratum of the Cinnamomum kanahirai hay bacterium of large-scale production.
Technical scheme of the present invention is as follows:
A substratum for Cinnamomum kanahirai hay bacterium, described substratum comprises the component of following weight part:
Leaf of Cortex cinnamomi camphorae boiling liquid 25%-50%,
Fragrant camphor oil 0.15%-0.43%,
Carbon source 0.5%-5%,
Nitrogenous source 0.05%-0.5%,
Yeast 0.1%-5%,
Inorganic salt 0.3%-0.8%,
PH conditioning agent 0.2-0.5%,
Redistilled water complements to 1000ml.
Preferably, the concentration of described leaf of Cortex cinnamomi camphorae boiling liquid is 3%-5%.
Preferably, described leaf of Cortex cinnamomi camphorae boiling liquid is (3-5) by leaf of Cortex cinnamomi camphorae and water by volume: (97-95) boils 15-30min and obtain.
Preferably, described carbon source be selected from Semen Maydis powder, glucose or peptone one or more; Described nitrogenous source be selected from ammonium citrate, ammonium sulfate, wheat bran one or more; Described inorganic salt be selected from sodium-chlor, potassium hydrogen phosphate or potassium primary phosphate one or more.
Preferably, the weight ratio of described grape is 2%.
Preferably, described nitrogenous source is weight ratio is (1-3): the ammonium sulfate of (0.5-2) and the mixture of wheat bran.
Preferably, described nitrogenous source is the ammonium citrate of 0.2%.
Preferably, described PH conditioning agent is selected from the one in hydrochloric acid or sodium hydroxide.
Preferably, the PH of described substratum is 6-7.
A kind of preparation method of Antrodia camphorata substratum as above, it is characterized in that, comprise the following steps: by the fragrant camphor oil of 0.15%-0.43%, the carbon source of 0.5%-5%, the nitrogenous source of 0.05%-0.5%, the yeast of 0.1%-5%, the inorganic salt of 0.3%-0.8%, poach 15-30min, filter and remove residue, add the leaf of Cortex cinnamomi camphorae boiling liquid of 25%-50% in filtrate, mend redistilled water to 1000ml, after moist heat sterilization 20min, taking-up cooling is for subsequent use.
The present invention compared with prior art, beneficial effect be embodied in following some:
1. research display, the optimum condition that Antrodia Camphorata mycelium is cultivated is: PH is 6-7, the suitableeest carbon source is the glucose of 2%, the suitableeest nitrogenous source be 0.2% ammonium citrate or ammonium sulfate, wheat bran mixture, in the present invention, above-mentioned each component and leaf of Cortex cinnamomi camphorae boiling liquid and fragrant camphor oil are pressed the substratum that certain weight ratio forms camphor tree sesame bacterium, be beneficial to fermentation and the growth of Antrodia Camphorata mycelium.
2. research display, when utilizing Cortex cinnamomi camphorae sawdust medium to carry out the tentative cultivation of camphor tree sesame, mycelial growth is slow, and the liquid nutrient medium that the nutrition needed for growing containing camphor tree sesame is grown up is replaced traditional solid culture by the present invention, is more suitable for fermentation and the growth of Cinnamomum kanahirai hay bacterium.
3. substratum compound method of the present invention is simple, and the aspect such as preservation and operation technique is ripe, is beneficial to cultivation and the large-scale production of camphor tree sesame.
Embodiment
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1
A substratum for Cinnamomum kanahirai hay bacterium, described substratum comprises the component of following weight part:
Leaf of Cortex cinnamomi camphorae boiling liquid 25%,
Fragrant camphor oil 0.15%,
Carbon source 0.5%,
Nitrogenous source 0.05%
Yeast 0.1%,
Inorganic salt 0.3%,
PH conditioning agent 0.2%,
Redistilled water complements to 1000ml.
The concentration of described leaf of Cortex cinnamomi camphorae boiling liquid is 3%.
Described leaf of Cortex cinnamomi camphorae boiling liquid by leaf of Cortex cinnamomi camphorae and water by volume for 3:97 boils 15min and obtain.
Described carbon source is Semen Maydis powder; Described nitrogenous source is ammonium citrate; Described inorganic salt are sodium-chlor.
Described PH conditioning agent is hydrochloric acid.
The PH of described substratum is 6.
A kind of preparation method of Antrodia camphorata substratum as above, it is characterized in that, comprise the following steps: by the fragrant camphor oil of 0.15%, the glucose of 0.5%, the ammonium citrate of 0.05%, the yeast of 0.1%, the sodium-chlor of 0.3%, poach 15min, filter and remove residue, add the leaf of Cortex cinnamomi camphorae boiling liquid of 25% in filtrate, mend redistilled water to 1000ml, after moist heat sterilization 20min, taking-up cooling is for subsequent use.
Embodiment 2
A substratum for Cinnamomum kanahirai hay bacterium, described substratum comprises the component of following weight part:
Leaf of Cortex cinnamomi camphorae boiling liquid 50%,
Fragrant camphor oil 0.43%,
Carbon source 5%,
Nitrogenous source 0.5%,
Yeast 5%,
Inorganic salt 0.8%,
PH conditioning agent 0.5%,
Redistilled water complements to 1000ml.
The concentration of described leaf of Cortex cinnamomi camphorae boiling liquid is 5%.
Described leaf of Cortex cinnamomi camphorae boiling liquid by leaf of Cortex cinnamomi camphorae and water by volume for 5:95 boils 30min and obtain.
Described carbon source is peptone; Described nitrogenous source is ammonium sulfate; Described inorganic salt are potassium hydrogen phosphate.
Described PH conditioning agent is sodium hydroxide.
The PH of described substratum is 7.
A kind of preparation method of Antrodia camphorata substratum as above, it is characterized in that, comprise the following steps: by the fragrant camphor oil of 0.43%, the carbon source of 5 %, the nitrogenous source of 0.5%, the yeast of 5%, the inorganic salt of 0.8%, poach 30min, filter and remove residue, add the leaf of Cortex cinnamomi camphorae boiling liquid of 50% in filtrate, mend redistilled water to 1000ml, after moist heat sterilization 20min, taking-up cooling is for subsequent use.
Embodiment 3
A substratum for Cinnamomum kanahirai hay bacterium, described substratum comprises the component of following weight part:
Leaf of Cortex cinnamomi camphorae boiling liquid 35%,
Fragrant camphor oil 0.30%,
Carbon source 2%,
Nitrogenous source 0.25%,
Yeast 2.5%,
Inorganic salt 0.5%,
PH conditioning agent 0.3%,
Redistilled water complements to 1000ml.
The concentration of described leaf of Cortex cinnamomi camphorae boiling liquid is 4%.
Described leaf of Cortex cinnamomi camphorae boiling liquid by leaf of Cortex cinnamomi camphorae and water by volume for 4:96 boils 20min and obtain.
Described carbon source to be weight ratio be 2% glucose, described nitrogenous source to be weight ratio be 1:0.5 ammonium sulfate and the mixture of wheat bran.
Described PH conditioning agent is sodium hydroxide.
The PH of described substratum is 6.5.
A kind of preparation method of Antrodia camphorata substratum as above, it is characterized in that, comprise the following steps: by the fragrant camphor oil of 0.30%, the carbon source of 2%, the nitrogenous source of 0.25%, the yeast of 2.5%, the inorganic salt of 0.5%, poach 20min, filter and remove residue, add the leaf of Cortex cinnamomi camphorae boiling liquid of 35% in filtrate, mend redistilled water to 1000ml, after moist heat sterilization 20min, taking-up cooling is for subsequent use.
Above-described embodiment is only the preferred embodiment of the present invention, and should not be construed as limitation of the invention, and those skilled in the art, according to announcement of the present invention, do not depart from improvement that scope makes and amendment all should within protection scope of the present invention.
Claims (10)
1. a substratum for Cinnamomum kanahirai hay bacterium, is characterized in that, described substratum comprises the component of following weight part:
Leaf of Cortex cinnamomi camphorae boiling liquid 25%-50%,
Fragrant camphor oil 0.15%-0.43%,
Carbon source 0.5%-5%,
Nitrogenous source 0.05%-0.5%,
Yeast 0.1%-5%,
Inorganic salt 0.3%-0.8%,
PH conditioning agent 0.2-0.5%,
Redistilled water complements to 1000ml.
2. the substratum of Cinnamomum kanahirai hay bacterium according to claim 1, is characterized in that, the concentration of described leaf of Cortex cinnamomi camphorae boiling liquid is 3%-5%.
3. the substratum of Cinnamomum kanahirai hay bacterium according to claim 1, is characterized in that, described leaf of Cortex cinnamomi camphorae boiling liquid is (3-5) by leaf of Cortex cinnamomi camphorae and water by volume: (97-95) boils 15-30min and obtain.
4. the substratum of Cinnamomum kanahirai hay bacterium according to claim 1, is characterized in that, described carbon source be selected from Semen Maydis powder, glucose or peptone one or more; Described nitrogenous source be selected from ammonium citrate, ammonium sulfate, wheat bran one or more; Described inorganic salt be selected from sodium-chlor, potassium hydrogen phosphate or potassium primary phosphate one or more.
5. the substratum of Cinnamomum kanahirai hay bacterium according to claim 4, is characterized in that, the weight ratio of described grape is 2%.
6. the substratum of Cinnamomum kanahirai hay bacterium according to claim 4, is characterized in that, described nitrogenous source is weight ratio is (1-3): the ammonium sulfate of (0.5-2) and the mixture of wheat bran.
7. the substratum of Cinnamomum kanahirai hay bacterium according to claim 4, is characterized in that, described nitrogenous source is the ammonium citrate of 0.2%.
8. the substratum of Cinnamomum kanahirai hay bacterium according to claim 1, is characterized in that, described PH conditioning agent is selected from the one in hydrochloric acid or sodium hydroxide.
9. the substratum of Cinnamomum kanahirai hay bacterium according to claim 1, is characterized in that, the PH of described substratum is 6-7.
10. one kind as arbitrary in claim 1-8 as described in the preparation method of Antrodia camphorata substratum, it is characterized in that, comprise the following steps: by the fragrant camphor oil of 0.15%-0.43%, the carbon source of 0.5%-5%, the nitrogenous source of 0.05%-0.5%, the yeast of 0.1%-5%, the inorganic salt of 0.3%-0.8%, poach 15-30min, filter and remove residue, add the leaf of Cortex cinnamomi camphorae boiling liquid of 25%-50% in filtrate, mend redistilled water to 1000ml, after moist heat sterilization 20min, taking-up cooling is for subsequent use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410536847.1A CN104293683A (en) | 2014-10-13 | 2014-10-13 | Culture medium of antrodia cinnamomea mycelia |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410536847.1A CN104293683A (en) | 2014-10-13 | 2014-10-13 | Culture medium of antrodia cinnamomea mycelia |
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| CN104293683A true CN104293683A (en) | 2015-01-21 |
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| CN201410536847.1A Pending CN104293683A (en) | 2014-10-13 | 2014-10-13 | Culture medium of antrodia cinnamomea mycelia |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901611A (en) * | 2018-06-14 | 2018-11-30 | 广东食品药品职业学院 | A kind of Antrodia camphorata culture medium and its preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379082A (en) * | 2001-08-10 | 2002-11-13 | 吴丽玉 | Solid culture method of antrodia camphorate, its cultured solid substance, and its product and application |
| CN101191120A (en) * | 2006-11-28 | 2008-06-04 | 蔡达铭 | Culture method for antrodia cinnamomea |
| TW200835791A (en) * | 2007-02-26 | 2008-09-01 | Pei-Jung Li | Method for artificially propagating fruit body stroma of Antrodia Camphorate |
| CN103613430A (en) * | 2013-11-25 | 2014-03-05 | 浙江科技学院 | Antrodia cinnamomea growth medium and preparation method and application thereof |
-
2014
- 2014-10-13 CN CN201410536847.1A patent/CN104293683A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379082A (en) * | 2001-08-10 | 2002-11-13 | 吴丽玉 | Solid culture method of antrodia camphorate, its cultured solid substance, and its product and application |
| CN101191120A (en) * | 2006-11-28 | 2008-06-04 | 蔡达铭 | Culture method for antrodia cinnamomea |
| TW200835791A (en) * | 2007-02-26 | 2008-09-01 | Pei-Jung Li | Method for artificially propagating fruit body stroma of Antrodia Camphorate |
| CN103613430A (en) * | 2013-11-25 | 2014-03-05 | 浙江科技学院 | Antrodia cinnamomea growth medium and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 蔡博雅，等: "不同木屑培養基對牛樟芝生長之效應", 《中華林學季刊》 * |
| 陈清农 等: "樟芝宿主专一性之探讨", 《台湾农业化学与食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901611A (en) * | 2018-06-14 | 2018-11-30 | 广东食品药品职业学院 | A kind of Antrodia camphorata culture medium and its preparation method and application |
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