CN104292196B - One extracts ascorbic method from sweet tea - Google Patents
One extracts ascorbic method from sweet tea Download PDFInfo
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- CN104292196B CN104292196B CN201410544734.6A CN201410544734A CN104292196B CN 104292196 B CN104292196 B CN 104292196B CN 201410544734 A CN201410544734 A CN 201410544734A CN 104292196 B CN104292196 B CN 104292196B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
- C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D307/62—Three oxygen atoms, e.g. ascorbic acid
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Abstract
The present invention is open a kind of extracts ascorbic method from sweet tea, and its step comprises pre-treatment, extraction, filtration, centrifugal and dry.Present method extraction efficiency is high, and cost is low, is easy to suitability for industrialized production.
Description
Technical field
The invention belongs to technical field of agricultural product process, particularly relate to one and extract ascorbic method from sweet tea.
Background technology
Sweet tea, Rosaceae rubus, perennial wait-a-bit.Ye Tian is edible, is mainly distributed in Guangxi.Sweet tea, taste is sweet, cool in nature, flat, has heat-clearing, moistening lung, effect of relieve sore throat and expelling phlegm for arresting cough.Through Japanology find, sweet tea to pollen hypersensitivity and rhinitis, dermatitis etc. effective in cure.Sweet tea main component has Rubusoside, tea-polyphenol, total flavones, carbohydrate, protein, amino acid, abundant VITAMIN and mineral substance etc.Wherein, containing vitamins C in every 100g fresh tea leaf in its is 6406-11475mg.
Vitamins C (VC), i.e. L-AA, crystalline powder, odorless acid, is long placed in look and turns yellow, the unsaturated many hydroxy compounds containing 6 carbon, aobvious acid, and soluble in water and organic solvent is easily oxidized, in especially neutral and basic solution; Metal ion is as Cu
2+, Fe
2+etc. promoting that it is oxidized.In the environment of about pH5.5, vitamins C is more stable.Vitamins C not only has hypoglycemic, reducing blood-fat, beauty treatment, loses weight and prevent the effects such as scurvy, and can stop the formation of carcinogenic substance, drives away harmful free radicals in cell, avoids the damage of DNA molecular and promotes that human body is to the absorption of iron.Because human or animal body lacks the enzyme of synthesis xitix; can not self synthesise vitamins C; must obtain from food; therefore it is listed in the essential nutrient element of human or animal; not replaceable vital role is played, so it is widely used in pharmaceutical preparation, food-drink nutritional additive and animal-feed enhancer additives in protection human health and growth of animal process.
At present, extract ascorbic method and mainly contain: solvent-extraction process, ultrasound assisted extraction method and microbe fermentation method etc., but the subject matter that scale operation vitamins C faces is complex process, cost is high, and extraction efficiency is low.
Summary of the invention
The object of this invention is to provide one and extract ascorbic method from sweet tea, the problem that solve improves to extract ascorbic extraction efficiency, reduces extraction cost.Technical scheme provided by the present invention is:
One extracts ascorbic method from sweet tea, comprises the following steps:
(1) pre-treatment: dried sweet tea powder is broken to 20-200 order, obtains sweet tea powder.
(2) extract: be that 1:10-1:40 adds sweet tea powder and water respectively according to solid-to-liquid ratio, add buffered soln and adjust pH5.0-7.0, soak, then extract through extracting method and obtain sweet tea powder crude extract;
(3) filter: by gained sweet tea powder crude extract after filtration, then merging filtrate;
(4) centrifugal: by step (3) gained sweet tea powder extracting solution, after centrifugal, to get supernatant liquor;
(5) dry: by spray-dried for the supernatant liquor collected in step (4), the crude vitamin C powder namely obtained.
Sweet tea powder is broken to 20 orders in (1) by step.
Buffered soln described in step (2) is acetic acid or sodium acetate buffer.
The time of step (2) described immersion is 10-30min.
The method of step (2) described extraction is single microwave loss mechanisms, single enzyme-microwave―assisted extraction, microwave-ultrasonic in combination extraction method and microwave-ultrasonic-single enzyme assisted Extraction follow the example of in any one.
As preferably, described single enzyme is cellulase, and its consumption is 1-5%.
As preferably, described single microwave loss mechanisms, single enzyme-microwave―assisted extraction, microwave-ultrasonic in combination extraction method and microwave-ultrasonic-single enzyme assisted Extraction follow the example of in microwave power be 80-280W, extraction time 5-15min.
As preferably, described microwave-ultrasonic in combination extraction method and microwave-ultrasonic-single enzyme assisted Extraction follow the example of in ultrasonic power be 100-250W, extraction time 8-20min.
As preferably, centrifugal described in step (3), rotating speed is 10000r/min, centrifugation time 10min.
beneficial effect of the present invention
1, the extracting method that the present invention adopts effectively can shorten extraction time and improve extraction efficiency.
2, the present invention adopts water as extraction solution, adopts acetic acid and sodium-acetate as buffered soln, greatly reduces production cost, and can improve the extraction yield of VC;
Embodiment
To be easier to understand the present invention with reference to the following example, the embodiment provided does not limit the scope of the invention.
embodiment 1
Take the sweet tea powder end that 1g crosses 20 mesh sieves, add water with solid-to-liquid ratio 1:18, acetum regulates pH to be 5.0, after cold soaking 20min, 43 DEG C, adopts single microwave loss mechanisms, microwave power 200W, extracts 20min, repeats 4 times.Suction filtration merging filtrate, the centrifugal 10min of 10000r/min, collects supernatant liquor spraying dry and obtains ascorbic powder.After measured, the VC that the method obtains is 9.975mg, and productive rate reaches 90.8%.
embodiment 2
Take the sweet tea powder end that 1g crosses 20 mesh sieves, solid-to-liquid ratio 1:15 adds water, and sodium acetate buffer regulates pH5.0, after soaking 15min, adopts single enzyme-microwave―assisted extraction, adds 2% cellulase, and at 43 DEG C, microwave power 180W, extracts 18min, repeat 2 times.Suction filtration merging filtrate, the centrifugal 10min of 10000r/min, collects supernatant liquor spraying dry and obtains ascorbic powder.After measured, the VC that the method obtains is 10.075mg, and productive rate reaches 91.6%.
embodiment 3
Take the sweet tea powder end that 1g crosses 20 mesh sieves, add water with solid-to-liquid ratio 1:16, add acetum and regulate pH5.0, after soaking 10min, at 43 DEG C, adopt microwave-ultrasonic in combination extraction method, microwave power 150W, extract 12min, then, under ultrasonic power 200W, supersound extraction 16min, repeats 3 times.Suction filtration merging filtrate, the centrifugal 10min of 10000r/min, collects supernatant liquor spraying dry and obtains sweet tea VC powder.After measured, the method obtains Vitamin C content 10.46mg, and productive rate reaches 95.16%.
embodiment 4
Take the sweet tea powder end that 1g crosses 20 mesh sieves, solid-to-liquid ratio 1:14 adds water, regulates pH to be 5.0 with sodium acetate buffer, after soaking 10min, microwave-ultrasonic-single enzyme assisted Extraction is adopted to follow the example of, add 1.5% cellulase, then at 43 DEG C, under microwave power 120W, extract 10min, then, under ultrasonic power 180W, extract 16min, repeat 2 times.Suction filtration merging filtrate, the centrifugal 10min of 10000r/min, collects supernatant liquor spraying dry and obtains sweet tea vitamins C powder.After measured, the Vitamin C content 10.76mg that the method obtains, productive rate reaches 97.8%.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.
Claims (5)
1. from sweet tea, extract an ascorbic method, it is characterized in that, comprise the following steps:
(1) pre-treatment: dried sweet tea powder is broken to 20-200 order, obtains sweet tea powder.
(2) slightly extracting: be that 1:10-1:40 adds sweet tea powder and water respectively according to solid-to-liquid ratio, add buffered soln and adjust pH5.0-7.0, soak, then obtaining sweet tea powder crude extract through extracting;
(3) filter: by gained sweet tea powder crude extract after filtration, then merging filtrate;
(4) centrifugal: by step (3) gained sweet tea powder extracting solution, after centrifugal, to get supernatant liquor;
(5) dry: by spray-dried for the supernatant liquor collected in step (4), the crude vitamin C powder namely obtained;
Buffered soln described in step (2) is NaAc_HAc buffer solution;
The time of the immersion described in step (2) is 10-30min;
The method of the thick extraction described in step (2) is single microwave loss mechanisms, single enzyme-microwave―assisted extraction, microwave-ultrasonic in combination extraction method and microwave-ultrasonic-single enzyme assisted Extraction follow the example of in any one.
2. method according to claim 1, is characterized in that: dried sweet tea powder is broken to 20 orders in (1) by step.
3. method according to claim 1, is characterized in that: described single enzyme is cellulase, and its consumption is 1-5%.
4. method according to claim 1, it is characterized in that: described single microwave loss mechanisms, single enzyme-microwave―assisted extraction, microwave-ultrasonic in combination extraction method and microwave-ultrasonic-single enzyme assisted Extraction follow the example of in microwave power be 80-280W, extraction time 5-15min; Described microwave-ultrasonic in combination extraction method and microwave-ultrasonic-single enzyme assisted Extraction follow the example of in ultrasonic power be 100-250W, extraction time 8-20min.
5. method according to claim 1, is characterized in that: centrifugal described in step (4), and rotating speed is 10000r/min, centrifugation time 10min.
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| CN201410544734.6A CN104292196B (en) | 2014-10-15 | 2014-10-15 | One extracts ascorbic method from sweet tea |
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| CN201410544734.6A CN104292196B (en) | 2014-10-15 | 2014-10-15 | One extracts ascorbic method from sweet tea |
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| CN104292196B true CN104292196B (en) | 2016-01-20 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105061368A (en) * | 2015-08-06 | 2015-11-18 | 常州思宇环保材料科技有限公司 | Method for extracting vitamin C from rose |
| CN106432155B (en) * | 2016-09-27 | 2019-04-26 | 桂林茗兴生物科技有限公司 | One kind extracting ascorbic method from Chinese ephedra fruit |
| CN107056734A (en) * | 2017-04-28 | 2017-08-18 | 佘延英 | Ascorbic method is extracted from Sweet tea |
Citations (4)
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|---|---|---|---|---|
| CN101829209A (en) * | 2010-04-28 | 2010-09-15 | 广西大学 | Preparation method of fruit-flavored sweet tea polyphenols |
| CN103284148A (en) * | 2013-05-20 | 2013-09-11 | 国家***泡桐研究开发中心 | Processing method of persimmon leaf concentrated powder rich in natural vitamin C |
| CN103694205A (en) * | 2013-12-16 | 2014-04-02 | 小金县四姑娘山天然沙棘食品有限公司 | Method for extracting Vc from sea buckthorn |
| CN103815492A (en) * | 2014-03-18 | 2014-05-28 | 河北农业大学 | Processing method of high-vitamin C fresh jujube juice |
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2014
- 2014-10-15 CN CN201410544734.6A patent/CN104292196B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829209A (en) * | 2010-04-28 | 2010-09-15 | 广西大学 | Preparation method of fruit-flavored sweet tea polyphenols |
| CN103284148A (en) * | 2013-05-20 | 2013-09-11 | 国家***泡桐研究开发中心 | Processing method of persimmon leaf concentrated powder rich in natural vitamin C |
| CN103694205A (en) * | 2013-12-16 | 2014-04-02 | 小金县四姑娘山天然沙棘食品有限公司 | Method for extracting Vc from sea buckthorn |
| CN103815492A (en) * | 2014-03-18 | 2014-05-28 | 河北农业大学 | Processing method of high-vitamin C fresh jujube juice |
Non-Patent Citations (3)
| Title |
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| 微波提取高效液相色谱法测定花椰菜中的维生素C;吴海燕;《江苏农业科学》;20140425;第42卷(第4期);256-257 * |
| 柚子果肉中维生素C的超声波提取及高效液相色谱分析;李玉明;《计算机与应用化学》;20110528;第28卷(第5期);580-582 * |
| 甜茶的成分研究;徐位坤 等.;《广西植物》;19811231;第1卷(第4期);16 * |
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