CN104291886A - Composite organic fertilizer prepared from vegetable wastes and preparation method of composite organic fertilizer - Google Patents
Composite organic fertilizer prepared from vegetable wastes and preparation method of composite organic fertilizer Download PDFInfo
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- CN104291886A CN104291886A CN201410562990.8A CN201410562990A CN104291886A CN 104291886 A CN104291886 A CN 104291886A CN 201410562990 A CN201410562990 A CN 201410562990A CN 104291886 A CN104291886 A CN 104291886A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a composite organic fertilizer prepared from vegetable wastes. The composite organic fertilizer is a fermented product which is obtained by fermenting a composite microbes and the vegetable wastes, wherein the composite microbe is a mixture of bacillus mucilaginosus, bacillus subtilis, tiny bacillus, bacillus megatherium, pseudomonas aeruginosa, plant lactic acid bacteria and azotobacter chroococcum, the number ratio of all the strains is 1:(7-8):(4-6):(3-5):(1-3):(3-5):(1-2), and the total amount of viable bacteria is 2.0*10<9>-3.0*10<9> CFU/mL. The invention also discloses a preparation method of the composite organic fertilizer. The preparation method comprises the following steps: inoculating the microbes to prepare a culture medium; and adsorbing by using an adsorbent, and fermenting the vegetable wastes to obtain compost. The compost prepared by the preparation method disclosed by the invention can be used for promoting the growth development of plants, and reducing plant diseases and insect pests and reducing the use amount of the inorganic fertilizer, so that the vegetable wastes are effectively used, and pollution of wastes to farm environments is reduced.
Description
Technical field
The invention belongs to technical field of biological fertilizer, relate to a kind of compound organic fertilizer utilizing vegetable castoff to prepare, the invention still further relates to the preparation method of above-mentioned compound organic fertilizer.
Background technology
Along with the adjustment of Agriculture of China structure and improving constantly of agriculture production intensive degree, vegetables industry develops rapidly.But due to lower by practitioner's quality and that environmental consciousness is thin impact, the phenomenon of arbitrarily toppling over and piling up of a large amount of vegetables seedling bodies and residual sick sporocarp the like waste often can be seen in the vegetables production area of various places.These wastes all very easily rot to foul, not only the air soil in producing region and surface water body are polluted, also be easy to grow mosquitos and flies simultaneously, become bacillary or the propagating source of fungal disease, bring out succession crop morbidity, form vicious cycle, also cause the waste of a large amount of vegetable castoff simultaneously.
In addition, along with town dweller's growth in the living standard, the proportion that vegetable castoff occupies in Chinese MSW research is increasing, the surface runoff that the organism of these high-contents produces through rainfall, to wash away and underground leakage polluted surface water and underground water, greatly threaten water surrounding and human health safety.
At present, for the treatment process of vegetable castoff, be all generally directly be used as garbage bury or burning disposal, do not utilize the nutrients such as the N P and K of rich content in vegetable castoff.Cannot realize the resource recycling of vegetable castoff, be current problem demanding prompt solution.
Summary of the invention
The object of this invention is to provide a kind of compound organic fertilizer utilizing vegetable castoff to prepare, effectively utilize vegetable castoff resource, solve the problem that existing vegetable castoff is wasted.
Another object of the present invention is to provide the preparation method of above-mentioned compound organic fertilizer.
The technical solution adopted in the present invention is: a kind of compound organic fertilizer utilizing vegetable castoff to prepare, this compound organic fertilizer is the fermented product fermented by complex microorganism and vegetable castoff, complex microorganism is colloid bacillus cereus, subtilis, small genus bacillus, bacillus megaterium, Pseudomonas aeruginosa, plant lactobacillus, the mixture of azotobacter chroococcum, quantitative proportion between each bacterial classification closes: colloid bacillus cereus: subtilis: small genus bacillus: bacillus megaterium: Pseudomonas aeruginosa: plant lactobacillus: azotobacter chroococcum=1:7 ~ 8:4 ~ 6:3 ~ 5:1 ~ 3:3 ~ 5:1 ~ 2, total viable count is 2.0 × 10
9~ 3.0 × 10
9cFU/mL.
Another technical scheme of the present invention is: the preparation method of above-mentioned compound organic fertilizer, carries out according to following steps:
Step 1,
By Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium 1:3 ~ 5:2 by volume ~ 4:1 ~ 3 are inoculated on fermention medium, then carry out multiplication culture through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain mixed bacteria liquid A.
Step 2,
By colloid bacillus cereus, plant lactobacillus, azotobacter chroococcum is individually inoculated on fermention medium, multiplication culture is carried out again through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain colloid bacillus cereus nutrient solution, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution.
Step 3,
To process the colloid bacillus cereus nutrient solution obtained through step 2, plant lactobacillus nutrient solution, the mixed bacteria liquid A that azotobacter chroococcum nutrient solution and processing through step 1 obtains mixes according to volume ratio 1:3 ~ 5:1 ~ 3:18 ~ 22, obtains composite bacteria liquid.
Step 4,
The composite bacteria liquid obtained through step 3 is moved into absorption whipping device, sorbent material is dropped into absorption whipping device, stir, and make sorbent material keep loose not lump, obtain the sorbent material after adsorbing composite bacteria.
Step 5, vegetable castoff is crushed to particle diameter 3 ~ 5mm, extrude excessive moisture, control water content 60% ~ 70%, 0.1 ~ 0.5% the vegetable castoff after fragmentation is evenly sprinkled into by mass percentage by adsorbing the sorbent material after composite bacteria through step 4, adding mass percent is again 0.5 ~ 2 ‰ Paris white, be placed in reaction tank and carry out static compost fermentation, obtained.
The feature of another technical scheme of the present invention is also,
In step 1, the temperature of shaking table enlarged culturing is 28 ~ 33 DEG C, and pH value is 7.0 ~ 7.2, and incubation time is 16 ~ 24h; The culture temperature of seeding tank fermentation is 28 ~ 33 DEG C, and pH value is 6.8 ~ 7.2, and incubation time is 12 ~ 24h; Ferment tank culture temperature is 28 ~ 33 DEG C, and pH value is 6.8 ~ 7.2, and incubation time is 36 ~ 72h.
Fermention medium in step 1 and step 2 is made up of following raw material by mass percentage:
Sucrose 2% ~ 4%, W-Gum 2 ~ 4%, soybean cake powder 0.5 ~ 1.5%, NaCl0.3 ~ 0.5%, CaCO
30.01 ~ 0.03%, MgSO
47H
2o 0.02 ~ 0.04%, water 90% ~ 95%, the content summation of above component is 100%; The pH of described fermention medium is 7.0 ~ 7.5.
In step 2, colloid bacillus cereus and azotobacter chroococcum are aerobic cultivation, and shaking table enlarged culturing temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 16 ~ 24h; Seeding tank leavening temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 16 ~ 24h; Ferment tank temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 36 ~ 72h;
Plant lactobacillus is Anaerobic culturel, and shaking table enlarged culturing temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 20 ~ 24h; Seeding tank leavening temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 20 ~ 24h; Ferment tank temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 48 ~ 72h.
In step 4, sorbent material is one or more mixing of the peat composed of rotten mosses, gac, kaolin, diatomite, medical stone etc., and the particle diameter of sorbent material is 1 ~ 2mm, water content 35 ~ 40%, and absorption number of viable controls 2.0 × 10
8~ 3.0 × 10
8cFU/g.
In step 5, the concrete grammar of compost fermentation is, by covered rearing with plastic film on compost, turning every day once, controls heap body core temperature 53 DEG C ~ 57 DEG C, through 10 ~ 15 days, to vegetable castoff becomes thoroughly decomposed completely.
The invention has the beneficial effects as follows,
1). a kind of compound organic fertilizer utilizing vegetable castoff to prepare, by multiple useful to the plant material that microorganism and vegetable castoff produce, promote growing of plant, reduce the disease and pest of plant, reduce the usage quantity of inorganic fertilizer simultaneously, reduce production cost, reduce waste to the pollution of farm environment simultaneously, realize the recycle of resource and the upgrading synergy of agricultural-food.
2). a kind of preparation method of the compound organic fertilizer utilizing vegetable castoff to prepare, utilize a kind of by multiple can the bacteria preparation that is composited of the microorganism of symbiosis, by will activation multiple-microorganism and the common compost fermentation of vegetable castoff, be that plant can directly utilize the nutritive substances such as polysaccharide, monose, amino acid and mineral element by Substance Transformations such as the Mierocrystalline cellulose in vegetable castoff, starch, pectin and protein, effectively make use of the vegetables that vegetables production area goes out of use, avoid the corrupt contaminate environment of abandoned vegetable, save natural resources.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
A kind of compound organic fertilizer utilizing vegetable castoff to prepare of the present invention, this compound organic fertilizer is the fermented product fermented by complex microorganism and vegetable castoff, described complex microorganism is colloid bacillus cereus, subtilis, small genus bacillus, bacillus megaterium, Pseudomonas aeruginosa, plant lactobacillus, the mixture of azotobacter chroococcum, quantitative proportion between each bacterial classification closes: colloid bacillus cereus: subtilis: small genus bacillus: bacillus megaterium: Pseudomonas aeruginosa: plant lactobacillus: azotobacter chroococcum=1:7 ~ 8:4 ~ 6:3 ~ 5:1 ~ 3:3 ~ 5:1 ~ 2, total viable count is 2.0 × 10
9~ 3.0 × 10
9cFU/mL.
The preparation method of a kind of compound organic fertilizer utilizing vegetable castoff to prepare of the present invention, implements according to following steps:
Step 1,
By Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium 1:3 ~ 5:2 by volume ~ 4:1 ~ 3 are inoculated on fermention medium, multiplication culture is carried out again through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain mixed bacteria liquid A, wherein, the temperature of shaking table enlarged culturing is 28 ~ 33 DEG C, pH value is 7.0 ~ 7.2, and incubation time is 16 ~ 24h; The culture temperature of seeding tank fermentation is 28 ~ 33 DEG C, and pH value is 6.8 ~ 7.2, and incubation time is 12 ~ 24h; Ferment tank culture temperature is 28 ~ 33 DEG C, and pH value is 6.8 ~ 7.2, and incubation time is 36 ~ 72h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 2% ~ 4%, W-Gum 2 ~ 4%, soybean cake powder 0.5 ~ 1.5%, NaCl0.3 ~ 0.5%, CaCO
30.01 ~ 0.03%, MgSO
47H
2o 0.02 ~ 0.04%, water 90% ~ 95%, the content summation of above component is 100%; The pH of described fermention medium is 7.0 ~ 7.5.
Step 2,
By colloid bacillus cereus, plant lactobacillus, azotobacter chroococcum is individually inoculated on fermention medium, then carries out multiplication culture through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain colloid bacillus cereus nutrient solution, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution, wherein, colloid bacillus cereus and azotobacter chroococcum are aerobic cultivation, shaking table enlarged culturing temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 16 ~ 24h; Seeding tank leavening temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 16 ~ 24h; Ferment tank temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 36 ~ 72h;
Plant lactobacillus is Anaerobic culturel, and shaking table enlarged culturing temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 20 ~ 24h; Seeding tank leavening temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 20 ~ 24h; Ferment tank temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 48 ~ 72h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 2% ~ 4%, W-Gum 2 ~ 4%, soybean cake powder 0.5 ~ 1.5%, NaCl0.3 ~ 0.5%, CaCO
30.01 ~ 0.03%, MgSO
47H
2o 0.02 ~ 0.04%, water 90% ~ 95%, the content summation of above component is 100%; The pH of described fermention medium is 7.0 ~ 7.5.
Step 3,
To process the colloid bacillus cereus nutrient solution obtained through step 2, plant lactobacillus nutrient solution, the mixed bacteria liquid A that azotobacter chroococcum nutrient solution and processing through step 1 obtains mixes according to volume ratio 1:3 ~ 5:1 ~ 3:18 ~ 22, obtains composite bacteria liquid.
Step 4,
The composite bacteria liquid obtained through step 3 is moved into absorption whipping device, the sorbent material that one or more mixing by the peat composed of rotten mosses, gac, kaolin, diatomite, medical stone etc. form is dropped into absorption whipping device, stir, and make sorbent material keep loosely not luming, obtain the sorbent material after adsorbing composite bacteria, wherein, the particle diameter of sorbent material is 1 ~ 2mm, water content 35 ~ 40%, absorption number of viable controls 2.0 × 10
8~ 3.0 × 10
8cFU/g.
Step 5, vegetable castoff is crushed to particle diameter 3 ~ 5mm, extrude excessive moisture, control water content 60% ~ 70%, 0.1 ~ 0.5% the vegetable castoff after fragmentation is evenly sprinkled into by mass percentage by adsorbing the sorbent material after composite bacteria through step 4, adding mass percent is again 0.5 ~ 2 ‰ Paris white, be placed in reaction tank, by covered rearing with plastic film on compost, turning every day once, control heap body core temperature 53 DEG C ~ 57 DEG C, through 10 ~ 15 days, carry out static compost fermentation, to vegetable castoff becomes thoroughly decomposed completely, obtained.
The mechanism of action of the preparation method of a kind of compound organic fertilizer utilizing vegetable castoff to prepare of the present invention is as follows:
In step 1, adopt Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium co-inoculation, be because above-mentioned several microorganism is aerobic bacteria, and nutritional needs is single, growth rapidly, strong stress resistance, thus can realize multiple bacteria compound fermentation in liquid fermenting process; Adopt Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium press the volume ratio inoculation of 1:3 ~ 5:2 ~ 4:1 ~ 3, it is the difference according to each growth speed, make it in mixed fermentation process, keep suitable symbiosis ratio, and avoid producing obvious restraining effect each other.Wherein, Pseudomonas aeruginosa can produce the multiple enzyme such as chitinase, proteolytic enzyme, effectively can drive away the harmful organisms such as the root knot nematode in soil; Subtilis can produce the enzymes such as cellulase, proteolytic enzyme, amylase, mainly should be used for all kinds of organic matter of fermentation maturity, and its lipopeptid class active substance produced has obvious restraining effect to various plants soil-borne disease simultaneously; Small genus bacillus can produce the various bioactivators such as plant hormone, enzyme, flocculation agent, can improve crop disease-resistant, cold-resistant, drought-resistant ability; The organophosphorus that bacillus megaterium can effectively be degraded in soil.
In step 2, adopt colloid bacillus cereus, plant lactobacillus, azotobacter chroococcum is inoculated separately, be because above-mentioned three kinds of microorganisms are high for nutritional requirement, culture condition is comparatively strict, and the speed of growth and aerobic habit difference is greatly, must carry out pure culture separately.The potassium-bearing mineral of colloid bacillus cereus energy analysing silicon hydrochlorate and aluminosilicate composition, has molten phosphorus, releases potassium and fixed nitrogen function; Plant lactobacillus can promote that the organic matter decomposition in soil becomes soil ulmin, and can discharge the hormonal substance needed for plant-growth, can promote plant growth; Azotobacter chroococcum has stronger nitrogen fixing capacity, and can secrete growth hormone, promotes the growth of plant and the growth of fruit.
In step 3, step 1 and step 2 are cultivated the colloid bacillus cereus nutrient solution obtained, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution and mixed bacteria liquid A 1:3 ~ 5:1 by volume ~ 3:18 ~ 22 mix, be because this ratio be physiological habit according to each bacterial classification, the function undertaken in compost preparation more and fermentation standby after designed by the biomass difference that obtains, object is to ensure to promote to greatest extent that becoming thoroughly decomposed of vegetable castoff uses the rear beneficial effect to plant with it.
In step 4, adsorbing composite bacteria liquid with sorbing material, is to ensure that thalline in fermented liquid and active metabolite thereof can fully adhere on it, to transport and to preserve for a long time, adsorptive input amount is 0.1 ~ 0.5%, is because if input amount is excessive, high cost; Input amount is too low, and fermentation maturity speed is excessively slow.
In step 5, adding 0.5 ~ 2 ‰ Paris white, is the pH value in order to during fermentation regulate compost, and microorganism is bred under the acid or alkali environment of matters.
A kind of compound organic fertilizer utilizing vegetable castoff to prepare prepared by the present invention, after measured, except the nutritive substances such as the polysaccharide that can directly utilize containing plant, monose, amino acid and mineral element, simultaneously also containing to the helpful antimicrobial factors of plant-growth, plant hormone, VITAMIN isoreactivity material, immunizing power and the resistance against diseases of plant self can be significantly improved.In addition, these beneficial floras and adding of nutritive substance effectively can also improve edatope, promote soil fertility.
The using method of a kind of nettle compost of the present invention tea is:
Embodiment 1
By Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium by volume 1:3:2:1 are inoculated on fermention medium, multiplication culture is carried out again through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain mixed bacteria liquid A, wherein, the temperature of shaking table enlarged culturing is 28 DEG C, pH value is 7.0, and incubation time is 24h; The culture temperature of seeding tank fermentation is 28 DEG C, and pH value is 6.8, and incubation time is 24h; Ferment tank culture temperature is 28 DEG C, and pH value is 6.8, and incubation time is 72h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 4%, W-Gum 4%, soybean cake powder 1.43%, NaCl0.5%, CaCO
30.03%, MgSO
47H
2o 0.04%, water 90%, the content summation of above component is 100%; The pH of described fermention medium is 7.0.
By colloid bacillus cereus, plant lactobacillus, azotobacter chroococcum is individually inoculated on fermention medium, then carries out multiplication culture through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain colloid bacillus cereus nutrient solution, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution, wherein, colloid bacillus cereus and azotobacter chroococcum are aerobic cultivation, shaking table enlarged culturing temperature is 28 DEG C, and pH value is 6.9, and incubation time is 24h; Seeding tank leavening temperature is 28 DEG C, and pH value is 6.9, and incubation time is 24h; Ferment tank temperature is 28 DEG C, and pH value is 6.9, and incubation time is 72h;
Plant lactobacillus is Anaerobic culturel, and shaking table enlarged culturing temperature is 28 DEG C, and pH value is 6.9, and incubation time is 24h; Seeding tank leavening temperature is 28 DEG C, and pH value is 6.9, and incubation time is 24h; Ferment tank temperature is 28 DEG C, and pH value is 6.9, and incubation time is 72h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 4%, W-Gum 4%, soybean cake powder 1.43%, NaCl 0.5%, CaCO
30.03%, MgSO
47H
2o 0.04%, water 90%, the content summation of above component is 100%; The pH of described fermention medium is 7.0.
By the colloid bacillus cereus nutrient solution obtained, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution and mixed bacteria liquid A, according to volume ratio 1:3:1:18 mixing, obtain composite bacteria liquid.The composite bacteria liquid obtained is moved into absorption whipping device, the mixed adsorbent of gac and kaolin composition is dropped into absorption whipping device, stir, and make mixed adsorbent keep loosely not luming, obtain the mixed adsorbent after adsorbing composite bacteria, wherein, the particle diameter of mixed adsorbent is 1mm, water content 35%, absorption number of viable controls 2.0 × 10
8cFU/g.Vegetable castoff is crushed to particle diameter 3mm, extrudes excessive moisture, control water content 60%, then the mixed adsorbent after absorption composite bacteria 0.1% is evenly sprinkled into the vegetable castoff after fragmentation by mass percentage, adding mass percent is again 0.5 ‰ Paris whites, is placed in reaction tank, by covered rearing with plastic film on compost, turning every day once, control heap body core temperature 53 DEG C, through 15 days, carry out static compost fermentation, to vegetable castoff becomes thoroughly decomposed completely, obtained.
Embodiment 2
By Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium by volume 1:4:3:2 are inoculated on fermention medium, multiplication culture is carried out again through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain mixed bacteria liquid A, wherein, the temperature of shaking table enlarged culturing is 29 DEG C, pH value is 7.1, and incubation time is 22h; The culture temperature of seeding tank fermentation is 29 DEG C, and pH value is 7.0, and incubation time is 20h; Ferment tank culture temperature is 29 DEG C, and pH value is 7.0, and incubation time is 60h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 2%, W-Gum 2%, soybean cake powder 0.67%, NaCl0.3%, CaCO
30.01%, MgSO
47H
2o 0.02%, water 95%, the content summation of above component is 100%; The pH of described fermention medium is 7.2.
By colloid bacillus cereus, plant lactobacillus, azotobacter chroococcum is individually inoculated on fermention medium, then carries out multiplication culture through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain colloid bacillus cereus nutrient solution, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution, wherein, colloid bacillus cereus and azotobacter chroococcum are aerobic cultivation, shaking table enlarged culturing temperature is 29 DEG C, and pH value is 7.0, and incubation time is 22h; Seeding tank leavening temperature is 29 DEG C, and pH value is 7.0, and incubation time is 22h; Ferment tank temperature is 29 DEG C, and pH value is 7.0, and incubation time is 60h;
Plant lactobacillus is Anaerobic culturel, and shaking table enlarged culturing temperature is 29 DEG C, and pH value is 7.0, and incubation time is 22h; Seeding tank leavening temperature is 29 DEG C, and pH value is 7.0, and incubation time is 22h; Ferment tank temperature is 29 DEG C, and pH value is 7.0, and incubation time is 65h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 2%, W-Gum 2%, soybean cake powder 0.67%, NaCl0.3%, CaCO
30.01%, MgSO
47H
2o 0.02%, water 95%, the content summation of above component is 100%; The pH of described fermention medium is 7.2.
By the colloid bacillus cereus nutrient solution obtained, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution and mixed bacteria liquid A, according to volume ratio 1:4:2:19 mixing, obtain composite bacteria liquid.The composite bacteria liquid obtained is moved into absorption whipping device, the mixed adsorbent of the peat composed of rotten mosses and kaolin composition is dropped into absorption whipping device, stir, and make mixed adsorbent keep loosely not luming, obtain the mixed adsorbent after adsorbing composite bacteria, wherein, the particle diameter of mixed adsorbent is 1.3mm, water content 37%, absorption number of viable controls 2.3 × 10
8cFU/g.Vegetable castoff is crushed to particle diameter 3.5mm, extrudes excessive moisture, control water content 63%, then the mixed adsorbent after absorption composite bacteria 0.2% is evenly sprinkled into the vegetable castoff after fragmentation by mass percentage, adding mass percent is again 1 ‰ Paris whites, is placed in reaction tank, by covered rearing with plastic film on compost, turning every day once, control heap body core temperature 55 DEG C, through 13 days, carry out static compost fermentation, to vegetable castoff becomes thoroughly decomposed completely, obtained.
Embodiment 3
By Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium by volume 1:5:4:3 are inoculated on fermention medium, multiplication culture is carried out again through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain mixed bacteria liquid A, wherein, the temperature of shaking table enlarged culturing is 31 DEG C, pH value is 7.1, and incubation time is 19h; The culture temperature of seeding tank fermentation is 31 DEG C, and pH value is 7.1, and incubation time is 16h; Ferment tank culture temperature is 31 DEG C, and pH value is 7.0, and incubation time is 48h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 3%, W-Gum 3%, soybean cake powder 1.5%, NaCl0.4%, CaCO
30.02%, MgSO
47H
2o 0.03%, water 92.05%, the content summation of above component is 100%; The pH of described fermention medium is 7.4.
By colloid bacillus cereus, plant lactobacillus, azotobacter chroococcum is individually inoculated on fermention medium, then carries out multiplication culture through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain colloid bacillus cereus nutrient solution, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution, wherein, colloid bacillus cereus and azotobacter chroococcum are aerobic cultivation, shaking table enlarged culturing temperature is 31 DEG C, and pH value is 7.1, and incubation time is 19h; Seeding tank leavening temperature is 31 DEG C, and pH value is 7.1, and incubation time is 19h; Ferment tank temperature is 31 DEG C, and pH value is 7.1, and incubation time is 48h;
Plant lactobacillus is Anaerobic culturel, and shaking table enlarged culturing temperature is 31 DEG C, and pH value is 7.1, and incubation time is 21h; Seeding tank leavening temperature is 31 DEG C, and pH value is 7.1, and incubation time is 21h; Ferment tank temperature is 31 DEG C, and pH value is 7.1, and incubation time is 55h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 3%, W-Gum 3%, soybean cake powder 1.5%, NaCl0.4%, CaCO
30.02%, MgSO
47H
2o 0.03%, water 92.05%, the content summation of above component is 100%; The pH of described fermention medium is 7.4.
By the colloid bacillus cereus nutrient solution obtained, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution and mixed bacteria liquid A, according to volume ratio 1:5:3:20 mixing, obtain composite bacteria liquid.The composite bacteria liquid obtained is moved into absorption whipping device, the mixed adsorbent of diatomite and medical stone composition is dropped into absorption whipping device, stir, and make mixed adsorbent keep loosely not luming, obtain the mixed adsorbent after adsorbing composite bacteria, wherein, the particle diameter of mixed adsorbent is 1.7mm, water content 39%, absorption number of viable controls 2.7 × 10
8cFU/g.Vegetable castoff is crushed to particle diameter 4mm, extrudes excessive moisture, control water content 67%, then the mixed adsorbent after absorption composite bacteria 0.4% is evenly sprinkled into the vegetable castoff after fragmentation by mass percentage, adding mass percent is again 1.5 ‰ Paris whites, is placed in reaction tank, by covered rearing with plastic film on compost, turning every day once, control heap body core temperature 56 DEG C, through 12 days, carry out static compost fermentation, to vegetable castoff becomes thoroughly decomposed completely, obtained.
Embodiment 4
By Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium by volume 1:4:2:3 are inoculated on fermention medium, multiplication culture is carried out again through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain mixed bacteria liquid A, wherein, the temperature of shaking table enlarged culturing is 33 DEG C, pH value is 7.2, and incubation time is 16h; The culture temperature of seeding tank fermentation is 33 DEG C, and pH value is 7.2, and incubation time is 12h; Ferment tank culture temperature is 33 DEG C, and pH value is 7.2, and incubation time is 36h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 4%, W-Gum 2%, soybean cake powder 0.5%, NaCl0.4%, CaCO
30.03%, MgSO
47H
2o 0.02%, water 93.05%, the content summation of above component is 100%; The pH of described fermention medium is 7.5.
By colloid bacillus cereus, plant lactobacillus, azotobacter chroococcum is individually inoculated on fermention medium, then carries out multiplication culture through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain colloid bacillus cereus nutrient solution, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution, wherein, colloid bacillus cereus and azotobacter chroococcum are aerobic cultivation, shaking table enlarged culturing temperature is 33 DEG C, and pH value is 7.2, and incubation time is 16h; Seeding tank leavening temperature is 33 DEG C, and pH value is 7.2, and incubation time is 16h; Ferment tank temperature is 33 DEG C, and pH value is 7.2, and incubation time is 36h;
Plant lactobacillus is Anaerobic culturel, and shaking table enlarged culturing temperature is 33 DEG C, and pH value is 7.2, and incubation time is 20h; Seeding tank leavening temperature is 33 DEG C, and pH value is 7.2, and incubation time is 20h; Ferment tank temperature is 33 DEG C, and pH value is 7.2, and incubation time is 48h.Fermention medium is made up of following raw material by mass percentage:
Sucrose 4%, W-Gum 2%, soybean cake powder 0.5%, NaCl0.4%, CaCO
30.03%, MgSO
47H
2o 0.02%, water 93.05%, the content summation of above component is 100%; The pH of described fermention medium is 7.5.
By the colloid bacillus cereus nutrient solution obtained, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution and mixed bacteria liquid A, according to volume ratio 1:4:3:22 mixing, obtain composite bacteria liquid.The composite bacteria liquid obtained is moved into absorption whipping device, peat composed of rotten mosses sorbent material is dropped into absorption whipping device, stir, and make sorbent material keep loosely not luming, obtain the sorbent material after adsorbing composite bacteria, wherein, the particle diameter of sorbent material is 2mm, water content 40%, absorption number of viable controls 3.0 × 10
8cFU/g.Vegetable castoff is crushed to particle diameter 5mm, extrudes excessive moisture, control water content 70%, then the sorbent material after absorption composite bacteria 0.5% is evenly sprinkled into the vegetable castoff after fragmentation by mass percentage, adding mass percent is again 2.0 ‰ Paris whites, is placed in reaction tank, by covered rearing with plastic film on compost, turning every day once, control heap body core temperature 57 DEG C, through 10 days, carry out static compost fermentation, to vegetable castoff becomes thoroughly decomposed completely, obtained.
The cucumber Tanaka of vegetable waste organic fertilizer to Pang Guangzhen Si Fu village, Hu County, Shaanxi that Example 1 obtains uses the rear experiment to cucumber growth-promoting effect, after measured, the examination soil organism is supplied to be 8.6g/kg, full nitrogen 0.88g/kg, rapid available phosphorus 68.7mg/kg, available potassium 116.2mg/kg, pH value 8.6, continuous plantation cucumber 5 years, kind is that Tianjin grinds No. 1.Experiment establishes 3 process, every community two row, and repeat for three times, random district group arranges.Process 1: use by the fertilizer prepared by embodiment 1 method, consumption is 750kg/hm
2; Process 2: use fertile special strange board vegetables compound fertilizer special (production of Shandong Li Nongfei industry company limited), consumption is 750kg/hm
2; Process 3 is blank.All adopt five point samplings to each process community, determine strain at often and investigate 2 strains, to the plant height of every strain, stem diameter, leaf length, Ye Kuan, petiole is long, the first female flower internode is long, fruit bar is long, fruit diameter and single fruit weight are investigated.
Experimental result is as shown in table 1, compared with commercially available chemical compound fertilizer product, the vegetable waste organic fertilizer utilizing the embodiment of the present invention 1 to prepare for cucumber fruit bar length, fruit diameter, mean fruit weight, pulp thickness comparatively contrasts all higher rate of increase, illustrates there is obvious production-increasing function to cucumber.
Table 1 utilizes vegetable waste organic fertilizer prepared by the embodiment of the present invention 1 to cucumber growth-promoting effect
The obtained vegetable waste organic fertilizer of Example 2 is to using the rear experiment to capsicum and tomato growth-promoting effect in the booth in Zhong Fengdian village, sluice gate town, Changan District, Xi'an, after measured, the examination soil organism is supplied to be 9.6g/kg, full nitrogen 0.92g/kg, rapid available phosphorus 74.7mg/kg, available potassium 126.8mg/kg, pH value 8.4, continuous plantation tomato and capsicum 6 years, kind is respectively middle vegetables No. 4 and Jiyan No.4.Experiment establishes 3 process respectively to two kinds of vegetables, every community two row, and repeat for three times, random district group arranges.Process 1: use by the fertilizer prepared by embodiment 2 method, consumption is 750kg/hm
2; Process 2: use fertile special strange board vegetables compound fertilizer special (production of Shandong Li Nongfei industry company limited), consumption is 750kg/hm
2; Process 3 is blank.Five point samplings are all adopted to each process community, determines strain at often and investigate 2 strains, observe its Other Main Agronomic Characters respectively, add up the incidence of its blight and root knot nematode, measure each cell production respectively collection period.
Experimental result is as shown in table 2, compared with commercially available chemical compound fertilizer product, the vegetable waste organic fertilizer utilizing the present invention to prepare, for the generation Be very effective preventing and treating root knot nematode and soil-borne disease in tomato, pepper cultivation process, also has obvious effect of increasing production simultaneously.
The facilitation effect that table 2 different fertilization is produced tomato and capsicum
Claims (7)
1. the compound organic fertilizer utilizing vegetable castoff to prepare, it is characterized in that, compound organic fertilizer is the fermented product fermented by complex microorganism and vegetable castoff, described complex microorganism is colloid bacillus cereus, subtilis, small genus bacillus, bacillus megaterium, Pseudomonas aeruginosa, plant lactobacillus, the mixture of azotobacter chroococcum, quantitative proportion between each bacterial classification closes: colloid bacillus cereus: subtilis: small genus bacillus: bacillus megaterium: Pseudomonas aeruginosa: plant lactobacillus: azotobacter chroococcum=1:7 ~ 8:4 ~ 6:3 ~ 5:1 ~ 3:3 ~ 5:1 ~ 2, total viable count is 2.0 × 10
9~ 3.0 × 10
9cFU/mL.
2. utilize vegetable castoff to prepare a method for compound organic fertilizer, it is characterized in that, specifically implement according to following steps:
Step 1,
By Pseudomonas aeruginosa, subtilis, small genus bacillus and bacillus megaterium 1:3 ~ 5:2 by volume ~ 4:1 ~ 3 are inoculated on fermention medium, then carry out multiplication culture through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain mixed bacteria liquid A;
Step 2,
By colloid bacillus cereus, plant lactobacillus, azotobacter chroococcum is individually inoculated on fermention medium, multiplication culture is carried out again through shaking table enlarged culturing, seeding tank fermentation and ferment tank, obtain colloid bacillus cereus nutrient solution, plant lactobacillus nutrient solution, azotobacter chroococcum nutrient solution;
Step 3,
To process the colloid bacillus cereus nutrient solution obtained through step 2, plant lactobacillus nutrient solution, the mixed bacteria liquid A that azotobacter chroococcum nutrient solution and processing through step 1 obtains mixes according to volume ratio 1:3 ~ 5:1 ~ 3:18 ~ 22, obtains composite bacteria liquid;
Step 4,
The composite bacteria liquid obtained through step 3 is moved into absorption whipping device, sorbent material is dropped into absorption whipping device, stir, and make sorbent material keep loose not lump, obtain the sorbent material after adsorbing composite bacteria;
Step 5, vegetable castoff is crushed to particle diameter 3 ~ 5mm, extrude excessive moisture, control water content 60% ~ 70%, 0.1 ~ 0.5% the vegetable castoff after fragmentation is evenly sprinkled into by mass percentage by adsorbing the sorbent material after composite bacteria through step 4, adding mass percent is again 0.5 ~ 2 ‰ Paris white, be placed in reaction tank and carry out static compost fermentation, obtained.
3. a kind of method utilizing vegetable castoff to prepare compound organic fertilizer according to claim 2, is characterized in that, in described step 1, the temperature of shaking table enlarged culturing is 28 ~ 33 DEG C, and pH value is 7.0 ~ 7.2, and incubation time is 16 ~ 24h; The culture temperature of seeding tank fermentation is 28 ~ 33 DEG C, and pH value is 6.8 ~ 7.2, and incubation time is 12 ~ 24h; Ferment tank culture temperature is 28 ~ 33 DEG C, and pH value is 6.8 ~ 7.2, and incubation time is 36 ~ 72h.
4. a kind of method utilizing vegetable castoff to prepare compound organic fertilizer according to claim 2, it is characterized in that, fermention medium in described step 1 and step 2 is made up of following raw material by mass percentage: sucrose 2% ~ 4%, W-Gum 2 ~ 4%, soybean cake powder 0.5 ~ 1.5%, NaCl0.3 ~ 0.5%, CaCO
30.01 ~ 0.03%, MgSO
47H
2o0.02 ~ 0.04%, water 90% ~ 95%, the content summation of above component is 100%; The pH of described fermention medium is 7.0 ~ 7.5.
5. a kind of method utilizing vegetable castoff to prepare compound organic fertilizer according to claim 2, is characterized in that, in described step 2, colloid bacillus cereus and azotobacter chroococcum are aerobic cultivation, shaking table enlarged culturing temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 16 ~ 24h; Seeding tank leavening temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 16 ~ 24h; Ferment tank temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 36 ~ 72h;
Plant lactobacillus is Anaerobic culturel, and shaking table enlarged culturing temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 20 ~ 24h; Seeding tank leavening temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 20 ~ 24h; Ferment tank temperature is 28 ~ 33 DEG C, and pH value is 6.9 ~ 7.2, and incubation time is 48 ~ 72h.
6. a kind of method utilizing vegetable castoff to prepare compound organic fertilizer according to claim 2, it is characterized in that, in described step 4, described sorbent material is one or more mixing of the peat composed of rotten mosses, gac, kaolin, diatomite, medical stone, the particle diameter of sorbent material is 1 ~ 2mm, water content 35 ~ 40%, absorption number of viable controls 2.0 × 10
8~ 3.0 × 10
8cFU/g.
7. a kind of method utilizing vegetable castoff to prepare compound organic fertilizer according to claim 2, it is characterized in that, in described step 5, the concrete grammar of compost fermentation is, by covered rearing with plastic film on compost, turning every day once, controls heap body core temperature 53 DEG C ~ 57 DEG C, through 10 ~ 15 days, to vegetable castoff becomes thoroughly decomposed completely.
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| CN116751721A (en) * | 2023-07-18 | 2023-09-15 | 陕西省微生物研究所 | Lactobacillus plantarum 121-5 and application thereof in preparing resveratrol by converting polydatin |
| CN116751721B (en) * | 2023-07-18 | 2024-02-13 | 陕西省微生物研究所 | Lactobacillus plantarum 121-5 and application thereof in preparing resveratrol by converting polydatin |
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