CN104288214B - A kind of hay-scented composition of medicine, preparation method and applications - Google Patents
A kind of hay-scented composition of medicine, preparation method and applications Download PDFInfo
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Abstract
The invention provides a kind of hay-scented composition of medicine, the composition of medicine is made up of the extract of Chinese artichoke, the Radix Astragali, red ginseng and the banksia rose.Composition of medicine provided by the invention, there is good activity to treatment diarrhoea, a variety of formulations can be developed into.
Description
Technical field
The present invention relates to a kind of Chinese medical extract prescription, more specifically to a kind of hay-scented composition of medicine, preparation method
And its application.
Technical background
Stachyose is that naturally occurring in Labiatae betony to remarkably promote human body intestinal canal profitable strain value-added
Functional oligose, it is described as " natural super bifidobacterium ".Regard as GRAS's (Generally Recognized as safe foodstuffs without toxicity) by U.S. FAD
Oligomeric sugar product.The eighties in last century, Japanese begin to study the function of stachyose.Stachyose is low with other features
Glycan is similar, and it is not decomposed by human body gastrointestinal disturbances liquid, can be beneficial by the Bifidobacterium in human body intestinal canal, Bacillus acidi lactici etc.
Flora is utilized, and promotes the increment of these beneficial bacteriums.In addition, stachyose can also suppress spoilage organisms, such as EHEC and aerogenesis
The growth of capsular clostridium, so as to suppress the harmful substance such as phenol, ammonia, indoles caused by spoilage organisms metabolism.
The Radix Astragali has the effect of QI invigorating Gu Wei, tonifying Qi and lifting yang, and modern pharmacological research confirms that the medicine can increase reticular endothelium system
The function of system, promote the transformation of lymphocyte, increase the quantity and quality of leucocyte, promote the generation of body antibody and divide
Secrete, can effectively strengthen the resistance of human body, have the function that stronger antiviral.Astragalus polyose (APS), which has, significantly exempts from
Epidemic disease adjustment effect, the conversion ratio of lymphocyte can be improved, improve the former immune response ability of body fight, strengthen macrophage
Phagocytic activity, the immunologic function under some drugses and different stressed conditions, which is declined, has notable immunological enhancement.
Panaxan can significantly increase the phagocytic function of peritoneal macrophage, activation reticuloendothelial system (RES).Wang Benxiang
Find that panaxan can strengthen the phagocytic function of reticular endothelium layer system Deng research, promote antibody formation improve mouse IgG and
Generations of the content of guinea pig complement to mouse hemolysin also has obvious stimulation.
The content of the invention
A kind of hay-scented composition of medicine is provided on the existing Research foundation of said extracted thing, it is characterised in that:Described group
Composite medicine is made up of the extract of Chinese artichoke, the Radix Astragali, red ginseng and the banksia rose.
The composition of medicine by extracted from Chinese artichoke stachyose, extract from the Radix Astragali to Thick many candies, from red ginseng
Extraction obtains the volatile oil of Radix Aucklandiae composition extracted in Thick many candies and the worm banksia rose.
The Chinese artichoke stachyose, Radix Astragali Thick many candies, the weight ratio of red ginseng Thick many candies and volatile oil of Radix Aucklandiae dry product are 1-4:
1-2:1-2:1-2。
Described Chinese artichoke stachyose, Radix Astragali Thick many candies, the weight 2 of red ginseng Thick many candies and volatile oil of Radix Aucklandiae dry product:1:1:
1。
The preparation method of described hay-scented composition of medicine, comprises the following steps:
1) by stachyose fresh goods, 5-10min is boiled, is cooled down, is crushed, 30-60 DEG C of temperature extraction takes 40-70min, collects water
Liquid, it is concentrated and dried, obtains wood sugar extract;
2) red ginseng is softened through vapor, pelletizing, dries, crushes, sieving, add 10 times of amount water, soaked overnight, backflow carries
2-4h is taken, is filtered, filtrate centrifugation, takes supernatant, adds absolute ethyl alcohol to be adjusted to ethanol volume more than 70%, alcohol precipitation, filters, precipitation is used
Absolute ethyl alcohol, dry, obtain red ginseng polysaccharide;
3) Radix Astragali is cut into slices, adds white lime, adjust pH value to 9-10, with 10 times of water of medicinal material weight, refluxing extraction 2-4h,
Filtration, filtrate centrifugation, takes supernatant, adds absolute ethyl alcohol to be adjusted to ethanol volume more than 70%, alcohol precipitation, filters, the anhydrous second of precipitation
Alcohol, dry, obtain astragalus polyose;
4) banksia rose is cut into slices, be soaked in water 1h, and steam distillation extraction 8h, extraction obtains volatile oil of Radix Aucklandiae;
5) each group polysaccharide and volatile oil are mixed according to part by weight, obtains hay-scented composition of medicine.
Hay-scented composition of medicine described above is preparing the application in treating anti-diarrhea drug.
The method have the benefit that:Composition of medicine provided by the invention, there is good activity to treatment diarrhoea,
A variety of formulations can be developed into.
Embodiment
The activity investigation of the anti-castor oil lead to diarrhea of the hay-scented composition of medicine of embodiment 1
The preparation of hay-scented composition of medicine
By stachyose fresh goods, 5-10min to be boiled, is cooled down, is crushed, 30-60 DEG C of temperature extraction takes 40-70min, collects aqueous,
It is concentrated and dried, obtains stachyose;
Red ginseng is softened through vapor, pelletizing, dry, crush, sieving, add 10 times of amount water, soaked overnight, refluxing extraction
2-4h, filtration, filtrate centrifugation, supernatant is taken, add absolute ethyl alcohol to be adjusted to ethanol volume more than 70%, alcohol precipitation, filter, precipitation nothing
Water-ethanol, dry, obtain red ginseng polysaccharide;
The Radix Astragali is cut into slices, adds white lime, pH value is adjusted to 9-10, with 10 times of water of medicinal material weight, refluxing extraction 2-4h, filter
Cross, filtrate centrifugation, take supernatant, add absolute ethyl alcohol to be adjusted to ethanol volume more than 70%, alcohol precipitation, filter, precipitation absolute ethyl alcohol,
Dry, obtain astragalus polyose;
The banksia rose is cut into slices, be soaked in water 1h, and steam distillation extraction 8h, extraction obtains volatile oil of Radix Aucklandiae.
It is 1 according to stachyose, red ginseng polysaccharide, astragalus polyose and volatile oil of Radix Aucklandiae weight ratio:1:1:1 mixing each group polysaccharide,
Obtain I group of experiment;
It is 2 according to stachyose, red ginseng polysaccharide, astragalus polyose and volatile oil of Radix Aucklandiae weight ratio:1:1:1 mixing each group polysaccharide,
Obtain II group of experiment;
It is 1 according to stachyose, red ginseng polysaccharide, astragalus polyose and volatile oil of Radix Aucklandiae weight ratio:2:2:2 mixing each group polysaccharide,
Obtain III group of experiment;
It is 4 according to stachyose, red ginseng polysaccharide, astragalus polyose and volatile oil of Radix Aucklandiae weight ratio:1:1:1 mixing each group polysaccharide,
Obtain IV group of experiment.
Prevention effect of the hay-scented composition of medicine to castor oil lead to diarrhea
The 20g of fasting 12 hours or so Kunming mouse 90 is taken, 9 groups is divided into, every group 10, is respectively put into special
In small wire mesh cage (cage underlay blotting paper), per 1, cage, an ig red ginsengs polysaccharide, astragalus polyose, stachyose, banksia rose volatilization respectively
Oil, I group of experiment, II group of experiment, III group of experiment, experiment IV group and Diarrhoea's Killer and distilled water (control group).After 30 minutes, equal ig
Lead to diarrhea agent castor oil 0.2ml/10g.1 hour start recording loose stools number after to lead to diarrhea agent, per hour once, totally 5 hours.With every
The mouse loose stools number of 5 hours is added, and calculates average and standard deviation, and suppresses %, and examines with t that to survey it aobvious with control group
Write sex differernce.
The hay-scented composition of medicine of table 1 to the prevention effect of castor oil lead to diarrhea (N=10)
Note:F/T~tabular form animal number, F represent loose stools number of animals.
As a result (being shown in Table 1) is found, after the hay-scented composition of medicine of ig different formulations ratios, loose stools number also has different degrees of subtract
Few, with the increase of dosage, its reduction degree is more obvious, shows obvious dose-effect relationship.It can be seen that hay-scented composition of medicine have it is bright
Diarrhoea caused by aobvious prevention castor oil acts on.After administration, the diarrhoea of part mouse can be completely inhibited.Diarrhoea's Killer also has very bright
Aobvious inhibitory action.Stachyose, astragalus polyose, red ginseng polysaccharide, volatile oil of Radix Aucklandiae are respectively provided with caused by certain prevention castor oil
Diarrhoea acts on, but its effect is significantly lower than composition of medicine.
Hay-scented composition of medicine causes the therapeutic action of diarrhea of mouse to castor oil:
19~21g Kunming mouse 60 is taken, is divided into 6 groups, every group 10, male and female half and half.Equal ig castor oil
0.1ml/10g, after 30 minutes, ig red ginsengs polysaccharide, astragalus polyose, stachyose, volatile oil of Radix Aucklandiae, I group of experiment, experiment II respectively
Group, III group of experiment, experiment IV group and Diarrhoea's Killer and distilled water (control group), record to before lead to diarrhea agent and to after lead to diarrhea agent 1,2,
The loose stools sum of 3,4 and 5 hours, calculates the mean+SD of every group of animal loose stools, and suppresses percentage, and with compareing
Group is compared, through " t " examine survey its significant difference.
The hay-scented composition of medicine of table 2 to castor oil lead to diarrhea therapeutic action (n=10,)
Note:F/T~tabular form animal number, F represent loose stools number of animals.
Table 2 is the therapeutic action that ig hay-scenteds composition of medicine causes diarrhea of mouse to castor oil.From table 2, the hay-scented of formula
Composition of medicine can suppress to suffer from diarrhoea caused by castor oil, and its inhibiting rate is 20.3~69.5%, it is seen that ig hay-scented composition of medicine
The catharsis effect of castor oil can substantially be treated.Stachyose, astragalus polyose, red ginseng polysaccharide and volatile oil of Radix Aucklandiae are respectively provided with certain control
Diarrhoea effect caused by castor oil is treated, but its effect is significantly lower than composition of medicine.
Influence of the hay-scented composition of medicine to sennae lead to diarrhea
The Kunming kind hero mouse 50 of fasting 4 hours is taken, respectively 5 groups, every group 10, difference ig tests II group, experiment III
Group, IV group of experiment, Diarrhoea's Killer 0.5g/kg, control group gives distilled water, and its volume is 0.2ml/10g body weight, after half an hour,
Equal ig 0.2ml/ mouse of folium sennae.Observe and record to 1 after lead to diarrhea agent, the loose stools number of 2,3,4, and 5 hours, calculate every group and move
The mean+SD of thing loose stools, and suppress percentage, and compared with control group, through " t " examines and survey its significant difference.
As a result it is as shown in table 3.
The hay-scented composition of medicine of table 3 to folium sennae cause diarrhea of mouse prevention effect (N=10)
Note:F/T~tabular form animal number, F represent loose stools number of animals.
From table 3, to the mouse of the hay-scented composition of medicine of various dose, diarrhoea number is significantly reduced, and shows grass
Fragrant composition of medicine has obvious inhibitory action to diarrhoea caused by sennae, and its inhibiting rate is 46.7~91.6%.Wherein test
II group of effect is the most notable;Diarrhoea's Killer also has obvious effect.
The another Kunming kind hero mouse 50 for taking fasting 4 hours, respectively 5 groups, every group 10, equal ig folium sennaes 0.2ml/ is only small
Mouse, after half an hour, ig difference ig tests II group, tests III group, IV group of experiment respectively, and Diarrhoea's Killer 0.5g/kg, control group gives steaming
Distilled water, its volume are 0.2ml/10g body weight.Observe and record to 1 after lead to diarrhea agent, the loose stools number of 2,3,4, and 5 hours, calculate
The mean+SD of every group of animal loose stools, and suppress percentage, and compared with control group, through " t " examines that to survey its notable
Sex differernce.As a result it is as shown in table 4.
The hay-scented composition of medicine of table 4 to folium sennae cause diarrhea of mouse therapeutic action (N=10)
Note:F/T~tabular form animal number, F represent loose stools number of animals.
From table 4, after ig is to the hay-scented composition of medicine of medicine composition, diarrhoea number is significantly reduced, and shows hay-scented group
Composite medicine has obvious inhibitory action to diarrhoea caused by sennae, and its inhibiting rate is 42.9~76.2%.Wherein test II group
Effect it is the most notable;Wherein possible, this effect is due to that hay-scented composition of medicine reduces stimulation of the sennae to small intestine, from
And subtract the contraction of intestines.
The therapeutic action of the anti-rheum officinale lead to diarrhea of hay-scented composition of medicine
Take 19-22g mouse 50, be divided into 5 groups, every group 10 (male and female half and half), ig red ginsengs polysaccharide, astragalus polyose,
Stachyose, volatile oil of Radix Aucklandiae, I group of experiment, II group of experiment, III group of experiment, IV group of experiment and Diarrhoea's Killer and distilled water (control
Group), given with 20ml volumes/kg.45 minutes after administration, ig rheum officinales 1.0g/kg, mouse loose stools is counted by preceding after 1 hour
Number, per hour once, altogether 5 hours of number, was represented, and compared with control group, survey it with the wet just standard deviation of mean of 6 hours
The significance of difference;Concrete outcome is as shown in table 5.
Table 5, hay-scented composition of medicine to rheum officinale lead to diarrhea therapeutic action (n=10,)
Note:F/T~tabular form animal number, F represent loose stools number of animals.
Table 5 is the effect that hay-scented composition of medicine resists diarrhoea caused by rheum officinale.Formula hay-scented composition of medicine is given, is substantially subtracted
When young mouse is wet just counts, and inhibiting rate has a certain amount-effect relation between 38.2~62.8, compared with control group, has bright
Aobvious difference.Red ginseng polysaccharide, astragalus polyose, volatile oil of Radix Aucklandiae and stachyose folk prescription are respectively provided with certain anti-diarrhea effect, but effect
Significantly lower than composition of medicine.
The prevention effect of the anti-rheum officinale lead to diarrhea of hay-scented composition of medicine
Take 19-22g mouse 50, be divided into 5 groups, every group 10 (male and female half and half), ig red ginsengs polysaccharide, astragalus polyose,
Stachyose, volatile oil of Radix Aucklandiae, I group of experiment, II group of experiment, III group of experiment, IV group of experiment and Diarrhoea's Killer and distilled water (control
Group), given with 20ml volumes/kg.45 minutes after administration, ig rheum officinales 1.0g/kg, mouse loose stools is counted by preceding after 1 hour
Number, per hour once, altogether 5 hours of number, was represented, and compared with control group, survey it with the wet just standard deviation of mean of 6 hours
The significance of difference.Concrete outcome is as shown in table 5.
The hay-scented composition of medicine of table 5 to the prevention effect of rheum officinale lead to diarrhea (n=10,)
Note:F/T~tabular form animal number, F represent loose stools number of animals.
Table 5 is the effect that hay-scented composition of medicine resists diarrhoea caused by rheum officinale.It is small after hay-scented composition of medicine from table 2
The wet just number of mouse significantly reduces, and also and has obvious amount-result relation, its effect is similar to its therapeutic action, and (inhibiting rate is 34.4
~80.6%) red ginseng polysaccharide, volatile oil of Radix Aucklandiae, astragalus polyose and stachyose folk prescription are respectively provided with certain anti-diarrhea effect, but effect
Significantly lower than composition of medicine.
Influence of the hay-scented composition of medicine of embodiment 2 to Small intestine advance activity
Influence to small intestine movement of mice advancing movement:Take fasting 12 hours, body weight 19-20g Kunming mouse, by body
5 groups are randomly divided into again, every group 11, distinguish ig red ginsengs polysaccharide, astragalus polyose, stachyose, volatile oil of Radix Aucklandiae, I group of experiment, experiment
II group, III group of experiment, IV group of experiment, atropine 6mg/kg and water (positive drug group), control group gives physiological saline, 30 minutes
Afterwards, equal ig5% carbon powder suspension (10% Arabic gum system) 10ml/kg.The cervical vertebra that breaks after 20 minutes is put to death, and is cut open the belly, is taken out small intestine,
It is placed in and is placed with NS glass plate (is allowed to be in physiological status) in advance, measures the total length (stomach pylorus to ileocecus) of small intestine, and carbon
The distance (distance of pylorus to charcoal end forward position) that end promotes, calculate the percentage that charcoal end promotes in small enteral.Examined with " t " and survey it
With the difference of model group.As a result it is as shown in Table 15.
Calculation formula is:Small intestine push percentage=(distance/small intestine total length at charcoal end) × 100%
Influence of the hay-scented composition of medicine of table 6 to small intestine movement of mice advancing movement
From table 6, after normal mouse ig hay-scented composition of medicine, III group of I group of experiment, III group of experiment, experiment small intestine push away
Enter motion have it is different degrees of slow down, it is this slow down effect not as atropine effect it is obvious.Test II group of effect and atropic
Product approach.In addition, red ginseng polysaccharide, volatile oil of Radix Aucklandiae, astragalus polyose and stachyose only slightly suppress normal mouse in 10g/kg
Small intestine advance activity simultaneously has preferable repeatability.
Influence to isolated guinea pig stomach bar;Take the cavy of hungry 48 hours tap the head it is lethal after, cut open the belly and take stomach, be immediately placed in gram
In family name's liquid, stomach bottom is wiped out, gastral cavity is cut off along lesser curvature, it is parallel to cut off 5-6 roads, about 2cm stomach bars are taken, one end is connected in bath tub bottom part
Portion, another end attachment force-displacement transducer, 95%O is filled with bath2And 5%CO2Mixed gas.
Stablize and treat small intestine within 30 minutes and record baseline, add activator BaCL20.1ml (stoste 5mg/ml) or ACH0.2ml
(stoste 4mg/ml), make final concentration respectively up to 20 μ g/ml, and 3.2 μ g/ml.Observe and record the shrinkage amplitude of ileum, 3-5 points
Zhong Houyong nutrient solutions rinse, and after repeated contraction amplitude is consistent, add II group of the experiment of various concentrations (embodiment 1 is prepared)
Formula, the BaCL2 or Ach of above-mentioned concentration are added after 3-5 minutes, observes its shrinkage amplitude.Hay-scented composition of medicine is calculated to suppress
The percentage of ileum contraction.It is specific as shown in table 7.
The influence that the hay-scented composition of medicine of table 7 shrinks to stomach bar flesh
Influence to isolated rat intestinal contraction
After taking the disconnected cervical vertebra of hungry 12 hours rat lethal, cut open the belly take small intestine rapidly, be immediately placed in krebs solution, wipe out week
Tissue is enclosed, takes a length of one section of some sections of about 1.5~2.0cm, is immediately placed in after cleaning standby in fresh krebs solution.Every time with one
Section, it is connected in bath bottom, another end attachment force-displacement transducer after the ligation of one end.95%O is filled with bath2And 5%CO2Mixing
Gas, bath temperature are maintained at 37 DEG C.Bath volume about 25ml.
After stablizing 30 minutes and recording baseline, activator BaCL is added2Half portion 0.1ml (stoste 5mg/ml) or Ach0.2ml
(stoste 4mg/ml), make final concentration respectively up to 20 μ g/ml, and 3.2 μ g/ml.Observe and record the shrinkage amplitude of ileum, 3-5 points
Zhong Houyong nutrient solutions rinse, and after repeated contraction amplitude is consistent, add the hay-scented composition of medicine of various concentrations, add again after 3-5 minutes
Enter the BaCL2 or Ach of above-mentioned concentration, observe its shrinkage amplitude.Calculate the percentage that hay-scented composition of medicine suppresses ileum contraction.Meter
It is as follows to calculate work(formula:
Give hay-scented composition of medicine after-contraction amplitude to suppress the percentage shrinkage amplitude of activator=(give-and give hay-scented combination medicine
Shrinkage amplitude after thing) shrinkage amplitudes of the ÷ to activator.The drug concentration of suppression 50% is calculated, examines its difference of survey to show with t
Work property.4~5 samples of each dosage group, with 3 different concentration.
Experimental result is as shown in table 18, and the hay-scented composition of medicine of concentration used can resist Ach and BaCL2Caused small intestine
Shrink.1.25mg/ml hay-scented composition of medicine significantly acts on, increasing concentrations to 5.0mg/ml, has acted on clearly,
Inhibiting rate is respectively in 72.8% and 54.3%.
The hay-scented composition of medicine of table 8 is to Ach and BaCl2Cause the influence of isolated rat intestinal contraction
Note:It is crude drug amount in parantheses;It is 6 that Ach, which causes intestinal contraction sample,;BaCL2It is 5 to cause intestinal contraction sample
If the final concentration of hay-scented composition of medicine is respectively 6.25,12.5 and 25.0mg/ml based on crude drug.
(II group of experiment) to can substantially resist BaCL2 and Ach intestinal contractions it can be seen that hay-scented composition of medicine, with experiments in vivo
As a result it is consistent.Possible this is that abdomen stops suppressing one of principle of diarrhoea.
It by different mode alleviating diarrhoeas, can such as reduce the secretion of the wriggling and intestinal juice of intestines and absorb and can reach only
The purpose rushed down.Our Germicidal efficacy is to hay-scented composition of medicine (experiment II group) to the advancing movement of the small intestine of normal mouse and new
The mouse that this bright caused intestinal motility is hyperfunction has obvious inhibitory action.Known castor oil is in upper part of small intestine by fatty water
Solution enzyme resolves into castor oil acid excitatory, stimulates small intestine to cause enterocinesia to increase and suffer from diarrhoea.Hay-scented composition of medicine can be obvious
Suppress normal and hyperfunction small intestine wriggling, thus it may be mainly to suppress the wriggling of small intestine and play work that it, which suppresses the effect of diarrhoea,
With.
Influence of the hay-scented composition of medicine of embodiment 10 to stomachache
The influence of writhing response caused by Dichlorodiphenyl Acetate:Take 20g or so mouse 44, respectively 4 groups.Ig red ginsengs are more respectively
Sugar, astragalus polyose, stachyose, volatile oil of Radix Aucklandiae, I group of experiment, II group of experiment, III group of experiment, IV group of experiment, indocin 0.06g/
Kg, control group give the Tween 80 of same volume 0.6% or distilled water, 45 minutes equal ip0.9% acetic acid 0.1ml/10g bodies after administration
Weight.The number of its writhing response is recorded after 5 minutes, is observed 15 minutes altogether.With " t " examine survey its significant difference with control group
Property, its result is as shown in table 9.
The hay-scented composition of medicine Dichlorodiphenyl Acetate of table 9 causes the influence of mouse writhing reaction
The influence of pain is caused to thermostimulation
Take (the experiment the previous day pre-selection, to incubation period of thermostimulation 15 of female KM kind mouse 50 of fasting 14 hours
Mouse in second), 5 groups are divided into by body weight, ig tests II group 2.5,5.0,10.0g/kg respectively, and control group gives distilled water,
Its volume is 0.2ml/10g body weight, 30 and 60 minutes after administration, is put into and is placed on the metallic plate in water-bath, its temperature is led to
Water bath with thermostatic control regulation is crossed at 55 ± 0.5 DEG C.Hot plate to the reaction time (incubation period) for occurring adding metapedes is put into using mouse and is used as pain
The index of threshold.The front and rear variable value of administration is calculated, is examined with " t " and surveys its poor conspicuousness, or time corresponding to control group electricity
Latent variable value compares, and surveys its significant difference.
As a result it is as shown in table 10.
The hay-scented composition of medicine of table 10 to thermostimulation induced pain influence (N=10)
Note:Compared before and after administration, * P<0.05,***P<0.001;▲P<0.001.
From 10, to different time after hay-scented composition of medicine 5.0 and 10.0g/kg, bitterly preclinical extension unobvious,
But the incubation period of positive drug indocin is then obviously prolonged.Show hay-scented composition of medicine to pain caused by thermostimulation without obvious confrontation
Effect.
Above-mentioned experiment is visible, and hay-scented composition of medicine can substantially suppress writhing response caused by acetic acid, and its effect is probably logical
Cross caused by the intestinal smooth muscle spasm caused by acetic acid that relaxes;And central pain caused by thermostimulation is also had certain effect, but
Be not as obvious as spasmolysis.
Under sterile working, the culture of nutrition meat extract soup is taken to be based in test tube with transfer pipet(te), often pipe 5ml, then suct and state sample
Product solution 5ml is put into culture medium, is 1 after fully shaking up:2, doubling dilution is into 1 successively:4、1:8、1:6、1:32、1:64、1:
128、1:256、1:512, its drug concentration is followed successively by 500,250,125,62.5,31.2,15.6 and 7.8mg/ml, separately takes and is free of
The culture medium of medicine is as control tube.
The investigation of the hay-scented composition of medicine antibacterial action of embodiment 10
Each experimental strain is inoculated in nutrient broth medium, cultivates 16~18 hours at 37 DEG C, is trained with nutrient broth
Support base or sterile saline is diluted to 10-3Concentration.Take concentration dilution bacterium solution 0.1ml to add the medicine being ready for (to use
Acalypha copperleaf in embodiment 1:Purslane (7:3) group, following each group are identical) in broth bouillon, mix small after 37 DEG C of cultures 24
When, observe bacterial growth situation.Such as drug liquid tube clarification, no bacterial growth is indicated, is such as muddiness, represents that bacterium has grown.With complete
Completely without MIC of the minimum drug concentration as the medicine to the bacterial strain of bacterial growth.
From table 11, hay-scented composition of medicine (II group of experiment) is to Shigella shigae, shigella flexneri, Boydii
Shigella dysenteriae, S. sonnei and Escherichia coli have a certain degree of inhibitory action, their minimal inhibitory concentration (MIC)
Respectively 31.2,31.2,31.2,125.0 and 125.0mg/ml, the effect to former three are stronger.
Measure of the hay-scented composition of medicine of table 11 to different strains minimum inhibitory concentration
Hay-scented composition of medicine (experiment II group) is to surveyed Shigella shigae, shigella flexneri, Boydii shigella dysenteriae,
S. sonnei and Escherichia coli have different degrees of inhibitory action.
Claims (4)
- A kind of 1. hay-scented composition of medicine, it is characterised in that:The composition of medicine by Chinese artichoke, the Radix Astragali, red ginseng and the banksia rose extraction Thing forms;The composition of medicine by extracted from Chinese artichoke stachyose, extract from the Radix Astragali to Thick many candies, extract from red ginseng The volatile oil of Radix Aucklandiae composition for obtaining Thick many candies and being extracted from the banksia rose;The Chinese artichoke stachyose, Radix Astragali Thick many candies, the weight ratio of red ginseng Thick many candies and volatile oil of Radix Aucklandiae are 1-4:1-2:1-2: 1-2。
- 2. hay-scented composition of medicine according to claim 1, it is characterised in that:Described Chinese artichoke stachyose, the Radix Astragali are slightly more The weight 2 of sugar, red ginseng Thick many candies and volatile oil of Radix Aucklandiae:1:1:1.
- 3. the preparation method of the hay-scented composition of medicine described in claim 1,2 any one, it is characterised in that:Comprise the following steps:1) by Chinese artichoke fresh goods, 5-10min is boiled, is cooled down, is crushed, 30-60 DEG C of temperature extraction takes 40-70min, collects aqueous, dense Contracting drying, obtain wood sugar extract;2) red ginseng is softened through vapor, pelletizing, dries, crushes, sieving, add 10 times of amount water, soaked overnight, refluxing extraction 2- 4h, filtration, filtrate centrifugation, supernatant is taken, add absolute ethyl alcohol to be adjusted to ethanol volume more than 70%, alcohol precipitation, filter, precipitate with anhydrous Ethanol washs, and dries, obtains red ginseng polysaccharide;3) Radix Astragali is cut into slices, adds white lime, pH value is adjusted to 9-10, with 10 times of water of medicinal material weight, refluxing extraction 2-4h, filter Cross, filtrate centrifugation, take supernatant, add absolute ethyl alcohol to be adjusted to ethanol volume more than 70%, alcohol precipitation, filter, precipitation absolute ethyl alcohol Washing, dry, obtain astragalus polyose;4) banksia rose is cut into slices, be soaked in water 1h, and steam distillation extraction 8h, extraction obtains volatile oil of Radix Aucklandiae;5) each group polysaccharide and volatile oil are mixed according to part by weight, obtains hay-scented composition of medicine.
- 4. the hay-scented composition of medicine described in claim 1,2 any one is preparing the application in treating anti-diarrhea drug.
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CN102861124A (en) * | 2012-10-29 | 2013-01-09 | 重庆师范大学 | Costus root combination medicine and application thereof |
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