CN104287865B - A kind of animal spinal cord living imaging Orientation observation device - Google Patents
A kind of animal spinal cord living imaging Orientation observation device Download PDFInfo
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- CN104287865B CN104287865B CN201410571850.7A CN201410571850A CN104287865B CN 104287865 B CN104287865 B CN 104287865B CN 201410571850 A CN201410571850 A CN 201410571850A CN 104287865 B CN104287865 B CN 104287865B
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- adjusting means
- wishbone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/40—Detecting, measuring or recording for evaluating the nervous system
- A61B5/4058—Detecting, measuring or recording for evaluating the nervous system for evaluating the central nervous system
- A61B5/407—Evaluating the spinal cord
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/48—Other medical applications
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Abstract
A kind of animal spinal cord living imaging Orientation observation device, it by base plate (1), left side move adjusting means (2), adjusting means (3) is moved on right side and implanted observation window (4) forms；The mobile adjusting means in left side (2), right side are moved adjusting means (3) and are fixed on base plate (1) by screw, implanted observation window (4) by the rectangular opening on its V-type frame and left side move adjusting means (2), right side is moved the rectangular preiection of the horizontal location slide block in adjusting means (3) and is plugged together positioning.The present invention can either be conducive to the installation of implanted observation window to perform the operation, and the stability making backbone is more preferable, can be conducive to again the dynamic observation of postoperative two-photon spinal cord imaging.Left and right side moves the horizontal location slide block in adjusting means can not only provide horizontal direction stable interior stationary state to the operation of implanting device, gives stable extenal fixation and support when more can observe after surgery.
Description
Technical field:
The present invention relates to a kind of animal spinal cord living imaging Orientation observation device, it is used for the reality in Neurobiology field
Test in research, the installation operation of implanted observation window can be carried out to animal, and live body can be carried out for a long time and dynamically observe spinal cord
Nerve fiber and the change of blood vessel thereof, physiology and the pathological change to research nervous centralis particularly spinal cord injury, and spinal cord
Regeneration neural after damage has very big value with reparation.
Background technology:
In the experimental study in Neurobiology field, traditional animal spinal cord living imaging means are excision animals one
The vertebral plate of sections thus exposing spinal cord, after using whole body suspension method or local Clamping stablize backbone, use fluorescence microscope or
Person's Two Photon Fluorescence carries out the dynamic imaging of live body to the animal spinal cord exposing.Imaging terminates rear layer-by-layer suture muscle and skin
Skin, cuts skin and muscle when observing next time again, exposes original spinal segment with this repeatedly.But this process can not be held
Continuing for a long time, anesthesia and repeat surgery not only can bring the harm of whole body function to animal for a long time, more can improve animal sense
The risk of dye, increases inflammatory reaction, causes tissue damage, causes animal more painful, is unfavorable for further observing and real
Test research.And the operation currently without unified standard is fixing and clinical follow external fixing rack, causes operation technique complicated, postoperative
The fixing difficulty of imaging.
Content of the invention:
1st, purpose: it is an object of the invention to provide a kind of animal spinal cord living imaging Orientation observation device.It can either have
Being beneficial to the installation operation of implanted observation window, the stability making backbone is more preferable, can be conducive to again postoperative two-photon spinal cord imaging
Dynamic observation.The horizontal location slide block of living imaging external fixing rack provides horizontal direction stable can not only to the operation of implanting device
Interior stationary state, give stable extenal fixation when more can observe after surgery and support.
2nd, technical scheme: the technical scheme is that a kind of animal spinal cord living imaging Orientation observation device, it be by
Base plate the 1st, left side is moved adjusting means the 2nd, right side and is moved adjusting means 3 and implanted observation window 4 forms；Position between them is even
The relation of connecing is: left side is moved adjusting means the 2nd, right side and moved adjusting means 3 and be fixed on base plate 1 by screw, and implanted is observed
Window 4 moves, by the rectangular opening on its V-type frame and left side, the horizontal location slide block that adjusting means the 2nd, right side is moved in adjusting means 3
Rectangular preiection plug together positioning.
Described base plate 1 is one piece of rectangle plate, around both sides be each provided with two install screwed holes move for left side
The fixing of adjusting means 3 is moved on adjusting means the 2nd, right side.
It is by dovetail groove base 2-1, horizontal location slide block 2-2 and positioning screw 2-3 group that adjusting means 2 is moved in described left side
Become；Position annexation between them is: this dovetail groove base 2-1 is dovetail-shape in the horizontal direction, so that
It is similarly the horizontal location slide block 2-2 bilateral reciprocation within it of swallow-tail form；When sliding into target location, screw positioning spiral shell
Nail 2-3, positioning screw withstands dovetail groove base 2-1, reaches to prevent dovetail groove base 2-1 relative sliding with horizontal location slide block 2-2
Dynamic purpose.This rectangular shape of dovetail groove base 2-1 profile, horizontal direction is provided with swallow tail shaped slot, and vertical direction is rectangular block.Should
Horizontal location slide block 2-2 is that rectangular slab materials and parts process, and the screw that it is arranged is used for the connection with positioning screw 2-3 and determines
Position, end is processed into rectangular preiection and is easy to plug together positioning with the rectangular opening on wishbone；Afterbody is processed into dovetail form to be easy to swallow
Slide in stern notch base 2-1.This positioning screw 2-3 is commercial connector.
Adjusting means 3 and left side are moved in described right side, and to move adjusting means 2 structure identical, and specular is installed；
Described implanted observation window 4 is by left side wishbone 4-1, right side wishbone 4-2, strip pad 4-3, rectangle lid
Slide 4-4 and installation screw 4-5 composition；Position annexation between them is: left side wishbone 4-1 and right side wishbone 4-2
On be respectively provided with two screwed holes, after position is fixing, is vertically arranged in strip pad 4-3 on left and right sides wishbone screwed hole, passes through
Screw 4-5 is installed strip pad 4-3 is fixed with left and right sides wishbone.In the middle of two strip pad 4-3, length can be inserted
Square cover slide 4-4.Distance between the wishbone 4-1 and right side wishbone 4-2 of left side can be by the regulation of strip pad 4-3
Hole is adjusted.
The operation principle of the present invention and flow process brief introduction are as follows:
The operation principle of the present invention is: by living imaging external fixing rack and implanted observation window, it is achieved install animal ridge
The purpose of marrow implanted observation window, and then postoperative use Two Photon Fluorescence carries out living imaging to spinal cord, thus completes live body
The operation that observation spinal cord neural cell dynamically changes.
The workflow of the present invention is: for further illustrating technical scheme, the existing operation carrying out the present invention
Operating process is briefly described as follows:
1st, operation installed by implanted observation window: by Animal Anesthesia, uses iodophor disinfection, be placed on base plate after the shaving of back,
Cut skin, cleaning chest 13-waist 2 vertebral plate Surrounding muscles and soft tissue at chest 13-waist 2 projection of edge back part of animal central authorities, appear chest
13-waist 2 vertebral plate.
2nd, both sides horizontal location slide block is stretched into wishbone slotted eye, i.e. both sides wishbone load level positioning sliding block, level
Slip horizontal location slide block, makes both sides wishbone clamping chest 13-waist 2 centrum.
3rd, use micro-scissors by waist 1 vertebrae plate resection, fully expose waist 1 spinal segment.
4th, strip pad is positioned over above both sides wishbone and spinal cord, tightens four screws.
5th, one layer of biological silicone rubber is covered on spinal cord surface, by rectangle cover glass covering resin.Use speed
Implanted observation window is fixed and closed to dry strength glue and dental base acrylic resin powder, by both sides horizontal location slide block after seccotine and dental base acrylic resin powder solidification
Removal both sides V-type frame slotted eye, skin suture operation completes.
6th, Two Photon Fluorescence is observed fixing: by Animal Anesthesia, is positioned over living imaging external fixing rack central authorities.By both sides water
Flat positioning sliding block level is slided, and stretches into wishbone both sides slotted eye, fixing implanted observation window and animal backbone.
7th, Two Photon Fluorescence is used to carry out live body neuroimaging to animal spinal cord.
8th, observation completes, by both sides horizontal location slide block removal both sides V-type frame slotted eye.
The invention have the advantages that
1st, implanted observation window is conducive to using Two Photon Fluorescence to carry out long-term live body to animal spinal cord and dynamically observe, and keeps away
Exempt from the problem that repeat surgery brings.
2nd, external fixing rack not only contributes to the carrying out that operation installed by implanted observation window, is more beneficial for postoperative animal spinal cord and sees
That examines is fixing.
3rd, this system is applied widely, can be used for the large-scale experiment animals such as rat, rabbit, dog, monkey after change size.
Brief description
Fig. 1 is the structure chart of this animal spinal cord living imaging Orientation observation device.
Fig. 2 a is the structure chart of implanted observation window.
Fig. 2 b is wishbone schematic diagram.
Fig. 2 c is strip pad schematic diagram.
Fig. 2 d is rectangle cover glass schematic diagram.
Fig. 3 a is the structure chart of mobile adjusting means.
Fig. 3 b is horizontal location slide block schematic diagram.
Fig. 3 c is dovetail groove base schematic diagram.
Fig. 4 is base plate schematic diagram.
In figure, sequence number is described as follows:
1st, base plate；2nd, adjusting means is moved in left side；3rd, adjusting means is moved on right side；4th, implanted observation window；
2-1, dovetail groove base；2-2, horizontal location slide block；2-3, positioning screw；
4-1, left side wishbone；4-2, right side wishbone；4-3, strip pad；4-4, rectangle cover glass；4-5, installation
Screw.
Detailed description of the invention
See Fig. 1-Fig. 4, the technical scheme is that a kind of animal spinal cord living imaging Orientation observation device, it be by
Base plate the 1st, left side is moved adjusting means the 2nd, right side and is moved adjusting means the 3rd, implanted observation window 4 and form；Position between them is even
The relation of connecing is: left side is moved adjusting means the 2nd, right side and moved adjusting means 3 and be fixed on base plate 1 by screw, and implanted is observed
Window 4 moves adjusting means the 2nd, right side by rectangular opening on it and left side and moves the rectangular preiection of adjusting means 3 and plug together positioning.
Described installation 1 is one piece of rectangle plate, and both sides respectively arrange two installation screwed holes and move tune for left side around
The fixing of adjusting means 3 is moved on regulating device the 2nd, right side.
It is by dovetail groove base 2-1, horizontal location slide block 2-2 and positioning screw 2-3 group that adjusting means 2 is moved in described left side
Become；Position annexation between them is: this dovetail groove base 2-1 is dovetail-shape in the horizontal direction, so that
It is similarly the horizontal location slide block 2-2 bilateral reciprocation within it of swallow-tail form；When sliding into target location, screw positioning spiral shell
Nail 2-3, positioning screw withstands dovetail groove base 2-1, reaches to prevent dovetail groove base 2-1 relative sliding with horizontal location slide block 2-2
Dynamic purpose.This rectangular shape of dovetail groove base 2-1 profile, horizontal direction is provided with swallow tail shaped slot, and vertical direction is rectangular block.Should
Horizontal location slide block 2-2 is that rectangular slab materials and parts process, and the screw that it is arranged is used for the connection with positioning screw 2-3 and determines
Position, end is processed into rectangular preiection and is easy to plug together positioning with the rectangular opening on wishbone；Afterbody is processed into dovetail form to be easy to swallow
Slide in stern notch base 2-1.This positioning screw 2-3 is commercial connector.
Adjusting means 3 and left side are moved in described right side, and to move adjusting means 2 structure identical, and mirror image is installed；
Described implanted experiment porch 4 is by left side wishbone 4-1, right side wishbone 4-2, strip pad 4-3, rectangle
Cover glass 4-4 and installation screw 4-5 composition；Position annexation between them is: left side wishbone 4-1 and right side wishbone 4-
It is respectively arranged with two screwed holes on 2, after position is fixing, is vertically arranged in strip pad 4-3 on left and right sides wishbone screwed hole, passes through
Screw 4-5 is installed strip pad 4-3 is fixed with left and right sides wishbone.In the middle of two strip pad 4-3, length can be inserted
Square cover slide 4-4.Distance between the wishbone 4-1 and right side wishbone 4-2 of left side can be by the regulation of strip pad 4-3
Hole is adjusted.
Being schematic above by reference to the accompanying drawing description of this invention, not having restricted, those skilled in the art should
It is understood that in reality is implemented, in the present invention, shape and the layout type of each component are equal it may happen that some changes；And at this
Under the enlightenment of invention, other staff also may be made that the design similar to the present invention or modify the present invention and certain structure
The equivalent of part.Special needs to be pointed out is, without departing from the design aim of the present invention, all obvious change with
And there is the similar Design of equivalent, it is all contained within protection scope of the present invention.
Claims (1)
1. an animal spinal cord living imaging Orientation observation device, it is to be moved adjusting means (2), right side by base plate (1), left side
Mobile adjusting means (3) and implanted observation window (4) composition；Adjusting means (3) is moved on the mobile adjusting means (2) in left side, right side
Being fixed on base plate (1) by screw, implanted observation window (4) moves regulation dress by the rectangular opening on its V-type frame and left side
Put (2), the rectangular preiection of horizontal location slide block on adjusting means is moved on right side (3) plugs together positioning；
It is characterized in that:
It is by dovetail groove base (2-1), horizontal location slide block (2-2) and positioning screw (2-that adjusting means (2) is moved in described left side
3) form；This dovetail groove base (2-1) is dovetail-shape in the horizontal direction so that the horizontal location being similarly swallow-tail form is sliding
Block (2-2) bilateral reciprocation within it；When sliding into target location, screwing positioning screw (2-3), positioning screw withstands swallow
Stern notch base (2-1), reaches to prevent the purpose of dovetail groove base (2-1) slip relative with horizontal location slide block (2-2)；This dovetail
The rectangular shape of bottom land seat (2-1) profile, horizontal direction is provided with swallow tail shaped slot, and vertical direction is rectangular block；This horizontal location slide block
(2-2) being that rectangular slab materials and parts process, the screw that it is arranged is for the connection and positioning, end with positioning screw (2-3)
Being processed into rectangular preiection to be easy to plug together positioning with the rectangular opening on wishbone, afterbody is processed into dovetail form to be easy at the bottom of dovetail groove
Slide in seat (2-1)；
Described base plate (1) is one piece of rectangle plate, and both sides are each provided with two installation screwed holes and move tune for left side around
Regulating device (2), right side move the fixing of adjusting means (3)；
Adjusting means (3) and left side are moved in described right side, and to move adjusting means (2) structure identical, and specular is installed；
Described implanted observation window (4) is by left side wishbone (4-1), right side wishbone (4-2), strip pad (4-3), rectangular
Shape cover glass (4-4) and installation screw (4-5) composition；It is respectively provided with two spiral shells on left side wishbone (4-1) and right side wishbone (4-2)
Strip pad (4-3), after position is fixing, is vertically arranged on left and right sides wishbone screwed hole by pit, by installing screw (4-
5) strip pad (4-3) and left and right sides wishbone are fixed；In the middle of two strip pads (4-3), insert rectangle cover glass
(4-4), the distance between left side wishbone (4-1) and right side wishbone (4-2) is then entered by the adjustment hole of strip pad (4-3)
Row regulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410571850.7A CN104287865B (en) | 2014-10-23 | 2014-10-23 | A kind of animal spinal cord living imaging Orientation observation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410571850.7A CN104287865B (en) | 2014-10-23 | 2014-10-23 | A kind of animal spinal cord living imaging Orientation observation device |
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| Publication Number | Publication Date |
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| CN104287865A CN104287865A (en) | 2015-01-21 |
| CN104287865B true CN104287865B (en) | 2016-11-09 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104771145B (en) * | 2015-04-14 | 2017-04-12 | 中国人民解放军总医院 | Animal dorsal root ganglion-spinal cord fixed imaging and compression injury combining device |
| CN108784881B (en) * | 2018-03-28 | 2023-05-16 | 中国人民解放军总医院 | Mouse kidney living body microscopic imaging and tracing matching system |
| CN111419181B (en) * | 2020-03-31 | 2023-07-25 | 浙江大学 | Device for long-time living body imaging of small animals and application method thereof |
| CN113413231A (en) * | 2021-06-21 | 2021-09-21 | 深圳市人民医院 | Fixed observation device for animal spinal nerve living body imaging and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8078256B2 (en) * | 2002-10-10 | 2011-12-13 | Visualsonics Inc. | Integrated multi-rail imaging system |
| CN202006288U (en) * | 2011-02-19 | 2011-10-12 | 洪青强 | Fast clamping chuck |
| CN102871768A (en) * | 2012-09-25 | 2013-01-16 | 南京航空航天大学 | Big gecko's vertebral column three-dimensional positioning device and method |
| CN103284812A (en) * | 2013-06-29 | 2013-09-11 | 吴爱悯 | Outer fixing support for spinal cord injury animal experiments |
| CN103712613B (en) * | 2013-12-13 | 2016-04-13 | 上海交通大学 | The disc type micromechanics solid fluctuation gyro packaging system that dovetail groove slides and method |
| CN204092265U (en) * | 2014-10-23 | 2015-01-14 | 中国人民解放军总医院 | A kind of animal spinal cord living imaging Orientation observation device |
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