CN104262049A - Preparation method of biological organic fertilizer - Google Patents

Preparation method of biological organic fertilizer Download PDF

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Publication number
CN104262049A
CN104262049A CN201410489085.4A CN201410489085A CN104262049A CN 104262049 A CN104262049 A CN 104262049A CN 201410489085 A CN201410489085 A CN 201410489085A CN 104262049 A CN104262049 A CN 104262049A
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China
Prior art keywords
biological organic
preparation
chemical fertilizer
organic chemical
viable count
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CN201410489085.4A
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Chinese (zh)
Inventor
林国松
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SHENZHEN TIAN ZHI LIN AGRICULTURAL TECHNOLOGY DEVELOPMENT Co Ltd
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SHENZHEN TIAN ZHI LIN AGRICULTURAL TECHNOLOGY DEVELOPMENT Co Ltd
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Priority to CN201410489085.4A priority Critical patent/CN104262049A/en
Publication of CN104262049A publication Critical patent/CN104262049A/en
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/10—Process efficiency
    • Y02P20/133—Renewable energy sources, e.g. sunlight
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Biochemistry (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a preparation method of a biological organic fertilizer. The preparation method of the biological organic fertilizer comprises two steps: (1) organic raw material fermentation and (2) biological organic fertilizer preparation, wherein a fermenting microbial agent composed of saccharomycetes, actinomycetes and lactobacillus plantarum is adopted in the organic raw material fermentation process; a functional microbial agent composed of bacillus subtillis, azospirillum, rhizobium and streptomyces griseus rust mutant is adopted in the biological organic fertilizer preparation process. The preparation method of the biological organic fertilizer has the advantages of effectiveness in promoting the growth of plants, high cost performance and the like.

Description

A kind of preparation method of Biological organic chemical fertilizer
Technical field
That the present invention relates to is a kind of preparation method of chemical fertilizer, and that be specifically related to is a kind of preparation method of Biological organic chemical fertilizer.
Background technology
Organic-inorganic compound fertilizer refers to the composite fertilizer containing organic substance and inorganic nutrients.Organic substance mostly adopt processed after organic fertilizer (as feces of livestock and poultry, municipal wastes organism, mud, stalk, wood chip, food-processing waste material etc.) and containing organic material (peat composed of rotten mosses, weathered coal, brown coal, humic acid etc.), the material adding microbiobacterial agent and stimulating growth also had, be called Organic active fertilizer or biological slow-release fertilizer, inorganic nutrients part mainly chemical fertilizer.
Microorganism just has activity under certain environment, and this environmental requirement is very high.Chemical fertilizer great majority are salts, and solubleness is very high, have been certain to kill or restraining effect to microbic activity.What thus adopt in the course of processing is dry material, thus makes the organic-inorganic compound fertilizer made lack moisture content, avoids the kill activity that salt plays Institute of Micro-biology.
But the organic-inorganic compound fertilizer of some kind is after being manured into soil, the fertilizer solution of high density can not bring back to life the microorganism added, and is also likely killed by the microorganism added, so the fertilizer efficiency of active mixed organic manure and biological organic composite mixing fertilizer can not be affirmed.
Summary of the invention
The object of the invention is to the unsubstantiated problem of fertilizer efficiency solving existing active mixed organic manure and biological organic composite mixing fertilizer, the preparation method of the Biological organic chemical fertilizer providing a kind of fertilizer efficiency splendid.
Realize technical scheme of the present invention as follows:
A preparation method for Biological organic chemical fertilizer, is made up of following steps:
(1) organic raw material fermentation
Organic raw material, by after heating, oven dry, fragmentation, sterilizing, adds fermenting agent in organic raw material, and add the living bacteria count of >=0.3 hundred million in 1g organic raw material, under 25 DEG C ~ 35 DEG C conditions, fermentation reactor system 7 ~ 10 days, makes fertilizer;
(2) preparation of Biological organic chemical fertilizer
Fertilizer and inorganic nutrients carried out pulverize, stir, granulation, oven dry, cooling, screening process, with function microbial inoculum, coating process is carried out to the particle sieved out, as living bacteria count >=0.2 hundred million/g, namely makes Biological organic chemical fertilizer;
Described fermenting agent is made up of actinomycetes, plant lactobacillus and yeast, and described actinomycetes account for 20 ~ 30% of total viable count, and plant lactobacillus accounts for 10 ~ 20% of total viable count, and yeast accounts for 50 ~ 70% of total viable count.
Described functional microorganism microbial inoculum is made up of Bacillus subtillis, azospirillum, root nodule bacterium, the mutation of streptomyces griseus rust, wherein, Bacillus subtillis accounts for 30 ~ 50% of total viable count, azospirillum accounts for 15 ~ 30% of total viable count, root nodule bacterium account for 15 ~ 30% of total viable count, and the mutation of streptomyces griseus rust accounts for 20 ~ 30% of total viable count.
Further, adopt the dedusting of level Four settling pocket, the dedusting of moisture film settling pocket, sending down abnormally ascending in the process dry in described step (2), cooled, channel-type water film dedusting, sending down abnormally ascending, water film dedusting water returns granulation.
Further, after drying in described step (1), moisture content is 30% ~ 40%.
Preferably, fertilizer is made again through overcuring after fermentation in described step (1).
As another optimal way, the fermentation in described step (1) adopts the fermentation of solar energy greenhouse booth.
As one preferably set-up mode, described inorganic nutrients is by N, P 2o 5, K 2o forms; The mass ratio of described fertilizer and this inorganic nutrients is 17 ~ 18:2 ~ 3.
In order to reach better effect, in described step (2), oven dry, cooled moisture content are 20% ~ 30%.
In order to effectively ensure the effect of Biological organic chemical fertilizer of the present invention, the pH value of described Biological organic chemical fertilizer is 5.5 ~ 8.5.
Further, excrement colibacillus group number≤100/g in described Biological organic chemical fertilizer, returns early worm's ovum mortality ratio >=95%.
The present invention has the following advantages and beneficial effect:
1, the present invention by the extra-granular function microbial inoculum that is mixed at fertilizer and inorganic nutrients to carrying out coating process, effective practical function microbial inoculum acts preferentially on plant, avoid bacterial classification in fertilizer to impact bacterial classification in function microbial inoculum, function microbial inoculum promotes that the effect of plant strain growth is more remarkable.
2, the present invention utilizes microorganism chemical fertilizer to have the ability of fast decoupled organic matter, the nutritive element that the organic matter decomposition such as chicken manure, cow dung, pig manure become plant directly to utilize, make it more easily to be absorbed by plants, while reducing production cost, solve biological waste and pile up the ecological damage caused.
3, the biological chemical fertilizer that the present invention produces can not only make soil supplement new organic matter, and draw in plant rhizosphere microecosystem and grow a large amount of beneficial microorganism, plant growth is impelled to be in benign recurrent state, make many microorganisms be in secondary status, be difficult to the advantage group kind forming crop growth.Suppress harmful microbe breeding, prevention, minimizing disease and pest occur; Reduce and even do not use fertilizer and pesticide, produce real green organic farm products.
4, the present invention adopts organic and inorganic, biological three combination configurations, and mutually supplement, mutual synergy, significantly improves chemical fertilizer utilization ratio, reduces chemical fertilizer to the pollution of environment, increases crop yield further, improve crop quality, realize agricultural production and income.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but embodiments of the present invention are not limited thereto.
Embodiment 1
A preparation method for Biological organic chemical fertilizer, is made up of following steps:
(1) organic raw material fermentation
Organic raw material is by after heating, oven dry, fragmentation, sterilizing, fermenting agent is added in organic raw material, add the living bacteria count of >=0.3 hundred million in 1g organic raw material, under 25 DEG C ~ 35 DEG C conditions, fermentation reactor system 7 ~ 10 days, makes fertilizer again through overcuring process after fermentation.
In step (1), fermentation adopts fermentation reactor system; The fermentation of solar energy greenhouse booth is adopted during fermentation.And the rear moisture content of organic raw material oven dry is 30% ~ 40% in this step.
Described in this step (1), fermenting agent is made up of actinomycetes, plant lactobacillus and yeast, and described actinomycetes account for 20 ~ 30% of total viable count, and plant lactobacillus accounts for 10 ~ 20% of total viable count, and yeast accounts for 50 ~ 70% of total viable count.
(2) preparation of Biological organic chemical fertilizer
Fertilizer and inorganic nutrients carried out pulverize, stir, granulation, oven dry, cooling, screening process, with function microbial inoculum, coating process is carried out to the particle sieved out, as living bacteria count >=0.2 hundred million/g, namely makes Biological organic chemical fertilizer.
Additionally use the dedusting of level Four settling pocket, the dedusting of moisture film settling pocket, sending down abnormally ascending in the process dry in described step (2), cooled, channel-type water film dedusting, sending down abnormally ascending, water film dedusting water returns the step of granulation.In channel-type water film dedusting, sending down abnormally ascending process, also have gas to discharge.
Described in step of the present invention (2), the mass ratio of fertilizer and this inorganic nutrients is 17 ~ 18:2 ~ 3, and described inorganic nutrients is by N, P 2o 5, K 2o forms.And oven dry, cooled moisture content are 20% ~ 30% in step (2).
The present invention is 5.5 ~ 8.5 by the pH value of the Biological organic chemical fertilizer that step (2) is prepared; Be excrement colibacillus group number≤100/g in this Biological organic chemical fertilizer, return early worm's ovum mortality ratio >=95%.
In described step (2), functional microorganism microbial inoculum is made up of Bacillus subtillis, azospirillum, root nodule bacterium, the mutation of streptomyces griseus rust, wherein, Bacillus subtillis accounts for 30 ~ 50% of total viable count, azospirillum accounts for 15 ~ 30% of total viable count, root nodule bacterium account for 15 ~ 30% of total viable count, and the mutation of streptomyces griseus rust accounts for 20 ~ 30% of total viable count.
The bacterial classification adopted in the present invention is all existing commercially available bacterial classifications.Described Bacillus subtillis is being buied on the market, the BS5000 that this Bacillus subtillis adopts Bai Fude bio tech ltd, Wuxi to produce; Azospirillum adopts the Brasil diazotrophic spirillum SW51 (NCAIM/P/B 001293) cultivating and be deposited in state-run agricultural and industrial microorganism and cyropreservation center; What root nodule bacterium adopted is Qiyang root nodule bacterium, and it is CGMCC 5171 at the preserving number at China Committee for Culture Collection of Microorganisms's common micro-organisms center; The mutation of streptomyces griseus rust adopts the streptomyces griseus rust mutation being preserved in Institute of Biology, Gansu Academy of Sciences's Organism Depositary, and its preserving number is GSICC 41920.
Actinomycetes, plant lactobacillus, yeast are all from China Committee for Culture Collection of Microorganisms's common micro-organisms center, the actinoplanes (Actinoplanes sp.) of these actinomycetes to be deposit number be CGMCC 4.1150, this plant lactobacillus (Lactobacillus plantarum subsp. plantarum) deposit number is CGMCC 1.510, and this yeast deposit number is the yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) of CGMCC 2.366.
In the present invention, the concrete preparation method of functional microorganism microbial inoculum is as follows:
First, cultivated in slant medium respectively by the bacterial classification buied and bring back to life for 3 ~ 5 days, this slant medium adopts solid-state beef-protein medium.
Secondly, the Bacillus subtillis after bringing back to life, azospirillum, root nodule bacterium and streptomyces griseus rust mutation bacterium are taken out respectively in slant medium, and once cultivate respectively under earthquake condition, second incubation, cultivate for three times, fermentation culture.
Described once cultivation, second incubation, to cultivate for three times and the culture temperature of fermentation culture is 28 DEG C ~ 32 DEG C, the frequency of concussion is 250rpm, and concrete culturing step of the present invention is as follows:
Bacterial classification 2 ~ 3 ring after resurrection in slant medium is inoculated in the nutrient solution once cultivating 400ml and shakes cultivation two days, ratio according to 10% shakes cultivation one day by strain inoculation in a subculture to second incubation liquid, then by bacterial classification in second incubation liquid according to 10% ratio be inoculated in three nutrient solutions and shake cultivation one day.
By the bacterial classification in three nutrient solutions according to 10% ratio add in fermentor tank and carry out fermentation culture, this fermentation culture is until the bacterial classification quantity in nutrient solution is greater than 2,000,000,000/ml, and in the present embodiment, the number of days of fermentation culture is 3 ~ 10 days.
Then, the nutrient solution of four kinds of bacterial classifications is mixed according to proportioning.
Wherein, the substratum that once cultivation, second incubation, three cultivations adopt is identical, is all adopt liquid beef-protein medium.The substratum that described fermentation culture adopts is nutrient broth medium.Above-mentioned solid-state beef-protein medium, liquid beef-protein medium, nutrient broth medium are all existing formulas, and thus the concrete proportioning of concrete above-mentioned substratum does not repeat them here.
In the functional microorganism microbial inoculum of the present embodiment, Bacillus subtillis account for total viable count 45%, azospirillum account for total viable count 20%, root nodule bacterium account for total viable count 15%, the mutation of streptomyces griseus rust accounts for 25% of total viable count.
In the fermenting agent of the present embodiment, actinomycetes account for 20% of total viable count, and plant lactobacillus accounts for 20% of total viable count, and yeast accounts for 60% of total viable count.
Using method of the present invention is:
At seeding time, Biological organic chemical fertilizer of the present invention and seed are broadcast in soil according to the weight ratio that mass ratio is 1:10.Or apply fertilizer at fruit tree root according to the amount of 50g/ strain, or apply fertilizer in soil according to the amount of 6kg/ mu.And once topdress every month, identical when consumption and sowing.
Embodiment 2
The difference of the present embodiment and embodiment 1 is:
(1) mass ratio of fertilizer described in the present embodiment and this inorganic nutrients is 18:2.
(2) described inorganic nutrients is by N, P 2o 5, K 2o forms; This N, P 2o 5, K 2weight ratio between O is 3:10:7.
(3) fermenting agent used in described fertilizer, by the actinomycetes accounting for total viable count 30%, accounts for the plant lactobacillus of total viable count 20%, accounts for the yeast composition of total viable count 50%.
(4) described functional microorganism microbial inoculum by account for total viable count 34% Bacillus subtillis, account for the azospirillum of 22% of total viable count, account for the root nodule bacterium of 17% of total viable count, and account for total viable count 27% the mutation of streptomyces griseus rust composition.
Embodiment 3
The difference of the present embodiment and embodiment 1 is:
(1) mass ratio of fertilizer described in the present embodiment and this inorganic nutrients is 17:2.
(2) described inorganic nutrients is by N, P 2o 5, K 2o forms; This N, P 2o 5, K 2weight ratio between O is 13:6:8.
(3) fermenting agent used in described fertilizer, by the actinomycetes accounting for total viable count 20%, accounts for the plant lactobacillus of total viable count 10%, accounts for the yeast composition of total viable count 70%
(4) described functional microorganism microbial inoculum by account for total viable count 50% Bacillus subtillis, account for the azospirillum of 15% of total viable count, account for the root nodule bacterium of 15% of total viable count, and account for total viable count 20% the mutation of streptomyces griseus rust composition.
Contrast experiment
(1) directly by planting seed in soil.
(2) the commercially available microbiobacterial agent of Promoting plant growth according to its using method, fertilising can being carried out to plant and use is adopted.This commercially available microbiobacterial agent adopts the EM stoste developed cooperatively by Japanese EM mechanism and Chinese Kang Yuan oasis biotechnology.
The present invention adopts above-described embodiment and contrast experiment to test different types of plant respectively, and table 1 represents the experimental result of field crop corn, and table 2 represents the experimental result of spinach, and table 3 represents the experimental result of apple plants.
Biological organic chemical fertilizer of the present invention adopts organic and inorganic, biological three combination configurations, and mutually supplement, mutual synergy, effectively can increase agriculture value, field crop can increase production more than 20%, and vegetables can increase production more than 30%, and fruit sugar degree improves 5%, and effect is extremely remarkable.
According to above embodiment, just the present invention can well be realized.

Claims (9)

1. a preparation method for Biological organic chemical fertilizer, is characterized in that, is made up of following steps:
(1) organic raw material fermentation
Organic raw material, by after heating, oven dry, fragmentation, sterilizing, adds fermenting agent in organic raw material, and add the living bacteria count of >=0.3 hundred million in 1g organic raw material, under 25 DEG C ~ 35 DEG C conditions, fermentation reactor system 7 ~ 10 days, makes fertilizer;
(2) preparation of Biological organic chemical fertilizer
Fertilizer and inorganic nutrients carried out pulverize, stir, granulation, oven dry, cooling, screening process, with function microbial inoculum, coating process is carried out to the particle sieved out, as living bacteria count >=0.2 hundred million/g, namely makes Biological organic chemical fertilizer;
Described fermenting agent is made up of actinomycetes, plant lactobacillus and yeast, and described actinomycetes account for 20 ~ 30% of total viable count, and plant lactobacillus accounts for 10 ~ 20% of total viable count, and yeast accounts for 50 ~ 70% of total viable count; Described functional microorganism microbial inoculum is made up of Bacillus subtillis, azospirillum, root nodule bacterium, the mutation of streptomyces griseus rust, wherein, Bacillus subtillis accounts for 30 ~ 50% of total viable count, azospirillum accounts for 15 ~ 30% of total viable count, root nodule bacterium account for 15 ~ 30% of total viable count, and the mutation of streptomyces griseus rust accounts for 20 ~ 30% of total viable count.
2. the preparation method of a kind of Biological organic chemical fertilizer according to claim 1, settling pocket dedusting is adopted, the dedusting of moisture film settling pocket, sending down abnormally ascending in the process it is characterized in that, dry in described step (2), cooling, channel-type water film dedusting, sending down abnormally ascending, water film dedusting water returns granulation.
3. the preparation method of a kind of Biological organic chemical fertilizer according to claim 2, is characterized in that, after drying in described step (1), moisture content is 30% ~ 40%.
4. the preparation method of a kind of Biological organic chemical fertilizer according to claim 3, is characterized in that, makes fertilizer again in described step (1) after fermentation through overcuring.
5. the preparation method of a kind of Biological organic chemical fertilizer according to claim 4, is characterized in that, the fermentation in described step (1) adopts the fermentation of solar energy greenhouse booth.
6. the preparation method of a kind of Biological organic chemical fertilizer according to any one of Claims 1 to 5, is characterized in that, described inorganic nutrients is by N, P 2o 5, K 2o forms; The mass ratio of described fertilizer and this inorganic nutrients is 17 ~ 18:2 ~ 3.
7. the preparation method of a kind of Biological organic chemical fertilizer according to claim 6, is characterized in that, in described step (2), oven dry, cooled moisture content are 20% ~ 30%.
8. the preparation method of a kind of Biological organic chemical fertilizer according to claim 7, is characterized in that, the pH value of described Biological organic chemical fertilizer is 5.5 ~ 8.5.
9. the preparation method of a kind of Biological organic chemical fertilizer according to claim 8, is characterized in that, excrement colibacillus group number≤100/g in described Biological organic chemical fertilizer, returns early worm's ovum mortality ratio >=95%.
CN201410489085.4A 2014-09-23 2014-09-23 Preparation method of biological organic fertilizer Pending CN104262049A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113735649A (en) * 2020-11-30 2021-12-03 云南省微生物发酵工程研究中心有限公司 Preparation method of bio-organic fertilizer
CN114097519A (en) * 2021-12-06 2022-03-01 丽水市森林资源保护管理总站 Chinese torreya planting method
WO2022259143A1 (en) * 2021-06-09 2022-12-15 Srinivas Boosa Integrated plant growth management composition and method of preparation thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1346819A (en) * 2001-11-09 2002-05-01 现代农装科技股份有限公司 Composite fertilizer and its preparing process and equipment
CN1631854A (en) * 2004-11-26 2005-06-29 栖霞市宏翔微生物肥料有限公司 Multifunctional biological organic and inorganic blended fertilizer and its production process
CN1840512A (en) * 2005-03-28 2006-10-04 重庆景初微生物有机肥料有限责任公司 Process for preparing microbial manure
CN101870608A (en) * 2010-06-21 2010-10-27 山东宝源生物有限公司 Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1346819A (en) * 2001-11-09 2002-05-01 现代农装科技股份有限公司 Composite fertilizer and its preparing process and equipment
CN1631854A (en) * 2004-11-26 2005-06-29 栖霞市宏翔微生物肥料有限公司 Multifunctional biological organic and inorganic blended fertilizer and its production process
CN1840512A (en) * 2005-03-28 2006-10-04 重庆景初微生物有机肥料有限责任公司 Process for preparing microbial manure
CN101870608A (en) * 2010-06-21 2010-10-27 山东宝源生物有限公司 Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113735649A (en) * 2020-11-30 2021-12-03 云南省微生物发酵工程研究中心有限公司 Preparation method of bio-organic fertilizer
WO2022259143A1 (en) * 2021-06-09 2022-12-15 Srinivas Boosa Integrated plant growth management composition and method of preparation thereof
CN114097519A (en) * 2021-12-06 2022-03-01 丽水市森林资源保护管理总站 Chinese torreya planting method

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